Imaging device

Abstract

An imaging device (1), comprising: an optical assembly (10), a focus-maintaining element (20), a focusing assembly (30), and a mounting frame (40). The focus-maintaining element (20) is movably arranged on the mounting frame (40); the focusing assembly (30) comprises a passive focusing element (31) and an active focusing element (32); and the optical assembly (10) is arranged on the active focusing element (32). When the imaging device (10) is in a passive focusing mode, the focus-maintaining element (20) always abuts against an area to be observed of a sample plate, the passive focusing element (31) always abuts against the focus-maintaining element (20) and moves synchronously with the focus-maintaining element (20), and the optical assembly (10) and the passive focusing element (31) are relatively fixed, so that the distance from the optical assembly (10) to the area to be observed of the sample plate can be relatively constant.

Description

Imaging device

[0001] The present application claims priority to the Chinese patent application No. 202411042275.1, filed on July 31, 2024, and entitled “Imaging device”, the content of which is incorporated herein by reference in its entirety. TECHNICAL FIELD

[0002] The present application relates to the technical field of mechanical equipment, in particular to an imaging device. BACKGROUND

[0003] The imaging device is a commonly used instrument for observing a sample plate as a to-be-observed object. However, the sample plate is prone to product manufacturing errors in the production process, or the sample plate is prone to deformation due to accidents, which will cause the to-be-observed area of the sample plate to be at least partially unable to be located in the optimal imaging area when being observed, thereby affecting the imaging quality. TECHNICAL PROBLEM

[0004] To solve the above problems, the present application provides an imaging device, which comprises an optical assembly, a focusing piece, a focusing assembly and a mounting rack, the focusing piece is movably arranged on the mounting rack, the focusing assembly comprises a follow-up focusing piece and a main focusing piece, the optical assembly is arranged on the main focusing piece, the imaging device has a follow-up focusing mode, in the follow-up focusing mode, the focusing piece always abuts against the to-be-observed area of the sample plate, the follow-up focusing piece always abuts against the focusing piece and moves synchronously with the focusing piece, the optical assembly 10 is relatively fixed with the follow-up focusing piece, so that the distance from the optical assembly to the to-be-observed area of the sample plate is relatively constant, in other modes, the main focusing piece can drive the optical assembly to move independently relative to the focusing piece and / or the follow-up focusing piece, so as to adjust the distance from the optical assembly to the sample plate or the relative position between the optical assembly and the focusing piece. TECHNICAL SOLUTION

[0005] If the optical assembly is located at a fixed position to image each to-be-observed area of the sample plate, when the surface of the sample plate is not flat enough or the sample plate is placed obliquely, the distances from each to-be-observed area to the optical assembly can not be the same, part of the to-be-observed areas are located at appropriate imaging positions, and part of the to-be-observed areas are located at inappropriate imaging positions. In the embodiments of the present application, the focusing piece can always contact the to-be-observed area to be observed during imaging of each to-be-observed area, so that the focusing piece can move synchronously according to the position of the to-be-observed area. In other words, the focusing piece can adaptively adjust the position of the optical assembly according to the position of the to-be-observed area, so that the distance from the optical assembly to the to-be-observed area being observed is always relatively constant when observing different to-be-observed areas, which is beneficial to capturing clear images of each to-be-observed area, thereby improving the accuracy of cell counting.

[0006] In addition, if the relative position of the optical assembly and the focusing member cannot be adjusted, the focal length manufacturing error of the optical assembly or the shape error of the focusing member can cause the focusing member to always fail to obtain a clear image when the focusing member contacts the observation area. Therefore, the present application adjusts the relative position of the optical assembly and the focusing member through the focusing assembly to improve the above phenomenon. BRIEF DESCRIPTION OF DRAWINGS

[0007] FIG. 1 is a schematic diagram of the imaging principle of an imaging device according to one or more embodiments;

[0008] FIG. 2 is a schematic diagram of the structure of an imaging device according to one or more embodiments;

[0009] FIG. 3 is a schematic diagram of a focusing assembly according to one or more embodiments;

[0010] FIG. 4 is a schematic diagram of the structure of a focusing member according to one or more embodiments;

[0011] FIG. 5 is a schematic diagram of the working principle of a focusing member when deflection occurs according to one or more embodiments;

[0012] FIG. 6 is a schematic diagram of the structure of a sample plate carried by a bearing platform according to one or more embodiments;

[0013] FIG. 7 is a schematic diagram of a transition protrusion abutting against a first region of a focusing member to space the contact protrusion of the focusing member and the sample plate apart according to one or more embodiments;

[0014] FIG. 8 is a schematic diagram of a contact protrusion abutting against a sample plate according to one or more embodiments.

[0015] REFERENCE SIGNS:

[0016] imaging device 1,

[0017] optical assembly 10, objective lens 11, lens barrel 12, imaging unit 13,

[0018] focusing member 20, main body portion 200, top wall 2001, side wall 2002, contact protrusion 201, first protrusion 201a, second protrusion 201b, observation window 202, first region 203, second region 204, transition region 205,

[0019] focusing assembly 30, follow-up adjusting member 31, mounting block 311, abutting block 312, active focusing member 32, driving motor 321, transmission screw 322, screw nut 323, elastic member 33,

[0020] mounting bracket 40, first adapter block 41, accommodating groove 411,

[0021] Bearing platform 50, placement area 501, fixing groove 502, opening 503, transition bump 51. Embodiments of the present application

[0022] The application will be further described below in conjunction with the accompanying drawings and some embodiments. The following embodiments are mainly used to exemplarily illustrate the technical solutions of the application, and thus cannot be used to limit the protection scope of the application.

[0023] Unless otherwise defined, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein mainly serves the purpose of describing specific embodiments of the application, and is not intended to limit the application; the terms "comprise", "have", "contain" and other synonymous terms with the same or similar meanings in the specification and claims of the application and the above description of the drawings are intended to cover non-exclusive inclusion.

[0024] In the description of the embodiments of the application, the technical terms "first", "second", etc. mainly serve the purpose of facilitating the distinction between different objects, and cannot be understood as explicitly or implicitly indicating relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the indicated technical features. In the description of the embodiments of the application, the meaning of "a plurality of" is two or more, unless otherwise explicitly and specifically limited.

[0025] In this document, a specific feature, structure or property described in any one embodiment can be included in at least one embodiment of the application or a combination of at least two embodiments. Those skilled in the art can understand that the embodiments described herein can be combined with other embodiments herein or other embodiments outside this document.

[0026] In the description of the embodiments of the application, the technical terms used to indicate the position or positional relationship, such as "center", "lengthwise", "transverse", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., mainly serve the purpose of facilitating the description of the embodiments of the application and simplifying the description, and are not considered as indicating that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be understood as limiting the application.

[0027] In various embodiments of the present application, unless specifically defined and limited otherwise, the terms "set", "install", "assemble", "connect", "connect", "fix" and other terms should be broadly interpreted. For example, it can include fixed connection, detachable connection or integral molding; it can include at least one of mechanical connection and electrical connection; it can include direct connection or indirect connection through intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of the present application according to the specific circumstances.

[0028] The present inventors have found that in the process of cell counting using a sample plate, due to factors such as the sample plate may be tilted during placement, the sample plate may be warped, the sample plate may have large manufacturing errors, etc., the various to-be-observed areas of the sample plate may not be simultaneously located at a suitable imaging position (e.g., the distance to the objective / optical assembly is too large or too small), which may result in that the captured images of some to-be-observed areas are not clear enough, and thus affect the accuracy of cell counting.

[0029] For example, in FIG. 1, due to the surface of the sample plate is not flat enough (may be due to manufacturing error, or may be due to warping), the distances from the areas A and B on the surface of the sample plate to the optical assembly 10 are different. If the position of the optical assembly 10 is relatively fixed when observing the areas A and B, when the optical assembly 10 can obtain a clear image of the area A, the image of the area B obtained is probably not clear enough. In this case, the user may need to manually focus to obtain a clear image. However, manually focusing by the user also brings many problems, such as low imaging efficiency, poor user experience, etc.

[0030] To solve the above problems, the present application adds a focusing piece 20 between the optical assembly 10 and the sample plate. When observing different to-be-observed areas of the sample plate, the optical assembly 10 and the focusing piece 20 are connected, and the focusing piece 20 can always contact the to-be-observed area being observed, that is, the focusing piece 20 and the optical assembly 10 can always adaptively move following the position of the to-be-observed area being observed, so that the distance from the optical assembly 10 to the to-be-observed area being observed is relatively constant. That is, for example, the area B is farther away from the optical assembly 10 than the area A, and during the process of switching from observing the area A to observing the area B, the optical assembly 10 can adaptively move towards the sample plate, so that when observing the area B, the distance between the optical assembly 10 and the area B is within a suitable range.

[0031] Referring to FIG. 1, FIG. 2 and FIG. 3, according to one or more embodiments of the present application, the imaging device 1 can include an optical assembly 10, a focusing piece 20, a focusing assembly 30 and a mounting frame 40. The focusing piece 20 is movably arranged on the mounting frame 40. The focusing assembly 30 includes a following focusing piece 31 and an active focusing piece 32. The optical assembly 10 is arranged on the active focusing piece 32. The optical assembly 10 can include an objective lens 11, a lens barrel 12 and an imaging unit 13 close to the focusing piece 20. The imaging unit 13 can be a CCD camera or a CMOS camera.

[0032] The imaging device 1 has a following focusing mode. In the following focusing mode, the focusing piece 20 always abuts against the to-be-observed region of the sample plate, the following focusing piece 31 always abuts against the focusing piece 20 and moves synchronously with the focusing piece 20, and the optical assembly 10 is relatively fixed with the following focusing piece 31, so that the distance between the optical assembly 10 and the to-be-observed region of the sample plate is relatively constant.

[0033] In the embodiments of the present application, during imaging of each to-be-observed region, the focusing piece 20 can always contact the to-be-observed region to be observed, so that the focusing piece 20 can move synchronously according to the position of the to-be-observed region. In other words, the focusing piece 20 can adaptively adjust the position of the optical assembly 10 according to the position of the to-be-observed region, so that the distance between the optical assembly 10 and the to-be-observed region being observed is always relatively constant when the optical assembly 10 observes different to-be-observed regions, which is beneficial to capturing clear images of each to-be-observed region, thereby improving the accuracy of cell counting.

[0034] In addition, considering that if the relative position of the optical assembly 10 and the focusing piece 20 is not adjustable, the focal length manufacturing error of the optical assembly 10 or the shape error of the focusing piece 20 can cause the optical assembly 10 to always fail to obtain a clear image when the focusing piece 20 contacts the to-be-observed region. Therefore, the present application further adjusts the relative position of the optical assembly 10 and the focusing piece 20 through the active focusing piece 32 to improve the above phenomenon. After the optical assembly 10 is driven to move to a suitable position, the driving of the optical assembly 10 can be stopped, so that the position of the optical assembly 10 and the focusing piece 20 is maintained relatively fixed, and in this position, the optical assembly 10 can clearly image the region abutted by the focusing piece 20.

[0035] In other modes, the active focusing member 32 can actively drive the optical assembly 10 to move independently relative to the fixed focusing member 20 and / or the passive focusing member 31, so as to adjust the distance of the optical assembly 10 to the sample plate or the relative position between the optical assembly 10 and the fixed focusing member 20. Specifically, in a debugging mode, if it is found that the optical assembly 10 cannot always obtain a clear image, the position of the optical assembly 10 can be actively adjusted by the active focusing member 32 until a clear image can be obtained, while keeping the passive focusing member 31 abutting against the fixed focusing member 20 and the fixed focusing member 20 abutting against the sample plate. Alternatively, when the cell stratification of the sample plate is obvious, the focusing assembly 30 can also be switched to the active focusing mode, in which case the optical assembly 10 is driven to adjust the distance of the optical assembly 10 to the sample plate for imaging.

[0036] Referring to FIGS. 2, 3 and 4, according to one or more embodiments of the present application, the fixed focusing member 20 has a contact bump 201 protruding towards the side away from the optical assembly 10 for abutting against the sample plate. In this case, the shape of the contact bump 201 is beneficial to reduce the contact area between the sample plate and the fixed focusing member 20, so that the fixed focusing member 20 can also respond sensitively when the surface of the sample plate is slightly deformed.

[0037] Ideally, the contact area between the contact bump 201 and the sample plate can be approximately a line or 1 or 2 points. For example, the protruding surface of the contact bump 201 can be a cylindrical surface, and the contact area between the cylindrical surface and the surface of the sample plate can be approximately a line. For another example, the protruding surface of the contact bump 201 can be a spherical surface, and the contact area between the spherical surface and the surface of the sample plate can be approximately a point.

[0038] Referring to FIG. 4, according to one or more embodiments of the present application, the fixed focusing member 20 can include a main body portion 200 and a contact bump 201 provided on the main body portion 200. The main body portion 200 is provided with an observation window 202. The optical assembly 10 can observe the sample plate through the observation window 202. The contact bump 201 can include a first bump 201a and a second bump 201b provided on both sides of the observation window 202 and protruding towards the side away from the optical assembly 10. The first bump 201a and the second bump 201b can be mirror-symmetrically arranged. In this case, the view of the optical assembly 10 can be avoided from being blocked by the contact bump 201 to a certain extent.

[0039] Referring to FIGS. 2 and 3, according to one or more embodiments of the present application, the mounting frame 40 can include a first adapter block 41. The focusing member 20 can be slidably disposed in the first adapter block 41. The sliding direction of the focusing member 20 is parallel to the spacing direction between the sample plate and the optical assembly 10. The focusing member 20 can slide according to the position of the region to be observed, thereby adaptively adjusting the position of the optical assembly 10 so that the optical assembly 10 can observe the region to be observed at the appropriate position. By defining the sliding direction of the focusing member 20 through the first adapter block 41, the possibility of deflection of the focusing member 20 can be reduced, thereby playing a role in maintaining the posture of the focusing member 20.

[0040] Referring to FIGS. 2 and 3, according to one or more embodiments of the present application, the first adapter block 41 can be provided with a receiving groove 411. The extension direction of the receiving groove 411 is parallel to the spacing direction between the sample plate and the optical assembly 10. The main body portion 200 is slidably disposed in the receiving groove 411. In this case, the receiving groove 411 can play a role in reducing the possibility of uneven deflection of the focusing member 20, thereby facilitating the maintenance of the posture of the focusing member 20 to be substantially constant. In addition, this assembly relationship of the focusing member 20 and the first adapter block 41 is also conducive to the replacement of the focusing member 20.

[0041] Referring to FIGS. 3 and 4, according to one or more embodiments of the present application, the main body portion 200 includes a top wall 2001 and a side wall 2002. The observation window 202 is provided in the top wall 2001. The side wall 2002 is slidably inserted into the receiving groove 411. The hollow region enclosed by the observation window 202 and the side wall 2002 is in communication. The side wall 2002 can be a continuous structure, for example, in the form of a cylinder, or can be a discontinuous structure, for example, including a plurality of support walls arranged at intervals. The optical assembly 10 can observe the sample plate through the hollow region enclosed by the side wall 2002 and the observation window 202. In addition, the side wall 2002 encloses a hollow region, and the side wall 2002 is in the form of a cylinder or includes a plurality of support walls arranged at intervals, which also improves the stability when the side wall 2002 and the optical assembly 10 abut, so that the side wall 2002 and / or the top wall 2001 are more difficult to deflect relative to the optical assembly 10.

[0042] Referring to FIGS. 2 and 3, the active focusing member 32 includes a driving motor 321, a transmission screw 322, and a screw nut 323. The extension direction of the transmission screw 322 is parallel to the spacing direction between the sample plate and the optical assembly 10. The optical assembly 10 and the screw nut 323 are relatively fixed. The optical assembly 10 can be directly disposed in the screw nut 323 or indirectly fixed to the screw nut 323 through other adapter structures. The driving motor 321 can drive the transmission screw 322 to rotate to move the screw nut 323 and the optical assembly 10. When the active focusing member 32 drives the optical assembly 10 to move, the relative position between the optical assembly 10 and the passive focusing member 31 changes.

[0043] Referring to FIG. 2 and FIG. 3, the follow-up focusing component 31 includes a mounting block 311 and an abutting block 312 fixed opposite to the mounting block 311, the abutting block 312 is slidably arranged on the mounting frame 40 and can abut against the fixed focusing component 20 in the follow-up focusing mode, and the active focusing component 32 is arranged on the mounting block 311. When the imaging assembly 1 is in the follow-up focusing mode, the abutting block 312 abuts against the fixed focusing component 20 and is fixed opposite to the active focusing component 32 and movable relative to the mounting frame 40. The sliding direction of the abutting block 312 can be the spacing direction of the optical assembly 10 and the sample plate, and can be arranged on the mounting frame 40 by a motor-screw, a slider-rail, a slider-slot or the like structure, which is not specifically limited herein. In this case, when the imaging assembly 1 is in the follow-up focusing mode, the focusing assembly 30 as a whole can adaptively move following the fixed focusing component 20. When the imaging assembly 1 is in the debugging mode, the abutting block 312 abuts against the fixed focusing component 20 and is fixed relative to the mounting frame 40, and the active focusing component 32 actively adjusts the position of the optical assembly 10. When the imaging assembly 1 is in the active focusing mode, the abutting block 312 does not abut against the fixed focusing component 20, and the abutting block 312 is movable relative to the mounting frame 40 to adjust the position of the focusing assembly 30 and the optical assembly 10 relative to the sample plate.

[0044] In addition, referring to FIG. 2 and FIG. 3, the focusing assembly 30 further includes an elastic component 33. The elastic component 33 is arranged between the mounting frame 40 and the follow-up focusing component 31, and is used to apply an action to the follow-up focusing component 31 to make it abut against the fixed focusing component 20.

[0045] For example, in some embodiments, the sample plate needs to be placed below the optical assembly 10 for observation, and the fixed focusing component 20 is correspondingly located below the optical assembly 10. In this case, the optical assembly 10 can abut against the fixed focusing component 20 under the action of gravity, and the fixed focusing component 20 can abut against the sample plate. The elastic component 33 can provide an upward action force to the optical assembly 10, which can be less than the gravity of the optical assembly 10, so as to reduce the possibility of damage to the sample plate caused by the full action of the gravity of the optical assembly 10.

[0046] For another example, the sample plate can also be placed above the optical assembly 10 for observation, and the fixed focusing component 20 is correspondingly located above the optical assembly 10. The elastic component 33 can provide an upward action force to the optical assembly 10, which can be greater than the gravity of the optical assembly 10, so that the optical assembly 10 can overcome the gravity and abut against the fixed focusing component 20 under the action of the elastic component 33, and further make the fixed focusing component 20 abut against the sample plate.

[0047] Referring to FIG. 4 and FIG. 5, according to one or more embodiments of the present application, the contact bump 201 is configured to abut any two points within a region of the sample plate to the same distance from a preset axis. The preset axis can pass through the optical center or focal point of the objective lens 11, and is perpendicular to the direction of the interval between the first bump 201a and the second bump 201b. In this case, no matter which region of the sample plate abuts against the contact bump 201, the distance from the sample plate to the objective lens 11 can always be maintained relatively constant.

[0048] In other embodiments, the contact bump 201 is configured to abut any two points within a region of the sample plate to a distance from a preset axis that differs by less than 0.1 mm, 0.2 mm, 0.3 mm, 0.4 mm, 0.5 mm, 0.6 mm, 0.7 mm, 0.8 mm, 0.9 mm, 1 mm, 1.5 mm, or 2 mm.

[0049] The contact area between the contact bump 201 and the sample plate is small, and when the contact bump 201 is unevenly stressed, or when there is a foreign object between the focusing member 20 and the optical assembly 10, a slight deflection (e.g., 0.5 degrees or 0.3 degrees) can occur. Through the above-mentioned manner, even if the region of the sample plate that is in contact with the contact bump 201 changes when the contact bump 201 rotates, the distance from the region of the sample plate that is being observed to the objective lens 11 can still be substantially within the appropriate range.

[0050] Referring to FIG. 2 and FIG. 6, according to one or more embodiments of the present application, the imaging device 1 can further include a carrying platform 50. The carrying platform 50 can be disposed on the mounting frame 40. In addition, the carrying platform 50 can have a placement region 501 for placing a sample plate. The sample plate can be inserted into the placement region 501. For example, the placement region 501 can be a fixed groove 502 formed in the carrying platform 50. The sample plate can be inserted into the fixed groove 502. In other embodiments, the placement region 501 can also not be in the form of a groove.

[0051] In some embodiments, the carrying platform 50 can be located above the optical assembly 10. The carrying platform 50 can further include an opening 503 that is in communication with the fixed groove 502. The opening 503 can be formed on the side of the carrying platform 50 that faces the focusing member 20, so that the focusing member 20 can abut against the sample plate inside the fixed groove 502 via the opening 503. The area of the opening 503 can be smaller than the area of the fixed groove 502 and the sample plate, so as to reduce the possibility of the sample plate falling out of the opening 503.

[0052] In some embodiments, when the sample plate is not inserted, the optical assembly 10 and the focusing assembly 30 may, under the action of gravity or the elastic member 33, force the fixed focus member 20 to abut against the bearing platform 50. In this case, when the user inserts the sample plate into the bearing platform 50, the sample plate may collide with the fixed focus member 20 during the movement of the sample plate driven by the bearing platform 50, affecting the user experience, and even possibly affecting the sample.

[0053] Referring to FIGS. 7 and 8, the side of the bearing platform 50 facing the fixed focus member 20 is provided with a transition bump 51. The main body portion 200 has a first region 203 and a second region 204 arranged along the movement direction of the bearing platform 50. The spacing between the first region 203 and the bearing platform 50 is smaller than the spacing between the second region 204 and the bearing platform 50.

[0054] The transition bump 51 is configured such that, during the insertion of the sample plate into the placement region 501 and the movement of the sample plate driven by the bearing platform 50, the projection of the transition bump 51 falls into the first region 203 and the second region 204 in sequence. When the projection of the transition bump 51 falls into the first region 203, the transition bump 51 abuts against the first region 203 of the fixed focus member 20. When the projection of the transition bump 51 falls into the second region 204, the projection of the contact bump 201 falls into the placement region 501.

[0055] In this case, when the user inserts the sample plate into the bearing platform 50 and moves the sample plate, the transition bump 51 can first abut against the first region 203, so that the contact bump 201 of the fixed focus member 20 and the bearing platform 50 form a spacing that can allow the sample plate to enter, and then, after the projection of the transition bump 51 falls into the second region 204, the contact bump 201 abuts against the sample plate. In this way, the risk of collision between the sample plate and the fixed focus member 20 during the movement of the sample plate driven by the bearing platform 50 can be effectively reduced.

[0056] According to one or more embodiments of the present application, the bearing platform 50 further comprises a transition region 205 connected between the first region 203 and the second region 204, the transition region 205 being smoothly connected with the first region 203 and smoothly connected with the second region 204. For example, in the direction from the first region 203 to the transition region 205, the curvature of the transition region 205 first increases and then decreases. In the direction from the second region 204 to the transition region 205, the curvature of the transition region 205 first increases and then decreases. In this way, the jerk during the movement of the sample plate driven by the bearing platform 50 can be reduced, the user experience can be improved, and the collision of the fixed focus member 20 with the sample plate can be reduced.

[0057] According to one or more embodiments of the present application, the imaging device 1 can be a fluorescent cell counter. The object to be observed can be a sample plate. The sample plate has a cell sample thereon, which can be stained by fluorescent dye and emit fluorescence under the action of an excitation light source. The imaging device 1 can collect the fluorescent signal and output corresponding picture information based on the fluorescent signal. Of course, the imaging device 1 of the present application can also not be limited to a cell counter.

[0058] Finally, it should be noted that: the above embodiments are mainly used to illustrate the technical solutions of the present application, and should not be understood as a limitation of the present application; the foregoing embodiments have been described in detail and specifically, and a person of ordinary skill in the art can modify the technical solutions recorded in the foregoing embodiments, or replace part or all of the technical features; and these modifications or replacements cannot make the corresponding technical solutions and the technical solutions of the present application constitute different inventions, and thus should be covered in the scope of the claims and the specification of the present application. Especially, in the absence of structural conflicts or combination barriers, each technical feature mentioned in each embodiment can be combined in any way, and these combinations still essentially should not be considered as deviating from the scope of the technical solutions of the present application.

Claims

1. An image forming apparatus characterized by comprising: The imaging device comprises: an optical assembly, a focusing member, a focusing assembly, and a mounting frame; the focusing member is movably arranged on the mounting frame, the focusing assembly comprises a following focusing member and a driving focusing member, and the optical assembly is arranged on the driving focusing member; the imaging device has a following focusing mode, in which the focusing member always abuts against a region to be observed on a sample plate, the following focusing member always abuts against the focusing member and moves synchronously with the focusing member, and the optical assembly is relatively fixed with the following focusing member, so that the distance between the optical assembly and the region to be observed on the sample plate is relatively constant; in other modes, the driving focusing member can drive the optical assembly to move independently relative to the focusing member and / or the following focusing member, so as to adjust the distance of the optical assembly to the sample plate or the relative position between the optical assembly and the focusing member.

2. The imaging device according to claim 1, wherein the focusing member comprises a main body portion and a contact protrusion arranged on the main body portion; the main body portion is provided with an observation window, and the contact protrusion comprises first and second protrusions arranged on both sides of the observation window and protruding away from the optical assembly.

3. The imaging device according to claim 2, wherein the contact protrusion is configured to abut against any two points in the region on the sample plate, and the distance of the two points to a preset axis is the same, or the difference between the distances of any two points in the region on the sample plate to the preset axis is less than 0.1 mm, 0.2 mm, 0.3 mm, 0.4 mm, 0.5 mm, 0.6 mm, 0.7 mm, 0.8 mm, 0.9 mm, 1 mm, 1.5 mm, or 2 mm; the optical assembly comprises an objective lens arranged close to the focusing member, the preset axis passes through the optical center or focal point of the objective lens, and is parallel to the interval direction of the first and second protrusions.

4. The imaging device according to claim 1, wherein the imaging device further comprises a bearing platform, the bearing platform has a placement region, and a sample plate can be inserted into the placement region; a transition protrusion is arranged on the side of the bearing platform facing the focusing member, the focusing member has a first region and a second region arranged along the moving direction of the bearing platform, and the interval between the first region and the bearing platform is less than the interval between the second region and the bearing platform; the transition protrusion is configured such that, during the process of inserting the sample plate into the placement region and moving the sample plate by the bearing platform, the projection of the transition protrusion falls into the first region and the second region in sequence, and when the projection of the transition protrusion falls into the first region, the transition protrusion abuts against the first region of the focusing member.

5. The imaging device according to claim 4, wherein the bearing platform further comprises a transition region connected between the first region and the second region, the transition region is smoothly connected with the first region and the second region.

6. The imaging device according to claim 2, wherein The mounting frame has a first adapter block with a receiving slot, the extension direction of the receiving slot is parallel to the interval direction between the sample plate and the optical assembly, and the focusing member is slidably arranged in the receiving slot.

7. The imaging device of claim 6, wherein, The main body part comprises a top wall and a side wall, the observation window is arranged in the top wall, the side wall is slidably arranged in the receiving slot, and the hollow region enclosed by the observation window and the side wall is in communication.

8. The imaging device of claim 1, wherein, The active focusing member comprises a driving motor, a transmission screw rod and a screw nut, the extension direction of the transmission screw rod is parallel to the interval direction between the sample plate and the optical assembly, the optical assembly and the screw nut are fixed relative to each other, and the driving motor is capable of driving the transmission screw rod to rotate so as to drive the screw nut to move the optical assembly.

9. The imaging device of claim 1, wherein, The follow-up focusing member comprises a mounting block and an abutting block fixed relative to the mounting block, the abutting block is slidably arranged in the mounting frame and is capable of abutting against the focusing member in the follow-up focusing mode, and the active focusing member is arranged in the mounting block.

10. The imaging device of claim 1, wherein, The focusing assembly further comprises an elastic member arranged between the mounting frame and the follow-up focusing member, and used for applying an action to the follow-up focusing member so as to abut against the focusing member.

Images