Rotary structure-based universal automated nucleic acid extraction module

Abstract

The present application relates to the technical field of nucleic acid testing, and discloses a rotary structure-based universal automated nucleic acid extraction module, comprising a liquid storage layer and a flow channel layer which are vertically arranged. The nucleic acid extraction module is internally provided with a lysis buffer chamber, a binding buffer chamber, a magnetic bead chamber, a front wash buffer chamber, a rear wash buffer chamber, an elution buffer chamber, a sample processing chamber, a waste liquid chamber, and a sample loading chamber. A valve core is mounted in the sample processing chamber; a sample processing chamber piston is mounted in a core cavity of the valve core; and a side wall of the core cavity is provided with a liquid transfer port in communication with the core cavity. The top surface of the flow channel layer is correspondingly provided with flow channels in communication with the lysis buffer chamber, the binding buffer chamber, the magnetic bead chamber, the front wash buffer chamber, the rear wash buffer chamber, the elution buffer chamber, the waste liquid chamber, and the sample loading chamber, respectively; and the valve core rotates around its own axis and can be in communication with different flow channels. The switching of the liquid flow channels is convenient, the structure is simpler, the assembly is easier, the cost is lower, the volume is smaller, and the weight is lighter.

Description

Universal automated nucleic acid extraction module based on rotating structure

[0001] Cross-reference to related applications

[0002] The present application claims priority to the Chinese patent application No. 202411274848.3, filed on September 11, 2024, and entitled "Universal automated nucleic acid extraction module based on rotating structure", the entire content of which is incorporated herein by reference. TECHNICAL FIELD

[0003] The present application relates to the technical field of nucleic acid detection, in particular to a universal automated nucleic acid extraction module based on rotating structure. BACKGROUND

[0004] Nucleic acid detection occupies an extremely important position in pathogen detection due to its high accuracy, sensitivity and specificity, and it is still used as the gold standard for clinical diagnosis.

[0005] Nucleic acid extraction methods include cell lysis method, centrifugal column method, magnetic bead method, etc., among which the magnetic bead method is to modify and modify the surface of magnetic nanoparticles, so that they can reversibly bind and release nucleic acids under certain conditions. The magnetic bead method has the characteristics of simple operation, short time consumption, and can realize automation and high-throughput operation, and the obtained nucleic acid has high purity and large concentration.

[0006] It is impossible to meet the market demand for high-throughput by manually preparing reagents during the nucleic acid extraction process. Traditional nucleic acid extraction devices often need multiple valves (especially microvalves) to control liquid flow, and a highly integrated valve system can lead to a more complex overall structure of the device and increased cost. In order to accommodate a large number of microvalves and their control circuits, the volume and weight of the device are often difficult to reduce, which limits its deployment and movement in a limited laboratory environment.

[0007] Therefore, it is urgent to improve the traditional nucleic acid extraction device to overcome the above-mentioned defects. SUMMARY

[0008] The purpose of the present application is to provide a universal automated nucleic acid extraction module based on rotating structure with simple liquid flow control, so as to reduce the number of valves and the volume and weight of the nucleic acid extraction module.

[0009] To solve the above technical problems, the technical solution of the present application is as follows:

[0010] A universal automated nucleic acid extraction module based on rotating structure, the nucleic acid extraction module comprising a liquid storage layer and a flow channel layer arranged in sequence from top to bottom;

[0011] The nucleic acid extraction module is internally provided with a lysis liquid chamber, a binding liquid chamber, a magnetic bead chamber, a pre-washing liquid chamber, a post-washing liquid chamber, an elution liquid chamber, a sample processing chamber, a waste liquid chamber, and a sample loading chamber.

[0012] The sample processing chamber extends upward through the top surface of the liquid storage layer and downward into the flow channel layer, the sample processing chamber is internally provided with a valve core, the valve core is internally provided with a sample processing chamber piston, and a liquid transfer port communicating with the core cavity is formed on the side wall of the core cavity.

[0013] The top surface of the flow channel layer is provided with flow channels communicating with the sample processing chamber corresponding to the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the pre-washing liquid chamber, the post-washing liquid chamber, the elution liquid chamber, the waste liquid chamber, and the sample loading chamber.

[0014] The valve core can rotate around its axis to make the liquid transfer port communicate with different flow channels.

[0015] Optionally, a vortex flow channel is formed on the inner bottom wall of the core cavity of the valve core.

[0016] Optionally, the magnetic bead chamber is located between the binding liquid chamber and the sample processing chamber, and the flow channel corresponding to the binding liquid chamber extends through the flow channel corresponding to the magnetic bead chamber.

[0017] Optionally, the flow channels corresponding to the lysis liquid chamber, the binding liquid chamber, the pre-washing liquid chamber, the post-washing liquid chamber, and the elution liquid chamber are radially arranged from one end of a main flow channel, and the other end of the main flow channel communicates with the sample processing chamber.

[0018] Optionally, the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the pre-washing liquid chamber, the post-washing liquid chamber, and the elution liquid chamber each extend upward through the top surface of the liquid storage layer, and the nucleic acid extraction module further comprises a flexible membrane layer arranged on the bottom wall of the liquid storage layer, the flexible membrane layer is used to seal the bottom ends of the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the pre-washing liquid chamber, the post-washing liquid chamber, and the elution liquid chamber in the liquid storage layer.

[0019] Optionally, each of the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the pre-washing liquid chamber, the post-washing liquid chamber, and the elution liquid chamber is internally provided with a rubber plug, and each bottom wall is provided with a thorn-like structure for piercing the corresponding part of the flexible membrane layer.

[0020] Optionally, the nucleic acid extraction module is further provided with a pressure buffer chamber extending through the top surface of the liquid storage layer and the bottom surface of the flow channel layer, and a rubber plug is arranged in the pressure buffer chamber and can float up and down in the pressure buffer chamber.

[0021] Optionally, the nucleic acid extraction module further comprises a gasket layer between the flexible film layer and the liquid storage layer, and through holes are arranged on the gasket layer vertically corresponding to the pressure buffer chamber, the lysis solution chamber, the binding solution chamber, the magnetic bead chamber, the pre-washing solution chamber, the post-washing solution chamber, the elution solution chamber, the sample processing chamber, the waste liquid chamber and the sample loading chamber.

[0022] Optionally, the waste liquid chamber extends through the top surface of the liquid storage layer and into the flow channel layer, and a waste liquid chamber piston is arranged in the waste liquid chamber.

[0023] Optionally, the sample loading chamber extends through the top surface of the liquid storage layer and the bottom surface of the flow channel layer, and a sample loading chamber piston is arranged in the sample loading chamber.

[0024] Optionally, the nucleic acid extraction module further comprises an electromagnet arranged at the bottom of the sample processing chamber, the electromagnet can rotate around its axis, and the valve core can rotate synchronously with the electromagnet.

[0025] The technical scheme has the following advantages:

[0026] 1. The universal automatic nucleic acid extraction module based on a rotating structure provided by the present application, wherein the sample processing chamber extends through the top surface of the liquid storage layer and into the flow channel layer, a valve core is arranged in the sample processing chamber, a sample processing chamber piston is arranged in the core cavity of the valve core, and a liquid transfer port is arranged on the side wall of the core cavity and communicates with the core cavity; the top surface of the flow channel layer is provided with flow channels corresponding to the lysis solution chamber, the binding solution chamber, the magnetic bead chamber, the pre-washing solution chamber, the post-washing solution chamber, the elution solution chamber, the waste liquid chamber and the sample loading chamber, wherein the valve core can rotate around its axis to connect the liquid transfer port with different flow channels, so that the sample processing chamber can be connected with different chambers by rotating the valve core to obtain different liquid flow paths. Compared with arranging valves (especially micro-valves) corresponding to each chamber, the structure is simpler, the assembly is simpler, the cost is lower, the volume is smaller and the weight is lighter.

[0027] 2. The universal automatic nucleic acid extraction module based on a rotating structure provided in the present application, the inner bottom wall of the valve cavity of the valve core is designed as a vortex flow channel, which can cause sufficient disturbance of the liquid flowing into the valve core to suspend the magnetic beads at the bottom of the valve core, thereby ensuring that the nucleic acid is fully washed and effectively eluted, and also helping to reduce non-specific binding, ensuring high purity of nucleic acid extraction.

[0028] 3. The universal automatic nucleic acid extraction module based on a rotating structure provided in the present application, the magnetic bead chamber is located between the binding liquid chamber and the sample processing chamber, and the flow channel corresponding to the binding liquid chamber extends through the flow channel corresponding to the magnetic bead chamber. In this way, when the liquid is transferred, the binding liquid will flow through the flow channel through which the magnetic beads flow, so as to further flush the flow channel corresponding to the magnetic bead chamber, thereby reducing the residue of the magnetic beads in the flow channel corresponding to the magnetic bead chamber and improving the detection accuracy.

[0029] 4. The universal automatic nucleic acid extraction module based on a rotating structure provided in the present application, the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the front washing liquid chamber, the rear washing liquid chamber, and the eluent chamber are respectively provided with a rubber plug, and the bottom wall of each chamber is provided with a thorn-shaped structure for piercing the corresponding position on the flexible film layer. The rubber plug can not only push the flexible film layer towards the thorn-shaped structure to pierce the flexible film layer, but also further squeeze the liquid in each chamber to reduce the residue of the liquid in each chamber.

[0030] 5. The universal automatic nucleic acid extraction module based on a rotating structure provided in the present application, the nucleic acid extraction module further comprises a gasket layer located between the flexible film layer and the liquid storage layer, and the gasket layer is vertically provided with through holes corresponding to the pressure buffer chamber, the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the front washing liquid chamber, the rear washing liquid chamber, the eluent chamber, the sample processing chamber, the waste liquid chamber and the sample loading chamber. The gasket layer provides more downward deformation space for the flexible film layer, ensuring that the thorn-shaped structure can pierce the flexible film layer.

[0031] 6. The universal automatic nucleic acid extraction module based on a rotating structure provided in the present application, the waste liquid chamber extends through the top surface of the liquid storage layer upwardly and extends into the flow channel layer downwardly, and the waste liquid chamber is provided with a waste liquid chamber piston. When the waste liquid enters the waste liquid chamber, the waste liquid chamber piston is lifted upwardly to accelerate the speed of the waste liquid entering the waste liquid chamber from the sample processing chamber, and to buffer the pressure in the device.

[0032] 7. The universal automatic nucleic acid extraction module based on a rotating structure provided in the present application, the sample loading chamber extends through the top surface of the liquid storage layer upwardly and extends through the bottom surface of the flow channel layer downwardly, and the sample loading chamber is provided with a sample loading chamber piston. During sample loading, the sample loading chamber piston can promote the liquid to move downstream to accelerate the sample loading. BRIEF DESCRIPTION OF DRAWINGS

[0033] In order to more clearly illustrate the technical solutions in the specific embodiments or the prior art of the present application, the drawings needed to be used in the description of the specific embodiments or the prior art will be briefly introduced. Obviously, the drawings in the following description are some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without any creative effort.

[0034] Fig. 1 is a perspective assembly view of the nucleic acid providing module in the embodiments of the present application;

[0035] Fig. 2 is a perspective exploded view of the nucleic acid providing module in the embodiments of the present application;

[0036] Fig. 3 is a perspective view of the rubber plug and the piston of the nucleic acid providing module in the embodiments of the present application, both of which expose the top surface of the liquid storage layer;

[0037] Fig. 4 is a perspective view of the valve core in the embodiments of the present application;

[0038] Fig. 5 is an internal view of the valve core in the embodiments of the present application;

[0039] Fig. 6 is a top view of the valve core in the embodiments of the present application;

[0040] Fig. 7 is a perspective view of the flow channel layer in the embodiments of the present application.

[0041] Legend of reference signs: 1, liquid storage layer; 10, pressure buffer chamber; 100, buffer chamber rubber plug; 11, lysis liquid chamber; 111, first rubber plug; 12, binding liquid chamber; 121, second rubber plug; 13, magnetic bead chamber; 131, third rubber plug; 14, pre-washing liquid chamber; 141, fourth rubber plug; 15, post-washing liquid chamber; 151, fifth rubber plug; 16, elution liquid chamber; 161, sixth rubber plug; 17, sample processing chamber; 170, valve core; 171, sample processing chamber piston; 172, sealing member; 173, clamping column; 174, liquid transfer port; 175, vortex type flow channel; 18, waste liquid chamber; 181, waste liquid chamber piston; 19, sample loading chamber; 191, sample loading chamber piston; 2, flexible film layer; 3, gasket layer; 4, flow channel layer; 40, main flow channel; 42, electromagnet; 421, clamping groove; 43, first flow channel; 44, second flow channel; 45, third flow channel; 46, fourth flow channel; 47, fifth flow channel; 48, waste liquid flow channel; 49, sample loading flow channel; A, thorn-like structure. DETAILED DESCRIPTION

[0042] The technical solutions of the present application will be described clearly and completely below with reference to the drawings. Obviously, the described embodiments are some of the embodiments of the present application, but not all the embodiments of the present application. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0043] In the description of the present application, it should be noted that the terms "upper", "lower", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second", "third", "fourth", "fifth", "sixth" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0044] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0045] In addition, the technical features involved in the different embodiments of the present application described below can be combined with each other as long as there is no conflict.

[0046] As shown in FIGS. 1-7, the present application provides a universal automatic nucleic acid extraction module based on a rotating structure (hereinafter referred to as the nucleic acid extraction module). The nucleic acid extraction module comprises a liquid storage layer 1 and a flow channel layer 4. The nucleic acid extraction module is provided with a lysis solution chamber 11 (also used as a sample chamber) for storing a lysis solution, a binding solution chamber 12 for storing a binding solution, a magnetic bead chamber 13 for storing magnetic beads, a front washing solution chamber 14 for storing a washing solution, a rear washing solution chamber 15 for storing a washing solution, an elution solution chamber 16 for storing an elution solution, a sample processing chamber 17, a waste liquid chamber 18 for receiving waste liquid generated during the reaction, and a sample loading chamber 19 for collecting the final nucleic acid elution solution. The sample processing chamber 17 extends upward through the top surface of the liquid storage layer 1 and downward through the bottom wall of the flow channel layer 4. The sample processing chamber 17 is provided with a valve core 170, the core cavity of the valve core 170 is provided with a sample processing chamber piston 171, and the side wall of the core cavity is provided with a liquid transfer port 174 communicating with the core cavity. The top surface of the flow channel layer 4 is provided with flow channels corresponding to the lysis solution chamber 11, the binding solution chamber 12, the magnetic bead chamber 13, the front washing solution chamber 14, the rear washing solution chamber 15, the elution solution chamber 16, the waste liquid chamber 18, and the sample loading chamber 19, respectively, which communicate with the sample processing chamber 17. The valve core 170 can rotate around its own axis to make the liquid transfer port 174 communicate with different flow channels.

[0047] In the present application, the sample processing chamber 17 extends upward through the top surface of the liquid storage layer 1 and downward through the bottom wall of the flow channel layer 4, the sample processing chamber 17 is provided with a valve core 170, the core cavity of the valve core 170 is provided with a sample processing chamber piston 171, and the side wall of the core cavity is provided with a liquid transfer port 174 communicating with the core cavity; the top surface of the flow channel layer 4 is provided with flow channels corresponding to the lysis solution chamber 11, the binding solution chamber 12, the magnetic bead chamber 13, the front washing solution chamber 14, the rear washing solution chamber 15, the elution solution chamber 16, the waste liquid chamber 18, and the sample loading chamber 19, respectively, which communicate with the sample processing chamber 17 (as shown in FIG. 7), wherein the valve core 170 can rotate around its own axis to make the liquid transfer port 174 communicate with different flow channels, so that the communication between the sample processing chamber 17 and different chambers (such as the lysis solution chamber 11, the binding solution chamber 12, the magnetic bead chamber 13, the front washing solution chamber 14, the rear washing solution chamber 15, the elution solution chamber 16, the waste liquid chamber 18, and the sample loading chamber 19) can be realized by rotating the valve core 170, different liquid flow paths can be obtained to complete nucleic acid extraction, compared with setting valves (especially micro-valves) corresponding to each chamber, the structure is simpler, the assembly is simpler, the cost is lower, the volume is smaller, and the weight is lighter.

[0048] Optionally, a seal 172 (as shown in FIG. 4, the seal 172 is a seal ring, and the number can be two, of course, the number can also be other values) is arranged on the outer wall of the valve core 170, which can ensure the sealing of the sample processing chamber 17 when the valve core 170 rotates. In this embodiment, the bottom end face of the valve core 170 is a closed end face.

[0049] Optionally, as shown in FIGS. 4-6, the inner bottom wall of the valve cavity of the valve core 170 is designed with a vortex flow channel 175, so that the liquid flowing into the valve core 170 can be disturbed to suspend the magnetic beads in the bottom of the valve core 170, thereby ensuring that the nucleic acid is fully washed and effectively eluted, and also helping to reduce non-specific binding, ensuring high purity of nucleic acid extraction.

[0050] Optionally, the height of the liquid transfer port 174 is the same as the height of the flow channel, and the bottom of the liquid transfer port 174 is basically flush with the bottom wall of the flow channel.

[0051] Optionally, the shape of the cross section of the flow channel can be semicircular but is not limited to semicircular.

[0052] Optionally, the magnetic bead chamber 13 is located between the binding liquid chamber 12 and the sample processing chamber 17, and the flow channel corresponding to the binding liquid chamber 12 and the flow channel corresponding to the magnetic bead chamber 13 are arranged in a line, so that when the liquid is transferred, the binding liquid will flow through the flow channel through which the magnetic beads flow, so as to further flush the flow channel corresponding to the magnetic bead chamber 13, thereby reducing the residue of the magnetic beads in the flow channel corresponding to the magnetic bead chamber 13.

[0053] Optionally, as shown in FIG. 7, the flow channels corresponding to the lysis liquid chamber 11, the binding liquid chamber 12, the pre-washing liquid chamber 14, the post-washing liquid chamber 15, and the elution liquid chamber 16 are arranged in a radial manner from one end of a main flow channel 40, and the other end of the main flow channel 40 is connected to the sample processing chamber 17. For easy understanding, the flow channels corresponding to the lysis liquid chamber 11, the pre-washing liquid chamber 14, the post-washing liquid chamber 15, and the elution liquid chamber 16 are respectively referred to as the first flow channel 43, the third flow channel 45, the fourth flow channel 46, and the fifth flow channel 47. In this embodiment, since the flow channels corresponding to the binding liquid chamber 12 and the magnetic bead chamber 13 are arranged in a line, they are collectively referred to as the second flow channel 44. The flow channel between the sample processing chamber 17 and the waste liquid chamber 18 is referred to as the waste liquid flow channel 48, and the flow channel between the sample processing chamber 17 and the sample loading chamber 19 is referred to as the sample loading flow channel 49.

[0054] Optionally, the lysis solution chamber 11, the binding solution chamber 12, the magnetic bead chamber 13, the pre-washing solution chamber 14, the post-washing solution chamber 15, and the elution solution chamber 16 extend upward through the top surface of the liquid storage layer 1, and the nucleic acid extraction module further comprises a flexible film layer 2 arranged on the bottom wall of the liquid storage layer 1, and the flexible film layer 2 is used to seal the bottom ends of the lysis solution chamber 11, the binding solution chamber 12, the magnetic bead chamber 13, the pre-washing solution chamber 14, the post-washing solution chamber 15, and the elution solution chamber 16 in the liquid storage layer 1.

[0055] Optionally, each of the lysis solution chamber 11, the binding solution chamber 12, the magnetic bead chamber 13, the pre-washing solution chamber 14, the post-washing solution chamber 15, and the elution solution chamber 16 is provided with a rubber plug, and each of the bottom walls is provided with a thorn-like structure A for piercing the corresponding part of the flexible film layer 2. Specifically, the diameter of the rubber plug is equal to the diameter of the corresponding chamber, so as to improve the sealing performance of the detection process. In this embodiment, the rubber plug is a silica gel plug.

[0056] For the convenience of understanding, the rubber plugs in the lysis solution chamber 11, the binding solution chamber 12, the magnetic bead chamber 13, the pre-washing solution chamber 14, the post-washing solution chamber 15, and the elution solution chamber 16 are respectively referred to as the first rubber plug 111, the second rubber plug 121, the third rubber plug 131, the fourth rubber plug 141, the fifth rubber plug 151, and the sixth rubber plug 161.

[0057] Optionally, the nucleic acid extraction module further comprises a pressure buffer chamber 10 extending upward through the top surface of the liquid storage layer 1 and downward through the bottom surface of the flow channel layer 4, and the pressure buffer chamber 10 is provided with a rubber plug (referred to as a buffer chamber rubber plug 100) which can float up and down in the pressure buffer chamber 10. The pressure buffer chamber 10 is used to buffer the pressure in the downstream amplification device or detection device during the sample loading process.

[0058] Optionally, the nucleic acid extraction module further comprises a gasket layer 3 between the flexible film layer 2 and the liquid storage layer 1, and the gasket layer 3 is provided with through holes vertically corresponding to the pressure buffer chamber 10, the lysis solution chamber 11, the binding solution chamber 12, the magnetic bead chamber 13, the pre-washing solution chamber 14, the post-washing solution chamber 15, the elution solution chamber 16, the sample processing chamber 17, the waste liquid chamber 18, and the sample loading chamber 19. In this embodiment, the gasket layer 3 provides more downward deformation space for the flexible film layer 2, ensuring that the thorn-like structure A can pierce the flexible film layer 2.

[0059] Optionally, the waste liquid chamber 18 extends upward through the top surface of the liquid storage layer 1 and downward into the flow channel layer 4, and the waste liquid chamber 18 is provided with a waste liquid chamber piston 181. The waste liquid chamber piston 181 can be lifted to accelerate the speed of the waste liquid from the sample processing chamber 17 into the waste liquid chamber 18. By pulling up the waste liquid chamber piston 181, the waste liquid can be transferred into the waste liquid chamber 18, and the waste liquid chamber 18 can also serve as an air chamber to buffer the pressure inside the device during the nucleic acid extraction process.

[0060] Optionally, the material of the flexible film layer 2 includes but is not limited to TPU, polyethylene, polypropylene, etc. The bonding method of the flexible film layer 2 and the liquid storage layer 1 includes but is not limited to double-sided adhesive, hot melt or ultrasonic welding.

[0061] Optionally, the sample loading chamber 19 extends upward through the top surface of the liquid storage layer 1 and downward through the bottom surface of the flow channel layer 4, and the sample loading chamber 19 is provided with a sample loading chamber piston 191. During sample loading, the sample loading chamber piston 191 can promote the downward movement of the liquid to accelerate the sample loading.

[0062] Optionally, the nucleic acid extraction module further comprises an electromagnet 42 arranged at the bottom of the sample processing chamber 17, the electromagnet 42 can rotate around its axis, and the valve core 170 can rotate synchronously with the electromagnet 42. Specifically, the top of the electromagnet 42 is provided with a clamping groove 421, and the bottom of the valve core 170 is provided with a clamping column 173, the clamping column 173 is clamped with the clamping groove 421, so that the electromagnet 42 drives the valve core 170 to rotate synchronously. The electromagnet 42 can also attract magnetic beads, and the bottom end of the electromagnet 42 is connected to a rotating device, and the rotating device is used to drive the electromagnet 42 to rotate.

[0063] The specific operation process of the general type of automatic nucleic acid extraction module provided in the present application is as follows:

[0064] The valve core 170 is rotated to make the liquid transfer port 174 communicate with the first flow channel 43, a syringe is inserted through the first rubber plug 111, the sample is added into the lysis liquid chamber 11, and the first rubber plug 111 is pressed to the bottom of the lysis liquid chamber 11, so that the sample and the lysis liquid enter the first flow channel 43, and the sample processing chamber piston 171 in the sample processing chamber 17 is extracted upward, and the mixed liquid is transferred to the sample processing chamber 17 for lysis reaction;

[0065] After the lysis reaction is completed, the electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 communicates with the second flow channel 44, the second rubber plug 121 and the third rubber plug 131 are pressed to the bottom of the binding liquid chamber 12 and the magnetic bead chamber 13 respectively, and the sample processing chamber piston 171 is extracted, so that the binding liquid and the magnetic beads are transferred into the valve core 170 for binding reaction;

[0066] During the reaction process, the electromagnet 42 outside the bottom of the valve core 170 will attract the magnetic beads to the inner bottom surface of the valve core 170;

[0067] The electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the waste liquid flow channel 48. The electromagnet 42 is stopped, the sample processing chamber piston 171 is pressed down, and the waste liquid chamber piston 181 is lifted up, so that the waste liquid is transferred to the waste liquid chamber 18;

[0068] After the waste liquid is transferred, the electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the third flow channel 45. The electromagnet 42 is stopped;

[0069] The fourth rubber plug 141 is pressed down, and the sample processing chamber piston 171 is extracted, so that the washing liquid in the front washing liquid chamber 14 is transferred to the sample processing chamber 17 for washing. During the washing process, the electromagnet 42 no longer attracts the magnetic beads to the inner bottom surface of the valve core 170, so that the nucleic acid is fully washed;

[0070] The electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the waste liquid flow channel 48. At the same time, the electromagnet 42 is started to attract the magnetic beads to the inner bottom surface of the valve core 170. The sample processing chamber piston 171 is pressed down, and the waste liquid chamber piston 181 is lifted up, so that the waste liquid is transferred to the waste liquid chamber 18;

[0071] After the waste liquid is transferred, the electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the fourth flow channel 46;

[0072] The electromagnet 42 is stopped, the fifth rubber plug 151 is pressed down, and the sample processing chamber piston 171 is extracted, so that the washing liquid is transferred to the sample processing chamber 17 for washing;

[0073] During the washing process, the electromagnet 42 is disconnected, and the magnetic beads are no longer attracted to the inner bottom surface of the valve core 170, so that the nucleic acid is fully washed;

[0074] The electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the waste liquid flow channel 48. At the same time, the electromagnet 42 is started to attract the magnetic beads to the inner bottom surface of the valve core 170;

[0075] The electromagnet 42 is stopped, the sample processing chamber piston 171 is pressed down, and the waste liquid chamber piston 181 is lifted up, so that the waste liquid is transferred to the waste liquid chamber 18;

[0076] After the waste liquid is transferred, the electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the fifth flow channel 47;

[0077] Stop rotating the electromagnet 42, and disconnect the electromagnet 42, no longer adsorb the magnetic beads to the inner bottom surface of the valve core 170, so that the nucleic acid is fully eluted;

[0078] Press the sixth rubber plug 161 downward, and extract the sample processing chamber piston 171, to transfer the eluent into the sample processing chamber 17 for elution;

[0079] After elution is completed, the electromagnet 42 adsorbs the magnetic beads to the inner bottom surface of the valve core 170; and rotates the electromagnet 42 to drive the valve core 170 to rotate, so that the liquid transfer port 174 is communicated with the sample flow channel 49;

[0080] Stop rotating the electromagnet 42, press the sample processing chamber piston 171 downward, and pull the sample chamber piston 191 upward at the same time, to transfer the nucleic acid eluent into the sample chamber 19;

[0081] Rotate the electromagnet 42 to drive the valve core 170 to rotate, so that the liquid transfer port 174 is misaligned with the sample flow channel 49, and press the sample chamber piston 191 to complete the sample loading process in the downstream device.

[0082] In the process of assembling the nucleic acid extraction module, first, the rubber plug is inserted into the corresponding chamber, and the top end of the rubber plug does not protrude from the top surface of the liquid storage layer 1, then the nucleic acid extraction module is inverted, the rubber plug blocks the bottom end of the corresponding chamber, then the liquid is injected into the chamber, and the flexible film layer 2 is sealed to each chamber. Compared with the process of first arranging the flexible film layer 2 at the bottom of the liquid storage layer 1, then placing the liquid storage layer 1 vertically, then injecting the liquid into the chamber, and finally inserting the rubber plug, the deformation of the flexible film layer 2 caused by the process of inserting the rubber plug into the chamber can be avoided.

[0083] The application provides a universal automatic nucleic acid extraction module based on a rotating structure, which realizes accurate transfer of liquid between different channels by using a rotating mechanism, thereby significantly improving the automation degree and processing efficiency.

[0084] The application provides a universal automatic nucleic acid extraction module based on a rotating structure, and a vortex flow channel 175 is designed on the inner bottom wall of the valve cavity of the valve core 170, so that the liquid flowing into the valve core 170 can generate a vortex effect and sufficient disturbance to suspend the magnetic beads in the bottom of the valve core 170, reduce the deposition of the magnetic beads, and make the distribution of the magnetic beads in the liquid more uniform, thereby ensuring that the nucleic acid is fully washed and effectively eluted, and also helping to reduce non-specific binding and ensure high purity of nucleic acid extraction.

[0085] Obviously, the above embodiments are merely example for clearly illustrating but not limitation to the embodiments. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. Here, all the embodiments are not required to be enumerated. The obvious changes or variations derived from the above description are still within the protection scope of the present application.

Claims

1. A universal automated nucleic acid extraction module based on a rotating structure, characterized by, The nucleic acid extraction module comprises a liquid storage layer (1) and a flow channel layer (4) arranged in sequence from top to bottom. The nucleic acid extraction module is internally provided with a lysis liquid chamber (11), a binding liquid chamber (12), a magnetic bead chamber (13), a front washing liquid chamber (14), a rear washing liquid chamber (15), an elution liquid chamber (16), a sample processing chamber (17), a waste liquid chamber (18), and a sample loading chamber (19). The sample processing chamber (17) extends upward through the top surface of the liquid storage layer (1) and downward through the bottom wall of the flow channel layer (4), and a valve core (170) is arranged in the sample processing chamber (17), a sample processing chamber piston (171) is arranged in the core cavity of the valve core (170), and a liquid transfer port (174) communicating with the core cavity is formed in the side wall of the core cavity. The top surface of the flow channel layer (4) is provided with flow channels corresponding to the lysis liquid chamber (11), the binding liquid chamber (12), the magnetic bead chamber (13), the front washing liquid chamber (14), the rear washing liquid chamber (15), the elution liquid chamber (16), the waste liquid chamber (18), and the sample loading chamber (19), respectively. The valve core (170) can rotate around its axis to make the liquid transfer port (174) communicate with different flow channels.

2. The universal automated nucleic acid extraction module based on a rotating structure according to claim 1, wherein, A vortex flow channel (175) is formed on the inner bottom wall of the core cavity of the valve core (170).

3. The universal automated nucleic acid extraction module based on a rotating structure according to claim 1, wherein, The magnetic bead chamber (13) is located between the binding liquid chamber (12) and the sample processing chamber (17), and the flow channel corresponding to the binding liquid chamber (12) extends through the flow channel corresponding to the magnetic bead chamber (13).

4. The universal automated nucleic acid extraction module based on a rotating structure according to claim 3, wherein, The flow channels corresponding to the lysis liquid chamber (11), the binding liquid chamber (12), the front washing liquid chamber (14), the rear washing liquid chamber (15), and the elution liquid chamber (16) extend radially from one end of a main flow channel (40), and the other end of the main flow channel (40) communicates with the sample processing chamber (17).

5. The universal automated nucleic acid extraction module based on a rotating structure according to any one of claims 1-4, characterized in that, The lysis liquid chamber (11), the binding liquid chamber (12), the magnetic bead chamber (13), the front washing liquid chamber (14), the rear washing liquid chamber (15), and the elution liquid chamber (16) extend upward through the top surface of the liquid storage layer (1), and the nucleic acid extraction module further comprises a flexible membrane layer (2) arranged on the bottom wall of the liquid storage layer (1), which is used to seal the bottom ends of the lysis liquid chamber (11), the binding liquid chamber (12), the magnetic bead chamber (13), the front washing liquid chamber (14), the rear washing liquid chamber (15), and the elution liquid chamber (16) in the liquid storage layer (1).

6. The universal automated nucleic acid extraction module based on a rotating structure according to claim 5, wherein, The lysis solution chamber (11), the binding solution chamber (12), the magnetic bead chamber (13), the pre-washing solution chamber (14), the post-washing solution chamber (15), and the elution solution chamber (16) are each provided with a rubber plug, and the bottom wall of each chamber is provided with a thorn-shaped structure (A) for piercing the corresponding part of the flexible film layer (2).

7. The universal automated nucleic acid extraction module based on a rotating structure according to claim 6, wherein, The nucleic acid extraction module is also provided with a pressure buffer chamber (10) extending through the top surface of the liquid storage layer (1) and the bottom surface of the flow channel layer (4), and the pressure buffer chamber (10) is provided with a rubber plug that can float up and down in the pressure buffer chamber (10).

8. The universal automated nucleic acid extraction module based on a rotating structure according to claim 7, wherein, The nucleic acid extraction module further comprises a gasket layer (3) between the flexible film layer (2) and the flow channel layer (4), and the gasket layer (3) is provided with through holes corresponding to the pressure buffer chamber (10), the lysis solution chamber (11), the binding solution chamber (12), the magnetic bead chamber (13), the pre-washing solution chamber (14), the post-washing solution chamber (15), the elution solution chamber (16), the sample processing chamber (17), the waste liquid chamber (18), and the sample loading chamber (19).

9. The universal automated nucleic acid extraction module based on a rotating structure according to claim 8, wherein, The waste liquid chamber (18) extends through the top surface of the liquid storage layer (1) and into the flow channel layer (4), and the waste liquid chamber (18) is provided with a waste liquid chamber piston (181).

10. The universal automated nucleic acid extraction module based on a rotating structure according to claim 8, wherein, The sample loading chamber (19) extends through the top surface of the liquid storage layer (1) and the bottom surface of the flow channel layer (4), and the sample loading chamber (19) is provided with a sample loading chamber piston (191).

11. The universal automated nucleic acid extraction module based on a rotating structure according to any one of claims 1-4, 6-10, characterized in that, The nucleic acid extraction module further comprises an electromagnet (42) installed at the bottom of the sample processing chamber (17), and the electromagnet (42) can rotate around its axis, and the valve core (170) can rotate synchronously with the electromagnet (42).

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