New polyphenolic composition

Abstract

A polyphenolic composition with considerable functional properties is described, obtained from beer wort by means of a first precipitation of present proteins and a second extraction in solid phase for the removal of the carbohydrates. The new polyphenolic composition of the present invention can be obtained, mutatis mutandis, through passage of the beer wort through specific resins for the adsorption of the polyphenols and subsequent desorption thereof by elution.

Description

[0001] “NEW POLYPHENOLIC COMPOSITION

[0002] FIELD OF THE ART

[0003] The present invention generally relates to food, nutraceutical, pharmaceutical and cosmetic / dermatological sector and, more specifically, to a new polyphenolic composition with considerable functional properties, obtained from beer wort by means of a solid phase extraction process.

[0004] STATE OF THE ART

[0005] Polyphenols are specific molecules of the plant kingdom. The base structural element is a benzenic ring thereto one or more hydroxyl groups are directly connected. More than 8000 known compounds correspond to this definition. Depending upon their structural features they are divided into several classes, comprising flavonoids, lignans, phenolic acids, stilbenes, other polyphenols and non-phenolic metabolites (http: / / phenol-explorer.eu). Thanks to their biological properties, such as for example antioxidant, bioacid, anti-inflammatory properties, the polyphenols can be of interest in various fields, such as for example food / nutraceutical, cosmetic, phytotherapeutic, veterinary field.

[0006] The polyphenolic compounds currently available on the market derive from various matrices of plant origin such as, olives, coffee, cocoa, tea, grapes, wine, apples, soy, algae, berries such as for example currants and raspberries, but there is limited evidence in the patent literature about sources such as barley malt and hop, which constitute the beer wort.

[0007] In this context, the inventors of the present invention result to be applicants (WO2019038658) of a patent application for industrial invention which relates to a natural polyphenolic composition starting from unfermented beer wort and to a method for the preparation of said composition, which preserves intact most polyphenols present in the raw materials by obtaining a composition characterized by a high antioxidant and antitumour activity.

[0008] However, in the composition obtained with the method of the above-mentioned invention, starting from beer wort, the presence of non-active compounds is still significant, such as for example proteins and glucides, which lower in the mass unit the biological activity of the polyphenolic compounds and limit the biological functionality thereof. Therefore, in the light of the antioxidant and antitumour properties it is desirable to remove these non-active compounds to magnify the effects of the polyphenolic component.

[0009] The document W02009144406 relates to the use of a partially purified beer as starting material to obtain an extract rich in polyphenols derived from beer wort. In the said document, "partially purified beer" means beer obtained after having eliminated yeast, plant residues and colloidal particles, for example by filtration on kieselguhr and / or centrifugation. Obtaining an extract rich in polyphenols comprises a contact phase of the partially purified beer with a resin based on poly[1 -(2-oxo-1 -pyrrolidinyl)]ethylene (PVPP) for the adsorption of polyphenols, their desorption by washing with an alkaline solution for sodium hydroxide, the acidification of said solution by phosphoric acid, a new adsorption of the polyphenols on a second resin such as for example Amberlite® FPX 68, the subsequent new desorption by an organic solvent, the concentration and the final purification of the obtained polyphenols.

[0010] Apart from the fact that the raw material is a partially purified beer, therefore already fermented and as a result of chemical fermentation processes depleted of the characteristic amount of polyphenols naturally present in malt, it is easy to understand that the adsorption, desorption, concentration and final purification cycle could further degrade the chemical difference of the polyphenols naturally present in malt, by compromising the antioxidant and antitumor activity of the extracts, and also raising production costs.

[0011] Due to these drawbacks, then it is still felt the need for having available a new composition enriched with polyphenols coming from beer wort, said composition being in the liquid state and in the solid state, preserving the quality of the naturally present ingredients and capable of guaranteeing the same performance or higher performance than the formulations available on the market.

[0012] The main object of the present invention is therefore to overcome the above-illustrated technical drawbacks, by providing a new composition concentrated in polyphenols by removing water, the proteins and the carbohydrates naturally present as major components in the unfermented beer wort, by preserving consequently the quality and quantity of the polyphenols subject of the invention.

[0013] SUMMARY OF THE INVENTION

[0014] This object is achieved with the present invention, which provides a new polyphenolic composition through an enrichment method comprising in a first aspect the following steps: a) eluting the polyphenolic composition deriving from unfermented beer wort and / or maltine and / or malt and hop extract comprising water, proteins, carbohydrates and polyphenolic components obtained by means of the process described in WO2019038658 by a suitable solid phase, specific for the adsorption of polyphenols or non-active compounds; b) recovering from step a) a new composition concentrated in polyphenols.

[0015] Optionally, the present invention also provides a new polyphenolic composition obtained through a method wherein the raw material to be treated at step a) is a powder dispersed in water.

[0016] Other non-limiting embodiments of the present invention, more specifically the steps through which it can articulate, are illustrated in the description shown below.

[0017] BRIEF DESCRIPTION OF FIGURES

[0018] Figure 1 represents a comparison between the viability of HL-60 tumour cells treated with the polyphenolic composition obtained through the process described in WO201 9038658 and the viability of the same tumour cells treated with the new composition of the present invention.

[0019] Figure 2 represents the absence of toxicity in differentiated HL-60 cells, miming a nontumour cell phenotype, treated with the composition of the present invention.

[0020] Figure 3 represents the antioxidant action on a cell line of human keratinocytes for evaluating the effectiveness for cosmetic use of the composition of the present invention.

[0021] DETAILED DESCRIPTION OF THE INVENTION

[0022] The present invention describes a new polyphenolic composition obtained by means of an enrichment process comprising the following steps: a) eluting through a suitable solid phase, specific for the adsorption of polyphenols or non-active compounds, a polyphenolic composition deriving from unfermented beer wort and / or maltine and / or malt and hop extract comprising water, proteins, carbohydrates and polyphenolic components having the ratios compared to the caffeic acid shown in the following Table 1 ; b) recovering from step a) a new composition concentrated in polyphenols, characterized in that said new composition has a synergistic behaviour among the polyphenolic compounds. The polyphenolic composition obtained through the process described in WO201 9038658 comprising water, proteins, carbohydrates and polyphenolic components has a content of polyphenols as substantially represented in Table 1 and it further comprises on dry base approximately 87% of carbohydrates and approximately 6% of proteins.

[0023] Table 1

[0024] Said polyphenolic composition used in step a) in an additional embodiment is a powder dispersed in water. The term “synergistic behavior” refers to a mixture of components, such as those described in the present disclosure, when the resulting effect, such as a therapeutic effect or an adjuvant effect to a therapy, is greater than the expected sum of the effects of the individual components when taken separately. A therapeutic effect is not necessarily a cure and may, for example, consist of symptom relief or a general improvement in condition

[0025] The terms “comprises”, “comprising” as used in the present description before a series of components, features, elements, does not exclude or precludes the presence of other components, features, elements according to the techniques known to the person skilled in the art.

[0026] The term “dispersion” used in the present description is meant in broader sense as mixture comprising, for example, suspensions and solutions.

[0027] The term “beer wort” used in the present description relates to the sugary liquid obtained from barley malt after the phases of milling, mashing, filtering of spent grains, boiling and hopping.

[0028] All other terms used in the description of the present invention, unless otherwise specified, have to be interpreted according to their ordinary meaning known to the person skilled in the art.

[0029] In a first aspect, the present invention relates to a new polyphenolic composition obtained through the above-illustrated steps a) and b).

[0030] Optionally, the present invention even provides a new polyphenolic composition obtained through a method in which the raw material to be treated at step a) is an aqueous dispersion obtained by dispersing in water the powder which is obtained by subjecting to freeze-drying and / or spray drying the polyphenolic composition obtained from the process described in WO2019038658 and having the following ratios between polyphenolic components compared to caffeic acid set equal to 1 : Caffeic Acid 1

[0031] Chlorogenic Acid 0.83 - 1.01

[0032] M-coumaric Acid 1.04 - 1.27

[0033] P-hydroxybenzoic Acid 1.13 - 1.39

[0034] Sinapic Acid 1.33 - 1.63

[0035] Epicatechin 1.67 - 2.04

[0036] Protocatechuic Acid 1.73 - 2.11

[0037] Catechin 2.39 - 2.93

[0038] P-coumaric Acid 5.13 - 6.27

[0039] Ferulic Acid 8.46 - 10.3

[0040] Vanillic Acid 2.47 - 3.01

[0041] In a preferred embodiment, said aqueous dispersion is a solution with a solute having concentration comprised between 110 and 130 mg / ml.

[0042] Still optionally, the present invention also provides a polyphenolic composition obtained through a method wherein the raw material to be treated at the aboveillustrated step a) is a liquid composition comprising beer wort and / or maltine and / or malt and hop extract.

[0043] In an additional preferred embodiment, the solid phase of step a) is used to remove carbohydrates and it is the one commonly used in reverse phase extraction techniques, such as for example high hydrophobicity silica in which part of silanol groups is functionalized with C18 hydrocarbon chains. The diameter of silica particles is comprised between 55 and 105 pm, with a porosity comprised between 100 and 130 A. Preferably, in this embodiment it is appropriate to pretreat the polyphenolic composition obtained through the process described in WO2019038658 with a strong acid for the separation of proteins before the elution phase. The used strong acid is selected from the group comprising the trifluoroacetic acid (TFA) and the trichloroacetic acid (TCA). Still more preferably, the used strong acid is the trichloroacetic acid with a final concentration in the solution comprised between 10% w / w and 15% w / w. Optionally, after the treatment with strong acid an additional digestion stage at low temperature, preferably comprised between 0°C and 5°C, can be comprised. The removal at step of the precipitated proteins can take place through any one of the methods known to the person skilled in the art, for example through centrifugation and / or filtration.

[0044] The elution can take place by hydro-alcoholic and / or hydro-organic solution. In a preferred embodiment, said elution takes place by hydro-organic solution, wherein the organic solvent mixable with water is acetonitrile. By modulating the amount of acetonitrile (10%, 35%, 70% w / w) according to the methods commonly known to the person skilled in the art, it is possible to recover several fractions of polyphenolic compounds having different antioxidant activity. In an additional preferred embodiment, the acetonitrile is present with a ratio of 35% w / w with respect to the total eluent mass.

[0045] Surprisingly, the new polyphenolic composition eluted with 35% w / w of acetonitrile resulted to be the one with the highest antioxidant activity and, at the same time, the only one having a cytotoxic activity on the HL-60 tumour cells since it reduces the cellular viability of about 80%, the concentration of 0.78 mg / ml being equal, as substantially shown in Figure 1 (p<0.001 t-test), by giving undirect evidence of the synergistic behaviour of the polyphenolic compounds of the present invention, improved by the removal of the non-active fraction represented by proteins and carbohydrates. Moreover, said polyphenolic composition eluted with 35% w / w of acetonitrile is not cytotoxic on the same differentiated HL-60 cells which can represent a model of normal cells, as substantially shown in Figure 2. At last, the same bioactive fraction F35% resulted to have a significative effect in the reduction of the intracellular concentration of ROS (“Reactive Oxygen Species”, considered cause of skin aging and common markers of various dermatological pathologies) on a cell line of human keratinocytes (HaCaT), as substantially represented in Figure 3. The HaCaT cells are keratinocytes derived from adult human skin and spontaneously immortalized, constituting an experimental model widely used in the protocols of the scientific research for the evaluation of the effectiveness of chemical substances for cosmetic use.

[0046] The person skilled in the art will agree that, in order to recover amounts compatible with an industrial application and not only at level of laboratory or pilot preparations, the new composition of the present invention can be obtained by the polyphenolic composition obtained from the process described in WO2019038658 by industrial systems, both in continuous and discontinuous mode, by developing the abovedescribed step a) and step b) through methods already consolidated for other processes, such as for example adsorption on specific membranes, by means of a process comprising the following steps:

[0047] 1 ) passage of the polyphenolic composition obtained from the process described in WO2019038658 through specific selective membranes for the adsorption of polyphenols;

[0048] 2) desorption of polyphenols by elution with suitable eluent, said eluent preferably being a hydro-organic mixture;

[0049] 3) recovery of the liquid composition obtained at step 2) concentrated in polyphenols. In all above-mentioned processes, the removal of the organic part in the final eluate, aimed at obtaining a predominantly aqueous composition that complies with regulatory limits for residual solvents, can take place through one of the techniques known to the person skilled in the art, such as for example concentration and / or distillation. Optionally, after all above-described processes, the recovery in solid form of the eluted polyphenolic compounds can be provided, through any one of the methods known to the person skilled in the art, for example by freeze-drying and / or spray-drying.

[0050] The advantage of the present invention with respect to other ones present in the state of art is the capability of preserving the quality and quantity of the polyphenolic profile present in the raw material, by enriching the content and by guaranteeing significantly lower dosages with respect to the formulations already available on the market.

[0051] The person skilled in the art will further appreciated that, apart from the polyphenolic composition obtained through the process described in WO2019038658 and from the beer wort, functional polyphenolic compositions according to the method of the present invention can be obtained from other matrices such as for example maltine, malt and hop extract, non-alcoholic beer.

[0052] In another aspect of the present invention, the obtained polyphenolic composition can be advantageously used for the preparation of solid dosage forms such as tablets, capsules, sachet, granulates, powders, suppository drugs.

[0053] As it is known to the person skilled in the art, in the solid dosage forms additional excipients and ingredients are added to complete and stabilize the formulation. The excipients are selected from the group comprising for example fillers, binders, disintegrants, lubricants, thickeners, flavours. Other additional ingredients which can be included in the composition are known in the art and described, for example, in Gennaro A.R. et al., Remington’s Pharmaceutical Sciences, Mack Publishing Co., 1985, Easton, Pa., 17th edition.

[0054] The fillers can be selected from the group comprising for example microcrystalline cellulose, lactose monohydrate, calcium hydrogen phosphate, sorbitol, starch, modified starches, talc, kaolin, bentonite, montmorillonite, calcium carbonate, magnesium carbonate, calcium silicate, aluminium hydroxide, amorphous silica or mixtures of these substances.

[0055] The binders can be selected from the group comprising for example gelatine, lactose monohydrate, sorbitol, sucrose, xylitol, maltitol, starch, modified starches, methylcellulose, 2-hydroxyethylcellulose, 2-hydroxypropylcellulose, sodium carboxymethylcellulose, polyethylene glycols, polyglycerols, polyvinylpyrrolidone, copolymers of polyvinylpyrrolidone, carrageenans, or mixtures of these substances. The disintegrants can be selected from the group comprising for example starch, modified starches, sodium starch glycolate, methylcellulose, sodium carboxymethylcellulose, 2-hydroxyethylcellulose, 2-hydroxypropylcellulose, polyvinylpyrrolidone, copolymers of polyvinylpyrrolidone, or mixtures of these substances.

[0056] The lubricants can be selected from the group comprising for example magnesium stearate, calcium stearate, zinc stearate, stearic acid, talc, or mixtures of these substances.

[0057] The thickeners can be selected from the group comprising for example polyacrylic acid, copolymers thereof, or salts thereof of sodium, potassium or triethanolamine, methylcellulose, sodium carboxymethylcellulose, 2-hydroxyethylcellulose, 2- hydroxypropylcellulose, maltodextrin, starch, modified starches, rice flour, polyglycerols, polyethylene glycols, gelatine, pectin, agar agar, carrageenans, gum arabic, alginic acid, sodium alginate, or mixtures of these substances. In an additional aspect of the present invention, the obtained polyphenolic composition can be advantageously used for preparing liquid dosage forms such as, for example, drinks, syrups, drops, eye drops.

[0058] As it is known to the person skilled in the art, in the liquid dosage forms additional excipients and ingredients are added to complete and stabilize the formulation. The excipients are selected from the group comprising for example surfactants, emulsifies, humectants, dispersants, diluents, binders such as for example xylitol, stabilizers such as gum arabic, rheological modifiers such as xanthan gum, disintegrants, lubricants, thickeners such as for example guar rubber, sweeteners such as for example steviol glycosides, flavours, preservatives.

[0059] Examples of preservatives can be tocopherol, tocopherol acetate or ascorbic acid. Flavours can be for example mint, blueberry, strawberry, raspberry, vanilla, lemon or mixtures thereof.

[0060] Other additional ingredients which can be included in the composition are known in the art and described, for example, in Gennaro A.R. et al., Remington’s Pharmaceutical Sciences, Mack Publishing Co., 1985, Easton, Pa., 17th edition.

[0061] In order to preserve the quality of ingredients, it is preferable that the mixing of the components for the preparation of the liquid dosage forms is performed at a temperature lower than 25°C, preferably in the range 10°C - 15°C.

[0062] In another aspect of the present invention, the obtained polyphenolic composition can be advantageously used for preparing a semi-solid dosage forms such as, for example gels, ointments, lubricants, pastes, creams. The formulation is produced by means of common processes known to those skilled in the art of the pharmaceutical technology [Gad S.C. et al, Manuale di Produzione Farmaceutica: Produzione e Processi, 2008, Wiley], As the person skilled in the art could appreciate, the composition of the present invention, in the suitable dosage forms, can be advantageously administered as functional food, functional drink (in the form of, for example, fruit juices, soft drinks, energy drinks) dietary supplement and / or pharmaceutical formulations for use in animal or human subjects for the treatment of chronic-degenerative diseases, including but not limited to cardiovascular, neurodegenerative, autoimmune, gastrointestinal, metabolic, and oncological disorders. Moreover, as recently highlighted by scientific publications [see for example Di Salvo E. et al., Polyphenols from Mediterranean Plants: Biological Activities for Skin Photoprotection in Atopic Dermatitis, Psoriasis, and Chronic Urticaria, Plants, 2023,15; 12(20):3579], the polyphenolic composition of the present invention can be advantageously used in the cosmetic and cosmeceutical field, in the suitable dosage forms for topical use, for the treatment of skin pathologies such as for example dermatitis and / or psoriasis. To this regard, the person skilled in the art will further agree, based also on the inventors’ preliminary investigations conducted on HaCaT cells, that the composition of the present invention can be advantageously used, in the suitable dosage forms such as for example creams, lotions, solutions and make-up products, for the skin anti-aging treatment or generally for the relief of skin disorders related to aging.

[0063] A wide and qualified scientific literature suggests that several classes of polyphenols, apart from the known antioxidant and anticancer properties, also exhibit antibacterial (against both Gram-positive and Gram-negative bacteria), antiviral and antifungal activities. In many instances, the mechanisms of action and structure-activity relationships have been elucidated [see for example Daglia M., Polyphenols as Antimicrobial Agents, Curr. Opin. Biotechnol., 2012, 23(2), 174-181 ], More specifically, there is evidence in literature showing that some tea-derived proanthocyanidins exhibit a powerful antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) infection, a widely spread and endemic porcine pathogen that causes significant economic losses for the global porcine industry (see for example Zhang M et al., Inhibition of Proanthocyanidin A2 on Porcine Reproductive and Respiratory Syndrome Virus Replication In Vitro, Pios One, 2018, 13(2), e0193309). This suggests the potential use of the polyphenolic composition of the present invention, in the suitable dosage forms, for the treatment of pathologies due to infectious agents in the human being and animals (such as for example AIDS, tuberculosis, gonorrhoea, malaria, flu, chronic infections caused by bacterial biofilms), alone or in synergistic combination with the traditional treatments based on synthetic antibiotics.

[0064] Similarly, some proanthocyanidinic compounds showed promising antimicrobial activities against food-borne bacteria [see for example Cobo A. et al., Synthesis of Analogs to A-Type Proanthocyanidin Natural Products with Enhanced Antimicrobial Properties against Foodborne Microorganisms, Molecules, 2023, 28(12), 4844],

[0065] The person skilled in the art then will agree that the new polyphenolic composition of the present invention, being reasonably concentrated in proanthocyanidins as consequence of the removal of the non-active components, may be advantageously employed as a preservative owing to its antioxidant, antimicrobial and antibiofilm potential against foodborne bacteria.

[0066] The present invention is illustrated in greater detail, without limitation, by the following examples.

[0067] EXAMPLE 1 : removal of proteins.

[0068] A portion of the polyphenolic composition obtained through the process described in WO201 9038658 in powder form was brought in solution with bi-distilled water at a concentration of 125 mg / ml. Then, 50 ml of this solution were treated with trichloroacetic acid (final concentration of TCA = 12% w / w) and incubated at 0°C for one hour. The precipitate was removed by centrifugation and the supernatant liquid used for the subsequent purification phase.

[0069] EXAMPLE 2: final purification.

[0070] 40 ml of solution obtained according to Example 1 (about 5000 mg) were loaded on a Sep-Pak C18 unit (catalogue number WAT023635; Waters SpA, Sesto San Giovanni, Ml, Italy). The bioactive fractions (F10%, F35%, F70%) were then eluted by using 10%, 35% and 70% w / w, respectively, of acetonitrile in water.

[0071] EXAMPLE 3: biological activity of the bioactive fraction F35%.

[0072] In order to compare the biological activity of the polyphenolic composition obtained through the process described in WO2019038658 with that of the F35% polyphenolic fraction and the other eluted fractions, the antiproliferative effect was evaluated on a cell line derived from human promyelocytic leukemia, namely HL-60. The cells, seeded at a density of 2.5x10'5 cells / ml in 48-well plates, were incubated for 24 hours with the polyphenolic composition obtained through the process described in WO2019038658 and with the polyphenolic fractions eluted with 10%, 35% and 70% of acetonitrile at a final concentration of 0.78 mg / ml. At the end of incubation, in order to quantify the number of living cells, the CyQuant assay (Thermo Fischer Scientific, Rodano, Ml, Italy) was performed. This assay employs a nuclear dye that selectively binds to nucleic acids, emitting fluorescence proportional to the number of viable cells, thereby enabling accurate quantification of living cells. CyQuant was added to the culture medium (diluted 1 :10) and incubated for one hour at 37°C. The fluorescence was then measured with a reader of plates (Synergy HT Biotek, Milan, Italy) by using as excitation and emission filters 485 nm and 530 nm, respectively. The results were expressed as fluorescence percentage with respect to the control cells not treated, with the polyphenolic composition obtained through the process described in WO201 9038658, and with the polyphenolic fractions, as substantially represented in Figure 1 .

[0073] EXAMPLE 4: absence of cytotoxycity of the bioactive fraction F35%.

[0074] In order to verify the absence of cytotoxicity of F35% fraction in “not malignant” cells, HL-60 cells were induced to differentiate in order to reproduce the cell phenotype of normal macrophages. The differentiation was induced by stimulating the cells with PMA (phorbol-12-myristate-13-acetate, 20 pM) for 72 hours. At the end of the differentiation, the cells were stimulated for 24 hours with 0.8 at 1.6 mg / ml of F35%. The viability assay performed with CyQuant method did not evidence any significant change in cell viability, as substantially represented in Figure 2.

[0075] EXAMPLE 5: reduction of ROS (Reactive Oxygen Species) by the the bioactive fraction F35%.

[0076] In order to verify the potential cosmeceutical role of the F35% polyphenolic fraction, its capability of reducing the concentration of Reactive Oxygen Species (ROS) in human keratinocytes (HaCaT cell line) was evaluated. To assess intracellular oxidative stress, HaCaT cells were seeded at a density of 1x1(75 cells / ml in 96-well plates and incubated for 30 minutes with 10 pM of the fluorescent probe 2’,7’-dichlorofluorescein diacetate (DCFH-DA; Thermo Fischer Scientific), a probe sensitive to ROS Following probe loading, cells were stimulated with 0.2 mg / ml of F35% fraction. After one hour of incubation, the fluorescence was then measured with a plate reader (Synergy HT Biotek), with excitation and emission wavelengths set at 485 nm and 530 nm, respectively. The fluorescence intensity was expressed as a percentage relative to the untreated control cells, as substantially represented in Figure 3.

Claims

CLAIMS1 . A new polyphenolic composition obtained by means of an enrichment process comprising the following steps: a) eluting through a suitable solid phase, specific for the adsorption of polyphenols or non-active compounds, a composition obtained from a powder deriving from unfermented beer wort and / or maltine and / or malt and hop extract dispersed in water, said composition to be eluted comprising water, proteins, carbohydrates and polyphenolic components having the following ratios compared to caffeic acid set equal to 1 :Caffeic acid 1Chlorogenic Acid 0.83 - 1.01M-coumaric Acid 1.04 - 1.27P-hydroxybenzoic Acid 1.13 - 1.39Sinapic Acid 1.33 - 1.63Epicatechin 1.67 - 2.04Protocatechuic Acid 1.73 - 2.11Catechin 2.39 - 2.93P-coumaric Acid 5.13 - 6.27Ferulic Acid 8.46 - 10.3Vanillic Acid 2.47 - 3.01 b) recovering from step a) a new composition concentrated in polyphenols, characterized in that said new composition has a synergistic behaviouramong the polyphenolic compounds.

2. The new composition according to claim 1 , wherein the solid phase at step a) comprises high hydrophobicity silica wherein part of silanol groups is functionalized with C18 hydrocarbon chains, with the particle diameter of said silica comprised between 55 and 105 pm, and with a porosity between 100 and 130 A.

3. The new composition according to claim 2, wherein the raw material of step a) is treated with a strong acid, said strong acid being selected from the group comprising trifluoroacetic acid and trichloroacetic acid.

4. The new composition according to claim 2, wherein the elution occurs by means of a hydro-alcoholic and / or hydro-organic solution.

5. The new composition according to claim 4, wherein the organic solvent is acetonitrile present with a ratio of 35% w / w with respect to the total eluent mass.

6. The new composition according to claim 1 ), wherein the solid phase of step a) comprises specific membranes for the adsorption of polyphenols.

7. The new composition according to claim 6, wherein said specific membranes for the adsorption of polyphenols comprise co-polymers of polyvinylpyrrolidone.

8. The new composition according to claim 1 , characterized in that saidcomposition is used for the preparation of solid dosage forms such as tablets, capsules, sachet, granulates, powders, suppository drugs.

9. The new composition according to claim 1 , characterized in that said composition is used for the preparation of liquid dosage forms such as beverages, syrups, drops.

10. The new composition according to claim 1 , characterized in that said composition is used for the preparation of semi-solid dosage forms such as gels, ointments, lubricants, pastes, creams.11 . The new composition according to any one of the preceding claims 1 to 10 for use in the treatment of pathologies of chronic and degenerative type in cardiovascular, neurodegenerative, autoimmune, metabolic, tumour field.

12. The new composition according to any one of the preceding claims 1 to 10 for use in the treatment of skin diseases comprising dermatitis and psoriasis.

13. The new composition according to any one of the preceding claims 1 to 10 for use in the anti-aging skin treatment.

14. The new composition according to any one of the preceding claims 1 to 10 for use in the treatment of diseases by infectious agents deriving from pathogens comprising virus, bacteria, fungi.

15. The new composition according to any one of the preceding claims 1 to 10 for use as antioxidant, antimicrobial and antibacterial agent for food preservation.5

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