Microwell array chip and single cell sorting method based on laser-induced forward transfer

By combining LIFT technology on the microporous array chip, efficient and accurate single-cell capture and sorting in liquids is achieved, and the problem that single-cell sorting in the prior art is difficult to achieve in liquids is solved, which improves the sorting success rate and reduces thermal damage.

CN115198376BActive Publication Date: 2025-05-13CHANGCHUN CHANGGUANG CHENYING BIOSCIENCE INSTR CO LTD
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Patent Information

Application Number
CN202210543398.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-05-18
Publication Date
2025-05-13
Estimated Expiration
2042-05-18

AI Technical Summary

Technical Problem

The existing single-cell sorting technology is difficult to achieve precise capture and reduce sorting damage in liquids, resulting in a low success rate of single-cell live sorting.

Method used

Micropore array chips combined with laser-induced forward transfer (LIFT) technology are used to cover biocompatible micropore membranes on metal-coated glass sheets to achieve the formation and fixation of single-cell microdroplets, thereby accurately capturing and sorting of single-cell cells.

Benefits of technology

It realizes efficient and accurate single-cell capture and sorting in liquid, reduces thermal damage during the sorting process, and improves the success rate of single-cell living organism sorting.

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Abstract

The present invention provides a microporous array chip and a single cell sorting method for laser-induced forward transfer. A layer of microporous membrane is covered on a metal-coated glass sheet. The microporous membrane is a biocompatible thin film and is covered on the metal-coated glass sheet using a micro-nano processing technology. Due to the hydrophobic properties of the microporous array chip material, a single cell forms a single cell micro-droplet, which is fixed in the micropores to achieve single cell capture, facilitating subsequent single cell sorting. The stability and signal-to-noise ratio of the liquid single cell Raman spectrum are greatly increased, laying a foundation for single cell liquid Raman identification and sorting.
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