Preparation method of cerium oxide micro-nano particles with a multi-level structure and its application in detection and identification of small molecule metabolites

Through multi-level structured cerium oxide micro-nano particles as mass spectrometry matrix materials, the problems of background interference and hot spot effects in small molecule metabolites detection are solved, and low-cost and simple-step detection methods are realized, meeting the needs of obtaining clinical metabolic fingerprint maps.

CN115372454BActive Publication Date: 2025-06-24SHANGHAI JIAOTONG UNIV
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Patent Information

Application Number
CN202210874046.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-07-21
Publication Date
2025-06-24
Estimated Expiration
2042-07-21

AI Technical Summary

Technical Problem

The prior art has problems of background interference and hot spot effects in the detection and identification of small molecule metabolites, and the sample preprocessing process is complex and it is difficult to meet the needs of clinical applications.

Method used

Multi-stage structure cerium oxide micro-nano particles are used as the matrix material in the mass spectrometry, and cerium oxide micro-nano particles are prepared by template method, and used as matrix solution in laser desorption ionization mass spectrometry detection to simplify the sample processing process.

Benefits of technology

The preparation of cerium oxide micro-nano particle matrix with low cost and simple steps is realized, which reduces background interference and hot spot effects in small molecule segments, improves detection sensitivity and flux, and meets the need to obtain clinical metabolic fingerprint maps.

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Abstract

The present invention discloses a method for preparing cerium oxide micro-nano particles with a multi-level structure, comprising the following steps: Step 1, preparation of a template of 3-aminophenol-formaldehyde spheres; the extended #imgabs0# method is applicable to the preparation of spherical APF resins with uniform particle sizes; Step 2, growing cerium oxide on APF to obtain APF-CeO2; Step 3, removing the APF template to obtain multi-level structured cerium oxide micro-nano particles. The present invention also provides the application of the above-mentioned cerium oxide micro-nano particles in the detection and identification of small molecule metabolites. The detection method of the present invention is non-invasive, has a high detection throughput, high sensitivity, and low cost, meets the clinical requirements for obtaining metabolic fingerprint maps, realizes clinical applications such as diagnosis, prognosis, and biomarker screening, and has great application potential in the in-depth interpretation of the processes of organism growth and development and pathogenic mechanisms at the metabolomics level.
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