Topical composition containing yeast extract

Abstract

[Problem] Yeast extract is a natural product, and therefore often has had a problem in stability. Under these circumstances, the present invention addresses the problem of providing a composition in which the stability and clarity of yeast extract are secured. [Solution] The present inventors have made extensive studies. As a result, it has been found that the problem can be solved by dissolving yeast extract, the yeast extract being extracted from yeast cells with an organic solvent such as ethanol and containing a yeast-derived phospholipid or the like, in a specific solvent. This finding has led to the accomplishment of the present invention.

Description

External composition containing yeast extract

[0001] The present invention relates to the use of yeast extract in cosmetics.

[0002] Yeast extracts are used in a variety of applications, including as foods and seasonings. They are also known to be useful as oral preparations for moisturizing the skin (Patent Document 1). Furthermore, because yeast extracts contain ceramides, phospholipids, and the like (Patent Documents 2 and 3), they have skin moisturizing properties and can therefore be used in cosmetics.

[0003] JP 2019-216617 A JP 2012-246287 A WO2015 / 141507

[0004] Since yeast extract is a natural product, stability issues often arise. Therefore, an object of the present invention is to provide a composition that has the stability and clarity of yeast extract.

[0005] As a result of extensive research, the present inventors discovered that the above-mentioned problems can be solved by dissolving a yeast extract containing yeast-derived phospholipids and the like in a specific solvent, thereby completing the present invention. (1) A topical composition containing a yeast extract and one or more solvents selected from a dihydric alcohol, a polyhydric alcohol, and a polyol. (2) The topical composition of claim 1, wherein the yeast extract of (1) is an organic solvent extract. (3) The topical composition of claim 1, wherein the dihydric alcohol, polyhydric alcohol, or polyol of (1) is pentylene glycol, glycerin, propylene glycol, 1,3-butylene glycol, or dipropylene glycol. (4) A cosmetic comprising the topical compositions of (1) to (3). (5) A topical skin preparation comprising the topical compositions of (1) to (3).

[0006] The topical composition containing the yeast extract of the present invention can be extracted from yeast, which is known as a food product, and therefore can be produced relatively easily and inexpensively and can be used safely. Furthermore, the present invention provides a material that has improved storage stability and clarity and is suitable for use as a raw material for topical preparations such as cosmetics.

[0007] The yeast used in the production of the yeast extract of the present invention is not particularly limited as long as it contains a yeast cell wall. Any yeast commonly used in the production of foods and cosmetics can be used. Specific examples include baker's yeast, brewer's yeast (Saccharomyces cerevisiae), and Torula yeast (Cybalindonella jadini; also known as Candida utilis). Among these, Torula yeast is particularly desirable. Yeast cells obtained by culturing yeast may be used as is, or cells rich in cell wall fractions produced during typical yeast extract production processes may be used. Cell wall fraction-rich cells produced when Torula yeast cells are extracted with an aqueous solvent are particularly desirable. Dried, undried, or slurried yeast can be used, with no particular restrictions on the water content. For the subsequent extraction step, it is preferable to use dried yeast or yeast that has been separated into solids by centrifugation or the like. Commercially available yeast cells may also be used.

[0008] An extract is obtained from the yeast cells. The extraction process can be performed using methods such as organic solvent extraction, enzymatic treatment followed by organic solvent extraction, or supercritical fluid extraction. For example, in the case of organic solvent extraction, solvents such as methanol, ethanol, n-propanol, hexane, acetone, and chlorinated solvents are used. Lower alcohols with 1 to 3 carbon atoms are preferred, and among these, solvents suitable for food and cosmetic applications are preferred. Ethanol is a particularly preferred solvent. Also included in the present invention is hydrous ethanol, which is a mixture of industrially used water such as distilled water. The phospholipid content of the resulting phospholipid composition can be adjusted by varying the water content. Therefore, hydrous ethanol is used according to the intended purpose. The water / ethanol volume ratio is typically 0.01 / 99.99 to 30 / 70, preferably 0.5 / 99.5 to 30 / 70. The water content can be determined by taking into account the water content of the raw material. The amount of ethanol used is not particularly limited. It is usually approximately 1 to 30 times, and more preferably 1 to 10 times, the weight of the yeast cells used as the raw material. The extraction method involves adding ethanol, thoroughly stirring, and then carrying out the reaction at an appropriate temperature for an appropriate time. The temperature is not particularly limited, but is usually between 20°C and less than 80°C, preferably between 50°C and 70°C. The extraction time is not particularly limited, but is usually at least one hour, preferably 3 to 9 hours. The extraction method is also not particularly limited, but is usually selected from stirring, reflux, immersion, shaking, ultrasonic extraction, etc.

[0009] When enzyme treatment is performed before extraction, the extraction time can be shortened. Examples of enzymes include "Denazyme GEL" (manufactured by Nagase ChemteX Corporation), a cell wall-digesting enzyme derived from the genus Streptomyces, "Filtrase BRX" (manufactured by DSM Japan), a β-glucanase derived from the genus Taloromyces, and "Tunicase FN" (manufactured by Amano Enzyme). Tunicase FN is preferred. There are no particular limitations on the enzymatic reaction conditions, and they can be selected as appropriate by a person skilled in the art depending on the enzyme used.

[0010] In the case of supercritical extraction, the yeast suspension is brought into contact with carbon dioxide in a supercritical state, and then the pressure is reduced to atmospheric pressure, thereby separating the carbon dioxide from the yeast suspension.

[0011] The extraction step may be carried out not only once but also multiple times, or the methods up to the previous steps may be combined. Fresh solvent may be added to the yeast cells after extraction, and the extraction step may be carried out again, or the solvent may be brought into contact with the yeast cells multiple times.

[0012] After the extraction step, the cell residue is separated and removed to obtain a yeast extract. The separation method is not particularly limited, and any known method can be used. For example, known methods such as centrifugation, filtration centrifugation, suction filtration, filter press, cylinder press, decanter, etc. can be used.

[0013] The obtained yeast extract may be subjected to a drying and concentration process, if necessary. The drying process may be a method employed in food production and cosmetic ingredients, such as a general drying process such as spray drying. The concentration process may also be performed by any method, and may be a method that can be employed by those skilled in the art in food production and cosmetic ingredients.

[0014] The yeast extract of the present invention is a composition containing phosphatidylcholine, phosphatidylethanolamine, lysophosphatidylcholine, N-acylphosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, α-linolenic acid, linoleic acid, oleic acid, palmitoleic acid, palmitic acid, heptadecenoic acid, etc. Because it contains these components, cosmetics containing the yeast extract of the present invention can exhibit skin-improving functions such as moisturizing and wrinkle reduction.

[0015] The yeast extract of the present invention contains the components described above. More specifically, the phospholipids in the yeast extract contain 20% by mass or more of the compound of general formula 1, and further, among the compounds of general formula 1, phosphatidylcholine (PC) having a choline residue accounts for 40% by mass or more of the phospholipids (PC / total phospholipids), and α-linolenic acid accounts for 20% by mass or more of the total fatty acids.

[0016] (In the general formula (1), R1 is selected from hydrogen or any fatty acid, R2 is selected from hydrogen or any fatty acid, at least one of R1 or R2 is a fatty acid, and X is one selected from a choline residue (PC), an ethanolamine residue (PE), an inositol residue (PI), or a serine residue (PS).)

[0017] Phospholipids can be analyzed using NMR. Details are given in Oleoscience Vol. 19, No. 2 (2019). In summary, this is a quantitative method using 1H NMR (Accurate quantitative NMR with internal reference substance; AQARI).

[0018] The fatty acid composition of the composition was analyzed by gas chromatography (Shimadzu GC-14B) using a Fused Silica Capillary Column Omegawax 320 (30 m × 0.32 mm id) [Supelco Inc., Bellefonte, PA, USA] with helium as the carrier gas and flame ionization detection (FID).

[0019] Dissolving the yeast extract of the present invention in a specific solvent ensures the stability of the yeast extract components and also allows for the production of a clear composition containing the yeast extract of the present invention, such as for use as a topical agent. The solvent used can be a dihydric or polyhydric alcohol or polyol. More specifically, examples of the solvent include glycerin, propylene glycol, propanediol, 1,3-butylene glycol, 1,3-propanediol, and dipropylene glycol. The term "polyol" as used herein refers to an aliphatic compound having two or more hydroxy groups, or a carbohydrate in which at least some of the carbonyl groups in the molecule have been reduced to hydroxy groups. The polyol used in the present invention can be a commonly available one, and examples thereof include glycerol, erythritol, arabitol, xylitol, mannitol, maltitol, 3-methyl-1,3-butanediol, 2-methyl-1,3-propanediol, trimethylolpropane, pentaerythritol, sorbitol, pentylene glycol, hexylene glycol, 1,2-hexanediol, caprylyl glycol, diglycerin, polyglycerin, diethylene glycol, polyethylene glycol, polypropylene glycol, and reduced starch saccharification products. These solvents can be used alone or in combination of two or more. Among these, pentylene glycol, glycerin, propylene glycol, 1,3-butylene glycol, and dipropylene glycol are preferred, and more preferred are one or more solvents selected from 1,3-butylene glycol, pentylene glycol, and glycerin. Even more preferred is a combination in which pentylene glycol is selected and one or more solvents of 1,3-butylene glycol or glycerin are used.

[0020] In the present invention, yeast extract is dissolved in the solvent used in the first step. While the amount of solvent used is optional, from the perspective of ensuring clarity, it is particularly preferable to dissolve the yeast extract in a solvent that is at least three times the weight of the yeast extract. When using pentylene glycol, dissolve it in at least 3 weights per 1 weight of yeast extract. When combining multiple solvents, additional 1,3-butylene glycol is added to achieve a yeast extract:1,3-butylene glycol weight ratio of 1:0-2. Furthermore, when glycerin is added as a third component, it is added to achieve a yeast extract:glycerin weight ratio of 0-3. Furthermore, the content of yeast extract in the topical composition of the present invention is 10% by weight or more, preferably 15% by weight or more, and particularly preferably 20% by weight or more. From the perspective of ensuring clarity, a higher yeast extract content is more preferable, even when the pentylene glycol and yeast extract ratio is the same.

[0021] In addition, the topical composition of the present invention can be blended with various additives that can be used in topical skin preparations, cosmetics, etc. There are no limitations on the materials that can be used, and the composition may contain various active ingredients such as oils, alcohols, ethers, surfactants, sequestering agents, pearlescent agents, amino acids, organic amines, water-soluble polymers, polymer emulsions, pH adjusters, vitamins, antioxidants, preservatives, water-soluble polymers, fragrances, ultraviolet absorbers, and ultraviolet blockers.

[0022] The topical composition of the present invention prepared as described above can be added to cosmetics, quasi-drugs for skin, topical preparations, etc. for use. The amount of the product of the present invention added is arbitrary, and a blending amount that can be appropriately selected by a person skilled in the art can be adopted depending on the properties of the cosmetic product to be used. Typically, the upper limit is selected from 20% by weight or less, 15% by weight or less, 10% by weight or less, 7.5% by weight or less, 6% by weight or less, or 5% by weight or less, and the lower limit is selected from 0.1% by weight or more, 0.2% by weight or more, 0.3% by weight or more, 0.4% by weight or more, or 0.5% by weight or more. The most common blending amount is 0.5% by weight to 5% by weight.

[0023] The form of the topical preparations, quasi-drugs for skin and cosmetics containing the composition of the present invention is not particularly limited, and examples thereof include basic cosmetics such as lotion, milky lotion, cream, gel, beauty serum, sunscreen cosmetics, pack, hand cream, foot cream, body lotion and body cream; cleansing cosmetics such as face wash, makeup remover, soap, body shampoo, shampoo, rinse, conditioner and nail polish remover; and make-up cosmetics such as foundation, makeup base, lip cream, lipstick, cheek color, eye color, eyebrow pencil and nail polish.

[0024] The topical preparations, quasi-drugs for skin, and cosmetics containing the composition of the present invention may contain a commonly used base or carrier, and, if necessary, various additives. Examples of additives include excipients, oils, powders, buffers, solubilizers, antioxidants, surfactants, thickeners, preservatives, pH adjusters, chelating agents, stabilizers, irritation reducers, antiseptics, pigments, colorants, fragrances, gloss-imparting agents, gelling agents, alcohols, water-soluble polymers, film-forming agents, resins, and keratolytic agents. The bases, carriers, and various additives may be used alone or in combination as necessary.

[0025] Furthermore, topical preparations, quasi-drugs for skin, and cosmetics containing the composition of the present invention may contain active ingredients other than the composition of the present invention, provided that the effects of the composition of the present invention are not impaired. Examples of such active ingredients include moisturizing ingredients, anti-inflammatory ingredients, antibacterial ingredients, cell activating ingredients, anti-aging ingredients, blood circulation promoting ingredients, UV protection ingredients, whitening ingredients, vitamins, proteins, peptides, amino acids, and alcohols. These active ingredients may be used alone or in combination as needed.

[0026] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0027] (Production Example 1 of a Yeast Extract Containing Yeast-Derived Dietary Fiber) A yeast extract was produced using yeast cells ("KR Yeast" from Mitsubishi Corporation Life Sciences Co., Ltd.). 1,000 g of the raw material was extracted with 90% ethanol at 70°C for 7 hours. The extract was then filtered through filter paper (ADVANTEC, No. 5C) to remove insoluble matter. The filtrate was then concentrated to dryness under reduced pressure using a rotary evaporator to obtain 55 g of a dried composition. This dried composition was used in the following study as Production Example 1. When the components of the dried composition were analyzed using the ether extraction method, the lipid content per 100 g was 100 g, indicating that the composition was almost entirely lipid. Fractions were collected by column chromatography, and mass analysis of polar and nonpolar lipids revealed that polar lipids accounted for 91% by mass and nonpolar lipids for 9% by weight.

[0028] The fatty acid composition of the phospholipid produced in Production Example 1 is shown in Table 1 below (gas chromatography (hydrogen flame ionization detector)).

[0029] Next, the solvents described in Production Example 1 were mixed to obtain the blending ratios shown in Table 2, and the clarity was visually confirmed.

[0030]

[0031] In the examples, the yeast extract was clear, and the stability of the components contained in the yeast extract was confirmed.

[0032] 1: Comparative Example 1, 2: Example 1

Claims

1. A topical composition comprising a yeast extract and one or more solvents selected from dihydric alcohols, polyhydric alcohols, and polyols.

2. The topical composition of claim 1, wherein the yeast extract of claim 1 is an organic solvent extract.

3. The topical composition according to claim 1, wherein the dihydric alcohol, polyhydric alcohol or polyol is pentylene glycol, glycerin, propylene glycol, 1,3-butylene glycol or dipropylene glycol.

4. Cosmetics containing the topical compositions of claims 1 to 3 5. An external preparation for skin containing the external composition of claims 1 to 3.

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