The invention provides a display panel, a manufacturing method of the display panel and a display device. The display panel comprises a first substrate assembly, a second substrate assembly and a frame sealing assembly located between the first substrate assembly and the second substrate assembly, the frame sealing assembly comprises at least one first electrode, at least one second electrode and a frame sealing glue layer located between the first electrode and the second electrode, and particle media are arranged in the frame sealing glue layer. The first electrode is arranged on the side, opposite to the first substrate assembly, of the frame sealing adhesive layer, the second electrode is arranged on the side, opposite to the second substrate assembly, of the frame sealing adhesive layer, the first electrode and the second electrode are used for forming different preset electric fields, and the particle medium deflects or stretches under the action of the preset electric fields so as to adjust the thickness of the frame sealing adhesive layer. The thickness of the display area and the thickness of the non-display area of the display panel are matched, the situation that the display panel is uneven in display is avoided, and the display quality of the display panel is improved.