Biological indicator based on phosvitin as well as preparation method and application of biological indicator

Abstract

The invention belongs to the technical field of sterilization indication, and particularly relates to a biological indicator based on phosvitin. The biological indicator is a phosvitin aqueous solution or a phosvitin aqueous solution containing a phosvitin iron compound. The invention provides a preparation method and application of the biological indicator based on phosvitin. According to the biological indicator based on phosvitin, the problem that monitoring of the steam sterilization effect is blank is solved, biological culture is not needed, the conclusion whether the sterilization effect is qualified or not can be obtained in time, the using and issuing period of sterilization instruments is shortened, the distribution and use efficiency of sterilization instruments is improved, the monitoring cost is low, and the biological indicator is suitable for sterilization monitoring of food products.

Description

technical field

[0001] The invention belongs to the technical field of sterilization indicators, and in particular relates to a biological indicator based on phosvitin, a preparation method and an application thereof. Background technique

[0002] As food safety has been widely concerned, the sterilization requirements for food and fast food products have become increasingly stringent. At present, the main sterilization methods include high-temperature and high-pressure steam, high-temperature dry heat, and ultraviolet sterilization.

[0003] High-pressure steam sterilization is a commonly used sterilization method. It releases a large amount of latent heat through steam for sterilization. It is economical, effective, safe, fast, non-residual, and non-toxic. However, during the steam sterilization process, it is difficult for the steam to penetrate the sample. For the items in the slit, simply monitoring according to the common standard of sterilization at 121°C for 15 minut...

Examples

preparation example Construction

[0038] A preparation method of a biological indicator based on phosvitin, comprising the steps of:

[0039]Take fresh eggs, use an egg separator to separate the egg white and egg yolk, take the egg yolk and place it on the filter paper, roll the egg yolk to remove the excess ligament and egg white, pierce the egg yolk membrane with a bamboo stick, and collect the egg yolk liquid; Add 0.8 to 1.5 times the volume of distilled water to dilute the egg yolk liquid, centrifuge at 3500×g for 30 minutes to collect the lower sediment; Centrifuge for 30 minutes to collect the precipitate; add pH regulators HCl and NaOH to homogeneously precipitate at pH 11.3 to 11.5 for 5 to 10 minutes, add 3 times the volume of water, and centrifuge at 3500×g for 30 minutes to collect the supernatant; use Masterflex I / P ultrafiltration equipment, 30KDa ultrafiltration membrane, first pass through 0.22μm membrane suction filtration, then ultrafiltration at room temperature to remove the salt in the supe...

Embodiment 1

[0047] A preparation method of a biological indicator based on phosvitin, comprising the steps of:

[0048] Take fresh eggs, use an egg separator to separate the egg white and egg yolk, take the egg yolk and place it on the filter paper, roll the egg yolk to remove the excess ligament and egg white, pierce the egg yolk membrane with a bamboo stick, and collect the egg yolk liquid; Add 1 times the volume of distilled water to dilute the egg yolk liquid, centrifuge at 3500×g for 30 minutes to collect the lower layer sediment; homogenize the lower layer with double distilled water with 5 times the volume of the sediment, and centrifuge at 3500×g for 30 minutes to collect Precipitate; add pH regulator HCl and / or NaOH, homogeneously precipitate at pH 11.4 for 10 minutes, add 3 times the volume of water, and centrifuge at 3500×g for 30 minutes to collect the supernatant; remove the supernatant by ultrafiltration After adding salt, centrifuge at 3500×g for 30 minutes to collect the s...

Embodiment 2

[0051] Present embodiment 2 is on the basis of embodiment 1, further performs the following operation steps:

[0052] The aqueous solution of phosvitin was sonicated, and then 7 μg / mL of FeCl was added 3 Form a phosvitin-iron complex to obtain a biological indicator based on phosvitin; the ultrasonic power of the ultrasonic treatment is 200W / cm 2 , sonication time is 15min.

technical field

[0001] The invention belongs to the technical field of sterilization indicators, and in particular relates to a biological indicator based on phosvitin, a preparation method and an application thereof. Background technique

[0002] As food safety has been widely concerned, the sterilization requirements for food and fast food products have become increasingly stringent. At present, the main sterilization methods include high-temperature and high-pressure steam, high-temperature dry heat, and ultraviolet sterilization.

[0003] High-pressure steam sterilization is a commonly used sterilization method. It releases a large amount of latent heat through steam for sterilization. It is economical, effective, safe, fast, non-residual, and non-toxic. However, during the steam sterilization process, it is difficult for the steam to penetrate the sample. For the items in the slit, simply monitoring according to the common standard of sterilization at 121°C for 15 minut...

Examples

preparation example Construction

[0038] A preparation method of a biological indicator based on phosvitin, comprising the steps of:

[0039]Take fresh eggs, use an egg separator to separate the egg white and egg yolk, take the egg yolk and place it on the filter paper, roll the egg yolk to remove the excess ligament and egg white, pierce the egg yolk membrane with a bamboo stick, and collect the egg yolk liquid; Add 0.8 to 1.5 times the volume of distilled water to dilute the egg yolk liquid, centrifuge at 3500×g for 30 minutes to collect the lower sediment; Centrifuge for 30 minutes to collect the precipitate; add pH regulators HCl and NaOH to homogeneously precipitate at pH 11.3 to 11.5 for 5 to 10 minutes, add 3 times the volume of water, and centrifuge at 3500×g for 30 minutes to collect the supernatant; use Masterflex I / P ultrafiltration equipment, 30KDa ultrafiltration membrane, first pass through 0.22μm membrane suction filtration, then ultrafiltration at room temperature to remove the salt in the supe...

Embodiment 1

[0047] A preparation method of a biological indicator based on phosvitin, comprising the steps of:

[0048] Take fresh eggs, use an egg separator to separate the egg white and egg yolk, take the egg yolk and place it on the filter paper, roll the egg yolk to remove the excess ligament and egg white, pierce the egg yolk membrane with a bamboo stick, and collect the egg yolk liquid; Add 1 times the volume of distilled water to dilute the egg yolk liquid, centrifuge at 3500×g for 30 minutes to collect the lower layer sediment; homogenize the lower layer with double distilled water with 5 times the volume of the sediment, and centrifuge at 3500×g for 30 minutes to collect Precipitate; add pH regulator HCl and / or NaOH, homogeneously precipitate at pH 11.4 for 10 minutes, add 3 times the volume of water, and centrifuge at 3500×g for 30 minutes to collect the supernatant; remove the supernatant by ultrafiltration After adding salt, centrifuge at 3500×g for 30 minutes to collect the s...

Embodiment 2

[0051] Present embodiment 2 is on the basis of embodiment 1, further performs the following operation steps:

[0052] The aqueous solution of phosvitin was sonicated, and then 7 μg / mL of FeCl was added 3 Form a phosvitin-iron complex to obtain a biological indicator based on phosvitin; the ultrasonic power of the ultrasonic treatment is 200W / cm 2 , sonication time is 15min.

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