Microfluidic synthesis chip cartridge

Abstract

The present invention relates to a microfluidic synthesis chip cartridge on which a synthesis chip including a microfluidic chamber formed in a predetermined shape is mounted, so that two or more fluids that do not mix well with each other are uniformly mixed to generate a synthesized sample. The purpose of the present invention is to provides a microfluidic synthesis chip cartridge wherein: the microfluidic synthesis chip cartridge includes a plate-shaped member on which two or more synthesis chips having microfluidic channels of a predetermined shape for mixing or reacting two or more liquid samples are mounted in parallel, thereby providing a structure and arrangement capable of dramatically increasing production per unit time despite the synthesis chips having microfluidic channels; and the raw-material supply timings of the two or more synthesis chips are aligned solely by changing the shapes of the microfluidic channels rather than by a separate electronically precise mechanism, thereby enabling synchronization among a plurality of synthesis chips with an inexpensive and simple structure.

Description

Microfluidic synthesis chip cartridge

[0001] The present invention relates to a cartridge equipped with a synthesis chip formed into a microchamber of a predetermined shape to uniformly mix two or more fluids to produce a synthesis sample.

[0002] Microfluidics is a technology that precisely manipulates fluids through microscopic fluid channels and is utilized in various fields, including life sciences, drug development, chemical synthesis, and diagnostic technology. In particular, microfluidic synthesis technology requires high precision and reproducibility in processes such as mixing different fluids within microscopic channels or inducing specific reactions.

[0003] Conventional microfluidic synthesis chips generally feature a structure where only a single microfluidic channel is formed within a single plate (chip) to synthesize samples. In this single-channel approach, synthesis and reaction occur while the fluid maintains a constant flow within the channel, but there is a limitation in that the amount of sample that can be processed per unit time is restricted. Therefore, to increase experimental efficiency or process a large volume of samples simultaneously, multiple chips had to be operated in parallel; however, in this case, there was a problem in that it was difficult to precisely synchronize the fluid flow between individual chips.

[0004] Furthermore, in structures using a single channel, the fluid inflow rate must be strictly controlled to maintain specific reaction conditions, and there is a high probability that reaction times will vary between chips when synthesizing multiple samples simultaneously. To address this, some studies have proposed methods such as utilizing centrifugal force to move the fluid or adding structures to promote mixing within the channel; however, single-channel approaches still face limitations in achieving high throughput.

[0005] When existing microfluidic synthesis chips have a single-channel structure, the following limitations exist.

[0006] First is the limitation of throughput. Conventionally, since a single chip has only one channel, the amount of fluid that can be processed per unit of time was extremely limited. In this case, to increase the experimental speed or synthesize a large amount of fluid, multiple chips had to be operated, which could result in spatial and cost burdens.

[0007] Secondly, there is the difficulty of synthesizing chips. When synthesizing fluids simultaneously using multiple chips, fluid inflow rates and reaction times may vary from chip to chip. In this case, precise electronic control devices are required to maintain uniform flow between chips, but this can increase the complexity of the system.

[0008] Thirdly, there is the issue of reduced uniformity in mixing and reaction. When fluids are synthesized in a single channel, mixing efficiency decreases due to differences in viscosity or flow velocity. To compensate for this, some technologies have introduced turbulent structures within the channel or applied specific fluid distribution methods; however, fundamentally, achieving uniform mixing in a single-channel structure has not been easy.

[0009] Fourth, there is the issue of increased costs and time when scaling up experiments. To conduct experiments on a large scale, a large number of individual chips must be deployed, and each chip must be managed individually. Consequently, there is a problem of increased chip manufacturing costs, experimental costs, and the time required for data collection and analysis.

[0010] [Prior Art Literature]

[0011] (Patent Literature)

[0012] (Patent Document 1) Patent Publication No. 10-2631907 (Date of publication: January 31, 2024)

[0013] Accordingly, the present invention aims to provide a microfluidic synthesis chip cartridge having a structure and arrangement that enables a dramatic increase in production per unit time despite being composed of microchannels, and furthermore, the raw material supply timing of two or more synthesis chips is aligned solely by changing the shape of the microchannels rather than by a separate electronic precision mechanism, thereby enabling synchronization between multiple synthesis chips with an inexpensive and simple structure.

[0014] A microfluidic synthesis chip cartridge according to the present invention for achieving this purpose comprises a plate-shaped member on which a synthesis chip is mounted, having a microchannel of a specific shape formed therein for mixing or reacting two or more liquid samples.

[0015] At this time, the above-mentioned synthetic chips are characterized by being mounted in parallel in two or more places on the plate-shaped member.

[0016] In this case, the above two or more arrangement forms may preferably be characterized by being radially symmetric with respect to the center of the plate-shaped member.

[0017] Here, the plate-shaped member is preferably formed by joining at least two members, and the flow paths through which the two or more liquid samples to be supplied to the synthetic chip are supplied to the synthetic chip may be characterized by each having one flow path independently formed in each of the two or more members.

[0018] In addition, preferably, a gasket is installed between the two or more members, so that the independently formed flow paths are separated from each other by the gasket and airtightness is maintained.

[0019] In this case, the synthetic chip is preferably mounted on any one of the two or more members, and the gasket may be characterized in that a sample passage hole is formed therein for supplying a sample to the synthetic chip through the fluid path that is blocked from the synthetic chip by the gasket.

[0020] Here, the two or more members are preferably an upper block, a middle block, and a lower block joined in sequence.

[0021] And among the two fluids supplied to the synthesis chip, the first sample supply channel is preferably formed on the bottom surface of the upper block, and the second sample supply channel and the synthesis chip may be formed on the top surface of the intermediate block.

[0022] At this time, the intermediate block preferably has a synthetic sample outlet, which is a through hole through which a synthetic sample synthesized with the synthetic chip can fall downward, and the lower block has at least one synthetic sample concentration groove formed therein so that the synthetic sample falling from the synthetic sample outlet is collected in the synthetic sample concentration groove.

[0023] Here, it may be characterized in that preferably two or more synthetic sample outlets are disposed above any one of the synthetic sample concentration grooves.

[0024] In this case, the above-mentioned synthetic sample concentration grooves are preferably formed radially and symmetrically in multiple numbers, and each synthetic sample concentration groove may be characterized by having a synthetic sample discharge pipe formed toward the lower part of the lower block.

[0025] At this time, the above-mentioned synthetic sample concentration groove can preferably be formed in an arc shape where the center of the lower block is the center of curvature.

[0026] In addition, the bottom of the synthetic sample concentration groove may preferably be characterized by being formed as a guiding slope such that the height of the inlet of the synthetic sample discharge pipe is positioned at the lowest level, allowing the synthetic sample collected in the synthetic sample concentration groove to be guided to the synthetic sample discharge pipe.

[0027] Meanwhile, the first sample supply channel and the second sample supply channel are preferably formed in multiple numbers, and the multiple first sample supply channels and second sample supply channels can all be formed radially symmetrically to correspond to the positions of the synthesis chip.

[0028] In this case, the plurality of first sample supply channels preferably receive the first sample from a single supply pipe connected to the upper center of the upper block, and the plurality of second sample supply channels may receive the second sample from a single supply pipe connected to the lower center of the intermediate block.

[0029] Additionally, a fluid alignment zone, which is preferably a section where the cross-sectional area decreases rapidly, is formed in the first sample supply channel or the second sample supply channel, so that even if the first or second sample is supplied first to any one of the plurality of first or second sample supply channels, the supply of samples supplied from all remaining first or second sample supply channels is stopped until they reach the fluid alignment zone, thereby allowing the supplied samples to be injected simultaneously into all of the plurality of synthetic chips.

[0030] Particularly preferably, the fluid alignment zone may be characterized by a decrease in cross-sectional area in such a manner that the bottom side of the flow path gradually rises and approaches the top side of the flow path, thereby allowing the flow of the supplied sample to be stopped until additional pressure is applied from the supply side.

[0031] The microfluidic synthesis chip cartridge according to the present invention has a structure and arrangement that enables a dramatic increase in production per unit time despite being composed of fine channels. Furthermore, since the raw material supply timing of two or more synthesis chips is aligned solely by changing the shape of the fine channels rather than by a separate electronic precision mechanism, it has the effect of enabling synchronization between multiple synthesis chips with an inexpensive and simple structure.

[0032] FIG. 1 is a conceptual diagram of a microfluidic synthesis chip mounted in a microfluidic synthesis chip cartridge according to an embodiment of the present invention.

[0033] FIG. 2 is a perspective view of a microfluidic synthesis chip cartridge according to an embodiment of the present invention.

[0034] FIG. 3a is a side view of a microfluidic synthesis chip cartridge according to an embodiment of the present invention.

[0035] Figure 3b is a perspective view of Figure 3a.

[0036] FIG. 4 is an exploded perspective view of a microfluidic synthesis chip cartridge according to an embodiment of the present invention.

[0037] FIG. 5a is an upper perspective view of the upper block in FIG. 4.

[0038] FIG. 5b is a bottom perspective view of the upper block of FIG. 5a.

[0039] Fig. 6a is an upper perspective view of the middle block in Fig. 4.

[0040] Fig. 6b is a bottom perspective view of the middle block of Fig. 6a.

[0041] FIG. 7a is an upper perspective view of the lower block in FIG. 4.

[0042] Fig. 7b is a bottom perspective view of the lower block of Fig. 7a.

[0043] Figure 8 is a partial enlarged view of Figure 6a.

[0044] Figure 9a is a conceptual diagram showing the flow paths of two raw material liquids supplied to the synthesis chip.

[0045] Figure 9b is a conceptual diagram that further simplifies Figure 9a.

[0046] Fig. 9c is a plan view of the gasket in Fig. 9a.

[0047] Figure 10a is a diagram showing the fluid alignment zone in Figure 8.

[0048] Figure 10b is a cross-sectional view of line AA' in Figure 10a.

[0049] FIG. 11a is a bottom view of the lower block.

[0050] Figure 11b is a cross-sectional view in the width direction of the fluid concentration groove in Figure 11a.

[0051] FIG. 11c is a longitudinal cross-sectional view of the fluid concentration groove in FIG. 11a.

[0052] The present invention will be described in detail below with reference to the attached drawings.

[0053] Referring to FIGS. 2 to 4, a microfluidic synthesis chip cartridge according to one embodiment of the present invention is composed of a plate-shaped member on which a synthesis chip (33) is mounted with a microchannel of a certain shape formed for mixing or reacting two or more liquid samples.

[0054] Here, the composite chip (33) is characterized by having two or more chips mounted in parallel on a plate-shaped member.

[0055] For reference, the synthetic chip (33) is identical to the synthetic chip (3) exemplarily illustrated in FIG. 1 and is a microfluidic chamber formed in a unique shape to allow two or more types of fluids that are difficult to mix with each other, such as a hydrophobic liquid and a hydrophilic liquid, to be mixed highly uniformly. More specifically, the synthetic chip (33) has a width-expanding section and a bottleneck section repeated a certain number of times, and a rectangular prism-shaped obstacle is placed in the width-expanding section to form a vortex in the incoming fluid. The ratio of the width and length of the width-expanding section and the number of bottleneck sections, as well as the size and width of the rectangular prism-shaped obstacle, are all formed in a ratio optimized to obtain the highest synthesis efficiency. However, since the synthetic chip itself is a known technology, further detailed explanation will be omitted.

[0056] The synthesis chip (33) has the effect of uniformly mixing liquids that are difficult to mix with each other with excellent efficiency, but there is a limit to the output per unit time as it consists of fine channels.

[0057] In this regard, in the present invention, two or more composite chips (33) are arranged in parallel on a plate-shaped member, and a sample tube supplied to the composite chips (33) arranged in parallel is provided for each sample, thereby dramatically improving the efficiency of producing composite samples per unit time.

[0058] At this time, referring to FIG. 4 and FIG. 6a, the arrangement of two or more composite chips (33) is characterized by being radially symmetric with respect to the center of the plate-shaped member. Thus, a single supply pipe can be connected to multiple composite chips (33) simultaneously in a small area.

[0059] Here, the plate-shaped member is formed by joining at least two members, and the flow paths through which two or more liquid samples to be supplied to the synthetic chip (33) are supplied to the synthetic chip are independently formed, with one flow path for each of the two or more members. This will be described in detail later.

[0060] When two or more liquid samples are supplied to a flow path formed in each member joined together, the two samples must each be kept airtight until they are introduced into the synthetic chip (33). To this end, a gasket (20) of FIG. 9c, which will be described later, is installed between the members, so that the independently formed flow paths can be separated from each other by the gasket (20) and kept airtight.

[0061] The synthetic chip (33) may be mounted on any one of the two or more members. At this time, the supply sample formed on the member on the side where the synthetic chip (33) is not mounted and the synthetic chip (33) need to be in communication with each other even though the two members are isolated by a gasket (20).

[0062] To this end, as shown in FIG. 9c, a first sample passage hole (22) may be formed in the gasket (20) to supply a sample to the synthetic chip (33), through a flow path that is blocked from the synthetic chip (33) by the gasket (20).

[0063] Meanwhile, more specifically, the two or more members may be an upper block (10), an intermediate block (30), and a lower block (40) that are joined in sequence as shown in FIGS. 3a to 4.

[0064] At this time, among the two fluids supplied to the synthetic chip (33), the first sample supply channel (121) is formed on the bottom surface (12) of the upper block, and the second sample channel (333) and the synthetic chip (33) may be formed on the upper surface (31) of the middle block. However, the form in which the synthetic chip (33) is formed on the upper block (10) rather than the middle block (30) is not necessarily excluded in the present invention.

[0065] The sample supply channel formed in the upper block (10) is a first sample supply channel (121) formed on the bottom surface (12) of the upper block as shown in FIG. 5b. At this time, the first sample supply channel (121) is connected to a first sample inlet so that it can be supplied to the synthetic chip (33) shown in FIG. 6a through the first sample passage hole (22) of the gasket (20) shown in FIG. 9c. And the first sample (A) supplied to the first sample supply channel (121) is injected into the first sample injection hole (14) formed in the center of the upper block (10), with reference to FIG. 5a, FIG. 5b, and FIG. 9a.

[0066] Then, the first sample (A), which is transferred to the first sample supply channel (121) formed on the bottom surface (12) of the upper block shown in FIG. 5b, enters the first sample inlet (331) formed on the upper surface (31) of the middle block through the first sample passage hole (22) formed in the gasket (20), and then moves through the first sample channel (332) also formed on the upper surface (31) of the middle block to be transferred to the synthetic chip (33).

[0067] A second sample channel (333) for delivering a second sample (B) to a synthetic chip (33) and a second sample channel (333) for delivering a second sample (B) to the synthetic chip (33) is formed on the upper surface (31) of the intermediate block as shown in FIG. 6a. A second sample injection hole (34) is formed at the center of the intermediate block (30) and at the center of the lower block (40), which will be described later, so that the second sample (B) can be supplied to the second sample channel (333).

[0068] The second sample channel (333) can be formed such that two channels are connected to each side of a single synthetic chip (33). That is, two second sample channels (333) extending from the second sample injection hole (34) are connected to a single synthetic chip (33). This is to ensure that the second sample (B) is uniformly supplied to the starting point of the synthetic chip (33), even though the second sample channel (333) cannot be connected to the center of the synthetic chip (33) in order to be spaced apart from the first sample channel (332).

[0069] Additionally, with reference to FIGS. 6a and 6b, a synthetic sample outlet (35), which is a through hole through which a synthetic sample (C) synthesized with a synthetic chip (33) can fall downward, is formed in the middle block (30). Correspondingly, with reference to FIG. 7a, a plurality of synthetic sample concentration grooves (43) can be formed on the upper surface (41) of the lower block so that a synthetic sample (C) falling from above can be collected.

[0070] At this time, two or more synthetic sample outlets (35) may be arranged above one synthetic sample concentration groove (43). Therefore, even if the amount of synthetic sample (C) falling through one synthetic sample outlet (35) is small, the amount of synthetic sample (C) collected through two or more synthetic sample outlets (35) can reach at least several liters per hour, so the synthetic sample (C) can be smoothly discharged by its own weight even without a separate means such as an extraction pump.

[0071] At this time, for reference, a synthetic sample discharge port (433) is formed in the synthetic sample concentration groove (43) so that the collected synthetic sample (C) can fall by its own weight, and a synthetic sample discharge pipe (44) connected to the synthetic sample discharge port (433) may be provided as shown in FIG. 7b.

[0072] A plurality of synthetic sample concentration grooves (43) may be formed radially and symmetrically. In particular, the synthetic sample concentration grooves (43) are formed in an arc shape with the center of the lower block (40) as the center of curvature, so that the angle at which the lower block (40) is coupled to the intermediate block (30) can be selected in various ways, and regardless of the angle at which they are coupled, the synthetic sample outlet (35) can be located at the center of the synthetic sample concentration grooves (43), making the coupling between the intermediate block (30) and the lower block (40) much easier.

[0073] Additionally, the bottom of the synthetic sample concentration groove (43) can be formed as a guiding slope (431) such that the synthetic sample (C) collected in the synthetic sample concentration groove (43) can be guided to the synthetic sample discharge pipe (44) by positioning the inlet height of the synthetic sample discharge pipe (44) at the lowest level, that is, by forming the position of the synthetic sample discharge port (433) at the lowest level.

[0074] Referring to FIG. 11b and FIG. 11c, two guiding slopes (431) form the bottom of the synthetic fluid concentration groove (43). At this time, the guiding slopes (431) are formed along the longitudinal direction of the synthetic fluid concentration groove (43), so that the synthetic sample (C) collected in the synthetic fluid concentration groove (43) can be gathered at the synthetic sample outlet (433). In particular, as shown in FIG. 11c, the slope is formed so that the synthetic sample outlet (433) is at the lowest position along the longitudinal direction of the synthetic sample concentration groove (43), so that the collected synthetic sample (C) can be immediately gathered at the synthetic sample outlet (433).

[0075] Meanwhile, referring to FIGS. 9a to 10b, it can be seen that the first sample supply channel, i.e., the first sample supply channel (121), and the second sample supply channel, i.e., the second sample channel (333), are formed in multiple numbers, and the multiple first sample supply channels (121) and second sample channels (333) are all formed radially symmetrically to correspond to the positions of the synthetic chip (33).

[0076] Here, a plurality of first sample supply channels (121) receive a first sample (A) from a supply pipe connected to the upper center of the upper block, and a plurality of second sample channels (333) receive a second sample (B) from a supply pipe connected to the lower center of the middle block (30). Here, as shown in FIG. 9a, a first sample supply module (1) can be connected to a first sample injection hole (14) which is the center of the upper block (10), and a second sample supply module (2) can be connected to a second sample injection hole (34) which is the center of the middle block (30).

[0077] At this time, a fluid alignment zone (334), which is a section in which the cross-sectional area decreases rapidly, may be formed in the first sample channel (332) or the second sample channel (333) that is connected to the first sample supply channel (121) and the first sample passage hole (22).

[0078] Although FIGS. 9a and 9b show that a fluid alignment zone (334) is formed in the second sample channel (333), a fluid alignment zone may also be formed in the first sample channel (332) depending on the case, that is, depending on the viscosity or supply amount according to the type of the first sample (A) supplied. Hereinafter, it will be described as if a fluid alignment zone (334) is formed in the second sample channel (333).

[0079] The second sample channel (333) is formed such that the large diameter section (3331) and the capillary section (3332) are connected to the fluid alignment zone (334), so that even if the second sample (B) is first supplied to any one of the multiple second sample channels (333), the supply of the sample is stopped until the second sample (B) supplied to all remaining second sample channels (333) reaches the fluid alignment zone (334).

[0080] This is because, even if the second sample (B) enters one of the second sample channels (333) first, if the second sample (B) reaches the fluid alignment zone (334) of one of the second sample channels (333) first—that is, if the second sample (B) has advanced only to the large diameter section (3331) of one of the second sample channels (333)—the cross-sectional area of ​​the flow path in the fluid alignment zone (334) decreases rapidly. Therefore, the supply pressure of the second sample (B) injected into the second sample injection hole (34) is first used to advance the second sample (B) in another second sample channel (333) where the second sample (B) has not yet reached the fluid alignment zone (334). Thus, the process of the supplied sample entering multiple synthetic chips (33) can be carried out simultaneously in all synthetic chips (33).

[0081] In particular, referring to FIG. 10b, the fluid alignment zone (334) has a cross-sectional area that decreases as the bottom side of the starting point of the capillary section (3332) gradually rises and approaches the top side of the capillary section (3332), so that the supply sample stops moving until additional pressure is applied from the supply side.

[0082] Even if a fluid alignment zone (334) is formed and the diameter of the second sample channel (333) decreases rapidly, if the bottom of the fluid alignment zone (334) is at the same height as or similar to the bottom of the second sample channel (333), that is, if the bottom of the capillary section (3332) and the bottom of the large diameter section (3331) are at the same height as or similar to each other, then due to residual pressure, a portion of the second fluid (B) can pass over the fluid alignment zone (334) and enter the capillary section (3332) with only a small amount of force.

[0083] In contrast, as shown in FIG. 10b, if the fluid alignment zone (334) is reduced in a form where the cross-sectional area decreases in such a way that the ceiling of the cross-section decreases rather than the floor height increases, then the second sample (B) that has reached the fluid alignment zone can remain in a stagnant state in the fluid alignment zone (334) until all the second samples (B) from all other second sample channels (333) have reached the fluid alignment zone (334) and all have reached the stage where they can pass through the fluid alignment zone (334) only when a greater pressure is applied, even if a certain extra pressure is applied. Ultimately, all the second samples (B) in all capillary sections (3332) can enter the synthesis chip simultaneously.

[0084] The present invention described above is not limited by the aforementioned embodiments and attached drawings, and it will be obvious to those skilled in the art that various substitutions, modifications, and changes are possible within the scope of the technical concept of the present invention.

[0085] [Explanation of the symbol]

[0086] A: Sample 1 B: Sample 2

[0087] C: Synthetic Sample 1: First Sample Supply Module

[0088] 2: Second sample supply module 3: Synthesis chip

[0089] 10: Upper block 11: Upper block top surface

[0090] 12: Bottom of upper block 13: Upper block fastening hole

[0091] 14: First sample injection hole 20: Gasket

[0092] 21: Gasket fastening hole 22: First sample passage hole

[0093] 23: 1st O-ring 30: Middle block

[0094] 31: Middle block top surface 32: Middle block bottom surface

[0095] 33: Synthesis chip 34: Second sample injection hole

[0096] 35: Synthetic sample outlet 36: Seal wall insertion groove

[0097] 37: Second O-ring 38: Intermediate block fastening hole

[0098] 40: Lower block 41: Upper surface of lower block

[0099] 42: Bottom of lower block 43: Synthetic sample concentration groove

[0100] 44: Synthetic sample discharge pipe 45: Lower block fastening hole

[0101] 50: Fastening member 121: First sample supply channel

[0102] 331: First sample inlet 332: First sample channel

[0103] 333: Second sample channel 334: Fluid alignment zone

[0104] 431: Induction slope 432: Sealing wall

[0105] 433 : Synthetic sample outlet 3331 : Large diameter section

[0106] 3332 : Moses Section

Claims

1. A plate-shaped member mounted on a synthetic chip having a microchannel of a specific shape formed therein for mixing or reacting two or more liquid samples; comprising, Two or more of the above-mentioned synthetic chips are mounted in parallel on the plate-shaped member, and The above two or more arrangement forms are radially symmetric with respect to the center of the plate-shaped member, and The above plate-shaped member is formed by joining at least two members, and the flow paths through which the two or more liquid samples to be supplied to the synthesis chip are supplied to the synthesis chip are each independently formed as one flow path in each of the two or more members. The above two or more members are an upper block, a middle block, and a lower block that are joined in sequence, and Among the two fluids supplied to the synthetic chip cartridge, the first sample supply channel is formed on the bottom surface of the upper block, and the second sample supply channel and the synthetic chip are formed on the top surface of the intermediate block. A microfluidic synthesis chip cartridge characterized in that the intermediate block has a synthesis sample outlet formed as a through hole through which a synthesis sample synthesized by the synthesis chip can fall downward, and the lower block has at least one synthesis sample concentration groove formed so that the synthesis sample falling from the synthesis sample outlet is collected in the synthesis sample concentration groove.

2. In Paragraph 1, A microfluidic synthesis chip cartridge characterized by having a gasket installed between the two or more members, wherein the independently formed flow paths are separated from each other by the gasket to maintain airtightness.

3. In Paragraph 2, The synthetic chip is mounted on any one of the two or more of the above components, and A microfluidic synthesis chip cartridge characterized in that the above gasket has a sample passage hole formed therein for supplying a sample to the synthesis chip through the above flow path, which is blocked from the synthesis chip by the gasket.

4. In Paragraph 1, A microfluidic synthesis chip cartridge characterized by having two or more synthesis sample outlets disposed above one of the synthesis sample concentration grooves.

5. In Paragraph 4, A microfluidic synthesis chip cartridge characterized by a plurality of synthesis sample concentration grooves being formed radially and symmetrically, and each synthesis sample concentration groove being provided with a synthesis sample discharge pipe formed toward the lower part of the lower block.

6. In Paragraph 5, A microfluidic synthesis chip cartridge characterized in that the above-mentioned synthesis sample concentration groove is formed in an arc shape where the center of the lower block is the center of curvature.

7. In Paragraph 5, A microfluidic synthesis chip cartridge characterized in that the bottom of the synthesis sample concentration groove is formed as a guiding slope so that the inlet height of the synthesis sample discharge pipe is positioned at the lowest level, allowing the synthesis sample collected in the synthesis sample concentration groove to be guided to the synthesis sample discharge pipe.

8. In Paragraph 1, The first sample supply channel and the second sample channel are formed in multiple numbers, and the multiple first sample channels and second sample channels are all formed radially symmetrically to correspond to the positions of the synthesis chip. A plurality of the first sample channels receive a first sample from a single supply pipe connected to the upper center of the upper block, and a plurality of the second sample channels receive a second sample from a single supply pipe connected to the lower center of the intermediate block. In the first sample channel or the second sample channel, a fluid alignment zone is formed, which is a section where the cross-sectional area decreases rapidly, thereby, Even if a first or second sample is first supplied to any one of the plurality of first or second sample channels, the supply of samples is stopped until the samples supplied from all remaining first or second sample channels reach the fluid alignment zone, A microfluidic synthesis chip cartridge characterized by the fact that the supplied sample is injected simultaneously into multiple synthesis chips.

9. In Paragraph 8, A microfluidic synthesis chip cartridge characterized by the above-mentioned fluid alignment zone having a reduced cross-sectional area in such a way that the bottom side of the fluid path gradually rises and approaches the top side of the fluid path, thereby allowing the flow of the supplied sample to be stopped until additional pressure is applied from the supply side.

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