Minicircle DNA platform for r-loop production and visualization
The minicircle DNA platform addresses the limitations of existing R-loop formation models by using recombinase-mediated excision to create small, supercoiled scaffolds for high-resolution imaging and screening, enhancing our understanding of R-loop biology and therapeutic development.
WO2026112638A1PCT designated stage Publication Date: 2026-05-28EMORY UNIVERSITY
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Patent Information
- Application Number
- PCT/US2025/057012
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-11-25
- Filing Date
- 2025-11-25
- Publication Date
- 2026-05-28
AI Technical Summary
Technical Problem
Current in vitro models for R-loop formation use large bacterial plasmids that are structurally heterogeneous and topologically complex, making them unsuitable for high-resolution imaging and limiting the understanding of functional versus pathological R-loops, which hinders mechanistic understanding and drug discovery efforts.
Method used
A minicircle DNA (mcDNA) platform is developed using recombinase-mediated excision to produce small, supercoiled minicircle DNA scaffolds for R-loop formation, enabling high-resolution visualization and screening for R-loop-binding agents.
Benefits of technology
Enables stable R-loop formation compatible with cryo-EM and AFM analysis, facilitating the study of genome regulation and development of R-loop-modulating therapeutics.
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Smart Images
Figure US2025057012_28052026_PF_FP_ABST
Abstract
The present disclosure relates to methods for producing minicircle DNA (mcDNA) and methods of using the mcDNA as a scaffold to produce R-loops. Further disclosed are methods of screening for an agent that binds an R-loop.
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