Method for using double-cannula continuous irrigation and negative-pressure suction liquid culture medium kit

Abstract

Provided is a method for using a double-cannula continuous irrigation and negative-pressure suction liquid culture medium kit, which comprises preparation work, collection of a drainage solution of an aerobic culture flask, and collection of a drainage solution of an anaerobic culture flask. The preparation work is as follows: checking patient information and preparing negative pressure tools. The collection of a drainage solution of an aerobic culture flask is as follows: directly collecting the drainage solution using a negative pressure. The collection of a drainage solution of an anaerobic culture flask is as follows: first, collecting the drainage solution using a negative pressure, and then removing air in the drainage solution using an air removal assembly. A culture flask placement box is used for storing a culture flask containing an aerobic culture solution and a culture flask containing an anaerobic culture solution. The two culture flasks are combined and placed in one box, and the two culture flasks can be separately opened for direct use during sample collection, such that the operation is simple and convenient; a negative pressure connector and a liquid inlet connector are used for being directly connected to a negative pressure pipe and double cannulas, achieving negative-pressure collection of the drainage solution; extraction can be completed in one go, and operation can be completed by a single person, thereby preventing medical personnel from contacting the drainage solution while preventing the drainage solution from contacting the outside. Therefore, occupational exposure is avoided, and secondary pollution is also avoided.

Description

Method for using double cannula continuous flushing negative pressure suction liquid culture medium set TECHNICAL FIELD

[0001] The present application relates to a method for using a double cannula continuous flushing negative pressure suction liquid culture medium set. BACKGROUND

[0002] Infectious pancreatic necrosis (IPN) belongs to deep soft tissue abscess, which is also known as pancreatic abscess. At present, with the release of medical evidence, the minimally invasive "step-up" method has become the preferred treatment strategy for IPN. The double cannula continuous flushing negative pressure suction is the second step of the "step-up" method. IPN patients often have repeated high fever, so it is necessary to find the cause of fever while cooling down. Blood culture and drainage liquid culture (aerobic and anaerobic) are often used to find pathogenic bacteria. The use of antibiotics for the drug resistance of bacteria in blood and drainage liquid is particularly important for the control of the patient's condition.

[0003] The front end of the double cannula is generally placed in one or more abscess cavities, and the pipeline is exposed for a long time. When the double cannula is indwelled and the drainage liquid is taken for culture, the following two methods are often used in clinic:

[0004] 1. Directly aspirate from the end of the pipeline with a syringe, but because of the pressure problem, it is difficult to aspirate the liquid or the amount of the aspirated liquid is far from the standard. Repeatedly aspirating not only causes local discomfort of the patient but also easily causes contamination.

[0005] 2. The pus sucked out by the double cannula continuous flushing negative pressure suction is stored in a pouch. When the specimen is taken, the nurse will help the doctor to suspend the flushing, separate the pipeline from the pouch in the suction bottle, pour the solution out from the entrance of the pouch, and pour it into a sterile cup. Then the pus is aspirated from the sterile cup and injected into the blood oxygen and anaerobic blood culture bottles. After the specimen is taken, the pouch is inserted back into the drainage bottle or a new pouch is directly replaced. In this process, the operator may be splashed by the pus if he is not careful, causing physical and mental discomfort. At the same time, it also wastes time and human resources, increases the cost of the patient, and the solution poured out by the pouch is exposed for a long time, which cannot be ruled out the possibility of secondary contamination.

[0006] In Chinese patent: 201620258972.5, named: a VSD negative pressure suction device capable of instant sampling and detection, which is recorded as follows: the drainage channel includes at least one double cannula, the double cannula has a drainage section and a negative pressure section; a plurality of drainage holes are formed in the wall of the drainage section, the VSD dressing wraps the drainage section, and the negative pressure section is connected to a negative pressure source.

[0007] The double-casing pipeline is provided with at least one sampling tee close to the negative pressure section, one end of the sampling tee is communicated with the drainage section, one end is communicated with the negative pressure section, and the other end is communicated with a sampling interface; the sampling interface is sealingly connected with a chromogenic culture dish and / or a liquid storage bottle.

[0008] As shown in the drawings in the patent: 201620258972.5, the negative pressure suction device mainly utilizes the negative pressure drainage device capable of instant sampling detection to perform instant detection on physiological parameters of wound exudates. The device mainly utilizes a tee valve to control suction of drainage liquid. This structure causes air to enter the culture dish when the drainage liquid is sucked into the culture dish. Therefore, this structure is only suitable for aerobic culture liquid and is not suitable for anaerobic culture liquid. SUMMARY

[0009] The technical problem to be solved by the present application is that the existing double-casing continuous flushing negative pressure suction drainage liquid sample taking is inconvenient, needs multiple operations, and is prone to exposure and secondary pollution.

[0010] In order to solve the above technical problems, a use method of a double-casing continuous flushing negative pressure suction liquid culture medium set is provided; the use method is realized through the following technical scheme: a use method of a double-casing continuous flushing negative pressure suction liquid culture medium set, comprising the following steps:

[0011] Step one: preparation: prepare the culture medium set to be used, and confirm and fill in the patient information, paste the patient information on the side walls of the aerobic culture bottle and the anaerobic culture bottle, and prepare the negative pressure tube and the negative pressure gauge for negative pressure suction;

[0012] Step two: aerobic culture bottle drainage liquid collection:

[0013] S21, take out the aerobic culture bottle from the culture bottle placing box, and then take off the negative pressure connector and the liquid inlet connector on the aerobic bottle cap;

[0014] S22, install the negative pressure tube connected with the negative pressure gauge to the negative pressure connector, and connect the double-casing tube connected with the patient to the liquid inlet connector;

[0015] S23, turn on the negative pressure gauge, the negative pressure gauge provides negative pressure suction, the drainage liquid in the double-casing tube is sucked into the bottle body in the aerobic culture bottle through the liquid inlet connector, and the amount of the sucked drainage liquid is observed through the scale on the bottle body, when the amount is enough, turn off the negative pressure gauge;

[0016] S24, take off the negative pressure tube on the negative pressure connector and the double-casing tube on the liquid inlet connector, and then cover the sealing cover on the negative pressure connector and the liquid inlet connector again;

[0017] S25, the aerobic culture bottle drainage collection is completed, the aerobic culture bottle is placed in one of the culture bottle placement cavities in the culture bottle placement box;

[0018] Step three: anaerobic culture bottle drainage collection:

[0019] S31, the anaerobic culture bottle is taken from the culture bottle placement box, and then the negative pressure connector and the liquid inlet connector on the anaerobic bottle cap are removed;

[0020] S32, the negative pressure pipe connected with the negative pressure table is installed on the negative pressure connector, and the double cannula connected with the patient is connected with the liquid inlet connector;

[0021] S33, open the negative pressure table, the negative pressure table provides negative pressure suction, the drainage in the double cannula is sucked into the injection pipe in the injection assembly through the liquid inlet connector, the drainage accumulates at the bottom of the injection pipe, and the air in the injection pipe is discharged through the exhaust port as the drainage is sucked into the injection pipe, until the injection pipe is filled with drainage or the drainage in the injection pipe reaches a reasonable value, the negative pressure table is closed, and the negative pressure suction is stopped;

[0022] S34, the injection pipe is injected into the bottle body of the anaerobic culture bottle, and when the injection pipe is filled with drainage without air, step S35 is entered, and when the injection pipe has air, step S36 is entered;

[0023] S35, the injection push rod in the injection assembly is pushed to inject the drainage into the bottle body of the anaerobic culture bottle, and the injection amount of the drainage is observed through the scale;

[0024] S36, the injection push rod in the injection assembly is pushed to use the air removal assembly to discharge the air in the injection pipe, and then the injection push rod is pushed to inject the drainage into the bottle body of the anaerobic culture bottle, and the injection amount of the drainage is observed through the scale;

[0025] S37, the negative pressure pipe on the negative pressure connector and the double cannula on the liquid inlet connector are removed, and then the cover is re-covered on the negative pressure connector and the liquid inlet connector;

[0026] S38, the anaerobic culture bottle drainage collection is completed, the anaerobic culture bottle is placed in another culture bottle placement cavity in the culture bottle placement box;

[0027] Step four: after the aerobic culture bottle and the anaerobic culture bottle drainage collection is completed, the collection information is filled on the culture bottle placement box, and the collected aerobic culture bottle and anaerobic culture bottle are sent to culture.

[0028] In the preferred technical solution of the present application, in the S33 step of the third step, the injection tube is communicated with the air removal assembly, and the overflow drainage liquid in the injection tube is stored in the overflow liquid storage bin in the air removal assembly, which facilitates the discharge of air in the injection tube, so that air cannot enter the anaerobic culture bottle when the drainage liquid is injected into the anaerobic culture bottle, and the accuracy of anaerobic culture is ensured.

[0029] In the preferred technical solution of the present application, in the S35 and S36 steps of the third step, the injection push rod is connected with the push rod limiting assembly, the push rod limiting assembly limits the injection of the injection push rod, and the push rod limiting assembly facilitates the control of the injection assembly to inject the drainage liquid into the anaerobic culture bottle, and is convenient to use.

[0030] In the preferred technical solution of the present application, the culture medium set includes an aerobic culture bottle, an anaerobic culture bottle and a culture bottle placing box, the aerobic culture bottle and the anaerobic culture bottle include a bottle body and a bottle cap, the bottle cap is screwed on the bottle body to close the bottle body, and the aerobic culture bottle storing aerobic culture liquid and the anaerobic culture bottle storing anaerobic culture liquid can be detachably placed in the culture bottle placing box.

[0031] In the preferred technical solution of the present application, the anaerobic culture bottle includes an air removal assembly and an injection assembly, the injection assembly is connected with the liquid inlet connector on the anaerobic culture bottle, the air removal assembly is connected with the negative pressure connector on the anaerobic culture bottle, the air removal assembly is communicated with the injection assembly, the air removal assembly discharges the air sucked into the injection assembly, the injection assembly injects the air-free suction liquid into the bottle body storing the anaerobic culture liquid, and the air removal assembly and the injection assembly facilitate the injection of the air-free drainage liquid into the bottle body of the anaerobic culture bottle, prevent air from entering the anaerobic culture bottle, affect the anaerobic culture of the drainage liquid, and ensure the culture effect.

[0032] In the preferred technical solution of the present application, the bottle body is made of transparent material, and a scale for observing the amount of liquid in the bottle body is arranged on the side wall of the bottle body, which facilitates the observation of the amount of drainage liquid in the culture bottle and is convenient to use.

[0033] In the preferred technical solution of the present application, the culture bottle placing box includes a partition plate and an observation window, the partition plate is arranged in the culture bottle placing box to divide the culture bottle placing box into two culture bottle placing cavities, and the observation window is arranged on the two sides of the culture bottle placing box to observe the amount of liquid in the bottle body through the observation window.

[0034] Compared with the prior art, the present application has the following beneficial effects:

[0035] The technical solution of the present invention uses a culture bottle placement box to store culture bottles containing two kinds of culture media, aerobic and anaerobic. Two culture bottles are combined and placed in one box. When sampling, they can be directly opened and used separately, meeting the clinical needs, with simple operation. Also, a negative pressure connector and a liquid inlet connector are directly connected to a negative pressure tube and a double-tube to achieve negative pressure sampling of the drainage fluid. The extraction can be completed once, avoiding repeated extraction, with convenient operation and can be operated by a single person. It avoids the contact between medical staff and the drainage fluid, and at the same time, the drainage fluid does not contact the outside world, preventing occupational exposure and secondary pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] FIG. 1 is a three-dimensional schematic diagram of the present invention;

[0037] FIG. 2 is a three-dimensional schematic diagram of an aerobic culture bottle;

[0038] FIG. 3 is an exploded view of an aerobic culture bottle;

[0039] FIG. 4 is a partial cross-sectional view of an aerobic culture bottle;

[0040] FIG. 5 is an enlarged view of part A in FIG. 4;

[0041] FIG. 6 is a three-dimensional schematic diagram of an anaerobic culture bottle;

[0042] FIG. 7 is a three-dimensional schematic diagram of an air removal component and an injection component;

[0043] FIG. 8 is a partial cross-sectional view of an air removal component and an injection component;

[0044] FIG. 9 is an enlarged view of part B in FIG. 8;

[0045] FIG. 10 is an enlarged view of part C in FIG. (8);

[0046] FIG. 11 is a three-dimensional schematic diagram of a push rod limit component;

[0047] FIG. 12 is a partial cross-sectional view of an anaerobic culture bottle;

[0048] FIG. 13 is a three-dimensional schematic diagram of a culture bottle placement box;

[0049] Explanation of reference signs: 1-oxygen culture bottle, 11-bottle body, 12-oxygen bottle cap, 13-scale, 14-hand support, 15-screw interface, 16-negative pressure connector, 17-liquid inlet connector, 18-sealing gasket, 19-closure, 2-elastic sealing assembly, 21-mounting rod, 22-sealing cone, 23-connecting rod, 24-first spring, 25-spring mounting block, 3-anaerobic culture bottle, 31-anaerobic bottle cap, 32-functional hole, 4-air removal assembly, 41-overflow bin, 42-functional bin, 43-exhaust pipe, 5-injection assembly, 501-injection tube, 502-injection push rod, 503-exhaust port sealing rod, 504-liquid inlet, 505-injection port, 506-first exhaust port, 507-injection piston, 508-second exhaust port, 509-sealing head, 510-second spring, 511-limiting ring, 512-liquid inlet tube, 513-sealing sheet, 6-culture bottle placing box, 61-dividing plate, 62-observation window, 63-culture bottle placing cavity, 7-push rod limiting assembly, 71-limiting rod, 72-limiting groove, 73-limiting clamp. DETAILED DESCRIPTION

[0050] The technical solutions in the embodiments of the present application will be described in detail below with reference to Figs. 1-13 of the drawings. EMBODIMENT

[0051] As shown in Fig. 1, a double-cannula continuous flushing negative pressure suction liquid culture set includes an oxygen culture bottle 1, an anaerobic culture bottle 3 and a culture bottle placing box 6. The oxygen culture bottle 1 for storing oxygen culture liquid is placed in the culture bottle placing box 6, and the anaerobic culture bottle 3 for storing anaerobic culture liquid is placed in the culture bottle placing box 6.

[0052] The main function of the oxygen culture bottle 1 is to store oxygen culture liquid. The oxygen culture bottle 1 is further provided with a negative pressure connector 16 and a liquid inlet connector 17. The negative pressure connector 16 is connected with an external negative pressure tube, and the liquid inlet connector 17 is connected with a double-cannula on a patient's body. The double-cannula drainage liquid can be sucked into the oxygen culture bottle 1 through the oxygen culture bottle 1 by the negative pressure tube, so as to culture the drainage liquid.

[0053] The main function of the anaerobic culture bottle 3 is to store anaerobic culture liquid. The anaerobic culture bottle 3 is further provided with a negative pressure connector 16 and a liquid inlet connector 17. The negative pressure connector 16 is connected with an external negative pressure tube, and the liquid inlet connector 17 is connected with a double-cannula on a patient's body. The anaerobic culture bottle 3 is further provided with an air removal assembly 4 and an injection assembly 5. The air removal assembly 4 and the injection assembly 5 are connected, and the negative pressure connector 16 and the liquid inlet connector 17 are communicated with the air removal assembly 4 and the injection assembly 5.

[0054] The drainage liquid in the double cannula can be sucked into the injection assembly 5 through the negative pressure tube, and the air removal assembly 4 can remove the air carried when the drainage liquid is sucked, so that only the drainage liquid is left in the injection tube 501 in the injection assembly 5, without air, and the injection assembly 5 injects the drainage liquid after the air is removed into the anaerobic culture bottle 3 to culture the drainage liquid.

[0055] The main function of the culture bottle placing box 6 is to serve as a carrier for storing the aerobic culture bottle 1 and the anaerobic culture bottle 3, so as to facilitate the placement of the culture bottles, and also facilitate the medical staff to take and sample the drainage liquid.

[0056] As shown in FIGS. 1, 2 and 3, the aerobic culture bottle 1 comprises a bottle body 11, an aerobic bottle cap 12 and the elastic sealing assembly 2, the aerobic bottle cap 12 is detachably screwed on the bottle body 11, and the elastic sealing assembly 2 is arranged on the aerobic bottle cap 12 to controllably open and close the opening on the aerobic bottle cap 12.

[0057] The bottle body 11 is a hollow hard plastic bottle with a rectangular cross section, and PP plastic is preferred in the embodiment. In order to facilitate observation, the bottle body 11 is transparent, and in order to facilitate the installation of the aerobic bottle cap 12, a circular hole is formed on the upper end surface of the bottle body 11, and a thread is formed on the inner side wall of the circular hole. The through hole with the thread is named as a screw joint 15, and the aerobic bottle cap 12 is screwed on the bottle body 11 through the screw joint 15.

[0058] In order to facilitate the observation of the amount of the drainage liquid sucked into the bottle body 11 of the aerobic culture bottle 1, a scale 13 is printed on the outer side wall of the bottle body 11, and the amount of the drainage liquid sucked into the bottle body 11 can be conveniently checked through the scale 13.

[0059] In order to facilitate the user to hold the bottle body 11, a hand support 14 is fixed on one side of the bottle body 11, perpendicular to the side, and the hand support 14 is made of flexible plastic. When unfolded, the hand support 14 is half-ring-shaped, and when folded, the hand support 14 is sheet-shaped and adheres to the side wall of the bottle body 11. When sampling, the operator reversely buckles his hand into the hand support 14 (like wearing a ring), so that the hand support 14 can be fixed and facilitate the suction operation.

[0060] The aerobic bottle cap 12 is a plastic cap with a rectangular cross section, and an annular tube is protruded outwardly and perpendicularly on the end surface of the aerobic bottle cap 12. A thread is formed on the surface of the annular tube, and the aerobic bottle cap 12 can be screwed on the screw joint 15 of the bottle body 11 through the thread. This facilitates the pouring of the aerobic culture liquid into the bottle body 11 in the early stage, and also facilitates the sampling and detection of the culture liquid in the later stage of culture, and is convenient to use.

[0061] In order to ensure the sealing of the connection between the aerobic bottle cap 12 and the bottle body 11, a sealing gasket 18 is arranged on the end face of the aerobic bottle cap 12, and the sealing gasket 18 is arranged between the aerobic bottle cap 12 and the bottle body 11. When the bottle body 11 and the aerobic bottle cap 12 are screwed, the sealing gasket 18 can improve the sealing between the two.

[0062] In order to facilitate the drainage liquid to enter the aerobic culture bottle 1, a negative pressure connector 16 and a liquid inlet connector 17 are arranged on the upper end face of the aerobic bottle cap 12. The negative pressure connector 16 and the liquid inlet connector 17 are both circular tubular connectors, and the negative pressure connector 16 and the liquid inlet connector 17 are fixedly connected with the aerobic bottle cap 12 and communicate with the bottle body 11.

[0063] An external thread is formed on the outer side wall of the negative pressure connector 16 and the liquid inlet connector 17. Through the thread, an external negative pressure pipe connected with a negative pressure gauge can be screwed on the negative pressure connector 16 through a threaded connector. Meanwhile, the end part of a double cannula connected with a patient can be connected with the liquid inlet connector 17 through a threaded connector.

[0064] The negative pressure pipe and the negative pressure gauge are existing devices. The main function of the negative pressure gauge is to generate negative pressure. Through the negative pressure gauge, negative pressure suction force can be generated on the aerobic culture bottle 1, so that the drainage liquid is sucked into the aerobic culture bottle 1 through the double cannula and the liquid inlet connector 17, which is convenient to use.

[0065] In order to prevent external dust from falling into the aerobic culture bottle 1 and causing pollution to the culture liquid when not in use, a cover 19 is screwed on the negative pressure connector 16 and the liquid inlet connector 17. The cover 19 is connected with the negative pressure connector 16 or the liquid inlet connector 17 through a pulling rope. When in use, the cover 19 is unscrewed. After use, the cover 19 is screwed again to prevent external dust and sundries from falling into the aerobic culture bottle 1.

[0066] As shown in FIGS. 3, 4 and 5, in order to prevent the culture liquid in the bottle body 11 from leaking from the negative pressure connector 16 and the liquid inlet connector 17 due to shaking during transportation, and also to prevent dust from falling into the negative pressure connector 16 and the liquid inlet connector 17 when the cover 19 is opened and the negative pressure pipe or the double cannula is not connected, and in order to further limit the opening and closing of the negative pressure connector 16 and the liquid inlet connector 17, an elastic sealing assembly 2 is arranged in the negative pressure connector 16 and the liquid inlet connector 17. The elastic sealing assembly 2 controls the opening and closing of the negative pressure connector 16 and the liquid inlet connector 17.

[0067] The elastic sealing assembly 2 comprises a mounting rod 21, a sealing cone 22 and a first spring 24. The mounting rod 21 is a plastic rod with a rectangular cross section. The mounting rod 21 is fixed on the inner wall of the negative pressure connector 16 or the liquid inlet connector 17. A circular through hole penetrating through the mounting rod 21 is formed in the middle of the mounting rod 21 perpendicular to the mounting rod 21. The sealing cone 22 can be inserted into the circular through hole on the mounting rod 21.

[0068] The sealing cone 22 is a conical platform made of rubber. The larger end of the sealing cone 22 has a diameter slightly larger than the inner diameter of the negative pressure connector 16 and the liquid inlet connector 17, and the smaller end of the sealing cone 22 has a diameter smaller than the outer diameter of the negative pressure connector 16 and the liquid inlet connector 17. A cylindrical connecting rod 23 is fixed to the smaller end of the sealing cone 22. The connecting rod 23 can be inserted into the circular through hole of the mounting rod 21 and can move up and down along the circular through hole.

[0069] A circular spring mounting block 25 is fixed to the other end of the connecting rod 23. The diameter of the spring mounting block 25 is smaller than the inner diameter of the negative pressure connector 16 and the liquid inlet connector 17. The first spring 24 is sleeved on the connecting rod 23. One end of the first spring 24 abuts against the spring mounting block 25, and the other end abuts against the mounting rod 21. When the sealing cone 22 is pulled, the connecting rod 23 moves along the circular through hole of the mounting rod 21. At this time, the first spring 24 is compressed under force. After the sealing cone 22 is released, the sealing cone 22 is reset under the action of the first spring 24.

[0070] The elastic sealing assembly 2 is provided in the negative pressure connector 16 and the liquid inlet connector 17. The elastic sealing assembly 2 provided in the negative pressure connector 16 is located at the upper end of the negative pressure connector 16. The sealing cone 22 in the elastic sealing assembly 2 at this position is clamped on the outer end face of the negative pressure connector 16.

[0071] The elastic sealing assembly 2 provided in the liquid inlet connector 17 is located at the lower end of the liquid inlet connector 17. The sealing cone 22 in the elastic sealing assembly 2 at this position is clamped on the inner end face of the liquid inlet connector 17.

[0072] Regarding the sealing of the elastic sealing assembly 2 to the negative pressure connector 16 and the liquid inlet connector 17, when not in use, the sealing cone 22 is clamped on the port of the negative pressure connector 16 or the liquid inlet connector 17 under the action of the first spring 24. When negative pressure suction is performed, the sealing cone 22 in the elastic sealing assembly 2 at the negative pressure connector 16 is lifted upward under the action of negative pressure. At this time, the first spring 24 is compressed under force. When continuous negative pressure is applied, the sealing cone 22 in the elastic sealing assembly 2 at the inner end of the liquid inlet connector 17 moves downward under the action of negative pressure, and the liquid inlet connector 17 is opened. At this time, the drainage liquid in the double cannula can flow into the bottle body 11 through the liquid inlet connector 17. After use, the negative pressure is turned off. At this time, the sealing cone 22 in the negative pressure connector 16 and the liquid inlet connector 17 is reset under the action of the first spring 24, and the negative pressure connector 16 and the liquid inlet connector 17 are resealed.

[0073] Definition: In this embodiment, the bottle body 11 is taken as the reference. The direction in which the bottle body 11 points to the bottle cap is upward, the direction in which the bottle cap points to the bottle body 11 is downward, the direction from the outside to the bottle body 11 is internal, and the direction from the bottle body 11 to the outside is external.

[0074] As shown in Fig. 1, 6, 7, 8, 9, 10 and 12, the anaerobic culture bottle 3 comprises a bottle body 11 and an anaerobic bottle cap 31 which is detachably screwed together with the bottle body 11.

[0075] The bottle body 11 of the aerobic culture bottle 1 is completely identical with the bottle body 11 of the anaerobic culture bottle 3, and the anaerobic bottle cap 31 is basically identical with the aerobic bottle cap 12, both of which comprise a negative pressure connector 16 and a liquid inlet connector 17. The difference between the anaerobic bottle cap 31 and the aerobic bottle cap 12 is that a functional hole 32 for assisting the installation of the injection assembly 5 is formed on the anaerobic bottle cap 31, and the air removal assembly 4 for removing air in the drainage liquid is also provided on the anaerobic bottle cap 31.

[0076] The main function of the air removal assembly 4 is to remove the air in the drainage liquid sucked into the injection tube 501 of the injection assembly 5, so that the injection tube 501 is finally filled with the drainage liquid without air.

[0077] The main function of the injection assembly 5 is to inject the drainage liquid without air into the anaerobic culture bottle 3, so as to facilitate the anaerobic culture of the drainage liquid.

[0078] The air removal assembly 4 comprises an overflow compartment 41, a functional compartment 42 and an exhaust pipe 43. The functional compartment 42 is connected with the overflow compartment 41, and the exhaust pipe 43 is connected at one end with the functional compartment 42 and at the other end with the negative pressure connector 16 on the anaerobic bottle cap 31.

[0079] The overflow compartment 41 is a circular tube made of plastic, and the upper and lower ends of the overflow compartment 41 are closed. The main function of the overflow compartment 41 is to store the drainage liquid overflowing from the injection tube 501.

[0080] In order to facilitate the connection with the negative pressure connector 16 and thus realize negative pressure suction, an arc-shaped cavity is protruded outward on the upper surface of the functional compartment 42, which is named as the functional compartment 42. The lower end of the functional compartment 42 is in communication with the overflow compartment 41, and a circular connecting pipe is provided on the upper end surface of the functional compartment 42, which is named as the exhaust pipe 43. The exhaust pipe 43 covers the lower end of the negative pressure connector 16 on the anaerobic bottle cap 31, and a sealing treatment is performed at the covering position. Thus, when the negative pressure pipe is connected to the negative pressure connector 16, the overflow compartment 41 will also be in a negative pressure state.

[0081] In order to facilitate the installation of the injection assembly 5, a circular through hole is formed on the upper end surface of the overflow compartment 41. The injection tube 501 in the injection assembly 5 is inserted into the circular through hole, and a sealing treatment is performed at the connection position between the injection tube 501 and the overflow compartment 41.

[0082] The injection assembly 5 comprises an injection tube 501, an injection push rod 502 and an exhaust port sealing rod 503. The injection tube 501 is fixed at one end of the anaerobic bottle cap 31 and covers the functional hole 32, and is inserted into the overflow bin 41 at the other end. In order to ensure the sealing, prevent liquid and gas leakage at the contact, the injection tube 501 is sealed at the connection. The exhaust port sealing rod 503 is arranged parallel to the injection push rod 502, penetrates the push plate on the injection push rod 502, and is parallel to the injection push rod 502. The injection push rod 502 and the exhaust port sealing rod 503 are inserted into the injection tube 501 through the anaerobic bottle cap 31.

[0083] The injection tube 501 is a cylindrical tube, and the shape is similar to that of a syringe. The upper end of the injection tube 501 is open, the injection push rod 502 and the exhaust port sealing rod 503 are inserted into the injection tube 501 through the opening, and the lower end of the injection tube 501 protrudes outwardly and has an injection port 505. The injection port 505 penetrates the bottom surface of the overflow bin 41 and communicates with the bottle body 11 of the anaerobic culture bottle 3. The injection port 505 can inject the drainage liquid without air into the bottle body 11 of the anaerobic culture bottle 3.

[0084] The injection push rod 502 is similar to the push rod of a common syringe, and comprises an injection piston 507 and a push plate. The injection piston 507 is inserted into the injection tube 501 through the functional hole 32. The injection piston 507 can inject the drainage liquid in the injection tube 501 into the bottle body 11 of the anaerobic culture bottle 3.

[0085] In order to form a negative pressure suction passage, a circular through hole penetrating the injection piston 507 is formed on the surface of the injection piston 507, which is named as a second exhaust port 508. A circular hole penetrating the single side wall of the injection tube 501 is formed on the side wall of the injection tube 501 close to the upper end of the injection tube 501 and the functional bin 42, which is named as a first exhaust port 506. A circular through hole is also formed on the corresponding position of the functional bin 42. The overflow bin 41 and the injection tube 501 can be communicated through the circular through hole, the first exhaust port 506 and the second exhaust port 508.

[0086] A circular hole penetrating the single side wall of the injection tube 501 is formed on the side wall of the injection tube 501 close to the upper end, which is named as a liquid inlet 504. The height of the liquid inlet 504 is slightly lower than that of the first exhaust port 506. An “L”-shaped pipeline is connected to the liquid inlet 504, which is named as a liquid inlet pipeline 512. One end of the liquid inlet pipeline 512 is connected to the liquid inlet 504, and the other end covers the lower end of the liquid inlet joint 17 on the lower end surface of the anaerobic bottle cap 31. In order to ensure that the sealing cone 22 in the elastic sealing assembly 2 in the liquid inlet joint 17 can be smoothly opened, the inner diameter of the liquid inlet pipeline 512 is greater than the outer diameter of the sealing cone 22.

[0087] A passage is formed by the liquid inlet pipe 512, the liquid inlet 504, the second air outlet 508, the first air outlet 506 and the air outlet pipe 43. When negative pressure suction is applied, the drainage liquid in the double cannula can be sucked into the injection pipe 501 through the liquid inlet connector 17 and the liquid inlet pipe 512, and the air sucked in together with the drainage liquid can be discharged through the first air outlet 506 and the second air outlet 508.

[0088] When the injection pipe 501 is filled with the drainage liquid without air, in order to prevent the drainage liquid from entering the overflow chamber 41 through the first air outlet 506 and the second air outlet 508 during injection, the second air outlet 508 can be closed by the air outlet sealing rod 503.

[0089] The air outlet sealing rod 503 includes a sealing head 509 made of rubber, and the sealing head 509 is slightly larger than the size of the second air outlet 508. The sealing head 509 is fixed to one end of the air outlet sealing rod 503, and by pressing the air outlet sealing rod 503, the sealing head 509 can be inserted into the second air outlet 508 to close the second air outlet 508.

[0090] In order to make the closure of the second air outlet 508 controllable, a limiting ring 511 is fixed on the rod of the air outlet sealing rod 503, and a spring is sleeved on the air outlet sealing rod 503, which is named as the second spring 510. One end of the second spring 510 abuts against the anaerobic bottle cap 31, and the other end abuts against the limiting ring 511. When the air outlet sealing rod 503 does not close the second air outlet 508, the air outlet sealing rod 503 is suspended under the action of the second spring 510, and the opening of the second air outlet 508 is the initial state. In the initial state, the injection piston 507 is located between the liquid inlet 504 and the first air outlet 506.

[0091] In order to prevent the gas in the bottle body 11 of the anaerobic culture bottle 3 from being sucked away during negative pressure suction, a sealing piece 513 is hinged to the overflow chamber 41 through a hinge table. One end of the sealing piece 513 is hinged to the hinge table, and the other end covers the injection port 505 to close the injection port 505. In order to make the sealing piece 513 close the injection port 505 in the normal state, a torsional spring is sleeved on the hinge rod of the sealing piece 513. The torsional spring applies force to the sealing piece 513, so that the sealing piece 513 can close the injection port 505 in the normal state. When the injection pipe 501 is injected, the liquid in the injection pipe 501 presses the sealing piece 513 to open the sealing piece 513 along the hinge axis. When the injection is stopped, the sealing piece 513 closes the injection port 505 under the action of the torsional spring.

[0092] As shown in FIGS. 6, 11 and 12, in order to make the injection of the injection push rod 502 controllable, a push rod limiting assembly 7 is arranged on the anaerobic bottle cap 31. The push rod limiting assembly 7 includes a limiting rod 71 and a limiting clamp 73.

[0093] The limiting rod 71 is a rectangular rod made of plastic, one end of the limiting rod 71 is fixed on the upper surface of the anaerobic bottle cap 31, the other end passes through the push plate on the injection push rod 502, the injection push rod 502 can move up and down along the limiting rod 71.

[0094] A plurality of rectangular grooves are vertically provided on one side of the limiting rod 71, which are named as limiting grooves 72, and the limiting grooves 72 are spaced apart on the limiting rod 71.

[0095] The limiting card 73 is a card with a "Z" shaped cross section, the limiting card 73 is hinged on the push plate in the injection push rod 502 through a hinge table, one end of the limiting card 73 can be clamped in the limiting groove 72 to limit the injection push rod 502, in order to make the limiting of the injection push rod 502 by the limiting card 73 controllable, a torsional spring is sleeved on the hinge shaft of the limiting card 73, under the action of the torsional spring, one end of the limiting card 73 is always clamped in the limiting groove 72, only by manually pressing the other end of the limiting card 73 can the clamping relationship between the limiting card 73 and the limiting groove 72 be released, thereby realizing the controllable control of the injection of the injection push rod 502.

[0096] Regarding the process of negative pressure suction of drainage liquid into the anaerobic culture bottle 3: in use, first, the cover 19 on the anaerobic bottle cap 31 is unscrewed, then the negative pressure pipe is screwed to the negative pressure connector 16, the double cannula is connected to the liquid inlet connector 17, then the negative pressure gauge on the negative pressure pipe is opened, at this time, under the action of negative pressure, the drainage liquid in the double cannula flows into the injection pipe 501 through the liquid inlet connector 17 and the liquid inlet pipe 512, then the negative pressure suction is continued until the injection pipe 501 is filled with drainage liquid, the overflowed drainage liquid flows into the overflow warehouse 41 through the first exhaust port 506 and the second exhaust port 508, at this time, the negative pressure suction can be stopped, then the exhaust port sealing rod 503 is pressed down, the sealing head 509 on the exhaust port sealing rod 503 is used to close the second exhaust port 508, then the limiting card 73 on the push rod limiting assembly 7 is pressed to release the limitation of the position of the injection push rod 502, then the injection push rod 502 is pushed, the drainage liquid in the injection pipe 501 extrudes the sealing sheet 513 at the injection port 505, the sealing sheet 513 is opened, the drainage liquid in the injection pipe 501 can be injected into the bottle body 11 of the anaerobic culture bottle 3, after the appropriate amount is injected, the injection push rod 502 is stopped, the limiting card 73 is released, at this time, the limiting card 73 is clamped in the limiting groove 72 in the limiting rod 71 to limit the position of the injection push rod 502;

[0097] When the double cannula is not enough to fill the injection tube 501, the second exhaust port 508 is not closed before the injection push rod 502 is pushed forward, and the cover 19 on the negative pressure connector 16 is opened. At this time, the gas in the injection tube 501 will be discharged from the negative pressure connector 16 first (the elastic force of the torsional spring in the sealing piece 513 needs to be greater than the elastic force of the first spring 24 in the negative pressure connector 16), until part of the drainage liquid flows into the overflow chamber 41, and then the second exhaust port 508 is closed for injection, ensuring that the drainage liquid injected into the bottle body 11 of the anaerobic culture bottle 3 does not contain air, ensuring the accuracy of anaerobic culture.

[0098] In addition, when the injection push rod 502 injects into the bottle body 11, in order to facilitate the smooth entry of the drainage liquid in the injection tube 501 into the bottle body 11, a one-way valve can be installed on the bottle body 11 of the anaerobic culture bottle 3. During injection, part of the gas in the bottle body 11 is discharged through the one-way valve (this gas is pre-charged inert gas, which is prior art), at this time the drainage liquid in the injection tube 501 can be smoothly injected into the bottle body 11.

[0099] In order to facilitate the observation of the amount of drainage liquid, the injection tube 501 and the overflow chamber 41 are preferably made of transparent material.

[0100] As shown in Figure 13, the culture bottle placing box 6 is a box with a rectangular cross section, the upper end of the culture bottle placing box 6 is open, and the aerobic culture bottle 1 and the anaerobic culture bottle 3 can be placed in the culture bottle placing box 6.

[0101] A rectangular partition plate 61 is vertically protruding from the bottom surface of the culture bottle placing box 6 in the middle of the culture bottle placing box 6, which divides the culture bottle placing box 6 into two culture bottle placing cavities 63 of the same size through the partition plate 61, and the aerobic culture bottle 1 and the anaerobic culture bottle 3 are placed in the two culture bottle placing cavities 63 respectively.

[0102] In order to facilitate the observation of the aerobic culture bottle 1 and the anaerobic culture bottle 3 placed in the culture bottle placing cavities 63, a rectangular observation window 62 is formed on each of the two side surfaces of the culture bottle placing box 6, through which the aerobic culture bottle 1 and the anaerobic culture bottle 3 placed in the culture bottle placing cavities 63 can be observed.

[0103] The method for using the double cannula continuous flushing negative pressure suction liquid culture medium set includes the following steps:

[0104] Step one: preparation: prepare the culture medium set to be used, and confirm and fill in the patient information, paste the patient information on the side wall of the aerobic culture bottle 1 and the anaerobic culture bottle 3, and pay attention not to cover the scale 13, and then prepare the negative pressure tube and negative pressure gauge for connection before use.

[0105] Step two: aerobic culture bottle 1 drainage liquid collection:

[0106] S21, take out the aerobic culture bottle 1 from the culture bottle placing box 6, put the hand into the hand holder 14 in reverse, then unscrew the negative pressure connector 16 on the aerobic bottle cap 12 and the sealing cover 19 on the liquid inlet connector 17.

[0107] S22, connect the negative pressure pipe with the negative pressure gauge to the negative pressure connector 16, and connect the double cannula connected with the patient to the liquid inlet connector 17.

[0108] S23, open the negative pressure gauge, the negative pressure gauge provides negative pressure suction, the drainage fluid in the double cannula is sucked into the bottle body 11 of the aerobic culture bottle 1 through the liquid inlet connector 17, and the amount of the sucked drainage fluid is observed through the scale on the bottle body 11, when the amount is enough, close the negative pressure gauge.

[0109] S24, remove the negative pressure pipe on the negative pressure connector 16 and the double cannula on the liquid inlet connector 17, then reattach the sealing cover 19 to the negative pressure connector 16 and the liquid inlet connector 17.

[0110] S25, the aerobic culture bottle 1 drainage fluid collection is completed, the aerobic culture bottle 1 is placed in one of the culture bottle placing cavities 63 in the culture bottle placing box 6.

[0111] Step three: anaerobic culture bottle 3 drainage fluid collection:

[0112] S31, take out the anaerobic culture bottle 3 from the culture bottle placing box 6, put the hand into the hand holder 14 in reverse, then unscrew the negative pressure connector 16 on the anaerobic bottle cap 31 and the sealing cover 19 on the liquid inlet connector 17.

[0113] S32, install the negative pressure pipe connected with the negative pressure gauge to the negative pressure connector 16, and connect the double cannula connected with the patient to the liquid inlet connector 17.

[0114] S33, open the negative pressure gauge, the negative pressure gauge provides negative pressure suction, the drainage fluid in the double cannula is sucked into the injection pipe 501 in the injection assembly 5 through the liquid inlet connector 17, the drainage fluid accumulates at the bottom of the injection pipe 501, the air in the injection pipe 501 is discharged through the exhaust port as the drainage fluid is sucked into the injection pipe 501, until the injection pipe 501 is filled with drainage fluid or the drainage fluid in the injection pipe 501 reaches a reasonable value, close the negative pressure gauge and stop the negative pressure suction.

[0115] S34, inject the drainage fluid in the injection pipe 501 into the bottle body 11 of the anaerobic culture bottle 3, when the injection pipe 501 is filled with drainage fluid without air, go to step S35, when there is air in the injection pipe 501, go to step S36.

[0116] S35, press the limit card 73, unlock the injection push rod 502, push the injection push rod 502 in the injection assembly 5 to inject the drainage fluid into the bottle body 11 of the anaerobic culture bottle 3, and observe the injection amount of the drainage fluid through the scale 13.

[0117] S36, press the limit card 73, and unlock the injection push rod 502, push the injection push rod 502 in the injection assembly 5 to use the air cleaning assembly 4 to discharge the air in the injection tube 501, and then push the injection push rod 502 to inject the drainage liquid into the bottle body 11 of the anaerobic culture bottle 3, and observe the injection amount of the drainage liquid through the scale 13.

[0118] S37, remove the negative pressure pipe on the negative pressure connector 16 and the double cannula on the liquid inlet connector 17, and then cover the cover 19 on the negative pressure connector 16 and the liquid inlet connector 17 again.

[0119] S38, the anaerobic culture bottle 3 drainage liquid collection is completed, and the anaerobic culture bottle 3 is placed in another culture bottle placing cavity 63 in the culture bottle placing box 6.

[0120] Step four: after the aerobic culture bottle 1 and the anaerobic culture bottle 3 drainage liquid collection is completed, fill in the collection information on the culture bottle placing box 6, and send the collected aerobic culture bottle 1 and the anaerobic culture bottle 3 to culture.

[0121] The above examples only illustrate the technical idea of the present application, and cannot limit the protection scope of the present application. Any modification made according to the technical idea of the present application on the basis of the technical scheme falls within the protection scope of the present application.

Claims

1. A method of using a double-cannula continuous flush negative pressure suction liquid media set, comprising: The method comprises the following steps: Step one: preparation: prepare the culture medium set to be used, and confirm and fill in the patient information, paste the patient information on the side wall of the aerobic culture bottle (1) and the anaerobic culture bottle (3), and prepare the negative pressure tube and the negative pressure gauge for negative pressure suction; Step two: collection of the drainage liquid in the aerobic culture bottle (1): S21, take out the aerobic culture bottle (1) from the culture bottle placing box (6), and then remove the negative pressure connector (16) on the aerobic bottle cap (12) and the sealing cover (19) on the liquid inlet connector (17); S22, install the negative pressure tube connected with the negative pressure gauge on the negative pressure connector (16), and connect the double cannula connected with the patient with the liquid inlet connector (17); S23, open the negative pressure gauge, the negative pressure gauge provides negative pressure suction, the drainage liquid in the double cannula is sucked into the bottle body (11) in the aerobic culture bottle (1) through the liquid inlet connector (17), and the amount of the sucked drainage liquid is observed through the scale on the bottle body (11), when the amount is enough, the negative pressure gauge is closed; S24, remove the negative pressure tube on the negative pressure connector (16) and the double cannula on the liquid inlet connector (17), and then cover the sealing cover (19) on the negative pressure connector (16) and the liquid inlet connector (17) again; S25, the collection of the drainage liquid in the aerobic culture bottle (1) is completed, and the aerobic culture bottle (1) is placed in one of the culture bottle placing cavities (63) in the culture bottle placing box (6); Step three: collection of the drainage liquid in the anaerobic culture bottle (3): S31, take out the anaerobic culture bottle (3) from the culture bottle placing box (6), and then remove the negative pressure connector (16) on the anaerobic bottle cap (31) and the sealing cover (19) on the liquid inlet connector (17); S32, install the negative pressure tube connected with the negative pressure gauge on the negative pressure connector (16), and connect the double cannula connected with the patient with the liquid inlet connector (17); S33, open the negative pressure gauge, the negative pressure gauge provides negative pressure suction, the drainage liquid in the double cannula is sucked into the injection tube (501) in the injection assembly (5) through the liquid inlet connector (17), the drainage liquid accumulates at the bottom of the injection tube (501), the air in the injection tube (501) is discharged through the air outlet as the drainage liquid is sucked into the injection tube (501), until the injection tube (501) is filled with the drainage liquid or the drainage liquid in the injection tube (501) reaches a proper value, the negative pressure gauge is closed, and the negative pressure suction is stopped; S34, inject the drainage liquid in the injection tube (501) into the bottle body (11) of the anaerobic culture bottle (3), when the injection tube (501) is filled with the drainage liquid without air, go to step S35, and when the injection tube (501) has air, go to step S36; S35, push the injection push rod (502) in the injection assembly (5) to inject the drainage liquid into the bottle body (11) of the anaerobic culture bottle (3), and observe the injection amount of the drainage liquid through the scale (13); S36, push the injection push rod (502) in the injection assembly (5) to use the air removal assembly (4) to discharge the air in the injection tube (501), and then push the injection push rod (502) to inject the drainage liquid into the bottle body (11) of the anaerobic culture bottle (3), and observe the injection amount of the drainage liquid through the scale (13); S37, remove the negative pressure pipe on the negative pressure connector (16) and the double sleeve pipe on the liquid inlet connector (17), and then cover the cover (19) on the negative pressure connector (16) and the liquid inlet connector (17) again; S38, after the drainage liquid collection of the anaerobic culture bottle (3) is completed, the anaerobic culture bottle (3) is placed into another culture bottle placing cavity (63) in the culture bottle placing box (6); Step four: after the drainage liquid collection of the aerobic culture bottle (1) and the anaerobic culture bottle (3) is completed, fill in the collection information on the culture bottle placing box (6), and send the collected aerobic culture bottle (1) and anaerobic culture bottle (3) to culture.

2. The method of using a dual-cannula continuous irrigation negative- pressure wound therapy fluid culture media set of claim 1, wherein: In S33 of step three, the injection tube (501) is communicated with the air removal assembly (4), and the overflowed drainage liquid in the injection tube (501) is stored in the overflow storage (41) in the air removal assembly (4).

3. The method of using a dual-cannula continuous irrigation negative- pressure wound therapy fluid culture media set of claim 1, wherein: In S35 and S36 of step three, the injection push rod (502) is connected with the push rod limiting assembly (7), and the injection push rod (502) is limited by the push rod limiting assembly (7).

4. The method of using a dual-cannula continuous irrigation negative- pressure wound therapy fluid culture media kit of claim 1, wherein: The culture medium set includes the aerobic culture bottle (1), the anaerobic culture bottle (3) and the culture bottle placing box (6), the aerobic culture bottle (1) and the anaerobic culture bottle (3) include the bottle body (11) and the bottle cap, the bottle cap is screwed on the bottle body (11) to close the bottle body (11), and the aerobic culture bottle (1) storing aerobic culture liquid and the anaerobic culture bottle (3) storing anaerobic culture liquid can be placed in the culture bottle placing box (6) in a detachable manner.

5. The method of using a dual-cannula continuous irrigation negative- pressure wound therapy fluid culture media set of claim 4, wherein: The anaerobic culture bottle (3) includes the air removal assembly (4) and the injection assembly (5), the injection assembly (5) is connected with the liquid inlet connector (17) on the anaerobic culture bottle (3), the air removal assembly (4) is connected with the negative pressure connector (16) on the anaerobic culture bottle (3), the air removal assembly (4) is communicated with the injection assembly (5), the air removal assembly (4) discharges the air sucked into the injection tube (501) in the injection assembly (5), and the injection assembly (5) injects the suction liquid without air into the bottle body (11) storing the anaerobic bacteria culture liquid.

6. The dual-cannula continuous irrigation negative- pressure wound therapy kit of claim 4, wherein: The bottle body (11) is made of transparent material, and a scale (13) for observing the amount of liquid in the bottle body (11) is arranged on the side wall of the bottle body (11).

7. The dual-cannula continuous irrigation negative- pressure wound therapy kit of claim 4, wherein: The culture bottle placing box (6) includes a partition plate (61) and an observation window (62), the partition plate (61) is arranged in the culture bottle placing box (6) to divide the culture bottle placing box (6) into two culture bottle placing cavities (63), and the observation window (62) is arranged on the two sides of the culture bottle placing box (6), and the amount of liquid in the bottle body (11) is observed through the observation window (62).

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