Device for discharging bubbles from bagged stem cell formulation and method for calculating stress of bagged stem cell formulation

Abstract

A device for discharging bubbles from a bagged stem cell formulation and a method for calculating the stress of a bagged stem cell formulation. The device comprises a bottom plate (1) and a vibration motor (2); the vibration motor is fixedly connected to the bottom plate; a formulation container (3) filled with a liquid and a plurality of connecting blocks (4) are arranged above the bottom plate; the connecting blocks are arranged outside the formulation container; and a buffer spring (5) is arranged to be fixedly connected to the formulation container. In the device, a buffer spring and a formulation container filled with a buffer liquid are mounted on the upper side of a support plate, so that stem cells in a bagged formulation can be sufficiently buffered and protected during transportation, storage or treatment, thereby avoiding damages to the stem cells due to external impact or vibration. A vibration motor drives the support plate to vibrate, and springs having different heights are provided on the left and right sides, so that the bottom plate has a certain inclination angle, thereby increasing the vibration amplitude, and thus effectively improving the discharging efficiency and ensuring that the gas in the bagged formulation can be quickly and effectively discharged.

Description

A stem cell bag preparation bubble exhaust device and a stem cell bag preparation stress calculation method TECHNICAL FIELD

[0001] The present application relates to the technical field of stem cell preparation, in particular to a stem cell bag preparation bubble exhaust device and a stem cell bag preparation stress calculation method. BACKGROUND

[0002] Stem cell bag preparation refers to a preparation that encapsulates stem cells in a sterile and sealed bag after a specific preparation and preservation process. This preparation is commonly used for medical purposes, such as treating various diseases, conducting scientific research or drug development, etc. Stem cell bag preparation has the advantages of easy storage and transportation, and can maintain the activity and stability of cells for a long time. In addition, bag preparation can reduce the risk of cell contamination during transportation and storage, and improve the safety of treatment.

[0003] However, air bubbles are easily produced during the production of stem cell bag preparation. The presence of air bubbles in stem cell bag preparation occupies space in the bag preparation, resulting in a decrease in the effective volume of stem cell preparation. It also affects the concentration and uniformity of stem cell preparation, thereby affecting its therapeutic effect. In addition, it can become a hiding place for bacteria, viruses and other microorganisms, increasing the risk of contamination of stem cell preparation. Therefore, how to effectively remove air bubbles in stem cell bag preparation is a key technical problem.

[0004] Existing air bubble removal techniques usually use a combination of DC motors and vibration motors to perform multi-dimensional shaking on cell preparation bags to improve air bubble removal efficiency. Although this method is effective, it can cause damage to stem cells when they are subjected to excessive stress, thereby affecting the effectiveness of subsequent stem cell experiments. Therefore, there is an urgent need for a device that can effectively remove air bubbles while buffering stem cells to avoid damage.

[0005] The invention patent CN202222012151.1 solves the problem of the existing technology that the cell preparation is usually shaken and exhausted by manual operation, which is time-consuming and labor-intensive, affecting the preparation efficiency of cell preparation. It fixes the cell preparation bag to the fixed plate, then drives the driving rod and the knocking rod to rotate through the electric drive, thereby knocking the fixed plate, making the fixed plate shake, and making the cell preparation bag shake, so that the air bubbles in the cell preparation shake out. Although this method has a simple structure, it is easy to damage stem cells due to poor control of the knocking rod driven by manual and electric operation. SUMMARY

[0006] To overcome the shortcomings of the prior art, the present application solves the problem that the existing stem cell bag preparation bubble exhaust device easily damages stem cells.

[0007] This invention provides a bubble-removing device for stem cell bagged preparations, including a base plate and a vibration motor. The vibration motor is fixedly connected to the base plate. A preparation container containing liquid and multiple connecting blocks are arranged above the base plate. The connecting blocks are arranged outside the preparation container and are fixedly connected to the preparation container with buffer springs.

[0008] Furthermore, the base plate is inclined, with the angle between the base plate and the ground being 15 to 45 degrees.

[0009] Furthermore, multiple shock-absorbing springs are fixedly installed below the base plate, and the lower ends of the shock-absorbing springs are fixedly connected to a fixing plate.

[0010] As a preferred embodiment, the buffer spring is arranged in the same direction as the vibration motor.

[0011] As a preferred embodiment, the damping spring on one side of the base plate is higher than the damping spring on the opposite side.

[0012] As a preferred embodiment, the height ratio of the damping springs on opposite sides of the base plate is 1.2 to 2.

[0013] This invention also provides a method for calculating the force on the stem cell bagged preparation in the aforementioned bubble-removing device, and obtaining the excitation force of the vibration motor. and the radius of the formulation container The stress on the bagged stem cell formulation Through formula Perform the calculation.

[0014] As a preferred option, the excitation force of the vibratory motor during vibration... The increase is carried out in three stages, with the first stage being the excitation force. The maximum external force that the stem cell bagged formulation can withstand is 0.4 times that of the second stage, which is the excitation force. The maximum external force that the stem cell bagged formulation can withstand is 0.6 times that of the third stage, which is the excitation force. The maximum external force that a 0.75 times larger bagged stem cell formulation can withstand.

[0015] As a preferred approach, the first phase lasts 8 to 12 minutes, the second phase lasts 13 to 17 minutes, and the third phase lasts 4 to 6 minutes.

[0016] Furthermore, the second and third stages are carried out alternately until the designed value of bubble content in the stem cell bag formulation is reached.

[0017] The beneficial effects of this invention are as follows:

[0018] 1、The present application ensures that the stem cells in the bagged preparation can be fully buffered and protected during transportation, storage or processing, thereby avoiding damage to the stem cells due to external impact or vibration, by installing a buffering spring on the upper side of the support plate and a preparation container containing a buffering liquid.

[0019] 2、The present application can increase the vibration amplitude and effectively improve the exhaust efficiency by vibrating the support plate through the vibration motor and setting the springs on the left and right sides with different heights, so that the bottom plate has a certain angle of inclination, thereby ensuring that the gas in the bagged preparation can be quickly and effectively exhausted. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to make the content of the present application more easily and clearly understood, the present application will be further described in detail below according to specific embodiments and in conjunction with the accompanying drawings, in which

[0021] Fig. 1 is a structural schematic view of the present application.

[0022] Fig. 2 is a relationship curve between the stress of the stem cell bagged preparation and the excitation force in the embodiment.

[0023] The reference numerals in the drawings are as follows:

[0024] 1, bottom plate; 2, vibration motor; 3, preparation container; 4, connecting block; 5, buffering spring; 6, shock absorbing spring; 7, fixed plate. DETAILED DESCRIPTION

[0025] In order to make the content of the present application more easily and clearly understood, the present application will be further described in detail below according to specific embodiments and in conjunction with the accompanying drawings, in which

[0026] Embodiment:

[0027] Referring to Figs. 1-2, the present embodiment provides a high-efficiency stem cell bagged preparation bubble exhaust device, which solves the problem that the existing stem cell bagged preparation bubble exhaust device is easy to damage the stem cells; a stem cell bagged preparation bubble exhaust device, comprising a bottom plate 1 and a vibration motor 2, the vibration motor 2 is fixedly connected to the bottom plate 1, a preparation container 3 containing liquid and four connecting blocks 4 are arranged above the bottom plate 1, the connecting blocks 4 are arranged outside the preparation container 3 and are provided with buffering springs 5 fixedly connected to the preparation container 3.

[0028] Four shock absorbing springs 6 are fixedly arranged below the bottom plate 1, the lower end of the shock absorbing spring 6 is fixedly connected to a fixed plate 7, one side of the shock absorbing spring 6 is higher than the shock absorbing spring 6 on the opposite side, specifically, the height ratio of the shock absorbing springs 6 on the two opposite sides is 2, by the height difference, the bottom plate 1 is arranged at an angle, and the angle between the bottom plate 1 and the ground is 45 degrees.

[0029] The present embodiment also improves the applicability of the device, and the buffering spring 5 is arranged in the same direction as the vibration motor 2.

[0030] Furthermore, this embodiment also provides a method for calculating the force on the stem cell bagged preparation when using the above-mentioned degassing device, specifically including the following steps: first, obtaining the excitation force of the vibration motor 2. In this embodiment, a VBBX-25186-B model vibration motor and a radius of the preparation container 3 are selected. When the formulation container 3 is selected as 50mm, the force on the stem cell bag formulation is... Through formula Calculations were performed to obtain a stress and excitation force relationship for the stem cell bagged formulation. The relationship curve between them is shown in Figure 2 in this embodiment.

[0031] The excitation force of the vibration motor 2 during the specific vibration process The increase is carried out in three stages, with the first stage being the excitation force. The stem cell bagged formulation, at 0.4 times the strength, can withstand a maximum external force for 10 minutes; the second stage involves an excitation force of [value missing]. The stem cell bagged formulation, at 0.6 times the strength, can withstand a maximum external force for 15 minutes; the third stage involves an excitation force of [value missing]. The maximum external force that the 0.75 times stem cell bagged formulation can withstand lasts for 5 minutes, and the second and third stages alternate until the designed value of bubble content in the stem cell bagged formulation is reached.

[0032] The above embodiments and accompanying drawings are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. The present invention has been described in detail with reference to preferred embodiments. Those skilled in the art should understand that any changes, modifications, additions, or substitutions made by those skilled in the art within the scope of the present invention do not depart from the spirit of the present invention and should also fall within the protection scope of the claims of the present invention. Other related technical structures not disclosed in detail in the present invention are existing technologies in the art.

Claims

1. A stem cell bag preparation exhaust bubble device, comprising a bottom plate (1) and a vibration motor (2), the vibration motor (2) is fixedly connected to the bottom plate (1), characterized in that, a preparation container (3) containing liquid is arranged above the bottom plate (1), and a plurality of connecting blocks (4) are arranged outside the preparation container (3) and fixedly connected to the preparation container (3) through buffer springs (5).

2. The stem cell pocket formulation deaeration bubble device of claim 1, wherein, The bottom plate (1) is arranged obliquely, and the included angle between the bottom plate (1) and the ground is 15-45 degrees.

3. The stem cell pocket formulation de-aeration bubble device of claim 1, wherein, A plurality of shock-absorbing springs (6) are fixedly arranged below the bottom plate (1), and the lower ends of the shock-absorbing springs (6) are fixedly connected to a fixed plate (7).

4. The stem cell pocket formulation de-aeration bubble device of claim 1, wherein, The arrangement direction of the buffer spring (5) is consistent with the direction of the vibration motor (2).

5. The stem cell pocket formulation de-aeration bubble device of claim 1, wherein, The shock-absorbing springs (6) arranged on one side of the bottom plate (1) are higher than those arranged on the opposite side.

6. The stem cell pocket formulation venting bubble device of claim 1, wherein, The height ratio of the shock-absorbing springs (6) on the opposite sides of the bottom plate (1) is 1.2-2.

7. A method for calculating the force on a stem cell pocket preparation in a bubble trap according to any one of claims 1 to 6, characterized in that, Obtaining the exciting force of the vibration motor (2) and the radius of the preparation container (3) , the stress of the stem cell bag preparation is calculated by the formula ​ 8. The method of claim 7, wherein the method is for calculating the force of a stem cell pocket preparation, and wherein the method further comprises: determining a force of the stem cell pocket preparation based on the determined volume of the stem cell pocket preparation and the determined volume of the stem cell pocket preparation. The exciting force of the vibration motor (2) during the vibration process The exciting force is increased in three stages, the first stage being 0.1 times the maximum external force that the stem cell bag preparation can withstand The second stage is 0.4 times the maximum external force that the stem cell bag preparation can withstand The third stage is 0.6 times the maximum external force that the stem cell bag preparation can withstand The third stage is 0.75 times the maximum external force that the stem cell bag preparation can withstand 9. The method of claim 8, wherein the method is for calculating the force of a stem cell pocket preparation, and wherein the method further comprises: determining a force of the stem cell pocket preparation based on the determined volume of the stem cell pocket preparation and the determined volume of the stem cell pocket preparation. The first stage lasts for 8-12 minutes, the second stage lasts for 13-17 minutes, and the third stage lasts for 4-6 minutes.

10. The method of claim 8, wherein the method is for calculating the force of a stem cell pocket preparation, and wherein the method further comprises: determining a force of the stem cell pocket preparation based on the determined volume of the stem cell pocket preparation and the determined volume of the stem cell pocket preparation. The second stage and the third stage are alternately performed until the designed value of the bubble content in the stem cell bag preparation is reached.

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