Rapid breeding method based on tissue culture for helleborus thibetanus

A technique of tissue culture and iron chopsticks, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of large market demand, limited supply of seedlings, low germination rate, etc., and achieve the maintenance of female parent traits and low cost The effect of reducing and increasing the reproduction speed

CN107864855AInactive Publication Date: 2018-04-03上海上房园艺有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2018-04-03
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention relates to a rapid breeding method based on tissue culture for helleborus thibetanus. The method comprises the following steps: (1) preparing a bud inducing culture medium, an adventitious bud proliferation culture medium, a seedling strengthening culture medium, and a rooting culture medium; and (2) performing tissue culture on the helleborus thibetanus. According to the method provided by the invention, through a tissue culture technology, the annual propagation coefficient is improved to 1:20000 and the cost is reduced by 0.8-1.0 yuan / plant compared with those of the prior art; and the propagation speed and seedling uniformity are greatly improved through tissue culture seedlings of the helleborus thibetanus, and original female parent characters are better maintained.
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Description

technical field

[0001] The invention relates to a method for rapid plant propagation, in particular to a method for rapid propagation of iron chopsticks based on tissue culture. Background technique

[0002] Iron chopsticks is a plant of the genus Iron chopsticks in the family Ranunculaceae. It is a perennial evergreen herb with kidney-shaped or pentagonal leaves, three-segmented chicken feet, cauline leaves that are nearly sessile, and basal leaves that are smaller. Flowers on stems or branches, open when basal leaves just emerge, glabrous; sepals are initially pink and turn green during fruiting. The flowering period is March-April, and the fruiting period is May. Because of its unique flower shape, iron chopsticks bloom in early spring, have few pests and diseases, and have strong shade tolerance. They can be planted in large areas and are excellent materials for understory ground cover plants. As an excellent native garden plant, iron chopsticks are gradually recognize...

Examples

Embodiment 1

[0034] A method for rapidly breeding iron chopsticks based on tissue culture, comprising the following steps:

[0035] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0036] 1.1. Bud induction medium: MS+BA 3mg / L+NAA 0.3mg / L;

[0037] 1.2. Adventitious bud proliferation medium: MS+6-BA 2.0mg / L+NAA 0.2mg / L;

[0038] 1.3. Strong seedling medium: MS+6-BA 1.0mg / L+NAA 0.1mg / L;

[0039] 1.4. Rooting medium: MS+NAA 0.2mg / L+IBA 0.2mg / L;

[0040] The pH of the above-mentioned various media is 5.8, add 30g / L sucrose, 6g / L agar, culture temperature is 22°C, light is 2000Lx;

[0041] (2) Tissue culture of iron chopsticks:

[0042] 2.1. Obtaining sterile materials

[0043] Iron chopsticks dig out the small buds above the ground in March in spring, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for ...

Embodiment 2

[0053] A method for rapidly breeding iron chopsticks based on tissue culture, comprising the following steps:

[0054] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0055] 1.1. Bud induction medium: MS+BA 1mg / L+NAA 0.1mg / L;

[0056] 1.2. Adventitious bud proliferation medium: MS+6-BA 1.0mg / L+NAA 0.1mg / L;

[0057] 1.3. Strong seedling medium: MS+6-BA 0.5mg / L+NAA 0.1g / L;

[0058] 1.4. Rooting medium: MS+NAA 0.1mg / L+IBA 0.1mg / L;

[0059]The pH of the above-mentioned various media is 5.8, add 30g / L sucrose, 6g / L agar, culture temperature is 21°C, light is 1900Lx;

[0060] (2) Tissue culture of iron chopsticks:

[0061] 2.1. Obtaining sterile materials

[0062] Iron chopsticks dig out the small buds above the ground in March in spring, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15...

Embodiment 3

[0072] A method for rapidly breeding iron chopsticks based on tissue culture, comprising the following steps:

[0073] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0074] 1.1. Bud induction medium: MS+BA 5mg / L+NAA 0.5mg / L;

[0075] 1.2. Adventitious bud proliferation medium: MS+6-BA 3.0mg / L+NAA 0.3mg / L;

[0076] 1.3. Strong seedling medium: MS+6-BA 1.5mg / L+NAA 0.2mg / L;

[0077] 1.4. Rooting medium: MS+NAA 0.3mg / L+IBA 0.3mg / L;

[0078] The pH of the above various media is 5.8, add 30g / L sucrose, 6g / L agar, culture temperature is 23°C, light is 2100Lx;

[0079] (2) Tissue culture of iron chopsticks:

[0080] 2.1. Obtaining sterile materials

[0081] Iron chopsticks dig out the small shoots of the aboveground part in March in spring, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 1...