Organic acid beverage that contains collagen peptide and fermented pitaya pulp and is capable of repairing joints and preparation method

Abstract

Disclosed in the present invention is an organic acid beverage that contains collagen peptide and fermented pitaya pulp and is capable of repairing joints, which organic acid beverage comprises the following components in percentages by weight: 25-40% of collagen peptide, 5-15% of fermented pitaya pulp, 8-24% of a food-medicine homologous extract, 4-10% of an acerola cherry powder and a compound nutritional fortifier, and 11-58% of an auxiliary material. Further disclosed in the present invention is a method for preparing an organic acid beverage that contains collagen peptide and fermented pitaya pulp and is capable of repairing joints. Provided in the present invention is an organic acid beverage that contains collagen peptide and fermented pitaya pulp and is capable of repairing joints, which organic acid beverage is formed by compounding a fermented pitaya pulp rich in short-chain fatty acids, a collagen peptide, a food-medicine homologous extract, an acerola cherry powder, a nutritional fortifier, etc., and has joint repair effects such as relieving joint pain, improving joint discomfort, and enhancing joint function.

Description

Collagen peptide dragon fruit original pulp fermented organic acid drink capable of repairing joints and preparation method thereof TECHNICAL FIELD

[0001] The present application relates to the technical field of collagen peptide drinks, in particular to a collagen peptide dragon fruit original pulp fermented organic acid drink capable of repairing joints and a preparation method thereof. BACKGROUND

[0002] Osteoarthritis is a chronic and progressive disease with high rates of disability and deformity worldwide, which has attracted widespread social attention. Its main pathological features include joint matrix disintegration, reduction of chondrocytes and hyperostosis. These symptoms not only seriously affect the daily life of patients, but also cause great pressure on the mental health of patients.

[0003] With the acceleration of population aging and the extension of life expectancy, the incidence of osteoarthritis is showing a trend of increasing year by year. However, it is worth noting that recent studies have shown that the impact of osteoarthritis on young people is also increasingly significant, which is not only due to the increase of factors such as sports injuries and overuse, but also related to the changes in modern lifestyle. Joint pain, stiffness and dysfunction caused by osteoarthritis seriously reduce the quality of life of patients, and even affect their work ability and social activities.

[0004] The pathological mechanism of osteoarthritis is complex, and the damage of articular cartilage is the core problem. Articular cartilage is mainly composed of chondrocytes and extracellular matrix, and chondrocytes are responsible for the synthesis and secretion of cartilage matrix and collagen fibers, which are crucial for the formation, metabolism and repair of cartilage. However, during the pathogenesis of osteoarthritis, chondrocytes undergo excessive apoptosis, which disrupts the balance between extracellular matrix synthesis and degradation, accelerating the progression of osteoarthritis.

[0005] Currently, the main approach for treating osteoarthritis drugs is to relieve pain, but it does not have any effect on the synthesis of intrinsic chondrocytes and matrix macromolecules. Although different alternative therapies have been applied to improve osteoarthritis, such as supplementing chondroitin sulfate and glucosamine, there are still deficiencies. Glucosamine interferes with glucose transport and insulin secretion, leading to hyperglycemia and insulin resistance. SUMMARY

[0006] In view of the above deficiencies in the prior art, the present application provides a collagen peptide dragon fruit original pulp fermented organic acid drink capable of repairing joints, which is formed by compounding collagen peptides, medicinal and edible extracts, needle cherry extracts and nutritional fortifying agents, etc. with dragon fruit fermented original pulp containing rich short-chain fatty acids, and has the effects of relieving joint pain, improving joint discomfort and improving joint function.

[0007] To achieve the above-mentioned purpose, the embodiments of the present application adopt the following technical solutions:

[0008] A collagen peptide dragon fruit original pulp fermented organic acid beverage capable of repairing joints comprises the following components in the weight percentage ratio: collagen peptide 25%-40%, dragon fruit fermented original pulp 5%-15%, medicinal and edible extract 8%-24%, needle leaf cherry powder and compound nutritional fortifier 4%-10%, and auxiliary material 11%-58%.

[0009] According to an aspect of the present application, the collagen peptide is prepared by microbial fermentation of type II collagen.

[0010] According to an aspect of the present application, the microbial fermentation agent used in the preparation of the collagen peptide comprises Bacillus, Enterobacter and Aeromonas.

[0011] According to an aspect of the present application, the dragon fruit fermented original pulp is prepared by blending red heart dragon fruit fermentation liquor rich in short-chain fatty acids and galactooligosaccharides, wherein the red heart dragon fruit fermentation liquor is obtained by a three-step directional fermentation method, namely aerobic and anaerobic fermentation, complete anaerobic fermentation and complete aerobic fermentation.

[0012] According to an aspect of the present application, the inoculated strain for the aerobic and anaerobic fermentation is yeast; the inoculated strain for the complete anaerobic fermentation is a microbial combined fermentation agent comprising Bifidobacterium, Bacteroides and Eubacterium; and the inoculated strain for the complete aerobic fermentation is acetic acid bacteria.

[0013] According to an aspect of the present application, the short-chain fatty acids in the dragon fruit fermented original pulp are not less than 11 kinds and the content of the short-chain fatty acids is ≥9%.

[0014] According to an aspect of the present application, the short-chain fatty acids include acetic acid, propionic acid and butyric acid, and the ratio among the acetic acid, propionic acid and butyric acid is 4:2:1.

[0015] According to an aspect of the present application, the medicinal and edible extract is one or more of extract of Polygonatum odoratum, extract of Crataegus pinnatifida and extract of Ganoderma lucidum.

[0016] According to an aspect of the present application, the auxiliary material is one or more of drinking water, potassium sorbate and sucralose.

[0017] According to an aspect of the present application, the compound nutritional fortifier is nicotinamide, vitamin B6, vitamin B12 and folic acid.

[0018] A preparation method of a collagen peptide dragon fruit original pulp fermented organic acid beverage capable of repairing joints comprises the following steps:

[0019] Collagen peptide, medicinal and edible extract, pitaya fermentation original pulp, needle leaf cherry extract and compound nutritional fortifier, excipient are weighed according to the proportion of weight fraction, mixed and stirred until the material is completely dissolved, defoamed to obtain a collagen peptide pitaya original pulp fermentation organic acid beverage capable of repairing joints.

[0020] According to one aspect of the present application, the defoaming further comprises filling and sterilization.

[0021] The collagen peptide is made of type II collagen fermented by microbial fermentation method, and has a molecular weight less than 1000 Dalton, and is easy to be absorbed by human bodies. Type II collagen is the main collagen of cartilage, has the function of promoting chondrocyte differentiation, and together with a small amount of other collagens, forms a three-dimensional fiber structure, which is very important for the hardness and tensile strength of cartilage. Therefore, the collagen peptide can promote the differentiation of chondrocytes, reduce the loss and damage of cartilage structure, maintain the balance of cartilage extracellular matrix synthesis and degradation, thereby preventing or delaying the progression of osteoarthritis, so as to achieve the purpose of repairing joints. The fermented original pulp of dragon fruit can improve the bad flavor of the collagen peptide on the one hand, and the core component of short-chain fatty acids plays an important role in the body's immunity, metabolism and endocrine, etc. on the other hand. The intervention of short-chain fatty acids helps to restore the balance of normal intestinal flora, improve intestinal flora disorder, and also regulates B cell differentiation through FFAR2, thereby helping to alleviate rheumatoid arthritis. Short-chain fatty acids are also involved in the process of bone and joint repair. First, short-chain fatty acids can reduce joint damage and pain by inhibiting inflammatory response and oxidative stress. Second, short-chain fatty acids can promote the proliferation and differentiation of articular chondrocytes, thereby helping to repair and regenerate articular cartilage. In addition, short-chain fatty acids can also regulate bone metabolism, promote bone formation and mineralization, thereby helping to heal bone fractures and increase bone density. The combination of short-chain fatty acids and collagen can not only improve intestinal function and promote intestinal health, but also indirectly promote the absorption and utilization of collagen peptides when the intestinal mucosal cells' nutrient uptake capacity is improved. On the other hand, it can effectively prevent the increase of intestinal permeability, thereby inhibiting the large number of intestinal microflora and their metabolites from entering the blood circulation, and avoiding the initiation of inflammation. The combination of short-chain fatty acids and collagen can also promote metabolism by restoring the balance of intestinal flora, enhance the absorption and bioavailability of collagen peptides, thereby promoting the differentiation of chondrocytes and the repair of articular cartilage, and improving the efficiency of collagen peptides in relieving joint pain, improving joint discomfort and enhancing joint function. The amino acids contained in polygonatum odoratum are more than 20, of which 7 are essential amino acids and 2 are semi-essential amino acids. The active ingredients in polygonatum odoratum extract mainly include flavonoids, polysaccharides, saponins, volatile oils, alkaloids, trace elements and lectins, etc. Therefore, polygonatum odoratum has the functions of strengthening heart, lowering blood pressure, lowering blood sugar, lowering blood lipids, antioxidant, anti-tumor and enhancing immunity, etc. and many other benefits to the human body. The extract of hawthorn contains a variety of vitamins and rich antioxidant polyphenols and flavonoids. These compounds can reduce the levels of total cholesterol, low-density lipoprotein cholesterol and triglycerides, and have the ability to scavenge free radicals DPPH. At the same time, the hyperoside in hawthorn extract can inhibit the proliferation of synoviocytes and has significant anti-infection effect.Ganoderma lucidum extract contains various active substances such as amino acids, proteins, vitamins, polysaccharides, glycosides, alkaloids, sterols, anthraquinones, flavonoids and antibiotics, etc., has the effects of protecting liver, antioxidant, anti-aging, enhancing immunity, etc., among which its effects of reducing the production of pro-inflammatory cytokines and protecting chondrocytes are of great significance to the improvement of cartilage diseases such as arthritis. Therefore, the collagen peptide pitaya original pulp fermented organic acid beverage has the effects of relieving joint pain, improving joint discomfort, improving joint function and repairing joints. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0023] A collagen peptide pitaya original pulp fermented organic acid beverage capable of repairing joints comprises the following components in the weight ratio: collagen peptide 25%-40%, pitaya fermented original pulp 5%-15%, medicinal and edible extract 8%-24%, needle cherry powder and compound nutritional fortifier 4%-10%, and auxiliary material 11%-58%.

[0024] In actual application, the collagen peptide is type II collagen peptide, which is prepared by fermentation with a microbial combined starter. The microbial combined starter comprises Bacillus, Enterobacter and Aeromonas. The preparation method of the type II collagen peptide is as follows: the animal cartilage is pretreated by cleaning, crushing, sterilization and the like, then mixed with the microbial combined preparation culture liquid after activation, screening and large-scale culture to perform fermentation and enzymatic hydrolysis, and after the fermentation is completed, the collagen peptide in the enzymatic hydrolysis liquid is separated from other impurities through centrifugation, filtration and the like, and then purified, and finally the purified collagen peptide is concentrated and dried to obtain the type II collagen peptide. The type II collagen peptide has a molecular weight less than 1000 Dalton, and is easy to be absorbed by the human body. Type II collagen is the main collagen of cartilage, accounting for 90%-95% of cartilage collagen, and therefore, type II collagen is considered as the characteristic collagen of cartilage, and has the function of promoting chondrocyte differentiation. Meanwhile, type II collagen forms a three-dimensional fiber structure together with other small amount of collagen, which is very important to the hardness and tensile strength of cartilage.

[0025] In practical applications, the pitaya fermentation raw pulp takes the new plant identified variety "Mogu No. 1" red heart pitaya bred from the company's own thousands of mu pitaya planting base as the fermentation raw material, and is prepared by three-step directional fermentation method using a special "13259" fermentation process, as shown in CN201810157407.3. Specifically, the pitaya fermentation raw pulp is prepared by the following method: the pitaya fermentation raw pulp is prepared by blending red heart pitaya fermentation broth rich in short-chain fatty acids and galactooligosaccharides at a weight ratio of 10:3-6. The red heart pitaya fermentation broth is obtained by three-step directional fermentation method, and the three-step directional fermentation method is aerobic and anaerobic fermentation, complete anaerobic fermentation, and complete aerobic fermentation in turn. The aerobic and anaerobic fermentation is to add 4-7 parts of brown sugar to 100 parts of crushed red heart pitaya pulp by weight, the temperature is controlled at 25-30℃, and the law of yeast as the initial fermentation dominant bacteria is followed, and timely ventilation or non-ventilation management is performed. The complete anaerobic fermentation is to add 4-8 parts of brown sugar and inoculate 0.7-1.5 parts of microbial combined fermentation agent when the yeast activity is weak and spores are formed, the temperature is controlled at 30-35℃, and complete anaerobic fermentation is carried out until the 38th-45th day to produce a large amount of short-chain fatty acids. Among them, the microbial combined fermentation agent is mainly composed of Bifidobacterium, Bacteroides, and Eubacterium, and the total number of microorganisms in the microbial combined fermentation agent is ≥8×10cfu / g by plate counting method. The complete aerobic fermentation is to inoculate 0.4-0.7 parts of acetic acid bacteria when the pH value no longer changes, and the temperature is controlled at 28-35℃ to convert ethanol to acetic acid, and the filtrate is obtained by filtration. Further, the filtrate is ripened, further oxidized and clarified until the mass fraction content of ethanol in the fermentation product is less than 0.2%, and the red heart pitaya fermentation broth is obtained. The three-step directional fermentation method enriches the content of short-chain fatty acids in the red heart pitaya fermentation broth.

[0026] In practical applications, the red heart pitaya fermentation broth contains the nutrients of red heart pitaya itself, and a large amount of short-chain fatty acids obtained by fermentation of yeast, microbial combined fermentation agent, and acetic acid bacteria. Functional galactooligosaccharides are fermented by anaerobic bacteria to produce short-chain fatty acids in the colon.

[0027] In practical applications, the pitaya fermentation raw pulp contains not less than 11 kinds of short-chain fatty acids and the content of short-chain fatty acids is ≥9%, including but not limited to acetic acid, propionic acid, butyric acid, adipic acid, lactic acid, malic acid, pyruvic acid, citric acid, tartaric acid, fumaric acid, and maleic acid. Among them, the main short-chain fatty acids are acetic acid, propionic acid, and butyric acid, and the ratio among acetic acid, propionic acid, and butyric acid is 4:2:1.

[0028] In practical applications, the pitaya fermented raw pulp contains rich short-chain fatty acids. The intervention of short-chain fatty acids helps to restore the balance of normal intestinal flora, improve intestinal flora disorder, and also helps to alleviate rheumatoid arthritis by regulating B cell differentiation through FFAR2. Short-chain fatty acids are also involved in the process of bone and joint repair. First, short-chain fatty acids can reduce joint damage and pain by inhibiting inflammatory response and oxidative stress. Second, short-chain fatty acids can promote the proliferation and differentiation of articular chondrocytes, thereby helping to repair and regenerate articular cartilage. In addition, short-chain fatty acids can also regulate bone metabolism, promote bone formation and mineralization, thereby helping to heal bone fractures and increase bone density. Therefore, short-chain fatty acids play an important role in bone and joint repair.

[0029] In practical applications, the medicinal and edible extract is one or more of polygonatum extract, hawthorn extract, and ganoderma extract. Polygonatum contains more than 20 kinds of amino acids, including 7 kinds of essential amino acids and 2 kinds of semi-essential amino acids. The active ingredients in polygonatum extract mainly include flavonoids, polysaccharides, saponins, volatile oils, alkaloids, trace elements, and lectins, etc. Therefore, polygonatum has the functions of strengthening heart, lowering blood pressure, lowering blood sugar, lowering blood lipids, antioxidant, anti-tumor, and enhancing immunity, etc. and many benefits to the human body. Hawthorn extract contains various vitamins and rich polyphenolic compounds and flavonoids with strong antioxidant properties. These compounds can reduce the levels of total cholesterol, low-density lipoprotein cholesterol, and triglycerides, and have the ability to scavenge free radicals DPPH. At the same time, the hyperoside in hawthorn extract can inhibit the proliferation of synoviocytes and has significant anti-infection effect. Ganoderma extract contains various active substances such as amino acids, proteins, vitamins, polysaccharides, glycosides, alkaloids, sterols, anthraquinones, flavonoids, and antibiotics, etc. It has the effects of protecting liver, antioxidant, anti-aging, and enhancing immunity, etc. Among them, its role in reducing the production of pro-inflammatory cytokines and protecting chondrocytes is of great significance for the improvement of cartilage diseases such as arthritis.

[0030] In practical applications, the complex nutritional fortifier is nicotinamide, vitamin B6, vitamin B12, and folic acid.

[0031] In practical applications, the auxiliary material is one or more of drinking water, potassium sorbate, and sucralose.

[0032] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage capable of repairing joints comprises the following steps: weighing collagen peptide, medicinal and edible extract, dragon fruit fermented original pulp, needle leaf cherry extract and compound nutritional fortifier and auxiliary materials according to the weight fraction ratio, mixing and stirring the above-mentioned raw materials in a reaction container until the materials are completely dissolved, stirring the dissolved solution uniformly, then standing and defoaming, filling after becoming a homogeneous liquid, sterilizing after filling is completed, and obtaining the collagen peptide dragon fruit original pulp fermented organic acid beverage capable of repairing joints.

[0033] Example one

[0034] The collagen peptide dragon fruit original pulp fermented organic acid beverage comprises the following weight fraction ratio of components: collagen peptide 35%, dragon fruit fermented original pulp 12%, polygonatum extract 8%, hawthorn extract 8%, ganoderma extract 2%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 29.96%, sucralose 0.01% and potassium sorbate 0.02%.

[0035] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage of the embodiment is as follows: weighing each component raw material according to the weight fraction ratio, mixing and stirring the above-mentioned raw materials in a reaction tank until the materials are completely dissolved, stirring the dissolved solution uniformly, then standing, filling after becoming a homogeneous liquid, sterilizing after filling is completed, and obtaining the collagen peptide dragon fruit original pulp fermented organic acid beverage.

[0036] Example two

[0037] The collagen peptide dragon fruit original pulp fermented organic acid beverage comprises the following weight fraction ratio of components: collagen peptide 35%, dragon fruit fermented original pulp 12%, polygonatum extract 8%, hawthorn extract 8%, ganoderma extract 2%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 29.96%, sucralose 0.01% and potassium sorbate 0.02%.

[0038] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided in the embodiment is the same as that in example one.

[0039] Example three

[0040] The collagen peptide dragon fruit original pulp fermented organic acid beverage comprises the following weight fraction ratio of components: collagen peptide 30%, dragon fruit fermented original pulp 8%, polygonatum extract 8%, hawthorn extract 8%, ganoderma extract 2%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 38.96%, sucralose 0.01% and potassium sorbate 0.02%.

[0041] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided in this embodiment is the same as that in Embodiment 1.

[0042] Embodiment Four

[0043] A collagen peptide dragon fruit original pulp fermented organic acid beverage comprises the following components in the weight percentage ratio: collagen peptide 30%, dragon fruit fermented original pulp 8%, polygonatum extract 5%, hawthorn extract 5%, ganoderma extract 1%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 45.96%, sucralose 0.01%, and potassium sorbate 0.02%.

[0044] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided in this embodiment is the same as that in Embodiment 1.

[0045] Comparative Example One

[0046] A collagen peptide dragon fruit original pulp fermented organic acid beverage comprises the following components in the weight percentage ratio: collagen peptide 35%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 59.96%, sucralose 0.01%, and potassium sorbate 0.02%.

[0047] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided in this embodiment is the same as that in Embodiment 1.

[0048] Comparative Example Two

[0049] A collagen peptide dragon fruit original pulp fermented organic acid beverage comprises the following components in the weight percentage ratio: dragon fruit fermented original pulp 12%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 82.96%, sucralose 0.01%, and potassium sorbate 0.02%.

[0050] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided in this embodiment is the same as that in Embodiment 1.

[0051] Comparative Example Three

[0052] A collagen peptide dragon fruit original pulp fermented organic acid beverage comprises the following components in the weight percentage ratio: polygonatum extract 8%, hawthorn extract 8%, ganoderma extract 2%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 76.96%, sucralose 0.01%, and potassium sorbate 0.02%.

[0053] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided in this embodiment is the same as that in Embodiment 1.

[0054] Comparative Example Four

[0055] A collagen peptide dragon fruit original pulp fermented organic acid beverage, comprising the following components in weight percentage: collagen peptide 35%, dragon fruit fermented original pulp 12%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 47.96%, sucralose 0.01%, potassium sorbate 0.02%.

[0056] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided by the present comparative example is the same as that of Example 1.

[0057] Comparative Example 5

[0058] A collagen peptide dragon fruit original pulp fermented organic acid beverage, comprising the following components in weight percentage: collagen peptide 35%, polygonatum extract 8%, hawthorn extract 8%, ganoderma extract 2%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 41.96%, sucralose 0.01%, potassium sorbate 0.02%.

[0059] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided by the present comparative example is the same as that of Example 1.

[0060] Comparative Example 6

[0061] A collagen peptide dragon fruit original pulp fermented organic acid beverage, comprising the following components in weight percentage: dragon fruit fermented original pulp 12%, polygonatum extract 8%, hawthorn extract 8%, ganoderma extract 2%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 64.96%, sucralose 0.01%, potassium sorbate 0.02%.

[0062] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided by the present comparative example is the same as that of Example 1.

[0063] Comparative Example 7

[0064] A collagen peptide dragon fruit original pulp fermented organic acid beverage, comprising the following components in weight percentage: collagen peptide 20%, dragon fruit fermented original pulp 4%, polygonatum extract 3%, hawthorn extract 3%, ganoderma extract 1%, needle leaf cherry extract 3%, compound nutritional fortifier 0.01%, drinking water 65.96%, sucralose 0.01%, potassium sorbate 0.02%.

[0065] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided by the present comparative example is the same as that of Example 1.

[0066] Comparative Example 8

[0067] A collagen peptide dragon fruit original pulp fermented organic acid beverage comprises the following components in weight percentage ratio: collagen peptide 35%, dragon fruit fermented original pulp 12% (the dragon fruit used in the dragon fruit fermented original pulp is a common dragon fruit on the market, not the new plant identified variety "Mogu No. 1" red heart dragon fruit bred by the company's own thousand-mu dragon fruit planting base, and the other preparation steps are consistent with the preparation steps of the dragon fruit fermented original pulp in the present application), polygonatum extract 8%, hawthorn extract 8%, ganoderma lucidum extract 2%, needle leaf cherry extract 5%, compound nutritional fortifier 0.01%, drinking water 29.96%, sucralose 0.01%, and potassium sorbate 0.02%.

[0068] The preparation method of the collagen peptide dragon fruit original pulp fermented organic acid beverage provided by the present application is the same as that of Example 1.

[0069] Effect verification example

[0070] The WOMAC score is used to monitor the severity of arthritis and its treatment effect by 24 parameters of pain, stiffness and joint function, and the score range is 0-96 points. The lower the score, the better the effect. The Lysholm score is composed of 8 parameters, such as lameness, support, locking, instability, pain, swelling, climbing stairs, and squatting, and the score range is 0-100 points. It can effectively evaluate the symptoms and function of the knee joint, and the higher the score, the better the effect.

[0071] Take the beverage prepared in Example 1, and take 80 patients with bone joint pain, rheumatoid arthritis or joint discomfort as experimental objects. The patients have not taken any drugs or supplements related to the treatment of joint pain for three months before the test and during the test period. The subjects are randomly divided into two groups: the drinking group and the control group, with 40 people in each group. The drinking group drinks one serving of the beverage prepared in Example 1 per day, and the control group drinks one serving of malt dextrin solution (malt dextrin weight percentage ratio 35%) of the same quality per day. The subjects fill out the questionnaire before drinking, and the WOMAC score and Lysholm score are determined according to the clinical symptoms and questionnaire survey. After continuous drinking for three months, the scores are determined again. The collected data are statistically analyzed by SPSS 27.0, and the changes of the indicators of the two groups before and after drinking and the differences in the change trend within the groups before and after drinking are compared. P<0.05 indicates that the difference is statistically significant, and the results are shown in Tables 1 and 2.

[0072] Table 1 Comparison of WOMAC and Lysholm scores before and after drinking in the two groups (x±s)

[0073]

[0074] Table 2 Comparison of WOMAC and Lysholm scores before and after drinking in the two groups (x±s)

[0075]

[0076] As shown in Table 1, the difference in WOMAC score and Lysholm score of the drinking group before and after drinking for three months was significant, and the change trend was statistically significant (P<0.001), while the difference in WOMAC score and Lysholm score of the control group before and after drinking for three months was not statistically significant.

[0077] As shown in Table 2, t-test was used to compare the difference in WOMAC score of the two groups of subjects before and after drinking collagen peptide and malt dextrin, and it was found that the change trend of the subjects' WOMAC score was different between the two groups before and after drinking, and the decrease in the drinking group was more obvious, and the change trend was statistically significant (P<0.001). It was found that there was a difference in WOMAC score in the drinking group before and after drinking for three months (P<0.001), indicating that drinking collagen peptide and pitaya original pulp fermented organic acid drinks could more effectively reduce the WOMAC score than malt dextrin, and had a certain help for the repair of osteoarthritis of the subjects.

[0078] At the same time, t-test was also used to compare the difference in Lysholm score of the two groups of subjects before and after drinking collagen peptide and pitaya original pulp fermented organic acid drinks and malt dextrin, and it was found that the change trend of the subjects' Lysholm score was different between the two groups before and after drinking, and the increase in the drinking group was more obvious, and the change trend was statistically significant (P<0.001). It was found that there was a difference in Lysholm score in the drinking group before and after drinking for three months (P<0.001), indicating that drinking collagen peptide could more effectively increase the Lysholm score than malt dextrin, and had a certain help for the repair of osteoarthritis of the subjects.

[0079] The drinks prepared in Example 1, Example 2, Example 3, Example 4, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7, Comparative Example 8 were used as experimental objects for 240 patients who complained of bone joint pain, rheumatoid arthritis or joint discomfort. The patients did not take any drugs or supplements related to joint pain for three months before the experiment and during the experiment. The subjects were randomly divided into Test Group 1, Test Group 2, Test Group 3, Test Group 4, Control Group 1, Control Group 2, Control Group 3, Control Group 4, Control Group 5, Control Group 6, Control Group 7, Control Group 8, a total of twelve groups, 20 people in each group. Each group in the test group drank the corresponding drinks prepared in Example 1 to Example 4 every day, and each group in the control group drank the corresponding drinks prepared in Comparative Example 1 to Comparative Example 8 every day. The subjects filled out a questionnaire before drinking, and the WOMAC score and Lysholm score were determined according to the clinical symptoms and questionnaire survey. After continuous drinking for three months, the scores were determined again. The average values and average value difference changes of the WOMAC scores and Lysholm scores of the twelve groups of subjects before and after drinking were compared, and the results are shown in Table 3.

[0080] Table 3

[0081]

[0082] As can be seen from Table 3, the joint discomfort of the subjects in Test Groups 1-4 was improved, and the improvement effect of the subjects in Test Group 1 was the most obvious, indicating that Example 1 is the optimal combination and has the best effect on improving arthritis. By comparing Test Group 1 with Test Group 3 and Test Group 2 with Test Group 4, it can be seen that when the weight fraction ratio of the medicinal and edible extract is the same, the greater the weight fraction ratio of collagen peptide and dragon fruit fermented raw pulp, the more obvious the improvement effect of the collagen peptide and dragon fruit raw pulp fermented organic acid drink on the joint. By comparing Test Group 1 with Test Group 2 and Test Group 3 with Test Group 4, it can be seen that when the weight fraction ratio of collagen peptide and dragon fruit fermented raw pulp is the same, as the weight fraction ratio of medicinal and edible extract increases, the improvement effect of the collagen peptide and dragon fruit raw pulp fermented organic acid drink on the joint also increases, but the improvement effect is far less than that when the weight fraction ratio of collagen peptide and dragon fruit fermented raw pulp increases, indicating that the improvement of the collagen peptide and dragon fruit raw pulp fermented organic acid drink on the joint is mainly due to collagen peptide and dragon fruit fermented raw pulp.

[0083] In addition, comparing test group 1 and control groups 1-6, the improvement effect on joint discomfort: test group 1 > control group 4 > control group 6 > control group 5 > control group 2 > control group 1 > control group 3, it is illustrated that the pitaya fermented original pulp, the medicine-food homologous extract and the collagen peptide have a synergistic effect on joint discomfort, and the synergistic effect of the pitaya fermented original pulp and the collagen peptide is obviously better than that of the medicine-food homologous extract and the collagen peptide, and the synergistic effect of the collagen peptide, the pitaya fermented original pulp and the medicine-food homologous extract is the best, these components complement each other, and the joint discomfort is improved to the best when they are combined scientifically.

[0084] Comparing test group 1 and control group 7, the joint discomfort of the subjects in control group 7 is improved to a certain extent, but the improvement effect is obviously lower than that of test group 1 and test groups 2-4, so it is illustrated that when the weight part of the composition provided by the application is not in a specific range, even if the components are the same, the improvement effect on joint discomfort will be greatly reduced.

[0085] Comparing test group 1 and control group 8, the joint discomfort of the subjects in control group 8 is improved to a certain extent, but the improvement effect is obviously lower than that of test group 1 and test groups 2-4, and is similar to that of control group 5, so it can be seen that the collagen peptide and the medicine-food homologous extract in control group 8 mainly improve the joint, rather than the pitaya fermented original pulp (the pitaya used in the pitaya fermented original pulp is a common pitaya on the market, not a new plant identified variety "Mogu No. 1" red heart pitaya selected by the company's own thousand-mu pitaya planting base). It is illustrated that the short-chain fatty acid species and content contained in the pitaya fermented original pulp provided by the application are much higher than those of the pitaya fermented original pulp made of common pitaya on the market, and the improvement effect on joint discomfort is also much higher than that of the pitaya fermented original pulp made of common pitaya on the market.

[0086] From the above, it can be seen that the drink provided by the application has the joint repair effects of relieving joint pain, improving joint discomfort and improving joint function.

[0087] The collagen peptide is made of type II collagen fermented by microbial fermentation method, and has a molecular weight less than 1000 Dalton, which is easy to be absorbed by human body. Type II collagen is the main collagen of cartilage, has the function of promoting chondrocyte differentiation, and together with other small amount of collagen, forms a three-dimensional fiber structure, which is very important for the hardness and tensile strength of cartilage. Therefore, the collagen peptide can promote the differentiation of chondrocytes, reduce the loss and damage of cartilage structure, maintain the balance of cartilage extracellular matrix synthesis and degradation, thereby preventing or delaying the progression of osteoarthritis, so as to achieve the purpose of repairing joints. The fermented original pulp of dragon fruit can improve the bad flavor of collagen peptide on the one hand; on the other hand, the core component of short-chain fatty acids can balance the three major basic metabolisms of human body, promote the growth of intestinal mucosal cells, regulate the balance of intestinal flora, improve intestinal function, and is a bridge for intestinal flora to play an important role. Therefore, the intervention of short-chain fatty acids helps to restore the balance of normal intestinal flora, improve intestinal flora disorder, effectively prevent the occurrence and development of rheumatoid arthritis, and even achieve the purpose of cure. In addition, short-chain fatty acids have been considered as a key link between microorganisms and bone tissue. As the three main components of short-chain fatty acids, acetic acid, propionic acid and butyric acid have a certain regulatory effect on bone metabolism. The combination of short-chain fatty acids and collagen peptides not only improves the intestinal function, promotes intestinal health, effectively prevents the increase of intestinal permeability, thereby inhibiting the large number of proliferation of intestinal microflora and its metabolites into the blood circulation, avoiding the initiation of inflammation; but also promotes biological metabolism by restoring the balance of intestinal flora through short-chain fatty acids, thereby enhancing the absorption of collagen peptides and the bioavailability of collagen peptides, promoting the differentiation of chondrocytes and the repair of articular cartilage, and improving the conversion efficiency of collagen peptides in repairing joints. There are more than 20 kinds of amino acids in polygonatum, including 7 kinds of essential amino acids and 2 kinds of semi-essential amino acids. The active ingredients in polygonatum extract mainly include flavonoids, polysaccharides, saponins, volatile oil, alkaloids, trace elements and agglutinin compounds, etc. Therefore, polygonatum has the functions of strengthening heart, lowering blood pressure, lowering blood sugar, lowering blood lipid, antioxidant, anti-tumor and enhancing immunity, and many other benefits to the human body. The hawthorn extract contains a variety of vitamins and rich antioxidant polyphenols and flavonoids. These compounds can reduce the levels of total cholesterol, low-density lipoprotein cholesterol and triglycerides, and have the ability to scavenge free radicals DPPH; at the same time, the hyperoside in hawthorn extract can inhibit the proliferation of synoviocytes and has significant anti-infection effect. The ganoderma extract contains various active substances such as amino acids, proteins, vitamins, polysaccharides, glycosides, alkaloids, sterols, anthraquinones, flavonoids and antibiotics, etc., and has the functions of protecting liver, antioxidant, anti-aging, enhancing immunity, etc. Among them, its role in reducing the production of pro-inflammatory cytokines and protecting chondrocytes is of great significance to the improvement of joint diseases such as arthritis.Therefore, the beverage provided by the application has the effects of relieving joint pain, improving joint discomfort, improving joint function, and the like.

[0088] The above merely provides a specific implementation of the application, but the protection scope of the application is not limited thereto, any person skilled in the art can easily think of changes or replacements within the technical scope disclosed by the application, which should be covered in the protection scope of the application. Therefore, the protection scope of the application should be subject to the protection scope of the claims.

Claims

1. A collagen peptide-based fermented organic acid beverage made from dragon fruit pulp that can repair joints, characterized in that... It includes the following components in the following weight percentages: collagen peptides 25%-40%, dragon fruit fermented pulp 5%-15%, medicinal and edible extracts 8%-24%, acerola cherry powder and compound nutritional fortifiers 4%-10%, and excipients 11%-58%.

2. The collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 1, characterized in that, The collagen peptides are produced by fermenting type II collagen using microbial fermentation.

3. The collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 2, characterized in that, The preparation of collagen peptides by the microbial fermentation method uses a combination of microbial fermentation agents, which include Bacillus, Enterobacter, and Aeromonas.

4. The collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 1, characterized in that, The dragon fruit fermented pulp is prepared by the following method: it is made from red dragon fruit fermentation liquid rich in short-chain fatty acids and galactooligosaccharides. The red dragon fruit fermentation liquid is obtained by a three-step directional fermentation method, which consists of aerobic and anaerobic fermentation, completely anaerobic fermentation, and completely aerobic fermentation.

5. A collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 4, characterized in that... The aerobic and anaerobic fermentation inoculation strain is yeast; the completely anaerobic fermentation inoculation strain is a microbial combination fermentation agent, which includes Bifidobacterium, Bacteroides, and Eubacterium; the completely aerobic fermentation inoculation strain is acetic acid bacteria.

6. The collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 5, characterized in that, The dragon fruit fermented pulp contains no fewer than 11 types of short-chain fatty acids and the content of short-chain fatty acids is ≥9%.

7. A collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 6, characterized in that, The short-chain fatty acids include acetic acid, propionic acid, and butyric acid, and the ratio of acetic acid, propionic acid, and butyric acid is 4:2:

1.

8. The collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 1, characterized in that, The medicinal and edible extract is one or more of the following: Solomon's seal extract, hawthorn extract, and Ganoderma lucidum extract.

9. A collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 1, characterized in that, The excipients are one or more of drinking water, sodium citrate, potassium sorbate, and sucralose.

10. A collagen peptide dragon fruit pulp fermented organic acid beverage with joint-repairing properties according to claim 1, characterized in that, The compound nutritional fortifiers are nicotinamide, vitamin B6, vitamin B12, and folic acid.

11. A method for preparing a fermented organic acid beverage of dragon fruit pulp containing collagen peptides that can repair joints, according to any one of claims 1 to 10, characterized in that, Includes the following steps: Weigh out collagen peptides, food and medicine homologous extracts, dragon fruit fermented pulp, acerola cherry extract, compound nutritional fortifiers, and excipients, mix and stir to dissolve, and defoam to obtain a collagen peptide dragon fruit pulp fermented organic acid beverage that can repair joints.

12. The method for preparing a fermented organic acid beverage containing collagen peptides from dragon fruit pulp that can repair joints according to claim 11, characterized in that, The defoaming process also includes bottling and sterilization.

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