Agricultural compositions
Agricultural compositions with specific bacterial species and additives enhance drought tolerance in plants, maintaining yields and reducing water usage by improving growth and stress resistance.
Patent Information
- Application Number
- PCT/US2025/025430
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-04-19
- Filing Date
- 2025-04-18
- Publication Date
- 2025-10-23
AI Technical Summary
There is a lack of effective generalist inoculants to enhance drought tolerance in plants such as Solanaceae, Cannabaceae, and Amaryllidaceae, leading to significant yield reductions and water shortages in specialty crop production.
Agricultural compositions comprising microbial populations with specific bacterial species, including Janibacter cremeus, Sphingomonas melonis, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhizobium huakuii, Aeromicrobium ginsengisoli, and Sphingomonas melonis, along with stabilizers, preservatives, dispersants, and wetting agents, applied at concentrations between 1×10^3 to 1×10^12 cells per gram, to improve plant growth and yield under drought conditions.
The compositions maintain and enhance plant yields under drought conditions, reduce water input, and increase marketable yield by improving plant growth and stress tolerance.
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Figure US2025025430_23102025_PF_FP_ABST
Abstract
Description
Attorney Docket No.: IMPE-005 / 02WO 342881-2039 AGRICULTURAL COMPOSITIONS CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Patent Application No. 63 / 636,701, filed April 19, 2024, entitled “MICROBIAL INOCULANTS FOR RESISTANCE TO ABIOTIC AND BIOTIC PLANT STRESSORS,” the disclosure of which is hereby incorporated by reference in its entirety. FIELD OF THE DISCLOSURE
[0002] The present disclosure relates to agricultural compositions for use on plants and plant parts. REFERENCE TO AN ELECTRONIC SEQUENCE LISTING
[0003] The contents of the electronic sequence listing (IMPE_005_02WO_SeqList_ST26.xml; Size: 19,315 bytes; and Date of Creation: April 2, 2025) are herein incorporated by reference in their entirety. BACKGROUND OF THE DISCLOSURE
[0004] According to the 2017 Census of Agriculture, 2,759 farms manage 133,004 irrigated acres of specialty crops in Colorado, 58% of which is dedicated to supporting vegetable production. In the same series of reports, 47 operations discontinued irrigation on a portion of their operations, largely because of a lack of surface or groundwater. More recently, the Department of the Interior declared a water shortage in August of 2021, the subsequent impacts this mandate will have on specialty crops in the coming years will be significant. As an early indication, FSA planting reports associated with specialty crop types have decreased by 15- 78% in Crop Year 21 / 22.
[0005] While the development of drought-resilient specialty crop varieties is a cornerstone of modern breeding efforts, microbial-based soil amendments may provide beneficial traits for host plants under contemporary drought conditions. Plant-microbe interactions in the rhizosphere play a crucial role for plant growth and survival by altering the supply of minerals, plant growth structure and function, and / or disease suppression. While there is information concerning the impacts of drought on the rhizosphere of distinct plants, no research has been done regarding generalist inoculants that could assist in increasing drought tolerance in plants, for example, in Solanaceae (tomatoes, peppers), Cannabaceae (hops, cannabis) and Amaryllidaceous (onions).Attorney Docket No.: IMPE-005 / 02WO 342881-2039 SUMMARY OF THE DISCLOSURE
[0006] The present disclosure relates to methods and compositions to enhance plant tolerance to biotic and abiotic stressors. In some embodiments, the methods and compositions disclosed herein allow plant yields to be maintained during drought conditions, increase yield during under non-stressed conditions, and / or reduce water input.
[0007] In some aspects, the techniques described herein relate to an agricultural composition including: a microbial population that includes at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the microbial population is present in the agricultural composition at between 1×103to 1×1012cells per gram.
[0008] In some aspects, the techniques described herein relate to an agricultural composition including: a microbial population that includes at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity to SEQ ID NO: 6; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the microbial population is present in the agricultural composition at between 1×103to 1×1012cells per gram.
[0009] In some aspects, the techniques described herein relate to a method for improving plant growth and / or increasing marketable yield, the method including: applying to a plant, plant part, or root zone at least one isolated bacterial species having a 16S nucleic acid sequence that shares at least 97% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8, and wherein the application of the at least one isolated bacterial species improves plant growth and / or increases marketable yield of the plant, compared to untreated plants.
[0010] In some aspects, the techniques described herein relate to a microbial composition including: a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 7; a bacterium havingAttorney Docket No.: IMPE-005 / 02WO 342881-2039 a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 8, and an agriculturally acceptable carrier.
[0011] In some aspects, the techniques described herein relate to a microbial composition including: at least one bacterium having a 16S nucleic acid sequence that shares between 97- 99.9% identity with SEQ ID NO: 6; and an agriculturally acceptable carrier. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] The accompanying figures, which are incorporated herein and form a part of the specification, illustrate some, but not the only or exclusive, example embodiments and / or features. It is intended that the embodiments and figures disclosed herein are to be considered illustrative rather than limiting.
[0013] FIG.1 shows the average fruit mass in grams per tomato plant for each treatment group. WW.CK = Adequate-water conditions only (control), WW.Q = Adequate-watering conditions + B1 applied at experimental beginning, SD.CK = Plants under drought only (control), SD.Q.1x = B1 applied 1x at experiment beginning under drought conditions, and SD.Q.WK = B1 applied 1x per week at 3 mL per gallon, under drought conditions.
[0014] FIG.2 shows the average number of green fruits per tomato plant for each treatment group (same as FIG.1).
[0015] FIG.3 shows the average above-ground dry weight biomass (grams) per tomato plant for each treatment group (same as FIG.1).
[0016] FIG. 4 shows a representative image comparing tomato plants across three treatment groups under drought. Left - control plant under drought conditions, middle – one treatment only at the start of drought conditions, and right – one treatment per week under drought conditions.
[0017] FIG. 5 shows total fruit mass and marketable fruit mass for beefsteak tomato variety ‘Defiant 1’ in a drought assay for each of 11 treatment conditions: untreated control (UTC), B4, B6, C6, C7, MB, MB2, MB3, ContinuµmTM(μ), ContinuµmTM+ B4 (μ+4), ContinuµmTM+ B6 (μ+6).
[0018] FIG. 6 shows the average fresh weight in grams per lettuce plant for each treatment group. WWCK = Adequate-watering conditions only (control), WWP (1 mL) = Adequate- watering conditions + 1 mL per gallon B1 at drought-onset, WWP (2 mL) = Adequate-watering conditions + 2 mL per gallon B1 at drought-onset, SDCK = Plants under drought only (control), and SDP = Plants under drought + 1 mL per gallon B1 at drought-onset.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0019] FIG.7 shows the average plant height in centimeters per lettuce plant for each treatment group (same as FIG.6).
[0020] FIG.8 shows the average plant width in centimeters per lettuce plant for each treatment group (same as FIG.6)
[0021] FIG.9 shows a summary of fresh mass, head, greenness, quality, and dry mass results for a drought assay in lettuce variety ‘Ice Queen’ for 11 treatment conditions: (from top to bottom) C1, MB, B8, B7, B6, B5, B4, B3, B2, B1, and untreated control (UTC).
[0022] FIG.10 shows a summary of fresh mass, head formation, greenness, quality, and dry mass results for a drought assay in lettuce variety ‘Crispino’ for 11 treatment conditions: (from top to bottom) ContinuµmTM+ B6 (μ+6), ContinuµmTM+ B4 (μ+4), ContinuµmTM(μ), MB3, MB2, MB, C7, C6, B6, B4, and untreated control (UTC).
[0023] FIG. 11A shows a summary of above ground fresh weight (Avb.FW), head size, maturation, greenness, and quality results in the drought condition for a drought assay in lettuce variety ‘Crispino’ for 7 treatment conditions: (from left to right) untreated control (UTC). B4, B6, C6, MB2, ContinuµmTM(μ), and ContinuµmTM+ B6 (μ+6).
[0024] FIG. 11B shows a summary of the same measurements for the same treatment conditions from FIG.11A under well-watered conditions.
[0025] FIGs.12A and 12B show water usage data (measured as m³ / m³ volume water content (VWC)) for each treatment among bell pepper plants over the course of 60 hours.
[0026] FIG. 13 shows representative images of bell pepper plants across treatment groups under drought. The top and middle images correspond to untreated control (top left), MB (top right), C1 (middle left), and B6 (middle right) treatment conditions. The bottom two images show a birdseye view (bottom left) and a front view (bottom right) for untreated control (left) and MC1 (right) treatment conditions.
[0027] FIG. 14 shows average number of fruits per plant and average fruit quality results in bell pepper variety ‘Green Machine’ in a drought assay with 12 treatment conditions: (from left to right) untreated control (UTC), PosCk, C1, B1, B2, B3, B4, B5, B6, B7, B8, and MB.
[0028] FIG.15 shows average fruit mass and market mass results in bell pepper variety ‘Green Machine’ in a drought assay with 11 treatment conditions: (from left to right) untreated control (UTC), B4, B6, C6, C7, MB, MB2, MB3, ContinuµmTM(μ), ContinuµmTM+ B4 (μ+4), and ContinuµmTM+ B6 (μ+6).Attorney Docket No.: IMPE-005 / 02WO 342881-2039 DETAILED DESCRIPTION OF THE DISCLOSURE
[0029] While various embodiments of the disclosure have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions may occur to those skilled in the art without departing from the disclosure. It should be understood that various alternatives to the embodiments of the disclosure described herein may be employed. Definitions
[0030] The use of the terms “a” and “an” and “the” and similar referents in the context of describing the disclosure (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms “comprising,” “having,” “including,” and “containing” are to be construed as open-ended terms (i.e., meaning “including, but not limited to,”) unless otherwise noted. Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, including the end points, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. For example, if the range 10-15 is disclosed, then 10, 11, 12, 13, 14, and 15 are also disclosed. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as") provided herein, is intended merely to better illuminate the disclosure and does not pose a limitation on the scope of the disclosure unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the disclosure.
[0031] As used herein, the term “about” is used synonymously with the term “approximately.” Illustratively, the use of the term “about” with regard to an amount indicates that values slightly outside the cited values, e.g., plus or minus 0.1% to 10%.
[0032] As used herein, “in planta” may refer to in the plant, on the plant, or intimately associated with the plant, depending upon context of usage (e.g. endophytic, epiphytic, or rhizospheric associations). The plant may comprise plant parts, tissue, leaves, roots, root hairs, rhizomes, stems, seed, ovules, pollen, flowers, fruit, etc.
[0033] As used herein the term “plant part” can include tissue, leaves, roots, root hairs, rhizomes, stems, seeds, ovules, pollen, flowers, fruit, etc.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0034] As used herein the terms “microorganism” or “microbe” should be taken broadly. These terms, used interchangeably, include but are not limited to, the two prokaryotic domains, Bacteria and Archaea. The term may also encompass eukaryotic fungi and protists.
[0035] “Drought stress” as used herein is induced in plants under conditions where reduced water content in the soil, due to, e.g., a shortage of rainfall or irrigation, leads to impaired or reduced water absorption by the plant.
[0036] As used herein, the “root zone” refers to the entire volume of soil that roots are growing in, coming into contact with, and / or effectively utilizing. The root zone includes the rhizosphere.
[0037] As used herein, the “rhizosphere” is a region within the root zone that is directly influenced by root secretions and associated with soil microorganisms known as the root microbiome.
[0038] As used herein, “isolate,” “isolated,” “isolated microbe,” “isolated bacteria,” and like terms, are intended to mean that the one or more microorganisms has been separated from at least one of the materials with which it is associated in a particular environment (for example soil, water, plant tissue, etc.). Thus, an “isolated microbe” does not exist in its naturally occurring environment; rather, it is through the various techniques described herein that the microbe has been removed from its natural setting and placed into a non-naturally occurring state of existence. Thus, the isolated strain or isolated microbe may exist as, for example, a biologically pure culture, or as spores (or other forms of the strain). In aspects, the isolated microbe may be in association with an acceptable carrier, which may be an agriculturally acceptable carrier.
[0039] In certain aspects of the disclosure, the isolated microbes exist as isolated and biologically pure cultures. It will be appreciated by one of skill in the art, that an isolated and biologically pure culture of a particular microbe, denotes that said culture is substantially free (within scientific reason) of other living organisms and contains only the individual microbe in question. The culture can contain varying concentrations of said microbe. The present disclosure notes that isolated and biologically pure microbes often “necessarily differ from less pure or impure materials.” See, e.g. In re Bergstrom, 427 F.2d 1394, (CCPA 1970)(discussing purified prostaglandins), see also, In re Bergy, 596 F.2d 952 (CCPA 1979)(discussing purified microbes), see also, Parke-Davis & Co. v. H.K. Mulford & Co., 189 F. 95 (S.D.N.Y. 1911) (Learned Hand discussing purified adrenaline), aff’d in part, rev’d in part, 196 F.496 (2d Cir. 1912), each of which are incorporated herein by reference. Furthermore, in some aspects, the disclosure provides for certain quantitative measures of the concentration, or purity limitations,Attorney Docket No.: IMPE-005 / 02WO 342881-2039 that must be found within an isolated and biologically pure microbial culture. The presence of these purity values, in certain embodiments, is a further attribute that distinguishes the presently disclosed microbes from those microbes existing in a natural state. See, e.g., Merck & Co. v. Olin Mathieson Chemical Corp., 253 F.2d 156 (4th Cir. 1958) (discussing purity limitations for vitamin B12 produced by microbes), incorporated herein by reference.
[0040] As used herein, “individual isolates” should be taken to mean a composition, or culture, comprising a predominance of a single genera, species, or strain, of microorganism, following separation from one or more other microorganisms. The phrase should not be taken to indicate the extent to which the microorganism has been isolated or purified. However, “individual isolates” can comprise substantially only one genus, species, or strain, of microorganism.
[0041] As used herein, “sequence identity" or "identity" in the context of two nucleic acid or polypeptide sequences includes reference to the number of residues in the two sequences which are the same when aligned for maximum correspondence over a specified comparison window. When percentage of sequence identity is used in reference to proteins it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity) and therefore do not change the functional properties of the molecule. Where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences which differ by such conservative substitutions are said to have "sequence similarity" or "similarity." Means for making this adjustment are well-known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, e.g., according to the algorithm of Meyers and Miller, Computer Applic. Biol. Sci., 4:11-17 (1988). The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm. An example of a local alignment algorithm utilized for the comparison of sequences is the NCBI Basic Local Alignment Search Tool (BLAST®) (Altschul et al. 1990 J. Mol. Biol. 215: 403-10), which is available from several sources, including the National Center for Biotechnology Information (NCBI, Bethesda, Md.) and on the Internet, for use in connection with the sequence analysis programs blastp, blastn, blastx, tblastn and tblastx. It can be accessed on the internet via the National Library of MedicineAttorney Docket No.: IMPE-005 / 02WO 342881-2039 (NLM)'s world-wide-web URL. A description of how to determine sequence identity using this program is available at the NLM's website on BLAST tutorial. Another example of a mathematical algorithm utilized for the global comparison of sequences is the Clustal W and Clustal X (Larkin et al.2007 Bioinformatics, 23, 2947-294, Clustal W and Clustal X version 2.0) as well as Clustal omega. Unless otherwise stated, references to sequence identity used herein refer to the NCBI Basic Local Alignment Search Tool (BLAST®).
[0042] As used herein, the phrase “synthetic composition” refers to a composition that includes at least one microbe that has been isolated, cultured, enriched, and / or genetically engineered.
[0043] The term “microbial consortia” or “microbial consortium” refers to a composition comprising two or more distinct microbial species, or strains of species, that exhibit cooperative, synergistic, or complementary metabolic, biochemical, or ecological functions. The microbial consortia described herein are synthetically assembled and / or selectively enriched to enhance performance in an intended application, including but not limited to agricultural contexts.
[0044] The term “marketable yield” refers to the amount of salable produce from a crop, considering both quantity (number and / or weight) and quality factors such as size, shape, color, and uniformity. It is the portion of a crop that can be sold on the market. Overview
[0045] The present disclosure provides agricultural compositions comprising at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8, and combinations thereof, and methods of using the same to improve or maintain marketable traits in plants and commodity plant products, such as fruits and vegetables, under normal conditions as well as under abiotic stressors. Microbial Isolates and Consortia
[0046] The present disclosure, in certain embodiments, provides isolated and biologically pure microorganisms that have applications, inter alia, in agriculture. The disclosed microorganisms can be utilized in their isolated and biologically pure states, as well as being formulated into compositions (see below section for exemplary composition descriptions) which may comprises metabolites produced by the disclosed microorganisms. Furthermore, the disclosure provides microbial compositions containing at least two members of the disclosed isolated and biologically pure microorganisms, as well as methods of utilizing said microbial compositions.
[0047] In some embodiments, the disclosure provides an agricultural composition comprising:Attorney Docket No.: IMPE-005 / 02WO 342881-2039 a microbial population that comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier. In some aspects, the at least one bacterium is present in the agricultural composition at between 1×103to 1×1012bacterial cells per gram.
[0048] In some embodiments, the disclosure provides an agricultural composition comprising: at least one isolated bacterial species selected from the group consisting of Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhizobium huakuii, Aeromicrobium ginsengisoli, Sphingomonas melonis, and combinations thereof, wherein each of the isolated bacterial species is comprised within a microbial consortium deposited as NCMA No. 202504005; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the at least one isolated bacterial species or combination thereof is present in the agricultural composition at between 1×103to 1×1012bacterial cells per gram.
[0049] In aspects, the disclosure provides agricultural compositions comprising at least one isolated bacterial species selected from amongst the microbes identified in Table 1, or combinations thereof. Table 1: Bacterial Isolates and Consortia Genus Species 16S rRNA SEQ ID NO Janibacter cremeus SEQ ID NO: 1 Sphingomonas melonis SEQ ID NO: 2 Mesorhizobium ciceri SEQ ID NO: 3 Pseudomonas thivervalensisSEQ ID NO: 4Sphingomonas oligoaromativoransSEQ ID NO: 5Mesorhizobium huakuiiSEQ ID NO: 6Aeromicrobium ginsengisoliSEQ ID NO: 7Sphingomonas melonisSEQ ID NO: 8
[0050] In certain embodiments, the consortia of the present disclosure comprise two microbes, or three microbes, or four microbes, or five microbes, or six microbes, or seven microbes, or eight microbes, or nine or more microbes. Said microbes of the consortia are different microbial species, or different strains of a microbial species.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0051] In particular aspects, the disclosure provides bacterial consortium, comprising species as grouped in Tables 2-8. With respect to Tables 2-8, the letter I represents a non-limiting additional microbe(s) which may be present in the agricultural compositions of the present disclosure. A = Janibacter cremeus and associated strains or related species; B = Sphingomonas mali and associated strains or related species; C = Mesorhizobium ciceri and associated strains or related species; D = Pseudomonas thivervalensis and associated strains or related species; E = Sphingomonas oloaromativorans and associated strains or related species; F = Mesorhizobium huakuii and associated strains or related species; G = Aeromicrobium ginsengisoli and associated strains or related species; H = Sphingomonas melonis and associated strains or related species; and I = other plant beneficial microbes. Table 2: Eight and Nine Strain Consortia A,B,C,D,E,F,G,H A,B,C,D,E,F,G,I A,B,C,D,E,F,H,I A,B,C,D,E,G,H,I A,B,C,D,F,G,H,I A,B,C,E,F,G,H,I A,B,D,E,F,G,H,I A,C,D,E,F,G,H,I B,C,D,E,F,G,H,I A,B,C,D,E,F,G,H,ITable 3: Seven Strain Consortia A,B,C,D,E,F,G A,B,C,D,E,F,H A,B,C,D,E,F,I A,B,C,D,E,G,H A,B,C,D,E,G,I A,B,C,D,E,H,I A,B,C,D,F,G,H A,B,C,D,F,G,I A,B,C,D,F,H,I A,B,C,D,G,H,I A,B,C,E,F,G,H A,B,C,E,F,G,I A,B,C,E,F,H,I A,B,C,E,G,H,I A,B,C,F,G,H,I A,B,D,E,F,G,H A,B,D,E,F,G,I A,B,D,E,F,H,I A,B,D,E,G,H,I A,B,D,F,G,H,I A,B,E,F,G,H,I A,C,D,E,F,G,H A,C,D,E,F,G,I A,C,D,E,F,H,I A,C,D,E,G,H,I A,C,D,F,G,H,I A,C,E,F,G,H,I A,D,E,F,G,H,I B,C,D,E,F,G,H B,C,D,E,F,G,I B,C,D,E,F,H,I B,C,D,E,G,H,I B,C,D,F,G,H,I B,C,E,F,G,H,I B,D,E,F,G,H,I C,D,E,F,G,H,I Table 4: Six Strain Consortia A,B,C,D,E,F A,B,C,D,E,G A,B,C,D,E,H A,B,C,D,E,I A,B,C,D,F,G A,B,C,D,F,H A,B,C,D,F,I A,B,C,D,G,H A,B,C,D,G,I A,B,C,D,H,I A,B,C,E,F,G A,B,C,E,F,H A,B,C,E,F,I A,B,C,E,G,H A,B,C,E,G,I A,B,C,E,H,I A,B,C,F,G,H A,B,C,F,G,I A,B,C,F,H,I A,B,C,G,H,I A,B,D,E,F,G A,B,D,E,F,H A,B,D,E,F,I A,B,D,E,G,H A,B,D,E,G,I A,B,D,E,H,I A,B,D,F,G,H A,B,D,F,G,I D,E,F,G,H,I C,E,F,G,H,I A,B,D,F,H,I A,B,D,G,H,I A,B,E,F,G,H A,B,E,F,G,I A,B,E,F,H,I A,B,E,G,H,I A,B,F,G,H,I A,C,D,E,F,G A,C,D,E,F,H A,C,D,E,F,I A,C,D,E,G,H A,C,D,E,G,I A,C,D,E,H,I A,C,D,F,G,H A,C,D,F,G,I A,C,D,F,H,I A,C,D,G,H,I A,C,E,F,G,H A,C,E,F,G,I A,C,E,F,H,I A,C,E,G,H,I A,C,F,G,H,I A,D,E,F,G,H A,D,E,F,G,I A,D,E,F,H,I A,D,E,G,H,I A,D,F,G,H,I A,E,F,G,H,I B,C,D,E,F,G B,C,D,E,F,H B,C,D,E,F,I B,C,D,E,G,H B,C,D,E,G,I B,C,D,E,H,I B,C,D,F,G,H B,C,D,F,G,I B,C,D,F,H,I B,C,D,G,H,I B,C,E,F,G,H B,C,E,F,G,I B,C,E,F,H,I B,C,E,G,H,I B,C,F,G,H,I B,D,E,F,G,H B,D,E,F,G,I B,D,E,F,H,I B,D,E,G,H,I B,D,F,G,H,I B,E,F,G,H,I C,D,E,F,G,H C,D,E,F,G,I C,D,E,F,H,I C,D,E,G,H,I C,D,F,G,H,IAttorney Docket No.: IMPE-005 / 02WO 342881-2039 Table 5: Five Strain Consortia A,B,C,D,E A,B,C,D,F A,B,C,D,G A,B,C,D,H A,B,C,D,I A,B,C,E,F A,B,C,E,G A,B,C,E,H A,B,C,F,H A,B,C,F,G A,B,C,F,I A,B,C,G,H A,B,C,G,I A,B,C,H,I A,B,D,E,F A,B,D,E,G A,B,D,E,I A,B,D,F,G A,B,D,F,H A,B,D,F,I A,B,D,G,H A,B,D,G,I A,B,D,H,I A,B,E,F,G A,B,E,F,I A,B,E,G,H A,B,E,G,I A,B,E,H,I A,B,F,G,H A,B,F,G,I A,B,F,H,I A,B,G,H,I A,C,D,E,G A,C,D,E,H A,C,D,E,I A,C,D,F,G A,C,D,F,H A,C,D,F,I A,C,D,G,H A,C,D,G,I A,C,E,F,G A,C,E,F,H A,C,E,F,I A,C,E,G,H A,C,E,G,I A,C,E,H,I A,C,F,G,H A,C,F,G,I A,C,G,H,I A,D,E,F,G A,D,E,F,H A,D,E,F,I A,D,E,G,H A,D,E,G,I A,D,E,H,I A,D,F,G,H A,D,F,H,I A,D,G,H,I A,E,F,G,H A,E,F,G,I A,E,F,H,I A,E,G,H,I A,F,G,H,I B,C,D,E,F B,C,D,E,H B,C,D,E,I B,C,D,F,G B,C,D,F,H B,C,D,F,I B,C,D,G,H B,C,D,G,I B,C,D,H,I B,C,E,F,H B,C,E,F,I B,C,E,G,H B,C,E,G,I B,C,E,H,I B,C,F,G,H B,C,F,G,I B,C,F,H,I B,D,E,F,G B,D,E,F,H B,D,E,F,I B,D,E,G,H B,D,E,G,I B,D,E,H,I B,D,F,G,H B,D,F,G,I B,D,G,H,I B,E,F,G,H B,E,F,G,I B,E,F,H,I B,E,G,H,I B,F,G,H,I C,D,E,F,G C,D,E,F,H C,D,E,G,H C,D,E,G,I C,D,E,H,I C,D,F,G,H C,D,F,G,I C,D,F,H,I C,D,G,H,I C,E,F,G,H C,E,F,H,I C,E,G,H,I C,F,G,H,I D,E,F,G,H D,E,F,G,I D,E,F,H,I D,E,G,H,I D,F,G,H,I A,B,C,E,I A,B,D,E,H A,B,E,F,H A,C,D,E,F A,C,D,H,I A,C,F,H,I A,D,F,G,I B,C,D,E,G B,C,E,F,G B,C,G,H,I B,D,F,H,I C,D,E,F,I C,E,F,G,I E,F,G,H,ITable 6: Four Strain Consortia A,B,C,D A,B,C,E A,B,C,F A,B,C,G A,B,C,H A,B,C,I A,B,D,E A,B,D,F D,G,H,I A,B,D,G A,B,D,H A,B,D,I A,B,E,F A,B,E,G A,B,E,H A,B,E,I A,B,F,G E,F,G,H A,B,F,H A,D,F,H A,D,F,I A,D,G,H A,D,G,I A,D,H,I A,E,F,G A,E,F,H E,F,G,I A,B,F,I A,B,G,H A,B,G,I A,B,H,I A,C,D,E A,C,D,F A,C,D,G A,C,D,H E,F,H,I A,C,D,I A,C,E,F A,C,E,G A,C,E,H A,C,E,I A,C,F,G A,C,F,H A,C,F,I E,G,H,I A,C,G,H A,C,G,I A,C,H,I A,D,E,F A,D,E,G A,D,E,H A,D,E,I A,D,F,G F,G,H,I A,E,F,I A,E,G,H A,E,G,I A,E,H,I A,F,G,H A,F,G,I A,F,H,I A,G,H,I D,E,F,H B,C,D,E B,C,D,F B,C,D,G B,C,D,H B,C,D,I B,C,E,F B,C,E,G B,C,E,H D,E,F,I B,C,E,I B,C,F,G B,C,F,H B,C,F,I B,C,G,H B,C,G,I B,C,H,I B,D,E,F D,E,G,H B,D,E,G B,D,E,H B,D,E,I B,D,F,G B,D,F,H B,D,F,I B,D,G,H B,D,G,I D,E,G,I B,D,H,I B,E,F,G B,E,F,H B,E,F,I B,E,G,H B,E,G,I B,E,H,I B,F,G,H D,E,H,I B,F,G,I B,F,H,I B,G,H,I C,D,E,F C,D,E,G C,D,E,H C,D,E,I C,D,F,G D,F,G,H C,D,F,H C,D,F,I C,D,G,H C,D,G,I C,D,H,I C,E,F,G C,E,F,H C,E,F,I D,F,G,I C,E,G,H C,E,G,I C,E,H,I C,F,G,H C,F,G,I C,F,H,I C,G,H,I D,E,F,G D,F,H,I Table 7: Three Strain Consortia A,B,C A,B,D A,B,E A,B,F A,B,G A,B,H A,B,I A,C,D A,C,E G,H,I E,F,H A,C,F A,C,G A,C,H A,C,I A,D,E A,D,F A,D,G A,D,H A,D,I F,H,I E,F,G A,E,F A,E,G A,E,H A,E,I A,F,G A,F,H A,F,I A,G,H A,G,I F,G,I D,H,I A,H,I B,C,D B,C,E B,C,F B,C,G B,C,H B,C,I B,D,E B,D,F F,G,H D,G,I B,D,G B,D,H B,D,I B,E,F B,E,G B,E,H B,E,I B,F,G B,F,H E,H,I E,F,I B,F,I B,G,H B,G,I B,H,I C,D,E C,D,F C,D,G C,D,H C,D,I E,G,I D,G,H C,E,F C,E,G C,E,H C,E,I C,F,G C,F,H C,F,I C,G,H C,G,I E,G,H D,F,I C,H,I D,E,F D,E,G D,E,H D,E,I D,F,G D,F,HAttorney Docket No.: IMPE-005 / 02WO 342881-2039 Table 8: Two Strain Consortia A,B A,C A,D A,E A,F A,G A,H A,I B,C B,D B,E B,F B,G B,H B,I C,D C,E C,F C,G C,H C,I D,E D,F D,G D,H D,I E,F E,G E,H E,I F,G F,H F,I G,H G,I H,I
[0052] In some embodiments, the microbial consortia may be selected from any member group from Tables 2-8.
[0053] In some embodiments, the disclosure provides an agricultural composition comprising: a microbial population that comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the microbial population is present in the agricultural composition at between 1×103to 1×1012cells per gram.
[0054] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8. In some aspects, the microbial population comprises at least one bacterium with a 16S nucleic acid selected from the group consisting of SEQ ID NOs: 1- 8.
[0055] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 1. In some aspects, the at least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 1.
[0056] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 1. In some aspects, the at least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 2.
[0057] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%,Attorney Docket No.: IMPE-005 / 02WO 342881-2039 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 1. In some aspects, the at least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 3.
[0058] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 1. In some aspects, the at least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 4.
[0059] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 1. In some aspects, the at least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 5.
[0060] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 1. In some aspects, the at least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 6.
[0061] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 1. In some aspects, the at least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 7.
[0062] In some embodiments, the microbial population of the agricultural composition comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 1. In some aspects, the at least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 8.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0063] In some aspects, the microbial population of the agricultural composition comprises Janibacter cremeus as deposited at NCMA 202504005.
[0064] In some aspects, the microbial population of the agricultural composition comprises Sphingomonas mali as deposited at NCMA 202504005.
[0065] In some aspects, the microbial population of the agricultural composition comprises Mesorhizobium ciceri as deposited at NCMA 202504005.
[0066] In some aspects, the microbial population of the agricultural composition comprises Pseudomonas thivervalensis as deposited at NCMA 202504005.
[0067] In some aspects, the microbial population of the agricultural composition comprises Sphingomonas oligoaromativorans as deposited at NCMA 202504005.
[0068] In some aspects, the microbial population of the agricultural composition comprises Mesorhizobium huakuii as deposited at NCMA 202504004.
[0069] In some aspects, the microbial population of the agricultural composition comprises Aeromicrobium ginsengisoli as deposited at NCMA 202504005.
[0070] In some aspects, the microbial population of the agricultural composition comprises Sphingomonas melonis as deposited at NCMA 202504005.
[0071] In some aspects, the microbial population of the agricultural composition comprises two isolated bacterial species. In some aspects, the microbial population comprises between three and five isolated bacterial species. In some aspects, the microbial population comprises between five and seven isolated bacterial species. In some aspects, the microbial population comprises seven or eight isolated bacterial species.
[0072] In some aspects, the at least one bacterium comprises isolated bacterial species Janibacter cremeus, and Sphingomonas mali, said isolated bacterial species deposited at NCMA 202504005.
[0073] In some aspects, the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, and Pseudomonas thivervalensis, said isolated bacterial species deposited at NCMA 202504005.
[0074] In some aspects, the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005.
[0075] In some aspects, the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Aeromicrobium ginsengisoli, said isolated bacterial species deposited at NCMA 202504005.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0076] In some aspects, the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, Mesorhizobium huakuii, and Aeromicrobium ginsengisoli, said isolated bacterial species deposited at NCMA 202504005.
[0077] In some aspects, the at least one bacterium comprises isolated bacterial species Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005.
[0078] In some aspects, the at least one bacterium comprises isolated bacterial species Pseudomonas thivervalensis and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005.
[0079] In some aspects, the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, Aeromicrobium ginsengisoli, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005.
[0080] In some aspects, the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005.
[0081] In some aspects, the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Mesorhozobium huakii, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005.
[0082] In some aspects, each isolated bacterial species is present in the composition at approximately equal ratios. In some aspects, the agricultural composition is formulated with unequal ratios of bacterial species to accommodate the needs of a particular crop.
[0083] In some embodiments, the disclosure provides an agricultural composition comprising: a microbial population that comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity to SEQ ID NO: 6; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the microbial population is present in the agricultural composition at between 1×103to 1×1012cells per gram. In some aspects, the at least one bacterium has a 16S nucleic acid sequences that shares at least 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98.0%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%. 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, or 99.9% identity with SEQ ID NO: 6. In some aspects, the atAttorney Docket No.: IMPE-005 / 02WO 342881-2039 least one bacterium with a 16S nucleic acid sequence comprises SEQ ID NO: 6. In some aspects, the at least one bacterium is Mesorhizobium huakuii as deposited at NCMA 202504004. In some aspects, the microbial population further comprises a bacterium with a 16S nucleic acid sequence that shares at least 97% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-5 and 7-8.
[0084] In some embodiments, the agricultural compositions of the present disclosure comprise between 1×104to 1×1012cells per gram, between 1×106to 1×1012cells per gram, or between 1×108to 1×1012cells per gram.
[0085] In some embodiments, the agricultural compositions of the present disclosure is a liquid, and further comprises at least one metabolite produced by the at least one bacterium. In some aspects, the at least one metabolite is selected from the group consisting of: bacteriocins, lipopeptides, polyketides, siderophores, enzymes, auxins, giberellins, cytokinins, pyrazines, indole, sulpher-containing compounds, organic acids, alcohols, and exopolysaccharides.
[0086] In some embodiments, the disclosure relates to a synthetic composition comprising a plant or a plant part, and the agricultural composition described herein. In some aspects, the synthetic combination of the plant or plant part and the agricultural compositions described herein are where the at least one bacterium is coated onto a seed, or applied onto the surface of a part of the plant, or applied to an area into which a plant will be planted, or combined with a fertilizer or other agricultural composition. Abiotic stressors
[0087] The methods and compositions of the present disclosure may increase plant survivability under a number of abiotic stressors. Examples of abiotic stressors include heat stress, salt (salinity) stress, drought stress, cold stress, excess water stress, and low nutrient stress. Plant Beneficial Microbes & Biologicals
[0088] In some embodiments, the agricultural compositions of the present disclosure further comprise plant beneficial microbes, for example, biologicals. Biologicals are products that are created from, or derived from, living organisms, plant extracts, beneficial insects, or other organic matter. Additional names in the art include, for example, bioeffector, biocontrol agent, bioherbicide, bioactivity, biorational insecticide, and biodigester.
[0089] In some embodiments, a biological comprised by a composition herein, or employed in a method herein, comprises a biostimulant, a biopesticide, or a biofertilizer. In some embodiments, a biological herein is a composition comprising microbes, e.g., plant beneficialAttorney Docket No.: IMPE-005 / 02WO 342881-2039 microbes. In some embodiments, a biological herein comprises a rhizobacterium. In some embodiments, the biological comprises a Bacillus sp. In some embodiments, the biological comprises Azospirillum sp. In some embodiments, the biological comprises Pantoea sp. In some embodiments, a biological herein comprises an Agrobiotech product from Probelte®. In some embodiments, a biological herein comprises a product from Impello®.
[0090] In some embodiments, a biological herein is a composition comprising microbes. In some embodiments, a biological herein is a product listed in Table 10. In some embodiments, a biological herein comprises a bacterial strain listed in Table 10. Table 10: Illustrative biological products and bacterial strains Product Strains Impello® Tribus® Bacillus subtilis strain Itri1 Bacillus pumilus strain Itri2 Bacillus amyloliquefaciens strain Itri3 Impello® Continuum™ Paenibacillus chitinolyticus strain Icon1, Bacillus subtilis strain Icon2 Bacillus pumilus strain Icon3 Bacillus amyloliquefaciens strain Icon4 Probelte® Biopron™ or Azospirillum brasilense strain Pbio1 Biopron™ Premium Pantoea dispersa strain Pbio2 Probelte® Bøtrybël™ Bacillus amyloliquefaciens strain Pbot1 Probelte® Bulhnova™ Azospirillum brasilense strain Pbul1 Pantoea dispersa strain Pbul2 Probelte® Nemapron™ Azospirillum sp. strain Pnem1 Bacillus spp. strain Pnem2 Probelte® Strongest™ Azospirillum brasilense strain Pstr1 Probelte® Belthirul™ Bacillus thuringiensis var. kurstaki strain Pbel1 Probelte® Belthirul™ F Bacillus thuringiensis var. kurstaki strain Pbel1F Probelte® Belthirul™ S Bacillus thuringiensis var. kurstaki strain Pbel1S Probelte® Belthirul™ 16 SC Bacillus thuringiensis var. kurstaki strain Pbel116SC Probelte® Lepiback™ Bacillus thuringiensis var. kurstaki strain Plep1
[0091] In some embodiments, a biological herein comprises any of the species or strains listed in Table 1. In some embodiments, the biological comprises about 103, 104, 105, 106, 107, 108,Attorney Docket No.: IMPE-005 / 02WO 342881-2039 109, 1010, 1011, 1012, CFU / mL of a microbe listed herein. In some embodiments, the biological comprises about 107, 108, 109, or 1010CFU / g of a microbe listed herein. Biostimulants
[0092] In some embodiments, the biological comprises a biostimulant. Biostimulants are substances or microorganisms that, when applied to seeds, plants, or the rhizosphere, stimulate natural processes within the plant or the plant microbiome (including the entirety of the phytomicrombiome, e.g. the phyllosphere and rhizosphere) to enhance or benefit nutrient uptake, nutrient efficiency, tolerance to abiotic stress, or crop quality and yield. In some embodiments, the biological is a biostimulant. In some embodiments, the biostimulant comprises humic substances, hormones, cell signaling molecules, seaweed extract, and / or amino acids.
[0093] In some embodiments, the biological is selected from auxins, cytokinins, gibberellins, abscisic acid, ethylene, brassinosteroids, jasmonic acid, strigolactones, chemical mimics of strigolactone, and combinations thereof.
[0094] In some embodiments, the biostimulant comprises a strigolactone or chemical mimics of strigolactone. Such compounds are described in PCT / US2016 / 029080, filed April 23, 2016, and entitled: Methods for Hydraulic Enhancement of Crops, and US2021 / 0329917, published October 28, 2021 and entitled: Compounds and Methods for Increasing Soil Nutrient Availability, which are hereby incorporated by reference. They are further described in U.S. Patent No. 9,994,557, issued on June 12, 2018, and entitled: Strigolactone Formulations and Uses Thereof, which is hereby incorporated by reference. Biofertilizers
[0095] In some embodiments, the agricultural compositions of the present disclosure further comprise a biofertilizer. Biofertilizers are microorganisms, such as bacteria, fungi, and algae, that provide plants with nutrients, or help them to absorb nutrients, thus improving plant yield. Types of biofertilizers include, but are not limited to, microbes that increase nitrogen fixation, microbes that increase phosphate solubilization, microbes that increase nutrient mobilization, plant growth-promoting microbes, and plant growth-regulating microbes.
[0096] In some embodiments, the biological is a biofertilizer selected from the group consisting of a bacterial, algal, and fungal biofertilizer. In some embodiments, the biological is a biofertilizer that comprises at least one of a nitrogen fixer, a phosphate solubilizer, a nutrient mobilizer, plant growth-promoting bacteria, and plant growth-regulating bacteria.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0097] In some embodiments, the biological comprises one or more species of cultured microbe selected from Methylobacterium, mycorrhizal fungi, Gluconacetobacter, Achromobacter, Agrobacterium, Anabaena, Azorhizobium, Azospirillum, Azotobacter, Bacillus, Beauveria, Bradyrhizobium, Clostridium, Enterobacter, Klebsiella, Kluyvera, Kosakonia, Mesorhizobium, Microbacterium, Ochrobactrum, Pantoea, Penicillium, Pseudomonas, Rahnella, Rhizoctonia, Rhizobium, Rhodopseudomonas, Sinorhizobium, Trichoderma, and combinations thereof.
[0098] In some embodiments, the biological comprises plant growth-promoting fungi and / or plant growth-promoting bacteria. Probelte® biologicals
[0099] In some embodiments, the agricultural compositions of the present disclosure further comprise a biological from Probelte®. In some embodiments, the biological is an Agrobiotech product from Probelte®. In some embodiments, the biological is Biopron™ Premium, Bøtrybël™, Bulhnova™, Nemapron™, Strongest™, Belthirul™, Belthirul™ F, Belthirul™ S, Belthirul™ 16 SC, or Lepiback™. In some embodiments, the biological is a product listed in the product catalog of Probelte®, which can be retrieved from the world wide web at: probelte.com / wp-content / uploads / 2022 / 02 / Probelte_Product_catalogue.pdf. In some embodiments, the biological is Bøtrybël™. In some embodiments, the biological comprises Bacillus amyloliquefaciens. In some embodiments, the biological comprises 108CFU / mL Bacillus amyloliquefaciens. In some embodiments, the biological is for administration at a dose of 12-15 cc / L as a foliar application every 7-14 days. In some embodiments, the biological is for administration at 5-15 L / ha, 1-5 times. For example, in some embodiments, the biological is for administration 5-7 days after transplanting, 30-40 days after first application, and 50-60 days after first application.
[0100] In some embodiments, the biological is a Probelte® product, and it is administered in conjunction with a composition disclosed herein, and it is for administration at the recommended dose according to its product packaging or labeling. In some embodiments, the Probelte® product is administered separately from a composition disclosed herein. In some embodiments, the Probelte® product is administered in combination with a composition disclosed herein.
[0101] In some embodiments, the biological is a composition or microbial strain disclosed in any one of WO-2008113873-A1, WO-2009013596-A2, WO-2009031023-A2, WO- 2011036316-A2, WO-2011121408-A1, WO-2008090460-A1, WO-2009027544-A1, WO-Attorney Docket No.: IMPE-005 / 02WO 342881-2039 2010041096-A1, or WO-2020216978-A1, each of which is incorporated by reference herein in its entirety. Impello® biologicals
[0102] In some embodiments, the agricultural compositions of the present disclosure further comprise a biological from Impello®. In some embodiments, the biological is a microbial inoculant, biostimulating additive, or nutrient product from Impello®. In some embodiments, the product is Biofuel™, Continuum™, Dune™, Lumina™, Tribus®, or Tundra™. In some embodiments, the product is any of the products available from Impello, a list of which can be retrieved from the world wide web at: impellobio.com / collections / all.
[0103] In some embodiments, the biological comprises microbial soil inoculants. In some embodiments, the biological comprises species of B. subtilis, B. pumilus, B. amyloliquefaciens, B. licheniformis, Paenibacillus chitinolyticus and / or B. laterosporus. In some embodiments, the biological comprises species of B. subtilis, B. pumilus, B. amyloliquefaciens, and / or Paenibacillus chitinolyticus. In some embodiments, the biological comprises a commercially available soil inoculant. in some embodiments, the biological comprises Tribus® or Continuum™. In some embodiments, the biological comprises Bacillus subtillis (e.g., at about 4.0x109CFU / ml), Bacillus pumilus (e.g., at about 4.0x109CFU / ml), and Bacillus amyloliquefaciens (e.g., at about 2.0x109CFU / ml). In some embodiments, the biological comprises Paenibacillus chitinolyticus (e.g., at about 106CFU / mL), Bacillus subtillis (e.g., at about 106CFU / mL), Bacillus pumilus (e.g., at about 106CFU / mL), and Bacillus amyloliquefaciens (e.g., at about 106CFU / mL). Formulations
[0104] Agricultural and microbial compositions described herein and / or having characteristics as described herein can be in the form of a liquid, a foam, or a dry product. Compositions comprising microbial assemblages produced according to methods described herein and / or having characteristics as described herein may also be used to increase plant survivability and / or improve plant traits, such as plant biomass. In some embodiments, the composition is a liquid and has a total number of microorganisms of about 100,000 to about 100,000,000,000 colony forming units per milliliter (cfu / mL) (e.g. 100,000, 500,000, 1,000,000, 5,000,000, 10,000,000, 50,000,0000, 100,000,000, 500,000,000, 1,000,000,000, 5,000,000,000, 10,000,000,000, 50,000,000,000 and 100,000,000,000 cfu / mL). In some embodiments, the total number of microorganisms is about 100,000 to about 100,000,000,0000 cfu / mL, 100,000 to about 10,000,000,0000 cfu / mL, about 100,000 to about 1,000,0000,000 cfu / mL, aboutAttorney Docket No.: IMPE-005 / 02WO 342881-2039 100,000 to about 100,000,000 cfu / mL, about 100,000 to about 10,000,000 cfu / mL, about 100,000 to about 1,000,000 cfu / mL, 1,000,000 to about 100,000,000,0000 cfu / mL, 1,000,000 to about 10,000,000,0000 cfu / mL, about 1,000,000 to about 1,000,0000,000 cfu / mL, about 1,000,000 to about 100,000,000 cfu / mL, about 1,000,000 to about 10,000,000 cfu / mL, 10,000,000 to about 100,000,000,0000 cfu / mL, 10,000,000 to about 10,000,000,0000 cfu / mL, about 10,000,000 to about 1,000,0000,000 cfu / mL, about 10,000,000 to about 100,000,000 cfu / mL, 100,000,000 to about 100,000,000,0000 cfu / mL, 100,000,000 to about 10,000,000,0000 cfu / mL, about 100,000,000 to about 1,000,0000,000 cfu / mL, 1,000,000,000 to about 100,000,000,0000 cfu / mL, 1,000,000,000 to about 10,000,000,0000 cfu / mL, or 10,000,000,000 to about 100,000,000,0000 cfu / mL.
[0105] In some embodiments, the microorganisms are present at about 100,000 cfu / mL, about 200,000 cfu / mL, about 300,000 cfu / mL, about 400,000 cfu / mL, about 500,000 cfu / mL, about 600,000 cfu / mL, about 700,000 cfu / mL, about 800,000 cfu / mL, about 900,000 cfu / mL, about 1,000,000 cfu / mL, about 2,000,000 cfu / mL, about 3,000,000 cfu / mL, about 4,000,000 cfu / mL, about 5,000,000 cfu / mL, about 6,000,000 cfu / mL, about 7,000,000 cfu / mL, about 8,000,000 cfu / mL, about 9,000,000 cfu / mL, about 10,000,000 cfu / mL, about 20,000,000 cfu / mL, about 30,000,000 cfu / mL, about 40,000,000 cfu / mL, about 50,000,000 cfu / mL, about 60,000,000 cfu / mL, about 70,000,000 cfu / mL, about 80,000,000 cfu / mL, about 90,000,000 cfu / mL, about 100,000,000 cfu / mL, about 200,000,000 cfu / mL, about 300,000,000 cfu / mL, about 400,000,000 cfu / mL, about 500,000,000 cfu / mL, about 600,000,000 cfu / mL, about 700,000,000 cfu / mL, about 800,000,000 cfu / mL, about 900,000,000 cfu / mL, about 1,000,000,000 cfu / mL, about 2,000,000,000 cfu / mL, about 3,000,000,000 cfu / mL, about 4,000,000,000 cfu / mL, about 5,000,000,000 cfu / mL, about 6,000,000,000 cfu / mL, about 7,000,000,000 cfu / mL, about 8,000,000,000 cfu / mL, about 9,000,000,000 cfu / mL, about 10,000,000,000 cfu / mL, about 20,000,000,000 cfu / mL, about 30,000,000,000 cfu / mL, about 40,000,000,000 cfu / mL, about 50,000,000,000 cfu / mL, about 60,000,000,000 cfu / mL, about 70,000,000,000 cfu / mL, about 80,000,000,000 cfu / mL, about 90,000,000,000 cfu / mL, about 100,000,000,000 cfu / mL including all ranges, subranges and values therebetween.
[0106] In some embodiments, the microorganisms are present at about 90,000,000 to about 110,000,000 cfu / mL, about 95,000,00 to about 110,000,000 cfu / mL, about 100,000,000 to about 110,000,000 cfu / mL, about 90,000 to about 105,000,000 cfu / mL, about 95,000,000 to about 105,000,000 cfu / mL, about 100,000,000 cfu / mL to about 105,000,000 cfu / mL, about 90,000,000 to about 100,000,000 cfu / mL, or about 95,000,000 to about 100,000,000 cfu / mL.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0107] In some examples, one or more compositions may be coated onto a seed. In some examples, one or more compositions may be coated onto a seedling. In some examples, one or more compositions may be coated onto a surface of a seed. In some examples, one or more compositions may be coated as a layer above a surface of a seed. In some examples, a composition that is coated onto a seed may be in liquid form, in dry product form, in foam form, in a form of a slurry of powder and water, or in a flowable seed treatment. In some examples, one or more compositions may be applied to a seed and / or seedling by spraying, immersing, coating, encapsulating, and / or dusting the seed and / or seedling with the one or more compositions. In some examples, microbial assemblages can be coated onto a seed and / or a seedling of the plant. In some embodiments, one or more compositions may be used to inoculate a soil.
[0108] The methods and compositions may be applicable to a number of commercially important agricultural crops, for example, hemp, sorghum, canola, tomato, strawberry, pepper, barley, rice, maize, onion, and wheat. In some examples, compositions may be sprayed on the plant aerial parts, or applied to the roots by inserting into furrows in which the plant seeds are planted, watering to the soil, or dipping the roots in a suspension of the composition. In some examples, compositions may be dehydrated in a suitable manner that maintains cell viability and the ability to artificially inoculate and colonize host plants.
[0109] Compositions described herein can be formulated using an agriculturally acceptable carrier. The formulation useful for these embodiments may include at least one member selected from the group consisting of a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, a preservative, a stabilizer, a surfactant, an anti-complex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a fertilizer, a rodenticide, a desiccant, a bactericide, a nutrient, and any combination thereof.
[0110] In some examples, compositions may be shelf stable. For example, any of the compositions described herein can include an agriculturally acceptable carrier (e.g., one or more of a fertilizer such as a non-naturally occurring fertilizer, an adhesion agent such as a non- naturally occurring adhesion agent, and a pesticide such as a non-naturally occurring pesticide). A non-naturally occurring adhesion agent can be, for example, a polymer, copolymer, or synthetic wax. For example, any of the coated seeds, seedlings, or plants described herein can contain such an agriculturally acceptable carrier in the seed coating. In any of the compositions or methods described herein, an agriculturally acceptable carrier can be or can include a non-naturally occurring compound (e.g., a non-naturally occurring fertilizer, a non-naturally occurring adhesion agent such as a polymer, copolymer, or syntheticAttorney Docket No.: IMPE-005 / 02WO 342881-2039 wax, or a non-naturally occurring pesticide). Additional examples of agriculturally acceptable carriers are known in the art.
[0111] In some embodiments, the compositions of the present disclosure are solid. Where solid compositions are used, one or more carrier materials including, but not limited to: mineral earths such as silicas, talc, kaolin, limestone, chalk, clay, dolomite, diatomaceous earth; calcium sulfate; magnesium sulfate; magnesium oxide; products of vegetable origin such as cereal meals, tree bark meal, wood meal, and nutshell meal.
[0112] In some embodiments, the compositions of the present disclosure are liquid. In further embodiments, the liquid comprises a nutrient such as a carbon source, a nitrogen source, inorganic salts, as well as vitamins, amino acids, nucleic acids and the like. Examples of suitable carbon sources which can be used include, but are not limited to, starch, peptone, yeast extract, amino acids, sugars such as glucose, arabinose, mannose, glucosamine, maltose, corn steep powder, and the like. Salts of organic acids such as acetic acid, fumaric acid, adipic acid, propionic acid, citric acid, gluconic acid, malic acid, pyruvic acid, malonic acid and the like. Alcohols such as ethanol and glycerol and the like; oil or fat such as soybean oil, rice bran oil, olive oil, corn oil, sesame oil. Examples of suitable nitrogen sources which can be used include, but are not limited to, amino acids, yeast extract, tryptone, beef extract, peptone, potassium nitrate, ammonium nitrate, ammonium chloride, ammonium sulfate, ammonium phosphate, ammonia or combinations thereof. Inorganic and organic salts, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, magnesium chloride, monosodium glutamate, monopotassium phosphate, zinc sulfate, ferric sulfate, ferrous sulfate, ferric chloride, ferrous chloride, manganous sulfate, manganous chloride, zinc sulfate, zinc chloride, cupric sulfate, calcium chloride, sodium chloride, calcium carbonate, sodium carbonate can be used alone or in combination. Examples of additional nutrients include, but are not limited to, vitamins, nucleic acids, yeast extract, peptone, meat extract, malt extract, dried yeast and combinations thereof.
[0113] In some embodiments, the compositions of the present disclosure comprise thickening agents such as silica, clay, natural extracts of seeds or seaweed, synthetic derivatives of cellulose, guar gum, locust bean gum, alginates, and methylcelluloses. In some embodiments, the compositions comprise anti-settling agents such as modified starches, polyvinyl alcohol, xanthan gum, and the like.
[0114] In some embodiments, the compositions of the present disclosure comprise colorants including organic chromophores classified as nitroso; nitro; azo, including monoazo, bisazo and polyazo; acridine, anthraquinone, azine, diphenylmethane, indamine, indophenol, methine,Attorney Docket No.: IMPE-005 / 02WO 342881-2039 oxazine, phthalocyanine, thiazine, thiazole, triarylmethane, xanthene. In some embodiments, the compositions of the present disclosure comprise trace nutrients such as salts of iron, manganese, boron, copper, cobalt, molybdenum and zinc.
[0115] In some embodiments, compositions of the present disclosure comprise saccharides (e.g., monosaccharides, disaccharides, trisaccharides, polysaccharides, oligosaccharides, and the like), polymeric saccharides, lipids, polymeric lipids, lipopolysaccharides, proteins, polymeric proteins, lipoproteins, nucleic acids, nucleic acid polymers, silica, inorganic salts and combinations thereof. In a further embodiment, compositions comprise polymers of agar, agarose, gelrite, gellan gum and the like. In some embodiments, compositions comprise plastic capsules, emulsions (e.g., water and oil), membranes, and artificial membranes. In some embodiments, emulsions or linked polymer solutions may comprise microbial compositions of the present disclosure.
[0116] In some embodiments, compositions of the present disclosure occur in a solid form (e.g., dispersed lyophilized spores) or a liquid form (microbes interspersed in a storage medium).
[0117] In some embodiments, compositions of the present disclosure comprise one or more preservatives. The preservatives may be in liquid or gas formulations. The preservatives may be selected from one or more of monosaccharide, disaccharide, trisaccharide, polysaccharide, acetic acid, ascorbic acid, calcium ascorbate, erythorbic acid, iso-ascorbic acid, erythrobic acid, potassium nitrate, sodium ascorbate, sodium erythorbate, sodium iso-ascorbate, sodium nitrate, sodium nitrite, nitrogen, benzoic acid, calcium sorbate, ethyl lauroyl arginate, methyl-p- hydroxy benzoate, methyl paraben, potassium acetate, potassium benzoiate, potassium bisulphite, potassium diacetate, potassium lactate, potassium metabisulphite, potassium sorbate, propyl-p-hydroxy benzoate, propyl paraben, sodium acetate, sodium benzoate, sodium bisulphite, sodium nitrite, sodium diacetate, sodium lactate, sodium metabisulphite, sodium salt of methyl-p-hydroxy benzoic acid, sodium salt of propyl-p-hydroxy benzoic acid, sodium sulphate, sodium sulfite, sodium dithionite, sulphurous acid, calcium propionate, dimethyl dicarbonate, natamycin, potassium sorbate, potassium bisulfite, potassium metabisulfite, propionic acid, sodium diacetate, sodium propionate, sodium sorbate, sorbic acid, ascorbic acid, ascorbyl palmitate, ascorbyl stearate, butylated hydro-xyanisole, butylated hydroxytoluene (BHT), butylated hydroxyl anisole (BHA), citric acid, citric acid esters of mono- and / or diglycerides, L-cysteine, L-cysteine hydrochloride, gum guaiacum, gum guaiac, lecithin, lecithin citrate, monoglyceride citrate, monoisopropyl citrate, propyl gallate, sodium metabisulphite, tartaric acid, tertiary butyl hydroquinone, stannous chloride, thiodipropionicAttorney Docket No.: IMPE-005 / 02WO 342881-2039 acid, dilauryl thiodipropionate, distearyl thiodipropionate, ethoxyquin, sulfur dioxide, formic acid, or tocopherol(s).
[0118] In some embodiments, the compositions of the present disclosure are encapsulated. In some embodiments, encapsulating compositions encapsulate the microbial compositions in an adhesive polymer that can be natural or synthetic without toxic effect. In some embodiments, the encapsulating composition may be a matrix selected from sugar matrix, gelatin matrix, polymer matrix, silica matrix, starch matrix, foam matrix, etc. In some embodiments, the encapsulating composition may be selected from polyvinyl acetates; polyvinyl acetate copolymers; ethylene vinyl acetate (EVA) copolymers; polyvinyl alcohols; polyvinyl alcohol copolymers; celluloses, including ethylcelluloses, methylcelluloses, hydroxymethylcelluloses, hydroxypropylcelluloses and carboxymethylcellulose; polyvinylpyrolidones; polysaccharides, including starch, modified starch, dextrins, maltodextrins, alginate and chitosans; monosaccharides; fats; fatty acids, including oils; proteins, including gelatin and zeins; gum arabics; shellacs; vinylidene chloride and vinylidene chloride copolymers; calcium lignosulfonates; acrylic copolymers; polyvinylacrylates; polyethylene oxide; acrylamide polymers and copolymers; polyhydroxyethyl acrylate, methylacrylamide monomers; and polychloroprene.
[0119] In some embodiments, the agricultural compositions of the present disclosure are formulated as a crop generalist soil inoculant. In some aspects, the agricultural compositions of the present disclosure are formulated as a seed coating, a fertilizer additive, a spray, a drench, or combinations thereof. In some aspects, the agricultural compositions described herein are encapsulated.
[0120] In some embodiments, the agricultural compositions described herein further comprise one or more of: a nutrient, a polymer, a salt, a sugar, a pesticide, a plant growth regulator, a beneficial agent, a biologically active agent, an herbicide, a bactericide, a fungicide, an insecticide, a virucide, a miticide, a nematocide, an acaricide, a rodenticide, an anti-algae agent, a biocontrol agent, another bacterial species, a fungal species, a compatibilizing agent, an antifoam agent, a cleaning agent, a sequestering agent, a drift reduction agent, a neutralizing agent, a buffer, a corrosion inhibitor, a dye, an odorant, a spreading agent, a penetration aid, a sticking agent, a binder, a thickening agent, an emulsifier, an antimicrobial agent, a fertilizer, an inert carrier, a polymer, and combinations thereof. In some aspects, the agricultural composition described herein further comprises one or more of Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0121] In some embodiments, the agricultural compositions and / or bacteria of the present disclosure are formulated as a kit for agricultural use. Plants for use with the disclosed compositions
[0122] The methods and bacteria described herein are suitable for any of a variety of plants. Non-limiting examples of suitable plants include Cannabis (hemp), tomato, maize, rice, wheat, barley, sorghum, millet, oats, rye triticale, buckwheat, sweet corn, sugar cane, Amaryllidaceous (onions), strawberries, pepper, lettuce, and asparagus.
[0123] In some aspects, the plant is a species from Solanaceae, Cannabaceae, Amaryllidaceous, Asteraceae, Solanum, Cucurbitaceae, Rutaceae, Rosaceae, Rubiaceae, Malvaceae, Musaceae, Poaceae, and Fabaceae. In some aspects, the plant is a species from Lactuca, Zea, Triticum, Oryza, Solanum, Glycine, Brassica, Phaseolus, Cucurbita, Capsicum, Vitis, Citrus, Malus, Prunus, Coffea, Theobroma, Musa, Sorgham, Pisuym, Hordeum, and Cicer.
[0124] In some examples, plants that may be obtained or improved using the methods and composition disclosed herein may include plants that are important or interesting for agriculture, horticulture, biomass for the production of biofuel molecules and other chemicals, and / or forestry. Some examples of these plants may include pineapple, banana, coconut, lily, grasspeas and grass; and dicotyledonous plants, such as, for example, peas, alfalfa, tomatillo, melon, chickpea, chicory, clover, kale, lentil, soybean, tobacco, potato, sweet potato, radish, cabbage, rape, apple trees, grape, cotton, sunflower, thale cress, canola, citrus (including orange, mandarin, kumquat, lemon, lime, grapefruit, tangerine, tangelo, citron, and pomelo), bean, lettuce, Panicum virgatum (switch), Sorghum bicolor (sorghum, sudan), Miscanthus giganteus (miscanthus), Saccharum sp. (energycane), Populus balsamifera (poplar), Zea mays (corn), Glycine max (soybean), Brassica napus (canola), Triticum aestivum (wheat), Gossypium hirsutum (cotton), Oryza sativa (rice), Helianthus annuus (sunflower), Medicago sativa (alfalfa), Beta vulgaris (sugarbeet), Pennisetum glaucum (pearl millet), Panicum spp. Sorghum spp., Miscanthus spp., Saccharum spp., Erianthus spp., Populus spp., Secale cereale (rye), Salix spp. (willow), Eucalyptus spp. (eucalyptus), Triticosecale spp. (triticum- 25 wheat X rye), Bamboo, Carthamus tinctorius (safflower), Jatropha curcas (Jatropha), Ricinus communis (castor), Elaeis guineensis (oil palm), Phoenix dactylifera (date palm), Archontophoenix cunninghamiana (king palm), Syagrus romanzoffiana (queen palm), Linum usitatissimum (flax), Brassica juncea, Manihot esculenta (cassaya), Lycopersicon esculentum (tomato), Lactuca saliva (lettuce), Musa paradisiaca (banana), Solanum tuberosum (potato),Attorney Docket No.: IMPE-005 / 02WO 342881-2039 Brassica oleracea (broccoli, cauliflower, brussel sprouts), Camellia sinensis (tea), Fragaria ananassa (strawberry), Theobroma cacao (cocoa), Coffea arabica (coffee), Vitis vinifera (grape), Ananas comosus (pineapple), Capsicum annum (hot & sweet pepper), Allium cepa (onion), Cucumis melo (melon), Cucumis sativus (cucumber), Cucurbita maxima (squash), Cucurbita moschata (squash), Spinacea oleracea (spinach), Citrullus lanatus (watermelon), Abelmoschus esculentus (okra), Solanum melongena (eggplant), Papaver somniferum (opium poppy), Papaver orientale, Taxus baccata, Taxus brevifolia, Artemisia annua, Cannabis saliva, Camptotheca acuminate, Catharanthus roseus, Vinca rosea, Cinchona officinalis, Coichicum autumnale, Veratrum californica, Digitalis lanata, Digitalis purpurea, Dioscorea 5 spp., Andrographis paniculata, Atropa belladonna, Datura stomonium, Berberis spp., Cephalotaxus spp., Ephedra sinica, Ephedra spp., Erythroxylum coca, Galanthus wornorii, Scopolia spp., Lycopodium serratum (Huperzia serrata), Lycopodium spp., Rauwolfia serpentina, Rauwolfia spp., Sanguinaria canadensis, Hyoscyamus spp., Calendula officinalis, Chrysanthemum parthenium, Coleus forskohlii, Tanacetum parthenium, Parthenium argentatum (guayule), Hevea spp. (rubber), Mentha spicata (mint), Mentha piperita (mint), Bixa orellana, Alstroemeria spp., Rosa spp. (rose), Dianthus caryophyllus (carnation), Petunia spp. (petunia), Poinsettia pulcherrima (poinsettia), Nicotiana tabacum (tobacco), Lupinus albus (lupin), Uniola paniculata (oats), Hordeum vulgare (barley), and Lolium spp. (rye).
[0125] Cannabaceae is a small family of flowering plants. As now circumscribed, the family includes about 170 species grouped in about 11 genera, including Cannabis (hemp, marijuana), Humulus (hops) and Celtis (hackberries). Members of the family are erect or climbing plants with petalless flowers and dry, one-seeded fruits. Hemp (Cannabis) and hop (Humulus) are two economically important species. Humulus is described as a twining perennial herbaceous plant which sends up new shoots in early spring and dies back to the cold-hardy rhizome in autumn. Example species of hop (Humulus) include Humulus americanus Nutt., Humulus cordifolius Miq., Humulus lupulus L., Humulus neomexicanus (A.Nelson & Cockerell) Rydb., Humulus pubescens (E.Small) Tembrock, Humulus scandens (Lour.) Merr. (syn. Humulus japonicus Siebold & Zucc.), and Humulus yunnanensis Hu.
[0126] Cannabis is a genus of flowering plants that includes at least three species, Cannabis sativa, Cannabis indica, and Cannabis ruderalis as determined by plant phenotypes and secondary metabolite profiles. In practice however, cannabis nomenclature is often used incorrectly or interchangeably. Cannabis literature can be found referring to all cannabis varieties as “sativas” or all cannabinoid producing plants as “indicas”. Indeed, the promiscuous crosses of indoor cannabis breeding programs have made it difficult to distinguish varieties,Attorney Docket No.: IMPE-005 / 02WO 342881-2039 with most cannabis being sold in the United States having features of both sativa and indica species. The present disclosure provides for Cannabis sp. (species) or Cannabis spp. (species pluralis), which comprises Cannabis sativa, Cannabis indica, and Cannabis ruderalis, as well as hybrids and variants thereof.
[0127] Solanaceae is a family of flowering plants that includes a number of agricultural crops, including tomatoes, potatoes, eggplant, and bell pepper. Modes of Application
[0128] The compositions of the present disclosure may be applied to any part of a host plant or the environs thereof. In some embodiments, the compositions are applied to the roots and / or the soil around the host plant. In some embodiments, the compositions are applied to the seeds of the host plant before, during or shortly after planting. In some embodiments, the compositions are applied to the seeds, seedlings, plants, or plant parts. Plant parts include seeds, seedlings, plant tissues, leaves, branches, stems, bulbs, tubers, roots, root hairs, rhizomes, cuttings, flowers, and fruits. In some embodiments, compositions of the present disclosure are applied to any area where a plant will grow including soil, a plant root zone and a furrow.
[0129] The compositions of the present disclosure can be applied at any time during the host plant life cycle. In some embodiments, the compositions of the present disclosure are applied shortly after planting, tillering, or sowing. In some embodiments, the compositions of the present disclosure are applied as a seed coating or soil treatment around the time of planting. In some embodiments, the compositions are applied 0-30 days after planting, sowing, or tillering. In some embodiments, the compositions are applied pre-blooming. In some embodiments, the compositions are applied post-blooming. In some embodiments, the compositions are applied at rooting, sprouting, flowering, fruit setting, ripening, or fattening. in some embodiments, the compositions are applied before or during a peak period of metabolic activity. In some embodiments, the compositions are applied during a period of host plant stress. In some embodiments, the compositions are applied during drought stress. In some embodiments, the compositions are applied before a predicted period of drought stress.
[0130] In some embodiments, the compositions are applied more than once. In some embodiments, the composition is administered 1-50 times per growing cycle, depending on the type of crop, the intensity, and the planting. In some embodiments, the compositions are applied periodically throughout the growing cycle. The compositions may be applied once a day, once a week, once every two weeks, or once a month. In some embodiments, the timing of composition application is based on field studies assessing the efficacy of application atAttorney Docket No.: IMPE-005 / 02WO 342881-2039 different time points. In some embodiments, the compositions are applied 1-10 times throughout the growing cycle of the host plant. In some embodiments, the compositions are applied 1-5 times throughout the growing cycle of the host plant.
[0131] The compositions described herein can be applied in a number of different ways. For small scale application of a liquid composition, backpack tanks, hand-held wands, spray bottles, or aerosol cans can be utilized. For larger scale application of liquid compositions, tractor drawn rigs with booms, tractor drawn mist blowers, airplanes or helicopters equipped for spraying, or fogging sprayers can all be utilized.
[0132] Under certain conditions, a liquid composition can be injected or otherwise applied into the soil below the surface. In this manner, the methods and compositions of the present disclosure can be used to inhibit, prevent or repel soil insects from contacting plants and / or feeding on plants. For example, corn root worms in the soil from contacting and / or feeding on plants. One common method of injecting such liquids into the soil is to provide a sharpened point on the lower end of a shank and a tube extending down the shank behind the point. As the point is moved forwardly through the soil, it opens a furrow and liquid conducted downwardly through the tube soaks into the soil in the furrow. This arrangement does not disperse the liquid laterally into the soil and the liquid is concentrated in a narrow band.
[0133] The compositions described herein can be delivered through an irrigation system. Irrigation is an artificial application of water to the soil. It is usually used to assist in growing crops in dry areas and during periods of inadequate rainfall. Additionally, irrigation also has a few other uses in crop production, which include protecting plants against frost, suppressing weed growing in rice fields and helping in preventing soil consolidation. Commonly used irrigation techniques include, but are not limited to, surface irrigation, localized irrigation, drip irrigation, sprinkler irrigation, center pivot irrigation, sub irrigation (seepage irrigation), manual irrigation using buckets or watering cans, automatic, non-electric irrigation using buckets and ropes, irrigation using stones to catch water from humid air, and irrigation with dry terraces.
[0134] The compositions described herein can be applied to any plant or any plant part grown in any type of media (e.g., soil, vermiculite, shredded cardboard, water).
[0135] The compositions described herein may be applied any time during the life cycle of a plant, during one or more stages of a plant’s life cycle, or at regular intervals of a plant’s life cycle, or continuously throughout the life of the plant. In some embodiments, the compositions described herein can be applied before, during and / or shortly after the plants are transplanted from one location to another, such as from a greenhouse or hotbed to the field. In anotherAttorney Docket No.: IMPE-005 / 02WO 342881-2039 aspect, the compositions described herein can be applied shortly after seedlings emerge from the soil or other growth media (e.g., vermiculite). Concentrations and Rates of Application
[0136] In some aspects, the agricultural compositions herein comprise between 1.0 x 103and 1.0 x 1012CFU / mL of a microbial population disclosed herein. In some aspects, the composition comprises a microbial population at a concentration of about 103, 104, 105, 106, 107, 108, 109, 1010, 1011, or 1012CFU / mL. In some aspects, the composition comprises a microbial population at a concentration of about 105CFU / mL. In some aspects, the composition comprises a microbial population at a concentration of about 106CFU / mL. In some aspects, the composition comprises a microbial population at a concentration of about 107CFU / mL. In some aspects, the composition comprises a microbial population at a concentration of about 109CFU / mL. In some aspects, the composition comprises a microbial population at a concentration of about 1010CFU / mL. In some aspects, the composition comprises a microbial population at a concentration of about 1011CFU / mL.
[0137] In some embodiments, the composition comprises about 0.5, 1, 2, 3, 4, 5, 6, 7, 8, or 9 x 106CFU / mL of a microbial population herein. In some embodiments, the composition comprises about 4 x 106CFU / mL of a microbial population herein.
[0138] In some embodiments, the composition is diluted to 0.1-10 mL per gallon of liquid medium (e.g., water or nutrient solution) prior to application. In some embodiments, the composition is diluted to about 0.5, 1, or 2 mL per gallon of liquid medium.
[0139] In some embodiments, the composition is applied at a rate of about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 liters per acre per month. In some embodiments, the composition is applied at a rate of about 1, 2, 3, 4, or 5 liters per acre per month.
[0140] In some embodiments, the composition is applied at a rate of about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 gallons per acre per month. In some embodiments, the composition is applied at a rate of about 0.5, 1, 2, 3, 4, or 5 gallons per acre per month. Marketable traits
[0141] In some embodiments, the disclosure teaches a method of improving a marketable trait of a commodity plant product, comprising applying the agricultural composition disclosed herein to a plant, plant part, or root zone.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0142] In some embodiments, the disclosure teaches a method for improving plant growth, increasing marketable yield, and / or improving a marketable trait of a commodity plant product, the method comprising: applying to a plant, plant part, or root zone at least one isolated bacterial species having a 16S nucleic acid sequence that shares at least 97% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8, and wherein the application of the at least one isolated bacterial species improves plant growth, increases marketable yield, and / or improves a marketable trait of a commodity plant product, compared to untreated plants.
[0143] In some aspects, the improved marketable trait is an increase in plant biomass, compared to untreated plants. In some aspects, the commodity plant product is a fruit or vegetable.
[0144] In some aspects, the improved marketable trait is increased uniformity, increased quality, or increased mass of a fruit or vegetable, compared to untreated plants. In some embodiments, other marketable traits are improved, such as lettuce head size and lettuce greenness.
[0145] In some aspects, the improved marketable trait is an increase in uniformity of a fruit or vegetable of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 110%, 120%, 130%, 140%, 150%, 160%, 170%, 180%, 190%, or 200% compared to untreated plants. In some aspects, the increase in uniformity of a fruit or vegetable is between 5-25%, 25-50%, 50-75%, 75-100%, 100-125%, 125-150%, 150-175%, or 175-200% compared to untreated plants.
[0146] In some aspects, the improved marketable trait is an increase in quality of a fruit or vegetable of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 110%, 120%, 130%, 140%, 150%, 160%, 170%, 180%, 190%, or 200% compared to untreated plants. In some aspects, the increase in uniformity of a fruit or vegetable is between 5-25%, 25-50%, 50-75%, 75-100%, 100-125%, 125-150%, 150-175%, or 175-200% compared to untreated plants.
[0147] In some aspects, the improved marketable trait is an increase in mass of a fruit or vegetable of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 110%, 120%, 130%, 140%, 150%, 160%, 170%, 180%, 190%, or 200% compared to untreated plants. In some aspects, the increase in mass of a fruit or vegetable is between 5-25%, 25-50%, 50-75%, 75-100%, 100-125%, 125-150%, 150- 175%, or 175-200% compared to untreated plants.Attorney Docket No.: IMPE-005 / 02WO 342881-2039
[0148] In some embodiments, the methods and compositions disclosed herein increases water use efficiency compared to untreated plants. In some aspects, the methods and compositions described herein increase plant survivability and / or improve a marketable trait of a commodity plant product for a plant under abiotic stress, such as drought stress. The composition or isolated bacterial species may be applied at the onset or prior to onset of abiotic stress, and may be applied two or more times. Application may be to the plant, plant part, root zone, or rhizosphere. For example, application may occur by: coating a plant seed with the at least one isolated bacterial species or agricultural composition, thereby resulting in a bacterial seed coating; coating a plant part with the at least one isolated bacterial species or agricultural composition; spraying the at least one isolated bacterial species or agricultural composition onto a plant part; spraying the at least one isolated bacterial species or agricultural composition into a furrow into which a plant or seed will be placed; drenching the at least one isolated bacterial species or agricultural composition onto a plant part or into an area into which a plant will be placed; spreading the at least one isolated bacterial species or agricultural composition onto a plant part or into an area into which a plant will be placed; broadcasting the at least one isolated bacterial species or agricultural composition onto a plant part or into an area into which a plant will be placed; or combining the at least one isolated bacterial species or agricultural composition with a fertilizer or other agricultural composition that will be applied to a plant, plant part, or root zone.
[0149] In some aspects, the at least one isolated bacterial species or agricultural composition is formulated as a solution, a wettable powder, a dusting powder, a soluble powder, an emulsion or suspension concentrate, a seed dressing or coating, a tablet, water-dispersible granules, water soluble granules, microencapsulated granules or suspensions, or as an irrigation component.
[0150] In some aspects, the bacterial seed coating comprises about 1×103to about 1×1012bacterial cells per seed. In some aspects, the at least one isolated bacterial species is applied at between 103and 1012colony forming units.
[0151] As will be understood by those skilled in the art, additional plant traits may be improved, such as: root biomass, root length, height, shoot length, leaf number, water use efficiency, overall biomass, yield, fruit size, fruit mass, grain size, photosynthesis rate, proteome expression, and metabolite production.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 BUDAPEST TREATY ON THE INTERNATIONAL RECOGNITION OF THE DEPOSIT OF MICROORGANISMS FOR THE PURPOSE OF PATENT PROCEDURES
[0152] The microbial deposits of the present disclosure were made under the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure (Budapest Treaty).
[0153] Applicants state that pursuant to 37 C.F.R. § 1.808(a)(2) “all restrictions imposed by the depositor on the availability to the public of the deposited material will be irrevocably removed upon the granting of the patent.” This statement is subject to paragraph (b) of this section (i.e.37 C.F.R. § 1.808(b)).
[0154] Biologically pure cultures of Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, Aeromicrobium ginsengisoli, and Sphingomonas melonis were deposited as consortium “IMB-24-MB” on April 8, 2025, with the Bigelow National Center for Marine Algae and Microbiota (NCMA), located at 60 Bigelow Drive, East Boothbay, Maine 04544, USA, and assigned NCMA Patent Deposit Designation number 202504005.
[0155] A biologically pure culture of Mesorhozobium huakii was deposited as “IMB24-B6” on April 8, 2025, with the Bigelow National Center for Marine Algae and Microbiota (NCMA), located at 60 Bigelow Drive, East Boothbay, Maine 04544, USA, and assigned NCMA Patent Deposit Designation number 202504004. EXAMPLES
[0156] The following examples are given for the purpose of illustrating various embodiments of the disclosure and are not meant to limit the present disclosure in any fashion. Changes therein and other uses which are encompassed within the spirit of the disclosure, as defined by the scope of the claims, will be recognized by those skilled in the art. Example 1: Preparations of microbial isolates and consortia of the present disclosure
[0157] Solutions containing either a microbial isolate or consortia were prepared using standard growth media. The isolates and consortia assessed included those described below in Table 10. Table 10: Bacterial isolates and exemplary consortia Composition name Components UTC Untreated control – water only +CK Sterile LBA fermentation brothAttorney Docket No.: IMPE-005 / 02WO 342881-2039 B1 Janibacter cremeus B2 Sphingomonas mali B3 Mesorhizobium ciceri B4 Pseudomonas thivervalensis B5 Sphingomonas oligoaromativorans B6 Mesorhozobium huakii B7 Aeromicrobium ginsengisoli B8 Sphingomonas melonis C1 Janibacter cremeus, Sphingomonas mali C2 Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis C3 Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, Mesorhizobium huakuii C4 Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, Aeromicrobium ginsengisoli C5 Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, Mesorhizobium huakuii, Aeromicrobium ginsengisoli C6 Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhizobium huakuii C7 Pseudomonas thivervalensis and Mesorhozobium huakii MB Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, Aeromicrobium ginsengisoli, and Sphingomonas melonis MB2 Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis MB3 Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Mesorhozobium huakii, and Sphingomonas melonis (µ) ContinuµmTMPaenibacillus chitinolyticus, Bacillus subtilis, Bacillus pumilus, Bacillus amyloliquefaciens (µ) ContinuµmTM+ Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus B4 pumilus, Bacillus amyloliquefaciens, and Pseudomonas thivervalensis (µ) ContinuµmTM+ Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus B6 pumilus, Bacillus amyloliquefaciens and Mesorhozobium huakii
[0158] Approximately, 1x106and 1x108of bacterial cells per gram were added to a nutrient broth comprising glucose, yeast extract, monosodium glutamate, ammonium chloride, disodium phosphate (Na2HPO4), monopotassium phosphate (KH2PO4), magnesium sulfate,Attorney Docket No.: IMPE-005 / 02WO 342881-2039 manganese (II) sulfate (MnSO4), ferrous sulfate (FeSO4), and zinc sulfate (ZnSO4). The bacterial broth was adjusted to a pH of approximately 6.5. Example 2a: Increasing fruit mass in tomato
[0159] A composition comprising Janibacter cremeus was prepared as described above in Example 1. Plants of tomato cultivar ‘Ace 55’ were studied from November 1st2023 until February 14th, 2024. The plants were transplanted to their containers and allowed standard growing / irrigation conditions for up to two weeks before the drought stress was induced. Plants were then droughted for 4 days until treatments were applied with the irrigation water. Bacterial treatments were applied to plants with the first irrigation event following drought stress. The treatment was then applied every two weeks until the end of the study at 3 mL of bacterial culture per 1 gallon as a root drench, with irrigation solution and with bacterial culture mixed into the solution. Each treatment group consisted of 10 plants.
[0160] The following conditions were tested: 1. WW.CK: Adequate-watering conditions only (control), 2. WW.Q: Adequate-watering conditions plus B1 applied at experimental beginning, 3. SD.CK: Plants under drought only (control), 4. SD.Q.1x: B1 applies 1x at experiment beginning, under drought conditions, and 5. SD.Q.WK: B1 applied 1x per week at 3 mL per gallon, under drought conditions.
[0161] Results are in FIGs. 1-3. As shown, plants under adequate watering conditions performed better than those under drought, with and without the Janibacter cremeus treatment applied. In particular, plants under adequate water conditions that received Janibacter cremeus treatment had an average fruit mass of about 250g, while plants under adequate water conditions that that did not receive the treatment had an average fruit mass of about 200g, indicating that plants exposed to the bacterial treatment had about a 20% size increase (FIG. 1). Additionally, when plants under drought were grown with the Janibacter cremeus treatment (B1), increased fruit harvest parameters were observed compared to the plants under drought with no microbial species applied (FIGs. 1 and 2). Specifically, plants under drought that received the Janibacter cremeus treatment one time at the beginning of the study had an average fruit mass of about 75g, while plants that received no treatment had an average fruit mass of about 25g, indicating a 67% size increase (FIG. 1). Additionally, plants under drought that received the Janibacter cremeus treatment one time at the beginning of the study had an average fruit mass of about 150g, while plants that received no treatment had an average fruit mass of about 25g, indicating an 83% size increase (FIG. 1). Similar results were observed across treatment groups in an analysis of number of green fruit (FIG. 2). The aerial biomassAttorney Docket No.: IMPE-005 / 02WO 342881-2039 was reduced for plants under drought who received bacterial treatment at both the beginning (70g versus 90g, 22% decrease) and throughout the duration of the study (60g versus 90g, 33% decrease) in comparison to the untreated group under drought ((FIG. 3). While biomass was reduced under drought with bacterial treatment (FIG.3), it is hypothesized that the host-plant’s metabolism is devoting more resources to fruit production, as opposed to biomass synthesis. An exemplary image of a tomato plant from the different treatment groups in drought is shown in FIG.4. Example 2b: Bacterial isolates and consortia of the present disclosure improve marketable traits in beefsteak tomato variety ‘Defiant F1’
[0162] Compositions comprising various bacterial isolates and consortia were prepared as described above in Table 10, Example 1, and applied to beefsteak tomato variety ‘Defiant F1’.
[0163] Seeds were planted April 17, 2024, and 120 plants were randomized in eight different blocks of 15 plants each for the following treatment groups: UTC, C1, C2, C3, C4, C5, MB, and B4.
[0164] Drought stress was incurred by allowing plants to reach wilting point before being watered. Soil moisture was maintained between 45% - 55% water holding capacity except for after transplanting, and treatments (containers fully saturated). Time to wilt is variable based on the environment and size of plants, but 2 weeks after establishment, plants usually reached wilting point every 1-2 days. The first treatments were given 12 days before drought stress (water restriction), which began on May 15, 2024.
[0165] Watering was controlled by drip irrigation. For each minute of irrigation plants received 65 mL of water. For the first 4 weeks, plants received no more than 1 minute (65 mL) per 24 hours. Every 2 weeks thereafter, 1 minute of irrigation time was added. The final irrigation time was 5 minutes per day, meaning no more than 325 mL of water per 24 hours. This was sufficient water to allow plants to reach wilting point, but not reach permanent wilting point.
[0166] Plants received respective treatments as a soil drench at a concentration of 1 mL / gallon. 500 mL of solution was applied per plant. A total of 5 treatments were applied, every 2 weeks from May 3, 2024 through June 28, 2024. Data was collected during harvest on July 2, 2024, summarized below in Table 11.
[0167] Largest fruit size was measured as the diameter or latitudinal width of a single cut fruit. Fruit uniformity was measured on a scale of 1-5, with 1 = drastic differences in fruit ripeness and greater than 20 mm difference in fruit size, and over 80% of branches missing fruit; 2 =Attorney Docket No.: IMPE-005 / 02WO 342881-2039 differences in fruit ripeness and greater than 10 mm size. several branches missing fruit; 3 = some differences in fruit ripeness and size (between 2-5 mm), one or two branches missing fruits; 4 = nearly every fruit is the same ripeness and size, no branches missing fruit; 5 = all fruits are red and ripe and of large size, no branches missing fruit.
[0168] Fruit quality was measured on a scale of 1-5, with 1 = very low yield (between 100g - 200g), fruit diameter < 25 mm, numerous fruit that are split and rotting, poor uniformity; 2 = below average yield (between 200g - 300g), fruit diameter < 50 mm, some splitting; 3 = average yield of between 300g - 400g, fruit larger than 50mm, several red / ripe fruits, minor splitting or cracking, fruit is marketable, but not outstanding; 4 = above average yield of between 400g - 500g, nearly all fruit is red and ripe, no splitting or cracking, great uniformity, preferred for marketability; and 5 = outstanding yield over 500g, all fruit red and ripe, no splitting or cracking.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 y3 4r5.2. 32. 13. 02. 75. 00. 33dl)g 8 0 1 6 8 3 9.3(1 2 1 0 1 2 1 2ai s 1 1 1 1 1 1 1 1resaA m 79 5 9 4l)9.ag 4 9.4 1.4 3.7. 96. 17. 91.i(rh s376776 4 2 8 97675 8 3 6es7 7 7 7esaArfm 28. 0 1 9 2 8 324.3 1.3 1.3 3.3 2.3 2. 630.3tyitiul)ra5Fu-q1(yti66. 01. 59. 70. 50. 00. 7019m2 3 2 3 3 3.3.2artoaf )din5-4U12(02t,o 70. 72. 00. 60. 51. 33. 00. 70.2rd0 0 0 0 0 0 0 0yflonuJ# et / ti’ g1 vilpuFASrFtnaiffg 0435359174023333eon.0.0.0.0.0.0.0.0D‘#gtiikafvu eoArfrbyra fmoti 3u5. 000. 315. 60. 37. 02. 72. 39.m #r0 1 0 1 0 0ugfSvd:Ae1r1e fl o7b#a gt8. 012. 700. 6 7 0 7 730.0 8.0 2.2 8.0.viu 2 2 2 2 2 28191TArFt7s6. 72. 76. 00. 76. 79. 37. 31.e250 4 3 4 3 5 6gti) 6 5 5 5 5 5 5ra um Lrfm(tiu) 1rg 869383 6 4 5 7f(s.7.67.03 5.54 0.22 1.7.6.7174 6gvsa 4 5 4 4 4 45414A M l2.attoisus 7 4.5.4.9.1.2.0a19 7 2 0 7 1501760 9 8 6 227867 0 0 86Trfm6 7 7 6tnemtaerCT 4 1 2B T U B C C3C4C5C MAttorney Docket No.: IMPE-005 / 02WO 342881-2039
[0169] Treatment with B4 produced the greatest total and average fruit mass, the largest fruit, and had the highest average scores for fruit uniformity and fruit quality. However, all treatments improved fruit uniformity (increasing fruit uniformity scores between about 9% and about 16.5%) and fruit quality (increasing fruit quality scores between about 8.5% and about 20.5%) compared to untreated control plants. Example 2c: Bacterial isolates and consortia of the present disclosure improve marketable traits in cherry tomato variety ‘Washington Cherry’
[0170] Compositions comprising various bacterial isolates and consortia were prepared as described above in Table 10, Example 1, and applied to cherry tomato variety ‘Washington Cherry’.
[0171] Seeds started indoors on May 1, 2024 and transplanted into the field on May 29, 2024. 19 replicates of well-watered control pants were planted in a separate row.27 replicates of all other groups were planted in randomized groups of 3, in three, 100’ long, single rows. Two drip tape lines per row, 0.26 GPH / foot. Treatments comprised the following compositions: B4, B6, C6, MB, and MB2. In addition to the untreated control group (UTC), there was also a well-watered group.
[0172] Plants were treated by adding 500 mL of treatment solution to each plant. Solution concentrations were all 1 mL / gallon for all respective treatments. Plants first were treated 16 days after planting on June 20, 2024. Two more treatments were applied on July 12, 2024 and July 25, 2024.
[0173] Drought was induced by giving the drought rows half of the irrigation time of the “Well Watered” rows. For instance, plants in the well-watered group received 60 minutes of irrigation 3 days a week or 52 gallons per row per week. Plants in the drought group received 30 minutes of irrigation 3 days a week or 26 gallons per row per week.
[0174] Plants were harvested and final data collected August 7, 2024. Largest fruit diameter, fruit uniformity, and fruit quality were measured as described above in Example 2b.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 yti45. 529. 221. 637. 70. 41. 47.m2 3 3 2rofinU.g)v5- A1(69 5 1)6.2 9.2 1.3 9. 521. 330. 77.5- 3 2tiu 1r(Fy.ti glvauA Q 02. 22. 80. 83314898at93143.0.1.3.1ati 4 4 4 4 4d4u2r )0Fm2 t, sm7 e( gts rm aaiugL DuA6’1. 292. 21. 80. 71. 08. 98.yrdr e 2538473150304ehR.Conn.otgtiugvrnAFihsase2 9 1 2 0 4 3i9Wr.8 8.3.4.7.6 5‘r 7083. .93 7 3 1fe 1 1 161919161oBy.roanmgvmAuS:)2g1( 82s. 114. 803. 244. 765. 884. 36.es 2 17 9la 7 75475847894bamT hserfgvA 46162688878 0ss.a1.1.4.3.1 5.2 8.m2801821072642107 729 7y1 1rrebgv)Ag(tnemtae C2 Cr T 4 6 6B B W T U B B C M M WAttorney Docket No.: IMPE-005 / 02WO 342881-2039
[0175] All treatments increased the number of red fruits. Treatments B6 and MB produced more red fruit compared to other groups. There was a 65.02% increase in red fruit for those plants treated with B6 compared to the untreated control group, and a 17.68% increase over the well-watered group. There was a 75.48% increase in red fruit for those plants treated with MB compared to the untreated control group, and a 25.14% increase compared to the well- watered group. Similar increases in fruit quality and uniformity were also observed for all treatment groups compared to the untreated control group, as well as the well-watered group.
[0176] Additionally, plants treated with MB2 had significantly larger fruit, as well as increased fruit quality and uniformity scores, creating a more marketable crop. Example 2d: Bacterial isolates and consortia of the present disclosure improve marketable traits in beefsteak tomato variety ‘Defiant F1’
[0177] Compositions comprising various bacterial isolates and consortia were prepared as described above in Table 10, Example 1, and applied to beefsteak tomato variety ‘Defiant F1’.
[0178] Seeds were planted October 18, 2024, and a total of 198 plants were randomized in 11 different blocks of 18 plants each for the following treatments: B4, B6, C6, C7, MB, MB2, MB3, ContinuµmTM, ContinuµmTM+ B4, ContinuµmTM+ B6, and an untreated control (UTC).
[0179] Plants received respective treatments as a soil drench at a concentration of 1 mL / gallon. 500 mL of solution was applied per plant. A total of 5 treatments were applied, every 2 weeks from November 8, 2024 through December 20, 2024.
[0180] Drought stress was induced by allowing plants to reach wilting point before being watered. Soil moisture was maintained between 45% - 55% water holding capacity except for after transplanting, and during treatments (containers fully saturated). Time to wilt is variable based on the environment and size of plants, but 2 weeks after establishment, plants usually reached wilting point every 1-2 days.
[0181] Watering was controlled by drip irrigation. For each minute of irrigation plants received 65 mL of water. For the first 4 weeks, plants received no more than 1 minute (65 mL) per 24 hours. Every 2 weeks thereafter, 1 minute of irrigation time was added. The final irrigation time was 5 minutes per day, meaning no more than 325 mL of water per 24 hours. This was sufficient water to allow plants to reach wilting point, but not reach permanent wilting point.
[0182] Plants were harvested and data was collected on January 20, 2025. Largest fruit diameter, fruit uniformity, and fruit quality were measured as described above in Example 2b. Marketable fruit mass comprised fruit wider than 21 mm. Results are shown in Table 13 and FIG.5.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 ss 5.7 5 6 2 3 8 5 86.7.6.5.5.3.7.8.6 74.3.la 3 3 3 3 3 3 3 3 3 353gavim ry )Aeardg()2.5.1.6.3.2.4.9.2.6.4.lg(1079185978565009666788ga s 3 2 2 2 2 2 3 2 2 2 2vir hsesaAearfm 8.9.2.0.6.1 2 5 1 1 31. . . . . .yt1 2 2 2 2 1 1 2 2 1gilva )u5- Aq1(yti 3.7 8 8 2 9 2 3 9 2 11. . . . . . . . . .m1 1 1 2 1 1 1 1 2 1rofi)n5- U1(0.0.0.0.0.0 0 0 0 0 00 0 0 0 0.0.0. . . .til / tt0 0 0 0iop urS rd# Fnet4.1 72.5.09.29.9 7 2 2 6 16.0. . . . .s 3 3 3 3 4 44 5 9 9 4e2 2 3 3 2g t )riaum LrFm(gn 1.00.10.00.00.00.10.00.1 0 10. . .#ik 0 0 0.gvaeArb#2.3 5 3 4 2 0 3 4 1 20.0. . . . . . . . .g0 0 0 0 0 0 0 0 0v dAerel7.9.7.6.0.4.2.4.7.7.5ba0 4 0 0 1 0 0 0 0 0.0#tegk tvraiuA mrfgti2.9.2.1.4.9.3.7.5 1 6v u 2 1 2 2 3 1 1 1. . .r3 2 1A# felb5.6a 4.72.48.92.0 5 0 5 7 06.2. . . . .t)eg 5 4 5 5 8 33213643543gk(vrsasaA m m )6 6 1 6 6g5.88.1 .4.6.15.6.7..4.58.gt(s9 91 2 7 5855863 0 7i6v us1 1 1 1 1raAFM 151.0.0.33.08.30.59.30.46.3.6.l s 6 7 8 3 0 322009ati s 27350 2 0 51t u a ) 2 2 3 10 2 4 9 2otrfmg(7161 1 1 2 1 1tnemtaerC2 3T 4 6 6 7B B B4+6T U B B C C M M Mµ µ+µAttorney Docket No.: IMPE-005 / 02WO 342881-2039
[0183] Treatment with C7 (Pseudomonas thivervalensis and Mesorhizobium huakuii) showed statistically significant increase in total yield and marketable yield, as well as other quality metrics compared with the untreated control group and other treatment groups. B6 also increased the number of red fruit, suggesting that it helps plants focus on producing marketable fruit even under drought stress. Example 3a: Bacterial isolates of the present disclosure increase biomass of lettuce variety ‘Ice Queen’
[0184] A composition comprising Janibacter cremeus was prepared as described above in Example 1. Plants of lettuce cultivar ‘Ice Queen’ were studied from November 1st2023 until February 14th, 2024. Plant growth and treatment was performed as described in Example 2. Each treatment group consisted of 10 plants.
[0185] The following conditions were tested. 1. WWCK: Adequate-watering conditions only (control) 2. WWP (1 mL): Adequate-watering conditions plus 1 mL per gallon B1 at drought-onset 3. WWP (2 mL): Adequate-watering conditions plus 2 mL per gallon B1 at drought-onset 4. SDCK: Plants under drought only (control) 5. SDP: Plants under drought + 1 mL per gallon B1 at drought-onset
[0186] Results are in FIGs. 6-8. As shown, plants under adequate-watering conditions performed better than those under drought, with and without the Janibacter cremeus treatment applied. In particular, plants under adequate water conditions that received Janibacter cremeus treatment (2 mL per gallon B1 at drought-onset) had an average fresh weight of about 62g, while plants under adequate water conditions that that did not receive the treatment had an average fruit mass of about 50g, indicating that plants exposed to the bacterial treatment had about a 19% size increase.
[0187] Additionally, when plants under drought were grown with the B1, there was increased plant fresh weight (15g control versus 22 g treatment, 32% increase) (FIG.6), similar height of lettuce (8.5 cm control versus 8.3 cm treatment) (FIG.7, and increased plant width (11 cm control versus 13 cm treatment, 15% increase) (FIG.8) compared to the plants under drought with no bacterium applied.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 Example 3b: Bacterial isolates and consortia of the present disclosure improve marketable traits in lettuce variety ‘Ice Queen’
[0188] Compositions comprising various bacterial isolates were prepared as described above in Table 10, Example 1, and applied to lettuce variety ‘Ice Queen’ along with a positive control (+CK) comprising sterile LBA fermentation broth.
[0189] Seeds were planted in 4-inch containers and filled with 150 g of Miller Soil’s CDM Pro. The first treatment was applied after two sets of true leaves emerged on April 9, 2024. Treatments were prepared at 1 mL / gallon. 200 mL of respective treatment solution was applied per plant. Treatments were applied two more times on April 26, 2024, and May 10, 2024.
[0190] Drought stress was incurred by allowing plants to reach wilting point before being watered. Plants were irrigated to a maximum 60% water holding capacity except for after treatments (containers fully saturated). Plants were placed in randomized groups on 10-inch x 20-inch propagation trays. Once plants began to wilt, trays were weighed. After calculations the plants were adjusted to 60% water holding capacity by hand.
[0191] Plants were harvested and final data collected on June 7, 2024. Fresh mass was measured by weight in grams. Signs of bolting was measured on a score of 0-3, where 0 = no signs of bolting, plants producing vegetative leaves with smooth edges; 1 = early signs of bolting, "head" formation has ended and beginning to open, new leaves showing rougher edges; 2 = presence of seed head emerging 1 mm – 5 mm long; and 3 = seed head has fully emerged from the "head", over 5 mm long.
[0192] Lettuce head formation was measured on a scale of 0-5, with 0 = no head formation (0 mm width), indication of bolting; 1 = weak head formation, 5 – 30 mm in width, indication of early bolting; 2 = some head formation, over 30-50 mm in width, able to peel apart leaves; 3 = average head formation, 50- 70 mm in width, dense head, unable to peel apart leaves; 4 = large, dense head, 70 - 90 mm in width; and 5 = unusually large and dense head, over 90 mm in width.
[0193] Lettuce greenness was measured on a scale of 1 to 5, with 1 = over 50% of foliage shows yellowing; 2 = 15% or more of foliage shows yellowing; 3 = average looking, less than 5% of foliage shows yellowing; 4 = 0% yellow foliage, leaves noticeably a darker green than other plants; and 5 = 0% yellow foliage, large and very dark green leaves.
[0194] Lettuce quality was measured on a scale of 1 to 5, with 1 = low yield (less than 50 g), no head formation, poor vigor, and yellow leaves; 2 = below average yield (less than 70 g), poor head formation, some yellowing; 3 = average yield (80-90 g), average head formation, marketable plant; 4 = above average yield (greater than 90 g), dense head formation, desirableAttorney Docket No.: IMPE-005 / 02WO 342881-2039 for marketability; and 5 = high yield (over 120 g), very large and dense head formation, extraordinary quality.
[0195] Dry mass was measured by weight in grams after 24 hours in a drying oven at 70 °C.
[0196] Results are shown below in Table 14 and FIG.9. All treatments increased fresh mass, head size, greenness, overall quality, and dry mass. Isolate B6 - Mesorhozobium huakii produced the most-dense plants – having a 48% increase in fresh mass compared to untreated control plants, and had the highest scores for head size, greenness, quality, and dry mass, compared to the other treatments, while also having a low bolting score. Table 14: Summary of ‘Ice Queen’ June 7, 2024 data Fresh Mass Head Greenness Quality Dry Mass cumulative Bolting (g) (0-5) (1-5) (1-5) (g) score (0-3) UTC 72.41 1.03 2.53 2.45 7.56 85.98 0.19 B1 90.04 2.34 3.06 3.11 9.18 107.73 0.23 B2 89.65 1.34 3.11 2.55 9.86 106.51 0.88 B3 88.17 2.06 3.06 3.02 9.31 105.62 0.66 B4 84.73 2.59 3.31 3.30 9.29 103.21 0.34 B5 87.80 2.63 3.56 3.84 9.26 107.09 0.28 B6 107.16 3.39 3.69 3.89 9.66 127.78 0.28 B7 86.54 2.13 3.25 3.00 8.78 103.70 0.61 B8 88.31 2.19 3.20 3.14 9.11 105.95 0.43 MB 88.31 2.19 3.20 3.14 9.11 105.95 0.43 C1 88.31 2.19 3.20 3.14 9.11 105.95 0.43 Example 3c: Bacterial isolates and consortia of the present disclosure improve marketable traits in lettuce variety ‘Crispino’ A. September 5, 2024 - November 6th‘Crispino’ study
[0197] ‘Crispino’ plants were seeded in 128 cell plug liners on September 5, 2024. Plugs were transplanted into 4-inch containers on September 23, 2024. Each treatment group (11 in total) comprised 20 plants (220 plants total). Compositions B4, B6, C6, C7, MB, MB2, MB3, ContinuµmTM, ContinuµmTM+ B4, and ContinuµmTM+ B6, were prepared as described in Table 10, Example 1. Plants were treated on September 27th, October 11thand October 25th(14-day intervals). Treatments were prepared at 1 mL / gallon. 200 mL of respective treatment solution was applied per plant.
[0198] Drought stress was incurred by allowing plants to reach wilting point before being watered. Plants were irrigated to a maximum 60% water holding capacity, except for after treatments (containers fully saturated). Time to wilt is variable based on the environment andAttorney Docket No.: IMPE-005 / 02WO 342881-2039 size of plants. Plants were placed in randomized groups on 10-inch x 20-inch propagation trays. Once plants began to wilt, trays were weighed. After calculations the plants were adjusted to 60% water holding capacity by hand. Plants were harvested and final data collected on November 6, 2024, summarized below in Table 15 and shown in FIG.10.
[0199] Fresh mass was measured by weight in grams. Signs of bolting, lettuce head formation, greenness, and quality were measured as described above in Example 3b. Dry mass was measured by weight in grams after 24 hours in a drying oven at 70 °C.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 E316 6 5 4 4 40101010101015015 7 301 1 1U.0.0.0.0.0.0.0.0 0.0 0.0 0.W0- )WL / Dg(E9U940. 280 1. 26 3 6 3 8 6 2 60 1. 201. 101. 001. 21. 01. 01. 31. 90.W0 0 0 0 0 0_ )LW / FG(87. 39735286544981991769) 2.1. . . . . . . . .0221229 5 0 6 4 3 3 721 3 3 2 0 0 9 9O2 2 2 2 2 22 deL1 1HsUm(O) 3530788298034 8 0 6 72L.7.8.4.3.3.7 2.9 0.6.7.0.Hm9 7 1 6 1 60 1 6 3l(2 9 9 8 90 4 3 0 1a d 9 9 9 95 7 3 1 3 8t e9 9 9 9 9 9 8oTsU 5g 1. 001. 301. 800. 01. 31. 10. 14. 84. 11. 91.ni0 0 0 0 0 0 0 1tl)o3- B0(e 8vi2t. 9a8 7. 881. 499. 180. 183. 242. 7 5 1 119.2 9.1.4.l2 3 3 3 3 3 4 3138392umeuroCcs)g8(64436020412327910823s.s2.3.3.3.3.3.3.2.3.3 6.2yr aD M 6027753766150 0 1 1 0yt .il 2.2.2.2.2.2 6.3 0.3 9.2 4.3 9.2a)u5- Q1(ss8 5 7 3 4 1 1 7 6 4e3n.7.6.8.0.5.2.8.1.2. 61.n2 2 2 2 3 2 3 2 3 3 3ee)r5- G1(80. 113. 420. 824. 522. 427. 12. 51. 82. 46. 37.d 1 3 2 1 2 1a)e5- H0(9s075821766437989064500s.a )0. . . . . . . . . .272827262427212125291Mg(tnemtaerC2 3T 4 6 6 7B B B4+6T U B B C C M M Mµ µ+µAttorney Docket No.: IMPE-005 / 02WO 342881-2039
[0200] As shown in Table 15 and FIG.10, application of compositions MB2, B6, B4, C7 and Continuum + B4 produced the highest increase in yield compared to all groups. MB2 showed the most significant improvement in head development (approximately 197% increase over UTC) and overall quality (approximately 75% increase compared to UTC) compared to all other treatments.
[0201] Additionally, from the water use data calculated we see treatment groups used a similar amount of water. However, MB2, B6, B4, and Continuum + B4 produced the most yield with the water given. This demonstrates a significant increase in plant water use efficiency or WUE. B. September 11, 2024 - January 27, 2025 ‘Crispino’ study
[0202] ‘Crispino’ plants were seeded in 128 cell plug liners on September 11, 2024. Plugs were transplanted into 4-inch containers on September 27, 2024. There were two irrigation groups per treatment, “well-watered” and “drought.” Each treatment group (14 in total) comprised (24) plants (336 plants total). Plants were treated on December 20, 2024 and January 10, 2025. Treatments of B4, B6, C6, ContinuµmTM, ContinuµmTM+B6, MB2, and UTC (as described above in Table 10, Example 1) were prepared at 1 mL / gallon. 200 mL of respective treatment solution was applied per plant. Plants were grown at a grower standard for 5 weeks, then drought restriction started on December 16, 2025. This model better simulates how farmers in the field experience restrictions in water.
[0203] Drought stress was induced by allowing plants to reach wilting point before being watered. Plants were irrigated to a maximum 60% water holding capacity, except for after treatments (containers fully saturated). Plants were placed in randomized groups on 10-inch x 20-inch propagation trays. Once plants began to wilt, trays were weighed. After calculations the plants were adjusted to 60% water holding capacity by hand. The “well-watered” group was readjusted to 100% water holding capacity before reaching wilting point. Plants were harvested and final data collected on January 27, 2025, summarized in Tables 15a (drought) and 15b (well-watered) below and FIGs.11A and 11B.
[0204] Fresh weight (FW) was measured by weight in grams. Signs of bolting, greenness, lettuce head formation, and quality were measured as described above in Example 3b. Lettuce head size was measured in millimeters. Above (Abv) and below (Blw) ground Dry weight (DW) was measured by weight in grams after 24 hours in a drying oven at 70 °C.Attorney Docket No.: IMPE-005 / 02WO 342881-2039)g8(2 28.35901 5821)g 6 96954 387.3 6.0 7.038.82. 84.(81. 57.28.193.86.983. 66.W1 1 11121W21313 2 2D1 1 1.DwlwlB B 13 8 2 8 0 2 ) 715812005 4 6)(8. 1 0 9 6 1 7 g3 6 6 59 3 0g93 3.4 2.4 0.(4 1.4 1.4 8. .4.4.4.4 8.4 9.4 8.4W3WD D.vvbbA A )210032000 1 53 8 3 2 9 3 23- 1 0 0 00 3 172. 300. 701. 901. 602. 7 900.0 2.0at0 .a( 0.0.0.0 0.0 0.0 0.0g)3dn-0ni(otitlioB 3459822158822 d9no ev585 5 9 8 5 94.4 6.3 6.8 0.5 8.1 0.1 6.1cdita 4. 267. 878. 31. 78. 39. 16.4 5 5 5 5 5 5el 7 74 4 9 0 3er1 1 1 1 1 1 1ru e8 8 9 8 8o ec t1 1 1 1 1 1 1s amwuro-lCcs)5- 81 88( 0. 032. 752. 122. 48 4le ) 2 2 4 6 4 6 713354w5-42640448584673yt3 3 3 3.3.3.3 51il2(. . . . . . .y3 3 3 3 3 3 3a0u2 ti ,laQ72u) 6 0 9 2 1 8yQ 2ra8 7 7 0 45 9- 8. 91( 2 9. 021. 131. 131. 331. 332.u )33naJ 5- 0. 71(3 3. 732. 036. 093 5. 123 4. 933.3ss ’e onssni ene pe sninr r eeGC‘ rf G 2969881388779 o6yr2645779 9 0 02.3 1.3 2.3 3.3 2.3 3.3 4.3 a 1m.3 4.3 4. 637. 638. 036. 031.4)m5- uSd)0(: abe5- H0(3219. 387 2. 385. 315. 372. 75 60. 1 16. elb2656576747173727a 4. 823. 98 8 3 26. 35. 03. 29. 96Tezi 181848580.98 2) s 7 8da)memm(Hm(745 8 8 2 5 5)g 2 8 5 5 4 7 82. 670. 294. 028. 101. 011. 189. ( 85. 40. 30. 83. 43. 43. 50.6 6 7 7 7 686W5 6 0 7 8 1 0F8 8 9 8 9 9 9)vg( bAtntenemtmaerC2 6taC2 6TTU4B6B6B C Mµ+ eµrTTU4B6B6B C Mµ+µAttorney Docket No.: IMPE-005 / 02WO 342881-2039
[0205] Under drought conditions, B4 and B6 demonstrated the greatest increase in lettuce head size, with B6 having the highest overall cumulative score for marketable traits. B6 also increased lettuce head size “well-watered” conditions, but the consortium MB2 had the highest overall cumulative score for marketable traits. Example 4a: Water Usage in Bell Pepper
[0206] In an additional study, the impact of various bacterial treatments was assessed on water usage of bell peppers plants of cultivar ‘Cali Wonder’ under drought stress. Treatments were prepared as described in Table 10, Example 1. However, for these experiments, the bacterial treatments were applied with irrigation water at 1 mL per gallon every two weeks. For irrigation, plants were transplanted into potting mix in 1 gallon pots, and grown under standard irrigation conditions for 25 days. Plants were then given no water and remained under drought until the bacterial-treatments were applied. Treatments began when plants were water-stressed (less than 72 hours before the first treatment). For the first treatment plants were watered with 500 mL of bacterial-inoculated irrigation solution, to re-adjust the water holding capacity of the substrate to approximately 55.0%.
[0207] The calculation for the water-holding capacity (WHC) of the substrate included an average measure of 72.0g for an empty 1-gallon pot and 630.0g for the amount of substrate (35% WHC) to fill a 1-gallon pot, therefore resulting in 702.0 g for the mass of a 1-gallon pot plus the substrate. Next a measure of the weight of the container plus the 100%-saturated substrate was estimated to be 1,572.0g and the weight of the container plus the substrate at 55% WHC was about 847.0g. Notably, during each irrigation-event the experimental plans were re- adjusted to 847.0 grams, and every two weeks the fresh-weight biomass of one experimental group from each target, was recorded and this mass was factored into the re-adjustment calculation to determine the amount of water needed to maintain the 55% WHC of substrate.
[0208] Based on calculations mentioned above, the plants were irrigated using 3.785.41 Liters per 60 minutes drip-emitters. These were switched on for a duration of 3 minutes per day, to provide approximately 195 mL of irrigation volume per day. Irrigation volumes was increased every two weeks, to account for increased host plant mass effecting 55% substrate-WHC calculations.
[0209] The bacterial treatment application method included applying 1 mL per gallon of irrigation solution for each application (equivalent of 300 µL per Liter). The first bacterial treatment(s) was applied at the first onset of drought, as a recovery to expose plant roots toAttorney Docket No.: IMPE-005 / 02WO 342881-2039 tested bacterial treatments. The isolates were then applied at the same rates every two weeks until the end of the experimental course.
[0210] The total volume of nutrient solution is calculated based on the amount of water (in mL) needed to re-adjust the substrate to 55% WHC. Five experimental plants are weighed, and the average mass of these is used to determine the re-adjustment volume. This per-replicate volume is then multiplied by the number of replicates within each treatment.
[0211] The planta data recording and harvested parameters were collected over the 10 weeks following the first treatment. Weekly measurements of substrate moisture content (via osmotic potential) will be recorded using soil sensor during each week for re-adjustment of WHC. The outcomes of the experiment to be recorded at the end include Plant above ground biomass, fruit yield, number of fruits, fruit quality, ripe fruit, total fruit number will be recorded.
[0212] In the bell pepper trial, the initial water usage (i.e. water depletion) was recorded for each bacterial treatment between irrigation events. Results are shown in FIGs.12A and 11B. These intervals were used to re-adjust the pot to saturation point, followed by the amount of water either used or retained by the plant-bacterial treatment. In FIG.12A, the "C1" treatment group being the highest on the graph indicated that the plants preserved the water holding capacity of the soil because of the bacterial treatment applied to those plants. For example, in C1 the first peak is about 0.27 m3 / m3VWC and then during the drought period the treated plant was able to use up 44% of the moisture content, with only 0.15 m3 / m3VWC remaining in the soil. Representative images comparing bell peppers in the control group versus treatment groups are shown in FIG.13. Example 4b: Bacterial isolates and consortia of the present disclosure improve marketable traits in bell pepper variety ‘Green Machine’ A. February 27, 2024 – April 24th‘Green Machine’ study
[0213] ‘Green Machine’ peppers were planted in 1-gallon containers with peat / perlite growing media. Plants were assigned treatment groups and arranged in randomized blocks. All groups contained 12 plants, except for the untreated control (UTC), positive control (CK), consortium C1, and MB, which each contained 11 plants. Isolates and consortia were prepared as described above in Table 10, Example 1.
[0214] Drought stress was incurred by allowing plants to reach wilting point before being watered. Soil moisture was maintained between 45% - 55% water holding capacity except for after transplanting, and treatments (containers fully saturated). Time to wilt is variable basedAttorney Docket No.: IMPE-005 / 02WO 342881-2039 on the environment and size of plants, but approximately two weeks after establishment, plants would reached wilting point every 1-2 days.
[0215] Watering was controlled by drip irrigation. For each minute of irrigation plants received 65 mL of water. For the first 4 weeks, plants received no more than 1 minute (65 mL) per 24 hours. Every 2 weeks thereafter, 1 minute of irrigation time was added. The final irrigation time was 3 minutes per day, meaning no more than 195 mL of water per 24 hours. This was sufficient water to allow plants to reach wilting point, but not the permanent wilting point.
[0216] Plants received respective treatments as a soil drench at a concentration of 1mL / gallon. 500 mL of solution was applied per plant. A total of 4 treatments were applied, every 2 weeks from February 27, 2024, through April 5, 2024. Treatment groups correspond to those described in Example 1. Data was collected during harvest on April 24, 2024. Fruit quality was measured on a scale of 1-5, with 1 being very deformed, rotting, or having severe necrosis, 3 being average looking, and 5 being of outstanding shape, having four lobes, uniform, and larger than average size.
[0217] Results are shown below in Table 17 and FIG. 14. B4 produced the highest average yield (306 grams per plant - a 4.29% increase over the untreated control group). B7, while producing the lowest average number of fruit per plant, produced the only fruit which would be of marketable quality, having an average quality score of 3.98 - an increase of 23.22% compared to the untreated control group. B7 also had the highest mass per fruit, averaging 64.7 grams, which is a 22.31% increase over the untreated control group. Table 17: Summary of ‘Green Machine’ April 24, 2024 drought data Avg no. of Total Avg fruit per Avg fruit Total yield Avg yieldberries mass / fruit plant quality UTC 3227.7 293.4 61 52.9 5.55 3.23 PosCk 3028.8 275.3 57 53.1 5.18 3.11 C1 3240.2 294.6 65 49.8 5.91 3.36 B1 3486.9 290.6 59 59.1 4.92 3.81 B2 3495.5 291.3 60 58.3 5 3.44 B3 3498.3 291.5 67 52.2 5.58 3.58 B4 3671.6 306.0 67 54.8 5.58 3.81 B5 3442.1 286.8 67 51.4 5.58 3.52 B6 3272.6 272.7 60 54.5 5 3.63 B7 2780.6 231.7 43 64.7 3.58 3.98 B8 3426.5 285.5 65 52.7 5.42 3.79 MB 3348.3 304.4 55 60.9 5 3.57Attorney Docket No.: IMPE-005 / 02WO 342881-2039 B. August 2, 2024 – October 7th‘Green Machine’ study
[0218] In another study, the following isolates and consortia were tested on drought-stressed ‘Green Machine’ pepper plants: B4, B6, MB, MB2, C6, Continuµm, and Continuµm + B4. Isolates and consortia were prepared as described above in Table 10, Example 1.
[0219] Plants were randomized in 8 different blocks of 24. Drought stress was induced by allowing plants to reach wilting point before being watered. Soil moisture was maintained between 45% - 55% water holding capacity except for after transplanting, and treatments (containers fully saturated).
[0220] Watering was controlled by drip irrigation. For each minute of irrigation plants received 65 mL of water. For the first 4 weeks, plants received no more than 1 minute (65 mL) per 24 hours. Every 2 weeks thereafter, 1 minute of irrigation time was added. The final irrigation time was 5 minutes per day, meaning no more than 325 mL of water per 24 hours. This was sufficient water to allow plants to reach wilting point, but not reach permanent wilting point.
[0221] Plants received respective treatments as a soil drench at a concentration of 1 mL / gallon. 500mL of solution was applied per plant. A total of 4 treatments were applied, every 2 weeks from August 2, 2024 through September 13, 2024. Data was collected during harvest, on October 7, 2024 and is summarized below in Table 18. Fruit quality was measured on a scale of 1-5, with 1 being very deformed, rotting, or having severe necrosis, 3 being average looking, and 5 being of outstanding shape, having four lobes, uniform, and larger than average size. Fruit uniformity was measured on a scale of 1-5, with 1 = misshapen lobes, multicolored fruit that is of different sizes (difference of greater than 50 mm in length); 2 = below average, some misshapen lobes, some blanching / discoloration (about 10%), some fruit of different sizes (difference of more than 30 mm in length)’ 3 = average uniformity, most fruit have uniform lobes, little to no discoloration, differences in fruit length less than 20 mm; 4 = above average, all fruits are square with equally developed lobes, no discoloration, differences in fruit length less than 10 mm; 5 = perfect uniformity, all fruits are square with equally developed lobes, no discoloration, differences in fruit length are less than 5 mm. Marketable fruits were those fruits that scored a 3 or higher for fruit quality, and weighed at least 60 g.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 4.l1 4.1 6.1 5.1 6.4.5.0.a) 1 1 1 111111111igr(eyArd)5.9 4.9 6.0 8.9 2.2.8.9.lga( 6 6 7 696969666irheseArf6.3 6.3 5.3 5.3 6.3 6.3 6.3 6.3yttiilur a)u5-FQ1(atad9 1 0tyti.2.3.3 9.2 1.3 1.3 2.3 2.3hgmuroorfi)d n5-4U1(202ti )4.,u 6 0.6 9.3 4.6 0.6 5.3.1.7rFm8 8 8 8 8587838rebtms(otghtcragnOLel’enit 3.3.4.5 3 5 4 3hek 1 1 1.1.1.1.1.1caraM M.tine ourerNFG‘fti 5.4.5.6.4.5.5.5.ur 1 1 1 1 1 1 1 1oyr Fa.om NmuS3.9.6.5.4 8 7 0:)8g 21 t(s42244248.6.3.3.223242525242eklrsabaM MT ss1a.1 6.6 7.0 4.2 5.6 0.1.0.m 5 4 5 4 44 1 9g2 2 2 2 2526242vA 4.7 8.8 3.7 8.7 6.6 8.2.6.2 1 1 1 1696 6lats0695068595006079osa 6 5T m tnemtaerC2 4TTU4B6B6B B C M Mµ+µAttorney Docket No.: IMPE-005 / 02WO 342881-2039
[0222] MB2 and Continuµm treatments increased the marketable yield, while also improving fruit uniformity. Additional data (not shown), suggests benefits to water use efficiency from applications of B6, C6, MB2, Continuµm and Continuµm + B4. C. November 1, 2024 – January 13, 2025 ‘Green Machine’ study
[0223] In another study, the following isolates and consortia were tested on drought-stressed ‘Green Machine’ pepper plants: B4, B6, C6, C7, MB, MB2, MB3, Continuµm, Continuµm + B4, and Continuµm + B6. Isolates and consortia were prepared as described above in Table 10, Example 1. Plants were planted on October 23, 2024 and were randomized into blocks of 18 plants per treatment.
[0224] Drought stress was induced by allowing plants to reach wilting point before being watered. Soil moisture was maintained between 45% - 55% water holding capacity except for after transplanting, and treatments (containers fully saturated).
[0225] Watering was controlled by drip irrigation. For each minute of irrigation plants received 65 mL of water. For the first 4 weeks, plants received no more than 1 minute (65 mL) per 24 hours. Every 2 weeks thereafter, 1 minute of irrigation time was added. The final irrigation time was 5 minutes per day, meaning no more than 325 mL of water per 24 hours. This was sufficient water to allow plants to reach wilting point, but not reach permanent wilting point. Plants received respective treatments as a soil drench at a concentration of 1 mL / gallon. 500 mL of solution was applied per plant. A total of 4 treatments were applied, every 2 weeks from November 1, 2024 through December 13, 2024. Data was collected during harvest on January 13, 2025 and is shown in FIG. 15 and summarized below in Table 19. Fruit quality was measured on a scale of 1-5, with 1 being very deformed, rotting, or having severe necrosis, 3 being average looking, and 5 being of outstanding shape, having four lobes, uniform, and larger than average size. Fruit uniformity and marketable fruits were determined as described above (B. August 2, 2024 – October 7th‘Green Machine’ study).Attorney Docket No.: IMPE-005 / 02WO 342881-2039 )g(6.4 3.6.4.6.6.0.1.3.0.9.131211121314131315 3yr 1 1dlaireA 9 h.s18.5 8.1 5.5 5.2 2.9 7.9 0.3 4.68.69.e09 9 8 9 9 9 9 969rf101laire)Ag(2.0.3.3.1.7 0 6 0 6 4tyt3 3 3 3 3.2.3.3.3.2.3aa tid ilt ur a)u5-hFQ1(guor y7.8.8.8.9.3.7.0.7.6.0.d ti 2 2 2 2 2 2 2 3 2 2 35m2r0o2fi)5,n -3U11(yrahut 3.6 3.2 2.6 6.8 3.1.6.2.6.4.6.natg 4 4 5 464921334430324sJ enl’lelt )eaii mumnrh S Fm(ca tMiu)5.0 2.5 2.0 0.2 1.8 4.0 7.4 9.4 2.5 5.5 4.9nrm7 6 7 7 6 6 6eFer tm(7 6 5 6sGght‘rfagnoLelyra 9.8 9 1 8t 0.0.0.1.0 6.0 8.0 1.1 9.0 3.0 6.1mm euS .k t: oraiuN Mr9F1el5b.a2 1.3 0.3 8.2 1.3 5.4 7.3 2.2 5.3 2.4 9.2T.toiuNrF0t.ks 99.26.5 3.14.7 0.2 8.83.84.7 8.17.5rsa )01 80121 8 6 831 9 451M Mg(tiu)8.3.0.0.6 4 4 5 7 7 1rgf(0 9 9 5.7.2.3.5.8.0.7s61314161414151615 4 9gs1 1 1vaam tnemtaerCTB2 3 4 6U4B B T B6B6C7C M M Mµ+µ+µAttorney Docket No.: IMPE-005 / 02WO 342881-2039
[0226] Continuµm in combination with B6 showed a significant increase in yield and marketable yield, having a 22.57% increase over the untreated control group for average fruit mass, and an increase of 42.84% for marketable fruit mass compared to the untreated control group. Continuµm + B6 also improved fruit uniformity and quality, and produced the least amount of small fruit, suggesting that B6 helps accelerate fruit growth under drought conditions. D. June 20, 2024 – September 4th‘Green Machine’ study
[0227] Field trials with bell pepper ‘Green Machine’ and the isolates and consortia of the present disclosure also showed that B6 improved fruit uniformity and yield under drought conditions.
[0228] ‘Green Machine’ seeds were started indoors on May 1, 2024. There were 32 plants in each treatment group, planted in randomized groups of 6, in three, 100’ long, double rows, and there were 20 plants in a “well-watered” control group planted in a separate row. One drip tape line per row, 0.26 GPH / foot. Plants were treated by adding 500 mL of treatment solution (B4, B6, C6, MB, or MB2, as prepared in Table 10, Example 1) to each plant. Solution concentrations were all 1 mL / gallon for all respective treatments. Plants first were treated 16 days after planting on June 20, 2024. Three more treatments were applied on July 12th, July 25thand August 16th.
[0229] Drought conditions were induced in the treatment groups by giving those rows half of the irrigation time of the “Well Watered” rows. For instance, well-watered plants received 60 minutes of irrigation 3 days a week or 26 gallons per row per week. Those under drought conditions received 30 minutes of irrigation 3 days a week or 13 gallons per row per week.
[0230] Plants harvested and final data collected September 4, 2024, summarized below in Table 20 and FIG. 16. Fruit quality was measured on a scale of 1-5, with 1 being very deformed, rotting, or having severe necrosis, 3 being average looking, and 5 being of outstanding shape, having four lobes, uniform, and larger than average size. Fruit uniformity and marketable fruits were determined as described above (B. August 2, 2024 – October 7th‘Green Machine’ study).Attorney Docket No.: IMPE-005 / 02WO 342881-2039 dle2i82 3 3 8y5196451 535 1 3l 5a24243 8 2313038262toT 5. 9 4 9 1 4 6W1 .27. . . . .74868484838D1gvA 6.4 8.5 4.2 7.1 0.9 4.8 6.W4F86405258413594agtvadAdle9i.fe 0 1.1 9.0 7.0 8.0 7.0 9.0tgh gaguvmorAadd4h 82t .5.0.9.1.3.00g 42n 14151414 4.4,e1 1 1l4rgevbA metyti 8.2 9.2 3.3 2.1.9.9.p3 3 2 2emSr’e govfinihAnuca 6.3 3.3 9.8.7.5.6.M3 3 3 3 3neyetrgilaGv‘Aufqo173 1 94 7yrs .asa9 .79 .4.5.1 .0.1mm 2 818 404 1610101 979mugvSA:02 5e e150 1. 5354 32 52l lb b .8.1 72 .1.9.8a at )g1 2 2 1 1Tek k(rsasaM M 18 512 00721310908el1 1bate.koraN M tnemtae2rwCT w4B6B6B B C M MTUAttorney Docket No.: IMPE-005 / 02WO 342881-2039
[0231] Treatment with B6 increased yield and significantly increased fruit uniformity and fruit length. Overall, B6 produced the highest amount of marketable yield, at 27.1 kg compared to drought untreated control at 18.25 kg and the well-watered group at 18.15 kg. This suggests that B6 produces higher quality fruit under drought conditions and well-watered conditions.
[0232] While embodiments of the present disclosure have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the disclosure. It should be understood that various alternatives to the embodiments of the disclosure described herein may be employed in practicing the disclosure. It is intended that the following claims define the scope of the disclosure and that methods and structures within the scope of these claims and their equivalents be covered thereby. INCORPORATION BY REFERENCE
[0233] All references, articles, publications, patents, patent publications, and patent applications cited herein are incorporated by reference in their entireties for all purposes. However, mention of any reference, article, publication, patent, patent publication, and patent application cited herein is not, and should not be taken as, an acknowledgment or any form of suggestion that they constitute valid prior art or form part of the common general knowledge in any country in the world. NUMBERED EMBODIMENTS 1. An agricultural composition comprising: a microbial population that comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the microbial population is present in the agricultural composition at between 1×103to 1×1012cells per gram. 2. The agricultural composition of embodiment 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 3. The agricultural composition of embodiment 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8. 4. The agricultural composition of embodiment 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid selected from the group consisting of SEQ ID NOs: 1-8. 5. The agricultural composition of any one of embodiments 1-4, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1. 6. The agricultural composition of any one of embodiments 1-5, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 1. 7. The agricultural composition of any one of embodiments 1-6, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 1. 8. The agricultural composition of any one of embodiments 1-7, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO: 1. 9. The agricultural composition of any one of embodiments 1-8, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2. 10. The agricultural composition of any one of embodiments 1-9, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 2. 11. The agricultural composition of any one of embodiments 1-10, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 2. 12. The agricultural composition of any one of embodiments 1-11, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO: 2. 13. The agricultural composition of any one of embodiments 1-12, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 14. The agricultural composition of any one of embodiments 1-13, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 3. 15. The agricultural composition of any one of embodiments 1-14, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 3. 16. The agricultural composition of any one of embodiments 1-15, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO: 3. 17. The agricultural composition of any one of embodiments 1-16, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4. 18. The agricultural composition of any one of embodiments 1-17, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 4. 19. The agricultural composition of any one of embodiments 1-18, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 4. 20. The agricultural composition of any one of embodiments 1-19, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO: 4. 21. The agricultural composition of any one of embodiments 1-20, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5. 22. The agricultural composition of any one of embodiments 1-21, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 5. 23. The agricultural composition of any one of embodiments 1-22, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 5. 24. The agricultural composition of any one of embodiments 1-23, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO: 5.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 25. The agricultural composition of any one of embodiments 1-24, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6. 26. The agricultural composition of any one of embodiments 1-25, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 6. 27. The agricultural composition of any one of embodiments 1-26, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 6. 28. The agricultural composition of any one of embodiments 1-27, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO: 6. 29. The agricultural composition of any one of embodiments 1-28, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 7. 30. The agricultural composition of any one of embodiments 1-29, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 7. 31. The agricultural composition of any one of embodiments 1-30, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 7. 32. The agricultural composition of any one of embodiments 1-31, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO: 7. 33. The agricultural composition of any one of embodiments 1-32, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 8. 34. The agricultural composition of any one of embodiments 1-33, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 8. 35. The agricultural composition of any one of embodiments 1-34, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 8.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 36. The agricultural composition of any one of embodiments 1-35, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO: 8. 37. The agricultural composition of any one of embodiments 1-36, wherein the at least one bacterium comprises Janibacter cremeus as deposited at NCMA 202504005. 38. The agricultural composition of any one of embodiments 1-36, wherein the at least one bacterium comprises Sphingomonas mali as deposited at NCMA 202504005. 39. The agricultural composition of any one of embodiments 1-36, wherein the at least one bacterium comprises Mesorhizobium ciceri as deposited at NCMA 202504005. 40. The agricultural composition of any one of embodiments 1-36, wherein the at least one bacterium comprises Pseudomonas thivervalenscomprises as deposited at NCMA 202504005. 41. The agricultural composition of any one of embodiments 1-36, wherein the at least one bacterium comprises Sphingomonas oligoaromativorans as deposited at NCMA 202504005. 42. The agricultural composition of any one of embodiments 1-36, wherein the at least one bacterium comprises Mesorhizobium huakuii as deposited at NCMA 202504004. 43. The agricultural composition of any one of embodiments 1-36, wherein the at least one bacterium comprises Aeromicrobium ginsengcomprisesoli as deposited at NCMA 202504005. 44. The agricultural composition of any one of embodiments 1-36, wherein the at least one bacterium comprises Sphingomonas meloncomprises as deposited at NCMA 202504005. 45. The agricultural composition of any one of embodiments 1-44, wherein the at least one bacterium comprises two isolated bacterial species. 46. The agricultural composition of any one of embodiments 1-44, wherein the at least one bacterium comprises between three and five isolated bacterial species. 47. The agricultural composition of any one of embodiments 1-44, wherein the at least one bacterium comprises between five and seven isolated bacterial species. 48. The agricultural composition of any one of embodiments 1-44, wherein the at least one bacterium comprises seven or eight isolated bacterial species. 49. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 50. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4. 51. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6. 52. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 7. 53. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2;Attorney Docket No.: IMPE-005 / 02WO 342881-2039 at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 7. 54. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6. 55. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6. 56. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6;Attorney Docket No.: IMPE-005 / 02WO 342881-2039 at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 7; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 8. 57. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 8. 58. The agricultural composition of any one of embodiments 1-48, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 8.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 59. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, and Sphingomonas mali, said isolated bacterial species deposited at NCMA 202504005. 60. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, and Pseudomonas thivervalensis, said isolated bacterial species deposited at NCMA 202504005. 61. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005. 62. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Aeromicrobium ginsengisoli, said isolated bacterial species deposited at NCMA 202504005. 63. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, Mesorhizobium huakuii, and Aeromicrobium ginsengisoli, said isolated bacterial species deposited at NCMA 202504005. 64. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005. 65. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Pseudomonas thivervalensis and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005. 66. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, Aeromicrobium ginsengisoli, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005. 67. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans,Attorney Docket No.: IMPE-005 / 02WO 342881-2039 Mesorhozobium huakii, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005. 68. The agricultural composition of any one of embodiments 1-48, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Mesorhozobium huakii, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005. 69. The agricultural composition of any one of embodiments 45-48, or 59-68, wherein each isolated bacterial species is present in the composition at approximately equal ratios. 70. An agricultural composition comprising: a microbial population that comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity to SEQ ID NO: 6; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the microbial population is present in the agricultural composition at between 1×103to 1×1012cells per gram. 71. The agricultural composition of embodiment 70, wherein the at least one bacterium has a 16S nucleic acid sequences that shares at least 98% identity with SEQ ID NO: 6. 72. The agricultural composition of embodiment 70, wherein the at least one bacterium has a 16S nucleic acid sequences that shares at least 99% identity with SEQ ID NO: 6. 73. The agricultural composition of embodiment 70, wherein the at least one bacterium has a 16S nucleic acid comprising SEQ ID NO: 6. 74. The agricultural composition of embodiment 70, wherein the at least one bacterium is Mesorhizobium huakuii as deposited at NCMA 202504004. 75. The agricultural composition of any one of embodiments 70-74, wherein the microbial population further comprises a bacterium with a 16S nucleic acid sequence that shares at least 97% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1- 5 and 7-8. 76. The agricultural composition of any one of embodiments 1-75, wherein the microbial population is present in the composition at between 1×104to 1×1012cells per gram. 77. The agricultural composition of any one of embodiments 1-75, wherein the microbial population is present in the composition at between 1×106to 1×1012cells per gram. 78. The agricultural composition of any one of embodiments 1-75, wherein the microbial population is present in the composition at between 1×108to 1×1012cells per gram.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 79. The agricultural composition of any one of embodiments 1-78, wherein the composition is a liquid, and further comprises at least one metabolite produced by the at least one bacterium. 80. The agricultural composition of embodiment 79, wherein the at least one metabolite is selected from the group consisting of: bacteriocins, lipopeptides, polyketides, siderophores, enzymes, auxins, giberellins, cytokinins, pyrazines, indole, sulpher-containing compounds, organic acids, alcohols, and exopolysaccharides. 81. The agricultural composition of any one of embodiments 1-80, wherein the composition is a crop generalist soil inoculant. 82. The agricultural composition of any one of embodiments 1-81, wherein the agricultural composition is formulated as a seed coating, a fertilizer additive, a spray, a drench, or combinations thereof. 83. The agricultural composition of any one of embodiments 1-81, wherein the agricultural composition is encapsulated. 84. The agricultural composition of any one of embodiments 1-83, further comprising one or more of: a nutrient, a polymer, a salt, a sugar, a pesticide, a plant growth regulator, a beneficial agent, a biologically active agent, an herbicide, a bactericide, a fungicide, an insecticide, a virucide, a miticide, a nematocide, an acaricide, a rodenticide, an anti-algae agent, a biocontrol agent, another bacterial species, a fungal species, a compatibilizing agent, an antifoam agent, a cleaning agent, a sequestering agent, a drift reduction agent, a neutralizing agent, a buffer, a corrosion inhibitor, a dye, an odorant, a spreading agent, a penetration aid, a sticking agent, a binder, a thickening agent, an emulsifier, an antimicrobial agent, a fertilizer, an inert carrier, a polymer, and combinations thereof. 85. The agricultural composition of any one of embodiments 1-84, wherein the microbial population further comprises one or more of Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens. 86. A synthetic composition comprising a plant or a plant part, and the agricultural composition of any one of embodiments 1-85. 87. The synthetic composition of embodiment 86, comprising at least two isolated bacterial species. 88. The synthetic combination of embodiment 86 or 87, wherein the at least one bacterium is coated onto a seed, or applied onto the surface of a part of the plant, or applied to an area into which a plant will be planted, or combined with a fertilizer or other agricultural composition.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 89. A method of improving a marketable trait of a commodity plant product, comprising applying the agricultural composition of any one of embodiments 1-85 to a plant, plant part, or root zone. 90. The method of embodiment 89, wherein the improved marketable trait is an increase in plant biomass, compared to untreated plants. 91. The method of embodiment 89 or 90, wherein the commodity plant product is a fruit or vegetable. 92. The method of any one of embodiments 89-91, wherein the improved marketable trait is increased uniformity of a fruit or vegetable, compared to untreated plants. 93. The method of any one of embodiments 89-91, wherein the improved marketable trait is increased quality of a fruit or vegetable, compared to untreated plants. 94. The method of any one of embodiments 89-91, wherein the improved marketable trait is increased mass of a fruit or vegetable, compared to untreated plants. 95. The method of any one of embodiments 89-94, wherein the plant is a species from Solanaceae, Cannabaceae, Amaryllidaceous, Asteraceae, Solanum, Cucurbitaceae, Rutaceae, Rosaceae, Rubiaceae, Malvaceae, Musaceae, Poaceae, and Fabaceae. 96. The method of any one of embodiments 89-95, wherein the plant is a species from Lactuca, Zea, Triticum, Oryza, Solanum, Glycine, Brassica, Phaseolus, Cucurbita, Capsicum, Vitis, Citrus, Malus, Prunus, Coffea, Theobroma, Musa, Sorgham, Pisuym, Hordeum, and Cicer. 97. The method of any one of embodiments 89-96, wherein the commodity plant product is tomato fruit, lettuce, or bell pepper fruit. 98. The method of any one of embodiments 89-97, wherein the commodity plant product is tomato fruit, and application of the agricultural composition increases fruit uniformity between about 5% and about 25% compared to untreated plants. 99. The method of any one of embodiments 89-97, wherein the commodity plant product is tomato fruit, and application of the agricultural composition increases fruit quality between about 5% and about 25% compared to untreated plants. 100. The method of any one of embodiments 89-97, wherein the commodity plant product is tomato fruit, and application of the agricultural composition increases fruit mass between about 5% and about 25% compared to untreated plants. 101. The method of any one of embodiments 89-97, wherein the commodity plant product is tomato fruit, and the agricultural composition comprises Pseudomonas thivervalensis.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 102. The method of any one of embodiments 89-97, wherein the commodity plant product is tomato fruit, and the agricultural composition comprises Janibacter cremeus. 103. The method of any one of embodiments 89-97, wherein the commodity plant product is tomato fruit, and the agricultural composition comprises Mesorhozobium huakii. 104. The method of any one of embodiments 89-97, wherein the commodity plant product is tomato fruit, and the agricultural composition comprises Mesorhozobium huakii and Pseudomonas thivervalensis. 105. The method of any one of embodiments 89-97, wherein the commodity plant product is lettuce, and application of the agricultural composition increases plant biomass between about 5% and about 55% compared to untreated plants. 106. The method of any one of embodiments 89-97, wherein the commodity plant product is lettuce, and application of the agricultural composition increases head size between about 50% and about 200% compared to untreated control plants. 107. The method of any one of embodiments 89-97, wherein the commodity plant product is lettuce, and application of the agricultural composition increases quality between about 15% and about 80% compared to untreated control plants. 108. The method of any one of embodiments 89-97, wherein the commodity plant product is lettuce, and the agricultural composition comprises Janibacter cremeus. 109. The method of any one of embodiments 89-97, wherein the commodity plant product is lettuce, and the agricultural composition comprises Mesorhozobium huakii. 110. The method of any one of embodiments 89-97, wherein the commodity plant product is lettuce, and the agricultural composition comprises Pseudomonas thivervalensis. 111. The method of any one of embodiments 89-97, wherein the commodity plant product is lettuce, and the agricultural composition comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis. 112. The method of any one of embodiments 89-97, wherein the commodity plant product is lettuce, and the agricultural composition comprises Pseudomonas thivervalensis and Mesorhozobium huakii. 113. The method of any one of embodiments 89-97, wherein the commodity plant product is pepper fruit, and application of the agricultural composition increases fruit mass between about 5% and about 45% compared to untreated plants. 114. The method of any one of embodiments 89-97, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Mesorhozobium huakii.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 115. The method of any one of embodiments 89-97, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Pseudomonas thivervalensis. 116. The method of any one of embodiments 89-97, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis. 117. The method of any one of embodiments 89-97, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Pseudomonas thivervalensis and Mesorhozobium huakii. 118. The method of any one of embodiments 89-97, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Janibacter cremeus, Sphingomonas mali. 119. The method of any one of embodiments 89-118, wherein the method increases water use efficiency compared to untreated plants. 120. The method of any one of embodiments 89-119, wherein the composition is applied to plants under abiotic stress or prior to onset of abiotic stress. 121. The method of embodiment 120, wherein the abiotic stress is drought stress. 122. The method of any one of embodiments 89-121, wherein the composition is applied to two or more times. 123. The method of any one of embodiments 89-122, wherein the composition is applied to the rhizosphere. 124. A method for improving plant growth and / or increasing marketable yield, the method comprising: applying to a plant, plant part, or root zone at least one isolated bacterial species having a 16S nucleic acid sequence that shares at least 97% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8, and wherein the application of the at least one isolated bacterial species improves plant growth and / or increases marketable yield of the plant, compared to untreated plants. 125. The method of embodiment 124, wherein the at least one isolated bacterial species has a 16S nucleic acid sequence that shares at least 98% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8. 126. The method of embodiment 124, wherein the at least one isolated bacterial species has a 16S nucleic acid sequence that shares at least 99% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 127. The method of embodiment 124, wherein the at least one isolated bacterial species has a 16S nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8. 128. The method of any one of embodiments 124-127, wherein the at least one isolated bacterial species comprises Janibacter cremeus as deposited at NCMA 202504005. 129. The method of any one of embodiments 124-127, wherein the at least one isolated bacterial species comprises Sphingomonas mali as deposited at NCMA 202504005. 130. The method of any one of embodiments 124-127, wherein the at least one isolated bacterial species comprises Mesorhizobium ciceri as deposited at NCMA 202504005. 131. The method of any one of embodiments 124-127, wherein the at least one isolated bacterial species comprises Pseudomonas thivervalensis as deposited at NCMA 202504005. 132. The method of any one of embodiments 124-127, wherein the at least one isolated bacterial species comprises Sphingomonas oligoaromativorans as deposited at NCMA 202504005. 133. The method of any one of embodiments 124-127, wherein the at least one isolated bacterial species comprises Mesorhizobium huakuii as deposited at NCMA 202504004. 134. The method of any one of embodiments 124-127, wherein the at least one isolated bacterial species comprises Aeromicrobium ginsengisoli as deposited at NCMA 202504005. 135. The method of any one of embodiments 124-127, wherein the at least one isolated bacterial species comprises Sphingomonas melonis as deposited at NCMA 202504005. 136. The method of any one of embodiments 124-135, wherein the method comprises applying two isolated bacterial species. 137. The method of any one of embodiments 124-135, wherein the method comprises applying between three and five isolated bacterial species. 138. The method of any one of embodiments 124-135, wherein the method comprises applying between five and seven isolated bacterial species. 139. The method of any one of embodiments 124-135, wherein the method comprises applying seven or eight of the isolated bacterial species. 140. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Janibacter cremeus, and Sphingomonas mali. 141. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, and Pseudomonas thivervalensis.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 142. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Mesorhizobium huakuii. 143. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Aeromicrobium ginsengisoli. 144. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, Mesorhizobium huakuii, and Aeromicrobium ginsengisoli. 145. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, and Mesorhizobium huakuii. 146. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Pseudomonas thivervalensis and Mesorhizobium huakuii. 147. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, Aeromicrobium ginsengisoli, and Sphingomonas melonis. 148. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis. 149. The method of any one of embodiments 124-139, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Mesorhozobium huakii, and Sphingomonas melonis. 150. The method of any one of embodiments 124-149, further comprising applying one or more of Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens. 151. The method of any one of embodiments 124-150, wherein the plant is a species from Solanaceae, Cannabaceae, Amaryllidaceous, Asteraceae, Solanum, Cucurbitaceae, Rutaceae, Rosaceae, Rubiaceae, Malvaceae, Musaceae, Poaceae, and Fabaceae. 152. The method of any one of embodiments 124-151, wherein the plant is a species from Lactuca, Zea, Triticum, Oryza, Solanum, Glycine, Brassica, Phaseolus, Cucurbita, Capsicum,Attorney Docket No.: IMPE-005 / 02WO 342881-2039 Vitis, Citrus, Malus, Prunus, Coffea, Theobroma, Musa, Sorgham, Pisuym, Hordeum, and Cicer. 153. The method of any one of embodiments 124-152, wherein the at least one isolated bacterial species is lyophilized. 154. The method of any one of embodiments 124-152, wherein the at least one isolated bacterial species is encapsulated. 155. The method of any one of embodiments 124-154, wherein the at least one isolated bacterial species is applied to the soil prior to, or at the time of planting. 156. The method of any one of embodiments 124-155, wherein the at least one isolated bacterial species is applied to the rhizosphere. 157. The method of any one of embodiments 124-156, wherein the at least one isolated bacterial species is formulated as a solution, a wettable powder, a dusting powder, a soluble powder, an emulsion or suspension concentrate, a seed dressing or coating, a tablet, water- dispersible granules, water soluble granules, microencapsulated granules or suspensions, or as an irrigation component. 158. The method of any one of embodiments 124-157, wherein the applying step occurs by: coating a plant seed with the at least one isolated bacterial species, thereby resulting in a bacterial seed coating; coating a plant part with the at least one isolated bacterial species; spraying the at least one isolated bacterial species onto a plant part; spraying the at least one isolated bacterial species into a furrow into which a plant or seed will be placed; drenching the at least one isolated bacterial species onto a plant part or into an area into which a plant will be placed; spreading the at least one isolated bacterial species onto a plant part or into an area into which a plant will be placed; broadcasting the at least one isolated bacterial species onto a plant part or into an area into which a plant will be placed; or combining the at least one isolated bacterial species with a fertilizer or other agricultural composition that will be applied to a plant, plant part, or root zone. 159. The method of embodiment 158, wherein the bacterial seed coating about 1×103to about 1×1012bacterial cells per seed. 160. The method of any one of embodiments 124-158, wherein the at least one isolated bacterial species is applied at between 103and 1012colony forming units.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 161. The method of any one of embodiments 124-160, wherein the plant is under abiotic stress. 162. The method of any one of embodiments 124-161, wherein the plant is under drought stress. 163. A microbial composition comprising: a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 7; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 8, and an agriculturally acceptable carrier. 164. The microbial composition of embodiment 163, wherein said composition comprises: a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 3; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity SEQ ID NO: 5;Attorney Docket No.: IMPE-005 / 02WO 342881-2039 a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 6; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 7; and a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 8. 165. The microbial composition of embodiment 163, wherein said composition comprises: a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 3; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 5; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 6; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 7; and a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 8. 166. The microbial composition of embodiment 163, wherein said composition comprises: a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 3; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 5; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 6; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 7; and a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 8. 167. The microbial composition of embodiment 163, wherein said composition comprises:Attorney Docket No.: IMPE-005 / 02WO 342881-2039 Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhizobium huakuii, Aeromicrobium ginsengisoli, and Sphingomonas melonis, wherein the bacteria have been deposited at NCMA 202504005. 168. A microbial composition comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97- 99.9% identity with SEQ ID NO: 6; and an agriculturally acceptable carrier. 169. The microbial composition of embodiment 168, wherein the composition comprises at least one bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 6. 170. The microbial composition of embodiment 168, wherein the composition comprises Mesorhizobium huakuii as deposited at NCMA 202504004. 171. The microbial composition of any one of embodiments 168-170, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO: 1. 172. The microbial composition of any one of embodiments 168-171, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO: 2. 173. The microbial composition of any one of embodiments 168-172, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO: 3. 174. The microbial composition of any one of embodiments 168-173, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO: 4. 175. The microbial composition of any one of embodiments 168-174, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO: 5. The microbial composition of any one of embodiments 168-175, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO: 7. 176. The microbial composition of any one of embodiments 168-176, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO: 8.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 177. The microbial composition of any one of embodiments 168-176, further comprising at least one bacterium having a 16S nucleic acid sequence selected from SEQ ID NOs: 1-5 and 7-8. 178. The microbial composition of any one of embodiments 168-177, further comprising at least one bacterium selected from Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Aeromicrobium ginsengisoli, and Sphingomonas melonis, wherein the bacteria have been deposited at NCMA 202504005. 179. The microbial composition of any one of embodiments 163-178, wherein the composition is used in production of a liquid fertilizer or applied directly to a plant or plant part. 180. The microbial composition of any one of embodiments 163-179, wherein the bacteria are present in unequal ratios for the agricultural growth needs of a particular crop. 181. The microbial composition of any one of embodiments 163-180, wherein the bacteria are encapsulated. 182. The microbial composition of any one of embodiments 163-181, wherein the bacteria are lyophilized. 183. The microbial composition of any one of embodiments 163-182, further comprising one or more of Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens. 184. The microbial composition of any one of embodiments 163-183, further comprising one or more of: a nutrient, a polymer, a salt, a sugar, a pesticide, a plant growth regulator, a beneficial agent, a biologically active agent, an herbicide, a bactericide, a fungicide, an insecticide, a virucide, a miticide, a nematocide, an acaricide, a rodenticide, an anti-algae agent, a biocontrol agent, another bacterial species, a fungal species, a compatibilizing agent, an antifoam agent, a cleaning agent, a sequestering agent, a drift reduction agent, a neutralizing agent, a buffer, a corrosion inhibitor, a dye, an odorant, a spreading agent, a penetration aid, an adhesive agent, a binder, a thickening agent, an emulsifier, an antimicrobial agent, a fertilizer, an inert carrier, and combinations thereof. 185. The microbial composition of any one of embodiments 163-184, wherein the composition further comprises at least one metabolite produced by the at least one bacterium. 186. The microbial composition of any one of embodiments 163-185, wherein the composition is packaged as a kit comprising instructions for agricultural use.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 187. The kit of embodiment 186, wherein the microbial composition is formulated as a solution, a wettable powder, a dusting powder, a soluble powder, an emulsion or suspension concentrate, a seed dressing or coating, a tablet, water-dispersible granules, water soluble granules, microencapsulated granules or suspensions, or as an irrigation component.
Claims
Attorney Docket No.: IMPE-005 / 02WO 342881-2039 What is claimed is:
1. An agricultural composition comprising: a microbial population that comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the microbial population is present in the agricultural composition at between 1×103to 1×1012cells per gram.
2. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8.
3. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8.
4. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid selected from the group consisting of SEQ ID NOs: 1-8.
5. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
1.
6. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
1.
7. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
1.
8. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO:
1.
9. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 10. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
2.
11. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
2.
12. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO:
2.
13. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
3.
14. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
3.
15. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
3.
16. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO:
3.
17. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
4.
18. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
4.
19. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
4.
20. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO:
4.
21. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 22. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
5.
23. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
5.
24. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO:
5.
25. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
6.
26. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
6.
27. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
6.
28. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO:
6.
29. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
7.
30. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
7.
31. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
7.
32. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO:
7.
33. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 8.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 34. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
8.
35. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
8.
36. The agricultural composition of claim 1, wherein the microbial population comprises at least one bacterium with a 16S nucleic acid sequence comprising SEQ ID NO:
8.
37. The agricultural composition of claim 1, wherein the at least one bacterium is Janibacter cremeus as deposited at NCMA 202504005.
38. The agricultural composition of claim 1, wherein the at least one bacterium is Sphingomonas mali as deposited at NCMA 202504005.
39. The agricultural composition of claim 1, wherein the at least one bacterium is Mesorhizobium ciceri as deposited at NCMA 202504005.
40. The agricultural composition of claim 1, wherein the at least one bacterium is Pseudomonas thivervalensis as deposited at NCMA 202504005.
41. The agricultural composition of claim 1, wherein the at least one bacterium is Sphingomonas oligoaromativorans as deposited at NCMA 202504005.
42. The agricultural composition of claim 1, wherein the at least one bacterium is Mesorhizobium huakuii as deposited at NCMA 202504004.
43. The agricultural composition of claim 1, wherein the at least one bacterium is Aeromicrobium ginsengisoli as deposited at NCMA 202504005.
44. The agricultural composition of claim 1, wherein the at least one bacterium is Sphingomonas melonis as deposited at NCMA 202504005.
45. The agricultural composition of claim 1, wherein the at least one bacterium comprises two isolated bacterial species.
46. The agricultural composition of claim 1, wherein the at least one bacterium comprises between three and five isolated bacterial species.
47. The agricultural composition of claim 1, wherein the at least one bacterium comprises between five and seven isolated bacterial species.
48. The agricultural composition of claim 1, wherein the at least one bacterium comprises seven or eight isolated bacterial species.
49. The agricultural composition of claim 1, wherein the microbial population comprises:Attorney Docket No.: IMPE-005 / 02WO 342881-2039 at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
2.
50. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
4.
51. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
6.
52. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
7.
53. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2;Attorney Docket No.: IMPE-005 / 02WO 342881-2039 at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
7.
54. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
6.
55. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
6.
56. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 7; andAttorney Docket No.: IMPE-005 / 02WO 342881-2039 at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
8.
57. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
8.
58. The agricultural composition of claim 1, wherein the microbial population comprises: at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; and at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO:
8.
59. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, and Sphingomonas mali, said isolated bacterial species deposited at NCMA 202504005.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 60. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, and Pseudomonas thivervalensis, said isolated bacterial species deposited at NCMA 202504005.
61. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005.
62. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Aeromicrobium ginsengisoli, said isolated bacterial species deposited at NCMA 202504005.
63. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, Mesorhizobium huakuii, and Aeromicrobium ginsengisoli, said isolated bacterial species deposited at NCMA 202504005.
64. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005.
65. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Pseudomonas thivervalensis and Mesorhizobium huakuii, said isolated bacterial species deposited at NCMA 202504005.
66. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, Aeromicrobium ginsengisoli, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005.
67. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005.
68. The agricultural composition of claim 1, wherein the at least one bacterium comprises isolated bacterial species Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Mesorhozobium huakii, and Sphingomonas melonis, said isolated bacterial species deposited at NCMA 202504005.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 69. The agricultural composition of any one of claims 45-48, or 59-68, wherein each isolated bacterial species is present in the composition at approximately equal ratios.
70. An agricultural composition comprising: a microbial population that comprises at least one bacterium with a 16S nucleic acid sequence that shares at least 97% identity to SEQ ID NO: 6; and at least one of a stabilizer, a preservative, a dispersant, a wetting agent, and an agriculturally acceptable carrier, wherein the microbial population is present in the agricultural composition at between 1×103to 1×1012cells per gram.
71. The agricultural composition of claim 70, wherein the at least one bacterium has a 16S nucleic acid sequences that shares at least 98% identity with SEQ ID NO:
6.
72. The agricultural composition of claim 70, wherein the at least one bacterium has a 16S nucleic acid sequences that shares at least 99% identity with SEQ ID NO:
6.
73. The agricultural composition of claim 70, wherein the at least one bacterium has a 16S nucleic acid comprising SEQ ID NO:
6.
74. The agricultural composition of claim 70, wherein the at least one bacterium is Mesorhizobium huakuii as deposited at NCMA 202504004.
75. The agricultural composition of claim 70, wherein the microbial population further comprises a bacterium with a 16S nucleic acid sequence that shares at least 97% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-5 and 7-8.
76. The agricultural composition of claim 1, wherein the microbial population is present in the composition at between 1×104to 1×1012cells per gram.
77. The agricultural composition of claim 1, wherein the microbial population is present in the composition at between 1×106to 1×1012cells per gram.
78. The agricultural composition of claim 1, wherein the microbial population is present in the composition at between 1×108to 1×1012cells per gram.
79. The agricultural composition of claim 1, wherein the composition is a liquid, and further comprises at least one metabolite produced by the at least one bacterium.
80. The agricultural composition of claim 79, wherein the at least one metabolite is selected from the group consisting of: bacteriocins, lipopeptides, polyketides, siderophores, enzymes, auxins, giberellins, cytokinins, pyrazines, indole, sulpher-containing compounds, organic acids, alcohols, and exopolysaccharides.
81. The agricultural composition of claim 1, wherein the composition is a crop generalist soil inoculant.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 82. The agricultural composition of claim 1, wherein the agricultural composition is formulated as a seed coating, a fertilizer additive, a spray, a drench, or combinations thereof.
83. The agricultural composition of claim 1, wherein the agricultural composition is encapsulated.
84. The agricultural composition of claim 1, further comprising one or more of: a nutrient, a polymer, a salt, a sugar, a pesticide, a plant growth regulator, a beneficial agent, a biologically active agent, an herbicide, a bactericide, a fungicide, an insecticide, a virucide, a miticide, a nematocide, an acaricide, a rodenticide, an anti-algae agent, a biocontrol agent, another bacterial species, a fungal species, a compatibilizing agent, an antifoam agent, a cleaning agent, a sequestering agent, a drift reduction agent, a neutralizing agent, a buffer, a corrosion inhibitor, a dye, an odorant, a spreading agent, a penetration aid, a sticking agent, a binder, a thickening agent, an emulsifier, an antimicrobial agent, a fertilizer, an inert carrier, a polymer, and combinations thereof.
85. The agricultural composition of claim 1, wherein the microbial population further comprises one or more of Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens.
86. A synthetic composition comprising a plant or a plant part, and the agricultural composition of claim 1.
87. The synthetic composition of claim 86, comprising at least two isolated bacterial species.
88. The synthetic combination of claim 86, wherein the at least one bacterium is coated onto a seed, or applied onto the surface of a part of the plant, or applied to an area into which a plant will be planted, or combined with a fertilizer or other agricultural composition.
89. A method of improving a marketable trait of a commodity plant product, comprising applying the agricultural composition of claim 1 to a plant, plant part, or root zone.
90. The method of claim 89, wherein the improved marketable trait is an increase in plant biomass, compared to untreated plants.
91. The method of claim 89, wherein the commodity plant product is a fruit or vegetable.
92. The method of claim 89, wherein the improved marketable trait is increased uniformity of a fruit or vegetable, compared to untreated plants.
93. The method of claim 89, wherein the improved marketable trait is increased quality of a fruit or vegetable, compared to untreated plants.
94. The method of claim 89, wherein the improved marketable trait is increased mass of a fruit or vegetable, compared to untreated plants.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 95. The method of claim 89, wherein the plant is a species from Solanaceae, Cannabaceae, Amaryllidaceous, Asteraceae, Solanum, Cucurbitaceae, Rutaceae, Rosaceae, Rubiaceae, Malvaceae, Musaceae, Poaceae, and Fabaceae.
96. The method of claim 89, wherein the plant is a species from Lactuca, Zea, Triticum, Oryza, Solanum, Glycine, Brassica, Phaseolus, Cucurbita, Capsicum, Vitis, Citrus, Malus, Prunus, Coffea, Theobroma, Musa, Sorgham, Pisuym, Hordeum, and Cicer.
97. The method of claim 89, wherein the commodity plant product is tomato fruit, lettuce, or bell pepper fruit.
98. The method of claim 89, wherein the commodity plant product is tomato fruit, and application of the agricultural composition increases fruit uniformity between about 5% and about 25% compared to untreated plants.
99. The method of claim 89, wherein the commodity plant product is tomato fruit, and application of the agricultural composition increases fruit quality between about 5% and about 25% compared to untreated plants.
100. The method of claim 89, wherein the commodity plant product is tomato fruit, and application of the agricultural composition increases fruit mass between about 5% and about 25% compared to untreated plants.
101. The method of claim 89, wherein the commodity plant product is tomato fruit, and the agricultural composition comprises Pseudomonas thivervalensis.
102. The method of claim 89, wherein the commodity plant product is tomato fruit, and the agricultural composition comprises Janibacter cremeus.
103. The method of claim 89, wherein the commodity plant product is tomato fruit, and the agricultural composition comprises Mesorhozobium huakii.
104. The method of claim 89, wherein the commodity plant product is tomato fruit, and the agricultural composition comprises Mesorhozobium huakii and Pseudomonas thivervalensis.
105. The method of claim 89, wherein the commodity plant product is lettuce, and application of the agricultural composition increases plant biomass between about 5% and about 55% compared to untreated plants.
106. The method of claim 89, wherein the commodity plant product is lettuce, and application of the agricultural composition increases head size between about 50% and about 200% compared to untreated control plants.
107. The method of claim 89, wherein the commodity plant product is lettuce, and application of the agricultural composition increases quality between about 15% and about 80% compared to untreated control plants.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 108. The method of claim 89, wherein the commodity plant product is lettuce, and the agricultural composition comprises Janibacter cremeus.
109. The method of claim 89, wherein the commodity plant product is lettuce, and the agricultural composition comprises Mesorhozobium huakii.
110. The method of claim 89, wherein the commodity plant product is lettuce, and the agricultural composition comprises Pseudomonas thivervalensis.
111. The method of claim 89, wherein the commodity plant product is lettuce, and the agricultural composition comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis.
112. The method of claim 89, wherein the commodity plant product is lettuce, and the agricultural composition comprises Pseudomonas thivervalensis and Mesorhozobium huakii.
113. The method of claim 89, wherein the commodity plant product is pepper fruit, and application of the agricultural composition increases fruit mass between about 5% and about 45% compared to untreated plants.
114. The method of claim 89, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Mesorhozobium huakii.
115. The method of claim 89, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Pseudomonas thivervalensis.
116. The method of claim 89, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis.
117. The method of claim 89, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Pseudomonas thivervalensis and Mesorhozobium huakii.
118. The method of claim 89, wherein the commodity plant product is pepper fruit, and the agricultural composition comprises Janibacter cremeus, Sphingomonas mali.
119. The method of claim 89, wherein the method increases water use efficiency compared to untreated plants.
120. The method of claim 89, wherein the composition is applied to plants under abiotic stress or prior to onset of abiotic stress.
121. The method of claim 120, wherein the abiotic stress is drought stress.
122. The method of claim 89, wherein the composition is applied to two or more times.
123. The method of claim 89, wherein the composition is applied to the rhizosphere.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 124. A method for improving plant growth and / or increasing marketable yield, the method comprising: applying to a plant, plant part, or root zone at least one isolated bacterial species having a 16S nucleic acid sequence that shares at least 97% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8, and wherein the application of the at least one isolated bacterial species improves plant growth and / or increases marketable yield of the plant, compared to untreated plants.
125. The method of claim 124, wherein the at least one isolated bacterial species has a 16S nucleic acid sequence that shares at least 98% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8.
126. The method of claim 124, wherein the at least one isolated bacterial species has a 16S nucleic acid sequence that shares at least 99% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8.
127. The method of claim 124, wherein the at least one isolated bacterial species has a 16S nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-8.
128. The method of claim 124, wherein the at least one isolated bacterial species is Janibacter cremeus as deposited at NCMA 202504005.
129. The method of claim 124, wherein the at least one isolated bacterial species is Sphingomonas mali as deposited at NCMA 202504005.
130. The method of claim 124, wherein the at least one isolated bacterial species is Mesorhizobium ciceri as deposited at NCMA 202504005.
131. The method of claim 124, wherein the at least one isolated bacterial species is Pseudomonas thivervalensis as deposited at NCMA 202504005.
132. The method of claim 124, wherein the at least one isolated bacterial species is Sphingomonas oligoaromativorans as deposited at NCMA 202504005.
133. The method of claim 124, wherein the at least one isolated bacterial species is Mesorhizobium huakuii as deposited at NCMA 202504004.
134. The method of claim 124, wherein the at least one isolated bacterial species is Aeromicrobium ginsengisoli as deposited at NCMA 202504005.
135. The method of claim 124, wherein the at least one isolated bacterial species is Sphingomonas melonis as deposited at NCMA 202504005.
136. The method of claim 124, wherein the method comprises applying two isolated bacterial species.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 137. The method of claim 124, wherein the method comprises applying between three and five isolated bacterial species.
138. The method of claim 124, wherein the method comprises applying between five and seven isolated bacterial species.
139. The method of claim 124, wherein the method comprises applying seven or eight of the isolated bacterial species.
140. The method of claim 124, wherein the at least one isolated bacterial species comprises Janibacter cremeus, and Sphingomonas mali.
141. The method of claim 124, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, and Pseudomonas thivervalensis.
142. The method of claim 124, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Mesorhizobium huakuii.
143. The method of claim 124, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, and Aeromicrobium ginsengisoli.
144. The method of claim 124, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Pseudomonas thivervalensis, Mesorhizobium huakuii, and Aeromicrobium ginsengisoli.
145. The method of claim 124, wherein the at least one isolated bacterial species comprises Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, and Mesorhizobium huakuii.
146. The method of claim 124, wherein the at least one isolated bacterial species comprises Pseudomonas thivervalensis and Mesorhizobium huakuii.
147. The method of claim 124, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, Aeromicrobium ginsengisoli, and Sphingomonas melonis.
148. The method of claim 124, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhozobium huakii, and Sphingomonas melonis.
149. The method of claim 124, wherein the at least one isolated bacterial species comprises Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Mesorhozobium huakii, and Sphingomonas melonis.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 150. The method of claim 124, further comprising applying one or more of Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens.
151. The method of claim 124, wherein the plant is a species from Solanaceae, Cannabaceae, Amaryllidaceous, Asteraceae, Solanum, Cucurbitaceae, Rutaceae, Rosaceae, Rubiaceae, Malvaceae, Musaceae, Poaceae, and Fabaceae.
152. The method of claim 124, wherein the plant is a species from Lactuca, Zea, Triticum, Oryza, Solanum, Glycine, Brassica, Phaseolus, Cucurbita, Capsicum, Vitis, Citrus, Malus, Prunus, Coffea, Theobroma, Musa, Sorgham, Pisuym, Hordeum, and Cicer.
153. The method of claim 124, wherein the at least one isolated bacterial species is lyophilized.
154. The method of claim 124, wherein the at least one isolated bacterial species is encapsulated.
155. The method of claim 124, wherein the at least one isolated bacterial species is applied to the soil prior to, or at the time of planting.
156. The method of claim 124, wherein the at least one isolated bacterial species is applied to the rhizosphere.
157. The method of claim 124, wherein the at least one isolated bacterial species is formulated as a solution, a wettable powder, a dusting powder, a soluble powder, an emulsion or suspension concentrate, a seed dressing or coating, a tablet, water-dispersible granules, water soluble granules, microencapsulated granules or suspensions, or as an irrigation component.
158. The method of claim 124, wherein the applying step occurs by: coating a plant seed with the at least one isolated bacterial species, thereby resulting in a bacterial seed coating; coating a plant part with the at least one isolated bacterial species; spraying the at least one isolated bacterial species onto a plant part; spraying the at least one isolated bacterial species into a furrow into which a plant or seed will be placed; drenching the at least one isolated bacterial species onto a plant part or into an area into which a plant will be placed; spreading the at least one isolated bacterial species onto a plant part or into an area into which a plant will be placed; broadcasting the at least one isolated bacterial species onto a plant part or into an area into which a plant will be placed; orAttorney Docket No.: IMPE-005 / 02WO 342881-2039 combining the at least one isolated bacterial species with a fertilizer or other agricultural composition that will be applied to a plant, plant part, or root zone.
159. The method of claim 158, wherein the bacterial seed coating about 1×103to about 1×1012bacterial cells per seed.
160. The method of claim 124, wherein the at least one isolated bacterial species is applied at between 103and 1012colony forming units.
161. The method of claim 124, wherein the plant is under abiotic stress.
162. The method of claim 124, wherein the plant is under drought stress.
163. A microbial composition comprising: a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 3; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 5; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 6; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 7; a bacterium having a 16S nucleic acid sequence that shares at least 97% identity with SEQ ID NO: 8, and an agriculturally acceptable carrier.
164. The microbial composition of claim 163, wherein said composition comprises: a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 3;Attorney Docket No.: IMPE-005 / 02WO 342881-2039 a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity SEQ ID NO: 5; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 6; a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO: 7; and a bacterium having a 16S nucleic acid sequence that shares at least 98% identity with SEQ ID NO:
8.
165. The microbial composition of claim 163, wherein said composition comprises: a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 3; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 5; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 6; a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO: 7; and a bacterium having a 16S nucleic acid sequence that shares at least 99% identity with SEQ ID NO:
8.
166. The microbial composition of claim 163, wherein said composition comprises: a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 1; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 2; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 3; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 4; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 5; a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 6;Attorney Docket No.: IMPE-005 / 02WO 342881-2039 a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO: 7; and a bacterium having a 16S nucleic acid sequence comprising SEQ ID NO:
8.
167. The microbial composition of claim 163, wherein said composition comprises: Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Mesorhizobium huakuii, Aeromicrobium ginsengisoli, and Sphingomonas melonis, wherein the bacteria have been deposited at NCMA 202504005.
168. A microbial composition comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97- 99.9% identity with SEQ ID NO: 6; and an agriculturally acceptable carrier.
169. The microbial composition of claim 168, wherein the composition comprises at least one bacterium having a 16S nucleic acid sequence comprising SEQ ID NO:
6.
170. The microbial composition of claim 168, wherein the composition comprises Mesorhizobium huakuii as deposited at NCMA 202504004.
171. The microbial composition of claim 168, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO:
1.
172. The microbial composition of claim 168, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO:
2.
173. The microbial composition of claim 168, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO:
3.
174. The microbial composition of claim 168, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO:
4.
175. The microbial composition of claim 168, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO:
5. The microbial composition of claim 168, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO: 7.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 176. The microbial composition of claim 168, further comprising: at least one bacterium having a 16S nucleic acid sequence that shares between 97-99.9% identity with SEQ ID NO:
8.
177. The microbial composition of claim 168, further comprising at least one bacterium having a 16S nucleic acid sequence selected from SEQ ID NOs: 1-5 and 7-8.
178. The microbial composition of claim 168, further comprising at least one bacterium selected from Janibacter cremeus, Sphingomonas mali, Mesorhizobium ciceri, Pseudomonas thivervalensis, Sphingomonas oligoaromativorans, Aeromicrobium ginsengisoli, and Sphingomonas melonis, wherein the bacteria have been deposited at NCMA 202504005.
179. The microbial composition of any one of claims 163-178, wherein the composition is used in production of a liquid fertilizer or applied directly to a plant or plant part.
180. The microbial composition of any one of claims 163-179, wherein the bacteria are present in unequal ratios for the agricultural growth needs of a particular crop.
181. The microbial composition of any one of claims 163-180, wherein the bacteria are encapsulated.
182. The microbial composition of any one of claims 163-181, wherein the bacteria are lyophilized.
183. The microbial composition of any one of claims 163-182, further comprising one or more of Paenibacillus chitinolyticus, Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens.
184. The microbial composition of any one of claims 163-183, further comprising one or more of: a nutrient, a polymer, a salt, a sugar, a pesticide, a plant growth regulator, a beneficial agent, a biologically active agent, an herbicide, a bactericide, a fungicide, an insecticide, a virucide, a miticide, a nematocide, an acaricide, a rodenticide, an anti-algae agent, a biocontrol agent, another bacterial species, a fungal species, a compatibilizing agent, an antifoam agent, a cleaning agent, a sequestering agent, a drift reduction agent, a neutralizing agent, a buffer, a corrosion inhibitor, a dye, an odorant, a spreading agent, a penetration aid, an adhesive agent, a binder, a thickening agent, an emulsifier, an antimicrobial agent, a fertilizer, an inert carrier, and combinations thereof.
185. The microbial composition of any one of claims 163-184, wherein the composition further comprises at least one metabolite produced by the at least one bacterium.
186. The microbial composition of any one of claims 163-185, wherein the composition is packaged as a kit comprising instructions for agricultural use.Attorney Docket No.: IMPE-005 / 02WO 342881-2039 187. The kit of claim 186, wherein the microbial composition is formulated as a solution, a wettable powder, a dusting powder, a soluble powder, an emulsion or suspension concentrate, a seed dressing or coating, a tablet, water-dispersible granules, water soluble granules, microencapsulated granules or suspensions, or as an irrigation component.
Citation Information
Patent Citations
Use of non-Agrobacterium bacterial species for plant transformation
US10724042B2
Agriculturally beneficial microbes, microbial compositions, and consortia
US20230292764A1
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