Nucleic acid integrated testing apparatus based on flexible structure and push-pull structure

Abstract

The present application relates to the technical field of nucleic acid testing. Disclosed is a nucleic acid integrated testing apparatus based on a flexible structure and a push-pull structure, the apparatus comprising a testing main body, an upper cover, a bottom housing, and a squeezing block provided between the upper cover and the bottom housing; the testing main body is provided in an accommodating cavity between the upper cover and the bottom housing; the testing main body comprises the following elements connected in sequence: a sample injection tube, a nucleic acid extraction-free reagent chamber, a first buffer solution chamber, a nucleic acid amplification reagent chamber, a second buffer solution chamber, and a uniform mixing chamber, all of which are flexible reagent chambers, as well as a sample loading recess and a chromatography test strip; a head end of the sample loading recess is connected to the uniform mixing chamber, and a tail end of the sample loading recess is connected to the chromatography test strip; and the squeezing block can move from one end of the upper cover towards the other end, and is used for squeezing a liquid in the testing main body to force the liquid to flow downstream. Because the liquid is squeezed to flow downstream only when the squeezing block is manually pushed to move, controllability of the testing process can be achieved.

Description

Nucleic acid integrated detection device based on flexible structure and push-pull structure

[0001] Cross-reference to related applications

[0002] The present application claims priority to the Chinese patent application No. 202411274884.X, filed on September 11, 2024, and entitled “Nucleic acid integrated detection device based on flexible structure and push-pull structure”, the entire content of which is incorporated herein by reference. TECHNICAL FIELD

[0003] The present application relates to the technical field of nucleic acid detection, in particular to a nucleic acid integrated detection device based on flexible structure and push-pull structure. BACKGROUND

[0004] Molecular diagnostic technology plays a very important role in pathogen detection, among which nucleic acid detection is the most reliable and direct method, and plays an important role in epidemic prevention and control and pathogen tracing. The conventional nucleic acid detection technology mainly refers to fluorescent quantitative PCR, but this process must rely on large-scale instruments and equipment, and laboratory personnel with professional knowledge reserve, so it limits its application in instant nucleic acid detection.

[0005] In addition, in recent years, due to the expansion of detection demand and the richness of detection scene, the rapid development of instant nucleic acid detection has been promoted. The existing instant nucleic acid detection equipment will continue to proceed until the detection result is displayed once the detection starts. However, the detection personnel will inevitably be interrupted during the detection process, and when they return to the detection site, the detection result may no longer be accurate due to the long time interval.

[0006] Therefore, it is necessary to provide a new detection device to overcome the above-mentioned defects. SUMMARY

[0007] The purpose of the present application is to provide a nucleic acid integrated detection device based on flexible structure and push-pull structure with controllable detection process.

[0008] To solve the above technical problems, the technical solution of the present application is as follows:

[0009] A nucleic acid integrated detection device based on flexible structure and push-pull structure, comprising a detection main body, an upper cover, a bottom shell, and a pressing block installed between the upper cover and the bottom shell.

[0010] The detection body is arranged in a containing cavity between the upper cover and the bottom shell; the detection body comprises, in sequence, a sample inlet tube, a nucleic acid extraction-free reagent chamber, a first buffer chamber, a nucleic acid amplification reagent chamber, a second buffer chamber, a mixing chamber, a sample loading groove and chromatographic test paper; the nucleic acid extraction-free reagent chamber, the first buffer chamber, the nucleic acid amplification reagent chamber, the second buffer chamber and the mixing chamber are all flexible reagent chambers; a first end of the sample loading groove is connected to the mixing chamber, and a second end of the sample loading groove is connected to the chromatographic test paper; wherein

[0011] The extrusion block is movable from one end of the upper cover to the other end, for extruding liquid in the detection body and forcing the liquid to flow downstream.

[0012] Optionally, two adjacent flexible reagent chambers are divided into two independent chambers by a weld seam, and one-third of the length of the weld seam is a virtual weld seam.

[0013] Optionally, the upper cover is provided with an upper sliding groove, the bottom shell is provided with a partition wall, the extrusion block comprises, in sequence, a handle, a neck portion and an extrusion head, the handle protrudes from the top surface of the upper cover, the neck portion slides along the upper sliding groove, and the extrusion head is located between the partition wall and the long side wall of the bottom shell.

[0014] Optionally, a side sliding groove is recessed inwardly on the side of the partition wall facing the extrusion head, and the extrusion head slides along the side sliding groove.

[0015] Optionally, a limiting protrusion is arranged on the neck portion, and a limiting structure is recessed in the side wall of the upper sliding groove corresponding to the first buffer chamber, and the limiting protrusion cooperates with the limiting structure.

[0016] Optionally, the detection device further comprises a U-shaped buckle, an opening of the buckle faces the neck portion and is clamped on the neck portion, and a bottom surface of the buckle abuts against the top surface of the upper cover, so that the sliding block does not extrude the liquid in the detection body.

[0017] Optionally, the sample loading groove is arranged parallel to the short side wall of the bottom shell, the chromatographic test paper is arranged parallel to the long side wall of the bottom shell, and a detection result display window is arranged on the upper cover corresponding to the chromatographic test paper.

[0018] Optionally, a first end of the sample inlet tube is clamped on a short side wall of the bottom shell and protrudes from the short side wall, or a first end of the sample inlet tube is clamped on the top surface of the upper cover and protrudes from the top surface of the upper cover.

[0019] Optionally, the sample inlet tube is connected to the nucleic acid extraction-free reagent chamber in sequence through a rigid pipeline and a flexible pipeline, and the rigid pipeline is arranged obliquely towards the bottom wall of the bottom shell.

[0020] Optionally, two heating modules are further included, and the two heating modules are respectively used for heating the nucleic acid extraction-free reagent chamber and the nucleic acid amplification reagent chamber.

[0021] The technical scheme has the following advantages:

[0022] 1. The nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided in the application comprises a detection main body, an upper cover, a bottom shell, and a pressing block arranged between the upper cover and the bottom shell; the detection main body is arranged in a containing cavity between the upper cover and the bottom shell; the detection main body comprises, in sequence, a sample inlet tube, a nucleic acid extraction-free reagent chamber, a first buffer solution chamber, a nucleic acid amplification reagent chamber, a second buffer solution chamber, a mixing chamber, a sample loading groove, and chromatographic test paper; the nucleic acid extraction-free reagent chamber, the first buffer solution chamber, the nucleic acid amplification reagent chamber, the second buffer solution chamber, and the mixing chamber are all flexible reagent chambers; a first end of the sample loading groove is connected with the mixing chamber, and a tail end of the sample loading groove is connected with the chromatographic test paper; wherein the pressing block moves from one end of the upper cover to the other end, is used for pressing liquid in the detection main body, and forces the liquid to flow downstream, so that the liquid is pressed to flow downstream only when the pressing block is manually pushed to move, and the controllability of the detection process is realized.

[0023] 2. The nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided in the application, two adjacent flexible reagent chambers are divided into two independent chambers by a welding seam, and one-third of the length of the welding seam is a virtual welding seam, so that the independence of the two adjacent flexible reagent chambers can be ensured, and the two adjacent flexible reagent chambers can be smoothly connected when the pressing block presses the flexible reagent chamber, and the smooth detection is ensured.

[0024] 3. The nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided in the application, a limiting protrusion is arranged on a neck portion of the pressing block, the limiting protrusion is matched with a clamping structure arranged on a side wall of an upper sliding groove and corresponding to the first buffer solution chamber, the limiting protrusion can be used for prompting that the pressing block has been slid to the position, and the controllability in the detection process is further improved.

[0025] 4. The nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided in the application, the detection device further comprises a U-shaped buckle, an opening of the buckle faces the neck portion and is clamped on the neck portion, a bottom surface of the buckle abuts against a top surface of the upper cover, so that the sliding block cannot produce a pressing effect on the liquid in the detection main body, and the setting of the buckle can avoid the unexpected pressing of the detection main body by the pressing block and avoid the scrapping of the detection main body due to the mispressing of the pressing block.

[0026] 5. The nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided in the application, the sample loading groove is arranged parallel to the short side wall of the bottom shell, the chromatographic test paper is arranged parallel to the long side wall of the bottom shell, and the detection result display window is arranged on the upper cover corresponding to the chromatographic test paper, so that the size of the bottom shell can be fully utilized, and the volume of the detection device is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the specific embodiments or prior art of the present application, the drawings needed to be used in the description of the specific embodiments or prior art will be briefly introduced. Obviously, the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0028] Fig. 1 is a perspective view of the detection device in the embodiment of the present application;

[0029] Fig. 2 is a perspective view of the detection column in the embodiment of the present application;

[0030] Fig. 3 is a perspective view of the upper cover in the embodiment of the present application;

[0031] Fig. 4 is a perspective view of the bottom shell in the embodiment of the present application;

[0032] Fig. 5 is a perspective view of the first angle of the extrusion block in the embodiment of the present application;

[0033] Fig. 6 is a perspective view of the second angle of the extrusion block in the embodiment of the present application;

[0034] Fig. 7 is a perspective view of the cooperation between the buckle and the extrusion block in the embodiment of the present application;

[0035] Fig. 8 is a perspective view of the buckle in the embodiment of the present application;

[0036] Fig. 9 is a schematic view of the internal weld of the mixing chamber in the embodiment of the present application.

[0037] Explanation of reference signs: 1, detection main body; 11, sealing cover; 12, sample inlet tube; 121, rigid pipeline; 122, flexible pipeline; 13, nucleic acid extraction-free reagent chamber; 14, first buffer chamber; 15, nucleic acid amplification reagent chamber; 16, second buffer chamber; 17, mixing chamber; 18, sample loading groove; 178, flexible bag pipeline; 171, virtual welding seam; 172, real welding seam; 19, chromatographic test paper; 2, upper cover; 21, result display window; 22, upper sliding groove; 23, clamping structure; 3, bottom shell; 31, partition wall; 32, side sliding groove; 33, first heating plate storage groove; 34, first battery storage groove; 35, first switch button; 36, second heating plate storage groove; 37, second battery storage groove; 38, second switch button; 39, test strip support; 391, test strip fixing member; 310, sample inlet tube limiting hole; 312, first display lamp; 313, second display lamp; 4, extrusion block; 41, handle; 42, neck; 421, limiting protrusion; 44, extrusion head; 5, buckle. DETAILED DESCRIPTION

[0038] The technical solutions of the present application will be described clearly and completely below in combination with the drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0039] In the description of the present application, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0040] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0041] In addition, the technical features involved in the different embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.

[0042] As shown in FIGS. 1-9, the present application provides a nucleic acid integrated detection device based on flexible structure and push-pull structure (hereinafter referred to as detection device), which mainly comprises a detection main body 1, an upper cover 2, a bottom shell 3, a squeezing block 4 and a buckle 5. The detection main body 1 is placed in the accommodating cavity between the bottom shell 3 and the upper cover 2.

[0043] The detection main body 1 comprises a sample inlet tube 12, a nucleic acid extraction-free reagent chamber 13, a first buffer chamber 14, a nucleic acid amplification reagent chamber 15, a second buffer chamber 16, a mixing chamber 17, a sample loading groove 18 and a chromatographic test paper 19 connected in sequence. Since the nucleic acid extraction-free reagent chamber 13, the first buffer chamber 14, the nucleic acid amplification reagent chamber 15 and the second buffer chamber 16 are chambers made of flexible material, they are collectively referred to as flexible reagent chambers. The number of flexible reagent chambers can be determined according to the required reaction steps and functions.

[0044] In the view of FIG. 2, the sample inlet tube 12 is arranged perpendicularly to the short side wall on the left side of the bottom shell 3 for injecting the sample, and the outer end of the sample inlet tube 12 is threadedly connected with the sealing cover 11 and sealed by the sealing cover 11. As shown in FIG. 2, the end of the sample inlet tube 12 located in the bottom shell 3 is connected with a rigid pipeline 121 inclined downward toward the bottom of the bottom shell 3 to reduce the residue of the reagent in the rigid pipeline 121. The end of the rigid pipeline 121 away from the sample inlet tube 12 is connected with a flexible pipeline 122 so that the sample can be squeezed into the flexible reagent chamber by the squeezing block 4. The pipeline connected with the end of the sample inlet tube 12 located in the bottom shell 3 can also be a soft pipeline. In this case, the length of the soft pipeline is relatively large, which can be used for liquid samples as well as swab samples, and the sample loss can be reduced by squeezing the soft pipeline with the swab. Before sample addition, the sample inlet tube 12 can be placed in a vertical upward state by changing the orientation of the entire device, which is beneficial for the sample to enter the flexible reagent chamber smoothly in addition to facilitating sample addition.

[0045] The buffer solution in the first buffer chamber 14 is the buffer solution in the corresponding nucleic acid amplification system in the nucleic acid amplification reagent chamber 15. The nucleic acid amplification reagent chamber 15 can use RPA, LAMP and other isothermal amplification technologies, or PCR variable temperature amplification technology. The volume of the nucleic acid amplification reagent chamber 15 is twice the volume of the liquid therein, so as to make the nucleic acid amplification system as flat as possible, increase the contact area with the heating plate described below, and make the liquid evenly heated. The reagent in the second buffer chamber 16 can be water or other diluent, which is used to dilute the amplification product before reaching the chromatographic test paper 19 for detection.

[0046] The whole device can be turned upside down 2-3 times to achieve better mixing effect. The size of each flexible reagent chamber can be adjusted according to the volume of different reagents, but the uniform length of the weld between the adjacent two flexible reagent chambers should be ensured, and the width of the weld should be reserved to avoid affecting the volume of each flexible reagent chamber. The length of 1 / 3 of each weld between the adjacent two flexible reagent chambers is welded by virtual welding technology, and the length of 2 / 3 is welded by ordinary hot welding technology. It should be noted that the liquid pressure in the upper flexible reagent chamber can be increased to break the virtual weld part downstream, thereby realizing the mixing of the liquids in the adjacent two flexible reagent chambers, and the real weld is not affected in the process. The flexible reagent chamber is made of flexible material, which can be polypropylene, polyethylene, polyurethane, polyolefin copolymer, etc.

[0047] In the view of FIG. 2, the front end of the sample slot 18 is connected to the mixing chamber 17 through the flexible bag pipe 178 (the flexible bag pipe 178 is essentially an extension of the mixing chamber 17), and a chromatographic test paper 19 for detection is arranged at the tail end of the sample slot 18. In the view of FIG. 1, the sample slot 18 is arranged parallel to the short side wall on the right side of the bottom shell 3. The chromatographic test paper 19 is arranged parallel to the long side wall of the bottom shell 3. The lower surface of the sample slot 18 is fixed to the inner bottom surface of the bottom shell 3 by double-sided adhesive to prevent it from shaking during transportation of the detection main body 1. The detection result is directly presented by the chromatographic test paper 19, and the same system multiple detection can be realized by using different primer probe markers.

[0048] The upper cover 2 is provided with a result display window 21, an upper sliding groove 22 for sliding of the neck portion 42 of the following extrusion block 4, and a clamping structure 23 (in this embodiment, the clamping structure 23 is a circular groove, and in other embodiments, it can also be an elongated groove) matched with the limiting protrusion 421 on the extrusion block 4. The clamping structure 23 is arranged corresponding to the first buffer chamber 14. The width of the upper sliding groove 22 is consistent with the width dimension at the neck portion 42, so as to avoid left and right shaking of the extrusion block 4 during sliding. The limiting protrusion 421 on the extrusion block 4 enters the pre-set clamping structure 23, which can remind the operator that the extrusion block 4 has been slid to the pre-set position, and the extrusion block 4 does not need to be pushed to continue moving to the right to control the reaction process.

[0049] The bottom shell 3 is provided with a partition wall 31. The partition wall 31 is inwardly recessed on the side facing the extrusion head 44 to form a side sliding groove 32. The extrusion head 44 of the following extrusion block 4 slides along the side sliding groove 32 between the partition wall 31 and a long side wall of the bottom shell 3. The side sliding groove 32 is in the shape of “L” and has a height consistent with the height of the extrusion head 44 of the extrusion block 4.

[0050] The bottom shell 3 is provided with a first heating plate storage slot 33 for storing the first heating plate, a first battery storage slot 34 for supplying power to the first heating plate, a first switch button 35, and a first display lamp 312. The first heating plate storage slot 33 is arranged directly below the nucleic acid extraction-free reagent chamber 13.

[0051] The bottom shell 3 is further provided with a second heating plate storage slot 36 for storing the second heating plate, a second battery storage slot 37 for supplying power to the second heating plate, a second switch button 38, and a second display lamp 313. The second heating plate storage slot 36 is arranged directly below the nucleic acid amplification reagent chamber 15.

[0052] Of course, the aforementioned heating plate can also be replaced by a warm patch or a self-heating bag. For the convenience of description, the heating plate, the warm patch, and the self-heating bag are collectively referred to as a heating module. When the heating module is a warm patch or a self-heating bag, the battery is not required to be arranged. In addition, whether the heating module is arranged depends on whether the reaction system has a heating requirement. When the heating module is not arranged, the first heating plate storage slot 33, the second heating plate storage slot 36, the first battery storage slot 34, and the second battery storage slot 37 can be reserved. Of course, when the reaction system does not have a heating requirement, the first heating plate storage slot 33, the second heating plate storage slot 36, the first battery storage slot 34, and the second battery storage slot 37 can also not be arranged.

[0053] The bottom shell 3 is further provided with a test strip bracket 39. The test strip bracket 39 is provided with a plurality of test strip fixing members 391 for fixing the chromatographic test strip 19, so as to prevent the chromatographic test strip 19 from being displaced during transportation or use.

[0054] The bottom shell 3 is further provided with a sample inlet tube limiting hole 310 arranged on the short side wall adjacent to the sample inlet tube 12. The sample inlet tube limiting hole 310 is used for fixing the sample inlet tube 12 of the detection main body 1. The inner diameter of the sample inlet tube limiting hole 310 is equivalent to the outer diameter of the sample inlet tube 12.

[0055] As shown in FIGS. 5 and 6, as an embodiment, the pressing block 4 includes a handle 41, a neck 42, and a pressing head 44 connected in sequence. The handle 41 can be of any shape and always protrudes from the top surface of the upper cover 2. The neck 42 is provided with a limiting protrusion 421 on one side. The limiting protrusion 421 cooperates with the following-described clamping structure 23. The pressing head 44 is used to press the flexible reagent chamber, so as to make the upstream flexible reagent chamber communicate with the downstream flexible reagent chamber. Specifically, the pressing head 44 is a thin wedge-shaped head in the form of a straight line. In the width direction of the flexible reagent chamber, the width of the pressing head 44 at least completely covers the flexible reagent chamber. The width value of the pressing head 44 is equivalent to the spacing value between the corresponding side sliding groove 32 and the long side wall of the bottom shell 3, so as to ensure that the pressing block 4 can drive the liquid in the corresponding flexible reagent chamber to enter the downstream flexible reagent chamber completely and without position deviation during the sliding process.

[0056] As shown in FIG. 7 and FIG. 8, the buckle 5 is in U shape, and the opening of the buckle 5 faces the neck 42. The width of the buckle 5 is greater than the width of the upper slide groove 22 and less than the width of the handle 41, so that the buckle 5 can be clamped on the neck 42 of the pressing block 4 and cannot be separated upward from the pressing block 4. During transportation or before detection, the bottom surface of the buckle 5 abuts against the top surface of the upper cover 2, so that the pressing head 44 of the pressing block 4 does not contact or only slightly contacts the flexible pipeline 122 and the flexible reagent chamber below. When it is needed to make the pressing head 44 press the flexible pipeline 122 and the flexible reagent chamber, the buckle 5 is removed, and the pressing block 4 is pressed downward to the bottom of the side slide groove 32, and then the pressing block 4 is slid. The first heating plate and the second heating plate are collectively referred to as a heating plate.

[0057] As shown in FIG. 9, the mixing chamber 17 is provided with two virtual welding seams 171 at two ends respectively, and the virtual welding seams 171 are opened only under the pressing of the pressing block 4. A plurality of solid welding seams 172 are staggered between the two virtual welding seams 171, so as to form a Z-shaped mixing flow channel in the mixing chamber 17, which is used to improve the mixing effect.

[0058] The specific operation process of the nucleic acid integrated detection device based on the flexible structure and the push-pull structure in the embodiment is as follows:

[0059] The detection device is stood up, the sample inlet tube 12 is vertically upward, the sample is added, and the sealing cover 11 is tightly screwed. At this time, the sample flows into the nucleic acid extraction-free reagent chamber 13 and is mixed with the nucleic acid extraction-free reagent.

[0060] The buckle 5 is removed, and the pressing block 4 is pressed toward the bottom of the bottom shell 3 to abut the bottom of the pressing head 44 against the flexible pipeline 122, so that the sample in the flexible pipeline 122 is squeezed into the nucleic acid extraction-free reagent chamber 13.

[0061] The detection device is placed horizontally. Due to the blocking of the pressing block 4, the mixed liquid in the nucleic acid extraction-free reagent chamber 13 cannot flow back to the direction of the sample inlet tube 12.

[0062] The first switch button 35 is opened, the first display lamp 312 is turned on, the first heating plate heats the nucleic acid extraction-free reagent chamber 13 to a preset temperature, and the sample starts to be lysed until the first display lamp 312 is turned off.

[0063] The handle 41 is held to push the pressing block 4 to move away from the sample inlet tube 12. In the process of sliding the pressing block 4, the lysed solution in the nucleic acid extraction-free reagent chamber 13 and the RPA buffer in the first buffer chamber 14 enter the nucleic acid amplification reagent chamber 15 and are mixed with the RPA reagent in the nucleic acid amplification reagent chamber 15. When the limiting protrusion 421 on the pressing block 4 coincides with the clamping structure 23 and a jamming feeling occurs, the sliding of the pressing block 4 is stopped.

[0064] Turning on the second switch button 38, the second display lamp 313 is lighted, the second heating plate heats the nucleic acid amplification reagent chamber 15, the sample in the nucleic acid amplification reagent chamber 15 is subjected to an amplification reaction, until the second display lamp 313 is turned off, and the amplification is completed to obtain an amplification product;

[0065] Continuously pushing the extrusion block 4 away from the sample tube 12 by holding the handle 41, the amplification product flows through the second buffer chamber 16, is mixed and diluted with the diluent in the second buffer chamber 16, is subjected to mixing in the mixing chamber 17, and finally reaches the chromatographic test paper 19 via the flexible bag tube 178, the sample loading groove 18, and is detected by the chromatographic test paper 19, and the detection result is output by the result display window 21.

[0066] The nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided in the application adopts a unique chamber isolation and fluid control technology, and realizes rapid, simple and low-cost nucleic acid detection. Specifically, the device utilizes the real weld and the virtual weld to construct different functional chambers and mixing flow channels in the detection main body 1, and the real weld can ensure the sealing and isolation between the chambers, and the virtual weld provides a controllable propulsion connection mechanism, allowing the virtual weld to be forced to open by physical means (by extrusion of the extrusion block 4) when needed, so as to realize the communication between adjacent chambers.

[0067] In addition, the nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided in the application adopts a slider extrusion mechanism (in this embodiment, the physical extrusion of the extrusion block 4 on the chamber promotes the flow of liquid), so that the reaction process can be controlled. The advantage of this design is its simplicity and directness, without relying on external pumping systems or complex valve control. The implementation of the slider extrusion mechanism promotes the continuous flow of liquid in the microchannel and between the chambers by applying mechanical force to a specific area of the flexible reagent chamber, ensuring uniform mixing and rapid transmission of reactants. This design not only simplifies the fluid control mechanism, reduces the manufacturing cost and operation difficulty of the device, but also expands the use environment of the detection system, making it more suitable for use in resource-limited areas or on-site rapid detection scenarios.

[0068] In addition, the design of the nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided in the application fully considers the user experience and operation simplicity, so that non-professionals can easily perform nucleic acid detection operations, and due to its small size and portability, it can be applied to various application scenarios (including clinical laboratories, on-site detection, home self-detection, etc.), greatly expanding the accessibility and application range of nucleic acid detection.

[0069] Obviously, the above embodiments are merely example for clearly illustrating but not limitation to the embodiments. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. Here, all the embodiments are not required to be enumerated. The obvious changes or variations derived from the above description are still within the protection scope of the present application.

Claims

1. A nucleic acid integration detection device based on a flexible structure and a push-pull structure, characterized by, The detection device comprises a detection body (1), an upper cover (2), a bottom shell (3), and a pressing block (4) arranged between the upper cover (2) and the bottom shell (3). The detection body (1) is arranged in a containing cavity between the upper cover (2) and the bottom shell (3); the detection body (1) comprises, in sequence, a sample inlet tube (12), a nucleic acid extraction-free reagent chamber (13), a first buffer chamber (14), a nucleic acid amplification reagent chamber (15), a second buffer chamber (16), a mixing chamber (17), a sample loading groove (18), and chromatographic test paper (19); the nucleic acid extraction-free reagent chamber (13), the first buffer chamber (14), the nucleic acid amplification reagent chamber (15), the second buffer chamber (16), and the mixing chamber (17) are all flexible reagent chambers; a first end of the sample loading groove (18) is connected to the mixing chamber (17), and a second end of the sample loading groove (18) is connected to the chromatographic test paper (19). The pressing block (4) is movable from one end to the other end of the upper cover (2) to press the liquid in the detection body (1) and force the liquid to flow downstream.

2. The nucleic acid integration detection device based on the flexible structure and the push-pull structure according to claim 1, characterized in that, Two adjacent flexible reagent chambers are divided into two independent chambers by a weld seam, and one-third of the length of the weld seam is a virtual weld seam.

3. The nucleic acid integration detection device based on the flexible structure and the push-pull structure according to claim 1, characterized in that, The upper cover (2) is provided with an upper sliding groove (22), the bottom shell (3) is provided with a partition wall (31), the pressing block (4) comprises, in sequence, a handle (41), a neck portion (42), and a pressing head (44), the handle (41) protrudes from the top surface of the upper cover (2), the neck portion (42) is slidable along the upper sliding groove (22), and the pressing head (44) is located between the partition wall (31) and the long side wall of the bottom shell (3).

4. The nucleic acid integration detection device based on the flexible structure and the push-pull structure according to claim 3, characterized in that, The partition wall (31) is recessed inward on the side facing the pressing head (44) and is provided with a side sliding groove (32), and the pressing head (44) is slidable along the side sliding groove (32).

5. The nucleic acid integration detection device based on the flexible structure and the push-pull structure according to claim 3, characterized in that, The side wall of the upper sliding groove (22) is recessed and provided with a clamping structure (23) corresponding to the first buffer chamber (14), the neck portion (42) is provided with a limiting protrusion (421), and the limiting protrusion (421) is clamped with the clamping structure (23).

6. The nucleic acid integration detection device based on the flexible structure and the push-pull structure according to claim 3, characterized in that, The detection device further comprises a U-shaped buckle (5), the opening of the buckle (5) faces the neck portion (42) and is clamped on the neck portion (42), the bottom surface of the buckle (5) abuts against the top surface of the upper cover (2), so that the pressing block (4) cannot exert a pressing force on the liquid in the detection body (1).

7. The flexible structure and pull structure based nucleic acid integrated detection device according to any one of claims 1-6, characterized in that, The sample loading groove (18) is arranged parallel to the short side wall of the bottom shell (3), the chromatographic test paper (19) is arranged parallel to the long side wall of the bottom shell (3), and the upper cover (2) is provided with a detection result display window (21) corresponding to the chromatographic test paper (19).

8. The nucleic acid integration detection device based on the flexible structure and the push-pull structure according to claim 7, characterized in that, A first end of the sample inlet tube (12) is clamped on a short side wall of the bottom shell (3) and protrudes from the short side wall, or a first end of the sample inlet tube (12) is clamped on the top surface of the upper cover (2) and protrudes from the top surface of the upper cover (2).

9. The nucleic acid integration detection device based on the flexible structure and the push-pull structure according to claim 8, characterized in that, The sample injection tube (12) is connected with the nucleic acid extraction-free reagent chamber (13) through a rigid pipe (121) and a flexible pipe (122) in sequence, and the rigid pipe (121) is arranged obliquely towards the bottom wall of the bottom shell (3).

10. The flexible structure and push-pull structure based nucleic acid integrated detection device according to any one of claims 1-6, 8-9, wherein, Two heating modules are further included, and the two heating modules are respectively used for heating the nucleic acid extraction-free reagent chamber (13) and the nucleic acid amplification reagent chamber (15).

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