Method for analyzing absorption of sample into skin by using raman spectroscopy

Abstract

The present invention relates to a method for analyzing absorption of a sample into the skin by using Raman spectroscopy, wherein absorption of a target component contained in the sample into the skin is intuitively analyzed through a Raman spectrum graph obtained by a Raman spectrometer. According to an embodiment of the present invention, the absorption of the target component into the skin at different skin depths can be measured without damaging the skin by using Raman spectroscopy, and the absorption of the target component into the skin and the absorption pattern thereof can be intuitively identified by outputting, as a three-dimensional graph, data obtained by the Raman spectrometer, thereby enabling efficient analysis of characteristics of the target component contained in the sample.

Description

Method for analyzing skin absorption of a sample using Raman spectroscopy

[0001] The present invention relates to a method for analyzing the amount of skin absorption of a sample using Raman spectroscopy, which intuitively analyzes the amount of skin absorption of a target component contained in a sample through a Raman spectrum graph obtained via a Raman spectrometer.

[0002] The present application claims priority based on Korean Patent Application No. 10-2025-0020824 filed on February 18, 2025, and all contents described in the specification and drawings of said application are incorporated by reference into the present application.

[0003] The skin consists of a three-layer structure comprising the epidermis, dermis, and subcutaneous fat layer. Among these, the stratum corneum is located in the outermost layer of the epidermis and serves to protect the body from external stimuli. The stratum corneum is composed of multiple layers of keratinized cells, and a hydrophobic protein called keratin fills these cells. Additionally, the spaces between the keratinized cells are filled with intercellular lipids composed of ceramides, free fatty acids, cholesterol, etc., forming a lamellar structure.

[0004] While these structural characteristics of the epidermis inhibit moisture evaporation and protect the human body, they also hinder the easy absorption of drugs or active ingredients. Consequently, various transdermal formulations are being developed to overcome this limitation, and technologies related to methods for quantitatively measuring the transdermal absorption effects of these formulations are also required.

[0005] Skin absorption testing methods for cosmetics differ from the evaluation of skin permeability of pharmaceuticals intended to reach the systemic circulation system, and since the site of action of functional active ingredients is skin cells, there is a need to develop technology to evaluate how much of the active ingredient has been absorbed into the skin layer.

[0006] Meanwhile, molecules possess unique characteristics, and Raman spectroscopy is a highly sensitive spectroscopic analysis technique that can confirm the presence of specific components by analyzing the intrinsic vibrations of molecules, and it is being utilized in various fields.

[0007] In particular, Raman spectroscopy is widely used in skin and cosmetics research to quantitatively measure and analyze skin changes before and after product application; however, since Raman spectroscopy displays the amount of ingredients absorbed by the skin only as a linear graph, it has the disadvantage that results cannot be intuitively verified.

[0008] Therefore, there is a need for technology that can effectively compare and analyze pre- and post-measurement data by intuitively representing the patterns and characteristics of data acquired through Raman spectroscopy.

[0009] The technical problem that the present invention aims to solve is to provide a method for analyzing the skin absorption amount of a sample using Raman spectroscopy, which can measure the absorption amount of a target component according to skin depth without damaging the skin, and can intuitively understand the skin absorption amount and absorption pattern of the target component by outputting the data obtained through the Raman spectrometer as a three-dimensional graph, thereby enabling efficient analysis of the characteristics of the target component in the sample.

[0010] The objectives of the present invention are not limited thereto, and other unmentioned objectives will be clearly understood by a person skilled in the art from the description below.

[0011] An embodiment of the present invention for achieving the above technical problem comprises, to achieve this objective, a method for analyzing the amount of skin absorption of a target component contained in a sample through a Raman spectrum graph obtained via a Raman spectrometer, comprising: (a) a step of obtaining skin Raman data (SRD) containing multiple Raman spectra (1SP) by measuring multiple times from a measurement area on the skin surface to a preset measurement depth using the Raman spectrometer; (b) a step of applying the sample to the measurement area, and then measuring multiple times from the measurement area to the measurement depth using the Raman spectrometer to obtain sample application data (RRD) containing multiple Raman spectra (2SP); (c) a step of calculating a representative value (1A) for each of a plurality of Raman spectra (1SP) of the skin Raman data (SRD) to generate a first representative value data (1AD) including a plurality of representative values ​​(1A), and a step of calculating a representative value (2A) for each of a plurality of Raman spectra (2SP) of the sample application data (RRD) to generate a second representative value data (2AD) including a plurality of representative values ​​(2A); and (d) a step of generating a three-dimensional graph (3DG-1) before sample application using the first representative value data (1AD), and generating a three-dimensional graph (3DG-2) after sample application using the second representative value data (2AD); the present invention provides a method for analyzing the amount of skin absorption of a sample using Raman spectroscopy, characterized by including:

[0012] According to an embodiment of the present invention, the amount of absorption of a target component according to skin depth can be measured without skin damage using Raman spectroscopy, and the amount of absorption and absorption pattern of the target component in the skin can be intuitively understood by outputting the data obtained through the Raman spectrometer as a three-dimensional graph, thereby having the effect of efficiently analyzing the characteristics of the target component in the sample.

[0013] In addition, by detecting structural signs at a stage prior to when wrinkles become clearly visible to the naked eye, it is effective in identifying whether premature wrinkles are forming and providing a scientific basis for preventing or mitigating skin aging.

[0014] FIG. 1 is a flowchart of a method for analyzing the amount of skin absorption of a sample using Raman spectroscopy according to an embodiment of the present invention.

[0015] Figure 2 is a block diagram showing the configuration for performing the method of analyzing the amount of skin absorption of a sample using the Raman spectroscopy of Figure 1.

[0016] Figure 3 is a diagram showing an example of a measurement area on the skin surface.

[0017] Figure 4 is a diagram illustrating the process of setting the analysis interval of the Raman spectrum graph in step (f) of Figure 1.

[0018] FIGS. 5 to 7 are diagrams illustrating the process of generating first representative value data and second representative value data through skin Raman data and sample application data obtained through a Raman spectrometer.

[0019] Figure 8 is a diagram showing examples of a 3D graph before sample application and a 3D graph after sample application generated through the first representative value data and the second representative value data of Figure 7.

[0020] [Explanation of the symbol]

[0021] 10: Method for analyzing skin absorption of a sample using Raman spectroscopy

[0022] 100 : Raman spectrometer

[0023] 200 : Operation unit

[0024] 300 : 3D data generation unit

[0025] 400 : Measurement area

[0026] 400a: First measurement area

[0027] 400b : Second measurement area

[0028] 400c: Third measurement zone

[0029] 400d: 4th measurement area

[0030] 401 : Measurement depth

[0031] 403 : Unit interval

[0032] SP: Raman spectrum of the skin surface

[0033] RP: Raman spectrum of the target component

[0034] B: Analysis interval

[0035] SRD: Skin Raman Data

[0036] 1SRD: Skin 1 Raman Data

[0037] 2SRD: Second skin Raman data

[0038] 3SRD: Third Skin Raman Data

[0039] 4SRD: Fourth Skin Raman Data

[0040] 1SP: Multiple Raman spectra

[0041] 1A : Representative value

[0042] 1AD: First representative value data

[0043] 1AD-1: Region 1 skin representative value data

[0044] 1AD-2: Region 2 skin representative value data

[0045] 1AD-3: Region 3 skin representative value data

[0046] 1AD-4: Region 4 skin representative value data

[0047] RRD: Sample coating data

[0048] 1RRD: 1st sample application data

[0049] 2RRD: 2nd sample application data

[0050] 3RRD: Third sample application data

[0051] 4RRD: 4th Sample Coating Data

[0052] 2SP: Multiple Raman spectra

[0053] 2A : Representative value

[0054] 2AD: Second Representative Value Data

[0055] 2AD-1: Region 1 Sample Representative Value Data

[0056] 2AD-2: Representative sample data for Zone 2

[0057] 2AD-3: Representative sample data for the third region

[0058] 2AD-4: Representative sample data for Zone 4

[0059] 3DG-1: 3D graph before sample application

[0060] 3DG-2: 3D graph after sample application

[0061] Hereinafter, some embodiments of the present invention will be described in detail with reference to the exemplary drawings. It should be noted that in assigning reference numerals to the components of each drawing, the same components are given the same reference numeral whenever possible, even if they are shown in different drawings. Furthermore, in describing the present invention, if it is determined that a detailed description of related known components or functions could obscure the essence of the invention, such detailed description is omitted.

[0062] In addition, terms such as first, second, A, B, (a), (b), etc., may be used when describing the components of the present invention. These terms are intended only to distinguish the components from other components, and the essence, order, or sequence of the components is not limited by the terms. Where it is stated that a component is "connected," "combined," or "connected" to another component, it should be understood that the component may be directly connected or connected to the other component, but that another component may also be "connected," "combined," or "connected" between each component.

[0063] FIG. 1 is a flowchart of a method for analyzing the amount of skin absorption of a sample using Raman spectroscopy according to an embodiment of the present invention. FIG. 2 is a block diagram showing the configuration for performing the method for analyzing the amount of skin absorption of a sample using Raman spectroscopy of FIG. 1. FIG. 3 is a diagram showing an example of a measurement area on the skin surface. FIG. 4 is a diagram explaining the process of setting the analysis section of the Raman spectrum graph in step (f) of FIG. 1. FIG. 5 to 7 are diagrams showing the process of generating first representative value data and second representative value data through skin Raman data and sample application data obtained through a Raman spectrometer. FIG. 8 is a diagram showing examples of a 3D graph before sample application and a 3D graph after sample application generated through the first representative value data and the second representative value data of FIG. 7.

[0064] Here, FIG. 5 schematically illustrates an example of multiple Raman spectra measured at different depths from the surface of the skin, and FIG. 6 schematically illustrates an example of multiple Raman spectra measured at different depths from the surface of the skin after application of a sample.

[0065] As illustrated in the drawings, a method (10) for analyzing the amount of skin absorption of a sample using Raman spectroscopy according to an embodiment of the present invention is a method for analyzing the amount of skin absorption of a target component contained in a sample through a Raman spectrum graph obtained through a Raman spectrometer (100), comprising: (a) a step of obtaining skin Raman data (SRD) containing multiple Raman spectra (1SP) by measuring multiple times from a measurement area (400) on the skin surface to a preset measurement depth (401) through a Raman spectrometer (100); (b) a step of obtaining sample application data (RRD) containing multiple Raman spectra (2SP) by applying a sample to the measurement area (400) and then measuring multiple times from the measurement area (400) to the measurement depth (401) through a Raman spectrometer (100). (c) a step of generating a first representative value data (1AD) containing the multiple representative values ​​(1A) by calculating the representative values ​​(1A) of each of the multiple Raman spectra (1SP) of the skin Raman data (SRD), and generating a second representative value data (2AD) containing the multiple representative values ​​(2A) by calculating the representative values ​​(2A) of each of the multiple Raman spectra (2SP) of the sample application data (RRD); and (d) a step of generating a three-dimensional graph (3DG-1) before sample application using the first representative value data (1AD), and generating a three-dimensional graph (3DG-2) after sample application using the second representative value data (2AD);

[0066] In addition, the method (10) for analyzing the amount of skin absorption of a sample using Raman spectroscopy according to one embodiment of the present invention further includes: (f) a step of setting an analysis section (B) of the Raman spectrum graph prior to step (a).

[0067] The present invention is a method for analyzing the amount of skin absorption of a target component contained in a sample through a Raman spectrum graph obtained using a Raman spectrometer, wherein the sample is a product applied to the skin and the target component may be a specific component contained in the product that is absorbed into the skin.

[0068] For example, the sample cosmetic may be a target ingredient that is contained in the cosmetic and absorbed into the skin.

[0069] Hereinafter, an embodiment of the present invention will be described in detail step by step.

[0070] First, step (f) is a step of setting the analysis section (B) of the Raman spectrum graph obtained through the Raman spectrometer (100).

[0071] That is, step (f) is a step of setting the analysis section (B) of the X-axis (Raman shift) of the Raman spectrum graph obtained through the Raman spectrometer (100).

[0072] These (f) steps include: (f-1) obtaining a Raman spectrum (SP) of the skin surface through a Raman spectrometer (100); (f-2) obtaining a Raman spectrum (RP) of the target component through a Raman spectrometer (100); and (f-3) comparing the Raman spectrum (SP) of the skin surface and the Raman spectrum (RP) of the target component, and then setting one of the non-overlapping peak sections as an analysis section (B).

[0073] Step (f-1) is a step of obtaining the Raman spectrum (SP) of the skin surface by measuring the skin surface through a Raman spectrometer (100). (See FIG. 4(a))

[0074] And, step (f-2) is the step of obtaining the Raman spectrum (RP) of the target component contained in the sample through the Raman spectrometer (100). (See FIG. 4(b))

[0075] That is, step (f-2) may be a step of obtaining the Raman spectrum (RP) of retinol contained in the sample.

[0076] Next, step (f-3) is a step of comparing the Raman spectrum (SP) of the skin surface with the Raman spectrum (RP) of the target component and then setting the analysis section (B) of the X-axis (Raman shift) of the Raman spectrum graph.

[0077] Here, step (f-3) can set one of the peak sections of the target component Raman spectrum (RP) that does not overlap with the Raman spectrum (SP) of the skin surface as the analysis section (B).

[0078] That is, the analysis section (B) may be any one of the peak sections of multiple target component Raman spectra (RP) that do not overlap with the Raman spectrum (SP) of the skin surface, and which has the greatest difference from the Raman spectrum (SP) of the skin surface.

[0079] This analysis interval (B) is the peak interval of the Raman spectrum (RP) of the target component, from 1560 to 1620 cm. -1 It may be. (See Fig. 4(c))

[0080] In this way, by setting the peak section of the target component's Raman spectrum (RP) that does not overlap with the skin surface's Raman spectrum (SP) as the analysis section (B), the amount of skin absorption of the target component can be analyzed more efficiently by minimizing the overlap with the skin's inherent Raman spectrum even when the molecular weight of the target component is low.

[0081] Furthermore, step (a) according to one embodiment of the present invention is to obtain skin Raman data (SRD) by measuring a measurement area (400) on the skin surface through a Raman spectrometer (100).

[0082] More specifically, step (a) involves obtaining skin Raman data (SRD) by taking multiple measurements from the measurement area (400) to the measurement depth (401) along a preset unit interval (403).

[0083] That is, step (a) is a step in which a Raman spectrometer (100) obtains multiple Raman spectra (1SP) by measuring multiple times from a measurement area (400) on the skin surface along a unit interval (403) to a measurement depth (401) of the skin, and generates skin Raman data (SRD) through the multiple Raman spectra (1SP).

[0084] That is, the skin Raman data (SRD) obtained in step (a) may include each Raman spectrum (1SP) measured at a plurality of points spaced apart along a unit interval (403) from the skin surface.

[0085] Here, the plurality of Raman spectra (1SP) are linear graphs, the preset unit interval (403) may be 5 μm, and the preset measurement depth (401) may be 50 μm (see FIG. 3 and FIG. 4).

[0086] Meanwhile, step (a) can divide the measurement area (400) into multiple measurement areas (400a, 400b, 400c, 400d) and then generate skin Raman data (1SRD, 2SRD, 3SRD, 4SRD) for each divided measurement area (400a, 400b, 400c, 400d).

[0087] To explain the step (a) more specifically, the step (a) includes: (a-1) dividing the measurement area (400) into multiple measurement areas (400a, 400b, 400c, 400d); and (a-2) generating multiple skin Raman data (1SRD, 2SRD, 3SRD, 4SRD) by measuring each of the multiple measurement areas (400a, 400b, 400c, 400d) multiple times up to the measurement depth (401) using a Raman spectrometer.

[0088] (a-1) Step is to divide the measurement area (400) on the skin surface in the width direction of the skin.

[0089] (a-1) Step, for example, can divide the measurement area (400) into four parts: a first measurement area (400a), a second measurement area (400b), a third measurement area (400c), and a fourth measurement area (400d).

[0090] Step (a-2) is a step of generating skin Raman data (1SRD, 2SRD, 3SRD, 4SRD) for each of the multiple measurement areas (400a, 400b, 400c, 400d) divided in Step (a-1).

[0091] More specifically, step (a-2) is a step of measuring each divided measurement area (400a, 400b, 400c, 400d) multiple times along the unit interval (403) to the measurement depth (401), and then generating skin Raman data (1SRD, 2SRD, 3SRD, 4SRD) for each divided measurement area (400).

[0092] For example, step (a-2) can generate first skin Raman data (1SRD), second skin Raman data (2SRD), third skin Raman data (3SRD), and fourth skin Raman data (4SRD) by having the Raman spectrometer (100) measure each of the first measurement area (400a) to the fourth measurement area (400d) multiple times along the unit interval (403) to the measurement depth (401).

[0093] Here, the first skin Raman data (1SRD) to the fourth skin Raman data (4SRD) may each include a plurality of Raman spectra (1SP).

[0094] Continuing, step (b) is a step of applying a sample to a measurement area (400) and allowing it to absorb for a certain period of time, and then measuring the measurement area (400) where the sample is applied using a Raman spectrometer (100).

[0095] That is, step (b) is a step in which a Raman spectrometer (100) measures a measurement area (400) coated with a sample to obtain sample coating data (RRD).

[0096] (b) In the same way as in (a), the Raman spectrometer (100) obtains multiple Raman spectra (2SP) by measuring multiple times from the measurement area (400) to the measurement depth (401) of the skin along the unit interval (403), and generates sample application data (RRD) through the multiple Raman spectra (2SP).

[0097] Meanwhile, step (b) can generate sample coating data (1RRD, 2RRD, 3RRD, 4RRD) for each divided measurement area (400a, 400b, 400c, 400d), just like step (a).

[0098] That is, step (b) generates multiple sample coating data (1RRD, 2RRD, 3RRD, 4RRD) by measuring each of the multiple measurement regions (400a, 400b, 400c, 400d) multiple times along the unit interval (403) to the measurement depth (401) through the Raman spectrometer (100).

[0099] For example, step (b) may generate first sample coating data (1RRD), second sample coating data (2RRD), third sample coating data (3RRD), and fourth sample coating data (4RRD) by having the Raman spectrometer (100) measure each of the first measurement area (400a) to the fourth measurement area (400d) multiple times along the unit interval (403) to the measurement depth (401).

[0100] Here, the first sample coating data (1RRD) to the fourth sample coating data (4RRD) may each include a plurality of Raman spectra (2SP).

[0101] As such, one embodiment of the present invention divides the measurement area (400) into a plurality of measurement areas (400a, 400b, 400c, 400d) and generates a plurality of skin Raman data (1SRD, 2SRD, 3SRD, 4SRD) for each measurement area (400a, 400b, 400c, 400d), thereby allowing the absorption amount and absorption pattern of the target component for the measurement area (400) to be shown in more detail compared to when the measurement area (400) is measured overall through a Raman spectrometer.

[0102] Continuing, step (c) is a step in which the computation unit (200) receives skin Raman data (SRD) and sample application data (RRD) generated from the Raman spectrometer (100) and generates first representative value data (1AD) and second representative value data (2AD).

[0103] That is, in step (c), the operation unit (200) calculates a representative value (1A) for each of the multiple Raman spectra (1SP) of the skin Raman data (SRD) and generates a first representative value data (1AD) containing the multiple representative values ​​(1A).

[0104] Additionally, in step (c), the operation unit (200) calculates a representative value (2A) for each of the multiple Raman spectra (2SP) of the sample coating data (RRD) and generates a second representative value data (2AD) containing the multiple representative values ​​(2A).

[0105] To explain step (c) more specifically, step (c) includes: (c-1) a step of generating first representative value data (1AD) by setting each area value formed by a plurality of Raman spectra (1SP) of skin Raman data (SRD) in the analysis section (B) as a plurality of representative values ​​(1A); and (c-2) a step of generating second representative value data (2AD) by setting each area value formed by a plurality of Raman spectra (2SP) of sample application data (RRD) in the analysis section (B) as a plurality of representative values ​​(2A).

[0106] First, step (c-1) is a step in which the calculation unit (200) calculates the area value formed by each Raman spectrum (1SP) measured along the unit interval (403) in the analysis section (B), and then sets each area value as the representative value (1A) of each Raman spectrum (1SP).

[0107] (c-1) In step (c-1), the calculation unit (200) can generate the first area skin representative value data (1AD-1) by calculating the area value of each Raman spectrum (1SP) of the first skin Raman data (1SRD).

[0108] Of course, in step (c-1), the operation unit (200) can calculate the area value of the Raman spectrum (1SP) of each of the second skin Raman data (2SRD), third skin Raman data (3SRD), and fourth skin Raman data (4SRD) to generate the second area skin representative value data (1AD-2), third area skin representative value data (1AD-3), and fourth area skin representative value data (1AD-4).

[0109] Here, the area value is the region formed by one Raman spectrum (1SP) in the analysis section (B), and may be the region under the graph (curve) of one Raman spectrum (1SP) in the analysis section (B).

[0110] That is, the area value formed by each Raman spectrum (1SP) is a quantitative analysis indicator for each Raman spectrum (1SP), and can be obtained by integrating the region formed by each Raman spectrum (1SP) in the analysis section (B).

[0111] For example, the area value (1A-1) for the Raman spectrum (1SP-1) of the skin surface in the measurement area (400) may be '2.26829'.

[0112] And, step (c-2) is a step in which the calculation unit (200) calculates the area value formed by each Raman spectrum (2SP) measured along the unit interval (403) in the analysis section (B), and sets each area value as the representative value (2A) of each Raman spectrum (2SP).

[0113] (c-2) In step (c-2), the calculation unit (200) can generate the first region sample representative value data (2AD-1) by calculating the area value of each Raman spectrum (2SP) of the first sample coating data (1RRD).

[0114] Of course, in step (c-2), the operation unit (200) can calculate the area values ​​of the Raman spectra (2SP) of the second sample coating data (2RRD), the third sample coating data (3RRD), and the fourth sample coating data (4RRD), and generate the second region sample representative value data (2AD-2), the third region sample representative value data (2AD-3), and the fourth region sample representative value data (2AD-4).

[0115] Here, the area value formed by each Raman spectrum (2SP) is a quantitative analysis indicator for each Raman spectrum (2SP), and can be obtained by integrating the region formed by each Raman spectrum (2SP) in the analysis section (B).

[0116] For example, the area value (2A-1) for the Raman spectrum (2SP-1) of the skin surface in the measurement area (400) where the sample is applied may be '5.71369'.

[0117] As such, one embodiment of the present invention calculates the area values ​​of a plurality of Raman spectra (1SP, 2SP) and outputs them as representative values, thereby simplifying the data compared to the linear graph of the Raman spectra (1SP, 2SP) while deriving accurate information (e.g., skin absorption amount and absorption pattern of a target component).

[0118] Continuing, step (d) is a step in which the 3D data generation unit (300) receives the first representative value data (1AD) and the second representative value data (2AD) from the calculation unit (200) and generates a 3D graph so that the amount of skin absorption and the absorption pattern of the target ingredient can be intuitively understood.

[0119] That is, step (d) is a step for generating a color-mapped 3D graph of the skin Raman data (SRD) and sample application data (RRD) obtained in step (a), respectively, to compare and analyze the amount of skin absorption and absorption pattern of the target ingredient after sample application.

[0120] More specifically, step (d) generates a three-dimensional graph (3DG-1) before sample application using the first representative value data (1AD).

[0121] That is, in step (d), the 3D data generation unit (300) generates a 3D graph (3DG-1) before sample application by arranging a plurality of representative values ​​(1A) in the Z-axis direction to correspond to the measured skin depth.

[0122] In addition, step (d) generates a three-dimensional graph (3DG-2) after sample application using the second representative value data (2AD).

[0123] That is, in step (d), the 3D data generation unit (300) generates a 3D graph (3DG-2) after sample application by arranging a plurality of representative values ​​(2A) in the Z-axis direction to correspond to the measured skin depth.

[0124] To explain step (d) in more detail, step (d) comprises: (d-1) a step of inputting representative skin value data of the first region (1AD-1) to the fourth region (1AD-4); (d-2) a step of forming a plurality of three-dimensional data structures (3D-1, 3D-2, 3D-3, 3D-4) by arranging each representative value (1A) of the representative skin value data of the first region (1AD-1) to the fourth region (1AD-4) in the Z-axis direction so as to correspond to the measured skin depth; and (d-3) a step of connecting the plurality of three-dimensional data structures (3D-1, 3D-2, 3D-3, 3D-4) and forming a high-resolution matrix by refining and interpolating the input data. (d-4) A step of forming a three-dimensional graph (3DG-1) before sample application by mapping colors according to a plurality of representative values ​​(1A); (see FIG. 8 (a))

[0125] Additionally, step (d) includes: (d-5) a step of inputting representative value data of a first area sample (2AD-1) to a fourth area sample representative value data (2AD-4); (d-6) a step of forming a plurality of three-dimensional data structures (3D-5, 3D-6, 3D-7, 3D-8) by arranging each representative value (2A) of the representative value data of the first area sample (2AD-1) to the fourth area sample representative value data (2AD-4) in the Z-axis direction so as to correspond to the measured skin depth; and (d-7) a step of connecting the plurality of three-dimensional data structures (3D-5, 3D-6, 3D-7, 3D-8) and forming a high-resolution matrix by refining and interpolating the input data; and (d-8) a step of forming a three-dimensional graph (3DG-2) after sample application by mapping colors according to the plurality of representative values ​​(2A). (See FIG. 8 (b))

[0126] Meanwhile, steps (d-4) and (d-8) can be set differently depending on multiple representative values ​​(1A, 2A).

[0127] A three-dimensional graph (3DG-1, 3DG-2) according to one embodiment of the present invention can be generated to be distinguished by differences in visual brightness corresponding to the magnitude of the calculated representative value.

[0128] For example, areas where the representative values ​​(1A, 2A) are relatively small may be displayed as dark colors (see Fig. 8 (a)).

[0129] As another example, the region where the representative values ​​(1A, 2A) are relatively small can be displayed as a light color (see Fig. 8 (b)).

[0130] Meanwhile, in step (d), the first representative value data (1AD) and the second representative value data (2AD) are input into a Python program installed in the 3D data generation unit (300) so that a 3D graph (3DG-1) before sample application and a 3D graph (3DG-2) after sample application can be generated.

[0131] As described above, according to one embodiment of the present invention, the amount of absorption of a target component according to skin depth can be measured without skin damage using Raman spectroscopy, and the amount of absorption and absorption pattern of the target component in the skin can be intuitively understood by outputting the data obtained through the Raman spectrometer as a three-dimensional graph, thereby having the effect of efficiently analyzing the characteristics of the target component in the sample.

[0132] Although it has been described above that all components constituting an embodiment of the present invention are combined or operate as a single unit, the present invention is not necessarily limited to such an embodiment. That is, within the scope of the purpose of the present invention, all components may be selectively combined in one or more ways to operate.

[0133] The foregoing description is merely an illustrative explanation of the technical concept of the present invention, and those skilled in the art to which the present invention pertains will be able to make various modifications and variations within the scope of the essential characteristics of the present invention. Accordingly, the embodiments disclosed in the present invention are intended to explain, not limit, the technical concept of the present invention, and the scope of the technical concept of the present invention is not limited by these embodiments. The scope of protection of the present invention shall be interpreted by the claims below, and all technical concepts within an equivalent scope shall be interpreted as being included within the scope of rights of the present invention.

Claims

1. A method for analyzing the amount of skin absorption of a target component contained in a sample through a Raman spectrum graph obtained using a Raman spectrometer, (a) A step of obtaining skin Raman data (SRD) including multiple Raman spectra (1SP) by measuring multiple times from a measurement area on the skin surface to a preset measurement depth using the above Raman spectrometer; (b) a step of applying the sample to the measurement area and then measuring multiple times from the measurement area to the measurement depth using the Raman spectrometer to obtain sample application data (RRD) including multiple Raman spectra (2SP); (c) a step of calculating a representative value (1A) for each of a plurality of Raman spectra (1SP) of the skin Raman data (SRD) to generate a first representative value data (1AD) including a plurality of representative values ​​(1A), and calculating a representative value (2A) for each of a plurality of Raman spectra (2SP) of the sample application data (RRD) to generate a second representative value data (2AD) including a plurality of representative values ​​(2A); and (d) a step of generating a 3D graph (3DG-1) before sample application using the first representative value data (1AD) and generating a 3D graph (3DG-2) after sample application using the second representative value data (2AD); A method for analyzing the amount of skin absorption of a sample using Raman spectroscopy, characterized by including 2. In Paragraph 1, The above step (a) is, A method for analyzing the skin absorption amount of a sample using Raman spectroscopy, characterized by obtaining skin Raman data (SRD) by taking multiple measurements along a preset unit interval from the above measurement area to the above measurement depth.

3. In Paragraph 1, (f) a step of setting an analysis interval of the Raman spectrum graph prior to step (a) above; further comprising, The above (f) step is, (f-1) A step of obtaining a Raman spectrum (SP) of the skin surface through the above Raman spectrometer; (f-2) A step of obtaining the Raman spectrum (RP) of the target component through the above Raman spectrometer; and (f-3) A step of comparing the Raman spectrum (SP) of the skin surface and the Raman spectrum (RP) of the target component, and then setting one of the non-overlapping peak sections as the analysis section; A method for analyzing the amount of skin absorption of a sample using Raman spectroscopy, characterized by including 4. In Paragraph 3, The above step (c) is, (c-1) A step of generating the first representative value data (1AD) by setting each area value formed by a plurality of Raman spectra (1SP) of the skin Raman data (SRD) in the analysis section as a plurality of representative values ​​(1A); and (c-2) A step of generating the second representative value data (2AD) by setting each area value formed by the plurality of Raman spectra (2SP) of the sample coating data (RRD) in the analysis section as the plurality of representative values ​​(2A); A method for analyzing the amount of skin absorption of a sample using Raman spectroscopy, characterized by including 5. In Paragraph 1, The above step (d) is, A plurality of the above representative values ​​(1A) are arranged in the Z-axis direction to correspond to the measured skin depth to generate a three-dimensional graph (3DG-1) before the sample application, and A method for analyzing the amount of skin absorption of a sample using Raman spectroscopy, characterized by arranging a plurality of the above representative values ​​(2A) in the Z-axis direction to correspond to the measured skin depth to generate a three-dimensional graph (3DG-2) after the application of the sample.

6. In Paragraph 1, The above step (a) is, (a-1) A step of dividing the above measurement area into a plurality of measurement areas; (a-2) A step of generating multiple skin Raman data (SRD) by measuring each of the multiple measurement regions multiple times up to the measurement depth using the Raman spectrometer; A method for analyzing the amount of skin absorption of a sample using Raman spectroscopy, characterized by including

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