Multifunctional transfer device

Abstract

The present application discloses a multifunctional transfer device, comprising a multifunctional transfer box and a mounting submodule used for mounting the multifunctional transfer box, wherein the multifunctional transfer box comprises a negative electrode module and a positive electrode module, a first guide rail is provided on the side surface of the negative electrode module away from the positive electrode module, and a second guide rail is provided on the side surface of the positive electrode module away from the negative electrode module; the mounting submodule comprises a first mounting frame, the first mounting frame comprises fixed side plates extending in a third direction, the fixed side plates are respectively located on two sides of the multifunctional transfer box, limiting rails are provided on the inner side walls of the fixed side plates, the limiting rails work in conjunction with the first guide rail and the second guide rail, and the multifunctional transfer box is inserted into the first mounting frame along a first direction. In the present application, when the multifunctional transfer box of the multifunctional transfer device is inserted into the mounting submodule, the limiting rails on the side plates of the mounting submodule work in conjunction with the guide rails on the positive and negative electrode modules to play the role of limiting and fixing the multifunctional transfer box.

Description

A multifunctional transfer apparatus Technical Field

[0001] This application relates to the field of biotechnology, and in particular to a multifunctional transfer apparatus. Background Technology

[0002] There are two main transfer methods: wet transfer and semi-dry transfer. Wet transfer involves immersing a sandwich of filter paper, membrane, and gel in buffer solution, while semi-dry transfer uses filter paper to absorb the buffer solution. Wet transfer typically requires more buffer solution and is more complex, but it is more adaptable, offers better temperature control, and is less prone to errors. In contrast, semi-dry transfer requires less buffer solution and is simpler to operate, but it is more susceptible to failure due to membrane drying. Currently, some transfer instruments on the market combine wet and semi-dry transfer, continuously replenishing buffer solution during the semi-dry transfer phase to create a wet transfer environment, thus achieving successful transfer. Technical issues

[0003] This application provides a multifunctional transfer film apparatus. When the multifunctional transfer box in this apparatus is inserted into the installation submodule, the limiting rail on the side plate of the installation submodule cooperates with the guide rail on the positive and negative electrode modules to limit and fix the multifunctional transfer box. Technical solutions

[0004] This application provides a multifunctional film transfer device, which includes a multifunctional film transfer box and an installation submodule for mounting the multifunctional film transfer box; the multifunctional film transfer device has a first direction, a second direction and a third direction, and the first direction, the second direction and the third direction intersect each other;

[0005] The multifunctional transfer box includes a negative electrode module and a positive electrode module. A first guide rail is provided on the side of the negative electrode module away from the positive electrode module, and a second guide rail is provided on the side of the positive electrode module away from the negative electrode module. The first guide rail and the second guide rail extend along the first direction.

[0006] The installation submodule includes a first installation frame, which includes a fixed side plate extending along a third direction. The fixed side plate is located on both sides of the multifunctional transfer box, and a limiting rail is provided on the inner wall of the fixed side plate. The limiting rail extends along a first direction and cooperates with the first guide rail and the second guide rail. The multifunctional transfer box is inserted into the first installation frame along the first direction.

[0007] In some embodiments, the negative electrode module forms grooved surfaces on both sides of the side away from the positive electrode module as the first guide rail; the positive electrode module forms grooved surfaces on both sides of the side away from the negative electrode module as the second guide rail.

[0008] In some embodiments, the fixed side plate forms a limiting track on the inner side wall that is spaced apart along a third direction, and the negative electrode module or the positive electrode module is mounted on the first mounting frame through the limiting track.

[0009] In some embodiments, the positive electrode module of the multifunctional transfer film box includes a second housing, and two liquid quick-connect connectors are provided on the side wall of the second housing; a liquid circulation system is provided on the side of the second housing away from the negative electrode module; the liquid circulation system includes a first inlet connected to a liquid quick-connect connector for liquid inlet and a first outlet connected to a liquid quick-connect connector for liquid outlet; the first inlet is located at the lower end of the second housing, and the first outlet is located at the upper end of the second housing.

[0010] In some embodiments, the first inlet and the first outlet are fitted with a connector, the connector having a right-angle mounting tube disposed on a mounting base, and one end of the right-angle mounting tube penetrating the second housing.

[0011] In some embodiments, the liquid circulation system includes a plurality of outlets, with a buffer tube connected between at least two outlets.

[0012] In some embodiments, the mounting submodule includes a fixing base plate extending along a third direction, the fixing base plate being provided with a fixing mechanism, the fixing mechanism including a connector assembly, and the liquid quick-connect connector on the multifunctional transfer box being connected to the connector assembly.

[0013] In some embodiments, the fixing mechanism includes:

[0014] A first fixing mechanism, the first fixing mechanism includes a first fixing base, the first fixing base is provided with a first through hole for the connector to pass through;

[0015] The second fixing mechanism includes a second fixing base, on which a second through hole is provided for the connector assembly to pass through, and the first fixing base is fixed to the second fixing base; the second fixing base is provided with a third through hole for the second fixing member to pass through; the diameter of the second fixing member is smaller than the diameter of the third through hole.

[0016] In some embodiments, the second fixing base is fixed to the fixing substrate by the second fixing member, and an adjustment element is provided between the second fixing base and the fixing substrate, with the two ends of the adjustment element abutting against the fixing substrate and the second fixing base respectively.

[0017] In some embodiments, the first fixing base protrudes in a direction away from the second fixing base to form a first boss, and the first boss and the second fixing base form a first cavity, the first cavity being used to accommodate one end of the connector assembly.

[0018] In some embodiments, the diameter of the first through hole gradually decreases along the thickness direction of the first fixing base.

[0019] In some embodiments, the connector assembly includes a first connector and a second connector, wherein the diameter of the first connector is smaller than the diameter of the second through hole, and the diameter of the second connector is larger than the diameter of the second through hole.

[0020] In some embodiments, the multifunctional transfer apparatus further includes an identification mechanism, which includes an identification element, a first identification site, and a second identification site; the first identification site is located on the positive electrode module, and the identification element is used to identify whether the positive electrode module and the negative electrode module are in place and to determine whether the module is faulty. Beneficial effects

[0021] This application provides a multifunctional transfer film apparatus, including a multifunctional transfer film box and an installation submodule for mounting the multifunctional transfer film box. The multifunctional transfer film box includes a negative electrode module and a positive electrode module. A first guide rail is provided on the side of the negative electrode module away from the positive electrode module, and a second guide rail is provided on the side of the positive electrode module away from the negative electrode module. The installation submodule includes a first mounting frame, which includes fixed side plates extending along a third direction. The fixed side plates are located on both sides of the multifunctional transfer film box, and a limiting rail is provided on the inner wall of the fixed side plate. The limiting rail cooperates with the first guide rail and the second guide rail. The multifunctional transfer film box is inserted into the first mounting frame along a first direction. When the multifunctional transfer film box of this application is inserted into the installation submodule, the limiting rail on the side plate of the installation submodule cooperates with the guide rail on the positive and negative electrode modules, thereby limiting and fixing the multifunctional transfer film box.

[0022] Attached Figure Description

[0023] Figure 1 is a schematic diagram of the fixed frame structure of the multifunctional transfer film apparatus in the embodiment of this application;

[0024] Figure 2 is a schematic diagram of the fixed frame and mounting sub-module structure of the multifunctional transfer film machine in the embodiment of this application;

[0025] Figure 3 is a schematic diagram of the installation sub-module structure of the multifunctional transfer film apparatus in the embodiment of this application;

[0026] Figure 4 is a schematic diagram of the multifunctional transfer box structure in an embodiment of this application;

[0027] Figure 5 is a schematic diagram of the multifunctional transfer box structure in an embodiment of this application;

[0028] Figure 6 is a schematic diagram of the multifunctional transfer box structure in an embodiment of this application;

[0029] Figure 7 is a schematic diagram of the negative electrode module structure of the multifunctional transfer film box in the embodiment of this application;

[0030] Figure 8 is a schematic diagram of the split structure of the negative electrode module of the multifunctional transfer film box in the embodiment of this application;

[0031] Figure 9 is a schematic diagram of the split structure of the positive electrode module of the multifunctional transfer film box in the embodiment of this application;

[0032] Figure 10 is a schematic diagram of the structure of the second housing in the positive electrode module in an embodiment of this application;

[0033] Figure 11 is a schematic diagram of the structure of the rubber diaphragm in an embodiment of this application;

[0034] Figure 12 is a schematic diagram of the structure of the rubber diaphragm in the embodiment of this application;

[0035] Figure 13 is a schematic diagram of the structure of the rubber diaphragm in an embodiment of this application;

[0036] Figure 14 is a schematic diagram of another type of rubber seal in an embodiment of this application;

[0037] Figure 15 is a schematic diagram of another type of rubber seal in an embodiment of this application;

[0038] Figure 16 is a schematic diagram of the identification mechanism in an embodiment of this application;

[0039] Figure 17 is a schematic diagram of the identification mechanism in an embodiment of this application;

[0040] Figure 18 is a schematic diagram of the liquid circulation system structure in an embodiment of this application;

[0041] Figure 19 is a schematic diagram of the liquid circulation system structure in an embodiment of this application;

[0042] Figure 20 is a schematic diagram of the liquid circulation system structure in an embodiment of this application;

[0043] Figure 21 is a schematic diagram of the fixing mechanism in an embodiment of this application;

[0044] Figure 22 is a schematic diagram of the fixing mechanism in an embodiment of this application;

[0045] Figure 23 is a schematic diagram of the fixing mechanism in an embodiment of this application;

[0046] Figure 24 shows an example of the transfer experiment results of the multifunctional transfer apparatus of this application. The best embodiment of the present invention

[0047] The following description, in conjunction with the accompanying drawings, further illustrates the scheme of this application.

[0048] The multifunctional transfer apparatus of this application has multiple uses, such as performing membrane transfer and staining. Furthermore, the multifunctional transfer apparatus module in the embodiments of this application integrates multiple modules, enabling membrane transfer and staining to be performed simultaneously in one instrument when multiple sets of experiments are conducted at the same time.

[0049] In some embodiments, the multifunctional transfer apparatus includes a multifunctional transfer box 1 and a mounting submodule 3 for mounting the multifunctional transfer box 1; the multifunctional transfer apparatus has a first direction X, a second direction Y, and a third direction Z, wherein the first direction X, the second direction Y, and the third direction Z are perpendicular or approximately perpendicular to each other. The first direction X in this application can also be transverse.

[0050] In some embodiments, the multifunctional transfer apparatus of this application includes several multifunctional transfer apparatus sub-modules, each multifunctional transfer apparatus sub-module including an installation sub-module 3 and a corresponding multifunctional transfer container 1, and each multifunctional transfer apparatus sub-module can operate independently. In some embodiments, the multifunctional transfer apparatus of this application is an integrated structure with several independently operating multifunctional transfer apparatus sub-modules.

[0051] In some embodiments, the multifunctional transfer film apparatus of this application includes a fixed frame 6. As shown in FIG1, the fixed frame 6 in the embodiment of this application includes a first base plate 601, and a plurality of first support seats 602 are provided on the first base plate 601. The first support seats 602 extend along a first direction X, and the number of first support seats 602 corresponds to the number of sub-modules of the multifunctional transfer film apparatus.

[0052] In some embodiments, the fixed frame 6 is provided with a first limiting plate 603 at the front end, and a first limiting groove 604 is provided at the top of the first limiting plate 603. When the installation submodule 3 is inserted into the fixed frame 6, the first limiting plate 603 and the first limiting groove 604 limit the position of the installation submodule 3.

[0053] As shown in Figure 2, in some embodiments, the fixed frame 6 includes a first top plate 605. In some embodiments, the first top plate 605 is provided with a plurality of first heat dissipation vents 6051, which are used for heat dissipation of the unit.

[0054] In some embodiments, the multifunctional transfer film machine includes a heat dissipation mechanism 7. As shown in FIG2, the heat dissipation mechanism 7 of this application includes a plurality of cooling fans, which improves the heat dissipation performance of the entire unit and enhances operational stability.

[0055] As shown in Figure 3, the installation submodule 3 of this embodiment includes a first installation frame 300. The first installation frame 300 includes two fixed side plates 301 extending along a third direction Z, and the inner sidewall of the fixed side plates 301 is provided with limiting rails 302 extending along a first direction X. Each fixed side plate 301 has two limiting rails 302 distributed vertically. The limiting rails 302 can both guide the insertion of the multifunctional transfer film box 1 into the first installation frame 300 and limit and fix the multifunctional transfer film box 1. The multifunctional transfer film device of this application, through structural improvement, adopts a horizontal insertion method for the transfer film box, which facilitates operation.

[0056] As shown in Figure 3, the first mounting frame 300 of this application further includes a second base plate 304, a second top plate 305 disposed opposite to the second base plate 304, and a fixing base plate 303. The fixing base plate 303 of this application extends vertically and is parallel to the plane formed by the Y-axis and Z-axis. The fixing base plate 303, the second base plate 304, the fixing side plates 301 located on both sides of the second base plate 304, and the second top plate 305 of this application together form a mounting cavity for the multifunctional transfer box 1. The multifunctional transfer box is inserted laterally (along the first direction X) into the first mounting frame 300 from the opening side.

[0057] In some embodiments, the side of the second base plate 304 is inserted into the first limiting groove 604, which serves to limit the position of the installation submodule 3.

[0058] In some embodiments, a limiting member 306 is provided on the side of the second top plate 305. The limiting member 306 penetrates the second top plate 305, and its bottom end passes through the second top plate 305. In some embodiments, the limiting member 306 is a ball bearing set screw. In some embodiments, the multifunctional transfer film box 1 has a groove on the corresponding side of the negative electrode module 100 or the positive electrode module 200 for engaging with the bottom end of the limiting member 306. When the multifunctional transfer film box 1 is inserted into the corresponding position of the mounting cavity, the ball bearing set screw engages in the groove on the side of the negative electrode module 100 or the positive electrode module 200, thus playing a positioning role. In some specific embodiments, the groove is provided on the side of the first housing 110 of the negative electrode module 100 for positioning.

[0059] As shown in Figures 4, 5, and 6, the multifunctional transfer film box 1 of this application includes a negative electrode module 100 and a positive electrode module 200. A first guide rail 111 is provided on the side of the negative electrode module 100 away from the positive electrode module 200, and a second guide rail 112 is provided on the side of the positive electrode module 200 away from the negative electrode module 100. The first guide rail 111 and the second guide rail 112 extend along a first direction X. In some embodiments, grooved surfaces are formed on both sides of the side of the negative electrode module 100 away from the positive electrode module 200 as the first guide rail 111; and grooved surfaces are formed on both sides of the side of the positive electrode module 200 away from the negative electrode module 100 as the second guide rail 112.

[0060] In this embodiment, the limiting rail 302 set on the fixed side plate 301 cooperates with the first guide rail 111 and the second guide rail 112, and the multifunctional film transfer box 1 is inserted into the first mounting frame 300 along the first direction X.

[0061] The multifunctional transfer cartridge 1 of this application can realize many functions such as transfer and staining. Taking the transfer process using the multifunctional transfer cartridge as an example, the sealing performance of the transfer cartridge affects the uniform distribution of the buffer solution in the transfer cartridge during the transfer process. In order to improve the sealing performance of the multifunctional transfer cartridge and improve the transfer effect, the embodiments of this application further improve the structure of the multifunctional transfer cartridge 1. As shown in Figures 7 and 8, the negative electrode module 100 includes a first housing 110, a negative electrode plate 120 and a third housing 130, with the negative electrode plate 120 disposed between the first housing 110 and the third housing 130. In some embodiments, the first housing 110 has a first side surface 101 facing the positive electrode module 200, a first receiving groove 102 is provided on the first side surface 101, a first mesh plate 104 is provided in the first receiving groove 102, and a first groove 103 is provided on the outer periphery of the first receiving groove 102. In some embodiments, a ramp structure is formed on one side of the first groove 103. The first groove 103 is used to engage the positive electrode module 200 on one hand and to guide the positive electrode module 200 during the engagement process on the other hand.

[0062] In some embodiments, the first housing 110, the negative electrode plate 120, and the third housing 130 of this application are sealed in the following manner: the first housing 110 and the third housing 130 of the negative electrode module 100 are connected by screws; the first housing 110 is designed with snap-fit ​​mounting holes around its perimeter, and the snap-fits are fixed to the first housing 110 by pins. Further, the snap-fits installed on the negative electrode module 100 engage with the positive electrode module 200. The first housing 101 of the negative electrode module 100 is designed with two grooves, incorporating a sealing strip; the negative electrode plate 120 is then fixed to the first housing 110 by screws, pressing it against the sealing strip.

[0063] In some embodiments, a plurality of first protrusions 105 are provided on the first mesh plate 104, the first protrusions 105 are used to support the negative electrode plate 120, and a liquid storage cavity is formed between the first mesh plate 104 and the negative electrode plate 120.

[0064] In some embodiments, a negative circuit interface female connector 106 is provided on the first housing 110 to realize the circuit connection of the negative module 100.

[0065] As shown in Figures 9-11, the positive electrode module 200 includes a second housing 210, a positive electrode plate 220, and a fourth housing 240, with the positive electrode plate 220 located between the second housing 210 and the fourth housing 240. The second housing 210 and the fourth housing 240 of the positive electrode module 200 are connected by screws. The second housing 210 of the positive electrode module 200 has two grooves with embedded sealing strips. The positive electrode plate 220 is then fixed to the second housing 210 with screws to press the sealing strips together.

[0066] In some embodiments, a second mesh plate 205 is provided in the second receiving groove 202. The second mesh plate 205 is designed with a plurality of second protrusions 206. The second protrusions 206 are used to abut against the positive electrode plate 220, so that a liquid storage cavity is formed between the second mesh plate 205 and the positive electrode plate 220.

[0067] In some embodiments, the second housing 210 has a second side surface 201 facing the negative electrode module 100. A second receiving groove 202 is provided on the second side surface 201, and a second recess 203 is provided on the outer periphery of the second receiving groove 202. A first protruding edge 204 is provided on the inner side of the second recess 203. When the negative electrode module 100 and the positive electrode module 200 are engaged, the first protruding edge 204 is embedded in the first recess 103. In some specific embodiments, the second recess 203 is used to install a first sealing strip 207. The first protruding edge 204 provided on the inner side of the second recess 203 corresponds to the first recess 103 provided on the first housing 110. When the negative electrode module 100 is engaged, the negative electrode module 100 can press the first sealing strip 207, and the first protruding edge 204 can act as a guide. After the positive electrode module and the negative electrode module are engaged, a sealing strip is embedded between the two side electrode plates and the corresponding lower housing, and a sealing ring is also embedded between the housings of each module. Furthermore, a sealing ring is also installed on the liquid inlet connector on the positive electrode module 200, making the entire cavity a sealed cavity during liquid inlet and outlet processes. This application improves the sealing performance inside the multifunctional transfer chamber through improvements to the transfer chamber itself, thereby enhancing the uniformity of the liquid within the transfer chamber's containment cavity and improving the transfer performance of the transfer chamber.

[0068] As shown in Figures 11-15, a separator ring 230 is provided on the second screen plate 205, and at least a notch 231 is provided on the outer ring of the separator ring 230. The separator ring 230 of this application can solve the problem of differences in the size of the glue used in the experiment, such as mid-sized glue or mini glue. Different separator rings 230 can be embedded on the second screen plate 205 to limit the placement of the glue, reduce the ionization caused by excess liquid space, and ensure that the current does not vary too much when the glue of the same size is transferred to the film each time.

[0069] In some embodiments, the notches 231 distributed on the septum 230 are located on the upper and lower edges of the septum 230. The notches 231 are used to connect the liquid between the internal cavities of the multifunctional transfer box, so as to avoid uneven internal pressure of the liquid or local liquid accumulation, which would affect the transfer or dyeing effect.

[0070] In some embodiments, the upper and lower edges of the septum ring 230 of this application are provided with first edges 232. The thickness of the first edges is less than the thickness of the body of the septum ring 230. The thinner first edges 232 distributed on the upper and lower edges of the septum ring 230 facilitate liquid flow and prevent liquid from accumulating at the edge of the septum ring 230, which would affect the film transfer or dyeing effect.

[0071] As shown in Figures 6, 18, and 19, the transfer box and multifunctional transfer apparatus module of this application have multiple uses, such as enabling membrane transfer and staining. Furthermore, the multifunctional transfer apparatus module in this embodiment integrates multiple modules to allow for simultaneous execution of multiple experiments, enabling membrane transfer and staining to be performed simultaneously in one instrument. To prevent misassembly of the transfer box, the multifunctional transfer box of this application is equipped with an identification mechanism.

[0072] In some embodiments, the identification mechanism 500 of this application includes an identification element 501, a first identification point 502 disposed on the positive electrode module 200, and a second identification point 503 disposed on the negative electrode module 100. In some embodiments, the identification mechanism 500 includes two optocouplers as identification elements 501, and corresponding to the two optocouplers are two elastic pin mechanisms 504, used to identify the insertion of the multifunctional transfer cartridge. In some embodiments, the first identification point 502 and the second identification point 503 of the transfer cartridge for transfer are both convex structures. When the two convex points abut against the pin mechanisms 504 of the identification mechanism 500, the identification point is displaced, and the identification mechanism 500 determines whether the positive electrode module and the negative electrode module for transfer are correct.

[0073] In some embodiments, the identification point of the transfer box for dyeing is set as a concave point. When the multifunctional transfer box is misinstalled, one of the two optocouplers' pin mechanisms 504 does not displace, and the identification mechanism 500 can determine whether the transfer box is correctly inserted.

[0074] As shown in Figures 6 and 9, the second housing 210 has perforated bosses on its upper and lower sides. The threaded quick-connect fitting 107 of this application has a sealing ring inserted through this hole and locked with a nut. The positive electrode module 200 of this application has a positive electrode circuit interface female connector 208 mounted on the perforated boss of the second housing 210. Fluororubber tubing can also be fitted around the outer periphery of the circuit structure of this application to increase sealing performance and safety.

[0075] As shown in Figure 9, the multifunctional transfer box in this embodiment includes a temperature sensor 209, which can solve the problem that the temperature of the transfer box during the transfer process is not monitored in the prior art. If the temperature is too high, the adhesive may burn. The temperature sensor of this application monitors the temperature of the transfer box during the transfer process. If the temperature is too high, the adhesive may burn, and the experimental parameters can be adjusted in time.

[0076] As shown in Figures 19 and 20, in some embodiments, the multifunctional transfer box 1 further includes a liquid circulation system 800. The multifunctional transfer box of this application is inserted into the transfer apparatus along the first direction X. In actual use, the liquid inlet of the multifunctional transfer box is located at the bottom, and the liquid outlet is located at the top. This bottom-to-top liquid inlet method solves the problem of uneven liquid distribution within the multifunctional transfer box's cavity during transfer or dyeing processes in the prior art. A handle is provided on one side of the positive electrode module 200, and two quick-connect liquid lines 107 are provided on the opposite side of the handle. The two quick-connect liquid lines 107 are located on the same side of the multifunctional transfer box 1, facilitating the design of the liquid system and structure of this application and simplifying the liquid inlet and outlet of the multifunctional transfer box 1.

[0077] In some embodiments, the liquid circulation system 800 of this application is disposed on the outer periphery of the second receiving tank 202, and the main body of the liquid circulation system 800 is mounted on the second housing 210. The liquid circulation system 800 also includes a first inlet 803, which is disposed at the lower end of the liquid circulation system 800. A quick-connect fitting 107 for liquid inlet delivers buffer solution to the first inlet 803 through a pipe communicating with the first inlet 803, and the buffer solution is delivered from the first inlet 803 to the receiving cavity. In some embodiments, this application provides two inlets below the second housing 210 for delivering buffer solution. In some embodiments, the liquid circulation system 800 also includes a second inlet 805. The second housing 210 has a first central axis O1, which extends along a third direction Z. The first inlet 803 and the second inlet 805 are located on both sides of the first central axis O1, and the first inlet 803 and the second inlet 805 are distributed along a first direction X. In some embodiments, the first inlet 803 and the second inlet 805 are located at the same height. This application introduces buffer solution through two inlets located below the second housing 210, which not only allows the buffer solution to be delivered from below to the multifunctional transfer cartridge, but also simplifies the inlet settings of the buffer solution. That is, the buffer solution is uniformly delivered by inlets located on both sides of the first central axis O1.

[0078] In some embodiments, the quick-connect fitting 107 for liquid inlet in this application is connected to the first inlet 803 via a first pipe 808 and a second pipe 809. The first pipe 808 is connected to the second pipe 809 via a tee fitting. The two sections of the second pipe 809 are connected to the first inlet 803 and the second inlet 805, respectively.

[0079] The liquid circulation system 200 in this embodiment further includes a first outlet 804, which is connected to a quick-connect liquid line connector 107 for liquid discharge. The first outlet 804 is located at the upper end of the first housing 110. Excess buffer solution is sent from the first outlet 804 to the quick-connect liquid line connector 107 for liquid discharge, thereby realizing the discharge of waste liquid from the multifunctional transfer film box.

[0080] In some embodiments, the liquid circulation system of this application further includes a second outlet 806 and a third outlet 807. The first outlet 804, the second outlet 806 and the third outlet 807 are distributed at intervals along the first direction X. For example, in some specific embodiments, the first outlet 804, the second outlet 806 and the third outlet 807 of this application are located at the same height, and the first outlet 804 and the third outlet 807 are located on both sides of the first central axis O1, and the second outlet 806 is located close to the first central axis O1. This application provides three outlets to facilitate better discharge of waste liquid from the multifunctional transfer film box.

[0081] In some embodiments, as shown in Figures 3 and 4, the first outlet 804, the second outlet 806, and the third outlet 807 are connected by a third conduit 813. In other embodiments, the quick-connect fitting 107 for liquid discharge is connected to the three outlets respectively via the third conduit 813. In still other embodiments, a buffer tube 814 is provided between the first outlet 804 and the second outlet 806, providing more buffer space for the waste liquid, thereby improving the stability of the discharged liquid and enhancing the transfer or dyeing effect.

[0082] In some embodiments, two buffer pipes 814 are provided between the first outlet 804 and the second outlet 806, and the buffer pipes 814 are connected to the third pipe 813 through a tee.

[0083] In some embodiments, both the inlet and outlet of this application are connected to a pipeline via connectors 810. In some specific embodiments, the first inlet 803, the second inlet 805, the first outlet 804, the second outlet 806, and the third outlet 807 of this application are all equipped with connectors 810. The connectors 810 of this application have right-angle mounting tubes 811, which are disposed on the mounting base 812. One end of the right-angle mounting tube 811 passes through the mounting base 812 and communicates with the receiving cavity of the multifunctional transfer film box. The right-angle mounting tubes 811 at the inlet and outlet of this application prevent blockage during the solution flow process and can improve the stability of liquid inlet and outlet.

[0084] In some embodiments, the mounting submodule 3 includes a fixing base plate 303, on which a fixing mechanism 400 is provided. The fixing mechanism 400 includes a connector assembly 450, and the liquid quick-connect connector on the multi-functional transfer box 1 is connected to the connector assembly 450.

[0085] As shown in Figures 21, 22, and 23, this embodiment of the application provides a fixing mechanism 400 for a multifunctional transfer cartridge connector. The fixing mechanism 400 connects the quick-connect liquid connector 107 to the connector assembly 450. The connector assembly 450 is connected via a connecting pipe to enable the inlet and outlet of the buffer solution in the multifunctional transfer cartridge 1. The fixing mechanism 400 in this embodiment includes a first fixing mechanism 410, which includes a first fixing base 411. The first fixing base 411 has a first through hole 412 for the transfer cartridge liquid connector to pass through. In some embodiments, the first fixing base 411 protrudes in a direction away from the second fixing base 420 to form a first boss 413. The first boss 413 and the second fixing base 420 form a first cavity 430, which is used to accommodate one end of the connector assembly 450.

[0086] In some embodiments, fixing ears 414 are provided on both sides of the first boss 413, and a fourth through hole 105 is provided on the fixing ear 104 for the first fixing member 416 to pass through. The two fourth through holes 415 are located on both sides of the first boss 413, and the first fixing member 416 passes through the fourth through hole 415 to fix the first fixing base 411 and the second fixing base 420.

[0087] The second fixing mechanism 420 in this embodiment includes a second fixing base 420. In some embodiments, the second fixing base 420 is a strip-shaped structure. The first fixing base 411 is fixed on the second fixing base 420 by a first fixing member 416. The second fixing base 420 is provided with a second through hole 422 for the connector assembly 450 to pass through.

[0088] In some embodiments, the second fixing base 420 is provided with a third through hole 423 for the second fixing member 424 to pass through. In some embodiments, the third through hole 423 is located on both sides of the second fixing base 420, and the second through hole 422 is located between the two third through holes 423. The second fixing base 420 of this application is mounted on the fixing substrate 303. In some embodiments, the first fixing member 416 and the second fixing member 424 in this application can be selected as screws or screw structures with end caps.

[0089] In some embodiments, the diameter of the second fixing member 424 is smaller than the diameter of the third through hole 423. The second fixing member 424 achieves axial fixation of the second fixing base 420. Since the diameter of the second fixing member 424 is smaller than the diameter of the third through hole 423, the second fixing base 420 can be adjusted in position around the second fixing member 424 with a slight range.

[0090] In this embodiment, the fixed base plate 303 is a mounting plate structure. An adjusting element 440 is provided between the second fixed base 420 and the fixed base plate 303. Both ends of the adjusting element 440 abut against the fixed base plate 303 and the second fixed base 420, respectively. In some specific embodiments, the adjusting element 440 is a spring structure. A first groove 3031 is provided on the fixed base plate 3031. One end of the adjusting element 440 abuts against the bottom surface of the first groove 3031, and the other end abuts against the second fixed base 420. The adjusting element 440 can further fix the second fixing mechanism 420, preventing the second fixing mechanism 420 from shaking due to the difference in aperture between the third through hole 423 and the second fixing member 424, and does not affect the adjustment of the position of the second fixing mechanism. In some specific embodiments, the adjusting element 440 is located between the second fixing member 424 and the first boss 413, with both ends abutting against the second fixed base 420 and the fixed base plate 303, respectively.

[0091] The connector 450 of this application includes a first connecting seat 451 and a second connecting seat 452. The diameter of the first connecting seat 451 is smaller than the diameter of the second through hole 422, and the diameter of the second connecting seat 452 is larger than the diameter of the second through hole 422. The first connecting seat 302 passes through the second through hole 422, and the second fixing base 420 is locked between the first connecting seat 451 and the second connecting seat 452, thus fixing the connector assembly 450 and the second fixing base 420. The first connecting seat 451 of the connector 3 is located between the first cavity 110 formed by the first boss 301. The first fixing base 411 and the second fixing base 102 together fix the connector assembly 450. The fine adjustment of the position of the second fixing base 420 ensures that the installation position of the connector assembly 450 can be finely adjusted. As shown in Figure 2, the connector assembly 450 of this application has a connecting hole 453 on one side of the first connecting seat 451. The connecting hole 453 is used to connect with the liquid quick connector on the transfer box.

[0092] In some embodiments, the diameter of the first through hole 412 of this application is larger than the diameter of the liquid connector, and the diameter of the first through hole 412 gradually decreases along the thickness direction of the first fixed base 411. The inner wall of the first through hole 412 forms a frustum structure, which forms a guide mechanism. When the liquid connector of the transfer film box of this application passes through the first through hole 412 and is inserted into the connection hole 453, since the diameter of the first through hole 412 is larger than the diameter of the liquid connector, the situation where the liquid connector cannot be inserted into the first through hole 412 due to tolerances in the processing is avoided. The inner wall of the first through hole 412 forms a guide mechanism, which facilitates the insertion of the liquid connector. The connector assembly 450 connected to the liquid connector can be finely adjusted in position. The adjusting element 440 has a floating support function for the second fixed base 420. The first through hole 412 on the first fixed base 411 has a certain guiding angle, which ensures that the liquid connector and the connector assembly 450 can be smoothly connected when the transfer film box is inserted.

[0093] As shown in Figure 23, the liquid circulation system 800 of this application also includes a solenoid valve 820 and a pump body 830. In the liquid circulation system 800 of this application, the solenoid valve 820 controls the inflow of buffer solution and the outflow of waste liquid, eliminating the need for a waste liquid tank for collection, making operation more convenient and faster. The multifunctional transfer apparatus of this application can achieve high-throughput transfer or staining, improving work efficiency. Furthermore, the multifunctional transfer apparatus of this application can also be equipped with indicator lights to indicate the working status. Electrical equipment and connection methods not mentioned in this application can be implemented using existing technologies.

[0094] As shown in Figure 24, the multifunctional transfer apparatus of this application was used for transfer experiments. The experimental conditions were as follows: a PVDF membrane with a thickness of 10 mm was used; the buffer filling rate was 2.5 mL / min; the voltage was controlled at 25 V; the transfer time was 15 min; and 1.5X buffer was used. The current / voltage trend was monitored during the transfer process. The transfer effect is shown in Figure 24. The transferred strips of this application were uniform, and the pressure fluctuations during the transfer process were controlled within the range of 3 kPa to 7 kPa.

Claims

1. A multi-functional film transfer machine characterized by comprising: The multifunctional transfer apparatus includes a multifunctional transfer box (1) and an installation submodule (3) for mounting the multifunctional transfer box (1); the multifunctional transfer apparatus has a first direction (X), a second direction (Y) and a third direction (Z), and the first direction (X), the second direction (Y) and the third direction (Z) intersect each other; The multifunctional transfer film box (1) includes a negative electrode module (100) and a positive electrode module (200). A first guide rail (111) is provided on the side of the negative electrode module (100) away from the positive electrode module (200), and a second guide rail (112) is provided on the side of the positive electrode module (200) away from the negative electrode module (100). The first guide rail (111) and the second guide rail (112) extend along the first direction (X). The installation submodule (3) includes a first installation frame (300), the first installation frame (300) includes a fixed side plate (301) extending along a third direction (Z), the fixed side plate (301) is located on both sides of the multifunctional transfer box (1), and the inner side wall of the fixed side plate (301) is provided with a limiting track (302), the limiting track (302) extends along a first direction (X), the limiting track (302) cooperates with the first guide track (111) and the second guide track (112), and the multifunctional transfer box (1) is inserted into the first installation frame (300) along the first direction (X).

2. The multi-functional film converter according to claim 1, wherein The negative electrode module (100) forms grooved surfaces on both sides of the side away from the positive electrode module (200) as the first guide rail (111); the positive electrode module (200) forms grooved surfaces on both sides of the side away from the negative electrode module (100) as the second guide rail (112).

3. The multi-functional film converter according to claim 2, wherein The fixed side plate (301) forms a limiting track (302) on the inner side wall, which is spaced along the third direction (Z). The negative electrode module (100) or the positive electrode module (200) is installed on the first mounting frame (300) through the limiting track (302).

4. The multi-functional film converter according to claim 1, wherein The positive electrode module (200) of the multifunctional transfer film box (1) includes a second housing (210), and two liquid quick-connect connectors (107) are provided on the side wall of the second housing (200); a liquid circulation system (800) is provided on the side of the second housing (210) away from the negative electrode module (100), and the liquid circulation system (800) includes a first inlet (803) connected to the liquid quick-connect connector (107) for liquid inlet and a first outlet (804) connected to the liquid quick-connect connector (107) for liquid outlet; the first inlet (803) is located at the lower end of the second housing (210), and the first outlet (804) is located at the upper end of the second housing (210).

5. The multi-functional film converter according to claim 1, wherein The first inlet (803) and the first outlet (804) are equipped with a connector (810), the connector (810) has a right-angle mounting tube (811), the right-angle mounting tube (811) is disposed on the mounting base (812), and one end of the right-angle mounting tube (811) penetrates the second housing (210).

6. The multi-functional film converter according to claim 4, wherein The liquid circulation system (800) includes several outlets, with a buffer tube (814) connected between at least two outlets.

7. The multi-functional film converter according to claim 1, wherein The installation submodule (3) includes a fixed base plate (303) extending along a third direction (Z), and a fixing mechanism (400) is provided on the fixed base plate (303). The fixing mechanism (400) includes a connector assembly (450), and the liquid quick-connect connector (107) on the multi-functional transfer box (1) is connected to the connector assembly (450).

8. The multi-functional film converter according to claim 7, wherein The fixing mechanism (400) includes: The first fixing mechanism (410) includes a first fixing base (411) and a first through hole (412) for the connector to pass through. A second fixing mechanism (420) includes a second fixing base (421) having a second through hole (422) for the connector assembly (450) to pass through. A first fixing base (411) is fixed to the second fixing base (421). A third through hole (423) is provided on the second fixing base (411) for a second fixing member (424) to pass through. The diameter of the second fixing member (424) is smaller than the diameter of the third through hole (423). And / or, The second fixing base (421) is fixed to the fixing base plate (303) by the second fixing member (424), and an adjusting element (440) is provided between the second fixing base (421) and the fixing base plate (303), with both ends of the adjusting element (440) abutting against the fixing base plate (303) and the second fixing base (421) respectively; and / or, The first fixing base (411) protrudes in a direction away from the second fixing base (421) to form a first boss (413), and the first boss (413) and the second fixing base (421) form a first cavity (430), the first cavity (430) being used to accommodate one end of the connector assembly (450); and / or, Along the thickness direction of the first fixed base (411), the diameter of the first through hole (412) gradually decreases.

9. The multi-functional film converter according to claim 7, wherein The connector assembly (450) includes a first connector (451) and a second connector (452), wherein the diameter of the first connector (451) is smaller than the diameter of the second through hole (422), and the diameter of the second connector (452) is larger than the diameter of the second through hole (422).

10. The multi-functional film converter according to claim 1, wherein Further comprising an identification mechanism (500), the identification mechanism (500) comprises an identification element (501), a first identification site (502) and a second identification site (503); the first identification site (502) is located on the positive electrode module (200), and the identification element (501) is used to identify whether the positive electrode module (200) and the negative electrode module (100) are in place and whether the module is wrong.

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