Preparation method of autophagy-imitated immune cell supported antitumor therapeutic agent

An immune cell and anti-tumor technology, which is applied in the field of preparation of immune cell-loaded anti-tumor therapeutic agents, can solve the problems of ineffective control of irregular release of materials, poor dispersion, and low phagocytosis, and achieve good drug slow-release behavior, The effect of improving load and good migration ability

Active Publication Date: 2018-11-16
TONGJI UNIV
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Problems solved by technology

For example, the photosensitive material Ce6 is difficult to dissolve in water, and it is easy to form a conjugated structure between molecules, resulting in poor dispersion. After co-incubating with cells, the amount o

Method used

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  • Preparation method of autophagy-imitated immune cell supported antitumor therapeutic agent
  • Preparation method of autophagy-imitated immune cell supported antitumor therapeutic agent
  • Preparation method of autophagy-imitated immune cell supported antitumor therapeutic agent

Examples

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Embodiment 1

[0027] A method for preparing an autophagy-imitating immune cell-loaded anti-tumor therapeutic agent, using the following steps:

[0028] (1) A certain number of macrophages were treated with Tris-MgCl 2 The buffer was frozen and thawed several times, passed through a mini-extruder (40nm-100nm), added 0.5-1M Sucrose (final concentration 0.125-0.25M), centrifuged (800-2000g, 5-15min), took the upper layer, and centrifuged again (2000-3000g , 30~40min), remove the lower layer, and add Tris~MgCl 2 buffer (containing 0.125 ~ 0.25M sucrose) wash, and then twice H 2 O washed, collected and freeze-dried.

[0029] (2) Weigh 3-4 mg of cell membrane fragments from the freeze-dried sample in step (1) and dissolve them in PBS (1X); take 8-10 mg of Ce6, add them to the cell membrane suspension, and ultrasonically pulverize (the control power is 80-150 W, each time ultrasonic 5-10s, the total duration of ultrasound is 5-10min). After the sonication is over, take out the solution at room...

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Abstract

The invention relates to a preparation method of an autophagy-imitated immune cell supported antitumor therapeutic agent. The preparation method comprises steps as follows: an antitumor therapeutic agent, cell membranes and immune cells are taken as raw materials, immune cell membranes are extracted to encapsulate the antitumor therapeutic agent, nano-particles containing apoptosis groups are co-cultured with the immune cells after being formed, the nano-particles are phagocytized by the immune cells, the antitumor therapeutic agent is enabled to be indirectly encapsulated into the immune cells, and the autophagy-imitated immune cell supported antitumor therapeutic agent is prepared. Compared with the prior art, the antitumor therapeutic agent is encapsulated with the cell membranes containing the apoptosis groups, the phagocytosis quantity of the antitumor therapeutic agent by the immune cells is increased, cell carriers meeting various demands are prepared, the problems of irregularrelease and low phagocytosis quantity of the therapeutic agent due to adoption of a traditional supporting method are solved, and meanwhile, toxicity of drugs for the cell carriers is reduced.

Description

technical field [0001] The invention belongs to the field of nanomedicine, and in particular relates to a preparation method of immune cells imitating autophagy loaded with an anti-tumor therapeutic agent. Background technique [0002] Live cell drug delivery has broad application prospects in biomedical engineering, materials science, pharmacy and other fields. In the past few years, live cell delivery was mainly formed by co-incubation of living cells with drug-loaded nanoparticles or drugs. For example, Jinhyang Choi et al. used mouse macrophages to load contrast diagnostic agents; Wen-Chia Huang et al. used monocyte-macrophages to load nanoparticles loaded with chemotherapeutic drugs. This loading method produces more toxicity to cells on the one hand, and on the other hand, the loading amount is relatively low. [0003] For a long time, anti-tumor therapeutic agents are generally encapsulated with polymer materials such as liposomes and polymer nanoparticles to reduce ...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/46A61K41/00A61P35/00
CPCA61K9/5068A61K41/0071A61P35/00
Inventor 李永勇王怀基董海青
Owner TONGJI UNIV
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