Method for producing isopropanol

Abstract

The present invention relates to a method for producing isopropanol by using a recombinant microorganism derived from a hydrogen-oxidizing bacterium and also using carbon dioxide as a carbon source. The present invention provides a method for producing isopropanol from a carbon source, the method using a recombinant microorganism derived from a bacterium belonging to the genus Cupriavidus. The method comprises (1) a first step for culturing the recombinant microorganism derived from a bacterium belonging to the genus Cupriavidus until the expression level of an indicator biomolecule alters, and (2) a second step for producing isopropanol by culturing the recombinant microorganism derived from a bacterium belonging to the genus Cupriavidus, which is cultured in the first step, under conditions that the alteration of the indicator biomolecule is maintained, wherein the recombinant microorganism derived from a bacterium belonging to the genus Capryavidus has an acetoacetate decarboxylase gene and an alcohol dehydrogenase gene introduced thereto.

Description

Method for producing isopropanol

[0001] This patent application claims priority under the Paris Convention to and the benefit of Japanese Patent Application No. 2024-097677 (filed June 17, 2024) and Japanese Patent Application No. 2024-143267 (filed August 23, 2024), the entire contents of which are incorporated herein by reference. The present invention relates to a method for producing isopropanol from carbon dioxide using a recombinant microorganism derived from a hydrogen-oxidizing bacterium, particularly a bacterium of the genus Capriavidus. More specifically, the present invention relates to a method for producing isopropanol from carbon dioxide using a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus, comprising: (1) a first step of culturing a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until the expression level of an indicator biomolecule changes; and (2) a second step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus cultured in the first step while maintaining the change in the expression level of the indicator biomolecule to produce isopropanol, wherein the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus is a recombinant microorganism into which a gene for acetoacetate decarboxylase and a gene for alcohol dehydrogenase have been introduced.

[0002] Isopropanol (IPA) is useful as a biofuel.

[0003] A method for producing isopropanol from glucose as a carbon source using a recombinant microorganism derived from a hydrogen-oxidizing bacterium belonging to the genus Capriavidus has been known (Non-Patent Document 1).

[0004] It is known that in bioprocesses, in order to increase the productivity of a target substance, it is necessary to measure and control parameters such as intracellular information (Non-Patent Document 2).

[0005] Journal of Bioscience and Bioengineering, Volume 132, Issue 5, November 2021, pages 479-486, Journal of Chemical Engineering, Vol. 17 (1991), No. 3, pages 559-564

[0006] Capriavidus bacteria are useful microorganisms in bioproduction because they can use a variety of carbon sources as raw materials. ２ To reduce emissions, there is a need to establish a method for producing chemical raw materials using this bacterium, especially for the production of key chemicals such as isopropanol. To efficiently produce isopropanol, it is necessary to identify biomolecules that can serve as indicators for understanding the intracellular state.

[0007] As a result of intensive research in light of the above circumstances, the present inventors have discovered: a method for producing isopropanol from carbon dioxide using a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus, wherein the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus is a recombinant microorganism into which a gene for acetoacetate decarboxylase and a gene for alcohol dehydrogenase have been introduced; and a method for using one or more proteins or compounds selected from the following Group A as biomarkers in a method for producing isopropanol using a microorganism: Group A: one or more proteins selected from the proteins represented by SEQ ID NOs: 3 to 332; one or more compounds selected from glutamic acid, tyrosine, serine, leucine, and isoleucine; and have completed the present invention.

[0008] That is, the present invention may have the following configuration aspects, but is not limited to these: [1] A method for producing isopropanol from a carbon source using a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus, the method comprising: (1) a first step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until a change in the expression level of an indicator biomolecule occurs; and (2) a second step of culturing the recombinant microorganism derived from the bacterium belonging to the genus Capriavidus cultured in the first step while maintaining the change in the expression level of the indicator biomolecule to produce isopropanol, wherein the recombinant microorganism derived from the bacterium belonging to the genus Capriavidus is a recombinant microorganism into which a gene for acetoacetate decarboxylase and a gene for alcohol dehydrogenase have been introduced. [2] The production method according to [1], wherein the bacterium of the genus Cupriavidus is selected from the group consisting of Cupriavidus necator, Cupriavidus pinatubonensis, Cupriavidus laharis, and Cupriavidus metallidurans. [3] The production method according to [1], wherein the bacterium of the genus Cupriavidus is Cupriavidus necator. [4] The production method according to [1], wherein the acetoacetate decarboxylase gene is a or b, and the alcohol dehydrogenase gene is c or d. a: a gene encoding the amino acid sequence shown in SEQ ID NO: 1 b: a gene encoding an amino acid sequence having 90% or more identity to SEQ ID NO: 1 c: a gene encoding the amino acid sequence shown in SEQ ID NO: 2 d: a gene encoding an amino acid sequence having 90% or more identity to SEQ ID NO: 2 [5] The manufacturing method according to any one of [1] to [4], wherein the indicator biomolecule is a protein or a compound. [6] The manufacturing method according to any one of [1] to [5], wherein the fluctuation is an increase. [7] The manufacturing method according to any one of [1] to [5], wherein the fluctuation is a decrease.[8] The production method according to any one of [1] to [7], wherein the indicator biomolecule is one or more proteins or compounds selected from Group A. Group A: proteins and compounds including: one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 79 and 249 to 265; a protein comprising an amino acid sequence having 90% or more identity to one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 79 and 249 to 265; and one or more compounds selected from glutamic acid, tyrosine, serine, leucine, and isoleucine. [9] The production method according to [8], wherein the one or more proteins or compounds selected from Group A are: a protein set forth in SEQ ID NO: 3 to 26, 249, or 250; a protein comprising an amino acid sequence having 90% or more identity to one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 26, 249, or 250; and glutamic acid.

[10] The production method according to either [8] or [9], wherein the carbon source is one or more selected from the group consisting of sugars, carbon dioxide, glycerol, acetic acid, and formic acid.

[11] The production method according to either

[10] , wherein the carbon source is carbon dioxide.

[12] The production method according to either

[11] , wherein the reaction is carried out under hydrogen / carbon dioxide / oxygen conditions.

[13] The production method according to either

[12] , wherein the hydrogen concentration is 1 to 8% based on the total gas in the reaction system.

[14] The production method according to either

[12] or

[13] , wherein hydrogen is supplied by a proton exchange membrane (PEM) water electrolysis device using a manganese-iridium oxide composite (Mn-IrOx) as the anode.

[15] The production method according to any one of [1] to

[14] , wherein the yield of isopropanol per unit of electricity is 45% or more.

[16] The production method according to either [8] or [9], wherein the carbon source is glucose.

[17] An isopropanol production apparatus for use in the production method according to any one of

[11] to

[14] , comprising a PEM-type water electrolysis apparatus and a closed circulation-type culture apparatus.

[18] A method for using one or more proteins or compounds selected from the following Group A as biomarkers in a method for producing isopropanol using a microorganism: Group A: one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 79 and 249 to 265; a protein comprising an amino acid sequence having 90% or more identity to one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 79 and 249 to 265; and one or more compounds selected from glutamic acid, tyrosine, serine, leucine, and isoleucine.

[19] One or more proteins or compounds selected from the following Group A, which are used as biomarkers in a method for producing isopropanol using a microorganism: Group A: one or more proteins selected from the proteins shown in SEQ ID NOs: 3 to 26, 249, or 250; a protein comprising an amino acid sequence having 90% or more identity to one or more proteins selected from the proteins shown in SEQ ID NOs: 3 to 26, 249, or 250; and glutamic acid.

[0009] According to the present invention, there can be provided a method for producing isopropanol from a carbon source using a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus, the method comprising: (1) a first step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until a change in the expression level of an indicator biomolecule occurs; and (2) a second step of culturing the recombinant microorganism derived from the bacterium belonging to the genus Capriavidus cultured in the first step while maintaining the change in the expression level of the indicator biomolecule to produce isopropanol, wherein the recombinant microorganism derived from the bacterium belonging to the genus Capriavidus is a recombinant microorganism into which a gene for acetoacetate decarboxylase and a gene for alcohol dehydrogenase have been introduced. The present invention also provides a method for using one or more proteins or compounds selected from the following Group A as biomarkers in a method for producing isopropanol using a microorganism: Group A: one or more proteins selected from the proteins represented by SEQ ID NOs: 3 to 332; one or more compounds selected from glutamic acid, tyrosine, serine, leucine, and isoleucine.

[0010] 1 is a diagram showing a scheme of an isopropanol biosynthesis pathway; 2 is a diagram showing one embodiment of an isopropanol device; 3 is a diagram showing one embodiment of the principle of proton exchange membrane (PEM)-type water electrolysis; 4 is a diagram showing the gas composition in a culture tank and the amount of bacteria (OD ６００ ) and dissolved oxygen concentration (DO).

[0011] The following describes in detail the embodiments of the present invention. Note that the present invention is not limited to the following embodiments. Furthermore, the present invention also encompasses any combination of one or more of the embodiments described herein.

[0012] (Method for Producing Isopropanol) The method for producing isopropanol of the present invention (hereinafter referred to as the "production method of the present invention") relates to a method for producing isopropanol from a carbon source using a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus, wherein the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus is a recombinant microorganism into which a gene for acetoacetate decarboxylase and a gene for alcohol dehydrogenase have been introduced. More specifically, the method for producing isopropanol of the present invention (hereinafter referred to as "Production Method 1 of the Present Invention") is a method for producing isopropanol from a carbon source using a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus, which comprises: (1) a first step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until the expression level of an indicator biomolecule changes; and (2) a second step of culturing the recombinant microorganism derived from the bacterium belonging to the genus Capriavidus cultured in the first step while maintaining the change in the expression level of the indicator biomolecule to produce isopropanol, wherein the recombinant microorganism derived from the bacterium belonging to the genus Capriavidus is a recombinant microorganism into which a gene for acetoacetate decarboxylase and a gene for alcohol dehydrogenase have been introduced.

[0013] The biosynthesis pathway of isopropanol using bacterial enzymes is shown in the schematic diagram (Figure 1). Figure 1 is described in Journal of Bioscience and Bioengineering, Volume 132, Issue 5, November 2021, Pages 479-486. First, in step a) carbon dioxide (CO ２ step b) producing acetoacetyl-CoA from acetyl-CoA by the action of a recombinant microorganism of a bacterium of the genus Capriavidus; step b) producing acetoacetic acid from acetoacetyl-CoA by the action of a recombinant microorganism of a bacterium of the genus Capriavidus; step c) producing acetone from acetoacetic acid by the action of a recombinant microorganism of a bacterium of the genus Capriavidus; and step d) producing isopropanol from acetone by the action of a recombinant microorganism of a bacterium of the genus Capriavidus.

[0014] Examples of bacteria belonging to the genus Cupriavidus include, but are not limited to, Cupriavidus necator, Cupriavidus pinatubonensis, Cupriavidus laharis, and Cupriavidus metallidurans. Preferably, Cupriavidus necator is used.

[0015] A recombinant microorganism derived from a bacterium belonging to the genus Capriavidus (hereinafter also referred to as a "Capriavidus bacterium recombinant microorganism") is a recombinant bacterium into which a foreign DNA gene has been introduced into a bacterium belonging to Capriavidus. Examples of the introduced foreign DNA gene include, but are not limited to, the acetoacetate decarboxylase gene (i.e., the acetoacetate decarboxylase gene) that acts in the above-mentioned step c) and the alcohol dehydrogenase gene (i.e., the alcohol dehydrogenase gene) that acts in step d). The method for introducing the foreign DNA gene is not particularly limited as long as it is a method commonly known as a recombinant technique, and examples include methods using plasmids containing the acetoacetate decarboxylase gene and the alcohol dehydrogenase gene.

[0016] Examples of acetoacetate decarboxylases include, but are not limited to, enzymes containing an amino acid sequence with 90% or more identity to SEQ ID NO: 1. More preferably, the enzyme contains the amino acid sequence of SEQ ID NO: 1. Specific identities include, but are not limited to, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%. Examples of alcohol dehydrogenase genes include, but are not limited to, enzymes containing an amino acid sequence with 90% or more identity to SEQ ID NO: 2. More preferably, the enzyme contains the amino acid sequence of SEQ ID NO: 2. Specific identities include, but are not limited to, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

[0017] The gene encoding acetoacetate decarboxylase introduced into the isopropanol synthesis recombinant strain includes, but is not limited to, a gene having a nucleotide sequence with 80% or more identity to SEQ ID NO: 333. More preferably, the gene having the nucleotide sequence of SEQ ID NO: 333. Specific identities include, but are not limited to, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity. The gene encoding alcohol dehydrogenase introduced into the isopropanol synthesis recombinant strain includes, but is not limited to, a gene having a nucleotide sequence with 80% or more identity to SEQ ID NO: 334. More preferably, the gene having the nucleotide sequence of SEQ ID NO: 334. Specific identities include, but are not limited to, for example, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

[0018] Specific examples of recombinant microorganisms derived from bacteria belonging to the genus Cupriavidus include, but are not limited to, Cupriavidus necator IP-007 / pBj5c-adh-adc and IP-015 strains, which have genes having the nucleotide sequences of SEQ ID NOs: 333 and 334. Bacteria belonging to the genus Cupriavidus may also have a 16S rRNA gene containing a nucleotide sequence that is 90% or more identical to the nucleotide sequence of SEQ ID NO: 335. Specific identities include, but are not limited to, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%. Preferred specific identities include, for example, 95% or 99%. Examples include bacteria having a 16S rRNA gene containing the nucleotide sequences of SEQ ID NOs: 336 to 339.

[0019] Recombinant microorganisms derived from bacteria belonging to the genus Capriavidus also include microorganisms containing at least one protein or compound selected from Group A below. Group A: at least one protein selected from the proteins set forth in SEQ ID NOS: 3 to 332; and at least one compound selected from glutamic acid, tyrosine, serine, leucine, and isoleucine. The at least one protein or compound selected from Group A preferably includes the proteins set forth in SEQ ID NOS: 3 to 79 and 249 to 265, and glutamic acid. The at least one protein or compound selected from Group A preferably includes the proteins set forth in SEQ ID NOS: 3 to 26 and 249 or 250, and glutamic acid.

[0020] The proteins or compounds included in Group A are indicator biomolecules (markers) that can serve as indicators of the degree of reaction activity in the isopropanol production method of the present invention. By monitoring changes in the expression level of these indicator biomolecules, the performance of the recombinant Capriavidus microorganism, which is the biocatalyst used in the production method, can be evaluated. Proteins are measured and evaluated by proteome analysis, and compounds are measured and evaluated by metabolomic analysis, between the start and end of the isopropanol production reaction. Proteins or compounds that show a large fold change can be considered indicator biomolecules (markers) that contribute significantly to the isopropanol production reaction. The microorganisms used as biocatalysts in the production reaction of the present invention contain one or more of these indicator biomolecules that show a large fold change (e.g., 2 or more, 3 or more, 4 or more, 5 or more, 10 or more, 15 or more, 20 or more, 30 or more, 40 or more, 50 or more, 60 or more, 70 or more, 80 or more, 90 or more, or 100 or more). The magnitude of the fold change, when the variation increases, can include, for example, 2x or more, 3x or more, 4x or more, 5x or more, 6x or more, 7x or more, or 8x or more; or, when the variation decreases, can include, for example, 1 / 2 or less (e.g., 1 / 3 or less, 1 / 4 or less, 1 / 5 or less, 1 / 6 or less, 1 / 7 or less, 1 / 8 or less).

[0021] Methods for measuring proteins include, for example, LC-MS, ELISA, and Western blot.

[0022] The fold change can be calculated, for example, by dividing the quantitative value in the IPA production period by the quantitative value in the growth period. The IPA production period may be divided according to time. For example, the IPA production period may be divided into an early IPA production period and a late IPA production period, and the fold change may be calculated by dividing the quantitative value in the early IPA production period by the quantitative value in the growth period.

[0023] Proteome analysis and metabolome analysis are calculated not only from the results of a production method using carbon dioxide as a carbon source, but also from the results of a production method using glucose as a carbon source. By performing comprehensive omics analysis based on the results of both production methods, indicator biomolecules can be identified with greater accuracy.

[0024] In the proteome analysis, proteins with large or small fold changes include proteins listed in any one of Tables 1 to 6 below. Here, proteins that showed very large fold changes were proteins listed in Table 1, proteins that showed very large fold changes were proteins listed in Table 2, and proteins that showed large fold changes were proteins listed in Table 3. Proteins that showed very small fold changes were proteins listed in Table 4, proteins that showed very small fold changes were proteins listed in Table 5, and proteins that showed small fold changes were proteins listed in Table 6.

[0025] Table 1

[0026] Table 2

[0027] Table 2 (continued)

[0028] Table 3

[0029] Table 3 (continued)

[0030] Table 3 (continued)

[0031] Table 3 (continued)

[0032] Table 3 (continued)

[0033] Table 4

[0034] Table 5

[0035] Table 6

[0036] Table 6 (continued)

[0037] Table 6 (continued)

[0038] In metabolomic analysis, compounds with small fold changes include those listed in Table 7 below. Here, the compound that showed a very small fold change was glutamic acid listed in Table 7, and the compounds that showed small fold changes were tyrosine, serine, leucine, and isoleucine listed in Table 7. Table 7

[0039] Therefore, proteins included in the above Group A include proteins and compounds consisting of one or more of the proteins listed in any one of Tables 1 to 6 and one or more of the compounds listed in Table 7, preferably proteins and compounds consisting of one or more of the proteins listed in any one of Tables 1 to 2 and Tables 4 to 5 and one or more of glutamic acid, tyrosine, serine, leucine, and isoleucine listed in Table 7, and more preferably proteins and compounds consisting of one or more of the proteins listed in Tables 1 and 4 and glutamic acid listed in Table 7.

[0040] The production method of the present invention may include: (1) a first step of culturing a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until the expression level of an indicator biomolecule changes; and (2) a second step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus cultured in the first step while maintaining the change in the expression level of the indicator biomolecule, thereby producing isopropanol.

[0041] In the first step, the catalytic function of the recombinant microorganism of the genus Capriavidus used as a biocatalyst can be fully exerted by measuring and confirming the expression level of the indicator biomolecule until it fluctuates to a certain extent. For example, under the reaction conditions of the production method of the present invention, the first step can be the incubation time from the start of the reaction until a certain time has passed. For example, the first step can be the incubation time from the start of the reaction until 50 hours later, but this incubation time can vary depending on the reaction conditions.

[0042] In the second step, isopropanol can be produced efficiently by continuing the culture after the first step while maintaining a certain level of the fluctuating expression level of the indicator biomolecule. When the expression level of the indicator biomolecule is increased in the first step, the certain level of expression level to be maintained can be, but is not limited to, 80% or more, 85% or more, 90% or more, 95% or more, or 100% compared to the level at the end of the first step. Preferably, it can be 90% or more, and more preferably 95% or more. When the expression level of the indicator biomolecule is decreased in the first step, the certain level of expression level to be maintained can be, but is not limited to, 120% or less, 115% or less, 110% or less, 105% or less, or 100% compared to the level at the end of the first step. Preferably, it can be 110% or less, and more preferably 105% or less. The culture time in the second step can be up to 50 hours, 60 hours, 70 hours, 80 hours, 90 hours, 100 hours, 110 hours, 120 hours, 130 hours, 140 hours, 150 hours, 200 hours, or more than 200 hours (e.g., more than 250 hours, and more than 300 hours) from the start of the reaction, but the culture time can vary depending on the reaction conditions. For example, the culture time can be from 90 to 100 hours from the start of the reaction.

[0043] The production method of the present invention can be carried out under mixed gas conditions of hydrogen / carbon dioxide / oxygen.

[0044] In the production method of the present invention, carbon dioxide gas can be used as a carbon source, and since it is a method of autotrophic culture, it is possible to avoid the mixing of raw material impurities, intermediate impurities, and particularly solid impurities in the reaction system, compared to when sugar is used as a carbon source.

[0045] The concentration of hydrogen gas is, for example, 0.5 to 90%, preferably 1 to 8%, and more preferably 1 to 4%, but is not limited to these. The concentration of hydrogen used in the production method of the present invention is low compared to the 60% hydrogen gas concentration used in the reaction conditions of a previously reported method for producing isopropanol under hydrogen / oxygen gas conditions using carbon dioxide as a carbon source. The ability to carry out the reaction at such a low concentration of hydrogen is useful because it can significantly reduce the risk of hydrogen explosion at a production scale, particularly at an industrial production level.

[0046] The concentration of carbon dioxide gas is, for example, 1 to 30%, and preferably 3 to 15%, but is not limited to these. The concentration of oxygen gas is, for example, 0.01 to 20%, and preferably 0.1 to 15%, but is not limited to these.

[0047] The ratio of the concentrations of hydrogen / carbon dioxide / oxygen gases is, for example, 1-10:1-100:1-100, and may be, for example, 3:10:10, but is not limited to this.

[0048] In addition to hydrogen, carbon dioxide, and oxygen, any gas component may be added during the culture. Examples of such gas components include nitrogen and argon. Nitrogen is preferred.

[0049] In the production method of the present invention, when carbon dioxide is used instead of sugar (e.g., glucose) as a carbon source, a nitrogen source-limited inorganic salt medium containing no carbon source can be used as the medium. Nitrogen is used for growing the bacterial recombinant microorganism that functions as a biocatalyst in the production method of the present invention. Ammonium chloride (NH ４Examples of the additives include, but are not limited to, disodium hydrogen phosphate hydrate (e.g., disodium hydrogen phosphate dodecahydrate), potassium dihydrogen phosphate, magnesium sulfate hydrate (e.g., magnesium sulfate heptahydrate), and trace metal solutions (trace metals include iron (Fe), cobalt (Co), copper (Cu), nickel (Ni), and chromium (Cr)), and kanamycin sulfate.

[0050] In one embodiment, the production method of the present invention may include: (1) a first step of culturing a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until the expression level of an indicator biomolecule increases; and (2) a second step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus cultured in the first step while maintaining the increase in the expression level of the indicator biomolecule, thereby producing isopropanol.

[0051] In the first step (1) of culturing a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until the expression level of a marker biomolecule increases, for example, when a nitrogen source medium is used, the recombinant microorganism is cultured in a nitrogen source (e.g., NH ４ The first step can be the period when the growth of the recombinant microorganism stops due to nitrogen source depletion caused by exhaustion of nitrogen (Cl). The second step can be the period from the subsequent isopropanol (IPA) biosynthesis stage.

[0052] By measuring and confirming the expression level of the indicator biomolecule until it reaches a certain level, the catalytic function of the recombinant Capriavidus bacterium used as a biocatalyst can be fully exerted. For example, under the reaction conditions of the production method of the present invention, the first step can be the incubation time from the start of the reaction until a certain time has elapsed. For example, the first step can be the incubation time from the start of the reaction until 50 hours later, but this incubation time can vary depending on the reaction conditions.

[0053] In the second step, isopropanol can be produced efficiently by continuing the culture after the first step while maintaining the increased expression level of the indicator biomolecule at a certain level.

[0054] In another embodiment, the production method of the present invention may include: (1) a first step of culturing a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until the expression level of an indicator biomolecule is reduced; and (2) a second step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus cultured in the first step while maintaining the reduced expression level of the indicator biomolecule to produce isopropanol.

[0055] In the first step (1) of culturing a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until the expression level of an indicator biomolecule decreases, for example, when a nitrogen source medium is used, the recombinant microorganism ４ The first step can be the period when the growth of the recombinant microorganism stops due to nitrogen source depletion caused by exhaustion of nitrogen (Cl). The second step can be the period from the subsequent isopropanol (IPA) biosynthesis stage.

[0056] By measuring and confirming the decrease in the expression level of the indicator biomolecule to a certain extent, the catalytic function of the recombinant Capriavidus bacterium used as a biocatalyst can be fully exerted. For example, under the reaction conditions of the production method of the present invention, the first step can be the incubation period from the start of the reaction until a certain time has elapsed. For example, the first step can be the incubation period from the start of the reaction until 50 hours later, but this incubation period can vary depending on the reaction conditions.

[0057] In the second step, isopropanol can be produced efficiently by continuing the culture after the first step while maintaining a certain level of the decreased expression level of the indicator biomolecule.

[0058] In the first step (1) of culturing a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until the expression level of a marker biomolecule increases, for example, when a nitrogen source medium is used, the recombinant microorganism is cultured in a nitrogen source (e.g., NH ４The first step can be the period when the growth of the recombinant microorganism stops due to nitrogen source depletion caused by exhaustion of nitrogen (Cl). The second step can be the period from the subsequent isopropanol (IPA) biosynthesis stage.

[0059] By measuring and confirming the expression level of the indicator biomolecule until it reaches a certain level, the catalytic function of the recombinant Capriavidus bacterium used as a biocatalyst can be fully exerted. For example, under the reaction conditions of the production method of the present invention, the first step can be the incubation time from the start of the reaction until a certain time has elapsed. For example, the first step can be the incubation time from the start of the reaction until 50 hours later, but this incubation time can vary depending on the reaction conditions.

[0060] In the second step, isopropanol can be produced efficiently by continuing the culture after the first step while maintaining the increased expression level of the indicator biomolecule at a certain level.

[0061] (Production Apparatus) The production method of the present invention can be carried out using an apparatus (FIG. 2) having a PEM water electrolysis apparatus and a closed circulation-type culture apparatus. The PEM water electrolysis apparatus is an apparatus that generates hydrogen and supplies the hydrogen to a closed circulation-type culture apparatus. The apparatus contains a PEM reactor with a manganese-iridium oxide composite (Mn-IrOx) (WO 2021 / 193467) on the anode electrode side and a Pt-Ti mesh on the cathode electrode side.

[0062] The closed circulation type fermentation equipment is a device that produces isopropanol by culturing biocatalytic microorganisms under hydrogen / carbon dioxide / oxygen gas conditions. The gas concentration in the circulation gas phase is kept constant by feedback control based on the concentration measured online.

[0063] Isopropanol yield The isopropanol yield is calculated as follows. First, since the circulating gas concentration is kept almost constant during the cultivation period, it can be seen that the amount of gas consumed by the bacteria is in balance with the amount of gas supplied. The following stoichiometric formula is obtained from the ratio of the amounts of each gas substrate introduced into the circulating gas phase during the bacterial growth phase (12 to 36 hours) and the isopropanol biosynthesis phase (72 to 96 hours). Cell growth (12 to 36 hours) Isopropanol biosynthesis period (72-96 hours) From the amount of bacterial cells and isopropanol biosynthesized during this period, as well as the amount of electricity and the electrolysis rate (70%), the H of bacterial growth and isopropanol biosynthesis was calculated. ２ The yield per unit of power and the yield per unit of electricity are calculated. The yield of isopropanol per unit of power is preferably 30% or more, more preferably 35% or more, even more preferably 40% or more, and particularly preferably 45% or more.

[0064] Furthermore, assuming that the above-mentioned electricity is obtained from solar panels and that the energy conversion efficiency of silicon solar cells is 20%, the highest level currently available, the isopropanol (IPA) yield per solar energy (equivalent solar-to-PHA) in the present invention is a high 9.5%. Therefore, it can be said that efficient mass exchange from electrical energy is possible in the culture according to the present invention.

[0065] The method for producing isopropanol of the present invention can also be carried out using glucose instead of carbon dioxide as a carbon source when a microorganism or recombinant microorganism containing a protein or compound belonging to Group A that can serve as the aforementioned indicator biomolecule (marker), other than Cupriavidus necator IP-007 / pBj5c-adh-adc strain, is used.

[0066] The present invention will be explained in more detail below with reference to examples, but the present invention is not limited to these examples.

[0067] Example 1: Bacterial Strain Used The Cupriavidus necator IP-007 / pBj5c2-adh-adc strain, developed by the present inventors, is a modified strain capable of biosynthesizing isopropanol (IPA) from glucose (Figure 1). The IP-007 strain was created by conferring glucose assimilation ability to a wild-type C. necator strain (J. Biosci. Bioeng., 113:63-69, 2012), and by deleting the polyhydroxyalkanoate (PHA) synthase gene phaC1, the NADPH-acetoacetyl-CoA reductase genes phaB1 and phaB3, and the NADH-3-hydroxyacyl-CoA dehydrogenase genes paaH1 and had. The IP-007 / pBj5c2-adh-adc strain was constructed by introducing the plasmid pBj5c2-adh-adc, which contains the genes for acetoacetate decarboxylase and alcohol dehydrogenase, which convert acetoacetate to isopropanol, into the IP-007 strain. This strain has been shown to biosynthesize isopropanol from glucose (J. Biosci. Bioeng. 132:479-486, 2021). In this study, this recombinant strain was subjected to hydrogen and glucose cultivation under hydrogen / carbon dioxide / oxygen conditions.

[0068] Example 2 Gas-Phase Circulation Cultivation and Supply of Low-Concentration Hydrogen by Water Electrolysis C. necator IP-007 / pBj5c2-adh-adc strain was cultured in a 2 L Jar Fermenter (manufactured by Biot) in a nitrogen-source-limited inorganic salts medium A-1 (culture volume: 1 L) containing no carbon source (0.9% disodium hydrogen phosphate dodecahydrate, 0.15% potassium dihydrogen phosphate, 0.05% ammonium chloride, 0.02% magnesium sulfate heptahydrate, 1% trace metal solution (9.7 g / L FeCl3, 7.8 g / L CaCl2, 0.218 g / L CoCl2.6H2O, 0.156 g / L CuSO4.5H2O, 0.118 g / L NiCl3.6H2O, 0.105 g / L CrCl3.6H2O, 0.1 M HCl), and 100 mg / L kanamycin sulfate. The culture solution was supplied with a mixture of carbon dioxide supplied by a mass flow controller (Fcon C2000) and hydrogen and oxygen generated from a proton exchange PEM reactor (FC-R&D). The mixture was blown into the culture solution at 600 mL / min using a diaphragm pump, and piping was installed to circulate the exhaust gas (Fig. 2). Hydrogen was generated under constant current conditions of approximately 1.5 to 2 V applied from a power supply to a PEM reactor (Fig. 3) equipped with a membrane catalyst consisting of a Pt-Ti mesh as the cathode and a Mn-IrOx membrane catalyst as the anode, and introduced into the circulation flow path. Multilayer plastic tubing with a gas barrier layer was used for the piping, and O was introduced into a bypass flow path from the gas phase circulation flow path. ２ / CO ２ Detector (manufactured by Biot) and H ２ A detector (manufactured by Biot) was installed to measure the gas composition online.

[0069] The carbon dioxide supply flow rate and water electrolysis current value were controlled by a mass flow controller using feedback control based on online measurements to maintain constant carbon dioxide and hydrogen concentrations in the gas phase. The excess oxygen generated by water electrolysis was maintained at a constant oxygen concentration using a three-way solenoid valve (manufactured by CKD Corporation), the opening and closing of which was feedback-controlled based on online measurements. The culture temperature was set to 30°C, and stirring was performed at 400 rpm / min using a stirring blade. A gas bag was installed to adjust the pressure of the circulating gas phase. The circulating gas had a composition of approximately hydrogen:oxygen:carbon dioxide:nitrogen = 4:7:12:77.

[0070] Example 3 Analysis Turbidity at 600 nm was measured as an index of bacterial cell concentration. The supernatant obtained by removing bacterial cells from the culture solution by centrifugation was filtered through a cellulose acetate filter (pore size: 2 micrometers) and subjected to high performance liquid chromatography to measure the isopropanol (IPA) concentration. For high performance liquid chromatography analysis, an Aminex-HPH-87H column (300 mm x φ7.9 mm) (manufactured by Bio-Rad) was used, and the eluent was 2.5 mM sulfuric acid (H ２ SO ４ ) in water and detected using a differential refractometer RID-10A (Shimadzu Corporation).

[0071] Results: With the supply of low-concentration hydrogen from the PEM reactor, the bacteria grew well using carbon dioxide as a carbon source, and the final bacterial concentration was measured by the turbidity at a wavelength of 600 nm (OD ６００ ) reached 4.2 (Fig. 4). ４ When the nitrogen source was depleted, bacterial growth stopped and the culture entered the isopropanol (IPA) biosynthesis phase. The isopropanol (IPA) concentration in the culture supernatant is shown in Table 8. After bacterial growth stopped, the isopropanol (IPA) concentration increased and reached 1.60 g / L after 144 hours.

[0072] Table 8 It was found that isopropanol can be biosynthesized under reaction conditions using low concentrations of hydrogen and carbon dioxide.

[0073] (Reference Example 1) Culture using glucose as a carbon source The C necator IP-007 / pBj5c2-adh-adc strain was cultured (culture volume 100 mL) in nitrogen-source-limited inorganic salt medium A-1 to which glucose was added to a final concentration of 20 g / L. The culture was performed using a flask with shaking at 30°C. The cells grew well using glucose as a carbon source, and the final cell concentration was measured by the turbidity at 600 nm (OD ６００ ) reached 4 (Table 9). At 48 hours of culture, the nitrogen source (NH ４ When the nitrogen source was depleted, bacterial growth stopped and the culture entered the isopropanol (IPA) biosynthesis phase. The isopropanol (IPA) concentration in the culture supernatant is shown in Table 9. After bacterial growth stopped, the isopropanol (IPA) concentration increased and reached 3.13 to 3.32 g / L after 120 hours.

[0074] Table 9

[0075] Proteome Analysis (Proteome Sample Preparation) Methanol was added to the bacterial cells and vacuum-lyophilized using a vacuum cooler (EYELA4) and a centrifuge (LABCONCO). The dried bacterial cells were suspended in 8 M urea and 50 mM ammonium bicarbonate solution and centrifuged. The supernatant was collected and used as the extract for analysis. The protein concentration of the extract was measured using a BCA protein assay kit (Thermo Fisher). The measurement instrument was a SpectraMax i3 (Molecular Devices), and absorbance at 562 nm was used as an index. The extract was diluted with urea and ammonium bicarbonate solution to achieve equal protein concentrations. Proteins in the extract were reductively alkylated with dithiothreitol (DTT) and iodoacetamide (IAA). After adding 50 mM ammonium bicarbonate to reduce the urea concentration to below 1 M, the proteins were digested with lysyl endopeptidase (Lys-C) and trypsin. The digested sample was acidified with trifluoroacetic acid. To correct for measurement errors between samples, an equal amount of iRT peptides (Biognosys) was mixed with each sample. Samples were desalted using a GL-Tip SDB (GL Sciences). Proteins were dissolved in 80% acetonitrile and 0.1% trifluoroacetic acid solution and then vacuum-lyophilized using a vacuum cooling device (EYELA4) and a centrifuge (LABCONCO). A 0.1% formic acid solution was added to the lyophilized sample, and the sample was used for nanoLC-MS analysis.

[0076] (Nano LC-MS analysis) Nano LC-MS analysis was performed using a nanocolumn (Acclaim PepMap 100 C18 75 μm (ID) x 50 cm, 2 μm (Thermo Fisher Scientific)) and a mass spectrometer (Q Exactive HF (Thermo Fisher Scientific)), and a method for Data-Independent Acquisition (DIA) was created to acquire data.

[0077] (Protein Identification) A search database (search DB) was created based on the following protein sequences. The data obtained by nanoLC-MS analysis was analyzed using the proteome analysis software DIA-NN 1.8.1 to identify and quantify proteins: C. necator Reference Proteome (UniProt Proteome ID: UP000008210), alcohol dehydrogenase (UniProt ID: P25984|ADH_CLOBE), acetoacetate decarboxylase (UniProt ID: P23670|ADC_CLOAB), and iRT-Kit_WR_fusion peptide (Biognosys). The quantitative values ​​for each protein were corrected using the internal standard peptide iRT quantitative values ​​for each sample. For glucose cultures, the quantitative values ​​from two flasks were averaged, and the data obtained from two nanoLC-MS analyses were then averaged and used to calculate fold changes.

[0078] Metabolomic analysis (metabolomic analysis using gas chromatography-mass spectrometry (GC-MS)) Bacterial cells were suspended in 50% methanol and disrupted using a sonicator (TOMY Handy Sonic UR-21P). The disrupted cells were centrifuged and the supernatant was collected. Aliquots of the supernatant were freeze-dried using a vacuum cooling device (EYELA4) and a centrifuge (LABCONCO). 2-Isopropylmalic acid solution (solvent: methanol) was added to the freeze-dried bacterial extract and the mixture was again freeze-dried. Methoxyamine solution (solvent: pyridine) was added to suspend the extract. The mixture was heated and stirred at 30°C for 90 minutes at 1000 rpm. N-Methyl-N-trimethylsilyl Trifluoroacetamide was added and suspended. The mixture was heated and stirred at 40°C for 30 minutes at 1000 rpm. After centrifugation, the supernatant was used as a sample for GC-MS analysis. The GC-MS analysis was performed using a GC capillary column (DB-5 (length 30 m, inner diameter 0.25 mm) (Agilent)) and a mass spectrometer (GCMS-TQ8040 (Shimadzu Corporation)), and data were acquired using a Multiple Reaction Monitoring (MRM) method created in advance for each target component. The measured peak area was calculated as OD600 The quantitative value was corrected by .

[0079] Calculation of Fold Changes. Hereinafter, the growth phase, early IPA production phase, and late IPA production phase were defined as 24, 72, and 120 hours, respectively, for gas culture, and 24, 48, and 120 hours, respectively, for glucose culture. The quantified value for the early IPA production phase divided by the quantified value for the growth phase was defined as fold change A. The quantified value for the late IPA production phase divided by the quantified value for the growth phase was defined as fold change B. Under both glucose and hydrogen culture conditions, biomolecules with both fold change A and fold change B greater than 1 were defined as upwardly fluctuating molecules, while biomolecules with both fold change A and fold change B less than 1 were defined as downwardly fluctuating molecules. For upwardly fluctuating molecules, the smaller of fold change A or fold change B was defined as the representative fold change. For downwardly fluctuating molecules, the larger of fold change A or fold change B was defined as the representative fold change.

[0080] Metabolomic analysis: For the downwardly varying molecules of the compounds, the fluctuating compounds that showed an extremely small representative fold change of less than one-quarter (◎), and the fluctuating compounds that showed an extremely small fold change of less than one-half and more than one-quarter (◯) are shown in Table 10 below.

[0081] Table 10

[0082] Proteome Analysis For the protein molecules with increasing fluctuations, the proteins with a representative fold change of 8-fold or more (◎), those with a very large fold change of 4-fold or more but less than 8-fold (◯), and those with a large fold change of 2-fold or more but less than 4-fold (△) are shown in Table 11 below. For the protein molecules with decreasing fluctuations, the proteins with a representative fold change of 1 / 8 or less (◎), those with a very small fold change of 1 / 4 or less but more than 1 / 8 (◯), and those with a small fold change of 1 / 2 or less but more than 1 / 4 (△) are shown in Table 12 below.

[0083] Table 11

[0084] Table 11 (continued)

[0085] Table 11 (continued)

[0086] Table 11 (continued)

[0087] Table 11 (continued)

[0088] Table 11 (continued)

[0089] Table 11 (continued)

[0090] Table 11 (continued)

[0091] Table 12

[0092] Table 12 (continued)

[0093] Table 12 (continued)

[0094] Isopropanol yield The isopropanol yield was calculated as follows. First, since the circulating gas concentration was kept almost constant during the cultivation period, it was found that the amount of gas consumed by the cells was in balance with the amount of gas supplied. The following stoichiometric formula was obtained from the ratio of the amounts of each gas substrate introduced into the circulating gas phase during the cell growth phase (12 to 36 hours) and the isopropanol biosynthesis phase (72 to 96 hours). Cell growth (12 to 36 hours) Isopropanol biosynthesis period (72-96 hours) From the amount of bacterial cells and isopropanol biosynthesized during this period, as well as the amount of electricity and the electrolysis rate (70%), the H of bacterial growth and isopropanol biosynthesis was calculated. ２ The yield per unit of production and the yield per unit of electricity were calculated. The results of the bacterial cell growth and the yield of IPA biosynthesis in Cupriavidus necator IP-007 / pBj5c-adh-adc strain are shown in Table 13 below.

[0095] Table 13

[0096] The sequences of the present invention are shown below (amino acid sequences are represented by single letter codes): SEQ ID NO: 1 is the amino acid sequence of acetoacetate decarboxylase, SEQ ID NO: 2 is the amino acid sequence of alcohol dehydrogenase, and SEQ ID NOs: 3 to 332 are the amino acid sequences of proteins in Group A from which indicator biomolecules are selected. SEQ ID NO: 1 Sequence length: 351, Organism: Clostridium beijerinckii, PRT MKGFAMLGINKLGWIEKERPVAGSYDAIVRPLAVSPCTSDIHTVFEGALGDRKNMILGHEAVGEVVEVGSEVKDFKPGDRVIVPCTTPDWRSLEVQAGFQQHSNGMLAGWKFSNFKDGVFGEYFHVNDADMNLAILPKDMPLENAVMITDMMTTGFHGAELADIQMGSSVVVIGIGAVGLMGIAGAKLRGAGRIIGVGSRPICVEAAKFYGATDILNYKNGHIVDQVMKLTNGKGVDRVIMAGGGSETLSQAVSMVKPGGIISNINYHGSGDALLIPRVEWGCGMAHKTIKGGLCPGGRLRAEMLRDMVVYNRVDLSKLVTHVYHGFDHIEEALLLMKDKPKDLIKAVVIL SEQ ID NO: 2 Sequence length: 244, Organism: Clostridium acetobutylicum strain ATCC824, PRT MLKDEVIKQISTPLTSPAFPRGPYKFHNREYFNIVYRTDMDALRKVVPEPLEIDEPLVRFEIMAMHDTSGLGCYTESGQAIPVSFNGVKGDYLHMMYLDNEPAIAVGRELSAYPKKLGYPKLFVDSDTLVGTLDYGKLRVATATMGYKHKALDANEAKDQICRPNYMLKIIPNYDGSPRICELINAKITDVTVHEAWTGPTRLQLFDHAMAPLNDLPVKEIVSSSHILADIILPRAEVIYDYLK SEQ ID NO: 3 Sequence length: 515, Organism: Cupriavidus necator H16, PRTMTLPGLALFYGGLVRSKNMLSVLMQCLVIFSLVALLWAIYGYSFAFTEGNAFFGGTDRLFMKGLTVEAVAATFSKGVVVPELGYFAFQCAFACITCGLIIGAFAERAKFSAVLVFVVLWFTFSYIPMAHMVWFWPGPDAYTDAAAGAAATAKSGWLFQKGALDFAGTVVHINAAVAGLVGAYMFGKRIGFGREAIRPHSLTFTMVGASLLWFGWFGFNAGSALEANGSAALAFVNTLLATCAAVLAWTFGEWIGKGKPSMLGGASGAVAGLVAITPAAGFVGPMGSIVMGLIAGLLCLWGVTGLKRMLGMDDSLVFGVHGVGGIVGALLTGVFAAPSLGGTGIYDYVANKVADDYSIAGQLWIQFEGVLTTVVWSGVVAFVAYKLVDMLIGLRVPEDEEREGLDITSHGETAYEV Sequence number 4 Sequence length: 179, Organism: Cupriavidus necator H16, PRT MTDKPFLSDVKTLRERARKHIDDGAVTAGYAADRDTVLKLLNDALATEIVCVLRYRRHYYMAKGPNSKSVADEFLAHSNEEQGHADQIAERIVQLGGEPDFAPDGLTSRSHAEYVAGTSLTEMIKEDLVAERIAIDSYREIIQFLGERDPTSRRMMEGILAVEEEHADEMADLLARQGG Sequence number 5 Sequence length: 361, Organism: Cupriavidus necator H16, PRTMSTVARFEIGYTRYLAPPGDTSSPTSPPPPLASNPDALLPLYQAMVLTRQFDLKAIALQRTGKIGTFASALGQEAIGVGVAFAMRPEDVLVPSYRDHAAQFVRGVTMAESLLYWGGDERGSGFAAAPHDFANCVPIGTQVCHAAGAAYAFQLRGEPRVAVCLLGDGGTSKGDFYEGMNMAGAWHAPLVIVINNNQWAISMPRSGQTAAQTLAQKAIAAGIPGEQVDGNDVVAVRHRVGEAIARARDGGGPALIEAITYRLGDHTTADDASRYRDEASVKAHWQEEPLLRLRTHLLALHAWDAAREEALVKACSQQVAQAVETYLAVPQPDPAAMFDCLYATMPAELQAQLEIARRFLAPHG SEQ ID NO: 6, Sequence Length: 112, Organism: Cupriavidus necator H16, PRT MKLIIAVIKPFKLDEVREALSDVGVSGITVTEVKGFGRQKGHTELYRGAEYIVDFLPKVKIEVAVPDDVVERAIEAVEKSARTGKIGDGKIFVAPIEQVIRIRTGETGGDAL SEQ ID NO: 7, Sequence Length: 436, Organism: Cupriavidus necator H16, PRTMPSRLRIAKPLHAAVHVPDAAADAVPASSGRRRALATIAGASVMTLVDPLVRAGAWAAGSDAPEKTDVRIGFIPLTDCASVVMAATLGLDKKYGIKITPTKEASWAGVRDKLVNGELDAAHVLYGLIYGVQLGIGGPKKDMAVLMNLNHNGQAITLSSKLAEKGVKDGASLKALMMKEKRDYTFAQTFPTGTHAMWLYYWLAANGINPMQDAKAITVPPPQMVANMRVGNMDGFCVGEPWGARAIADKIGFTAETTQAIWKGHPEKTLGTTAEFVQKYPNTARAMVAAVLEASKFIDASASNRRKTAETIAAKSYVNTDMDIILDRMLGRYTNGLGKTWDDPDAMKFYQDGAANYPYLSDGMWFLTQHKRWGLLKEHPDYLAVARQVNRIDVYKQAAAAAQVPLPKSDMRSAKLIDGVVWDGKDPKAYADGFKIKA Sequence number 8 Sequence length: 192, Organism: Cupriavidus necator H16, PRT MLRILLVRDPHDADPLNVETIRGGLVSAGFTEIQIVDADLRLPDVITATQPDMLIIASESAARDTIEHVCVSTQHAPRPIVLFTDNDDSQRIKAALTAGITAYIVDGLRAERVKTVLDVAYTRFELDQQLRAELDARLKLAERKVVERAKGLLMQARGLSEDEAYKRLRSMAMERGLKLVDAAQRVIDVMG Sequence number 9 Sequence length: 155, Organism: Cupriavidus necator H16, PRT MDKSKIPADQAQALGALMDDHRAVKKQFKAFKDTEDRDEKQAIALDVCHQLTVHATIEEEIFYPALRGVSDEIDDLDEAEVEHQVAKDLIAAIEEDPAGDKLEANYTVLSEYVAHHIEEEEGELFKNAIKEKVNLRDVAQAMAAARCEELMRETA Sequence number 10 Sequence length: 418, Organism: Cupriavidus necator H16, PRTMQRRDMLKLSAIAAAMIGTSPLAMAQSKEPIKVGILHSLSGTMAISETSLKDVALMTIDEINKSGGVLGRKLEPVVDPASNWPLFAEKARQLITKDKVAVTFGCWTSVSRKSVLPVYEELNSLLFYPQYEGEEMSKNVFYTGAAPNQQAIPAVEYLMSKEGGGAKRFFLLGTDYVYPRTTNKILRAFLKSKGVADKDIEEVYTPFGHSDYQTIVANIKKFSQGGKTAVISTINGDSNVPFYKELGNAGLKAKDVPVVAFSVGEEELRGIDTKPLVGHLAAWNYFMSVKNPENDAFRKQWAAWVKANNLPGGDKRVTNDPMEATYVGIHMWAQAVKKAGTTDTNKVRAAMYGQTFKAPDGFTLTMGENHHLYKPVMIGEVKGDQFSVVWKTPKAVRAQPWSPFIAGNEGKPDKVM

[0097] Sequence number 11, Sequence length: 558, Organism: Cupriavidus necator H16, PRT MHHDPSLLAKGLKSAASVMSAAVLSLLALPGAAHADKVLRIGMTAADIPRTLGQPDQGFEGNRFSGIPMYDGLTQWDLSRGNGPSLLIPGLATEWKVDAADKTRWVFKLRPGVKFHDGTPFNADAVVWNVGKVLDKTARQFDPSQVGVTASRMPTLRTAKKIDDFTVELVTSEPDSFLPYNLTNLFMASPTQWEKKFAAVPASVTDPVERSKQAWVAFAADAAGTGPFRMSRFVPRERLEVVRNAQYWDTKRVPKIDKVVMVPMPEANARTAALLSGQVDWIEAPAPDAIAQIKSRGFQVYANAQPHMWPWQLSFAPGSPWLDKRVRQAANLCVNRSGLKTLLGGYMAEAKGIVEAGNPWWGNPKFDIKYDPAAAKKLMQEAGYSAAKPVKVKVQVSASGSGQMQPLPMNEYVQQNLKECFIDVDFDVVEWNTLFTNWRVGAKDASAHGANAINVSFAAMDPFFAMVRFVSTKTQPPVSNNWGYFGNAEFDKLIENARTSFDEKSRDAALARLHARIVEESPFVLIAHDVGPRAISKKVKGVVQPQSWFIDIATMSMD Sequence number 12, Sequence length: 160, Organism: Cupriavidus necator H16, PRT MPAVSKPAHKKGDFLVDYEEKVFEDVKAEAGEKALVTFHTVAFEGSIGFVNMLQATRLLRKGFETSILLYGPGVNLGVQRGFPTLGDEAFPGHMNFNKQLTKFMDEGGKVYACRFALQALYGHGEASLIEGIRPINPLDVLDLKLLHRKENALIIDTWTV Sequence number 13, Sequence length: 150, Organism: Cupriavidus necator H16, PRTMYQRILVAMDGGAASELALEQAMIIARAANAEVEVVHVVDDRSPFLDASNLDPVRLIEDLTTAGQSVLAAASGRLAAAGIRHVTRLLGKPMVEGDVAATIAAEANGWGADLVVMGTHGRRGVRRLVMGSVARSVINLAVAPVLLLRAEEA SEQ ID NO: 14 Sequence length: 145, Organism: Cupriavidus necator H16, PRT MRVMVMVKATSESESGKMPSTELLAAMGKFNEELVKAGVMLAGEGLHPSARGKRVRFSGDQRTVTDGPFPQTSELVAGFWLWQVESMDEAVEWVRRCPNPMESESEIEIRPLFEVEDFGAEFTPELREQEERLRAEVEGLANKAR SEQ ID NO: 15 Sequence length: 217, Organism: Cupriavidus necator H16, PRT MSKTTLTISSKTYSSWSLRGWLLARFAGLEFEEVLVPPDDAAARAEILLLSPSILVPCLRHHGVTVWDTLAIAEYLNEIHPKAGLLPEDRAARAHCRAICGEMHSGFAAMRSALPMNLKGHFPGMKVWSRAQADIDRVTAIWTECLTRYGGPYLFGTRTMADAMYAPVVTRFVTYGVMLDPVPVAYCKQILSMPELQEWIAAAREEPDEISELDVEF Sequence number 16 Sequence length: 157, Organism: Cupriavidus necator H16, PRT 17 Sequence length: 68, Organism: Cupriavidus necator H16, PRT MSNHTYKLVEIVGSSPDGCDQAIQSAIAKAGETIKNIDWFEVVETRGHITNGKIAHYQVTLKIGFRVS SEQ ID NO: 18 Sequence length: 417, Organism: CupriavidusNecator H16, PRT MTQSTTLSNLAAGSYTVSAASVLAGSTLLKPQPPSQTVAVTAGGSALASVEYGSPETVQLSLTQVAGGLSEPIFLTAPPGDPRLFVVERAGRIRIVRNGALVATPFLNIAALTTTDGERGLLSMAFDPNYGTNGRFYVYYTDTNGAITIARYNVSAANPDIAAASGTVLLSIPHGTFSNHNGGQLAFGPDGMLYIGTGDGGGGGDPSGNAQNPATLLGKMLRIDVSGASGYGLPAGNPFVGQSGSRGEIWALGLRNPWRFTFDAGLLYIADVGEDQREEVDVAPATSAGLNYGWNRTEGTACVGAATCDKTGLTMPVFEYGHEAGACAIVGGYAYRGSANPVLHGRYFYSDLCTGKLLSFMYRDGVVAEQVDWNVTIPGSVFSFGVDDAQALYVLADPGTSATSGRVYRIDVSDGTP Sequence number 19, Sequence length: 138, Organism: Cupriavidus necator H16, PRT MTPQEITLVQSSWQKVVPIKEKAAELFYGKLFEMDPSLRPLFKGDTVEQGRKLMAMINAVVTKLDQLDDIVPAVQELGRRHVAYGVKDEDYDTVAGALLWTLGAGLGDAFTDEVKGAWTSAYVILAGAMKDAAATAWD Sequence number 20, Sequence length: 105, Organism: Cupriavidus necator H16, PRT MEKKPPPDENTDLPRRGHGSAARAAKTPPPQQSMPGHLIELRPGFFLNPDHIISVRVLPEEEGDVYAVLHLSNGDKQNLTRGEFTAITGEEPRPPARLPQKPLTE

[0098] Accession Number 21, Length: 84, Organism: Cupriavidus necator H16, PRT MATRPFTEFPADEQQQVLQVCRQVGLAAEMFEIFEESREAGVRRISVRRVGTDKTSVYEAGPGSVWVEEFESDLECGLFGQVTA Accession Number 22, Length: 157, Organism: Cupriavidus necator H16, PRT MTRVQPEPPEERPPHGASIVGGIDRDSPGPGPFVMAADTLEGNKVVDPAGDDIGTIDHIMIDVLGGRVAYAVLAMGGFLGIGEKLFALPWSALTLDTRRKCFVLGVERDRLKAAPGFDKDHWPSMADSQWATSIHQYYGISPYWEADRYDPWDDKRR Accession Number 23, Length: 515, Organism: Cupriavidus necator H16, PRT MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALAALEEDQPQVLISDIRMPGGSGLDLLQAIKARHPGLPVIVMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRALEESLREEELDDRLVDAPEILGQAPAMQDVFRAIGRLSQSNVTVMITGESGTGKELVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLELRVKEGLFREDLFHRLNVIRLRLPPLRERPEDITLLARHFLQKSAKELGVEPKRMSDEALAYVSTLPFPGNVRQLENLCNWLTVMAPAQTIEVKDLPREMLEAGTSEPVNAPRPERVAEARAPEYESAPDLADYGGYATTVAEADTATAVRPASVATVVPALASAGWESLLAGEARAMLEAGQPEVMDVLTRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD Accession Number 24, Length: 193, Organism: Cupriavidus necator H16, PRTMTSTTVSATTPSAGTRKPFTLRDATPADVPAIHAIYAHHVLHGRASFEETPPSLDEMQLRFAEVHRKGLPYLVAVRDGEVLGYAYASSYRARSAYRFAIEDSIYIDHRRVGEGLGQALLAELVARCETGPWRQMVAVIACTAGGEGAGSLAVHERLGFRTVGRLEAVGFKHGQWIDTVLMQRVLGVGATTLPA SEQ ID NO: 25, Sequence Length: 355, Organism: Cupriavidus necator H16, PRT MYSLTIEPIGQTIPIAPGQTVLDACLRNGVWLPHACCHGLCATCKVQVVDGEVEQGEASSFALMDFERDNGQCLACCATAQSDLVIEADIEEDADALGLPLADYRAEVVETRALTPTILGIWLRVKDGGRTTFQAGQYVNLNVPGCDQPRAFSLANAPGDELVELHVRRVPDGQATGYLHDQLAVGDELSFSAPYGRFFVRKSAQVPMLFLAGGSGLSSPRAMILDLLAAGETLPMTLVQGARNRGELYYDAEFQALAQAHPNFRYVPALSDEPADSGWSGARGYVHDVLHQLYAQDGNADFRGHKAYLCGPPPMIEACIRTLMQGRLFEADIHTEKFLSAADAQNSARSPLFKI SEQ ID NO: 26, Sequence Length: 100, Organism: Cupriavidus necator H16, PRT MELTPREKDKLLIFTAALLAERRKARGLKLNYPEAVALITAAIMEGARDGRTVAELMHEGTTVLGREDVMDGVAEMIPEIQVEATFPDGTKLVTVHHPIV SEQ ID NO: 27, Sequence Length: 482, Organism: Cupriavidus necator H16, PRTMSARHRLAGACLAAALGGCAIGPNYERPATPEPAQYRIDTGVLSVAADSAWWDSFGDPVLNTLVETALRNSYDVRIAAARIDEFRGQLMVVRSGFFPQVNLSAAATRQRAGTFNGTPFASLDGPRNSYQLLANASWEIDLWGRIRRQAESAEASLWNAEYARRGVVLSLAASVVQGYATLRGLDAQLEIAQQTLKARGAGLDIFRQRYEGGVISQVELSQAENDFYVTEATIPVLRSGIAQTENALSVLLGREPGPIERGKPVNELQPPPVGADLPAALLSRRPDVLQAEQSAIAANAQLGAAQALYLPAVNLSGLFGAIASSPAGLWHSASQVWGIGAGLTQPIFQGGAIRGQVQTASAQREQAMLAYQGTVLSALADVNSALSNGIETRNRLVSLRQQEKSLVVYADQSSARYEGGYSSYLEVTTAQEKLFAAQLSAVQGQVDVLTGTAALYKSLGGGWPSVPQDVREAGMQGDAKVLRR Accession Number: 28, Sequence Length: 325, Organism: Cupriavidus necator H16, PRT MAEINLVEAVNLALAHALEHDPDVLLLGEDIGVNGGVFRATAGLQARFGAARVMDTPLAEGGIVGAAIGMAAMGLKPVAEIQFTGFIYPAVDHIINHAARMRHRTRGRLSCPMVVRSPCGAGIHAPEHHSESPEAMFAHMPGIRVVVPSSPARAYGLLLAAIADPDPVIFLEPTRLYRLFRQEVADDGAALPLDTCFTLREGSDITLVSWGAMVQETLAAADALAGEGVTATVIDVATLKPLDMQTILESVTRTGRCVIVHEAPRTAGFGAEIAAQLADAGLYSLAAPVQRVTGFDTVVPLARLEYTYLPGVARIVDAARKAMAA Accession Number: 29, Sequence Length: 372, Organism: Cupriavidus necator H16, PRTMRIFKLPDLGEGLQEAEIVTWHVKTGDTVAADQPLLSVETAKAIVEIPSPYAGTIAKLFAQPGDIVHLGAPLVGVEGAGEDADAGTVVGSVQVGTHVVNEAAPAGSAAPAAAMAARVKATPAVRALARRLGVDLAMATASGPEGVVTAADVERVASTLAELGTPEQLRGVRRAMAQNMARAQAEVAAATVMDDADIHAWQPGADVTIRLVRALVAGCRAEPLGNAAWYEGQTARRHVLKKIDVGIAADLPEGLFVPVLRDVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIAGRYAAPIVPPTVAILGAGRVREEVVAAGGVPAVHRVMPLSLTFDHRVVTGGEARFLAAVIADLEMAV Sequence number 30 Sequence length: 516, Organism: Cupriavidus necator H16, PRT MGKLQERRRFLGAALGGLLVPALPACGGDDDPPRPPGPVPESQITQAIAQIDKLAADLMASSGVPGMAVAVVRGNQTVYAKGFGRRLVTDPAPVDADTVFQLASVSKPIGATVVARQVGRGGISWDTPVRAHLPWFALAEAEVTEAVTIGDLYAHRSGLPDHAGDRLEDMGFDRQQVLTRLRYLPLHPFRAVYAITNFGMTAAAEAVATAAGIDWATLSEQAIYQPLGMARTSSRYADFAARDNRALGHVRINGAWV QGMGTMPDAQSPAGGVSSSVNDIAKWLAMLLAKGEFAGQRIVDAAALAPALSQQMQSSPPVSGRPAGYYGYGFNVGTTELGLASYNHSGAFAVGAATNLTAVPSLNLAIVTLTNGYPIGVPETLNAQFF DLVQYGAIRRDWGKIYGDALAPLLAPEGRLVGQAPPANPLPARALSAYTGIYQNDYYGPLQVADQGGTLVMTLGATPLTPLSHWDGDVFTFTLNNENATPGTISQAAFSSDRVWLEYYDHEGLGMFVR

[0099] Sequence number 31 Sequence length: 333, Organism: Cupriavidus necator H16, PRT MEVRDGFAGTIGHTPLIRLAGLSAETGCDIYGKAEFLNPGGSVKDRAALYIIRDAERRGLLTPGGTVIEGTAGNTGIGLAHLAHLCAARGYRCIVVIPDTQSPEKMERLRMLGAEVRAVPAKPYADPDNYQKIAGRLADETDNAIWANQFDNLANRQAHYETTGPEIWHATGGHIDAFTCSTGTGGSLAGIARCLKEHKPGVRIVLADPHGSALYNFVKHRELKAEGSSITEGIGTSRVTANLQDTPIDDAVRIDDQTCVDMVYRLLREEGLFLGGSSGINVGAAVALAREMGPGHTIVTLLCDRGDLYMARLFNETWLEGKGLQPIPFRRAGPAG Sequence number 32 Sequence length: 396, Organism: Cupriavidus necator H16, PRT MNLDLLGSLLTAVVLLIGTLVNRSVGVLSRYNIPDPVTGGLLFAIIASVALAAANFRVVIDPGMKPVLLLMFFGGVGLCADLRMLRRGGKALVIFLIILLPYILVQNVVGVAMARTLHPIFGLVSGSITLVGGHGTGAAYAERFAEVNNLQAVMDLSMTVATIGLIVGGIIGGPVAQYLISRYKLRSTARAEGDTEEEAAAVSPITTVGALASLAGILAAVIGGRWIAAQMPTGAITIPGFLFCMMLGIAIRNLLPFAGVRFDDRATDLITNICLSLFLVMTMMALDLAEVALSAGPFLLIIAMQVVFIILYVVLVCFRFMGGDYEAAVTSAAFIGFNMGSTATAMANMRAITSRYGPAPTSYLITPLAGAFFVDLMNAFVLTMLLALPLIGG Sequence number 33 Sequence length: 234, Organism: Cupriavidus necator H16, PRTMSIARPPIAALLAGATAAWLLCMQAAASAPQPPAPAQVQTRDAVTAYEAGNFDQALRGFADAARLGNRLAQFNYAMMLLRGEGTAARPQEALVWLKKAADNQMTHAQYTWGDLYERGELVPKSLEEANRWYALAAQGGHVQAQMALATNYFTGRGVPRDYGQAFMWYSRAASAGDGGAQYIVGSFYEHGEPGVVDRDLEQAKIWYARSAAHGDPGALAKLRSLLEESVRGRAAR Sequence number 34 Sequence length: 133, Organism: Cupriavidus necator H16, PRT MKKLIAMCALVTPMIAASAFAQTTATPATPATPAKPAATAPATPAMPATPATPATPATPDKPAANAQQDKMKACNTQAAGGKDGDERKAFMKECLSKGASKTQQEKMASCSKSGKGKKGDEYKAYMKECLSKAA Sequence number 35 Sequence length: 161, Organism: Cupriavidus necator H16, PRT MELTVVPVTKPEATNFIFGHSHFIKTVEDLHEALVGTVPGILFGLAFCEASGKRLVRWSGTDEDLIDLACENARAIGAGHTFVIFLGDGFFPVNVLAAVRAVPEVCRIFCATANPTEVIAQTDAGRGVVGVIDGASPLGIETTDADVAERKALLRKFGYKL Sequence number 36 Sequence length: 112, Organism: Cupriavidus necator H16, PRT MKTKKAMPALLLAASVLAAPFAMAQGGAKSKPMADPMAASAPAAQNSPYMQVQQWKKGDRLPTEFRDRQYVIDDYKQYNLPAPRKGTRWVGIGAEYYLVAPNGVVQQVGSGS Sequence number 37 Sequence length: 194, Organism: Cupriavidus necator H16, PRTMNRRHFVTRVAGSGLLVATAMAAGCTTTGTSASKDPVAKKQEIDAGVDGALNRLFSANPGARDLAQRAQGVLVFPRVISAGLVVGGEYGEGALRSKGATVGYYSTTQASVGLTAGGQSKAVIIMFMTPEAYNKFVASKGWTAGADANVAVAKVGADGRLDTLTSQQPVIAFVQTNAGLMFDVSVNGSKISKLDI Sequence number 38 Sequence length: 207, Organism: Cupriavidus necator H16, PRT MALLAALLLSGCASTVVTDVTAFRQPGWQNDAPRTYSFERSAEQAAQLDRQTYEQWLATALAGIGFEQVPAKQARYRVSMDFDAAPGIVRVAETVYPDPWYGPWGPYWGPGYYRPYGPWGPWGPWGPGYWPPQTVVRDISVTFSSLRVYFRDAASGKRVYQVTAQNQTENGSLPAVMPYLIRSAFTDFPAESGRPRRVTLEVEKDAK Sequence number 39 Sequence length: 117, Organism: Cupriavidus necator H16 H16, PRT MGRLTTHVLDTAAGTPGKGMSVTLHKIVDNRRETLKTVVTNHDGRCDQPLLEGADFAVGVYELEFAAGDYFRAQGTQLPEPAFLDVVPLRFGIADVNAHYHVPLLVSPWSYSTYRGS Sequence number 40 Sequence length: 73, Organism: Cupriavidus necator H16, PRT MERQFEYGGYAVVVSAVPNAAGGFAAQMRIADAFGEPSGPSYEAIAGDAPTPEGAIALAEAGAMRAIDAGEVN

[0100] Accession number 41, Sequence length: 473, Organism: Cupriavidus necator H16, PRT MAFLVRRLLRSGCAWLFVALVAGAGGAAAQDLQNPPPTFKPEELEALVAPIALYPDSVLSQVLMASTYPLEIVHAARWVKANPKVKGDAAVKAVADQPWDVSVKSLVAFPQVLEPMNDKLDWTQKLGDAFLAQEKDVLAAVQRLRNKAQQSGNLKTTEQQKVIVEPATTAAPGVQTVVRIEPADPQVIYVPAYNPTVVYGSWGYPSYPPYYWPPYPAYYPGAALATGFAWGIGLAAAGAIFGNCNWGGGDVNINVNRATNIDRNFDRTKVQAGGRWQHDASHRQGVAYRDNATREKFSRDVPGGDRRNEFRGREGGAGVSDRAVRDRAGGQERPGAGDRAGDRAGDRAGDRVGDRAGERGGDRAGVSDRAGVSDRGGVSDRAGASDRAGTADRSRGAGERATTVADRGGGGGRDNAFQGVGGGAASQRDFDRGRSSMQSAGGRAGGGFSGGGGRGGGGFGGGGRGGGGRGGRR Accession number 42, Sequence length: 305, Organism: Cupriavidus necator H16, PRT MTSPLSLARSLPGRLACALALALALTVAGMHAAHAQKPFKTPEAAMNAFGEAVATSDDDAMKVLLGARFHDLIPPVGADIRYRFLEAWRISHAVKPEGDSHARIAVGNDGWTLPVPLVKTSKGWQFDTRAGVDEMRIRRIGRNELAVIQAMLAVYDAQREYARTDHDGDGLLAYAGKLASTPGKQDGLYWPTSDGAPPSPLGAAFLTAGQRNPGQGGYYGYHYKLLTAQGPNAPGGAYNYVVNGKLFGGFAVLAWPVRYGDTGVKSFMVSHDGQVYERDLGPDSAGKAAATKTFDPGPGWSKVTP Accession number 43, Sequence length: 115, Organism: Cupriavidus necator H16, PRTMKFPALLLAASMAAGWSSLAYAAGCQYDMQCKGERICQQGQCVFPDAEETAEAGGPAPLPATKRTPAPPPARGCCTVAGKLKLSPAAASDTSLASGDACQGLTASGKPVPGTICN Accession number: 44, Sequence length: 585, Organism: Cupriavidus necator H16, PRT MQRMTESTPARRGARFAMAAMAIAIAAIAGCKKADEPKQQAPAAQQPATPAPAAAAPDTSATPAQPPAQSNLPVAPEAASAPVAVNTSGKKPNILVIFGDDIGQTNISAYSMGVVGHRTPNIDRIAREGMIFTDYYAENSCTAGRSSFITGQSPLRTGLSKVGAPGATVGLQARDVTIAEALKPLGYATGQFGKNHLGDRDEYLPTKHGFDEFYGNLYHLNAEEEPQRPYWPKDKNDPFVKNFSPRGVLHSTADGKIEDTGALTTKRMETIDDETTDAAQKFITKQVQADKPFFVWMNTTRMHAFTHVRPSMQGQSGMPGNDYADGMIEHDGDVGKLLKTLDDLKIADNTIVIYTTDNGPNQWSWPDAASTPFRSEKNTNWEGAFRVPAMIRWPGKIKAGTVSNEMFSGLDWFPTLLAAVGDGDIKERLLKGTSLGSKNAKVHLDGYNQLAYLTGQTNKGARKEFYYFNDDGVLVAMRYDDWKVVFCEQTTPGGFQVWQDPFKCLRVPKIFNLRMDPYERADIVSDQYNDWLGKNAYLTEIGTMKAAAFLQTFVNYPPSQRPASFSIDQVREQVDKKIEESMKKK Accession number: 45, Sequence length: 142, Organism: Cupriavidus necator H16, PRT MTNDLLVERVDVWAATIPDRPGGLAEVLETLRDAGADLQFAIARRTPEDPGKGVVFVTPLQNDREIRAAAQVGFNVTHSLHSVRIMGGDRPGIVAELTRKLAEGGINLRGFSASVIGIQFVAYVSVDSLDDANKAMAILGKG Accession number: 46, Sequence length: 150, Organism: Cupriavidus necatorH16, PRT MGNNQQQAVLALNQLIEAGKDSELACMSGASEVRDAALRDILTSTAESYRASVKELQSMVSAMGGAPADRGSMNGMLYRRWMAFRHMLSPNNDDAVLGMCEHEEDAARREYQSVMMKPLPPEASAALQRQYEALTQHHGRIHAMRGMQIH SEQ ID NO: 47, Sequence Length: 122, Organism: Cupriavidus necator H16, PRT MAIQLNHTIVFSHDKKVSADFLCEILGRPPAEPFGPFLGVRLDNDVTLDFMDAEGDVALQHYAFLVSDAEFDQGFARVRARNLTYWADPYRRRPGEVNTDDGGRRIYFEDPSKHFLEIFTKG SEQ ID NO: 48, Sequence Length: 275, Organism: Cupriavidus necator H16, PRT MSEPSTAARPWSVSDINYQAIDVERVRGDRDLFYLLVSASFIESGSDTYAGNLATYYARVPDAADWLAEHWEAEELQHGLALRRYVEHVWPEFDWERGYARFFDEYSKTCSIDQFEPTEGLEMAARCVVETGTAAYYRALEQASDEPVLRDLTRRISNDEVRHYKHFYQYFNRFAASERLGRLRVLGALARRLLEIKNEDAAIALRNVLRMERGVEAVSEAEVRALNARVSGVIRRNIPIEMTVKMLLRPLHLHRGVERAVRTPLVAMVSRVMLH SEQ ID NO: 49, Sequence Length: 339, Organism: Cupriavidus necator H16, PRTMGFLRWLRWRNAQVHAHGKQEIAELTERVTRIYPRLRLVSRYQQRLRPALEQAQDYVRELVQGLPAPREASMQAWGDDPYVHAFFGAPRDVPLAFSRSPELRAFFEQSPDAPQAFAVLGMAMTERRTLGVALEGDVMRTEVPQTTVSFGDHKVRIIARSEAALREEIVRRMFDQFSMEGLARFAAGKSRRDVLAREQALLVARLRLLQQQGTGMRSVLGGDADASPGEQAALGGQIAANEQELAQLGPETGALDRQLECLAEVLADARSCFSIEQRHLRLSAMNVLLAADSAGPAYDIDLLTAHVPDGDPPLVRSIALVRFARGDMLSPTALIEEAERLL Sequence number 50 Sequence length: 195, Organism: Cupriavidus necator H16, PRT MHPSKLIVAAILAAATLPVLAQPETRVDTVSGVGATRTTGTVKATATVVAIDAATRTISLKNKQGKVTDVQVGEDVRNFEQLRVGDMVTVEYTQALSVALAKQSGMRSSTERETAERAAPGAKPGGVMGREVTVTADVVAVNAKTGMTLKPGKTVDVHVEDPEQLKAVRRGDQVQAVYTEALAISVTPRRAR

[0101] SEQ ID NO:51 Sequence length: 154, Organism: Cupriavidus necator H16, PRT MTEMEQEKVVGVLDRILEAELAGVVRYTHYSFLIFGFGRIPIVSWLREQANESLLHAHQAGEWITTLGAYPSLGIGELLDTHTTDIGAILRESLATEMEALDLYRELLSLVEGRSVALEEYARQLIQLEELHAGEVEKMLRKPGLAATVQPRQG SEQ ID NO:52 Sequence length: 143, Organism: Cupriavidus necator H16, PRT MFKHILLATDGSELSKMAMDAAIGFVKAGGARLTAYTCMEEYPYVASGDAGHPRRKAFEEQEAAHASARLEAVVAAARAAGVTCETDMTTSVPYKGIIDAAARHGCDVIFMASHGRRGLEGLLVGSETQKVLTHCRVPVLVYR SEQ ID NO:53 Sequence length: 84, Organism: Cupriavidus SEQ ID NO: 54 Sequence length: 138, Organism: Cupriavidus necator H16, PRT MLEKKRSYKGFHVALFVPEVIEPGKPGGRVQISTRNEDMGIRFTPDWPHPPATLEEAEEWLFAYAAGIIDCELAGGFGIDLRNE SEQ ID NO: 54 Sequence length: 138, Organism: Cupriavidus necator H16, PRT MDGLTKEELAKLVAQMDAEELRIRAVAGTADSPVVAPTQREHLDDGEVADEKTVQRQGDAMLKHYRMQLADIEAARSRMRSGQYGVCIDCQQAIRFARLQAYPTATRCTACQNLHEHRHPRQAQETRRPASTDLSSPD SEQ ID NO: 55 Sequence length: 223, Organism: Cupriavidus necator H16, PRTMTQANRLSGGHGTRLDIPRHLQVLTVPGLHGSGPGHWQSRWEQRFPDWQRVEQHDWSRPSLPLWAERVSEGVMRAQRVAARGAVLVAHSFGCLASLRQAALDPVGIAGALLVAPADPDKFGVAALLPTYRLPFPTILVASRNDPWMPQRTAFSWGTLWGSELVDAGSLGHINADSALGEWPEGLALLDTLIQRIAQAGDTGSISESSGAWESGIEVVSTIPYI Sequence number 56 Sequence length: 141, Organism: Cupriavidus necator H16, PRT MSRRFLFRLLLAVAALAAAPSLSLSTAAPPNAEAYIIWPHDGTVISGGKFWVRMGLRNMGVCPKGVESPRCGHHHLLVDTELPPLDKEIPSDKNHLHFGAGETDARIELPPGKHTLQLLMGDAKHVPFEPPIYSKKITIVK Sequence number 57 Sequence length: 406, Organism: Cupriavidus necator H16, PRT MWRNLPVVRTAAMAAMLAAAPLPAQAAAAAGPAATEPLLLAGIMPKNTEEQYELSFWESDKKQQLCRANYEAYLKQYPNGRFAVLARARLERLAASASAPKAQAPAPPRSGTSAGGQVTRPPAAAARPASPPAASPAAPPAAPPATASLRPGAAAGGAVSATLRTGEIRDCPACPVLVLPLAGSFTMGSSASDPAEKPPHHVTIGQPFAIGRYEVTVEQWNACADAGGCQRVPTVADSAKNAPVRDVSWDDAQQYVAWLSKTTGKSYRLPTEAEWEYAARGGTASAYWWGDQMRKGNANCKDCGDPWSQDAPAAVGTFAANPYGLHDVNGSVWEWVADCWHSSYKGAPADGRVWNENACGARVIRGGSWREGANYMVSSTRFKYSPSVRQSQNGFRVARDMK Sequence number 58 Sequence length: 80, Organism: Cupriavidus necator H16, PRTMAVIVIKDLPDSMDLDRQAMTAILGGGRTAGGQAWHGLRRQDTARIVDYPGGLPGNRHVMPQEQPLGRLPRKAAGKDAED Accession number 59, Sequence length: 443, Organism: Cupriavidus necator H16, PRT MAENIASTAAPAVIELRGVGKIFRTASQSDRAVLEGVDLTLREGEIVAMLGKSGSGKSTLLRIMAGLVGADRGEVDFRGERLAGTAEGIAMVFQSFALFPWLTVQQNVELGLEAQGVPRAERERRAEAAIDMIGLAGFNSALPRELSGGMRQRVGIARALVTEPDLLLMDEAFSALDVLTGETLRDEMLALWESGRANIRSILIVSHNIEEAVMMADRIVILSSDPGRIRAEVPVALPRPRNRDSAQVRALVDEIYALMTAPAAEARLPAQAPARQIAYRLPDADISQMEAILDLLCEAPFNCRADLPHLADEAGLTDDELLPACEALQLVQLVSIERGDIAATGAGRAWYAAGPQQRKVMFGQQLLGNVALAAHIRRELVASEAGEIREEQVLHELERFLKPAEAERVLSVAIGWGRYGEIYEYRYNSGMLTLPEEELPEAP Accession number 60, Sequence length: 137, Organism: Cupriavidus necator H16, PRT MIARRILGGALVFAAVAASAVALAAPAASKVGKFDVYADALKAGKYDVYTDGAKQGKFDTYTDGARTGRFDPYSEGSRRDGKFDSFTEGSHSFSGEISKKDKFDTFSEGAHTFTGDIADRALDNSRTGDGSLYGYRV

[0102] Sequence number 61 Sequence length: 231, Organism: Cupriavidus necator H16, PRT MSLPPCFTDRREAGQYLGRRLAELGFGAAQLGQPALVLALPRGGVPVAFEVARALDAQLDVLLVRKIGAAGYPELALGAVVEGDAGGGGPHTVVNDDPWARRAVESGSFDAERGR QLDEIARQRRYRGGAPVPERAGRTVIVVDDGVATGATMRAALEGVRMAGAARVVAATPVGSEQGLASLSAADEVICLNTPSFGAVGAFYLDFSQTSDEEALALLRAAAPPPAR Sequence number 62 Sequence length: 170, Organism: Cupriavidus necator H16, PRT MRKRIFWLLPDLASARRTMDDLLLARLENRHIHFVARDGSDMTGLHEANLFQTSDIVHAAEMGLMVGGGVGVVAGVVVAMFPIVSDTPQWGLVGVLAVLGAVFGAWASSMIGSSAPNSRLRAFEKDIEAGKILLMVDVPRSRVEEIETLLANAHPEASFAGLDPAVPAFP SEQ ID NO: 63 Sequence length: 67, Organism: Cupriavidus necator H16, PRT MNWDQIEGKWEQAKGKIKEKWGKLTDDDIAQINGKRDQLAGRIQERYGYTKERAEEEMKDWERDTRW SEQ ID NO: 64 Sequence length: 236, Organism: Cupriavidus necator H16, PRT MNTTAKLIPSTELAQQNPVRFPNESAEYRKARNALLAEEIELRRHIERVAEQRRGLPAGGEVTKRYTFQGERGPVTLAELFGDKDTLVIYSYMFGPQREQPCPMCTSVMASWDHKVPD IEQRVALAMIARSPIDRLVAAKKARGWTKLKVYADVDGDFTRDYVSPEDADAPGYSVFTRRDGKIRHFWSSEMYALSVDPGQDPRGAPDLDPLWTLLDTTPEGRGADWYPKLEYPEGK Sequence number 65 Sequence length: 332, Organism: Cupriavidus necator H16, PRTMSQPDTAYALAELHRESRMGAFGSETRQREIRRIDLSDYARRKAQIADELWQASVDVGFFQIANHGIDLTSVREAFAMTERFFALPDAVKAQYPLQKANNAGWESRAQVRPSTGTPDQKESYQITRPHMAGLWPGDDELEGFRATMLAFEAQCWEVGMKVLSCFAGKLGFASDFFTQAHDPRAPDYQSTLRLLHYYAIPPELRDKLELWRAGAHTFDFCLTLLFQRPGQGGLQVCPGKEMEAQAWTPIEPDEEVITCNIGDMLMRWSDDRLPSNFHRVRNPAPDEYMGPRYSMAFFCQANRGAMIEGPGGKYPPISAGDYLRQRVNANFKGY Sequence number 66 Sequence length: 143, Organism: Cupriavidus necator H16, PRT MSYRMLRLDVRWPWWRLAAAGALSLLAVAGPLRAQPHESGPITIGGSAQVQGPLTVHGKLVVAGHVYASGPLTAAYFTGPATARGLPYGGYLKVFNGPVTVHGDMVVRGDLSVAGPLTVDGPIAASGGIDADGPMREREYGR Sequence number 67 Sequence length: 198, Organism: Cupriavidus necator H16, PRT MAERLIMLDVPGVLYSDRSRTRLGGIPDSGTLRDVRFFDPVALGFVRRLFGVAGARVVVSDAWRRGTPAAILQQLDLQVEAMTPPAAGGHGAEIEAYFAQGAKPVHYVIISCAAPESMPEALREHLVTVDPAAGLTLENFQQALGILGVACPSTVMPAPKMDAGLKAKLAQLQQLARENPARFGGALAPARERTPVPA Sequence number 68 Sequence length: 412, Organism: Cupriavidus necator H16, PRTMSDHIVLTSHARRDKPAEPIHWGAPTAAERGPVIASLANPAQRNVIGTHAGAYAIYRALAVAAGALQRDHRTDLTDTAPAEAIGPHPQWGDPEKIVSLDPWGHLVSTVFADRIAAGVDIRPTIAVTRAHINMPELMSAIAAGRLKPDGDILHANGDVRVTKAAVDPVWYLPGLAARFGIKESVLRRSLFEQTGGMFPELVTRPDLKVFLPPIGGMTLYFFGDVSQLGKPETRVACRVHDECNGSDVFGSDICTCRPYLAHGIEVCIEMAQQGGVGLVVYNRKEGRALGEVTKFLVYNARKRQVGGDRAETYFARTECVAGVQDMRFQELMPDVFHWLGIRIDRWASMSNMKHGALVAQGIEVVEQVAIPETLIPADARVEIDAKVAAGYFTHYTPPDANELALAKGRGLNE Sequence number 69 Sequence length: 289, Organism: Cupriavidus necator H16, PRT MMHMTANPFSAAARARALRTLAAAAALSLALPAMAQQVFPSPDAAAEALGDAIARSDGDALQRVLGKQYQSLVPEGSINRDDVYEFLGAWARHHAVQPDGERRALIAVGQSGWTFPVPLAKQKDGWQFDLRAGQQEVRRRRLGRNELVTMETLLQLADAQQRYAEQVGLGRYATQLISAPGKTNGLYWPAASEQDASPIGPDALAMGPDTPAADAFYGYHYRILPPAKGSDAKYGLIAWPARYGDTGVHSFMLGSDRVFYERDLGPGTASRAKAIRSFSPAGWQRVADR Sequence number 70 Sequence length: 448, Organism: Cupriavidus necator H16, PRTMHRFKHLFAWCLALMLMAPLGPAMAQSKLSNAQLDQLTAPIALYPDALLSQVLMATYPADVAAAAQWSKANPSLSSGDAATKAVASQAWDPSVQSLAAFPSVLDMMGRQPQW VQSVGDAFLAQPNDVMDSVQRLRVQQKAGTLCTTEQKVVTQTTGGTTIVQIEPANPQVVYVPTYNPTTVYGTWAYPAYPPPPGSVFATALVTGIGFGLGVAAVDA MWGGFNWNSHDVNVNRYNNINVNQRLDVNRANVNWQHNPANRGNVPYNNAAVRGRYDQQRQSGLASRQAAQPGQRVGAAGGGVQRQPSERDQARERAAQSFEGRTGQSI PGHAGGVADRSRPGAAGGGVQNPRPAGADRQRADQAQARDRARNANRDSALRDAGNGDRVRQTQRAATPQREAPHARPAGLHQGGGHQGGLGGGGGRLGGGEHFGHGRR

[0103] SEQ ID NO:71 Sequence length: 96, Organism: Cupriavidus necator H16, PRT MNRRYSEEQIRGYLAEAASGVPVRELCARYGFSDASFYGWRARYGQPSQGDARDGRKLRELQEENARLKSMLADALLKLELMRNRTGRRNGTGKDR SEQ ID NO:72 Sequence length: 145, Organism: Cupriavidus necator H16, PRT MFKHLLVPTDGSARADAMAARAMAFASRIGARVTGLHVIPEYHVLTYRLTSLQDTKDTFAAEAARHADTFLAALSHTAAQAGVACETVTATDDHPWQAIIQCAQQRDCDLIVMSSHGKRGLQALLIGSETHKVLTHSTIPVLVFR SEQ ID NO:73 Sequence length: 259, Organism: Cupriavidus necator H16, PRT MTVGSLPAPLVKPSAPRPLLPILVAAIALALPCSITAADDALAPQRADMVREIAAVAAAAAAQSGRHAIDPRVMAVMGQVPRHEFVPDAQKPHAYENRPLPIGHGQTISQPYIVALMTDLMMVKPGDTV LEIGTGSGYQAAVLTGLARAVYTIEIIEPLGRHACDRLKRLAYRQVACKVGDGYYGWDEHAPYDAIVVTAAASHVPPPLIRQLKPGGRMVIPVGAQFLTQYLLLVEKSEDGTVSTRQILPVRFVPLVGKH Sequence number 74 Sequence length: 236, Organism: Cupriavidus necator H16, PRT MTTAILPIPPIAWTEAEFEARLRAMESGYHIHHPFNQRMAAGQCSPAQIRAWVANRFYYQDNIPLKDAAILSNCPERDTRREWVVRILDHDGTDTQPGGIEAWLRLGQAVGLEREALLSHRHVLPGVRFAVDAYVNFARRAPWQEAVCSSLTEMFAPAIHKERLAGWPTHYPWIEAAGLDYFRSRIPLAQRDVDHGLRVTLGHFRSAAQQQRALDILQFKLDVLWSMLDAIQQANP SEQ ID NO: 75 Sequence length: 274, Organism: Cupriavidusnecator H16, PRT MDHTADSFRAFELAGWEDPEVVSQYQEHLSHVTRQSVESLLDEAHVTSGLRVLDVATGSGHVAAGAVRRGAESVGIDFSLAQVQLARKLHPDVHYEQADAQALPFGDASFADAVVNGFMGCHLSDPDAALAEAFRVLRPPGGRIAFTVWDTPERAVGFGAVYAAIRYGSMEVDIPVGPNFLFSDPGHCRTALQQAGFVSPTCRNVPQVWRFSTPDQLFDALAQGTVRAAATLRAQTPKARDEIRAVLRGTVAAYMRGSGFEVPMPAVLAAAVKP Sequence number 76 Sequence length: 225, Organism: Cupriavidus necator H16, PRT MSAVPAVEPLDADPAGLAGVLVVDHPLVQHKVTLVRSEDDTTDNFRRLVRREISQLLTYEATRDLAMETVAIRTPIAPMQSRVLSGKKLCLVSILRAGNGFIDGMLELLPAARVGHIGLYRDPETLEPIEYYFKMPEDIQERMVIVVDPMLATGNSAIAALNRLKEAGVTTMKYVCLIASRPGLRALRAAHPEVGIVTAAIDETLNEHGYIVPGLGDAGDRLYGTR Sequence number 77 Sequence length: 101, Organism: Cupriavidus necator H16, PRT MIPGELMPADGEIELNAGRATVSVTVANTGDRPIQVGSHFHFYETNAALAFDRETARGFRLNIAAGTAVRFEPGQTRTVELVALDGDRIVYGFNGKIMGAL Sequence number 78 Sequence length: 571, Organism: Cupriavidus necator H16, PRTMAKISRQAYAEMFGPTTGDRRLLADTGLIIIEKDFTIYGEEVKFGGGKVIRDGMGQSQRMASDCVDTVITNALIVDHWGIVKADIGLKGGRIAAIGKAGNPDIQPGVTIVVGPGTEVIAGEGMIVTAGGIDSHIHFICPQQ IEEALMSGVTTMIGGGTGPATGTFATTTVTPGPWYMERMLQAADAYPMNIGLLGKGNASQQGPLLEQVEAGAIGLKLHEDWGTTPAAIDTCLSVADATDTQVAIHTDTLNEAGFVEATIAAFKGRTIHTYHTEGAGGGHAPDII KVCGEANVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLDPSIAEDIAFAESRIRRETIAAEDILHDLGAFSMISSDSQAMGRVGEVIIRTWQTAHKMALQRGKLPGDPNDARGGHDNFRVKRYVAKYTINPALTHGIAHEVGSVEVGKWADLVLWRPAFFGVKPSLILKGGMIAAAAMGDPNASIPTPQPVHYRPMFASAGGALHRSSLTFVSQAALAAGIGERYGLAKTLSAVRGTTVSKRDMVHNDWQPHVTVDPETYQVVADGQLLTCEPATELPMAQRYFLF Sequence number 79 Sequence length: 500, Organism: Cupriavidus necator H16, PRTMETQTIRFFHRGQVKEVSDAPITRTVLQYLREDARCTGTKEGCAEGDCGACTVVVGELQDGGDVEFKAVNACIQFLPTLDGKALITVEDLRQADGNLHPVQEAMVECHGSQCGFCTPGFVMSLWALYQQHTPGGEAPSRQTICDALTGNLCRTGYRPIIDAGERMMALPAPTADKLNPKQIADTLRNLKRGETFRYRAQGQEFFAPRSAAEFGAIKAAQPDIRILAGSTDVGLWVTKQFRELGNLLYVGQVEDLNHIEERDGMVEIGAAVTLEKAYAALNAAHPELEEMWKRFASLPIRNAGTLGGNIANGSPIGDSMPALIALGTEVVLQHGETRRTLPLEDLYLAYQKTAMQPGEFVAALRVPVAGPQHFRTYKLSKRFDEDISAVCAFGITVQDGLVTQARIAFGGMAATKPRAAATEAALTGQPWDEATARAGMAALAQDYTPLSDMRATASYRSRGAANLLYRFWLETRAEALSAAAVNVRAIGAGATETATA Sequence number 80 Sequence length: 411, Organism: Cupriavidus necator H16、PRT MNTAFHWRGALWLVCVMTLAACGKDKPAPSAPPAAEVGTMTVAPRDVPIVYDFVGQTQSSQQVEIRARVNGFLDRRVYTEGAVVKAGETMFLMDKPPFAAALDAANAEMAQQVARLKTANADLARVRPLAERNALSQRDLDAVGKQQAAAAAVEAARANVTNARLNLGYTTIKSPVTGVSSFAKKQAGSYIDPSNSLLTYVAKLDPMWVNFSLSENEVLSSRSEQESGALKFPPKGAFDVVVIVMADGSQFPHHGKITFADASFSAETGTYMIRAEVANPDGTLRPGQFVRVKLHGAERTKAVAVPQEAVIQGPRGQMVWVVGADNKAHQRVVDVGQWTGDNWVIRSGLKPGERVVVSGGLRLAPDAPVKATEVASGQKPPAGAAPASAPAPGAPGASSPAQAKSTQSAGSKP

[0104] Accession number 81, Length: 92, Organism: Cupriavidus necator H16, PRT MKTVLTTTVFTLNPGEVTTLSVHAAQRLHVEEARGADVWLTREKDSEDYWLRCGGSLLLRSGDEVVLSVDPRALHPVRLALIAEARRPALALADVPHLVYRSLRRLVRGTEWTPNQDQVTAS Accession number 82, Length: 371, Organism: Cupriavidus necator H16, PRT MWIGVPKEVKDNEYRVGLAPACVRELVSRGHQVMVQAGAGAALGLSDAQYEAAGAKLAADAKAVYDRAQMIVKVKEPQPRELALLRPGQILYAYLHLAADRELTAALLQSGAVCIAYETVTGPDGGLPLLAPMSEVAGRMSVQVAAAYLEKPNGGMGLLLGGVPGVAPAHVVVIGAGVVGANAVQIAVGMGARVTVLDKNIERLRHLGSVYGNRVVTVYSSADSIERALLSADAVIGAVLVPGAEAPRLVTRAMVARMKAGSVLVDVAIDQGGCAETSRPTSHTDPTYVVDGVLHYCVTNMPAAVARTSTFALNNSTIGHAMALADKGWSRALSDDPYLRAGLNICRGSVTYAAVASAHGYAHVPAEQVLT Accession number 83, Length: 416, Organism: Cupriavidus necator H16, PRTMGAPDIIKQHLIDPEICIRCNTCEDTCPIDAITHDDRNYVVRADVCNGCNACLSPCPTGAIDNWRTMLRGQAYPIEAQLLWDELPAEVPLPELADLGEAPQATLGTGTASGAAPDAAIQSVETSRHTSPRAPWSAAHPYVNLHGVRAPVTATVAGNYRLTAEDASSDIHHIVLDFGTHFFPILEGQSIGIVPPGTDASGKPHYIRMYSVASPRDGERPGYNNLALTVKRVDQDHDGKPVRGVASNYLCDLAKGDSVHVVGPFGSTFLMPNHAEASIMMICTGTGSAPMRAMTERMRRNMAHFGGRRQLFFGARNANELPYFGPLLKLPKDFLDIHFAFSRDPAAPRRYVQDAIREAADSVAALLADPNGHVYICGLKGMEAGVLEAFEAVCAGSGHAWPEIEAAMKAEGRLHIETY Sequence number 84 Sequence length: 197, Organism: Cupriavidus necator H16, PRT MAARRSLLSGASGPGPAGWYGSCGNRANNQDPSTVGDIHENAAPPACLARALRVLTALPAGSVLAHGDVTPQAVDTKALPQLGDEWRPDNPYRSGDAHKEALRIGSSAYNQNCARCHGLEAVSGGIAPDLRKLDGDCVSLADAKKKQACMEISQFYTTTVRKGRTRDGRVYMPPFDGVLSQEAVWAIKTYLESRHE Sequence number 85 Sequence length: 428, Organism: Cupriavidus necator H16, PRTMLDLILRHCNLPDGRDTIDIGIAAGRIAAVEPALAAHAADEIDAAGHLVTPPFVDAHFHMDATLSYGLPRVNQSGTLLEGIALWGELKPMLTQDALVERALAYCDWAVARGLLAIRSHVDVCDPRLLAVEALLHVRERVRPYLDLQLVAFPQDGVLRAPGALDNLKRALDMGVDVVGGIPHFERTMAQGAESVRLLCELAADRGLRVDMHCDESDDPLSRHIETLASETVRLGLQGRVAGSHLTSMHSMDNYYVSKLIPLMRDAGVAAIANPLINITLQGRHDTYPRRRGMTRVPELLAAGVPVAFGHDCVMDPWYSLGSGDMLEVAHMGLHVAQMTGQEAMRACFAAVTAMPAQILGLDGYGVAPGCRADLVLLQARDPVEAIRLRATRLRVLRAGKTIASMPPAMAALDLPGRPAQVDFLRRGGGQ Sequence number 86 Sequence length: 301, Organism: Cupriavidus necator H16, PRT MDQDSRQDASDGSVMFADVCDSTRLYEQAGDAAALGAIRQCLALMKSHSAAAQGRVVKTIGDEIMVLFPTAENAMRAALDMQEAVAGLPPIIGAPLAIHIGFHHGPILSDESGDLFGDTVNLAARLASLASRGQIITSKNAVEQLSAGLRQMTRYLYPIQVRGKHQVIELYEAIWQQSIDLTLVAPVDLALARSALLSLRYRETVFEMNTASPPVTIGRDTTMSIVVTDRQVSRFQATVEPRGGRFVLIDRSSNGTHVKIDGESALILRRDEITLRGHGWITFAQPKECIDEGIEFVDEK Sequence number 87 Sequence length: 192, Organism: Cupriavidus necator H16, PRTMVEASELKSGMVIMLEGELHSVTSAGYHAGGGQQGSAVFAKFKSLKTGHIKELRHPGNKLEEVALDHQEMEYLYTDGDAFFMNPDTFEQISFPAETMGAYQKFLRPNMRIPVQLYEGEPVTIAFPPAVEVTIMSTPPGLHEHESSTFKAATLENGMEVLVPQFVKEGDTVRIEVASGKYLERIRRGTRKA Sequence number 88 Sequence length: 260, Organism: Cupriavidus necator H16, PRT MTAAQQLPILANARALVCGIANEHSIAYGCAKAFRELGAEVAITYASEKARQFVEPVAQELDASIFLPLDVTSQKEMDELFARIGEQWGKLDILVHSVAWAPKDDLQGGLLNCSAEGFAKAMDVSCHSFVRMARLAAPLMGDGGTMFTMSYHGANKVVPNYNVMGPVKAALEAVSRYLAYELGPRGIRVHAISPGPLQTRAASGLKSFDLMLDAVERAPLGELVDIMDVGYTCAFLATPYARRLSGETLYVDGGVNIMA Sequence number 89 Sequence length: 249, Organism: Cupriavidus necator H16, PRT MTSKDAAHRAKDSNAGDDAGHPHAPSVSARIRARLEAAQERFHANDNISHHIEPGEMEQLQAEVQARLEDVLRALVIDVDNDHNTQETARRVAKMYLKEIFAGRYAKAPPVTEFPNVGQLNELMIVGPLRVRSACSHHLCPIIGKLWVGVMPNQHSNLIGLSKYARLAEWIMCRPQIQEEAVAQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDDSLRREFLTLLNHNRG Sequence number 90 Sequence length: 140, Organism: Cupriavidus necator H16, PRTMRPRITLITLGVDDLQRAVRFYRDGLGLPTAGIVGEQFEHGAVAFFDLQAGLKLAVWPRTSLAHDTGLQLTPRAQTDFSLAHNVGDKAEVDAVMAQALAAGASVVKPAQDTFWGGYAGYFQDPDGHLWEIAWNPEMLPAD

[0105] Accession number 91, Length: 229, Organism: Cupriavidus necator H16, PRT MPGETPLQGLYPFLHGERQDGARLDAALLASVQQKASDSIDAKRRFFEQNGPVVVAVAQAIALTYRNGGRLFAMGNGGSSCDAAHIAVEFLHPVTAGRPALAAVNLVADTAMMTAVGNDVGFAHVFVRQLVAQARRGDALIGVSTSGNSENLLAAFAKGKEMGLATIGLSGHDGGRMAASADLDHCLVVDSDSIHRVQETHVAIYHILWDLVHTLLAEDRGGLAAGGAR Accession number 92, Length: 122, Organism: Cupriavidus necator H16, PRT MKTVLTTTVFTLNPGEVTTLSVHAAQRLHVEEARGADVWLTREKDSEDYWLRCGGSLLLRSGDEVVLSVDPRALHPVRLALIAEARRPALALADVPHLVYRSLRRLVRGTEWTPNQDQVTAS Accession number 93, Length: 215, Organism: Cupriavidus necator H16, PRT MNCTVHRLAEQALLYSVAPPASLEVQRRIWAMAARAEDWRGVVDVVPGMNNLTVIFDGSADVDALERNLKLAWASGEARNATGKLVEIPVRYGGEHGPDLGDVAAHTGLTPREVVRRHAAGEYVVYFLGFQPGFAYMGGLAPELATPRRREPRVAVPAGSVGIGGEQTGIYPAVLPGGWQLIGRTDAELFVADRDPPSLFAPGDTVRFVAEEIIA Accession number 94, Length: 280, Organism: Cupriavidus necator H16, PRTMTVATPPAETHRIHAEGADLHVRVDGADGPWVILAHALAADHTLWDATAQHLARRYRVVRPDMRGHGASDAPLGPYTMTRLADDVVAVMDALQIPQAHFCGISVGGMIGQTMGLRHPERLLSLALVATNSQTPMEAHPMWHNRIGQAEAHGMAGMADATLGRWLTPAFHEAHRDQVSRIRDSLVATPVRGYVGVAGAIMAFDLAGALSRIHCPTLVVAGEQDQGATVAMAQSIAAAIPGARLEVVPQAAHLVHVEQPERFHAALDAFLGNAACGGQCDVP SEQ ID NO: 95, Sequence length: 153, Organism: Cupriavidus necator H16, PRT MKKLLLSLSALSIAAASSLAMAQGTGVSVGTKADVGASVTTPSPAAAAQGGLNTAGSAATGAAGAAEKGLSTAGAATSKGLSAAGSAADKGLSTAGAAVDKSVGTAADATGGAAADAGTKAKHGKHATKSGKKSKADASVDVSAGAAAGAKAQ SEQ ID NO: 96, Sequence length: 106, Organism: Cupriavidus necator H16, PRT MLITFKSHAAQDLIMMKDLAMTLLGIIGKRLGERGVITVEELPTAIHKLEAAVADAKAHPASSAVEADTGNDEDEEEPLHLGQRAYPFLDMLRASQREGANVMWGV SEQ ID NO: 97, Sequence length: 340, Organism: Cupriavidus necator H16, PRTMQTSNVLVIGGAGFIGSHLVSRLAGAASASGAPLEPGANPDSPIAPDRIVVGTRNIEHAQHLLLLPRVEVVELGLADNAALDDAIGSLGVDGIVVNLVGVLHGERADPYGEAFASAHVEMVRQIAASCLSTGVRRLLHMSALGADSNGPSMYLRSKGDGERVVRASGLDWTIFRPSVVFGPDDHFLNLFAHMQQMAPVVPLACAHARFQPVFVQDVVQAYLNAMVNPATIGHGYELGGPRVYTLEELVRFAGRASGHPRPVIALPDALVQATIMEHMPGEPMLSRDNLDSMQLDNVLEHPVAPELNLHPVSLESVMTDALAGRNRRDTAMTHMRASVHR Sequence number 98 Sequence length: 197, Organism: Cupriavidus necator H16, PRT MPLLRLFLAWLAMAPCVAAAQVVPASQGEIAVSELKRPNVVVVLRHASAPGVGDPPGFKLDDCSTQRNLDANGRQQAARLGSSWKAAGRFRPTRVYSSAWCRCQDTARLIDLGPVRVQPLLNSFFGADAQRREAQTAQLSRFIDGLDPRGGPYLLVTHQVVITALSGHGADSGGGVAIELQPGGAPRRARVLPNAGLD Sequence number 99 Sequence length: 114, Organism: Cupriavidus necator H16, PRT MSEDLAAQSCTPCRGGVPPLTPAEAEMLLQQAPGWELVDSATRIERRFTFGNFAQALAFVNGVGQLAETQGHHPEISFGWGHATVSWRTKKIKGLHRNDFIMAVKTSELAEGIG Sequence number 100 Sequence length: 197, Organism: Cupriavidus necator H16, PRTMIRHSSSLLAAALAATAFAAGSANAQRPPAPVGVAEEAVISGKIVDIDPESKAVLVQGPRGNVVELVAGDEARNFGQIRKGDILTVARGAALVAALEPVDSKATALAESDRTTRAAEGGKPGIMREITTVTAQITKVDPAKRLITFRGPRETLRTVKVQDPAIDLNGIKQGQMAKIVYREVVAITVKAAAAAN

[0106] Accession Number 101, Sequence Length: 161, Organism: Cupriavidus necator H16, PRT MAQDIFLKINGIDGESQDSSHKNEVEVLAWDWSIEQQSTMHAGSGGGAGKATVSDLSFEHFIDRASPNLMKYCLTGKHINEAVLVVRKAGGNPLEYLKLTMTDVIVTKVSPKGSVDDEVRMREKVALSFSRVKQEYVVQNAQGGSGGAVTAGYDIKGNKEA Accession Number 102, Sequence Length: 292, Organism: Cupriavidus necator H16, PRT MTHSPTILLVEPTFYDVSYSINPWMDPAAWARDPRGMHRSAMQSFDALREALGRAGFAVEVAPGAPGLPDMVFPANAAVVLDGRALLARFRYPQRRGEEAPFADIFGKLRERGLLDEVALLPEGCFQEGAGDCIWDAGRGHFWAGFGPRSSHEAAVAVSNYFGKDVVALELATEQSYHLDVCFCPLSGGEVLYYPPAFSEAALRELRARVPARLRIEASADDLRHFSVNAVNLDDQVVMTRTTPHLRTELGRRGYQLHEVDLSPFMLSGGGAYCMTLRLDRRSGPGIAAVAA Accession Number 103, Sequence Length: 361, Organism: Cupriavidus necator H16, PRT MKSTARSLFLDASDVARLVATIGPQAAIVDLAGQVRQAFLRWHDFDKSARLASHSARGVIELMPVSDGIQYAFKYVNGHPRNAQHGMPTVMACGLLAEVETGFPLMLADLTLATALRTAATSALAARAMARPGAATMALIGNGAQAEFQVLAFQAMLGVREIAAYDIDPAATTRLMRNLGGVPGLSIRRAESIEAALAGADIVSTVTADKTRATILTPEMVRPGMHLNAVGGDCPGKTELHPDILRRARIVVEYEDQTRIEGEIQQLPADAPVTELWQVLAGHAPGRAAADEVTVFDSVGFALEDYAALRWLYTAAQAHRAGRAVELVAMPPDPRNLYGWMMAQADGDEAGLATPRQAAVA Accession Number 104Array length: 257, Organism: Cupriavidus necator H16, PRT MLHLPPFAPTRSLELVEARLARRSRVKRLVIALSAGVVALLALVPAASHAQAPVVEANPAPAAPAPATPAGTMAPTPGATMPAAPPADTMPPATQPAPGIAAPATAAPPPTASPAPAPTGTPPAAPAPAPMAAPAQPRPPLTTPPPPPPRDAMVQRTAGSVAYICGGVAEDEQVALQSEARKYNMSLLFTQGARGEYLSDVDVKLMRGGKEVASFVADGPRCLVKAPSGTYNVRATYQGRTKTATVSTGGKNAQMRW Sequence number 105 Array length: 108, Organism: Cupriavidus necator H16, PRT MIAKRTIRRWLRQSAMLAAGGALLAACAAPAPTGPQGARGTPEGCAADKLADDALVGKPEAEAIGLLQGCAWRLGERDGQQFPGTMDYNPQRRTLGVKAGKVVWVRRG Sequence number 106 Array length: 303, Organism: Cupriavidus necator H16, PRT MQSLHPHRSATRRALAAATLLLAAGAAHAADGDTLKKIKDSGVISLGYRESSIPFSYTDGKEVMGYSHEILLQIVDKVKSELKMPGLQMRLTPITSQNRIPLVQNGTIDIECGSTTNNLERQKQVAFSNSMFVYGIKMLTRKDSGVKEFTDLKDRNVVTTAGTTGERLLVKMNGEKAMNMNLISTKDHGQSFLILETGRAAAFVMDEPLLYGERTKAKSAADWVVVGAPLQTENYACMFRKDDPSFKKLADGVIADLQTSGRAEKLYNKWFMSPIPPRGINMNYPLSADMKALFAAPNDKAYQ Sequence number 107 Array length: 375, Organism: Cupriavidus necator H16, PRTMRPVLHFMARTLRPASLLAIVLLAGTGSAPAALPLDQVRLPPGFRIEVLTQDVPGARAMAMSPSGTLFVGSRSEGKVYAVTDALGARPRVRTVATGLRMPVGVVALHDGSLYASSVSKIVRIDHIEASLDQPPAPAVVSDRFPTDGHHGWKFIAFGPDGYLYVPVGAPCNICAPDENRYANIMKMKPDGSDLQVVARGVRNTVGFDWHPATRELWFTDNGRDRMGDDVPDDELNRATAAGQHFGYPYCHAGTVADPEYGSQRPCAEFVPPVARLGAHVAALGMRFYTGTQFPAAYRHNVFIAEHGSWDRSEPSGYRVVRVALDDAGKAVRQEVFAQGWLRGGRPWGRPVDVLVAPDGSLLVSDDLAGAIYRIRYRP Sequence number 108 Sequence length: 165, Organism: Cupriavidus necator H16, PRT MRIHSKKWLIVPVAAAAVLAGCAAPYDSGYNTGYNAPPGYQTPNTSQAPAGVYYGRVESIEPITSTQGSSGLLGTVIGGAAGGLLGHQIGGGRGQTAATIGGAVVGAVAGNQIEKRAGSNTQTAYRVNVRLDDGRIATVTQSSLGNLQVGMRARVANDMATPY Sequence number 109 Sequence length: 86, Organism: Cupriavidus necator H16, PRT MENAEWRVEEYRGMDVYVLVSPHGQDSIGRWGYEVRVSQEGADPADAGDTELLASGPQQFATRHAEAVAAFGAGYALVDRLLGPAE Sequence number 110 Sequence length: 93, Organism: Cupriavidus necator H16, PRT MSEPLHDEALVNLYLERISALSVSAFDGADVSGELDAVMREAVTKCQAAGGPQAHGTLTVLATRLREHADAAEREDQPLVRDTFRRAAELVRT

[0107] SEQ ID NO:111 Sequence length: 95, Organism: Cupriavidus necator H16, PRT MGPFLFLAEIPMRTRIAVLLAFAVVGAAACSNTPAERPGSAQTIGKPNPASVNCTQRGGKLQIVSTPAGQSGICIFPSGKQCEEWALMRGECTPG SEQ ID NO:112 Sequence length: 175, Organism: Cupriavidus necator H16, PRT MNSVIANGARRLMLALGAAMLSACTTLMPVPEGTRLVGQPQAAVQAAFGPPTDVFQLRDGTSRWIYTKQPLGQYAYGADFDRNGNLTRFSNVLSTLELYKAQVGTWTKQDVAEHFGMTRLPVEYYPLMRREVWSYRFRHEDVWPSLYHFYFDDAGILRQTQITPDPMYDPDERRR SEQ ID NO:113 Sequence length: 626, Organism: Cupriavidus necator H16, PRTMAHPDLSSMAWRGALALALTALLGACGTQGGGTAAPAAPAAPSATTPPAAMPPAPEVASGYRPGMSTVYAQRHMAAAANPLASEAGRAILRQGGSAIDAAIAMQAVLTLVEPQATGIGGGAFIMYWDGKNVQAFDGRETAPAGATENLFRPDGKPMEFSEAQIGGRSVGTPGVMRALEMAHQKHGRLPWAKLFQPAIDLAEKGFPISQRLYTQVAADKFLANSPEMAAYFLDAQGKPKPAGTVLKNPKLAQTLRDVARRGAGVLYNGPIARDIVAKVNGGSNAGSLSLADLGSYRAKQRVPVCTDYKRWKK GMPPPSSGGIAIAQILGTLQALETKNPKYALAALKPQGVNTPAVMEANPDAVHAISEADRLAYADRGLYVADADFVPVNVTGLVSPSYLATRAELIGDKSMSKAQPGVPPGTAMAYAPDRSPPRVSTSQIVAVDGRGGAISMTTTIESYFGSHLMVRGFMLNNQLTDFSFVPSENGKPVANRVQPGKRPRSSMAPTLVFDRESGQLVATVGSPGGSQIIEYVSKTLVGMLDWNMDVQAAIGMGNFGSRNGPTEVEKGLVSPGLVQALQQRGHQVAEIEMTSGTQAIMRKQGPDGKVVWAGGADPRREGVALGD Sequence number 114 Sequence length: 328, Organism: Cupriavidus necator H16, PRTMQRRHLLRLLATASAAAAAGISFSAMAQAGYPTKPITLVVPFPAGGTTDIVARIVADKLGQQLGQAVVVDNRGGAGGSIGTAFLSKAAPDGYTLGIATASTHGINPAVYPRLSYDATKDFTTITNLASVPNVMSINPAVKATDMKSFIALAQSQPNKLAYGSAGNGSVSHMMGELFKMSSKTELLHVPYKGVGPALNDALAGQVQVLFDNLPSSLPFIEGGKLRALAVAAPKRVAALPNVPTFAELGLGEVNDAAWFGLIAPANLPADIQTRLHTAAVKVLALPEVKAKLEKLGATPVGDTPAHFAAQIKSEVAKNKRVAAAARISLD Sequence number 115 Sequence length: 80, Organism: Cupriavidus necator H16, PRT MSDIGEWKKKVYETHEVQVQVKQRSQSEWSYTVRVCRPGTNIRAAGTLTETSRITEHYKSREAAEVAGFAHGERLAKQLA Sequence number 116 Sequence length: 491, Organism: Cupriavidus necator H16, PRTMAGRYFDEMLDWRTDGAQWVIQAGQALPEGAVRPHYRHFADWLARQSEGTMGNKRAADLIFRRVGITFAVYGDKDEANSGTERTIPFDVIPRIFPANEWALLEKGLRQRVAALNRFVHDIYHGQDIIRAGVIPPDQIYNNAQYRPEMLGVNVPHDIYAHVAGIDVVRAGEGEFYVLEDNLRVPSGVSYMLENRKMMRLFPDLFANRVAPVAHYPDLLLDMLRGVSPQAIADPTVVVLTPGMYNSAYFEHAFLAQQMGVELVEGSDLFVEDDHLFMRTTQGPQRVDVIYRRVDDFLDPLVFRHDSSLGAAGLLSVYRAGNVTICNAIGTGVADDKSIYPYVPDMIRFYLGEEAILSNVPTYMCRRPDDLQYVLDHMEELVKETHGAGGYGMLVGPAATRAEIDAFREVVKARPEQYIAQPTLALSCPTYVESGIAPRHIDLRPFVLSGKEVRMVPGGLTRVALREGSLVVNSSQGGGTKDTWVLER Sequence number 117 Sequence length: 140, Organism: Cupriavidus necator H16, PRT MSARATPSLPESPAIVPAVLRCLAACAATGALLLAAGCAELPRDVNGSPPTARLAPNAIPPAPLTADDKQKLDTLNQQILREQAAAIALDQQAAAARAAYGYPNTSWNLYYGGWGGGGHWGGVSVGMPGWGWGGYPYGWW Sequence number 118 Sequence length: 84, Organism: Cupriavidus necator H16, PRT MSVLMLVNIDTATTDEEIQEFIGRYGFPACDEIERVPDGTRPAVSLHFNSVDGAALRLLQPRINHLFWKQRKVDAMVLTERFE Sequence number 119 Sequence length: 278, Organism: Cupriavidus necator H16, PRTMIANARMYSVSAPVEQAWRALLVHVADRAGVDLPYVEHPAPAPVNALWARPDCGCVFMCGYPYASAPVTPQLLAAPVPALARYGGRPVYFSEFIVRADAPVQALAQTFGARLACMLPESNSGYNAPRHHLMRLAPAGTRALYRPAAPTPTPREVIDAVIGGDAEAGVVDSYVLDLLRRFEPGLAGRLKTLEVTPAAPIPPLVASAQADPHECAYVRAALLTLHEGATGTALSMLGLARFAPVQPADYAVLPELAREADQAGFALTDAGPGTAKHDN Sequence number 120 Sequence length: 348, Organism: Cupriavidus necator H16, PRT MKTAVKPDVEAAVKPAVKPVLRRMAMRCMLACIAACAPWLAAAPALAQPPGYPAKPVRIVVPFAAGGPADVLGRAVGEGIAKATGQSVLVENKAGAAGTIGVDMVAKAAPDGYTLALVPVGNIAVNPTLMPNLPYKPADLAPVAMLATAENVLVVNAATPVKSLAELLKLAGQKPGELSFASPGAGSQAHLAGELLQLDAHVKLNHVPYKGISPAMTDVVGGQVTMMFAQMSAALPYIKAGKLRPLGVASAKRSAVLPDVPTIAEQGFPKFEALSWYALMAPAGTPAEIVRKLSQHVDAVLADATLKEKLATLGMEAAGGTPQQLATTIQKESARWAGVIRQRHITID

[0108] Accession number 121, Sequence length: 223, Organism: Cupriavidus necator H16, PRT MRILIVEDAPMLGDGLRRGLQLLDYAVDWFSTGTEADHALGVVHYDAVVLDLGLPGEDGMSLLARWRARGSRIPVIVLTARDAIDSRIGGLDAGADDYLVKPIALDELAARLRAVTRRAAGLSSPVWKHGALEFHPAARQAYWQGKPLELTSREAMLLELLLAHPNRLLTRDFLRDKLYDWQGGVESNALEVHIHHLRRKIHPKIVRTFRGAGYTLGAAEDCT Accession number 122, Sequence length: 493, Organism: Cupriavidus necator H16, PRT MLAAALAGAMPARAADPLRIGSKRFTESYILGELLTQAAGPPGAAQHQPGLGNTAIVFEALKAGGIDLYPDYTGTLAAEILKLPPGAGLDAIQRALAPMGLGAAIPLGFENTYALAVSDARAGALRRLSDLAAQPQFRLGLSHEFLGRADGWPGLASRYGLPQRPVGLDHGVAYEALAAGQVDAIDIYSTDAKIRKYKLRVLEDDRHYFPRYDAVVVYRLDVPQRFPQAWQALERLAGRVSADDMIAMNAAAEIDGQSFDAIARAFLAGTAGQRATHDGAEPRSRLAAALFGPDTTRLALRHVGLVAGAVGAATLVGVPLGMLAARRRRTGQAVLGVVNVLQTVPSLALLAMLIPLLGRIGVWPAMVALFLYALLPIVRNTATGLEQVPQGMRDAGRALGLRGGQVLRYVELPLALPVLLAGVKTAAIISVGTATIAAFVGAGGFGERIATGLALNDTALLLAGAIPAAVLALVVQAAFETLEWALRRHRDAR Accession number 123, Sequence length: 358, Organism: Cupriavidus necator H16, PRTMDIAATRGFLASPSRRAIAALVLAGLALAGCSTPATAPESQAAVTQAAQPAAQALASWRDGAARQALLKFVADVTRKGSPTFVPPEERVAVFDNDGTLWSEQPLYFQFFFLLDQIRAAAPQHPEWRNNPAFKALMANDMEGLMRNEKQLAGLVVTANSGMTTDEYDRTIRDWLAKSRHPRFNRPYTELVYQPQLELLSYLRANGFKTYIVSGGTIEFMRPWSQAVYGIPPEQVIGSSQAVRYLVRDGKPVLVRDPKLEFVDDGPGKPVGIYRHIGRRPILAVGNSDGDLQMLEYTTGGDGPRMGVLVHHDDAEREFAYDRQSKVGKLDKALDAARAKGWTVVSMKQDWQQVYPTAGAK Sequence number: 124, Sequence length: 324, Organism: Cupriavidus necator H16, PRT MSARDDVLALQQRMGESIVGQQRMIERLLLGLLADGHLLVEGLPGLAKTRAIKMLARNLDARLSRIQFTPDLLPADITGSEVYFTEGGKGEFRFQAGPVFANLVLADEVNRSPAKVQAALLEAMEERQVTVGGTTHKLEPLFLVMATQNPIEQEGTYPLPEAQMDRFLMHVSVGYPEADAEADIVKLAREEEAGGGAVGGAQAVRLAPEAIFAARAEIHGIHVSDAVERYIVTLVQATRAPKPVDDDLDKWIQIGVSPRGSIGLDKVARAHAWLHGRDFVTPEDVQAVVHDVFRHRLILSYEAHAAGIGADAAIDRLVQRVAVA Sequence number: 125, Sequence length: 163, Organism: Cupriavidus necator H16, PRT MQSKNTICLWYDRDALDAANFYAETFPDSAVSAVHRAPGDYPDGKQGDVLTVEFTVAGIPCLGLNGGPHFKHNEAFSFQIATDDQAETDRLWNAIVGNGGQESECGWCKDRWGLSWQITPRALSAAITDPDRAAAKRAFDAMMTMKKIDIAAIEAARQGKSPS Sequence number: 126, Sequence length: 84, Organism: Cupriavidusnecator H16, PRT MSRLLLGNIEPGTSDEEIAAFLEKYGFPPFDSLEHEEGDGSQPAVLLTYGSLDPMVLGKLQQRIHHMYWKKRELTAAILHDRFA Sequence number 127 Sequence length: 307, Organism: Cupriavidus necator H16, PRT MREINQQRLRYFREVLEQGTIRGAAENLNTSPSVITRQIRLLEDELGTTLFEREARGVRPTEAAHLLEFWRDYRSHQEKLEDQLHALKGLRQGQVVVLSEGYVDALVNEVLAPFCAQYPLLDIGMEILGVDQVLDAVAESRAHIGLAFNPPPHPRIEYLASSSQPVGLLVRPDHPLALRGGGAASAKDILSWPLAMMPASFGVGHAAKMLEFAENIRIRPTLVTNSLTALKQFVTYENYMTLIGGEFAAYREIAAGTLTVVPIDHPLFRGTQARVLAKAGRPLPAAALKLLEHILRDMPMFAQGGQ Sequence number 128 Sequence length: 155, Organism: Cupriavidus necator H16, PRT MRLFSRALLVISFALCAFGPAVLAQQVGAQASLYDRLGGLKGITIVVDDFIDRLVTNKTLNRNNPAINAGRKSSPPYLKFQVAQLVCEASGGPCKYTGKPMKESHAHLNISEREWDVMAEEFKKSLNKFKVPAAEQKELFDIVGKTKADIVVRK Sequence number 129 Sequence length: 537, Organism: Cupriavidus necator H16, PRTMSNPKLTLESVSYVLPDGRTLFSELSEQFDDRPTGLVGRNGIGKSVLARILGGELEPSAGRCVRSGSVHYLSQQAAHAVQTSVAGLAGVQSMLDALQRIEAGSAAAQDFDDVGEHWNMPQRLRQELERNGLGHLQAHTPASTLSGGEAMRVALIGALLSDADFLILDEPSNHLDRPNRLALIAQLQRWQRGLLVVSHDRQLLEGMARIVELSSLGLRSYGGGYSFYAQCKAQERENALQQLDQRKLERKREEQSLRAQRERQERRQARGNRQARDANQASILLGRQKERSQASAGRLRLQQAATREALDARVRDAAQQVEDDAAIVLLALPVSQAAQRRVAELDEVDLPFVPAATRRISLMLTGQQRVGVVGPNGCGKSTLLKLLAGQLQPLAGRCAVHAERAYLDQQLAGLLPQRSTIEQMLAANHTAGEAALRTRLALLDLDAQKATAPSGLLSGGERLKAALACTLYADPPAQLLLFDEPSNHLDLPSVQAVETMLRGYQGALVVVSHDDVFMGSLGLTHRLAATPDGWKLEPW Sequence number 130, Sequence length: 463, Organism: Cupriavidus necator H16, PRTMSNLTSHWVKRIAGSAKPAYLLIPDLIEEALADGQLRARDRLPPLRDLAEALQLNYTTVARGYSEARKRGLIDTKTGSGTYVRGRAPAMPLRGGTGAEMSMNMPPEPPAMTARLRSAMAQLLEAADPYTLLRYQDFGGTPADRSVAAKWLRRWVPACSADTVLVTPGIHSALVALLSQLARPAQTICADALAYPGIKAIAAQLNIQLQALPGDEDGPFAHAFEAMCKTQKPAALYCNPTIQNPSTRTMPQARREQLAEVALRYSVPIIEDDAYGMLPLRAPQTFAALAPELTYYVTGLSKCLGAGLRIAFLHAPTARQAQRLAGTLRATTVMASPINTLLATSWIKDGTVDDMLKAIRAESAARQLIARHVLEGLAYDADPEGFHLWLPIPAASAWSPSELALHLRSRGIGAVSAAAFSTDGNPPDAIRVCLGGPGERDEIEESLRIIAETLDNPHHLHSAML

[0109] SEQ ID NO:131 Sequence length: 91, Organism: Cupriavidus necator H16, PRT MEQKTARLTLLIDPNKKAAFERLCAQQDLTASQVIRRLIRDYLAQHGVQYVPSGVGTDKAEAKGKERPPVGASSRRDAAPPAKRRTTRRGA SEQ ID NO:132 Sequence length: 174, Organism: Cupriavidus necator H16, PRT MWKILRQIAHTGIVTEPAPRGAADEAASIQRIHRELLDILGQALTIRQVDAGSCNGCELEIHALGNPYYNIEGLGIKFVASPRHADMLLVTGPVSRHMEEALLRTYAATPEPKLVVAIGDCGCTGGIFGESYASCGRVANVIPVDITVPGCPPPPIEILRAILSAVRQTGATRR SEQ ID NO:133 Sequence length: 282, Organism: Cupriavidus necator H16, PRT MTDSSVKSNLRNTWESAAPGWAKWEHVFATNLSGATDTLIDMAGIRPGMRVLDLACGAGSQSIQAAMRVGAGGEVVASDISGTMLEHVRRNAARAGLQNIETLECAVDELDETLAPFDAAMCRLGLMLFPSPGEALESVRR VLKPGARFAALVFTTPANNPFMAQPMAILLRHAGKSPPAPGQPGIFALGGDGVMERLMNDSGLGDVKTQHVRAQLVLPSASDALQLMQEAAGAYRAVVADLDVAARLRAWNEVYECLKQFDVGEGFKTELELIIGSGAKPG Sequence number 134 Sequence length: 144, Organism: Cupriavidus necator H16, PRT MFQHILLPTDGSELSKKAINGGLELAKAIGARITAYVCLEEYPYTPFSEIVVEAPQAFKDRIENQAKLYLKEVENLATAAGVTFDADMSTFAVPYLGIIDAAERHGCDVIFMASHGRRGLSGLLLGSETQKVLTHTDIPVIVYR SEQ ID NO: 135 Sequence length: 153, Organism: Cupriavidus necator H16, PRTMNTQHLPGGHAPAVAHSQHRQRLALAMLAAAGMALAASWRPAHAAAEEMVIRTMGPVSYVCGGVAEDERQALAAKEKDFNMGILFTQGANGEFLSDVAVRLMRDGQEVASFRAAGPRCLIRAPEGSYNIEASYKGQAKSIKVSTGTRNAQLRW Sequence number 136 Sequence length: 286, Organism: Cupriavidus necator H16, PRT MLGKLTSFLRTRSRAKLEHYAIPDALWARTVATLPFVQRYGSEDLAALRELATLFIADKEYSTAHDLPLADDMVASVAVQACVPILKLGLPWYRGWHGIVLYPGEFIIRRTVQDEIGLVHGVVEEASGEAWEHGPVILSWPDVHTPGAGIDSDHELPADTYNVVIHEFAHKLDMLDGEPDGVPPFSRVLHPDIDAEAWAETFLTEYDRFAESWEARDEADWEHPERLPPALRIIDPYGTEAPSEFFAVTSEAFFVEPTGLARHWPVVYQSLAAFYRQDPASM Sequence number 137 Sequence length: 284, Organism: Cupriavidus necator H16 H16, PRT MELISWNIQWGRGADGHVDLGRQVDTLRGIADADVICLQEVTRGFGELRGSPGEDQVTELTALLPGYHLLYAPGVDRRTATGGRKQFGNLIATRLPVREVFRHALPWPADPQVASMPRVALEASVEVSTRRLRVICTHLEYYSSHQRAAQAEALRTWHAEACDHARRPGRSEKWPGPFTPEPRPAEAVLCGDFNSKPDDIAYRRVLEPFDDATPPWRDAWLHTHPGQPHAPTCALYDKEQWPEPPFACDFMFVTENLAGRIRRCEVDADTASSDHQPILLSLDL Sequence number 138 Sequence length: 283, Organism: Cupriavidus necator H16, PRTMLGKLTSFLRTRSRARKLEHYAIPDALWARTVATLPFVQRYGSEDLAALRELATLFIADKEYSTAHDLPLADDMVASVAVQACVPILKLGLPWYRGWHGIVLYPGEFIIRRTVQDEIGLVHGVVEEASGEAWEHGPVILSWPDVHTPGAGIDSDHELPADTYNVVIHEFAHKLDMLDGEPDGVPPFSRVLHPDIDAEAWAETFLTEYDRFAESWEARDEADWEHPERLPPALRIIDPYGTEAPSEFFAVTSEAFFVEPTGLARHWPVVYQSLAAFYRQDPASM Accession number: 139, Sequence length: 83, Organism: Cupriavidus necator H16, PRT MALNFPNPSRSYDAARHCVCFWGYDNAREVAFQVTDEILLRLSHQGAAEESALLATFDHHRDQILQLARDLYNPGERTTYTIS Accession number: 140, Sequence length: 140, Organism: Cupriavidus necator H16, PRT MTHRALRPAKVLLLAALLGSGLTFSAASMAQVSVNIAIGVPPPAPIYEVVPAPRSGYVWMPGYWDWDDHHHKHAWKKGRWERERPGYIYESPRWIQSRDGWVLVPGRWDQGGYKDKGQKDKGHKDKGYHCPPGHAKKGEC

[0110] Accession Number 141, Length: 67, Organism: Cupriavidus necator H16, PRT MASESRNNLKAFVQTAPQAGRYVWVIALVDFGAQQIRRAIVSDDTFTTSDAARVAGEAQLKAMAEDH Accession Number 142, Length: 121, Organism: Cupriavidus necator H16, PRT MAGAPARAEQGNGLSAEYERCMAKAISTADILGCAAVETRVQDVALNSAYQGLLRRLEAPRTGQLRVAQRAWLEFRQANCAYVANPAGGTAARVAGATCMARMSAARVRELRDFATEAAAR Accession Number 143, Length: 187, Organism: Cupriavidus necator H16, PRT MPRQPCLASLRHDAAMRRIFAPIVVLLTAFLAPIGSVAAQTLAQSPGMADSVVSRLVAWRARTDAPVALPALTAFEWDHFSVTDSPAGDAIAHCGRAGLMPCGPQLQPQPGDRVQVLRFERAGTQVYTERIMAGDGAFAEPLPQRVPRAAATLVSCPATDGRQLWCLQGKARGREAPRQPQPFLDGG Accession Number 144, Length: 314, Organism: Cupriavidus necator H16, PRT MRPCGATPDPALTAILELRKVRKQYGDTVVVDDLDLQVFRGQCFGLLGPNGAGKTTTLRLLLGLTTPMAGTLTLCGEPIPERAPQARMRVGVVPQFDNLDPDFSVIENLRIFGRYFGLSSAEIERRVPMLLEFARLENRADAQVRDLSGGMRRRLTVARALINDPDLLVMDEPTTGLDPQARHLIWERLKSLMASGKTILLTTHFMEEAERLCNYLCVIDGGRKIAEGKPHELIDSQIGCDVVEVYGDELDTLRGSLTPLAQRTEMSGETLFCYVREPAPLLAALHGRNGVRYLHRPANLEDVFLKLTGREMRD Accession Number 145, Length: 78, Organism: Cupriavidus necator H16, PRTMDLQGFTYYRDHWYEASATSLGVGGPWQGEVTICAKAADGAPLKVFDHLVPGQFFSEGEAWQHASDWARKQIDARFG Sequence number 146 Sequence length: 258, Organism: Cupriavidus necator H16, PRT MGFDSHFQWTSAACTDVGRVRERNEDACLDLPSQDLWAVADGMGGHAVGDFASQAVVQALATLPPQPRLEDRVDATRAALQGVNLALVDEAARLGVRCIGSTVVVMLAGDRRCACVWAGDSRLYRLRGGQFARLTRDHNQVERLLARGLITPEEARHHPAQNTITRAVGAAPTLAPEQLLTEVADGDVYLLCSDGLSNEVDDDDIAAVLAQQDVAQAAPTLVQMALDAGGRDNVSVVVVLRAADPYGSDKTLVNPELDA Sequence number 147 Sequence length: 250, Organism: Cupriavidus necator H16, PRT MTGIVRIDDEVLGFEEFVRLLKLTGQFEGLVEQMVRDKLTVHAAKRQGVVVTPEEIQERADQFRRVQGLHRAADMNHYLDALNISLDEFEAFVTDSLYQERMMEQVCSEAAVQAYFALNSPRFDSIEVSHIVVDSEGKAKELISYLQDDPDAFAEMAREHSIADTRERGGEIGKVLRGSLKTDIEAKVFNAEPGDLLGPFPSADSFFEVFLVREKHPATLDSEVAVEVRRLLREDWLMARAQEHVIEAR Sequence number 148 Sequence length: 479, Organism: Cupriavidus necator H16, PRTMNAQPDAAPVPLEPSEVVAALWRDAGLPAEALAHLKLDGAEPVLPSSFAVGTAAQASLGAAALAAAALWHSRTGRWQDVSVDMRHAITEFRSERYLRVDGGPAPELWDKLAGIYRCGDGRWVRLHTNFPHHRDGVVRLLGCANDKAAVQAALEKRDAEAFETAASDAGLVVAALRSFEEWDRHPQAAALRGLPPVTLERIGDAPPQPLPAPASADAQPLSGVRVLDFTRIIAGPVAGRTLAAHGADVLLVTASHLPSIPPLVIDTGRGKRSCQLDLRDPDDKRTLHKLLHGADVMVQGYRPGGLAELGVGPEAAARARPGIVYVSLSAYGHVGPWSHKRGFDSLVQTATGFNDAEAQAAGSDMPRPLPAQVLDHAAGYLLAFGAMAALHRRAVEGGSWHVRVSLAQVGQWLRGLGRVPDGGLKAPEQKIDDVSDLLEAVPSGFGMLTVVRHAAHLSETPARWTLPSEPLGTHAPEWLPRG Sequence number 149 Sequence length: 195, Organism: Cupriavidus necator H16, PRT MAARALALAAALGITAAQAAPLEYTIDPAHTTVYFSASHFERSSVRGRFGKIDGRLVYDADSGAGALDVTVDLASVDTGNRTLDGVLRSAQFFDTAQHPVARLRADRFLMEGGRLAAVEGELTLHGVTQPLRLQAERFRCGEVTLFGVSRQVCGGDFRAEVPRSAFGMTRFLPEVGDTVTLQVAVEASPAGSTPR Sequence number 150 Sequence length: 208, Organism: Cupriavidus necator H16, PRT MERMRTATAVTTDPTDSLDRIVTAPDARRRGLLKAPGLFALGSLAVISLGESMPAWAQTQSGSTRDDINILNTALGLEYQAIAAYQVGAESGLLQKPVLATAVKFQDHKAHAQVLAGTVSKLGGTPVVAKKASEYTFPTDKLKTQADVLRFAAGLEKGATAAYLGVLPNFHNRELTRAAGSILGDEAMHWAVLLHALGEDPVPGAFVG

[0111] Accession Number 151, Sequence Length: 200, Organism: Cupriavidus necator H16, PRT MSLTLFQRFALAEIAPTRWKNGGGNTREIAVWPPGAGMDDFMWRLSVADIGQDGPFSAFPGIDRQIVLLEGAGVTLRADDASFSHRLDHIGEPFAFSGDTSLQATLIDGVTRDFNVMTRRGHCRARVEVFRQGFVPGTGGHAVCLLAVSGAWQAQDAGLLLQAGEGALCGAAEPPSTPSFEPVGDAALCLCVTLETEPGQ Accession Number 152, Sequence Length: 322, Organism: Cupriavidus necator H16, PRT MRNHSRIRGRMAVQAAVLVAALGAGAAQAQNTQVTLGMSGWTGFAPLTLADKAGIFKKHGVDVDIKMIPQKDRHLALASGAIQCAATTVETHVAWNANGVPITQIVQLDKSYGADGLAVRSDVKSFADLKGKTIGVDAPGTAPYFGLAWMLKKNGMTLKDIKTTTLSPQAAAQAFVAGQNDGAMTYEPYLSTVRQNPDKGKILATTLDYPMVTDTLGCAPKWLKENPKAAQALVDSYFEALEMIRKEPAKANDIMGAAVKQTGEQFAKSSAYLRWQDREANRKFFAGELASFSKEAAAVLLEIGVIRQVPDVTALYDARFLK Accession Number 153, Sequence Length: 153, Organism: Cupriavidus necator H16, PRT MDDPFRRTAFGAALFYKDPLAALDWLERAFGFERVMVIRDQQDQLVHSEMRFGDSYLMVGSEWADFTASPASIGGKNTQTLHVHLREGLDQHCERARAAGAVILREPQDEFYGDRTYTARDAEGHVWTFGQTVRKVTREEAEQASGLKIDGWA Accession Number 154, Sequence Length: 99, Organism: Cupriavidus necator H16, PRT MNPNVREFFIQGITKEGKTFRPSDWAERLCGVMAQFRPEGDSGDPRLTYSPYVRPIFAGNVKCVVVDVRLRDIEPKALDFVLNFARDNNLQLVEACSLESequence number 155 Sequence length: 464, Organism: Cupriavidus necator H16, PRT MELTNRLDATTIAARVAAGRLDPAEVVAAFQARIAARNDQLNAVFEQRQELVDADLAQLRARLAQGERPLLAGVPVIVKDVIWSQGRRVTQGSQLYRDFIAPADAIAVERLRQAGAIVLGMTSEFACKGLTTNKVYGLTRHPLDATLTAGGSSGGCAVAVAAGMAPLALGTDGGGSSRRPPAHAGVVGFKPSYGAIPDSVGFPHAFNGIQVIAPITRTVADAELMFEALAGADPRDPDTLGFSLGAARPLHTLKIAVSPRLGLDTPVDDDVAQAFEQAVARLQGAGLTIERADPAWPKGADEAALMPLQHVGLANLYGEAWRRDPELFDPDVARQIERGLAWSGADVARAREASHQIALAVAGFFTRYDLLLCPTTPCVAWRNDRLGPERIGGVAVEPRAHAVFTPFFNHALAPAISVPCGHGRDGLPAGLQIAGPRGADRNVLAAARLAESVLAPLLNPAG Sequence number 156 Sequence length: 213, Organism: Cupriavidus necator H16, PRT MLDHLRRIVVEGPVGSGKTSLAQRLARTLQAQELPDSARRSPFLEPFYRDPARHALALQLWCLTQRAVQLQQWQAGVLAGQRMVTNFLMAKDRLHAALTLSDDELALYDAIAARLDLPPQRADLVIVLQATPSLLRERIVRRGEPGEAGIDEHYLQRLAGAYGELFHRYDDAPVMVVDTAHFNPVDNDDFRTLLSRIENMRGRKAFLNLAAP Sequence number: 157 Sequence length: 192, Organism: Cupriavidus necator H16, PRTMKRRTFLSASAGLALGSLALGACTTTKPDAPTDKAARRRELDSGVDGTLSRLYSSVQGSRELGNRARGILVFPKTLSAGFIVGGEYGDGSLRSGGATRGYYRLVSGSVGWQIGAQSKSVILMFLTQDAYDKFVRSEGWVAGVDATVAVATVGASGVLDTKTAQQPIVGFVMTNAGLMAGLSLEGSKITKLDL Sequence No. 158, Length: 148, Organism: Cupriavidus necator H16, PRT MKTRPYLVLCGLLPVLAACSSTPQPPQVTPVNATIKLGRVTEKTFLTRLDVAQVPPYYGGGTSVGVGAAGGGGGGGVGVGFAFDLSRLFNKPAPVQQVDLFQYKVRTMDGTIVAANAPAAPGLEPGACVRVIYPDGSQEARLAPSNEC Sequence No. 159, Length: 505, Organism: Cupriavidus necator H16, PRT MKAQEFAACWDSLGLPRPWREGTPAGAVTVRSPVDGEAIGHVPACTPAQADALIARAHAAQTTWALLPAPARGEIVRRFGEVLREHKPALGRLVSLESGKILQEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPQHAMRETWHPYGLCGVISAFNFPVAVWAWNAALALVCGNGVIWKPSEKVTLCALAAHGLLERVLAAAAPQHAGISAVLPGGRMLGTHLVSHPAVRLVSATGSTRMGREVGVACAGHFKRSILELGGNNAAIVAPSADIELAVRAITFAAAGTAGQRCTTLRRCFIHADLMDTMASRLITVFDRLPVGDPLADGTLVGPLIDTAAGDAMANALAACRAQGNIVTGGERLLADRYPHACYVRPALVMTDAQHDTMLTETFAPILYLMPYTTLDEAIALNNASPHGLSSCIFTESMREAERFLSSAGSDCGIANVNIGTSGAEIGGAFGGEKATGGGRESGSDAWKGYMRRATNTINYGDSLPLAQGIRFEI Sequence No. 160, Length: 798, Organism: Cupriavidusnecator H16、PRT MSFRLKQTSALIAAAWLAFAPLSPQAATPAKPAATALGWPRNFDAANDHVELYQPQIETWDGNRLSGRAAVAVGEKSGSPTYGVVHFSATGDIDKSSGL VQLTRITVDSVEVPTRPDAADRVRQALVSRLPANGLTVPLDQLQASYAVSQQLAKAGRVVVRNDVPQILFATTPTLLVLVDGEPAWRAVPGTQYERALNS RALLLRESGGELHLKAAGYWYRSESAGGAWEVMTTVPKALESAATKAAGMKPDTMLPADGKRPARAPAILFATQPTELVMTSGAPQMTPVSGVSLLTMS NADHAVFVDPGANQYYVLVSGRWFRAPMLTGPWEYVPGAQLPADFARIPATDPKANVLVSVPGTPQAREAEIAATIPQTATVSRSKASLAVAYDGAPRFT PVTGTSLSYAVNSGTTPVIEVDSSHYYAVWWFTAPAPPSGPWRVATEVPSAIYTIPPTSPVYVTYVRIYSVTPETTVVVGYTPGYMGVVVSADGTVVY GTGYVYPPYPPYVGAVYYGYPATYGYGAGFGIGMAEGFAGFGFAAGAFWGAASPYWGPYWWGGAYNWNYVNVNQANFYGRWGQGTVTHAQGWNAWTGTEWRGTA GAGYDPATGARYQGSRGGAFNPYSGNYAAGRQGSFANPSTGREGAARGGVAGNTYTGNYAAGRQAAGYNAQTGRVGAAEAGIAGNTQTGQHTAGSRGFVA NPDKNNAVVWNNGNVYAGHDGSVYQHTDNGWQKHTPSGWEQVQPGNDATARLEQQRQAREIGQRFQGTAQQWQGRGGWGGFGGRAGGFRGGGFHGGFRR

[0112] SEQ ID NO: 161 Sequence length: 201, Organism: Cupriavidus necator H16, PRT MQRNHKPQILKQFLAASAALLVAGGAFAQASAPAPSAAPAGKPGMGHHQHHGGGMWMKAIDTDGDGAISKAEADAWFNKLDTNRDGKIDKAEMDAHRKATMAERHARMQAAFDEKFKAADKNGDGALTKAEANAGLPRLAKRFDQLDANRDGKLTRNEIRAGMEKMHRDHSHRGDRGPRGQAAPGSQPAPAAPGAPSTSGG SEQ ID NO: 162 Sequence length: 116, Organism: Cupriavidus necator H16, PRT MTIFPRPAVLLRLALLAAALCGAQPALSADSAAPATDAALRARSQAAACTSCHGPAGRAPAGSTIPPLAGRPQAELAAQMQAFKAGTRPATVMHQIAKGYSDDQIAAITAWFAAVR SEQ ID NO: 163 Sequence length: 167, Organism: Cupriavidus necator H16, PRT MKQCPHRKLYRGLGVLAAAIALTPALIPAQAMAQNKPMAYPAKGQSQQQQASDDGACYSWAKQQTGVDPAQAANAPPPAQAQSGQRVRGAAGGAAAGAVVGAMAGDAGKGAAVGAAAGTVGGGVAHRQSRRQANAANQQAAANTSQAMGSYYQAWGACMQGRGYSVK SEQ ID NO: 164 Sequence length: 322, Organism: Cupriavidus necator H16, PRTMRRTYSRLAHAVMASAAVAATFAAAPAFALDSVKVMIGANPGGGFDQTGRSLGAAMIAAGQAKTASYDNKGGAGGTIALTQFVNTDKGNPNALMVVGAVMVGAIETNKPPVTLKNATPIARLAFADTMVITVPASSPIKSVKDLTTQLKANPGSVSWGGGSKGSIDHILAGLIAKESGVDPKKINYVPFQGGGEASASIMGGHVTVGIAGVSEFLPFIKSKGMRALAVTSKDRTADIPTTLKEQGVNVEIYNWRGVYGAPGISAEQRKAMIDAVVKATESQAWKDTLQKNDWTPFLLTGDEFGKFVDSESARLGGGSLRRELGVAK Sequence number 165 Sequence length: 254, Organism: Cupriavidus necator H16, PRT MNAKASRFDPARRRWLGAGAAAALVLAVPLPRAARAQGPPAASREATAQRIGVIGSGRIGGTVGGLWVKAGHPVLFSSHRHPEALKPLVEELGTLARAGTVAEAIAFSDVLFLATPYGALPQLSRDHADAWRGKIVLDATNAYEHRDGPITEEARRDGIGITTARYLRGARVVRAFNFMGAAQFASEHHRAGGLVAVPIAGDDKAALDVAAQLVRDAGFEPVVVGPLASADRFAPGGPLFRQVGTAEEFRRKMGAR Sequence number 166 Sequence length: 116, Organism: Cupriavidus necator H16, PRT MIRPTVQENAARYADCIACNAASAAALKCAAACLEEQDVRKMARCIALDMDCAGIAQLAASYMLRNSEFATLVCEDCAEVCKWCKEECEHHDNEHCQECARACAACMEQCLKMTA Sequence number 167 Sequence length: 107, Organism: Cupriavidus necator H16, PRT MKRQFLGLLARVYVLQFALEVPATVAMLADMLLEYGFQVDIGSVRPLLRALQMEGYLESVLRDGIGRVYLLTERGREELQASRQRIDDLYRRLHAGQPATDEVPQQA sequent number 168Sequence length: 253, Organism: Cupriavidus necator H16, PRT MPFEFIDAAHAGQAGSDPLQDELALLDLAAQGRQVAQLWEAPLSLVVPRTYLRHAALETARADFAQQGCPVFLRMSGGGLVPQGPGILNLSLAYTVEQPPGARSDAVYLHLCEVIGDALQSLGVDTHWQAVAGSFCDGRYNLAWGPPEAARKIAGTAQYWRRAPAAMQTADGQRHLVLAHAVLLVSAGPVQINARANAFEAAIDSGRHYDAGKVVSVREALLASGRAVDDDAALMAQVSDALRRSVGQTPPPA Sequence number: 169 Sequence length: 161, Organism: Cupriavidus necator H16, PRT MKTLFLVRHAKSSKDDPSLPDRERPLNDRGLQDAPEMGKRLAERKLKPDLLLSSPALRALTTAQLIADELGYARKDIALDDQLYATSAEQLLAVVRALDKKLDCVMLFGHNPEFTDFAHRLSDEITDMPTCAVARFSFDTRAWEDVGEITPSKVTLESPKK Sequence number 170 Sequence length: 135, Organism: Cupriavidus necator H16, PRT MQAHPLRLSPGDDLRAALEDVLSRLKLHAAFVIQGIGSLSVAQLRFAGAEDATELRGDLEILTLAGSLSPDGAHLHMSVADSRGRVLGGHVARGCTVRTTAEILLALLPEHRFAREYDQSSGFMELVVRNEPPLD

[0113] SEQ ID NO: 171 Sequence length: 146, Organism: Cupriavidus necator H16, PRT MTIVVLPSDGSPYSIRAARRLVSPGLFERPLEVHLVHAFPDISGRAQAYFSKPQIDQWANEAADNAFQAIRPLLKAAHCAVTEHACIGEPAPRIVDVVLACRADVIVMGTHGRGAFLSAVLGSVAARVLASSPVPVLLVPKHHGHD SEQ ID NO: 172 Sequence length: 296, Organism: Cupriavidus necator H16, PRT MELRHLRYFQVVAQELNVTRAAERLHMAQPPLSRQIRQLEEEVGVALFDRVGRGLRLTEAGRFLLEQTTQLTQRLEETIEGTRRIGRQDKRWFGIGFVPSVLYGVLPELIRELRESDRHDQQVEVGLAEMITVEQIEALKAGRIDLGF GRIALNDPAIRQQVVMAEPLVAALPARLPAPRRKALTPQALAAQPFILYPARPRPSYADHLLSMFRAQGLQLRVAQEANELQTALGLVAAGIGITLVPASIQRLHRDDVRYCPIDAPGFVSPVIMSYREGDTSPFLARAVEWVMRQAQ Sequence number 173 Sequence length: 443, Organism: Cupriavidus necator H16, PRTMPMPITFSLLRVVLLAGATAALAACGETAKLPSEAGFGPTPQLPPPNQTAIPTVKIAPAIGWAAGQRPVPAAGMAVTAFADKLDHPRWVYVLPNGDVLVAESNAPPKPDDGKGFRGWIMKKVMKRAGAGTPSANRITLLRDTNGDGVADQRSVFLKDLNSPFGMALVGNDFYVAATDAVLRFPYQPGQTRITAAPQKVVDLPGGPLNHHWTKSLIASRDGSKLYVTVGSNSNVGEHGMDKEVGRAAIWEVDPQAGTHRIFASGLRNPNGMAWEPVTGMLWTAVNERDEIGSDLVPDYITSVRDGGFYGWPYSYYGQHVDARVKPPAPDLVARAIVPDYAVGAHTASLGLAAAGGNNLPARFSEGMFVGLHGSWNRRPLAGYKVIFVPFSKGRPNGAPFDVLTGFLDEDGNARGRPVGVAIDKQGGLLVADDVGNTVWRVSGAK Sequence number: 174, Sequence length: 68, Organism: Cupriavidus necator H16, PRT MNEETFNLSIRKFLKVVGVSSQREIEQAIARAMADGTIAGNEHLPVSVTLELAGVKLQARFEGEIQLE Sequence number: 175, Sequence length: 177, Organism: Cupriavidus necator H16, PRT MQSRTPSRCIGELCVAAVVLGSLGACATLQPEQTGKQMIGSPESAVRSTFGSPTETYRLADGTSRWIWSRQPLGHQVYAADFDAGGKLTNYRQMLTETEIYRAQVGTWTKKDVQEHFGLPREPIQYYPLMKREAWSWRMYKDGLQTAHFSCYFDDAGVLRQTMIIVDPLGGDARHSR Sequence number: 176, Sequence length: 360, Organism: Cupriavidus necator H16, PRTMNAKRIAGALIAAAHVACVAAVLLPGTAQAQPAQQTKLRVAGNLVATGLIQQNKEQPFFENFARNTGLPIDADYKPMDVLGIKDSDGLRVLRSGLFDIVSLRLAQVSRDEPFFLGPDIVGLSTDYGSASAKAVDAYRNAFDKRLQERYGAKLLGIWPFGPQVVFCKSPIGGLADLKGKKVRVYDQSLAKFIEKLGGIPVTIPFGETQQALERGVTDCAITGPSSANTAGWPEVTSYFMPVGFQIALNAYAIKLSRWNALPPDQQTRLAEAFQRFERDTWSYSRELFEDASRCNVGKEPCKTGKKFAMKEVPVRPADLQLVRDALTTVSLPTWTEVCDKSYPQCSAIWKQTIGPLVGVK Sequence number 177 Sequence length: 121, Organism: Cupriavidus necator H16, PRT MLSPLFPRSLPTALVMALSVTCASAALAAGQDEAPKPRGKDEAPEAPVACMKAVKAALPNPASFKWVSGKARKVAEDAYSVVADVEYLAQDGAVRKAKVQCDVLRAPGNQFVVPKVRLPQH Sequence number 178 Sequence length: 302, Organism: Cupriavidus necator H16, PRT MSKIGFIGLGVMGKPMVQHLVDGGHTVFAHTRSGVPQDLQDAGVKACASAEEVAKQSETIILMLDTPDVERVLFGEKGVADGLADTAGGITVIDMSSISPIATREFARCIEALGVDYIDAPVSGGEVGAKAGTLSIMAGGKQEVFDRVLPILQLMGKNITRVGEAGDGQVAKVANQVIVALTIEAVSEALVLAARAGADPARVREALMGGGFASSRILEVHGERMIKRTFDPGFRIALHQKDLELALSTARQLGVGLPNTASCQQLFNVCSGLGGAGWDHSGLVRAIEHLSSFAIGDEPPAA Sequence number 179 Sequence length: 510, Organism: Cupriavidus necator H16, PRTMTKSSSSTDKPDLTQGVPLADLADGGMVTGQVGGKPVLLVRRADELFAIGATCTHYGGPLADGLLVGDTIRCPWHHACFSLRTGEVLGPPAQDDLKCWRVEQQDGKAFVRDALPAVRPPRLASARLPESVVIIGGGAAGTAAVETLRREGYSGPVTLLSADRSLPYDRPNLSKDYLAGTANADWLPMRPPTFYADHDIDVRSDNRVVKLSPEQKSVTLSDGSNVSYGALLLAVGAVPIRLDVPGATLPHVSVLRTLADCDALIARLGTARRCVVVGASFIGMEVAAALRTRGLEVHVVAPEAHPMERVLGATLGDMIKALHESHGVTFHLGATVAEIQPDRVRLSTGAELAADLVVTGIGVRPDVALAQDAGLALDKGVAVDEFLQTSEPDIYAAGDIARWPDPGTGQRIRVEHWVVAERQGVVAARNILGQRQRFAAVPFFWTQHYDVAINYVGHAEQWDRFDVDGDPAAHDCTVTYWGKDKRLAVATVGRDLASLRAEVAFEQETTGH Sequence No. 180, Sequence Length: 468, Organism: Cupriavidus necator H16, PRTMNMRPDPTFHASPELAISAPAESFAYTVLLSPDASQPDALAVIDAKPGSPTYSQVVHTVPMTNKGDELHHFGWNACSSSLSPLTGHAFLERRYLIIPGMRSSRLYIIDTKPHPTQARIHKIVEPDEIFRKTGYSRPHTVHCGPEGIYVSTLGGGGPDGTDGAPGIFIMDCETFDVLGRWEIDRGEQEKHYDFWWNLPRDYMVSSEWALPPQFENGLVPEDLLGNRYGHKLHFWDLRARRNVQTIDLGANHQMALEVRPAHDPIREYGFVGVVVDTTNLEGSIWTWWREDGKFHAEKTATIPPEAADASLLPPLLQGFGAVPPLVTDIDLSLDDKFLYVSCWGTGEMRQYDVTDPRKPRLSGSVHIGGIVRRTPHPNGKPFAGGPQMVEISRDGKRVYWTNSLYSTWDNQFYPDGIPGTQVMAHAGPDGGLTLADDYWVEFPEGYRAHQIRLEGGDCSTDSFCYPSVKA

[0114] SEQ ID NO: 181 Sequence length: 224, Organism: Cupriavidus necator H16, PRT MCTNYAPVQSRILREIFGVEPPPGLYKAETYPDYAAPIVRAADDGTRSGALASFGMVPRPRIPPGARRFDTTNARSETVGERPAFARYWRQGQLCLVPATAFYEFAYPEDAAPGGPPGKPVRWRVWLPGEPAFGIAGLWRSWPDQSLSFTMLTINSAQHPVMQRFHKPGEEKRSVVIVPREQWDDWLACRDPEVARTFLTLWPADAMRAEPAPKVQGAPIPDSE SEQ ID NO: 182 Sequence length: 190, Organism: Cupriavidus necator H16, PRT MKQVLPPLAACIAATIALAGCAGSGMSSSSAASASGTSASSSARAAATLQPKSGSNTGGTVTFQQQPGGVMMTAAITGLPPNSVHGFHVHEKGDCSAPDAMSAGGHFNPTGKPHGQMTMPDHHAGDMNNVTADASGNVRVSMLLPDLTVGTGANGVIGRAVVVHKDPDDYKTQPTGNAGGRIACGVVAAS SEQ ID NO: 183 Sequence length: 159, Organism: Cupriavidus necator H16, PRT MPFQLQSTAFAPGGEIPAEHTCEGADISPPLAWTGLPPGTASLALIVDDPDAPDPAAPKMTWVHWLLYNLPSHAEALPADIGKAGLPAGTRDGLNDWHRAGYGGPCPPIGRHRYFFKLYALDVALPDLGMPDKEALERAMRGHILATAELIGTYQKLQR SEQ ID NO: 184 Sequence length: 334, Organism: Cupriavidus necator H16, PRTMPEGPRPAQPESRHRWLATAAALAVAAGVARHLLFRAVERKAFVTPTDETKDAAAPDISALTVRHATFASGNRILHAAFMPAADPEAPALMVCHGDNESLPDWAPVQAMLAGAGIASYVFDYSGYGRSSGRPSVRRLREDALAAYRQFLAATPQAKRRVVLGHSLGSGILLEVAQRFDPQPDGYVIAAGFSSARLAAVQTGRIPAWAAWLLPDPWNNAARASRLERPLLVVHSRDDAVIVPPHAERVARAARHLHALVMLDAMPHDAAILPEHIEAFWPPILRFVRAQDGDAMQALLAGNDAKYEGDPPGNADQAGTSTSRTGTLATASGERRQ Sequence No. 185, Sequence length: 253, Organism: Cupriavidus necator H16, PRT MSRWVFLRGLTRESRHWGSLPARWEAAGLGRPLLPDLPGNGALSAQPAPWSVPEMVTAVRRQLSATGAHGPYRVLAMSLGAMAATEWASAYPEEVGGLVLVNTSMRPFCTPAQRLRPRNWGKLLRVAQRWQDRAYCERTIHAITCARTDTLARDLETWQAIAADAPVSRQAALAQLLAAARYRAPASAPRCPALLLASAADRLVNPVCSARIAQAWDAQLLTHHWAGHDLPHDDPQWLCNAVAGSVILTLSGG Sequence No. 186, Sequence length: 136, Organism: Cupriavidus necator H16, PRT MQPSPTFRRFTISRRFALACACAALLAACAPDSVRNIQAKGFNAYLDSLQTACPNMVVGSSNISDWLRTSGSRGDDNYVYWLDQTSRLYYQRISPTQYRDSVSAALGGKSDSRALDCIVRNLPPDRPTRPPGGKLL Sequence No. 187, Sequence length: 60, Organism: Cupriavidus necator H16, PRT MTKPATQKHQTPASREQQRKDELIERELDEGLKETFPASDPVAVDTHAPRKPTPEPKPAK Sequence No. 188, Sequence length: 157, Organism: Cupriavidus necatorH16, PRT MRPAWRIATDTPDYTADDASGAGAKATGGRWNRQGTPLIYAASSIALACLETLVHLGSGGLPLNRYLVRIDIPDELWAAAQKLSVDTAPVGWDAIPAGKTSLDTGEAWTAGGTSALLLVPSIVIPEEHNVLVNPAHPDAARLAYVKVRRWQYDARLV SEQ ID NO: 189, Sequence Length: 111, Organism: Cupriavidus necator H16, PRT MSLPHLESGQIASVLPLGARLDQTATQALFKEGPLEVMRLVLKAGKHVPPHAVDGPMTVQCLEGEVRVRVDGAERQLHQGDLLYLGPSVRYDVTAISDASVLVTLVLPGSR SEQ ID NO: 190, Sequence Length: 138, Organism: Cupriavidus necator H16, PRT MSQIEIVKAFFARYREHDINGMLAMFSQDARIDYVPMRLEGPAGDAGRTIWRALMDGFPDLSNQVTAVYGDDDQRTAIAEVTITGTQARDVLGIASRGQSFSLPHVFIVRMDTDGRIEFMRAYWDNAAFYAALTPAAT

[0115] Accession number 191, Sequence length: 138, Organism: Cupriavidus necator H16, PRT MGKTVLVTGAGSGFGKGVALGLARSGHNVIAGAQIWPQVTELRNAAELEGVRLTVIKLDMLDAIDRQRAFQHEIDVLFNNAGIMEAGPVAEIPIALVRRVFETNVFAALEMAQGFARQMIRRGSGKIVWTSSVAGLVKVPWVAAYAASKHAVEALCASMREELKPYGIQVATVNPGAYRTGFNDTGMESMDQWWEDGKQAMRPRMKRPLNNQHDPQEMIDAMIDVITAASHRYRTIKPDSAAEMVRREQRDEWDVIV Accession number 192, Sequence length: 83, Organism: Cupriavidus necator H16, PRT MLETMLNQFPPLEQEAFRETCLRNGVAPDGFTVTAVEDAAGAGPPRRRTVSVGFGREIRQYNGNLGAQWTVDFEDDLRSRVFG Accession number 193, Sequence length: 134, Organism: Cupriavidus necator H16, PRT MKTVTSVAAALVLSCLTVAKPAAAAPEASVTPLVKEALPEYPGKEAEMIIVEYPPGSADPVHRHDAHAFVYVLEGSIVMGLKGGKEVTLKPGDTFHEGPNDIHTVGRNASKTQPAKFVVFLLKKKGAPILTPVK Accession number 194, Sequence length: 250, Organism: Cupriavidus necator H16, PRT MKIVVIGGTGLIGSKTVAILRQGGHEVVAASPRSGVNSITGEGLQEALAGTQVVIDLANSPSFEDKAVLEFFETSGRNLHAAETKAGVQHHVALSIVGTDRTPENGYFRAKVAQEKLIEASSIPYTIIRSTQFMEFIGGIADSSAQGNIVSLSPGLFQPIAADDVAAIVADVALAPPRNGIVEIAGPERAPFNEIVARYLKAVGDPREVVRDPEARYFGGRVEEHSLVPLGEARLGRIGLEEWLRQSQQR Accession number 195, Sequence length: 278, Organism: Cupriavidus necatorH16, PRT MAYDSILVHLDSSPQARQRLEVAGRLAAASQCRLIGMYAGFIPDPAWFYLMDGAQQYVADDLARRKQVREDVRAQFLAATQAMPVRAEWRAAEQGAVASVLREAREAGLVVAGQYDPGNTEGPVARQLLESLLLETGRPTLVVPCAGTFPSLGTRVIVAWNGSREAARALHDAVPLMAGAQARIFCAQTAAKEARADATPVGHAARVLERHGVAVEIEHGPGGADLTIGELILSRAADFDADLIVMGAYGHGRMRELVLGGVTRTLLDSMTAPVLFSR Sequence number: 196 Sequence length: 267, Organism: Cupriavidus necator H16, PRT MPNSTVNIERIKPGQGKATPNRSLERGIAVLRAFRAGASMLGNSEIAERTGLSRSTVSRLTQSLIETGMLEYVPQCRAYRLGVPVLSMAHAMRDGSQVLRVAAPMMAEVAMRHKINVGLAVAEGDEMIYLESFRYNRRQSLRTVVSGQRIPMALTSLGRAHLATLAPEAFNAMMRAMADKNRGRGWGELRREIDAAVSSVHEKGYCVASWQPQVVAMATPLRFDEYAVHVLNVSVSTHQDAGAVEAALAKPLMELAAAIQERMPRVE Sequence number 197 Sequence length: 134, Organism: Cupriavidus necator H16, PRT MLQNSDVAARIPAQDLARARSFYSSKLGLEPVEERPGGLRYKCGNSYFVLFESAGSASGNHTQMAWEVDDIQATVSELRQLGVVFEEYDLPGLKTINGIAEVQGNYPSKGGLGEKGAWFRDSEGNLLAIGQPIR Sequence number 198 Sequence length: 174, Organism: Cupriavidus necator H16, PRTMKHTWILVADESLARIFASHASTAPMALVEEITDAAAHGDRANLRRDAYGRRGHAAVQGDAGHPGAHQAGPSTVTSSAGEDELHQEAQLFARRVADYLADARNKQRFDSLALIAAPRFLGLLRKALSAGVTDLITKEIGKDFTHMPNNDLQQRLADEDIIPARRDARVVTGRDR Sequence number 199 Sequence length: 102, Organism: Cupriavidus necator H16, PRT MRARFARGLTQSPVTVVARAFDGEAVRTPQVGDLVTVYSQDQDTEFNIRLCEAVEPGRWRGVVYAIGRGQDVLVTAEGLEIDDVVEVMREEIASVIPCGRSH Sequence number 200 Sequence length: 73, Organism: Cupriavidus necator H16, PRT MGSNIHVVPHDEGWDVIHEGARYAESHHATQEEAVAAGTTKAQSEHVELLIHGRDGQIRSRSSFGHDPRTIPG

[0116] Sequence number 201, Sequence length: 337, Organism: Cupriavidus necator H16, PRT MNDQTRGAVDSAELMERLLYEVKRVVVGQDHFLERVLVAILAGGHLLVEGVPGLAKTLTVKTLARTMSGSFKRIQFTPDLLPADLIGTRMYNQGTGEFSTVRGPVFANLLLADEINRAPAKVQSALLEVMEEKQVTIAGESHLVPTPFLVMATQNPIETEGTYPLPEAQVDRFLMKVLVGYPSEEEEAVIVNRVTGPSISVSPIATPERLTSLQDECGRIYVDPSLLQYAVRVVAATRKPGGYGLEDLDRYIAFGASPRATIGLIEGARALAFLRRRHYALPEDVVDLVPDVLRHRLVLSYEAMSDGVTADQLVTRILQALPVPERPLESHVRAPAQ Sequence number 202, Sequence length: 120, Organism: Cupriavidus necator H16, PRT MTVRKLPAVGFAVLMGLISIGAAAQPAQGSQQGAAPAEVPKAPPTTAQAKALIAERFKAADSNHDGKLTRDEAQAGMPKVYESFDKIDVKKQGHVTERQIGSYWMKKTKDQMQKEDPIWN Sequence number 203, Sequence length: 97, Organism: Cupriavidus necator H16, PRT MTRYIVLASFTDQGIRTVKDTTKRAAAVREMGARLGVQMKDIYWTLGEYDIVLTVEAPDDETMTSFGLTIGAAGNVRTQTLRAFNSDEMQSILGKMG Sequence number 204, Sequence length: 162, Organism: Cupriavidus necator H16, PRT MQKKPLGETPCPIARTAARVADSWSVLILREAFYGVTRFDEFQQNLAIAPNMLTRRLNSLVEEGMLERRQYCDRPPRSEYILTERGRDFRQVLLAMLAWGNKHLAPEGESVQLTNRRTGVRVEPVLVDPANGRPVTDADYHLAPGPAASDRVRQRLSRSAEA Sequence number 205, Sequence length: 236, Organism: Cupriavidus necator H16, PRTMSAAQKVVVVTGASQGIGAELVKAFRERGHRVVATARTIKPSDDQDILAVAGDIADPSTARRVVAEAVARFGRIDTLVNNAGIFVAKPFTSYTDEDYAAVTGINLTGFFHITQLAIAEMEKQRSGHVVSITSLVDHAISGVPSVLASLTKGGLNAATKSLAIEYARTGIRANAVAPGIIKSPMHAPETHEALGALHPVGHMGEMRDIVDAVLYLDNAPFVTGEILHVDGGQSAGH Sequence number 206 Sequence length: 154, Organism: Cupriavidus necator H16, PRT MSVQERLAARGLVLPQPPQPLGSYTAVSQAGDLLFISGQLPLQDGKVVWQGQVGKDLDVGQGKRAEALALNVLAQIHAYLGGFERLDHIVRVDGHISSAPGWFGQPAVLDGASDLFGEVLAEKAGHARTISSHFQQPANAAVILVVIAQIRPE Sequence number 207 Sequence length: 142, Organism: Cupriavidus necator H16, PRT MATPPNPFADFTKMMEQFRLPGVDMSAVMEARRKDIEALTEANKLAYEGIQALVQKQQEIFAQTMQQLQAAAQQYSTAGNPAEMACHSEFVQQQLHQALENMRALAETAQKAQAEALAVISKRAEQNVKEAGELLKPKSKG Sequence number 208 Sequence length: 273, Organism: Cupriavidus necator H16, PRT MTTITTQDGTQIFYKDWGSGKPVVFSHGWPLNADAWDAQMLFLVQQGFRVIAHDRRGHGRSDQPAQGNDMDTYADDLAALLDALDLRDATLVGHSTGGEVAHYIGRHGTGRVAKAVLIGAVPPIMLKTAANPGGLPIDVFDGIRKGVADNRSQFYQDLATPFFGFNRPGAKVSQGTIDAFWAQGMAGGIVGQYACIREFSEVDYTEDLKKFDVPTLILHGDDDQIVPIDAAAKRSVELVKGATLKVYPGASHGMCVVNADQVNADLLAFLKA Sequence number 209Sequence length: 344, Organism: Cupriavidus necator H16, PRT MQPSFTSTLRTAAALLALAASGTALAQKTTLTVYTAWEIETLKPYAEGFAKVAPDIELKYVRDSTGVITAKALAEKGNPQADVIAGLAASSLELLKQEGMLAPYSPKGFEKLTRDYSDKATPPSWVGLDVWGATICFNTIEAKKRNLPRPETWKDLAKPVYKGTIVMPNPASSGTGFLDVTAWLQLYGEQDGWKYMDALHENIAQYTHSGSKPCKQAGSGEFPIGISFELRAHKTLASGAPIEMIFPKEGLGYDIEAAGIVKGTKKMEAAQRYMDWLASKEANQLFAKDWAIVAYPGVARKIETIPANYEQMLVKNDFSHIAKNRERVLAEWQKRYASKSEKQP Sequence number: 210 Sequence length: 147, Organism: Cupriavidus necator H16, PRT MLVRLLYASRARQAIDAALLDAILATSLERNPRHGITGVLCHGNGIFLQALEGDRQDVSQLFQAIARDPRHHDVTLLHFEETCTRDFAGWAMGQVNAARINTATLLKFSARAELDPYRTCGAASLALLKELIAGASVVSRTGERGRH

[0117] SEQ ID NO:211 Sequence length: 67, Organism: Cupriavidus necator H16, PRT MTIESYQGYIVRGFAKQLGDGSFEASGAVEKDGRIEEGSDPLGYYPSFERAAAAGIAWAKAWVDDHG SEQ ID NO:212 Sequence length: 102, Organism: Cupriavidus necator H16, PRT MAKLVVLYKKPADTAAFDSYYFSTHVPIAKKIAGLRRYEVSSGPVASPAGDSPFHLVAVLSFDSLAALQQAMASPEGAAAAADLANFAQAGVDMLIFDTKEV SEQ ID NO:213 Sequence length: 254, Organism: Cupriavidus necator H16, PRT MWLAAILALAGCTAESVVGVLSGGRDGLWRNVQQRCLGSTAAAHPDCAQVDRSQGLVLYKDAIGKSHYLVIPARTMTGIDDARAWDGAGANPWAFGWAARGIVGRSLGKAVPDDMLGLAINSRASRS QDQLHIHLDCISSAARAVVNGAAGPIGTGWTTLRLEDKPVRAIFVPASAPVLAVDPFQLVRDGTKGGAAAAPPDRGIFVTYVRERQGGAGFVVIDQPVDSAAGSNGHASDFLDRRCRLAEGLASAAG Sequence number 214 Sequence length: 440, Organism: Cupriavidus necator H16, PRTMQRRQWLKTMGGATLGLALPAIASRVYALPARADARFLLVFLRGGYDGTSALVPAGSDFYYAARPNIAIRRPPAAGADADPNAAVALAPAFAADSAARGIGDWALHPALAATMLPLWQRGQLAFVPFAGTHDLSRSHFETQDGIEAGLPGEGEDRKSVAALRGSGFLNRLAAQAMGGQAAPVAFTDGLPMVLSGPRDVPNVSLKGSGRAPFDARQAKLLAQMYRGTRFESLIDEGFELRETVARQAQPQPGMSGNDAGTMAARMQEMQAANRRSLSARGFEVEARRMAGLMRERFNLGFIDVGGWDTHVNQGGAQGQLAGLLDNLGRGLSAFAAEMGPVWDKTTVVVVLSEFGRTFRENGTRGTDHGHGTVYWVAGGTVRGGRIIGEQVAVEQATLNQNRDWPVRNEYRAVLGGVFRRLYGLDEARLAQVFPGVRGRDIGLV Sequence number 215 Sequence length: 335, Organism: Cupriavidus necator H16, PRT MTKNDDVLVTGASGFLGSAVVRRALARGFRVRVLVRPQSPRANLAGLPVSVAEGDMRDAGAVAAALQGVRYLFHVAADYRLWADPPEEIVRTNVDGTLAVMEAAQQAGVERVVYTSSVATLRVAGARAPVDETAALRPHEAIGAYKRSKVLAERVVEKLVAERGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVDTGLNLAHVDDVAEGHFLALERGQAGERYILGGQDVMLQQMLRDIAQLCGRRPPTMQLPRWPLYPLAYGAEAAARFTGKEPFITVDGLNMSRYRMFFTSDKARKVLGYQPRPYQEGLRDALEWFRAQGYLDKR Sequence number 216 Sequence length: 218, Organism: Cupriavidus necator H16, PRTMAIRLGEQAPDFTADTTEGKISFHEWIGDNWAILFSHPKDFTPVCTTELGYMARLKPEFDKRNTKIIGLSIDPVGDHQRWVKDIEETQNCTINYPMIGDADLKVAKLYDMIHPEASGSGPRTAVDNATIRSVFIIGPDKKVKAMLVYPMSAGRNFDEVLRLLDSLQLNAKHTVATPVNWKPGEDVIIPTSVSDEEAKKKYPQGFKTLKPYLRTVEQPK Sequence number 217, Sequence length: 86, Organism: Cupriavidus necator H16, PRT MQVRIATPLFSYTGQREYVEARGASIAELLDDLDRQFPGMRFRIVDEQGKLRPYIRVFVNRTQLMRLDAPLKETDEVHILQALAGG Sequence number 218, Sequence length: 385, Organism: Cupriavidus necator H16, PRT MTTSPTPETDTGPVRLLVATTKGAWFLTSDAARRSWQIEGPTFLGHTIHHIVQDPREPSRMLMAARTGHLGPTVFRSTDGGRNWTEAARPPAFDKAPEGETGRVVDHVFWLTPGHASEPGTWYAGTSPQGLFRSADHGVTWEPVSGFNDHPNWRTWTGGEQDGTPDGPKMHSVLVDPRDARHLYIGMSSGGVFESTDGGADWKPLNRGSLATFLPDPNPEYGQDPHCMLQHPADPDILYQQNHCGIYRMDRREGVWKRIGEAMPAEVGDIGFPIVAHCRDPRTVWVFPMDGSDVWPRVSPGGRPAAYVTRDAGETWQRQDRGLPAEQGWFTVKRQAMATDGHAPVGVYFGTTGGEIWASADEGEGWQCIARNLPQVYAVTVARPA Sequence number 219, Sequence length: 250, Organism: Cupriavidus necator H16, PRTMGLCGCRQAPDAGMLVGLRARRVAWPRRHRVASLPDLPPSCRQRPLLAQSFYLPAAMRHDRSSAVPRLLALLPMLAAAVALSGCVAQYRVPAGAPASVRLLTSTDDNTSFTVVDPAKCPVPARPLVLAGTGRQLSAMGRDRSLDMAGRSPEPPARTRERLVEAGRRIYVAVTSAAAAPPLPELRCAAGVSFVPVAGGQYEIRYQRDETASQCSAQVLRMQPLAGGGARLTAEPTQQGFRALRKEYVCQAQ Sequence number 220 Sequence length: 344, Organism: Cupriavidus necator H16, PRT MTMTQQEPIQAGPLPRDLLHALRDGGYDVSGLAPAPESDTDSDPSDLRPAMPAAGPQQACHVLCAVYRATGDPTIGLWLGSRMQTDLLGLAGLTAMAGPSLGTALRRIARYQKLLAGDRIELRRQGDEAWVCIRPSDPEAPDTRPRIDMELCSLLAFGRQFTRKPLHPLRVSLRIGQPDWHLRYTEAFDCPVRFGEPEDAIVFGRRDLARLLAARVRSGPAGAGHAAREARPASASLDDVRAALQALAGDRALSLAAVARHLDISERTLQRRLMAAGTSFRTLCEEARRELAGGYLAGGAMSLGEIAFRLGFDDANSFFRAFRRWTGMTPGDYRRRAAAPPPA

[0118] SEQ ID NO:221 Sequence length: 268, Organism: Cupriavidus necator H16, PRT MTQHPPQSAMPDAPLSSPAGAVRSVQEVSALIEQSAHDLRSSLNAIQSWAYVLDRAFDTMPAPAQRALDGIRFGMQQQLALIEEMEEAVRLLADESAPRWQRLDLLALTAQAIADRRPTAEARGVLLSPVATDGAADSSEAAYLIDGDAERLAPLLRHLLVHCIWRAPAGGSVGVQVLSEPDHVKLRITESPPLDQQRSASRLTALTDFFGRRAAPDGATPARQSSALLLTRRMVEMHGAALSAESDGCDNDRIRVCIAVRFPRHMSA SEQ ID NO:222 Sequence length: 209, Organism: Cupriavidus necator H16, PRT MTGSTQDPDARRMAPATERNREPILAVLRKVLPASGTVLEIASGTGQHAVHFAAALPGLTWQPSDAEAAARASIAAWTAHAGLANVRAPLALDVCRQPWGIDAADAVVCINMIHIAPWAAAEALFAGAGKLLGPGGVLFLYGPYRRGGAHTAPSNAAFDAQLRATDADWGVRDMEAVIALGEAQGMRCDEPVPMPANNFSLTFHLNKIK SEQ ID NO:223 Sequence length: 68, Organism: Cupriavidus necator H16, PRT MPDSRPSRRLLPNCEEIHHLTMKGLDRPLSWGERLRMRSHLAICDACTSFSAQMRTLREAMHRLGRDE SEQ ID NO:224 Sequence length: 126, Organism: Cupriavidus necator H16, PRT MTDKPVRIPGPDHPITITPTAGRVVVRVAGIAIADSDAALTLQEATYPPVQYIPREHVDLAQLERTAHTTYCPYKGDCSYFSIPAGGERARNAVWSYETPYPAVAAIASHLAFYPDRVDSIEILGA SEQ ID NO: 225 Sequence length: 225, Organism: Cupriavidus necator H16, PRTMKILMVLTSHDQLGNTGKKTGFWLEEFAAPYYVFKDAGADITLASPKGGQPPIDPKSDDPGAQTDATRRFQGDPAARAALASTLTLDQVKAEDFDAIFYPGGHGPLWDLAEDKRSAALIEQFDRAGKPVGAVCHAPAVFRHTRGADGEPLVKGKEVTGFTNSEEEAVQLTKVVPFLVEDMLKANGGRFTRGEDWASHVVIAGRLVTGQNPASSDAAAQALLKMLK Sequence number 226 Sequence length: 184, Organism: Cupriavidus necator H16, PRT MRLKLSLLIAAACVAGASSALAGYNIWTGEYSLSRQELQTALDQRFPATLRYAEVVSVQLSHPRLVLDEANNRVTTHVDARLTNALLPSPPVDGKLALNSGVRYDPAKRAVLLDNPTVQQVEVAGMGQYREQLNAIGAVVAQQLLKDYPVYTFKPEELRVGGKDVEPGAITVGKDEVRVEVKQR Sequence number 227 Sequence length: 108, Organism: Cupriavidus necator H16, PRT MTALVEWAREHTCRLFPMSAEYHRSDGNQVQVSWQAEPAGSPGRCRLSAALQFDQSVIDAVYLADAAELARIGARLASVVSRRLAEDDIACLAGTALVIPVGEGLLSH Sequence number 228 Sequence length: 274, Organism: Cupriavidus necator H16, PRT MHSRTSLFPFSLAARSAAALLVAVMLGACTTAPAPDLPSAGTNLNQTQPSGPSRWEVRWQQPDGAQRDIPHGDNGQPIIFEFNEGNEGIDAAQGTVSGTSGCNRFTGSYGKTDTGIRFDRIAGTRMACPPPRMELESALLKAMQTPFATVGTQPSAGSTGRQIIWKTAGGDLLQFVEREGVGRRGASVEAGGVEKVVYIDSQRVECSGVGKMTCYRWRESPEAPWQLWYGPIEGLDFEPGVAYTLRVREYQVPNPPADASSIRWQLLKVESRTRAK Sequence number 229 Sequence length: 525, Organism: Cupriavidusnecator H16、PRT MSASQHDDQASRPLLTLQPGQVTLADLRIYRGEVRLAMAESAWAGVRAAQATVQDIIDAVVYGINTGFGKLAQTRIPNDKLAQLQRNLVLSHSVGTGPDLAEDTVRLILAIKAVSLARGHSGVRPELIEALLALANHGVTPCIPAGGSVGASGDLAPLAHMSCTLIGVGDVLVDGKRVPAAEGLAHAGLQAFELGPKEGLALLNGTQVSTALALAGLFAAEDAFAAGLVAGALSLEAIKGSVKPDFARIHEARGQAGQI AVAGAVRAMLDGSEIVDSHKTCGRVQDPYSIRCQPQVMGACLDNLQHAARILQIESNAASDNPLVFSAQGDVVSGGNFHAEPVAFAADIIALAIAEIGAISERRLALLLDTGLSGLPPFLVRDGGLNSGFMIAQVTAAALASENKALAHPSSVDSLPTSANQEDHVSMATYGARLRGEMAANTAVVVGIEAMAAAQGIEFHRPLKSSPLVEQELARIRARVAFVEEDRYLAPDIEAMKVVQWQGDAGAGWPDAVRAILPTNLS Sequence number 230 Sequence length: 338, Organism: Cupriavidus necator H16, PRT MCTTCGCGDTTGAVVSGPAAEGEPAPTAGAGQDVVHAHPYPDAMSPGPAQEAGGMVLRKHPADDHAGHAGALLHAPMHRRAMHYHDHPLEHAHAHSHEHAHEHAHEHAHIHGTSVVIEQDILAKNQWIAQRNRGWLAGRSIVALNLVSSPGAGKTTLLERTIRDLGPTLELTVIEGDQATLNDAQRIRAAGCRAIQVNTGTGCHLDADMISRALTALDPPPHSVVMIENVGNLICPALFDLGEQAKVVILSVTEGEDKPLKYPHMFRASSLMLMTKTDLLPYVSFDVARCLACARQVNPDIAILQVSALSGAGMDDWYLWLRTACAGRIAPPEDSFPA

[0119] Sequence number 231 Sequence length: 355, Organism: Cupriavidus necator H16, PRT MNDRVPVPPARAARVRDTTINLTHGSGGRAMRDLIEDVFVSTFDNPALAALEDQAVFPLVSLAAHGDRLAFTTDSYVVDPLFFPGGDIGTLAAAGTVNDLAVCGATPLFLSCGVVLEEGLPVDTLRRVAASMQRVARAAGVAIVTGDTKVVERGSADKLFINTAGIGVVRAGLSISARHARAGDVVLVNGYLGDHGAAILVARQQLALEADLQSDCRPLNGLVAAMLDACPDIHCLRDATRGGVATVLNEFAQASGVAIRIREVDVPLREAVKGACEILGLDPLYLANEGILVAIVPPEDAARVLAAMRAHPDGRQAAAIGEVEPGPAGLVVLHTGFGGQRVVDMLVGEQLPRIC Sequence number 232 Sequence length: 169, Organism: Cupriavidus necator H16, PRT MMPFDIRVGQGYDVHALVPGRKLILGGVEIPHDRGLLGHSDADALLHAVTDALFGAALGDIGRHFPDTDAQFAGADSRVLLREAARRVREAGYEIGNVDATVIAQAPKLAPHIGAMVANLAEDLGIARGRCNVKAKTNEKLGFEGRQEGIVAQAAVLLWRASVGDAQD Sequence number 233 Sequence length: 257, Organism: Cupriavidus necator H16, PRT MSALRSCRRGFLLAACVATLALAAPPAFAADLVVSAASSLTNAFKSLAESFERAHPGTHVVLNFGASDVLMQQIIKGAPADVFASADQEAMNKAEAEKVIAPASRRNFAANQVVLIVPADSKLAIASLQDLTRPEVKRIAYGNPASVPVGRYTRGALEAAGLWDAVAAKGVPAQNVRQSLDYVARGEVQAGFVFATDAAVMPDKVKVAVRVPSRTPVTYPIAITGQTRQQKEAAQFVDYVASPEGQAILSRFGFQKP Sequence number 234 Sequence length: 127, Organism: Cupriavidus necatorH16, PRT MSIPSRHREALQQQAEGRVTLTKHPDGCLLLFPRPEWETFRTRIAALPMDAHWWKRIFLGNAADVEMDGAGRVLIAPELRSAAMLDKEVMLLGMGSHFEVWDAATYAAKEQQAMAQGMPEALKNFSF Sequence number: 235, Sequence length: 109, Organism: Cupriavidus necator H16, PRT MPTAKRAIPIQPLPCGEEWHIAGIVVHTLPDSLPQVRAAIEAITGAEIHAVSDDGKLVVTVEAPTSRAIAAHLTCLHQLEGVLSAALVYQHNEDAAAMNEEMADEDLSS Sequence number: 236, Sequence length: 106, Organism: Cupriavidus necator H16, PRT MQKILAVCAIAAASILVSGTAQARVDVGIGIGIPGVVIAAPPPPVYYEPAPVYVAPRPVIVAPPPGYYDDDWQARAWREEQWREQRWREREWRERHRRWHRWHDDD Sequence number: 237, Sequence length: 114, Organism: Cupriavidus necator H16, PRT MICGLRFRYDQIEPPMTPLSPQARATLLADLPGWTTVPDRDAIFKRFTFHDFNAAFGFMTRVAIQAEKADHHPEWFNVYNRVDITLSTHDANGLTQRDIDLAHFIERAADAVTD Sequence number: 238, Sequence length: 305, Organism: Cupriavidus necator H16, PRT MTSIRVLGSAAGGGFPQWNCNCHNCDGVRRGTVRATPRTQSSIALTGDGPDAILVNASPDILQQLRQAPALQPARAPRDTAIAAVVLMDAQIDHVTGLLMLREHREALPLYATAAVLDDLGAAFPLTRILSHYCGLRTHALPCDGTAFSVPPLDGLTLTAIPLESKAPPYSPNRHAPRAGDNIGLRIEDRRSGRSAFYAPGLGRIDDHVFAELHSADIVLVDGTFWRDDEMQAPGFSGKSAADMGHLALSGPGGMIEVLERLPASRKILIHINNTNPVLVEDSPERAELARHGIEVAYDGMEIALAccession number 239, Length: 172, Organism: Cupriavidus necator H16, PRT MYQGLAAARDDPRLPVRPAGAYTERRNRHRPGRFALPPSLPAMQHLSPADAQDLLAQSPETLFIDCRSEMEYLFVGHPKGAHNVPWNDGPDWEVNPHFVQMVKKLAGQASARPVLLICRSGNRSSAAARALEGAGFSNVQFVLHGFEGDLDAQRHRNTVNGWRHDGLPWEQY Accession number 240, Length: 299, Organism: Cupriavidus necator H16, PRT MVAFHRRNKRSSSGNVTHLRPFRHNQLRGADDNFDDEAADDHDIVYQRPRRRGIYLLPNAFTTAALFAGFFAIVQAMNMRFDAAAIAIFAAMVLDGMDGRVARITNTQSAFGEQYDSLSDMTSFGVAPALVMYEWILHDLGKWGWIAAFVYCTCAALRLARFNANIGVVDKRFFQGLPSPAAAALVAGFVWLVIDNKLPVKELWMPWVAFGITLYAGLSMVSNAPFYSGKALDVRYRVPFGMMVLVLVLFVVVSTDPPVALFGLFVAYAISGYVLWAWRAMHGKPAIEKKQRENGGKAS

[0120] Accession number 241, Sequence length: 299, Organism: Cupriavidus necator H16, PRT MQIDLNADLGEGCANDEALLALISSANIACGWHAGDAATMVQTVKWALERGVAIGAHPSYPDRENFGRTEMQRDPEHVYADVLYQIGALDAIVRAQGGELHHVKPHGALYNQAVRDPALARAIVRAVRDFDADLVFFGLAGSQMIDIAREAGLRVKQEVFADRGYNPDGTLVKRGSPGALHEDEEVALNQTLTMVREKRVRAIDGTWVPIQAETVCLHGDGAHALAFARRIRERLGAEGIAVRAGD Accession number 242, Sequence length: 197, Organism: Cupriavidus necator H16, PRT MSLDPAELQAIRPYLLRFARLQLRDQALAEDAVSETLLAALEHPERFAGQSTLRTYLVGILKHKIIDTLRSGKREVRLALATDAEGQRQSDDDAFDALFTRNGHYQDPPSDWGNPERAFERREFFEILQLCVDRLPERTGRIFMMREWLELDTEEICQHLQISATNAWAMLYRARMRLRECLELHWFGQRGRPGKAG Accession number 243, Sequence length: 333, Organism: Cupriavidus necator H16, PRT MSPTGTPATPALRHRTVLLDEAVDALVWRPDGVYVDGTFGRGGHSRAVLARLGPDGALVAFDKDPAAIAEAGTIKDARFSIEHASFAAMAERLSGRGHVAGVLLDLGISSPQIDEAARGFSFRFEGPLDMRMDTTRGITAAQWLAQADEQDIARVIRDYGEERFAVQIAKAIVARRSEPGDGGPIATTADLAALVAKAVKTREKGQDPATRTFQALRIHVNQELEDLERGLKAAYELLQVGGRLVVISFHSLEDRIVKRFMAAHARPQQDADPALRRAPLRAADLPQPTLRLLGRYKPGPEEVAANPRARSAVMRVAEKLAPAATTAGGGARA Accession number 244, Sequence length: 517, Organism: Cupriavidus necator H16, PRTMAYLSTGSEWTFELICRYDREIARIAGEFGLDTYPNQIEIITAEQMLDAYASAGLPVGYSHWSYGKHFLASERSYQRGHMGLAYEIVINSNPCIAYLMEENTMPMQALTIAHACYGHNSFFKGNYLFRTWTNADAIVDYLLFAKNYVAECEQRYGEREVELLLDSCHALQNYGVDRYKRPKKLSITEEKARQADRENYLQMQVNDLWRTLPKHRPHALASEDEASEGEIFPPEPQENLLYFIEKNAPKLASWQREIV RIVRKIAQYFYPQRQTKVMNEGWATFWHYTIINQLYEEKLVNDAFMMELLQAHTNVIYQPPYNSPYYSGMNPYTVGFLMFQDLRRMCENPTEEDYRWAPEFAGSDWRKTLDFAMRNFKDESFLLQFLSP RVIRELKLFSVLDDDREGKLRVTAIHNDEGYRAIRQLMAAQYDLSSMEPNIQVTNVDVGGDRSLTLRHLQNDRRPLAHNFDEVIRHVTRLWGFTVRLEVAYEDGRTELKYECKPERRHRRPHGGGLSLAA sequence no. 245 Sequence length: 192, Organism: Cupriavidus necator H16, PRT MAKKILMLVGDYAEDYETMVPFQALQMVGHTVHAACPDKQAGDACATAIHDFEGDQTYTEKRGHNFTLNASFAEIDPAGYDALVIPGGRAPEYLRLNARVLEIVRHFAQADKPIAAVCHGAQLLAAAGVLEGKTCSAYPACAPEVKLAGGTYADIPVDQAHTDGNLVTAPAPAPAHPAWLAQFLAVLGTRIVH Sequence number 246 Sequence length: 192, Organism: Cupriavidus necator H16, PRTMLKIDKLLSAPHGIAPVLVRRAPKLVLPFAERSKSRLRAVLDNGDEAALFLARGTVLRGGDLLVAEDGSFVEVQAAAEAVLEVRAEDPHALMRAAYHLGNRHTPVEIGRDYLRLEYDAVLADMLQRLGVRAERAELPFEPEAGAYGGGHKHGHDATFAEDYAAAQAVFHEHHGHSHSHSHDHVHDEKCGHKH Sequence number 247 Sequence length: 209, Organism: Cupriavidus necator H16, PRT MTQARTKKNPPLRVGVGGPVGSGKTTLLEMLCKAMRDRYDLVAITNDIYTKEDQRLLTVSGALPAERIMGVETGGCPHTAIREDASINLEAVDRMLARFPDADVVFIESGGDNLAATFSPELSDLTIYVIDVAGGEKIPRKGGPGITKSDLVINKTDLAPYVGASLEVMESDARKMRGARPFVMGSVKSGQGLDEVIRFIERQGMLGV Sequence number 248 Sequence length: 822, Organism: Cupriavidus necator H16, PRTMNKQTEPFLLDATAEQVSQVGISRPHESAHLHVAGTATYTDDIPELAGTLHAALGMSTRAHARIKSISLDKVRAAPGVVDVLTVDPIGTNDCGPIIHDDPILARDVVQFIGQPVFIVVATSHDAARRAARLGTIDYEDLPPVLSPQAAHEAGSYVLPPMHLTRGEPAARIASAAHQDSGKIHLGGQEQFYLEGQISYAAPREND GMQVWCSTQHPTEMQHAVCHMLGWQAHQVLVECRRMGGGFGGKESQSALFACCAALAAWKLMCPVKLRPDRDDDMMITGKRHDFVFDYTVGHDDEGHIEGVKVEMVSRAGFSADLSGPVMTRAICHFDNAYWLPNVQIDGYCGKTNTQSNTAFRGFGPQGAFAVEYILDNVARTVGKDSLDVRRANFYGKTEHNVTPYGQTVEDN VIHELIDELVASSEYRARREATRAFNATSPVLKKGIAITPVKFGISFNVAHFNQAGALVHVYNDGSVLVNHGGTEMGQGLNTKVAMVVAHELGIRMERVRVTATDTSKVANTSATAASTGADLNGKAAQDAARQIRERLAVFAARKAGVEPSEVRFNDDLVSAGELRVSFGELAREAYVARVQLWSDGFYTTPKLHWDQSKLQGRPFYYFAYGAACSEVLVDTLTGEWKLLRADALHDAGRSLNPAIDIGQVEGAFIQGMGWLTTEELWWNKDGKLMTHAPSTYKIPTVNDCPEEFNVRLFQNRNVEDSIHRSKAVGEPPLLLPFSVFFAIRDAVAAVGDYRINPPLKAPATSEGDPGRRRRRARSRGAASLSWRNDRRKRAPAGAPGLSPLRVDGHGAGGRSRRAPPLR Sequence number 249 Sequence length: 246, Organism: Cupriavidus necator H16, PRTMKLQGRVAIITGAAAGIGFATAQRFAADGAIVVLCDVQEARVREAADKLAATGATVSAYRVDVTRRDEVDAMVAAVLAAHERVDILVNNAGITKDARLGKMTEAQFDAVIDVNLKGVFNCAQAVAGLMSEQGKGVILNASSVVGLYGNFGQTNYAASKFGVIGFTKTWARELGPKGVRVNAVCPGFVNTEILQTVPEKVLDGMKSSCWLRRLAEPAEIASIYAFLASDDASYVNGVAIEASGGMSL Sequence number 250 Sequence length: 195, Organism: Cupriavidus necator H16, PRT MSQIDNAALAQLFTEARTHNVWQDRHVDDAVLHQIYEAMKFGPTAANSSPARIVFVKSAAAEKARLVDCVSAGNVDKTRSAPVTAIIAFDTAFHDQLPKLFPHADARSWYAGNDEKIARDALMNSSLQGGYFILAARALGLDCGPMGGFDADKVNAAFFPDGKWQVNFLCNLGYGEADKLFPRGPRLSFDEACRIV

[0121] Accession number 251, Length: 500, Organism: Cupriavidus necator H16, PRT MTLPNLSATVAQPGLAGNGARADGQQRRATLDHLSLSPGRDAQALGQRSRNAVWHPCTRLRPDDDAVPLAIVRGVGPWLHDADGRRYFDATSSWWVNLFGHANPRINAALASQLETLEHVMLAGCTHAPAVELAERLSALTGGALGNVFYASDGASAVEIALKMSFHYWRNTGLPAKREFVCLRHGYHGETVGALAVTDVEVFRDAYDPLIRRAHVVMSPDARQAAPGESAADVAARALAELETLLRERAGQIAALIVEPLVQGAAGMAMYDPSYLDGARALCDRYRIHLIADEIAVGCGRTGTFFAWEQSRAGEAPLPAHLWPDFLCLSKGISGGYLPLSLVLSRPEIQRAFVADELARSFLHSHSYTGNPLACRAALATLDLFAQDDVLARNRERAHWLADGMAALAADARLRHVRQRGLIWAADVRDDVAGADFAARFHHAARERELLVRPIGNTLYVMPPYVFDAAQARWLGEQMMATLDDVTTDVTTSGEVRHAA Accession number 252, Length: 343, Organism: Cupriavidus necator H16, PRT MTQAHTATTVTTVSVDSLRQSARSHALPDDAAWRVDDVAALFALPFNDLLFRAQQVHREHFDANTVQLSTLLSIKTGGCEEDCGYCPQSAHHDAGVKAEKLMALDEVLDAARAAKANGATRFCMGAAWRSPKDRHLEPVMDMVREVKAMGLETCVTLGMLKAEQAQQLKEAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVRDAGINVCCGGIVGMGEAREARAGLIAQLANMDPYPESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPRAMVRLSAGREAMDEALQALCFMAGANSIFYGEKLLTTGNPQADRDRALLARLDIRAEGYAG Accession number 253, Length: 369, Organism: Cupriavidusnecator H16, PRT MSIQVKNVEKRFGDFVALDNVSLDFDEGELTALGPSGCKGKTTLLRIIAGLERADAGQIVLAGRDASDQHVRQRQVGFVFQHYALFKHMSVFENVAFGLRVKPRAERPSEAQIRDKVRSLLELVQLDWLADRYPSQLSGGQRQRIALARALAVEPRVLLLDEPFGALDAKVRKELRRWLRRLHDELHVTSVFVTHDQEEALEVADQVVLMNRGHVEQYGSPEAVYNHPATPFVFGFLGNVNLFHGRLEVGESGGLLHTGEAVLPVVGSGHETAGDAVAYVRPHDLDLERYSPGADGIAVTLRRALTLGPVAQLELEREDNQDVIEVALPLERFRHAGFREGELLAVRPRQLRVFTQPRTTSTQQEKATS Sequence number 254 Sequence length: 560, Organism: Cupriavidus necator H16, PRTMYQYDQYDQRIVAERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPYGLLSSKQMRKLGHIAREYDRGYGHFSTRQNIQYNWMELERVPDVLAELASVEMHAIQTSGNCVRNITTDHFAGVAPDESV DPRVLAELLRQWSTFQPEFAFLPRKFKIISASANDRAVVQMHDIGIYAYKNAEGETRLRIGAGGGLGRTPILGTIIKDDLPWQHMLSYVESAIRVYNRYGRRDNKYKARIKILVKAIGAEKFAQEVEEEWQHSKDGPAT LTQAEFDRVAQYFTPPAYEKLADTDASYENHLLEDKAFARWVSRNVHGHKVPGYAAVTLSTKPGVASPPGDATDAQIEAVADLADQFGFGELRVAHEQNLILPDVKKQDLYALWQLARKAGLATPNIGLLTTDIIACPGGDFCSLANAKSIPIAQAIQDRFDDLDYVYDLGEISLNISGCINACGHHHVGNIGVLGVDKDGSEWYQVTLGGAQGNQTALGKVIGPSFSAEEMPEVVTRIIDTFVANRIEDERFIETLSRIGIAPFKERVYADKQPRERAEA Sequence number 255 Sequence length: 105, Organism: Cupriavidus necator H16, PRT MESSEVLQEIREVNLAYLLLAQRLVRENQVEAMFRLGVSKEIADILAKLTSAQLVKLAASNMVLCRFRFDDHALLSTLTHTAKSHDMQQIHAAILLARQPVESLN Sequence number 256 Sequence length: 435, Organism: Cupriavidus necator H16, PRTMAQVINTNSLSMTQNNMNTSQSSLNTAIQRLSSGLRINSAKDDAAGQAIANRFTSNIRGLTQAQRNANDGISLAQTTEGALTEVNNNLQRIRELSVQAATGSNSSSDLKSIQDEINQRLSEIDRTSQQTDFNGVKVLAGSNKLSVQVGANDGETISIDLKQIDRTTLGLSGFSVAKNSLDVGPKITTINAAAGTGSFNVSFAAGDVTKINAATGKTFAAADLELHEVKTAAGANSGQFVVKAGDDYFAASVDRATGAVKLNEADVAFDDTANGIAGPVTLQDQVVRVANDAAGVATGYVTVQGKNYVAAGTLADGGAAGTLNVAVGTISLSGATPTAEFTGVPTGNALKKIDAALKQVDDLRSSLGAVQNRFDSVISNLGTTVTNLSSSRSRIQDADYATEVSNMTRANILQQAGTSVLAQANQTTQGVLSLLR Sequence number: 257 Sequence length: 267, Organism: Cupriavidus necator H16, PRT MNDINPAFVMPDVQSSPDTRQIPIQRVGVKGVRYPVTLKTPAGIMPSVGTFNLDVHLPADQKGTHMSRFVALLEEERAPLELSSFRLMLDKMLEKLEAEAGRIEVTFPYFISKTAPVSGVESLLDYEVTLTGEVRDGAARVFLKALVPVTSLCPCSKKISQYGAHNQRSHITMNVELAGELPVEALVRMAEEEASCELWGLLKRPDEKFVTERAYENPKFVEDLVRDIAMRLNADDRIVAYVLEAENFESIHNHSAYAVIERDKRVD Sequence number 258 Sequence length: 231, Organism: Cupriavidus necator H16, PRTMPLSQPVNRSQRHRRAITAEAYQREDGLWDIEVRLTDTKPRDIELAGDRVRPVGQPLHDLWLRVTINEEMTVLDAEACSDWVPYPSHCDTIGPAYRKLIGLNLRKGFRKAVRERLGGMHGCSHLTEAAGVLPSTAIQAFAGEVINIRDNGEDDPNPEPPYQLNGCHALRFDGEVVRQFYPRWYGHQPTPKVRAEAAPAVATAPQAPPAANGDRGNDGNDAVPAERPTGTSG Accession number 259, Sequence length: 403, Organism: Cupriavidus necator H16, PRT MSKRILVIGAGFAGMWSALASARLLDQVGRTDVEIALVAPKPELHIRPRLYEQNPNGMKAPLQDIFQAVGVRFIQGKVEHIDVANQTVSVAGVGADTPRQRISYDRLVLAAGSRLFRPQIPGLDQHAFNVDQVADAAELEAHIHGLADRRESAGRNTVVIAGAGFTGIETAAEMPARLREVLGEDAAVSVIMVERNAAIGPDLGEGPRPVITQALAELGVTWKLGSGVAAVDAKGVMLENGERIEADTVIWTAGARANELTAQIPAERDNFGRLHVDRNLKVKGVEAVFATGDCAYAATDDEGNFATMSCQHAMNLGRSAGHNVAADLVGAAPIPYSQPKYVTCLDLGPWGAVYTEGWNRQVKMTGGVAKALKTRINTEWIYPPSANREEALALADPRFIVVA Accession number 260, Sequence length: 174, Organism: Cupriavidus necator H16, PRT MKRSSALCLLAAATLAVPVQALAYWQWRDANGRMVYSDVPPSAAAARVLRAPGRFAGELQPVEAANAGAPAPAPAPAPAKASAKPAAAPAPTPEEAFQKRREASLKAATEEARKESEAAEREARCAQLRNYANGLQQGMRAAVAGPDGSLQHLNSDQRQAEVLKTNASLEKNCS

[0122] SEQ ID NO:261 Sequence length: 308, Organism: Cupriavidus necator H16, PRT MRALPPTGLAVGGAGAPDASGAPDSAQLRLFQEDMFEGIARLPDGSVDLIVADPPYGLGKDYGNDSDLLSGQEYLDWSERWMNAVVPKLAPKGTLYLFCTWQYSPELFVMLKRRLTMINEIIWDRRVPSMGGTTRKFSSVHDNIGFFARARDYYFDLDPVRIPYDPETKKARSRPRFEGKKWLEVGYNPKDLWSVSRIHRQDPERANHPTQKPLEIVERMVLSSCPPGGLVLDPFAGSGTTAVACLRHGRRFAGFEINPEYVQVACDRVSAAAAALAVTNAAAEPAATPTLAAANDDVSPAPRPHLIP SEQ ID NO:262 Sequence length: 193, Organism: Cupriavidus necator H16, PRT MAKIIQLQQDGATVAPVIVDDGWTVLRGTAEQPLTDEQIAAAVQSQDAVLFPLSVFKANEALLAGRDAARTGVWLAPEDEPGDAEAYFARVALVAVDFPVFRDGRGFSTAYLLRTRYHWKGQLRAIGDVLRDQLNFMKRCGFDAFAVRADKNIEDAIKGFTEFTVTYQASVDEPLPLFRRARAEVGAQPKETA Sequence number 263 Sequence length: 163, Organism: Cupriavidus necator H16, PRT MDTLIGTDNRWPPQTAGEERGLWKSTMAAASHALGAAGRVQQAVSQTLKLQHKIRALRDELHQMEAERDVYRELHARTVEELHQAIDRSPAEIRRLRAETEAMQVRHRAYKLLVQHYMRLGTPIDPGVFAEQRSRVQQHILFQRRKGIPVAQIRVEDIAFLLR SEQ ID NO: 264 Sequence length: 279, Organism: Cupriavidus necator H16, PRTMKPSLMSAASTRIATRVAVLALAALAPLAAHAHRQWLLPSATVLSGSDPWVTVDAAVSNDLFYFEHVPMRLDNLQVTAPDGSAVKAENAATGKYRSTFDLHLTQPGTYRVAVVNNGLFASYKVDGQAKRWRGSAENFAKEVPANAQDLQATQAEGRIESFITAGKPSDKGLRTAGRGLELPITHPNDLVAGDTASFRMLLDGKPASNLKVSVVPGGIRYRDQLQEFSATTDADGKFSVKWPAPGMYWMEAEVKDDKTTFKQAKSRRAVYAVTVEVLPQ Sequence number 265 Sequence length: 189, Organism: Cupriavidus necator H16, PRT MTQWSPEQFIKVQMAGIEALTGLTGKAFEGFEKLLELNLQAMKTALADSREGARKALSAKDPQELVELQIALFQPADNVLAYRRQLYDILAATRAEFEKVAEVQYTAGKQGLHDFLGSVVSQTPSGAAAAPLAAWQEAVSATTTLYESMQTTAKQAVQAAESSFNTAAQVASKGMQRRAAQASKAAAN Sequence number 266 Sequence length: 413, Organism: Cupriavidus necator H16, PRT MQQQKRRTLIALAIVVVLGAGVAGSILRPWHGGEAHANTPPPAPAIEVAAVVGQTITEWDEFSGRVEAVERVEIRPRVGGTIDAVHFREGALVKKGDPLFTIDPRPYAAEVARADAALGAAQVRAAHAQSEQTRAQRLLDDNAISKREFDERINAARETSANVRAAQAQLETARLNLAYTRITAPVAGRVSRAEITVGNLVAAGTGSPALTTVVSVSPIYASFEIDEQSYLRYTAPGAGGGQPNLPVYLGLANEDGNPHEGKIQSVDNRLDPRSGTIRVRAVFQNEDGRLLPGLYAKVKLGGGAPHAAVLVNDRAIGTDQGKKFVLVVDKTNKLVYREVELGPNHDGLRVVRKGLKPGETIVVNGLQRVRPGDTVQPKPVAMAFRSELEPRGTTPDRKQAASEKGEADVKPVSSequence number 267 Sequence length: 245, Organism: Cupriavidus necator H16, PRT MSTRAPFACFVTGTDTGIGKTHASATLLHALHAAGYRTAGMKPVASGSEWRDGHWHNDDVAQLRAASSVAVPLGQTCPFLLRTPCSPHLAAAHEGVRITRAPVRAAFDALRRQADAVVVEGVGGFNVPLDAGAVRWNTADLAVMLAVPVVMVVGIRLGCLNHAVLTAEAIRARGLPLAGWIANRVDPDMLLADENIATLHASLDAPCLGELPWQLAPAAAAGRLDLLAPLFAAATQYQAEAAGLAA Sequence number 268 Sequence length: 404, Organism: Cupriavidus necator H16, PRT MLLEQLRRAAEQRHALALTRRRRVAHTACAPHQAVGEDGSEPESLLTFCSNDYMGLANHPDVIAALVEGAQRYGAGSGASHLVSGHSLAHTQLEAELARWLAPHIPHACTLYFCTGYMANMAVLTALGTAGATLFCESLNHASLIDGARLARADVQRYPHCDTAALEALLAASTSERKLIVTDSVFSMDGNVAPLRKLLELAERHDAWIIVDDAHGFGVLGEQGHGVLEALGLSSERLIYIGTLGKAAGVAGAFVAAHETIIEHLVNTARPYIYTTAAPPAVAHALLASLAIIEGEEGTQRRAQLTRCIGMLREGLAQLAAIAGWTLGDSETAIQPLIVGDNGAALASSATLEADGIRVGAIRPPTVPEGTARLRITLSAAHTEDDVRRLLDALSAVAQREVA Sequence number 269 Sequence length: 757, Organism: Cupriavidus necator H16, PRTMANRREVPGIRKYDGPAGGWGALKATATAIRTQMDTVKAPITLMRTNQPDGFDCPGCAWPDKEHRSTFQFCENGAKAVTWEATTKRVPPEFFATNTVSSLLNRTDYELEDFGRLTHPLVYDRATDTFRAVEWEAAFARIGEILRGLSPDQVEFYTSGRASNEAAYLYQLFAREYGTNNFPDCSNmchePTSVGLPRSIGIGKGTVSLEDFDTCELIISIIGHNPGTNHPRMMGTLHEASRRKVPILVFNPLRELERALERFADPQNMVEMATYGSTRIASSYFLVRAGGDAAAAIKGVMKALLDLEASQGNVLDHDFIAVHTEGFEALADDLRSTEWADIEKTSGLTRADLELVAAAYAKSNATIVTYGMGVTQHNEGTANV RLLCDLLMLRGNFGKPGAGICPLRGHSNVQGNRTVGITEKPSAGFLQRIEDTFGFSPPAGHGHDAVNAMQAMIDGKSRALFCLGGNFAVALPDPEQCFPAMGKLDLSVHVGTKLNRTHLLVARETYVLPCLGRTELDVQETGPQSVTVEDSMSMVHASAGKLAPASEFLRSEPAIVAGIATATLPDSKVPWLELVADYDRIRDLIEKTVPGFEDFNAVRVRVPGGFRMPLPPTERKWETSSGKAEFYVFKGLHEDKQVHADDVLRLVTIRSHDQYNTTIYAMDDRYRGVFGRRDVLFMNPADLAARGLEHGDLVDIETIAAGRQLRFEKITAIEYEIAPGSVAAYYPEANRLVPLDYIDEDSGTPSYKSVPVRVVRSEVT Sequence number 270 Sequence length: 336, Organism: Cupriavidus necator H16, PRTMTIKVAINGYGRIGRNVLRAHYEGGKRHDIEIVAINDLGNAATNAHLTQYDTVHGRFPGEVSVDGDAFRVNGDRIRVLAQRNPAELPWGELGVDVVMECTGLFTSKEKASAHLRGGAKKVIISAPGGKDVDATIVYGVNHGVLKATDTVISNASCTTNCLAPLVKPLHEKLGLVNGLMTTVHSYTNDQVLTDVYHEDLRRARSATMSMIPTKTGAAAAVGLVMPELDGRLDGFAVRVPTINVSLVDLSFVAARPTTVEEVNGILKAAAEGELKGILDYNTAPLVSVDFNHNPASSTFDATLTKVNGTLVKVSAWYDNEWGFSNRMLDTAVALAHAR

[0123] Sequence number 271 Sequence length: 670, Organism: Cupriavidus necator H16, PRT MNAPERIDSAARCANALRFLAADAVEQAKSGHPGAPMGMAEMAEVLWRRHLRHNPANPAWPDRDRFVLSNGHASMLQYALLHLTGYDLPMSQLRQFRQLHAATPGHPELGVTPGVETTTGPLGQGLANAVGMALAEKLLAATFNRPGFDIVDHHTYVFLGDGCLMEGLSHEACSLAGTLRLGKLICLYDDNGISIDGEVAGWFADDTPRFAAYGWHVIADVDGHDAHALDAALHEAKAERDRPTLICCRTVIGKGAPAKAGGHDVHGAPLGAPEIAAMRTALGWEAEPFTVPADVADAWDARAQGAAREAEWEARFVSYCAAHPELAEEFVRRA NGRLPEGFDAELMALLDAPSPLQGKIATRKASQLCLEALTPALPPELLGGSADLTGSNLTVNVKASVWVNHAGHGNYVSYGVREFGMAAAMNGIALHGGLIPYGGTFMTFSDYSRNAIRMAALMRLRVVHVLTHDSIGLGEDGPTHQPVEHAASLRLIPNNQVWRPCDGAETAYAWLAALRREDGPSCLVLSRQALMPFERNPAQRAEIARGGYVLRDVPAPRVVLIATGSEVEIAMRAALDLADAGIAARVVSMPCVELFYAQDVAYRDTVLPPGLPRVSVEAGGTWFWRGVVGEQGLALGIDTFGESAPAEALYQHFGLTPAHVAAARVLLEEA Sequence number 272 Sequence length: 670, Organism: Cupriavidus necator H16, PRTMNAPERIDPAARCANALRFLAADAVELARSGHPGAPMGMAEMAEVVWRRHLRHNPANPAWPDRDRFVLSNGHASMLQYALLHLTGYDLPMSQLRQFRQLHAVTPGHPEVDVTPGVETTTGPLGQGLANAVGMALAEKLLAATFNRPGFDIVDHHTYVFLGDGCLMEGLSHEACSLAGTLGLGKLICLYDDNGISIDGEVAGWFADDTPKRFAAYGWHVIADVDGHDAHALDAALHEAKAERDRPTLICCRTVIGKGAPAKAGGHDVHGAPLGAPEIAAMRTALGWEAEPFTVPADVADAWDARAQGAAREAEWEARFVSYCAAHPELAEEFVRRANGRLPEGFDAELMALLDAPSPLQGKIATRKASQLCLEALTPALPELLGGSADLTGSNLTNVKASVWVNHAGHGNYVSYGVREFGMAAVMNGIALHGGLIPYGGTFMTFSDYSRNAIRMAALMRLRVVHVLTHDSIGLGEDGPTHQPVEHAASLRLIPNNQVWRPCDGAETAYAWLAALQRENGPTCLVLSRQALMPFERDAAQRADIARGGYVLRDVPAPRVVLIATGSEVEIAARAALDLADAGIAARVVSMPCVELFYAQDAAYRDSVLPPGLPRISVEAGATWYWRGVVGEQGLALGIDSFGESAPAEALYQHFGLTPAHVAAAARVLLEDA SEQ ID NO: 273, Sequence length: 231, Organism: Cupriavidus necator H16, PRT MATVSMPCTAVLIDLDGTLVDSAPDIVEAANRMLADFGSPALPFDTVAGFIGRGVPNLVRRVLETAGLTPRVEAAEAVAMFHRHYAETNGRLGSVFPGVEAGLEALRRQGYRLACVTNKPRALAVPLLALTGLSQYLEVLVAGDSIAQMKPDPEPLRHACNLLDVDTAQGVLVGDSAVDVAAARAAGIPVCLVRYGYAGPGGPAALGADALLDSLEALPALLTPARLAPAA SEQ ID NO: 274, Sequence length: 565, Organism: Cupriavidusnecator H16、PRT MTRIAIRQYSPEHASSLAAAGLHPTLARILAARGVARAAELATDLPELVPPAAMKGIGHAAAYLADAIAAGKRLLIVADYDCDGATACAVGVRGLRMLGARVEYIVPNRFEYGYGLTPEIVALAARQQPDVIVTVDNGIAS VDGVAAANALGIDVVVTDHHLPGSELPQAAVIVNPNQPGCEFPSKNLAGVGVMFYVLLALRAELRQRGVYTQATQPPLQTLLDLVALGTVADVVKLDTNNRILVAQGLRRRAGRMQPGVAALFRAAGREAARANTFDLGF GLGPRLNAAGRLADMSLGIECLLTEDANRAWEIAQELDSMNRERRDIEAAGMQQEALQILEQPLAGPDPSARFTVSVFNDTWHQGVIGIVASRLKDKFHRPTITFAPGDETTVKGSGRSIPGFHLRDALDLVSKRHPGMLLKFGGHAMAAGLTVRAERFAEFQEAFEAVGREWLTDDQLARVIETDGDIEDQCFSPEFVTLLEQQVWGQGFPAPTFCGEFDVLRQSVLKGKHLKLQLGRGSQRLDAIWFNHADSLGASAQVAYRLDNNTFNGVTRVQLVIEHAQ Sequence number 275 Sequence length: 128, Organism: Cupriavidus necator H16, PRT MHANRPLIQTLLAATAALASSIAFAHPKLVSSTPADKAEVAAPQKIELQFSESLVTQFSGANLVMTGMPGMADHAPMKMAVKVSGSDDPKTMVITPTQPLPPGSYRVDWRAVSSDTHPVNGKIAFTVK Sequence number 276 Sequence length: 752, Organism: Cupriavidus necator H16, PRTMRIRGIEALAGGQAGNSGEARPNTGVATLDRRSFLKLTGLAGGGLALGVAPLVQAQEGAKPKAPPPAPQAFLIIAPDNTVTVAVNRLEFGQGVHTALPMALAEELDADWRNVRATLAPAGDPYKDPGFGMQMTGGSTALNHSFQQYRELGARARAMLVAAAAQQWKVDPASCKVEQGVVTSGSHRATFGELAPAAMELPVPQRVTLKDPSQFRIVGKPTPRLDARGKMDATTPFGIDTHLKGMVVAVVARPPRFGGKVKSFSADKARAVQGVRGVLQVPVDRGGSGVAVIASGYWPAKMGRDALEIQWEDAGSKVSSQALFDEYARLAGQPGTVARAGSGDIGSAINGAARKIEADFRFPYLAHAPMEPLNCTLQPEVAGGKVQSVKVWVGSQFQTVDQAAVARTLGLAPDKVVLNTMMAGGGFGRRAVPTSDYIVEAANVLKAWVAAGHTEPLKVVWSREDDIRGGYYRPLHLHRARIGLDAQGKVVGWQHTIVGQSILKGTPFEPFMVKNGVDATMTEGIVENDYDLPLQMSVHHPQVDVPVLWWRSVGNTHTAFVKETLADEMAAASKQDPVAFRLARLDEKKHARHRAALQLAVDKSGYGKRKLSKGHAWGVAVHESFNTVVAYVVDVSVVKGEPRVHRVTAGVHANRVVNPMSAEAQIQGACIFGLAMTKPGFAIEIENGAVKNSNFPDYPPPRITDAPVVDVFFVPSQENPTGLGEPGVPPIAPAVANALFTLTGKRQRQLPFVMA Sequence number: 277, Sequence length: 154, Organism: Cupriavidus necator H16, PRT MTTLNINVNGKTREVDVDPSTPLLWALRDNLEMTGTKFGCGMAACGACTVHVNGQATRSCVTPVSAAAGARITTIEGIGTDKVGRAVMDAWLKHDVAQCGYCQNGQVMSAIGLLRTKPRPTDADIDQAMAGNLCRCGTYQRIRAAIKDAARALA Sequence number: 278Sequence length: 314, Organism: Cupriavidus necator H16, PRT MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEEELGVEIFERYGKRLTGLTEPGREIVRIVERLLEAENLRQAGDEYAGRHTGRLTVATTHTQARYALPRVVQAFRREYPHVTLALQEASPTHIVELLLTGQADIGIATEALATEAGLTSFEAYSWQHVLVVSPGHPLTRLPEPTLEDVAGFQLITYDAGFTGRRKIDSAFAAEAGLQPEIVLTALDADVIKTYAELDLGVGIIASMAYDERKDSGLVRISADHLFAPNTSVAVRRGAYLRGYAHAFINMFAPHLSPDTVSSALSEQERQALAA Sequence number: 279 Sequence length: 321, Organism: Cupriavidus necator H16, PRT MGIMNDIAEASTSVAHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGERKIELPFPLVHIDTGHNYPEVIAFRDQRVAELGARLVVGHVEDSIKRGTVRLRKETDSRNAAQAVTLLETIGEHQFDALMGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIAREKLALPPIYYSHQREVVRKNGLLVPVTPITPKQDGDVSEVLSVRFRTVGDISTCCPVASVADSPEAIIAETAVTEITERGATRMDDQTSEASMERRKKEGYF Sequence number: 280 Sequence length: 267, Organism: Cupriavidus necator H16, PRTMSTVLSEIAVVDAGAASAMRPPALWVAPEYTGSIAALEQKERELGERLAGIAGRFFRARFASSLAAEDMVLTDAILRGTEAVRAGIRVFTLQTGRLHAETLAVLDKVQAHYGYSIEQFTPDAEAVENYLKKHGLNAFYDSVELRKECCGIRKVEPLNRALSHADAWLTGQRREQAVTRAELPFEEQDEARGIPKFNPLADWSEPEVWAYLKRHNVPVNDLHAKGYPSIGCEPCTRAVRAGEDVRAGRWWWESKDSKECGLHEQNIKH

[0124] Sequence number 281 Sequence length: 434, Organism: Cupriavidus necator H16, PRT MTHQATHQGLLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLTALANAKNKRTAGEQIDFSLLTDGLEAEREQGITIDVAYRYFSTARRKFIIADTPGHEQYTRNMVTGASTADAAIVLVDATRVTVTDGRAELLAQTKRHSAILKLLEIRHVIVAVNKMDLVDYSEQRFNEIRTAYTELAEQLGLKDVRYVPVSALRGDNIVHESDAMAWYQGEPLLPLLEELPVEEAAPEDDAALRFPVQLVIRQDGSQSDDFRGYAGRIEAGTVRVGQKLRVLPANREAVVAEVLTPNGAEASANVGDTVTVRLAEDVDVSRGDMFVAADATTASAKKLQADLCWFDDESLNPARKYVLKHTASVFARVSAVDRVLDVHTLSHETGRHDIRLNDIGSVQISLQKPIVCDAYGDNPATGAFVLIDEATNHTVAAGMIRAFS Sequence number 282 Sequence length: 246, Organism: Cupriavidus necator H16, PRT MPAMRQIVLDTETTGLSAATGDRLIEIGCVELMNRRLTGRLHFYVNPERDIDEGAIAVHGITVEFLADKPRFAEVVNDIREFIQDAELIIHNAPFDLGFLDMEFQLLGLPPFRQHAGNVIDTLREARQMFPGKRNSLDALCDRLGISNAHRTLHGALLDAELLAEVYLAMTRGQDSLVIDMHDSTAGHGGAVAAADLSRLALPVLRATDEEQQAHLALLKGLDKASGGKTVWQEAPAAEPGSLAA Sequence number: 283 Sequence length: 416, Organism: Cupriavidus necator H16, PRTMNLPLNGIKIIDFTHVQAGPACTQLLAWFGADVIKVERPGSGDVTRTQLRDIPDVDALYFTMLNSNKRSLTLDTKKPEGKKILEQLIRESDVLVENFGPGADLRMGFSWERINELNPMIVASVKGFSDGHHYEDLKVYENVAQCAGGASTTGFWDGPPTVSAAALGDSNTGMHLAIGITLALIGRDKTGKGQKVAVSMQDAVLNLCRVKLRDQQRLDRLGYLEEYPQYPHGSFSDVVPRGGNAGGGGQPGWVLKCKGWETDPNAYIYFTIQGHAWEPICKALGKPEWISDPNYATAKARQPHIFDIFNTIEEWLADKTKYEAVDILRKFDIPSPVLSMKEIAADPSLRASGSITEVPHKERGTYLTVGSPIKFSDLKPEITGSPLLGEHSEEVLAGLGYGADDIKRLRESQVI Sequence number 284 Sequence length: 158, Organism: Cupriavidus necator H16, PRT MSTIPITKRGAELLKEELQRLKAVERPAVINAISEARAQGDLSENAEYDAAKEKQAFIEGRILEVEAKLSAAQIIDPTQLDTDGRIVFGATVDLEDLESGSPVSYQIVGDDEADIDVGKISVSSPIARALIGKFEGDVATVLAPGGEREYEVITVRYI Sequence number 285 Sequence length: 291, Organism: Cupriavidus necator H16, PRT MSQYRFIDVQPIAGALGAEISGVDLSQPVPDETFAEIRSALHNHQVIFFRDQQLTPDQHKAFSARFGDLLEVPFVRALEGHAEILPVMKGKAEQTKRNFGGAWHTDMSYAEIPPLGSALYARVIPPYGGDTMWASMYHAYDALSDGLKLVLDKLRAVHSPVRSYGARGAVVNNGDPAHKMDVRTDDRANSEVLHPVVRVHPATGRKALYVNSTYTMRFEGMTEESAPLLQYLYAHAARPEFTCRWFTRGALAVWDNRCTQHLAMNDYDGFDRELHRTTIAGDRPVGVAA Sequence number 286Sequence length: 208, Organism: Cupriavidus necator H16, PRT MKLYYTPGVCSMAVHIALREAGLPVTLAKVDLRRHKLAEPENGTDDYYTINPRGYVPLLQLDDGSRHTEAASLLQYIADLVPERALLPAAGTRERLEATGWITLVSTELHKVFSPWLWHKETAQSTQDECKARLHRRFSELDQHLQTRHYLMGDTFTVADAYCFTIVGWARILKLPLNDYPALQQYLSRVAARPAVQEAMRAEGLVRD Sequence number 287 Sequence length: 242, Organism: Cupriavidus necator H16, PRT MTKQIRLGMLTPSSNTALEPITSAMVAGLPNVSAHFSRFTVTEISLRDQALGQFNLDKILAASLLADARVDVIAWNGTSSGWLGFDKDEALCRQITEATGIPATTSVLALNEILEKTGARNFGLATPYLDDVQQRIIANYERSGFHCVAESHLDRVNYSFAEVEEDTIREMVRGLAQHQPQAITTFCTNLRAAHLAEELEAETGIPLYDTISTVVWKSLRLCGVDTRELRGWGRLFREVE Sequence number 288 Sequence length: 142, Organism: Cupriavidus necator H16, PRT MKITSYYPVIMTGEVAGTAAFYQRHFGFVALFTSDWYVHLQLAGDPSVNLAVLDGTHETIPAPARGQRARGLLLNFEVEDPAVHDRLRAAGLPILLPLRDEAFGQRHFITADPNGVLIDIIKIPPSGEFAAQYEAGALPG Sequence number 289 Sequence length: 134, Organism: Cupriavidus necator H16, PRT MARADVRLCTNSEVFSRNNTMPIYAYRCDACGHGRDVLQKMSDAPLTDCPSCGTAGAFKKQLTAAGFQLKGSGWYVTDFRGGSAGTPTATGAAGGAAAAASTPAAAESPASPASSASATPAAAGGCGSACACH Sequence number 290 Sequence length: 133, Organism: Cupriavidus necator H16, PRTMPAGRDARREAPESVQPGRARRLLPALACAAALLLGAASAMAQPVRAIPDDAQAARLVIATPSAGAPQTATLDGKTVGVAPGLRLFGSDNQLLASAAVAGQKLPVRYKLDLYGQLLTAWVLSEPEQKALKKKS

[0125] Sequence number 291 Sequence length: 401, Organism: Cupriavidus necator H16, PRT MELHLQMGHGMMDHCRVLVGAWRGGTVVLSPRDLDMQQMVRLSEDLARDGGRVLLDPQMFLPQASHPRLVGHAHWAQGGNLQQPATVAHIMERLIDMNAACGAQQIILPGALAAKVDAAWLDAMAQVGECAQRLGVDSARAMQTIALSYDAVRDDKQVEAVVEAMDAWPARTIYLIAEAPSGVYLVNDPTWFANILDIVASARLSGKQVMLGYANQQLLVAACAGANAISSGTWMNVRIFMQNRFLAADEEDRKTRSTWYYAPNLFSEYRIQNLDIAQKLGLLDHLRTPDDFGSGHADVLFSSLQPTLAGFAEPSSFRHFLQCLRTQARAASLGSFESTLSSYRQALDAAQAVLERLDKKSVSSKQRNILASECIDAVRAGLHVLERNHGPRLRRLWGRLS Sequence number 292 Sequence length: 304, Organism: Cupriavidus necator H16, PRT MRFSVYQESRKGGRRVNQDRMGYCFTRDALLMVLADGLGGHAHGEVAAQQALQTLARQFQVQARPTVRNPAEFLQDTIMLAHREIHRYAEANQMADVPRTTVVCCLVQGQQQIYWAHAGDSRYYYLLRKGRLLTRTRDHSKIENNLLQQERVLPMHVANHPERNKLYNCLGSPNLPLIDLGGPVRLEPGDVALLCSDGLWGALEEPALVDKLSRLSVVQAVPALIEQALQNAGEGADNTTGIAMMWELDSNAAGQDSVMTDTLPLNAFTTSILERTGTDSDLLSEEEIERSIAEIRAAIDKTSNLMR Sequence number 293 Sequence length: 348, Organism: Cupriavidus necator H16, PRTMTEPKGTTQPKSAPLPVGTLLSNYRIVKKLASGGFSFVYLATDDTGAPVAIKEYLPASLARRNPGELIPVVPEENAAAFRLGLKYFFEEGRSLARISHPSVVRVVNFFRENSTVYMVMNYELGKTLQEHVLAARQQGGPKVLREHFMRKVFHDLMSGLREVHIHKLLHLDIKPGNIYLREDESPILLDFGAARQTLTMEAARFQPMYTPGFAAPELYGKHSDLGPWTDVYSLGATLYACMAGMPPQEANQREKEDRMGDALARLRSSYTNGLVDLVEWCLRLTPAERPQSVFRLQKELREQTNALGEQALAPQTQAPALAPAERPAATSRFLTLLRRRDEPVPGASVD Accession Number: 294, Sequence Length: 122, Organism: Cupriavidus necator H16, PRT MQIITTRRAPPAVSGAVPASAARAPAASAPQAAAPSARGNAPDLAQAAVAGAAQAAIPSVAASDERELVKRALAEEQRGMNEAIANYARASKAGAGPETLAPLEREVRVRLQKIDMLMRAPH Accession Number: 295, Sequence Length: 219, Organism: Cupriavidus necator H16, PRT MKAKLLHQKHAHWSPESLEQAESGYRQFLKLAAKHPDTPVVPSEQVDAFWHAHILDTRRYASDCERIFGYVLHHDPYVGIDGPEDEARLLQMAAASDALSMREFGSPLTSAAYCARAAADEPAYCARIARTEAPAYCARMAKVEEAAYCARMAKAEEAAYCARIARTDEPAYCARMARAEEPAYCARIARAEEPAYCARIATAGAPAYCALASPQAAAC Accession Number: 296, Sequence Length: 369, Organism: Cupriavidus necator H16, PRTMGVQNEAFLDALFQPIKLGKLELANRMAMAPLTRSRADDNLVPTDMVVEYYSQRASVGLIAEAETQVSTTAQGYTNTPGVYTPEQIAAWKKVTDAVHAKGGRIFLQIWHTGRMSHTHFQPDNQAPVAPSAIAANAKTYINGKGYVECSVPRALETAEIAGIVDDFRTAAANAVQAGFDGVEVHGAHGYLLDAFLRDGTNNRTDAYGGSIENRARFLLEVMAAVVKEIGADRVGVRLAPVSPVNDALESNPQPLFEYVVRELEKLHPVYIHVVEGHTGGPRDNAPFDYEALHRLYTGVWMVNNGYSKEMAEEAIRRGSADMVSFGRKMITNPDLPRRFRENKPLNRPFEDASLYGGNGAHGYVDYPELG Sequence number 297 Sequence length: 403, Organism: Cupriavidus necator H16, PRT MSKRIVVIGAGFAGMWSALGSARLLDEVGRTDVEIVLVAPEPELHVRPRLYEKNPGGMRAPLQEIFQAVGVRFIQGLVERIDVVSQKVELVGLGGDTSRRKLGYDRLVLAAGSRLFRPQIPGLEQHSFNVDQVADAAALDAHLASLARRPESVARNTVVIAGAGFTGIETAAEMPARLREVFGKDAAVNVIMVERNSDVGPDLGPGPRPVITEALTELGVTWKLGSGVAAVDANGVTLENGQRIETDTVIWTAGARASALTSQIPAQRDNFGRLHVDRNLKVKGIDTVYATGDVAYASTDEEGNHAAMSCQHAMKLGRIAGHNVAADLLGATPIPYSQPKYVTCLDLGSWGAVYTEGWDRQVKLVGAEAKALKTQINTEWIYPPSADRAEALAWADPHYVVVA Sequence number 298 Sequence length: 160, Organism: Cupriavidus necator H16, PRTMKRFHVHVHVDDLGKSIAFYSKLFAAEPTRVESDYAKWMLEDPRINFAISTRGQGTGVDHLGFQTDDAAELAELKARAEAADMALLDQGETTCCYARSEKHWVTDPQGIAWEHFHTLGNVPVFGEGKAEAASAEGSACCAPRAPRGKPIGIAVNSNSSCC Accession number: 299, Sequence length: 224, Organism: Cupriavidus necator H16, PRT MRILLAEDNVMLANSLSQALGQAGFTVDCMHDGRSADNLLSTQDYALLILDLGLPGLDGLEVLRRLRQRRNPLPVLILTAHGSVEDRVRGLDLGADDYLAKPFDLSELEARARALIRRAHGHESTQIACGPLHYDSVSRAVLLHGELLPLTGRERAVLEVLLLRDGRAVNKAALSEKIFGIDESVNPDAIEIYVHRLRKKLDGSGVAIVTLRGLGYLLEARPAA Accession number: 300, Sequence length: 571, Organism: Cupriavidus necator H16, PRT MVIDFLASPNLAQFDLASLRYIGGGGAAMPQAVAERLREQFGLNYLEGYGLSETMAPTHSNPSDRPKLQCLGVPTFNTDARVVDPVTLKELPPNEIGEIIVSGPQVFKGYWGKEDATRDAFIEFEGKAFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPDVQEACIIGTRDPYRGETVKAVVVLRAHAKGKTTPEQIIEWAKENMAAYKYPRVVEFVEALPKSGTGKVMWRHLQECENARNTGAEERPAA

[0126] SEQ ID NO: 301 Sequence length: 166, Organism: Cupriavidus necator H16, PRT MSKPAFTLRPATAADSETLFNLILALAEYEKLTHIVEATPQKIQDALFGATPHAEAVLVEVDTDGGRQAVGFALFFHNFSTFLARPGLYLEDLYVDPAWRGHGLGKALLKHLAALAVERGCGRFEWSVLDWNQPSIDFYEAMGADVLPDWRICRVTGEALDKLGAK SEQ ID NO: 302 Sequence length: 345, Organism: Cupriavidus necator H16, PRT MTQATAADAATFAVAVPALGPDVRCHVLQPGRPPLFIEPAGERLLDRAQFRQWSRTVRPALDALILEHGGIVLRGFPTAGTEDFADFIEQFPAFDGGYAGGRAPRETISGRVMEATRLSASVRLAVHSEMAYRRDYPRRIAFFSRKTAEVGGETLITDVRHLAERMDPELAG RLATLGSRTAINFGPRRDADDASYAHMDERGWNQSFHTEDPAEVNRLCAERGLEPVWHEDGSLTVLNALEPFVVHPQTGRKLYRSILHMLPQVENPEQDLERRKRQKYPTGATLGNGERLTDAERAHIDQLCDQTTYSWPWRDGDVMVLDNLQVWHGRNPYQGTRDVQVALLD Sequence number 303 Sequence length: 702, Organism: Cupriavidus necator H16, PRTMASRIVRAACPHDCPDTCALLVTVEDGRAVKVAGDPDHPGTQGVLCTKVSRYTERTYHPDRLLTPMKRIGRKGEKFAPISWDEALDTIATRLQAIARDPQAIVPYSYAGTMGLVQGESMAARFFNKLGASRLDRTICASAGATARALRYTYGASVGMDMEHVVDARLVIIWGGNP IASNLHFWTRAQEAKRRGATLVAIDPYRSLSAEKCHRHIAPMPGTDGALALALIHVLIRDDLLDHDYIERHTVGFEALRERAQAYTPARAAEICGIPAEDIEWLAGLYGQLAVRERQPVAIRLNYGMQVRHGGGQAVRAVACLPSLVGAWRQPAGGLQLSTSGFFPINEAALQRPD LLPGWPQTLPRIVNMSTIGDALLAEPAPGAPRIEAVVVYNSNPVAVAPESGKVAAGFAREDLFTVVLEHFRTDTADYADILLPATTQLEHTDVHKAYGHTYLLANNAAIAPMGEALPNTEIFRRLAQRLGFTEACFADSDEAIAAQAIIPGDARAAGISWESLKEQGWQKLALPAAPFAEGGFPTASGKCQFYSEPMARDGLDPLPDYVPQYEAPLTAPELAARYPLAMISPPARNFLNSSFVNVDSLRATEGEPHLDIHPADAAERGIVHGAMVRVFNDRGSMVARARVTDRARRGLVGLSIWWKKLASDGKNANELTSQRLTDLGRAPVFYDCLVQVEACTEGAVLPA Sequence number 304 Sequence length: 610, Organism: Cupriavidus necator H16, PRTMATLIPALGSVTSRMTSGERRFAERLETKLEDDYLCWYDVAIGEKTRHPDFIVFHPSRGLLVLEVKDWRLDTIREIDQRDVTLLTDRGLVREMNPLEQARQYMFYVTNLLQRDPQLVWPGGSLKGKPFFPYGCGVVLSNITRAQFDKTNLGD VLPPHLVICQDEMYESVDAEHFQQRLWEMFPVKFTFKLSLPQIERIRWHLFPQIRMPEQRALFPEEAAAEPLDMPDILRVMDLQQEQLARSLGEGHRVIHGVAGSGKTMILGYRAEHLAKIVTRPVLVLCYNKTLAAKLAATMIEKGIEEKVHV YNFHAWCSRQLETYNIDRPKGRPSDALFEEMVNRVIQGVDRKAIPAGQYDAVLIDEGHDFRPEWFKLVVQMVNPESNSLLVLYDDAQSIYGGARKRGMTFASVGIQARGRTTILKLNYRNTQEILAVARSFADELLRPTEATEDEAPTVQPVGAGRRGNKPLLIELPNVEAEAERIAQILTDENRAGTPWSEMAVLYRHWDTVGHVTSALTRKGIPFECPQITKRFTPGADSVKVITMHSSKGLEFPLVCIPATGMPHKHDEADEDEARLMYVAMTRATRQLVMTFGDGSRFAGKLSAALGAVRE Sequence number: 305 Sequence length: 413, Organism: Cupriavidus necator H16, PRTMSQRIAIVGAGQSGLQMALGLQANGYRVTLLSNRTPEQIRGGKVMSSQCMFDRALQAERELGLNWWDDACPPVEGIGLAVPHPEQASARAIDWAAPLDRPAQAVDQRLKMPAWMEAFVARGGDLRIADVGPDELEDLTREHDLVLLAAGKGEIVSRFERDASRSPFDKPQRALALTYVTGMQPRTPFSRVCFNLIPGVGEYFVFPALTLSGPCEIMVFEGIPGGPMDRWAEARTPAQHLAESQRILQTYLPWEAERCERVALTDDNGILAGRFAPTVRKPVLTLPSGRLVFGMADAVVVNDPITGQGSNNAAKCCKVYLDAILARGDQPFDQDWMERTFDQYWQYAGDVVAWTNSLLTPPPPHILALLGAAGQAPALAARIANGFDNPPDYFPWWLDPQACHQLINQYVAEAA Sequence number 306, Sequence length: 470, Organism: Cupriavidus necator H16, PRT MRKQQLAVTALLATLLALGGCASGSGSTSMGTSDTAGTNGGTKTTGTDGTGGMGGSGGSTDVAGGGTGGTGGGTGGTGDNNGGNTGGGETPTARTPTAEVINNTGKVLDSTGKTVSDLGDKLGSSNLPLVPTQTQTGLGDAVASAGDAIGALATGLQSGLGSMPNSDNPVGTTVASTGNVVTELGKTLSSTGTAVSGLGTGQLAPLAPLTSPLGTVVNELGKTVSGTGETLTTVLSTGAVEQVTTTTSKLIVPITSQLTGAAQTLGAATGLGAPTTSLLAAVGGALATAGTTLQQTNTPVVSGVGGVVNATGTTVASLGGAVNGPASGGGLGALSPVLAPVTALAGGLTGGAGAGGTGSPLAPVTSLVSSITGGLGSATGGSTGSPLAPVTTAVSGVVNGVVGGVTGAAAGTGTTSPLAPVTNVVAAVTGGATSTAGSTTSKTSTTSATGPIGGLVGGLLGGLTGALGQK Sequence number 307, Sequence length: 619, Organism: Cupriavidus necator H16, PRTMHWFNQLRVTTRLVAGFLVVAVIGAVMGLLGVVNMGRMADWTGKIYNGDLQALKAVQDANINLVYASRAQIGLLSASTMGERAAEKEQIVKSLGAMDERIRQVAGAFEKAEGKALVKQYQELSPFRARMEKYVELISKQPLDTSQFESQVFSE SAELLKDSHALEAVMFQMVKRRDDRARNMEEARSVYDATRLWMLGLVLGGVALSVLLGVLLARALSRQLGGEPGYAAAIAARIAEGDFSAPVATRAGDKGSLVYAMQQMQQQLSRMVRDFKVSAESIGSASREIAAGNNDLSQRTEQQAAALEQ TASSMEELTSTVRQNADNARQASGLAANASETALRGGEVVGRVVQTMDEINDASKKIVDIIGVIEGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRSLAQRSANAAKEIKGLIGDVERVDNGSALVGQAGKTMEEIVQAVKRVTDIMGEI SAASAEQSAGIEQVNQAVAQMDEGTQQNAALVEEAAAAAGALEEQASRLQGAVATFRLAAEDARADSFVAHSLPAATAVPAKVSRAAGAVRAIAAGKRVAKALPASTKAALPAADHAAAAAEAADAVATQPARLVAGRASRPALAAADNGDWSAF Sequence number 308 Sequence length: 320, Organism: Cupriavidus necator H16, PRT MTKVKKIALEEHFMTPGFQAYSKAFTQHIDPAVFAELGRRLADFDEDRLAEMDRAGIDITVLSQTGPGVQGERDAGLAVARARESNDFLAAQIARHPTRFAGFAALPMHDPPEQAARELERAVRELGFKGSLVNGHTHGRYYDDPAYDAFWERMQALDVPMYLHPTDALVQPQVLAGHPELTGAAWGWGVETASHALRLLFGGVFDRFPRLKLILGHMGEGLPYQRWRFDSRFAVYSHGVRLERSPSEYIGTNIVITTSGVCSHGALAGAIAELGAEAVMFSVDYPYESTEVAAKFIEDAPLDDTMRALVCHGNAQRLLKLAccession number 309, Sequence length: 582, Organism: Cupriavidus necator H16, PRT MSSNPQLAGTWPVMSLAEAHARLTAPGAPFETETRVIRGIPTTVWKNAPPTLRELLLNTRAFADRTFVVYEDERVSYDAFRRAAIALAHALVRDGVRKGDRVAVAMRNLPEWPVAFYGALLAGAIVTPLNAWWTGAELEYGLADSGSRVAIVDAERLDRILEHLPGCPALERIYASRAGGTAAEPRVAPLESVIGCPADWAALPDQAPPQVDIDPDDDATIFYTSGTTGKPKGALGTHRNSTNVAVAGSFSPMRNLLRRGEPIPAPDPAAPQKAMLLAVPFFHVTGCMAVLNGAIATGGKIVLMHRWDALRGMELIERERCTAAGGVPTIAWQILEHPERGRFDLSSLENVNYGGAPASPELVRRIGEVFPLAAPGIGWGMTETSSTFTSHSAEEYVMRPDSSGPALPIGEMKVVDGHGRALPPGATGELMARGANVVRGYWNKPEATAQTFADGWLRTGDIARLDEEGYVYIVDRMKDMLIRGGENIYCIEVESTLYEHPAVMDAAVVGLAHKTLGEEPAAVISLKPGMQASEAELQDFVRTRLAAFKVPVRILIYQEMLPRNANGKILKSNLRKLFDAAT Accession number 310, Sequence length: 432, Organism: Cupriavidus necator H16, PRTMTDSTATPAAGLPPPSARTGALSHLRVLDLSRVLAGPWSTQNLADMGADVIKIEKPGEGDDTRHWGPPFLADEAGAPTRQACYFTAANRKRSVTVDMATPEGQELIRELARQSDVVVENFKTGGLKRYGLDYDSLSALNPRLIYCSVTGFGHTGPYAARPGYDLLIQAMSGLMSITGHADGEPGGGPMKVGVAVIDLFTGMYATTAILSALEARHFTGRGQHIDIALLDVAMAVLANQGAGFLNAGDVPRRQGNIHPSVVPYQDFPTADGNMLLAIGNDGQFARFCDAAGVDWARDERYATNTGRVNNRKTLIAMMMELTRTRSTAQWVALLEANSVPCGPINNIAQAHADPHVQHRGLRVEQERYPGAQPPAGEPVNHVVTTASPLRSETPPTLRYAPPALGQHTDEVLRDYLKLSPQQLEALRARRVV

[0127] Sequence number 311 Sequence length: 303, Organism: Cupriavidus necator H16, PRT MQATAATTISPEAYRALDNEAFPFAVRRCTPTIGADVEGIDFREPLDDDTYLSLRRALLKYKVLFFRKQAITPAQHVAVARRFGELEVHPMFTNHPEPELVVFGRDGTTRGRENLYHSDVSWREIPSMGSMLRCVECPEVGGDTMWINMAAAYENLPQDMKARIANLKAVHDAMPTFGSALNEEKYAEMRAKYPPMVHPVVRTHPETGEKILFVNEAFTTHFANFVKEAPYRIGSDYRPAELDLLQYLYRQAAAPEYQVRLRWQPDTIALWDNRSTQHYAVQDYFPAVRHMNRATIIGDRPV Sequence number 312 Sequence length: 262, Organism: Cupriavidus necator H16, PRT MSHWQADAASRPPVLISLTAYGADFALRDGQAALARIAAAGADGVEFRGELQRGHKDEVIQREVARQHGLSVVWSSPEGLWSEAGSLDAAALDRAFTTALALGATRVKMSLGGYREGTALEALLPWLRHDGIELVVENDQTAQAGTVPPLRDFFARITALGENVPMTFDMGNWHWTGEDPLDAAQVFGAGVGYVHCKGVQRTPAKWIAVPLDQSAAPWRSVLRRLPTHVARAIEFPLQGEDLVQVTRHHVELLRSVEVPA Sequence number 313 Sequence length: 494, Organism: Cupriavidus necator H16, PRTMALVADRLGPRRQVLQSHWGGGTPTFLDPGEMRRVMALLHEHFELAAEGEHSIEIDPRRVDHARMALLAELGFNRVSLGVQDFDPEVQQAIHRIQPFEETRAVVDAARTLGFRSVSLDLIYGLPHQTAARFGRTIDQVLALRPDRLSVYSYAHLPHVFKPQRRIDENALPPAGEKLDILVSTIERLSAEGYVYIGMDHFALPDDDLAVAQREGRLQRNFQGYSTHAGYDQVGLGISAIGAIAGRYVQNARTLDEYYGALDHGRLPLARGVAMSADDHLRREIIGALMCNGVLDIPALEARHGIRFGTAFAPELADLAALGADGLVQCAPDRITVTPLGRLLVRRVAMVFDRYLREDAARPASTGAQAVAANDGAQPVRFVPRARYSRVV Sequence number: 314, Sequence length: 161, Organism: Cupriavidus necator H16, PRT MSTESAMFLAPIPTTDAVPPVIERLVEQHQAAWVDQDSIDDWLAGGGDCVLFIAGDPVRFPECVDVAVVLPELQRVFFNGFRIGVAKREREHEDVLANRFGTQRRPSLVFLRDGAYVSVIAGMRDWDEYVREVRRALAMPTSRPPSIGIPVISAAAGGSCH Sequence number: 315, Sequence length: 282, Organism: Cupriavidus necator H16, PRT MNSFPIPVVLLGPGSQAEDETLDYIHMPQGMSVYTPPVLPEPEQIACLTQAQSVLRAILDALDRADPKQPNPRIELWALDEENRQLLKQVLGEGEVSARIEGEYQVYIQEAVFAGVWRVVSTCPDATTDYIEVGAAPEVLRGAARYGANQWSLDVGRVPDGVMNAPSILAEIDDQLLSWHPGKPVHVVNLTLLPMSPQDITFLDETLGAASVTILSRGYGNCRISSTAVPYCWRVVYFNSQDTTILNTVEITDLPEVACAAPEDLRDSHERLTEVLKWVGSI Sequence number: 316, Sequence length: 565, Organism: Cupriavidus necator H16, PRTMTKTTTNPTRFRDTEIRAPRGTTLTARSWLTEAPLRMLMNNLDPEVAENPKELVVYGGIGRAARNWECYDRIVEALTRLEDDQTLLVQSGKPVGVFRTHRDAPRVLIANSNLVPHWANWEHFNELDAKGLAMYGQMTAGSW IYIGSQGIVQGTYETFVEAGRQHFGGNLKGRWVLTAGLGGMGGAQPLAATLAGCSLNIECQQSRIDFRLKTRYVDEQATDLDDALARIDRYTSQGCAISIALCANAAEVLPELVRRGVRPDMVTDQTSAHDPLNGYLPAG WSWDEYRERAQREPAAVVKAAKASMAAHVRAMLAFQQMGVPTFDYGNNIRQMAKEEGVANAFDFPGFVPAYIRPLFCRGIGPFRWAALSGDPQDIYKTDAKVKELIPDDEHLHRWLDMARERISFQGLPARICWVGLGRAKLGLAFNEMVRSGELSAPVVIGRDHLSGSVASPNRETEAMQDGSDWPLLNALLNTASGATWVSLHHGGGVGMGFSQHSGVVIVCDGTDEAAARIARVLHNDPATGVMRHADAGYEIAVDCAREQGLDLPMLNHASRD Sequence number 317 Sequence length: 792, Organism: Cupriavidus necator H16, PRTMARTHILGFPRIGERRELKFAQEAFWRGDRTEAELRDVAAQLRRRHWQLQADRGLDTVATGDFAYYDQMLSLTALLGALPRRFGLDPARLTLTQYFELARGNREQPAMEMTKWFDTNYHYLVPELDAETTFDGGPAWFFEEADEALALGLRARPVLIGPVTYLWLSKSHVAGFDRALLPQLLQGYRRILDQLKARGIEWVQIDEPALCLDLEPAWLDAFTYAALREAGPKLLLATYFDTAADHAQRAAALPVDGFHIDLVRAPQLAAWQAALPAHAVLSLGVIDGRNIWRTDLRRVLDTLRPVQAALGERLWLAPSCSLLHVPVSLAHEVRLDVELKSWLAFATEKLDELSVLGRALNQGDAVVAEALAASDAAQASRRASRRVVNPRVQQR LAAVSAGMADRASPFAERIERQRQALQLPLLPTTIGSFPQTAAIRQTRAAFKRGEIGALEYLERIRAEIAVAVRKQEALGLDVLVHGEAERNDMVEYFGEQLCGYGFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPMKGMLTGPITMLQWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLDWAVTAFRLSASGVQDQTQQIHTHMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVQAMERLLDRACEVVPPQRLWVNPDCGLKTRGWEETEAALANMVSAAARALRARLSARGATTWKRLSKPAAATAAAVPHAGNACTACATHAN Sequence number 318 Sequence length: 450, Organism: Cupriavidus necator H16, PRTMKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEEADVILFNTCSVREKAQEKVFSELGRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRTGQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYRGKMGDTTEIADFALLIEYVAEIPGIERIRYTTSHPKEFTSRLVELYGRCDKLVNHLHLPVQHASDRILMAMKRGYSVLEYKSIIRKLRVLRPDMSMSSDFIVGFPGETDADFEKLMAMIEEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLRRLQRLQATIEENVQRISQNMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQAGRDRLVGQLVDVSITQAFPHSLRGEIVVRQ SEQ ID NO: 319, Sequence Length: 336, Organism: Cupriavidus necator H16, PRT MADFHEFYPLRRHNTFGFDARARFAVHVRSEADLTAALADPRADGLPLLVLGGGSNVVLTGDLDALVLLMEIPGYQPEETGDAWLVTAGAGENWNALVNRTIADGMPGLENLALIPGTAGAAPIQNIGAYGVELRERFEGVRAYDRHTGAFVWLDLRACGFGYRDSLFKHAGAGRYIITAVTLSLPKAWQPVLSYGELARELDGRAAPDAATIRDAVVAIRSRKLPDPAQLGNAGSFFKNPLVSAAQRDALLQANPDLVSYAQPDGSYKLAAGWLIDRCGFKGMTDGPVGVYGKQALVLVHHGGGTGAMLLALANRIADAVQARFGVRIEPEPVVL SEQ ID NO: 320, Sequence Length: 354, Organism: Cupriavidus necator H16, PRTMKRIALTALLAASAGGACAQSAGTIGITLYGRAVAGLDYVNGIARPGGDVDTFRFGSNQYGVSFWGMRGAEDLGGGLRGVFNLEGMITSGTGRSDPLFNRRAQVGLASNDWGTLLLGRAMSLTDDESGAIDPMGLQASSVATLVYGRNWGSRTNAVTYNSPVWGGLSVRAQAGSDGVAGSFNSGKQLSAAVKYAQGNLSLTGIYEELRDTQGRMDNLYVNSRQYTLGGSYALNNLRLFAGYNLTVSSGDTVATPENPYAATRSQMAWVGANYRLLPALTLLSGVYYATLNGGGHGTLVTLGAKYDLSKRTSLYGTVASVFNSRNATFSVEASPDTMPGAGANQQGAYAGIIHLF

[0128] Sequence number 321 Sequence length: 395, Organism: Cupriavidus necator H16, PRT MLSWDDDVQATPQAAPQPALQPAAVAATADQQGVLPPAATQPGILGDNPNAAAAQSNRRVNAADKRVINGSTDVNQLVPFKYKWAWEKYLAGCANHWMPQEINMSRDIALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYLLRQAFEEAIHTHAYQYIVESLGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIMEGLFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFKAEITELFKKAVDLEYRYAEDTMPRGVLGLNAPMFKSYLRFICNRRCQQIGLDQLFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD Sequence number 322 Sequence length: 332, Organism: Cupriavidus necator H16, PRT MSTNTIAVDVGYGNTKFAFPLGADVAASMFPSLAPTRSASSLASHGGGYFQARDVVHVTVDGAEYEVGPDVSITSAYGNNGRTLSEDFVTTPEYAALLFGALHYSQARDVGQLILGLPVHTLQKYAGALQERFTGAHDFGAGDVTIKRVVALPQPLGSLVTFMRQSGKELDPDDNCLIVDVGYFTTDWVVARGYMMDDTRSGGVPGGSSRIYKQVATLLSADEGGEPTGDIERIDKALRQGKLMRYYEKMVDLRPYFEVAKAQCQMAVKEMQTRVGRTEDIAAIVLTGGSALYSGAIRAAFPRSHIVAMDSPCFANVRGFFDIGSARQARG Sequence number 323 Sequence length: 257, Organism: Cupriavidus necator H16, PRTMKAVILAGGLGTRIAEESDTKPKPMVEVGGRPLLWHIMKIYAHHGIKDFIICLGYKGYVIKEFFFNYYRHMADMQIDLKTGDHQILNSQSEDWRITLVDTGSETMTGGRLKRVASHLGNDTFCLTYGDGLSNIDITAELQFHRRHGKLATVAAVQPPGRFGVLNLDANQNVSSFEEKPRDEIGWINGGFFVLEPSVVRYIEDDATSWEAAPLMNLAKDGQLAAFQHTGFWQPCDTLRDKRELESLWNKRKAPWRIWE SEQ ID NO: 324, Sequence length: 646, Organism: Cupriavidus necator H16, PRT MHLFDHSDMTQHDIRAEQAIASATDASITSDAAQSPLDKLDALLNPSPAVEEAPAVESGFATLGLDAAILRALSELNYNTPTPVQAQAIPAFLAGRDLLVSSQTGSGKTAAFMLPAIQRISEMPAPQRATEPAKRMKGKRPRPSPAQPALLVLTPTRELALQVTEAAAKYGRNLRRIVCASILGGMPYPKQLAALAKMPDILVATPGRLLDHIDAGRIDLSALQMLVFDEADRMLDMGFADDIDAIVNATPATRQTLMFSATLDARIAQLASRQLRDPQRIEIAAARADQSHIEQRLHFTDDMSHKERLLDHLLRDATLKQAIVFTATKRDADSLAERLSDTGFSAGALHGDMTQGARNRTLTALRRGNLRVLVATDVAARGIDVPDITHVVNFDLPKQAEDYVHRIGRTGRAGRSGTAINLVNHNDMFQWKRIERFTNQRIDASVIEGLEPRRSPKPRSNFGGKPGGGRGDFRGNGGGYRGGERSFGDRKFGGGESRTGFGERKFSGESRGFGNRPEGARPFGDDNRGGFGNREGGYRGQGQGGQGAGYRGANDGARGFGNREGGRSFGDDNRGGFGNRDGAAPRSFGDNNRGGFGDRTGGGYRGNTGGNGDGRSFGNRDGNRDGNRSFGGNGSGNRNSRSRYER SEQ ID NO: 325, Sequence length: 384, Organism: CupriavidusNecator H16, PRT MNALVNLLDLDADALTAYCGELGEKPFRARQLQRWIHHYGASRFDAMSDLAKSLREKLATRAEIRAPAAITDHLSADGTRKWLLDVGQGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIIGQLWMAEFAMREQLGRGPKDDRVISNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLNKKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNDQIRRFAQVLMDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLVERGKFGKITPLVPVAASGQPREVRPA Sequence number 326, Sequence length: 238, Organism: Cupriavidus necator H16, PRT MARRNAASPQMGLDLAPAAAAVQRLCGVDEAGRGPLAGPVYAAAVILDPKRPIRGLADSKILTAAKREQLYEKICERALGWHIAFATVEEIDTLNILHASMLAMQRAVQGLAASGIVPDLVQVDGNRCPRVPFPVEAIVQGDALVKAISAASILAKVARDRELRVLHERYPQYGFDSHVGYGTPQHLAALAEFGATPHHRRSFAPVREALARLPMFTAMPATAEVIEPVATVSVTAAQ Sequence number 327, Sequence length: 656, Organism: Cupriavidus necator H16, PRTMTGSVQRTAAKTRETGAIKDPGQTSAGSTAQPDPSTEAAARSQQLRALIQLGRQRGYLTHADISDHLPENFTDTAAMESIVSTFAEMGVKIYEQTPDAETLLLSDGPVASDDQADEEAEVALATVDSEFGRTTPVRMYRMEMSSATLLTRKQEVEIAKRIEE GLNNMVHAISACPFTIAAILELSGKVASNEISDDLVDGLSDESIAEAAVAAAADETDDSVDSADEADDDSEDSDDEGSTQQSNEKALAQLREECLKRFARVTAQFELMCQESAANGAGSAAFLAARDAVREELRTIRFTAKTIERLCANVQAMVDEVRTVERQ VVQLLVERCGMEREEVIARFPGNETNLAWGQELVAQSRPYSAAVARALPDLEAHQQKLIDIQARAALSLPDLKGVNRKMLAAERQMRQAKHEMTQANLRVISIAKKYTNRGMLFLDLIQEGNIGLMCAVDKFEYRRGWKFSTYATWWVRQAVTRAIADQARTIRVPVHMIEQINKLNRLSREIMQQTGKEPDPAVLAERLDMTEDKVRSIMKIAKEPVSMETPVGEDGDTSLGDMIADSDTATPADAALQAGLRAVVREMLDELTPREAKVLRMRFGIDMSTDYTLEEVGKQFDVTRERIRQIESKAMKKLRHPSRADQLITYLRDA Sequence number 328 Sequence length: 70, Organism: Cupriavidus necator H16, PRT MTTIRLKENEPFEVAMRRFKRTIEKNGLLPELRAREFYEKPTAARKRKAAAEKRHYKRIRSQMLPKKLY Sequence number 329 Sequence length: 784, Organism: Cupriavidus necator H16, PRTMSNLPASVSQKIVSLIAAELSVQPRQVAAAVALLDEGATVPFIARYRKEVTGNLDDTQLRNLEERLLYLRDMEDRRAAILASIQGKLTPELQAAIEAAETKQALEDLYLPYKPKRRTRAQIARECGLEPLALALLADPTLDPQAEAARYVNGNPTADGGVPVKAALDGARDILSEQFGETAELLGKLREHLWT NGVVSSTVMEGKETAEEEKFRDYYAYSETIRTVPSHRALALFRGRNAGVLMVKLGLGEEQDAMVPPHPCEGMIARHVGIQQLGRPADKWLGDVCRWCWRVKVQPHLETELLTQLRETAENEAIKVFGRNLHELLLAAPAGPKSVMGVDPGIRTGCKIAVVDSTGKLLDTATIYPHEPRRDWNGSLATLARQHNV ALVSIGNGTASRETDKLVQDLMKQMPELKLTKIVVSEAGASVYSASELAAKEFPDLDVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDVNQRELARALDAVVEDCVNAVGVDVNTASAPLLARVSGLNTVLARNIVEFRDANGAFPNRNALKQVPRLGDKTFEQAAGFLRINDGDNPLDRSSVHPEAYPVVQRILEHVKKGLRDVMGNRDALRGLSPTQFTDDSFGLPTVRDILSELEKPGRDPRPEFKTATFQEGVEDVKDLQPGMVLEGVVTNVAAFGAFVDIGVHQDGLVHISALSSKFVKDAHEVVKAGQIVKVKVMEVDVKRNRIGLSMRLDDEPGQAAARAGGDRGDRGGQRNGGKGFNGGRREAEPASAMAAAFAKLKR Sequence number: 330 Sequence length: 626, Organism: Cupriavidus necator H16, PRTMARTAPAASFESLESDLDQKFAYPASSKTYLTGSRPDIRVPLRTILQTSTRTDKGEMQNPPIPVYDTSGPYSDPDVHIDLKAGLPAVRAKWIEERGDTEVLPGLSSEYGRDRANDPATAHLRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRESLNLQALYDKPDYKALLRQHPGNALGAGLPLRPEDITPEFVRQEIASGRAIIPANINHTELEPMAIGRNFRVKINGNLGNSAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGIAEDLTWEMF RDTLIEQAEQGVDYFTIHAGVLLRYVPLTADRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADSNDDAQFGELRTLGELTAKAWKHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAP GYDHITSGIGAANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPERARSYHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLPKEAQQGMEEKSIEFLKKGSKIYS

[0129] Sequence number 331 Sequence length: 282, Organism: Cupriavidus necator H16, PRT MQIVNISAYKFVSLDDIETLRPAMRERCEAAGLKGTILLAPEGINMFLAGPRAAIDGFMDWLHADARFADIAPKESLSENQPFKRMLVRAKKEIITMKMPLIRPEEGRAPSVRPAELKRWLDQGHDDEGRPVVMLDTRNDFEVAVGTFEDAVEYDIAKFSEFPEAVAAHKAELEGKTVVSFCTGGIRCEKAAIHMQEVGVERVYQLEGGILKYFEEVGGSHYRGDCFVFDYRTALNPNLEPAGPKQCFACRAVVTPQEQQSPHYVVGKSCPHCIGSKDQAAA Sequence number 332 Sequence length: 314, Organism: Cupriavidus necator H16, PRT MSHSNNFYRLETRLQSQTGKAIGDFGMIEDGDTLVCMSGGKDSYTMLSVLMALQKRAPIKFCLIAMNLDQKQPGFPEHILPEYLKSVGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVQTFFLNMFFG GKMKAMPPKLSTDDGQHIVIRPLAYCSEKDIASYARAMEFPIIPCNLCGSQENLQRKKVSEMLQDWERQNPGRIDNIFSALRNVVPSHLADTDLFPFTGLATGLAKVDEASLFGETTFQQQPLMFAGNVEENRMEFVRFERPPAAAAAPAAEQAGTQ

[0130]

[0131]

[0132] According to the method for producing isopropanol of the present invention, which uses a recombinant microorganism derived from a hydrogen-oxidizing bacterium belonging to the genus Capriavidus, isopropanol can be efficiently produced from carbon dioxide as a carbon source.

Claims

1. A method for producing isopropanol from a carbon source using a recombinant microorganism derived from a bacterium belonging to the genus Capriavidus, the method comprising: (1) a first step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus until a change in the expression level of an indicator biomolecule occurs; and (2) a second step of culturing the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus cultured in the first step while maintaining the change in the expression level of the indicator biomolecule to produce isopropanol, wherein the recombinant microorganism derived from a bacterium belonging to the genus Capriavidus is a recombinant microorganism into which a gene for acetoacetate decarboxylase and a gene for alcohol dehydrogenase have been introduced.

2. The production method according to claim 1, wherein the bacterium of the genus Cupriavidus is selected from the group consisting of Cupriavidus necator, Cupriavidus pinatubonensis, Cupriavidus laharis, and Cupriavidus metallidurans.

3. The production method according to claim 1, wherein the bacterium of the genus Cupriavidus is Cupriavidus necator.

4. The method of claim 1, wherein the acetoacetate decarboxylase gene is a or b, and the alcohol dehydrogenase gene is c or d. a: A gene encoding the amino acid sequence shown in SEQ ID NO: 1 b: A gene encoding an amino acid sequence having 90% or more identity with SEQ ID NO: 1 c: A gene encoding the amino acid sequence shown in SEQ ID NO: 2 d: A gene encoding an amino acid sequence having 90% or more identity with SEQ ID NO: 2 5. The method of any one of claims 1 to 4, wherein the indicator biomolecule is a protein or a compound.

6. The manufacturing method according to any one of claims 1 to 5, wherein the fluctuation is an increase.

7. The manufacturing method according to any one of claims 1 to 5, wherein the fluctuation is a decrease.

8. The method of any one of claims 1 to 7, wherein the indicator biomolecule is one or more proteins or compounds selected from Group A. Group A: Proteins and compounds comprising: one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 79 and 249 to 265; a protein comprising an amino acid sequence having 90% or more identity to one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 79 and 249 to 265; and one or more compounds selected from glutamic acid, tyrosine, serine, leucine, and isoleucine.

9. The production method according to claim 8, wherein the one or more proteins or compounds selected from Group A are: a protein shown in SEQ ID NO: 3 to 26, 249 or 250; a protein comprising an amino acid sequence having 90% or more identity to one or more proteins selected from the proteins shown in SEQ ID NO: 3 to 26, 249 or 250; and glutamic acid.

10. The method according to claim 8 or 9, wherein the carbon source is one or more selected from the group consisting of sugars, carbon dioxide, glycerol, acetic acid and formic acid.

11. The method of claim 10, wherein the carbon source is carbon dioxide.

12. The process according to claim 11, which is carried out under hydrogen / carbon dioxide / oxygen conditions.

13. The method according to claim 12, wherein the concentration of hydrogen is 1 to 8% of the total gas in the reaction system.

14. The method of claim 12 or 13, wherein hydrogen is supplied by a proton exchange membrane (PEM) water electrolyzer using a manganese-iridium oxide composite (Mn-IrOx) anode.

15. The method for producing isopropanol according to any one of claims 1 to 14, wherein the yield of isopropanol per unit of electricity is 45% or more.

16. The method of claim 8 or 9, wherein the carbon source is glucose.

17. An isopropanol production apparatus for use in the production method according to any one of claims 11 to 14, characterized in that it comprises a PEM-type water electrolysis apparatus and a closed circulation type culture apparatus.

18. A method for using one or more proteins or compounds selected from the following Group A as biomarkers in a method for producing isopropanol using a microorganism: Group A: proteins and compounds including: one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 79 and 249 to 265; proteins comprising an amino acid sequence having 90% or more identity to one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 79 and 249 to 265; and one or more compounds selected from glutamic acid, tyrosine, serine, leucine, and isoleucine.

19. One or more proteins or compounds selected from Group A below, which are used as biomarkers in a method for producing isopropanol using a microorganism: Group A: one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 26, 249, or 250; a protein comprising an amino acid sequence having 90% or more identity to one or more proteins selected from the proteins set forth in SEQ ID NOs: 3 to 26, 249, or 250; and glutamic acid.

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