Negative pressure-based adsorption system and method, and biochemical substance analysis method and device

Abstract

A negative pressure-based adsorption system and method, and a biochemical substance analysis method and device. The adsorption system comprises a gas storage module, a first power module, and a first adsorption module. The first power module is communicated with the gas storage module and is configured to generate negative pressure in the gas storage module. The first adsorption module is configured to be communicated with the gas storage module. The gas storage module is configured to convey negative pressure to the first adsorption module, so that the first adsorption module uses the negative pressure to adsorb a target object.

Description

Negative pressure based adsorption system and method, biochemical substance analysis method and device TECHNICAL FIELD

[0001] The present application relates to negative pressure control, in particular to a negative pressure based adsorption system, a negative pressure based adsorption method, a biochemical substance analysis system and a biochemical substance analysis device. BACKGROUND

[0002] In the related art, a target object can be first placed on a support platform, and then adsorbed on the support platform under a negative pressure state, and then a required operation is performed on the target object. For example, in the field of biochemistry or medicine, a microfluidic chip can be first placed on a chip platform, and then adsorbed on the chip platform under a negative pressure state, and then a fluid is introduced into the microfluidic chip for biochemical reaction, and then the reacted fluid is detected.

[0003] However, in order to keep the chip platform in an adsorbed state, the vacuum pump needs to be started all the time, and long-term continuous operation will reduce the service life of the vacuum pump.

[0004] SUMMARY

[0005] Therefore, it is necessary to provide a negative pressure based adsorption system, a negative pressure based adsorption method, a biochemical substance analysis system and a biochemical substance analysis device.

[0006] The first aspect of the present application provides a negative pressure based adsorption system, comprising a gas storage module, a first power module and a first adsorption module. The first power module is in communication with the gas storage module and is configured to generate a negative pressure in the gas storage module. The first adsorption module is configured to be in communication with the gas storage module, and the gas storage module is configured to deliver a negative pressure to the first adsorption module, so that the first adsorption module adsorbs a target object by using the negative pressure.

[0007] The second aspect of the present application provides a negative pressure based adsorption method, comprising: establishing a connection between a first power module and a gas storage module; the first power module is started and generates a negative pressure in the gas storage module; the first power module is closed and a connection between the gas storage module and a first adsorption module is established; the gas storage module delivers a negative pressure to the first adsorption module, so that the first adsorption module adsorbs a target object by using the negative pressure.

[0008] The third aspect of the present application provides a biochemical substance analysis method, comprising: establishing a connection between a first power module and a gas storage module; starting the first power module and generating a negative pressure in the gas storage module; closing the first power module and establishing a connection between the gas storage module and a first adsorption module; the gas storage module transports the negative pressure to the first adsorption module, so that the first adsorption module adsorbs a carrier by using the negative pressure; performing a first operation or a second operation on the carrier, the first operation is to make a biochemical reaction of a fluid in the carrier, and the second operation is to detect the fluid in the carrier.

[0009] The fourth aspect of the present application provides a biochemical substance analysis device, comprising a negative pressure-based adsorption system as described above. The adsorption system is configured to adsorb the target object, which is a carrier.

[0010] The adsorption system of the present application introduces a gas storage module, so that even if the first power module is closed, the negative pressure of the adsorption system changes slowly, so that the first adsorption module can continuously and stably adsorb the target object to facilitate subsequent operations on the target object, and also helps to improve the service life of the first power module. BRIEF DESCRIPTION OF DRAWINGS

[0011] FIG. 1 is a module architecture diagram of the adsorption system provided by an embodiment of the present application.

[0012] FIG. 2 is a module architecture diagram of the adsorption system shown in FIG. 1 when performing a first adsorption method.

[0013] FIG. 3 is a module architecture diagram of the adsorption system shown in FIG. 1 when performing a second adsorption method.

[0014] FIG. 4 is a module architecture diagram of the adsorption system shown in FIG. 1 when performing a third adsorption method.

[0015] FIG. 5 is a structural schematic diagram of the adsorption system shown in FIG. 1 in some embodiments.

[0016] FIG. 6 is a diagram of the relationship between negative pressure and time when the adsorption system shown in FIG. 5 performs different pressure building methods.

[0017] FIG. 7 is a structural schematic diagram of the adsorption system shown in FIG. 5 after a shielding component is provided on the carrier platform.

[0018] FIG. 8 is a structural schematic diagram of the adsorption system shown in FIG. 1 in another embodiment.

[0019] FIG. 9 is a module architecture diagram of a biochemical substance analysis device provided by an embodiment of the present application.

[0020] Main component symbol explanation Biochemical substance analysis device 1 Gas storage module 10 Gas tank 10a Gas pressure sensing device 11 Gas pressure sensor 11a Power module 20 Piston pump 20a, 20b First power module 21 Second power module 22 Mth power module 2M Adsorption module 30 Carrying platform 30a, 30b, 30c, 30d First adsorption module 31 Second adsorption module 32 Nth adsorption module 3N Selection module 40 Three-way electromagnetic valve 40a, 40b, 40c, 40d First selection module 41 Second selection module 42 Nth selection module 4N Gas pressure sensor 50a, 50b, 50c, 50d First sensing device 51 Second sensing device 52 Nth sensing device 5N Gas-liquid separator 60a, 60b, 60c, 60d First gas-liquid separation device 61 Second gas-liquid separation device 62 Nth gas-liquid separation device 6N Filter 70a, 70b, 70c, 70d First filtering device 71 Second filtering device 72 Nth filtering device 7N Shielding component 80 Gas channel 81 Five-way joint 90 Adsorption system 100 First pipeline L1 Second pipeline L2 Third pipeline L3 Fourth pipeline L4 Fifth pipeline L5 Sixth pipeline L6 Opening H

[0021] The following detailed description will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be described clearly and completely in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments.

[0023] It should be understood that when an element or layer is referred to as being "on" or "connected to" another element or layer, it can be directly on or connected to the other element or layer or intervening elements or layers can be present. In contrast, when an element is referred to as being "directly on" or "directly connected to" another element, there are no intervening elements or layers present. Like numbers refer to like elements throughout. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0024] It should be understood that, although the process shown in the flow chart includes a logical sequence of steps, in some cases, the order of the steps can be changed, or some steps can be performed in parallel, without departing from the scope of the present application. The method disclosed in the embodiments of the present application includes one or more steps or actions for implementing the method. The method steps and / or actions can be interchanged with each other without departing from the scope of the claims. Unless the specific order of steps or actions is specified, the order and / or use of specific steps and / or actions can be modified without departing from the scope of the claims.

[0025] Referring to FIG. 1, an embodiment of the present application provides a negative pressure-based suction system 100 for suctioning a target object (not shown) to facilitate subsequent operations on the target object. The suction system 100 includes a gas storage module 10, a power module 20, and a suction module 30.

[0026] The power module 20 is a functional module in the suction system 100 for generating negative pressure. The power module 20 can include M power modules, M≥1, i.e., the power module 20 includes a first power module 21 to an Mth power module 2M. When the power module 20 includes multiple power modules, each power module can work independently. The structure of each power module can be the same or different. In some embodiments, the power module 20 includes a first power module 21 and a second power module 22. When one of the first power module 21 and the second power module 22 is working, the other one can not work. The first power module 21 and the second power module 22 can work in sequence or in parallel. "Parallel work" means that the working time of the first power module 21 and the second power module 22 completely overlaps or at least partially overlaps on the time axis, thereby optimizing the total time required to generate the target negative pressure.

[0027] The gas storage module 10 is a functional module in the suction system 100 for storing negative pressure. The first power module 21 and the second power module 22 can both be in communication with the gas storage module 10. Wherein, the "connection" or "communication" referred to in the present application means that the modules are connected or communicated with each other through pipes or other suitable components. The gas storage module 10 can be a container suitable for storing gas, such as a gas tank.

[0028] The adsorption module 30 is a functional module in the adsorption system 100 for adsorbing and fixing the target object. The adsorption module 30 can include N adsorption modules, N≥M, i.e., the adsorption module 30 includes the first adsorption module 31 to the Nth adsorption module 3N. Each adsorption module can be independent of each other and adsorb different target objects. The structure of each adsorption module can be the same or different. In some embodiments, the adsorption module 30 includes the first adsorption module 31 and the second adsorption module 32. At least one of the first adsorption module 31 and the second adsorption module 32 is selectively communicated to the gas storage module 10. For example, the first adsorption module 31 and the second adsorption module 32 can adsorb the target object in sequence, or can adsorb the target object in parallel. The first adsorption module 31 and the second adsorption module 32 can be components having an opening H (shown in FIG. 7) and being adapted to provide support for the target object, and when the external environment supplies negative pressure to the first adsorption module 31 or the second adsorption module 32, the negative pressure acts on the target object through the opening H, so that the first adsorption module 31 or the second adsorption module 32 can adsorb the target object by using the negative pressure.

[0029] The first power module 21 can be communicated with the gas storage module 10 and generate negative pressure in the gas storage module 10. When the first adsorption module 31 is communicated with the gas storage module 10, the gas storage module 10 can supply negative pressure to the first adsorption module 31, so that the first adsorption module 31 can adsorb the target object by using the negative pressure. For example, the first power module 21 can drive the gas to move in the opposite direction, which means that the gas is extracted to flow from the gas storage module 10 to the first power module 21. The first power module 21 can be various types of pumps that can drive the gas to move, such as a syringe pump, a plunger pump, a diaphragm pump, a gear pump, a peristaltic pump, or a vacuum pump, etc.

[0030] The second power module 22 can be in communication with the gas storage module 10 and generate negative pressure within the gas storage module 10. When the second adsorption module 32 is in communication with the gas storage module 10, the gas storage module 10 can deliver negative pressure to the second adsorption module 32, so that the second adsorption module 32 adsorbs another target object by using the negative pressure. For example, the second power module 22 can drive the gas to move in the reverse direction, which means the direction of extracting the gas to flow from the gas storage module 10 to the second power module 22. At least one of the first power module 21 and the second power module 22 is selectively activated. For example, the first power module 21 and the second power module 22 can work in parallel to generate negative pressure within the gas storage module 10, and the second power module 22 can also be a backup power module, which is communicated to the gas storage module 10 only when needed (for example, when the pressure building speed of the first power module 21 is insufficient, the second power module 22 is communicated to the gas storage module 10), and both of them build pressure at the same time, thereby improving the reliability of the adsorption system 100. The second power module 22 can be various types of pumps that can drive the gas to move, such as a syringe pump, a plunger pump, a diaphragm pump, a gear pump, a peristaltic pump, or a vacuum pump, etc.

[0031] In some embodiments, the adsorption system 100 can further include a selection module 40. The selection module 40 can include N selection modules, i.e., the selection module 40 includes a first selection module 41 to an Nth selection module 4N. Each selection module can work independently. The structure of each selection module can be the same or different. Each selection module can communicate the gas storage module 10 with different adsorption modules, so as to realize the selection of the flow direction of the negative pressure within the gas storage module 10. In some embodiments, the selection module 40 includes a first selection module 41 and a second selection module 42. The first selection module 41 can communicate or disconnect the gas storage module 10 with the first adsorption module 31, and the second selection module 42 can communicate or disconnect the gas storage module 10 with the second adsorption module 32. Among them, the first selection module 41 or the second selection module 42 can be various types of electromagnetic valves, selection valves (such as rotary valves), etc. valves or combinations thereof.

[0032] The first power module 21 and the gas storage module 10 can be connected through the first pipeline L1, the gas storage module 10 and the first selection module 41 can be connected through the second pipeline L2, and the first selection module 41 and the first adsorption module 31 can be connected through the third pipeline L3. Similarly, the second power module 22 and the gas storage module 10 can be connected through the fourth pipeline L4, the gas storage module 10 and the second selection module 42 can be connected through the fifth pipeline L5, and the second selection module 42 and the second adsorption module 32 can be connected through the sixth pipeline L6. In the adsorption system 100, the internal volume of the gas storage module 10, the first pipeline L1, the second pipeline L2 and the third pipeline L3 connected to all components of the first power module 21 are related to the volume of the four. The internal volume of the adsorption system 100 is defined as V, the leak rate between the adsorption system 100 and the atmosphere is leak rate (sccm), the atmospheric pressure is P atm , the negative pressure change of the adsorption system 100 in the time Δt is ΔP, and the calculation formula of ΔP is as follows:

[0033] From the above calculation formula, under the condition that the leak rate of the adsorption system 100 is constant, in the same time Δt, if the internal volume V of the adsorption system 100 is larger, the negative pressure change ΔP is slower, that is, the speed of the negative pressure drop in the adsorption system 100 is slower.

[0034] Compared with the scheme of directly connecting the power module, the selection module and the adsorption module through the pipeline (the internal volume of the pipeline is generally only in the milliliter level, and the negative pressure change of the adsorption system is fast after the power module is closed, so that the power module needs to work all the time to continuously adsorb the target object), the application introduces the gas storage module 10 with a larger volume, so that the negative pressure change of the adsorption system 100 is slow even after the first power module 21 is closed, so that the first adsorption module 31 can continuously and stably adsorb the target object to facilitate subsequent operation on the target object. Moreover, the first power module 21 does not need to work all the time, which is also conducive to improving the service life of the first power module 21. The volume of the gas storage module 10 is defined as V1, the sum of the volumes of the first pipeline L1, the second pipeline L2 and the third pipeline L3 is V2, and the volume of the gas storage module 10 can be set to be in the level of liters, while the volume of the pipeline is generally in the level of milliliters, that is, V1 is much larger than V2, so that the negative pressure change of the adsorption system 100 is slow even after the first power module 21 is closed. Similarly, the sum of the volumes of the fourth pipeline L4, the fifth pipeline L5 and the sixth pipeline L6 is V3, and V1 is much larger than V3, so that the negative pressure change of the adsorption system 100 is slow even after the second power module 22 is closed.

[0035] In some embodiments, N can be greater than M, i.e. the number of power modules is less than the number of adsorption modules. Compared with the scheme of directly connecting the power module, the selection module and the adsorption module through the pipeline (the power module, the selection module and the adsorption module need to be connected one by one, i.e. each adsorption module needs to be matched with a power module), the application can appropriately reduce the number of power modules by introducing the gas storage module 10, thereby reducing the cost.

[0036] Further, the adsorption system 100 can further include a gas pressure sensing device 11 in communication with the gas storage module 10. The gas pressure sensing device 11 can be used to sense the negative pressure value in the gas storage module 10. The first power module 21 can also generate negative pressure in the gas storage module 10 again when the negative pressure value sensed by the gas pressure sensing device 11 is higher than the preset value, thereby reducing the negative pressure value in the gas storage module 10 so that the first adsorption module 31 can continuously and stably adsorb the target object.

[0037] In some embodiments, the adsorption system 100 can further include N sensing devices and alarm devices (not shown in the figure), i.e. the number of sensing devices corresponds to the number of adsorption modules. For example, the adsorption system 100 can include a first sensing device 51 connected between the first adsorption module 31 and the first selection module 41, a second sensing device 52 connected between the second adsorption module 32 and the second selection module 42, and an Nth sensing device 5N connected between the Nth adsorption module 3N and the Nth selection module 4N. The first sensing device 51 is a gas pressure sensor connected to the third pipeline L3, which can sense the negative pressure value in the third pipeline L3. The position and function of the second sensing device 52 are the same. If the adsorption system 100 does not leak, the sum of the negative pressure values measured by the N sensing devices is the negative pressure change ΔP. On the contrary, if the adsorption system 100 leaks, the sum of the negative pressure values measured by the N sensing devices will exceed the negative pressure change ΔP. The alarm device is electrically connected with the gas pressure sensing device 11 and each sensing device. The alarm device can calculate the negative pressure change through the negative pressure value sensed by the gas pressure sensing device 11, and then compare the negative pressure change with the sum of the negative pressure values measured by each sensing device, and generate an alarm signal (such as flashing of the alarm indicator or sounding of the alarm) when it is judged that the adsorption system 100 leaks. The alarm device can also compare the negative pressure values measured by each sensing device, and when the negative pressure value measured by one of the sensing devices is significantly lower than the negative pressure values measured by the other sensing devices, it indicates that the negative pressure value of the corresponding pipeline does not reach the predetermined value within the specified time, thereby generating an alarm signal.

[0038] In another embodiment, the first sensing device 51 may also be a flow sensor connected to the third pipe L3, which can sense the gas flow rate within the third pipe L3. The location and function of the second sensing device 52 are similar. The alarm device can also determine when the gas flow rate exceeds a threshold value. If this threshold value is exceeded, it indicates that the corresponding adsorption module has failed to adsorb the target object, thereby generating an alarm signal.

[0039] The adsorption system 100 may also include N gas-liquid separation devices, i.e., the number of gas-liquid separation devices corresponds to the number of adsorption modules. For example, the adsorption system 100 may include a first gas-liquid separation device 61 connected between the first adsorption module 31 and the first selection module 41, a second gas-liquid separation device 62 connected between the second adsorption module 32 and the second selection module 42, and an Nth gas-liquid separation device 6N connected between the Nth adsorption module 3N and the Nth selection module 4N. For example, the first gas-liquid separation device 61 is connected to the third pipeline L3 and can prevent liquid from flowing from the first adsorption module 31 to the first selection module 41 and the gas storage module 10. The position and function of the second gas-liquid separation device 62 are similar. The first gas-liquid separation device 61 and the second gas-liquid separation device 62 can be gas-liquid separators that block the passage of liquid.

[0040] The adsorption system 100 may also include N filter devices, i.e., the number of filter devices corresponds to the number of adsorption modules. For example, the adsorption system 100 may include a first filter device 71 connected to the first selection module 41, a second filter device 72 connected to the second selection module 42, and an Nth filter device 7N connected to the Nth selection module 4N. The first filter device 71 prevents impurities in the air from entering the third pipeline L3. The position and function of the second filter device 72 are similar. The first filter device 71 and the second filter device 72 may be filters, such as filter screens, that block the entry of impurities.

[0041] In some embodiments, the first power module 21 can also generate positive pressure within the gas storage module 10, thereby allowing the gas storage module 10 to deliver positive pressure to the first adsorption module 31. For example, the first power module 21 is reversible and can therefore drive gas movement in a positive direction, which refers to the direction in which gas is drawn from the first power module 21 to the gas storage module 10. Under the action of positive pressure, residue (such as impurities and dust) in the first adsorption module 31 can be removed under positive pressure. In other embodiments, the adsorption system 100 may further include a positive pump to generate positive pressure within the gas storage module 10.

[0042] The following will describe the three adsorption methods of the adsorption system 100 in combination with the various functional modules of the adsorption system 100 .

[0043] The first adsorption method

[0044] As shown in Figure 2, under the first adsorption method, only the first power module 21 is in operation, and other power modules, such as the second power module 22, are inoperative or can even be omitted from the adsorption system 100. This adsorption method is used to adsorb a target object through the first adsorption module 31. Assume that in the initial state, the target object is not placed on the first adsorption module 31 and the first adsorption module 31 is connected to the atmosphere. The first adsorption method can be specifically decomposed into four steps, namely steps 1 to 4. According to different needs, the order of the steps of the above method can be changed, and some steps can be omitted or combined.

[0045] Step 1: Establish a connection between the first power module 21 and the gas storage module 10 . The first power module 21 starts up and generates negative pressure in the gas storage module 10 .

[0046] In some embodiments, when the first power module 21 and the gas storage module 10 are connected through the first pipeline L1 and the gas storage module 10 and the first selection module 41 are connected through the second pipeline L2, this step may also generate negative pressure in the first pipeline L1 and the second pipeline L2.

[0047] In step 2, the first power module 21 is turned off, and the target object is placed on the first adsorption module 31 .

[0048] Step three: establish a connection between the gas storage module 10 and the first adsorption module 31 so that the gas storage module 10 delivers negative pressure to the first adsorption module 31 , thereby enabling the first adsorption module 31 to adsorb the target object using the negative pressure.

[0049] In some embodiments, when the adsorption system 100 includes the first selection module 41, the gas storage module 10 can be connected to the first adsorption module 31 through the first selection module 41. After the first adsorption module 31 uses negative pressure to adsorb the target object, the target object is fixed to the first adsorption module 31, and corresponding operations can be performed on the target object.

[0050] It is understood that after the gas storage module 10 delivers negative pressure to the first adsorption module 31, the negative pressure within the gas storage module 10 itself will decrease. Therefore, a pressure sensing device 11 can be installed within the gas storage module 10. The pressure sensing device 11 can be fully activated or activated in step 3 to sense the negative pressure within the gas storage module 10. When the negative pressure exceeds a preset value, the first power module 21 generates negative pressure within the gas storage module 10 again.

[0051] Step 4: disconnect the gas storage module 10 from the first adsorption module 31 , so that the negative pressure at the first adsorption module 31 is released.

[0052] In some embodiments, when the corresponding operation on the target object is completed, the gas storage module 10 can be disconnected from the first adsorption module 31 by the first selection module 41.

[0053] Second adsorption method

[0054] As shown in FIG. 3, only the first power module 21 works in the second adsorption method, and other power modules such as the second power module 22 do not work or can even be omitted from the adsorption system 100. This adsorption method is used to adsorb different target objects by the first adsorption module 31 and the second adsorption module 32 respectively. Assuming that the target objects are not placed on the first adsorption module 31 and the second adsorption module 32 in the initial state, the first adsorption module 31 and the second adsorption module 32 are both connected to the atmosphere. The second adsorption method can be divided into six steps, i.e. steps one to six. According to different needs, the order of the steps of the above method can be changed, and some steps can be omitted or combined.

[0055] Step one, establish the connection between the first power module 21 and the gas storage module 10, and the first power module 21 starts and generates negative pressure in the gas storage module 10.

[0056] In some embodiments, when the first power module 21 and the gas storage module 10 are connected by the first pipeline L1 and the gas storage module 10 and the first selection module 41 are connected by the second pipeline L2, this step can also generate negative pressure in the first pipeline L1 and the second pipeline L2.

[0057] Step two, the first power module 21 is closed, and the target object is placed on the first adsorption module 31.

[0058] Step three, establish the connection between the gas storage module 10 and the first adsorption module 31, so that the gas storage module 10 transports negative pressure to the first adsorption module 31, so that the first adsorption module 31 adsorbs the target object by using negative pressure.

[0059] In some embodiments, when the adsorption system 100 includes the first selection module 41, the gas storage module 10 can be connected to the first adsorption module 31 by the first selection module 41. When the first adsorption module 31 adsorbs the target object by using negative pressure, the target object is fixed on the first adsorption module 31, and at this time, the corresponding operation can be performed on the target object.

[0060] Step four, place another target object on the second adsorption module 32.

[0061] Step five, establish the connection between the gas storage module 10 and the second adsorption module 32, so that the gas storage module 10 transports negative pressure to the second adsorption module 32, so that the second adsorption module 32 adsorbs the target object by using negative pressure.

[0062] In some embodiments, when the adsorption system 100 further comprises a second selection module 42, the gas storage module 10 can be communicated with the second adsorption module 32 through the second selection module 42. When the second adsorption module 32 adsorbs the target object by using the negative pressure, the target object is fixed on the second adsorption module 32, and at this time, the corresponding operation can be performed on the target object.

[0063] Step six, disconnect the gas storage module 10 from the first adsorption module 31 and the second adsorption module 32, so that the negative pressure at the first adsorption module 31 and the second adsorption module 32 is released respectively.

[0064] In some embodiments, when the corresponding operation on the target object is completed, the gas storage module 10 can be disconnected from the first adsorption module 31 through the first selection module 41 and disconnected from the second adsorption module 32 through the second selection module 42.

[0065] The above embodiments are used to illustrate the parallel adsorption of different target objects (i.e. the adsorption time of different target objects at least partially overlaps on the time axis). In other embodiments, the order of steps four to six can also be adjusted according to the needs to achieve the sequential adsorption of different target objects. For example, the gas storage module 10 can be disconnected from the first adsorption module 31 first, and then another target object can be placed on the second adsorption module 32.

[0066] It can be understood that when the gas storage module 10 delivers the negative pressure to the first adsorption module 31 or the second adsorption module 32, the negative pressure of the gas storage module 10 itself will decrease. Therefore, a gas pressure sensing device 11 can be arranged in the gas storage module 10, and the gas pressure sensing device 11 can be opened all the time or opened in step three / step five, so as to sense the negative pressure value in the gas storage module 10. When the negative pressure value is higher than a preset value, the first power module 21 generates the negative pressure in the gas storage module 10 again.

[0067] Third adsorption method

[0068] As shown in FIG. 4, the first power module 21 and the second power module 22 both need to work in the third adsorption method. The adsorption method is used to adsorb different target objects by the first adsorption module 31 in sequence. It is assumed that the target object is not placed on the first adsorption module 31 in the initial state, and the first adsorption module 31 is communicated with the atmosphere. The third adsorption method can be divided into eight steps, i.e. steps one to eight. According to different needs, the order of the above steps can be changed, and some steps can be omitted or combined.

[0069] Step one, establish the connection between the first power module 21 and the gas storage module 10, the first power module 21 starts and generates negative pressure in the gas storage module 10.

[0070] In some embodiments, when the first power module 21 and the gas storage module 10 are connected through the first pipeline L1 and the gas storage module 10 and the first selection module 41 are connected through the second pipeline L2, this step can also generate negative pressure in the first pipeline L1 and the second pipeline L2.

[0071] Step two, the first power module 21 is closed, and the target object is placed on the first adsorption module 31.

[0072] Step three, establish the connection between the gas storage module 10 and the first adsorption module 31, so that the gas storage module 10 transports negative pressure to the first adsorption module 31, so that the first adsorption module 31 adsorbs the target object by using negative pressure.

[0073] In some embodiments, when the adsorption system 100 includes the first selection module 41, the gas storage module 10 can be communicated with the first adsorption module 31 through the first selection module 41. When the first adsorption module 31 adsorbs the target object by using negative pressure, the target object is fixed on the first adsorption module 31, at which time the corresponding operation can be performed on the target object.

[0074] It can be understood that when the gas storage module 10 transports negative pressure to the first adsorption module 31, the negative pressure of the gas storage module 10 itself will decrease. Therefore, a gas pressure sensing device 11 can be arranged in the gas storage module 10, which can be opened all the time or in step three, so as to sense the negative pressure value in the gas storage module 10, when the negative pressure value is higher than the preset value, the first power module 21 generates negative pressure in the gas storage module 10 again.

[0075] Step four, disconnect the connection between the gas storage module 10 and the first adsorption module 31, so that the negative pressure at the first adsorption module 31 is released.

[0076] In some embodiments, when the corresponding operation on the target object is completed, the gas storage module 10 can be disconnected with the first adsorption module 31 through the first selection module 41.

[0077] Step five, establish the connection between the second power module 22 and the gas storage module 10, the second power module 22 starts and generates negative pressure in the gas storage module 10.

[0078] In some embodiments, when the second power module 22 and the gas storage module 10 are connected through the fourth pipeline L4 and the gas storage module 10 and the second selection module 42 are connected through the fifth pipeline L5, this step can also generate negative pressure in the fourth pipeline L4 and the fifth pipeline L5.

[0079] Step six, the second power module 22 is closed, and another target object is placed on the first adsorption module 31.

[0080] Step seven, the connection between the gas storage module 10 and the first adsorption module 31 is established, and the gas storage module 10 supplies negative pressure to the first adsorption module 31, so that the first adsorption module 31 adsorbs the target object by using the negative pressure. At this time, the target object can be operated accordingly.

[0081] Step eight, the connection between the gas storage module 10 and the first adsorption module 31 is disconnected, so that the negative pressure at the first adsorption module 31 is released.

[0082] The first power module 21 and the second power module 22 are alternately used in the embodiment, which is beneficial to prolong the service life of the first power module 21 and the second power module 22. Even if one of the first power module 21 and the second power module 22 is damaged accidentally, the other one can work as a backup, which is beneficial to the maintenance personnel to maintain the damaged power module during the backup time. In addition, by calculating the pressure building time and the negative pressure drop time corresponding to the time of simultaneously supplying negative pressure to all adsorption modules, the working time of each power module can be designed, so that each power module works alternately to prolong the service life of the power module.

[0083] The adsorption system 100 and the adsorption method of the present application will be further described below according to the specific structure of the adsorption system 100 in combination with specific embodiments. Those skilled in the art should understand that the structure described in the present application is only an embodiment, and any other suitable structure is within the scope of the present application.

[0084] Embodiment 1

[0085] Referring to FIG. 5, the first power module 21 and the second power module 22 respectively adopt the plunger pumps 20a and 20b, the gas storage module 10 adopts the gas tank 10a (with good airtightness, almost no leakage), and the gas pressure sensing device 11 adopts the gas pressure sensor 11a. The first adsorption module 31, the second adsorption module 32, the third adsorption module (not marked in the figure) and the fourth adsorption module (not marked in the figure) respectively adopt the carrier platforms 30a, 30b, 30c and 30d. The first selection module 41, the second selection module 42, the third selection module (not marked in the figure) and the fourth selection module (not marked in the figure) are respectively the three-way electromagnetic valves 40a, 40b, 40c and 40d. The gas tank 10a is connected to the three-way electromagnetic valves 40a, 40b, 40c and 40d through the five-way joint 90. The first sensing device 51, the second sensing device 52, the third sensing device (not marked in the figure) and the fourth sensing device (not marked in the figure) respectively adopt the gas pressure sensors 50a, 50b, 50c and 50d. The first gas-liquid separation device 61, the second gas-liquid separation device 62, the third gas-liquid separation device (not marked in the figure) and the fourth gas-liquid separation device (not marked in the figure) respectively adopt the gas-liquid separators 60a, 60b, 60c and 60d. The first filter device 71, the second filter device 72, the third filter device (not marked in the figure) and the fourth filter device (not marked in the figure) respectively adopt the filters 70a, 70b, 70c and 70d. The target object is the carrier.

[0086] The embodiment can adopt the second adsorption method to adsorb different carrier platforms in sequence, which specifically includes: (1) all the three-way electromagnetic valves 40a, 40b, 40c and 40d are closed, and the plunger pump 20a is opened, so as to establish a negative pressure in the first pipeline L1, the gas tank 10a and the second pipeline L2, and after the negative pressure is established, the plunger pump 20a is closed, and the gas pressure sensor 11a measures the negative pressure value P1; (2) the carrier is placed on the carrier platform 30a, the three-way electromagnetic valve 40a is opened to connect the carrier platform 30a and the gas tank 10a, so that the carrier is adsorbed and fixed to the carrier platform 30a under the action of the negative pressure, and then the fluid can perform biochemical reaction in the carrier; (3) after the biochemical reaction is completed, the three-way electromagnetic valve 40a is closed to connect the carrier platform 30a to the atmosphere, so as to release the negative pressure, at this time the carrier is removed from the carrier platform 30a, and the gas pressure sensor 11a measures the negative pressure change ΔP, that is, the negative pressure in the gas tank 10a is P2=P1+ΔP; (4) repeat the similar steps of (3) to (5) to make other carriers perform biochemical reaction on the carrier platforms 30b, 30c and 30d, and if P2> preset value P0 (preset value), the plunger pump 20a is opened again to make the negative pressure in the gas tank 10a reach P1 again.

[0087] When the plunger pump 20a is started again and a negative pressure is generated in the gas tank 10a, the flow rate of the plunger pump 20a is usually greater than the negative pressure drop, so the plunger pump 20a can complete the pressure building in the gas tank 10a even if the slide glass is held by suction at this time. During the pressure building of the plunger pump 20a, the other plunger pump 20b is used as a backup. In this case, the negative pressures when the four slide glasses (named slide glass a, slide glass b, slide glass c, and slide glass d for convenience) are held by suction in this order are recorded in Table 1.

[0088] Table 1

[0089] In the above step (1), if the pressure building speed of the plunger pump 20a is insufficient, the plunger pump 20a and the plunger pump 20b can be started at the same time to increase the pressure building speed. In another embodiment, the flow rate of the plunger pump 20b can be set to be greater than that of the plunger pump 20a, and the plunger pump 20b can hold a lower negative pressure and the plunger pump 20a can hold a higher negative pressure. In this case, the plunger pump 20b and the plunger pump 20a can be started in this order, the plunger pump 20b is used to quickly build pressure in the fourth pipe L4, the gas tank 10a, and the fifth pipe L5, and then the plunger pump 20a is used to further reduce the negative pressure in the first pipe L1, the gas tank 10a, and the second pipe L2 to the target value, so as to increase the pressure building speed. As shown in FIG. 6, the dotted line part in FIG. 6 represents the degree of the negative pressure drop in the gas tank 10a when the plunger pump 20a is used to build pressure, and the solid line part represents the degree of the negative pressure drop in the gas tank 10a when the plunger pump 20b and the plunger pump 20a are used to build pressure in this order, so it can be seen that the time required for the negative pressure to drop to the target value (indicated by the dashed line) is less when the plunger pump 20b and the plunger pump 20a are used to build pressure in this order, that is, the pressure building speed is higher.

[0090] Referring to FIG. 5, the second adsorption method can also be used to adsorb different slides in parallel, which specifically includes: (1) all three-way electromagnetic valves 40a, 40b, 40c, 40d are closed, and the plunger pump 20a is opened, so that a negative pressure is established in the first pipeline L1, the gas tank 10a and the second pipeline L2, and after the negative pressure is established, the plunger pump 20a is closed, and the gas pressure sensor 11a measures the negative pressure value P1; (2) the slides are placed on the slide platforms 30a, 30b, 30c, 30d respectively, and all three-way electromagnetic valves 40a, 40b, 40c, 40d are opened to respectively connect different slide platforms 30a, 30b, 30c, 30d and the gas tank 10a, so that the slides are adsorbed and fixed to the corresponding slide platforms 30a, 30b, 30c, 30d under the action of negative pressure, and then the fluid can perform biochemical reaction in the slides; (3) after the biochemical reaction is completed, all three-way electromagnetic valves 40a, 40b, 40c, 40d are closed, so that the slide platforms 30a, 30b, 30c, 30d are connected to the atmosphere to release the negative pressure, at this time the slides can be removed from the slide platforms 30a, 30b, 30c, 30d respectively, and the gas pressure sensor 11a measures the negative pressure change 4ΔP, that is, the negative pressure in the gas tank 10a is P2=P1+4ΔP; (4) if P2 is greater than the preset value P0, then the plunger pump 20a is opened again to make the negative pressure in the gas tank 10a reach P1 again.

[0091] The negative pressure conditions of the four slides adsorbed in parallel are recorded in Table 2.

[0092] Table 2

[0093] After the biochemical reaction is completed and the slides are removed from the slide platforms 30a, 30b, 30c, 30d, the slide platforms 30a, 30b, 30c, 30d can also be purged, which specifically includes: (1) all three-way electromagnetic valves 40a, 40b, 40c, 40d are closed, and the plunger pump 20a is opened in the reverse direction, so that a positive pressure is established in the first pipeline L1, the gas tank 10a and the second pipeline L2, and after the positive pressure is established, the plunger pump 20a is closed; (2) the three-way electromagnetic valve 40a is opened to connect the slide platform 30a and the gas tank 10a, and the gas flows from the gas tank 10a to the slide platform 30a and removes the impurities on the slide platform 30a; (3) repeat the similar steps as in (2) to purge other slide platforms 30a, 30b, 30c, 30d. In another embodiment, all three-way electromagnetic valves 40a, 40b, 40c, 40d can also be opened simultaneously in step (2) to purge all slide platforms 30a, 30b, 30c, 30d, and at this time step (3) can be omitted.

[0094] Referring to FIG. 7, in the present embodiment, a shielding member 80 (e.g., a cover plate) can be provided on the slide platform 30a, 30b, 30c or 30d in advance when the slide platform 30a, 30b, 30c or 30d is purged. The shielding member 80 and the slide platform 30a, 30b, 30c or 30d can jointly form an air passage 81 for the positive pressure gas to pass through, which is in communication with the opening H provided on the slide platform 30a.

[0095] Embodiment 2

[0096] Referring to FIG. 8, the difference between the present embodiment and Embodiment 1 is that the gas tank 10a between the first pipeline L1 and the second pipeline L2 is omitted, and only the slide platform 30a is in operation. For simplicity of illustration, the non-operating components are deleted from FIG. 8.

[0097] In the present embodiment, the two plunger pumps 20a, 20b are used to alternately build pressure, which specifically includes: (1) the three-way electromagnetic valve 40a is closed, and the plunger pump 20a is opened, so as to build negative pressure in the first pipeline L1 and the second pipeline L2, and the plunger pump 20a is closed after the negative pressure is built; (2) the slide is placed on the slide platform 30a, and the three-way electromagnetic valve 40a is opened to connect the slide platform 30a with the first pipeline L1 and the second pipeline L2, so that the slide is adsorbed and fixed to the slide platform 30a under the action of the negative pressure, and then the fluid can be subjected to biochemical reaction in the slide; (3) after the biochemical reaction is completed, the three-way electromagnetic valve 40a is closed to connect the slide platform 30a with the atmosphere, so as to release the negative pressure, and the slide is removed from the slide platform 30a at this time; (4) after the second cycle, the other plunger pump 20b is opened, so as to build negative pressure in the fourth pipeline L4 and the second pipeline L2, and the plunger pump 20b is closed after the negative pressure is built; (5) steps (2)-(3) are repeated to perform the second cycle. Among them, it can be switched to step (5) after a long period of time (e.g., half a year or a year), at which time the plunger pump 20b serves as a standby air pump.

[0098] The present embodiment further provides a biochemical substance analysis method. The biochemical substance analysis method first adsorbs a target object (e.g., a slide) on the adsorption module according to the adsorption method described above, and then performs a first operation or a second operation on the slide. The first operation is to make the fluid in the slide undergo biochemical reaction, and the second operation is to detect the fluid in the slide. More specifically, the slide can be a flow cell slide.

[0099] Referring to FIG. 9, the present embodiment further provides a biochemical substance analysis device 1, which includes the adsorption system 100 described above. More specifically, the biochemical substance analysis device 1 can be a gene sequencer.

[0100] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and are not limiting. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A negative pressure adsorption system, characterized in that: include: Gas storage modules; a first power module in communication with the gas storage module and configured to generate a negative pressure within the gas storage module; as well as The first adsorption module is configured to communicate with the gas storage module, and the gas storage module is configured to deliver negative pressure to the first adsorption module, so that the first adsorption module uses the negative pressure to adsorb the target object.

2. The negative pressure adsorption system according to claim 1, wherein: Also includes: A second power module is in communication with the gas storage module and is configured to generate a negative pressure in the gas storage module, wherein at least one of the first power module and the second power module is selectively activated to generate the negative pressure.

3. The negative pressure adsorption system according to claim 2, wherein: The first power module and the second power module are configured to be activated alternately to generate the negative pressure.

4. The negative pressure adsorption system according to claim 2, wherein: The first power module and the second power module are configured to be activated in parallel to generate the negative pressure.

5. The negative pressure adsorption system according to any one of claims 1 to 4, characterized in that: Also includes: The second adsorption module is communicated with the gas storage module, and the gas storage module is further configured to deliver negative pressure to the second adsorption module, so that the second adsorption module uses the negative pressure to adsorb another target object.

6. The negative pressure adsorption system according to any one of claims 1 to 5, characterized in that: Also includes: A first selection module is connected to the gas storage module and the first adsorption module respectively, and the first selection module is configured to connect or disconnect the gas storage module from the first adsorption module.

7. The negative pressure adsorption system according to claim 6, wherein: Also includes: a first pipeline connecting the first power module and the gas storage module; a second pipeline connecting the gas storage module and the first selection module; The third pipeline connects the first selection module and the first adsorption module.

8. The negative pressure adsorption system according to claim 7, wherein: Also includes: a gas pressure sensing device, in communication with the gas storage module and configured to sense a negative pressure value within the gas storage module; The first power module is further configured to generate negative pressure in the gas storage module again when the negative pressure value sensed by the air pressure sensing device is higher than a preset value.

9. The negative pressure adsorption system according to claim 8, wherein: Also includes: The first sensing device is connected to the third pipeline and is configured to sense the negative pressure value in the third pipeline. The negative pressure value sensed by the first power module and the negative pressure value sensed by the first sensing device can indicate whether the adsorption system leaks.

10. The negative pressure adsorption system according to claim 8, wherein: Also includes: The first sensing device is in communication with the third pipe and is configured to sense a gas flow value in the third pipe, where the gas flow value can indicate whether the first adsorption module successfully adsorbs the target object.

11. The negative pressure adsorption system according to any one of claims 7 to 10, characterized in that: Also includes: The gas-liquid separation device is connected to the third pipeline and is configured to block the liquid from flowing from the first adsorption module to the gas storage module.

12. The negative pressure adsorption system according to any one of claims 1 to 11, characterized in that: The adsorption system includes a power module and an adsorption module, the power module includes the first power module and at least one other power module, the adsorption module includes the first adsorption module and at least one other adsorption module, and the number of power modules included in the power module is less than the number of adsorption modules included in the adsorption module.

13. The negative pressure adsorption system according to any one of claims 1 to 12, characterized in that: The first power module is further configured to generate a positive pressure within the gas storage module, and the gas storage module is further configured to deliver the positive pressure to the first adsorption module.

14. A negative pressure adsorption method, characterized in that: include: establishing a connection between the first power module and the gas storage module; The first power module is started and generates negative pressure in the gas storage module; The first power module is turned off and a connection between the gas storage module and the first adsorption module is established; The gas storage module transmits negative pressure to the first adsorption module, so that the first adsorption module adsorbs the target object using the negative pressure.

15. The negative pressure adsorption method according to claim 14, wherein: Also includes: establishing a connection between the second power module and the gas storage module; The second power module is started and generates negative pressure in the gas storage module; The second power module is turned off and a connection between the gas storage module and the second adsorption module is established; The gas storage module transmits negative pressure to the second adsorption module, so that the second adsorption module adsorbs another target object using the negative pressure.

16. The negative pressure adsorption method according to claim 15, wherein: The first power module and the second power module are alternately activated to generate the negative pressure.

17. The negative pressure adsorption method according to claim 15, wherein: The first power module and the second power module are started in parallel to generate the negative pressure.

18. The negative pressure adsorption method according to any one of claims 14 to 17, characterized in that: Also includes: establishing a connection between the gas storage module and a second adsorption module; The gas storage module transmits negative pressure to the second adsorption module, so that the second adsorption module adsorbs another target object using the negative pressure.

19. The negative pressure adsorption method according to any one of claims 14 to 18, wherein: “Establishing a connection between the first power module and the gas storage module” specifically includes: The first selection module is connected to the gas storage module and the first adsorption module respectively, and the first selection module is controlled to connect the gas storage module with the first adsorption module.

20. The negative pressure adsorption method according to any one of claims 14 to 19, characterized in that: Also includes: sensing the negative pressure value in the gas storage module by a gas pressure sensing device; When the negative pressure value is higher than a preset value, the first power module generates negative pressure in the gas storage module again.

21. The negative pressure adsorption method according to any one of claims 14 to 20, characterized in that: Also includes: The first power module generates a positive pressure within the gas storage module; The gas storage module delivers the positive pressure to the first adsorption module.

22. A biochemical substance analysis method, characterized in that: include: establishing a connection between the first power module and the gas storage module; The first power module is started and generates negative pressure in the gas storage module; The first power module is turned off and a connection between the gas storage module and the first adsorption module is established; The gas storage module delivers negative pressure to the first adsorption module, so that the first adsorption module uses the negative pressure to adsorb the wafer; A first operation or a second operation is performed on the slide, wherein the first operation is to cause a biochemical reaction to occur in the fluid in the slide, and the second operation is to detect the fluid in the slide.

23. The biochemical substance analysis method according to claim 22, wherein: Also includes: establishing a connection between the second power module and the gas storage module; The second power module is started and generates negative pressure in the gas storage module; The second power module is shut down and a connection between the gas storage module and the second adsorption module is established; The gas storage module delivers negative pressure to the second adsorption module, so that the second adsorption module uses the negative pressure to adsorb another carrier; A third operation or a fourth operation is performed on the other slide, wherein the third operation is to cause a biochemical reaction to occur in the fluid in the other slide, and the fourth operation is to detect the fluid in the other slide.

24. The biochemical substance analysis method according to claim 23, wherein: The first power module and the second power module are alternately activated to generate the negative pressure.

25. The biochemical substance analysis method according to claim 23, wherein: The first power module and the second power module are started in parallel to generate the negative pressure.

26. The biochemical substance analysis method according to any one of claims 22 to 25, wherein: Also includes: establishing a connection between the gas storage module and a second adsorption module; The gas storage module delivers negative pressure to the second adsorption module, so that the second adsorption module uses the negative pressure to adsorb another carrier; A third operation or a fourth operation is performed on the other slide, wherein the third operation is to cause a biochemical reaction to occur in the fluid in the other slide, and the fourth operation is to detect the fluid in the other slide.

27. The biochemical substance analysis method according to any one of claims 22 to 26, wherein: “Establishing a connection between the first power module and the gas storage module” specifically includes: The first selection module is connected to the gas storage module and the first adsorption module respectively, and the first selection module is controlled to connect the gas storage module with the first adsorption module.

28. The biochemical substance analysis method according to any one of claims 22 to 27, wherein: Also includes: sensing the negative pressure value in the gas storage module by a gas pressure sensing device; When the negative pressure value is higher than the preset value, the first power module generates a pressure in the gas storage module again. Negative pressure.

29. The biochemical substance analysis method according to any one of claims 22 to 28, wherein: Also includes: The first power module generates a positive pressure within the gas storage module; The gas storage module delivers the positive pressure to the first adsorption module, so that the residue of the first adsorption module is removed under the positive pressure.

30. A negative pressure adsorption system, characterized in that: include: a first adsorption module; a first power module; as well as a second power module, at least one of the first power module and the second power module is selectively connected to the first adsorption module, and the first power module and the second power module are configured to generate negative pressure in the first adsorption module so that the first adsorption module uses the negative pressure to adsorb the target object.

31. A biochemical substance analysis device, characterized in that: The method comprises the negative pressure-based adsorption system according to any one of claims 1 to 13 and 30, wherein the adsorption system is configured to adsorb the target object, which is a slide.

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