A Chinese veterinary oral preparation for treating liver diseases and its preparation method and application

Abstract

The invention discloses a Chinese veterinary oral preparation for treating liver diseases for animals, and a preparation method and application thereof, and belongs to the technical field of veterinary medicine. The raw materials prepared by the Chinese veterinary oral preparation include the following components in parts by weight: 20-30 parts of turmeric, 25-35 parts of astragalus, 1-10 parts of isatis root, 5-15 parts of dodder seed, 1-3 parts of atractylodes macrocephala, and 1-2 parts of white peony root. The Chinese veterinary oral preparation for treating liver diseases for animals of the present invention uses turmeric as the monarch, astragalus as the minister, isatis root and dodder seed as the assistant, and atractylodes macrocephala and white peony root as the messenger, which can soothe the liver and gallbladder, nourish the liver and kidney, and promote blood circulation and remove blood stasis, and can well improve the egg production rate of laying hens and improve the eggshell quality.

Description

Technical Field

[0001] The invention belongs to the technical field of veterinary medicines, and particularly relates to an oral Chinese veterinary medicine preparation for treating liver diseases and a preparation method and application thereof. Background Art

[0002] With the rapid development and improvement of my country's animal husbandry, and the further improvement of people's living and cultural standards, the issue of animal-derived food safety has increasingly become the focus of people's close attention. With the implementation of the relevant national policies on "antibiotic reduction" and "antibiotic substitution", how to ensure the safety and quality of animal-derived products for livestock and poultry, and reduce the side effects and residues of antibiotics in livestock and poultry are the current problems that the livestock and poultry breeding industry urgently needs to solve. Due to its advantages such as safety, effectiveness and few side effects, Chinese veterinary medicine products can effectively solve the above problems and make great contributions to the development of livestock and poultry breeding in my country. The advantages of Chinese veterinary medicine in the process of use have gradually been highly valued by Chinese veterinary medicine R&D technicians and Chinese veterinary medicine livestock and poultry producers and breeders. Among them, the proportion of Chinese veterinary medicine R&D technology production and livestock and poultry breeding has also further expanded year by year. The industrial output value of Chinese veterinary medicine has increased significantly, and is gradually moving towards the industry goal of 10 billion yuan.

[0003] Poor egg-laying performance of laying hens is an important factor that seriously harms the laying hen farming industry. It can not only lead to low production performance and reduced quality of poultry products, but also affect people's economic income and foreign trade. In recent years, with the continuous increase in the number of laying hens in my country, there are many factors that affect the production performance of laying hens. For example, the long-term use of hormone drugs to regulate the production performance of laying hens can easily cause the body to develop drug resistance, and there are problems such as residual and toxic side effects. Summary of the invention

[0004] In view of the above problems, the present invention attempts to screen compound drug combinations from the perspectives of soothing the liver and promoting gallbladder function, nourishing the liver and kidney, and promoting blood circulation and removing blood stasis, so as to promote the nutrient metabolism and biotransformation of the laying hen's liver, thereby achieving the purpose of increasing the egg production rate and improving the eggshell quality. The Chinese veterinary oral preparation of the present invention uses Curcuma as the main ingredient, Astragalus as the minister, Radix Isatidis and Semen Cuscutae as the assistant ingredients, and Atractylodes macrocephala and Paeonia lactiflora as the messenger, which cooperate to play the effects of replenishing qi and nourishing blood, regulating the liver and kidney, promoting blood circulation and removing blood stasis, and clearing away heat and nourishing yin.

[0005] The first aspect of the present invention is to disclose a Chinese veterinary oral preparation for treating liver diseases, the raw materials for preparation thereof include the following components in parts by weight:

[0006] 20-30 parts of Curcuma, 25-35 parts of Astragalus, 1-10 parts of Isatis Root, 5-15 parts of Cuscuta Seed, 1-3 parts of Atractylodes, and 1-2 parts of White Peony Root.

[0007] In some embodiments of the present invention, in the prepared raw materials, the weight ratio of Curcuma, Astragalus, Isatis, Cuscuta, Atractylodes and White Peony is 1: (1-1.5): (0.1-0.4): (0.1-0.5): (0.05-0.1): (0.01-0.05).

[0008] In some embodiments of the present invention, the weight ratio of Curcuma, Astragalus, Isatis, Cuscuta, Atractylodes and White Peony is 1: (1.1-1.3): (0.1-0.3): (0.3-0.5): (0.06-0.1): (0.02-0.05).

[0009] In some embodiments of the present invention, the prepared raw materials include the following components in parts by weight:

[0010] 25 parts of Curcuma, 30 parts of Astragalus, 5 parts of Isatis Root, 10 parts of Cuscuta Seed, 2 parts of Atractylodes, and 1 part of White Peony Root.

[0011] In some embodiments of the present invention, the prepared raw materials also include oral preparation excipients.

[0012] In some embodiments of the present invention, the oral formulation excipients include deionized water and a preservative.

[0013] In some embodiments of the present invention, the extracts are obtained by extracting the turmeric, astragalus, isatis root, dodder seed, atractylodes and white peony root with a polar solvent, such as water, ethanol or an aqueous solution thereof.

[0014] In some embodiments of the present invention, the polar solvent extracts of Curcuma, Astragalus, Radix Isatidis, Cuscuta, Atractylodes and Paeonia lactiflora are stored in the form of raw materials such as extraction solution, diluent, concentrated solution or dry powder.

[0015] The second aspect of the present invention is to disclose a method for preparing the oral Chinese veterinary medicine preparation for treating liver diseases according to the first aspect, comprising the following steps:

[0016] S01, taking various Chinese herbal medicine raw materials, crushing them to obtain extraction raw materials;

[0017] S02, extracting the extraction raw material, wherein the extraction solvent is ethanol aqueous solution;

[0018] S03, filtering to obtain an extract;

[0019] S04, adding auxiliary materials and preparing to obtain the Chinese veterinary oral preparation for treating liver diseases.

[0020] In some embodiments of the present invention, in S02, the extraction solvent is 8-10 times the volume of 60-80% (v / v) ethanol aqueous solution.

[0021] In some embodiments of the present invention, in S02, extraction is performed at 50-75°C for 2-5 hours, preferably 2-3 times.

[0022] In some embodiments of the present invention, during the extraction of the raw material, mechanical stirring is used to improve the extraction effect and reduce the problem of uneven distribution of local materials. A fuzzy control algorithm is used to process the temperature obtained in real time by the temperature sensor in the stirring container to dynamically adjust the stirring speed, and the steps are as follows:

[0023] (1) Define the input variables reaction temperature (T), reaction temperature change rate (dT / dt) and output variable stirring speed (v), read the temperature and temperature change rate, and input them into the fuzzy controller;

[0024] (2) Initialize the fuzzy controller, calculate the weight value of each rule and the membership function of the output variable according to the membership function of the input variable and the set rule base, perform conjunction operation on the weight values ​​and membership functions of all rules to obtain the output membership function; calculate the center of gravity of the output membership function to obtain the fuzzy output value.

[0025] (3) Through defuzzification conversion, a specific and feasible output stirring speed value v is obtained, and the obtained stirring speed signal is output to the stirrer controller to achieve temperature control.

[0026] (4) Return to (1) and continue to wait for the next measured value reaction temperature f and reaction temperature change rate Δ f .

[0027] It is controlled by self-correcting fuzzy control using the following formula:

[0028]

[0029] Among them, β is the adjustment factor of self-correcting fuzzy control, which takes the value of [0, 1] and is related to f, Δ f related; L is the fuzzy control step size, Mε and M1 are the upper and lower thresholds of temperature deviation, f(n) and f(n-1) are the deviations of time n and n-1 respectively, f(ΔT c , S, α) is the control correction, ΔT c is the deviation between the target temperature and the actual temperature, S is the heating power, and α is the mass transfer coefficient.

[0030] The third aspect of the present invention is to disclose the use of the Chinese veterinary oral preparation for treating liver diseases described in the first aspect in improving the egg production rate of laying hens and improving the quality of eggshells.

[0031] Beneficial effects of the present invention:

[0032] The veterinary oral preparation for treating liver diseases of the present invention uses Curcuma as the main ingredient, Astragalus as the secondary ingredient, Radix Isatidis and Semen Cuscutae as the auxiliary ingredients, and Atractylodes macrocephala and Paeonia lactiflora as the envoys. It can soothe the liver and promote bile secretion, nourish the liver and kidney, and promote blood circulation and remove blood stasis, and can well improve the egg production rate of laying hens and improve the eggshell quality. DETAILED DESCRIPTION

[0033] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0034] Unless otherwise specified, the examples and comparative examples are parallel tests with the same components, component contents, preparation steps and preparation parameters.

[0035] Example 1

[0036] Preparation method of a Chinese veterinary oral preparation for treating liver diseases

[0037] (1) Take 20 parts of Curcuma, 25 parts of Astragalus, 3 parts of Radix Isatidis, 5 parts of Cuscuta, 1 part of Atractylodes macrocephala, and 1 part of White Peony Root, grind and mix them to obtain an extraction material;

[0038] (2) adding 5 times the volume of 70% ethanol aqueous solution, stirring, extracting at 65° C. for 1 h, filtering, adding 5 times the volume of 70% ethanol aqueous solution again, stirring, extracting at 65° C. for 2 h, filtering, and combining the filtrates of the two extractions;

[0039] (3) Add 0.1% (w / v) of the preservative benzoic acid to obtain the Chinese veterinary oral preparation for treating liver diseases.

[0040] Example 2

[0041] A method for preparing a Chinese veterinary oral preparation for treating liver diseases, which differs from Example 1 in that:

[0042] The Chinese herbal medicine raw materials used in the preparation are: 30 parts of Curcuma, 35 parts of Astragalus, 10 parts of Isatis Root, 15 parts of Cuscuta Seed, 3 parts of Atractylodes, and 2 parts of White Peony Root.

[0043] Example 3

[0044] A method for preparing a Chinese veterinary oral preparation for treating liver diseases, which differs from Example 1 in that:

[0045] The Chinese herbal medicine raw materials used in the preparation are: 25 parts of Curcuma, 30 parts of Astragalus, 5 parts of Isatis Root, 10 parts of Cuscuta Seed, 2 parts of Atractylodes Macrocephala, and 1 part of White Peony Root.

[0046] Example 4

[0047] A method for preparing an oral Chinese veterinary medicine for treating liver diseases is different from Example 1 in that: during the extraction of the extraction raw material, mechanical stirring is used to improve the extraction effect and reduce the problem of uneven distribution of local materials. A fuzzy control algorithm is used to process the temperature obtained in real time by the temperature sensor in the stirring container to dynamically adjust the stirring speed, and the steps are as follows:

[0048] (1) Define the input variables reaction temperature (T), reaction temperature change rate (dT / dt) and output variable stirring speed (v), read the temperature and temperature change rate, and input them into the fuzzy controller;

[0049] (2) Initialize the fuzzy controller, calculate the weight value of each rule and the membership function of the output variable according to the membership function of the input variable and the set rule base, perform conjunction operation on the weight values ​​and membership functions of all rules to obtain the output membership function; calculate the center of gravity of the output membership function to obtain the fuzzy output value.

[0050] (3) Through defuzzification conversion, a specific and feasible output stirring speed value v is obtained, and the obtained stirring speed signal is output to the stirrer controller to achieve temperature control.

[0051] (4) Return to (1) and continue to wait for the next measured value of reaction temperature and reaction temperature change rate.

[0052] It is controlled by self-correcting fuzzy control using the following formula:

[0053]

[0054] Among them, β is the adjustment factor of self-correcting fuzzy control, which takes the value of [0, 1] and is related to f, Δ f related; L is the fuzzy control step size, Mε and M1 are the upper and lower thresholds of temperature deviation, f(n) and f(n-1) are the deviations of time n and n-1 respectively, f(ΔT c , S, α) is the control correction, ΔT c is the deviation between the target temperature and the actual temperature, S is the heating power, and α is the mass transfer coefficient.

[0055] The results showed that for the powders of Curcuma, Astragalus, Radix Isatidis, Semen Cuscutae, Atractylodes macrocephala and Paeonia lactiflora below 300 mesh, in a three-paddle stirring system heated by a sandwich heating rod with an 8-fold volume of ethanol-water extraction solvent, at least three temperature sensors were set close to the measuring ring at the bottom of the reactor to obtain the temperature and temperature change rate. When the temperature difference between the two temperature sensors was greater than 0.2°C or the heating rate was greater than 5°C / min, the stirrer speed was increased by 5%. The abnormal deepening of the color of the extract and the generation of by-products can be significantly avoided.

[0056] Comparative Example 1

[0057] A method for preparing a Chinese veterinary oral preparation for treating liver diseases, which differs from Example 1 in that:

[0058] The Chinese herbal medicine raw materials used in the preparation are: 35 parts of Curcuma, 10 parts of Astragalus, 3 parts of Isatis, 5 parts of Cuscuta, 1 part of Atractylodes, and 1 part of White Peony.

[0059] Comparative Example 2

[0060] A method for preparing a Chinese veterinary oral preparation for treating liver diseases, which differs from Example 1 in that:

[0061] The Chinese herbal medicine raw materials used in the preparation are: 20 parts of Curcuma, 25 parts of Astragalus, 6 parts of Isatis Root, 1 part of Cuscuta Seed, 1 part of Atractylodes Macrocephala, and 1 part of White Peony Root.

[0062] Comparative Example 3

[0063] A method for preparing a Chinese veterinary oral preparation for treating liver diseases, which differs from Example 1 in that:

[0064] The Chinese herbal medicine raw materials used in the preparation are: 20 parts of Curcuma, 25 parts of Astragalus, 3 parts of Isatis Root, 5 parts of Cuscuta Seed, and 2 parts of Atractylodes.

[0065] Effects of experimental cases on egg production in laying hens

[0066] Test subjects: 48-week-old Jingbai No. 1 laying hens

[0067] Feeding and management: laying hens were allowed to eat and drink freely, and the chicken house was ventilated and supplemented with light. The control group was fed a basic diet, and the experimental group was fed a basic diet supplemented with the Chinese veterinary oral preparation of the embodiment and the comparative example (the volume can be appropriately concentrated) equivalent to 0.5 g / kg of the crude drug.

[0068] There were no less than 50 laying hens in each experimental group and control group.

[0069] The egg production and eggshell quality of laying hens after four weeks were counted, and the results are shown in Tables 1 and 2. Egg production rate = daily egg production / total laying hens × 100%, average egg weight = total egg weight / total number of eggs. Eggshell thickness The thickness of the tip, blunt end, and side end of the eggshell (without mucosa) was measured using a vernier caliper, and the average value was calculated. The eggshell strength was tested using an eggshell strength tester (eggshell strength tester ORKA).

[0070] Table 1 Egg laying performance

[0071]

[0072]

[0073] The study found that the egg production rate and average egg weight of Examples 1-3 were significantly better than those of Comparative Example 1, and Comparative Example 1 was significantly better than Comparative Examples 2, 3 and the blank control group. The results show that the Chinese herbal medicine components and content obtained in the oral Chinese veterinary preparation of the present invention significantly affect the egg-laying performance of laying hens.

[0074] Table 2 Eggshell quality

[0075] Eggshell thickness mm <![CDATA[Eggshell strength kg / m 2 > Example 1 0.34±0.02b 4.54±0.18b Example 2 0.34±0.01b 4.48±0.27b Example 3 0.34±0.01b 4.49±0.30b Comparative Example 1 0.32±0.02a 4.31±0.19a Comparative Example 2 0.32±0.01a 4.38±0.64a Comparative Example 3 0.32±0.01a 4.32±0.30a Control group (blank) 0.32±0.01a 4.37±0.58a

[0076] The study found that the eggshell thickness and eggshell strength of Examples 1-3 were significantly better than those of Comparative Examples 1-3 and the blank control group. The results show that the Chinese herbal medicine components and content obtained in the oral Chinese veterinary preparation of the present invention significantly affect the eggshell quality of laying hens.

[0077] The preferred specific implementation modes and embodiments of the present invention are described in detail above, but the present invention is not limited to the above implementation modes and embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the concept of the present invention.

Claims

1. A Chinese veterinary oral preparation for improving egg production rate of laying hens and eggshell quality, characterized in that: The raw materials are as follows by weight: 20-30 parts of Curcuma, 25-35 parts of Astragalus, 1-10 parts of Isatis Root, 5-15 parts of Cuscuta Seed, 1-3 parts of Atractylodes, and 1-2 parts of White Peony Root.

2. The Chinese veterinary oral preparation for improving the egg production rate of laying hens and the quality of eggshells according to claim 1, characterized in that: In the prepared raw materials, the weight ratio of Curcuma, Astragalus, Radix Isatidis, Cuscuta, Atractylodes and White Peony is 1: (1-1.5): (0.1-0.4): (0.1-0.5): (0.05-0.1): (0.01-0.05).

3. The Chinese veterinary oral preparation for improving the egg production rate of laying hens and the quality of eggshells according to claim 1 or 2, characterized in that: The weight ratio of Curcuma, Astragalus, Isatis Root, Cuscuta Seed, Atractylodes and White Peony Root is 1: (1.1-1.3): (0.1-0.3): (0.3-0.5): (0.06-0.1): (0.02-0.05).

4. The Chinese veterinary oral preparation for improving the egg production rate of laying hens and the quality of eggshells according to claim 1 or 2, characterized in that: The raw materials are as follows by weight: 25 parts of Curcuma, 30 parts of Astragalus, 5 parts of Isatis, 10 parts of Cuscuta, 2 parts of Atractylodes, and 1 part of White Peony Root.

5. The Chinese veterinary oral preparation for improving the egg production rate of laying hens and the quality of eggshells according to claim 1 or 2, characterized in that: The raw materials for preparation also include auxiliary materials for oral preparations.

6. The Chinese veterinary oral preparation for improving the egg production rate of laying hens and the quality of eggshells according to claim 5, characterized in that: The oral preparation excipients include deionized water and a preservative.

7. A method for preparing a Chinese veterinary oral preparation for improving the egg production rate of laying hens and the quality of eggshells according to any one of claims 1 to 6, characterized in that: The following steps are involved: S01, taking various Chinese herbal medicine raw materials, crushing them to obtain extraction raw materials; S02, extracting the extraction raw material, wherein the extraction solvent is ethanol aqueous solution; S03, filtering to obtain an extract; S04, adding auxiliary materials, preparing, and obtaining a Chinese veterinary medicine oral preparation.

8. The method for preparing the oral Chinese veterinary medicine preparation for improving the egg production rate of laying hens and the quality of eggshells according to claim 7, characterized in that: In S02, the extraction solvent is 8-10 times the volume of an ethanol aqueous solution with a volume percentage of 60-80%.

9. The method for preparing the oral Chinese veterinary medicine preparation for improving the egg production rate of laying hens and the quality of eggshells according to claim 7 or 8, characterized in that: In S02, extract at 50-75°C for 2-5h.

10. The method for preparing the oral Chinese veterinary medicine preparation for improving the egg production rate of laying hens and the quality of eggshells according to claim 9, characterized in that: In S02, extract at 50-75°C for 2-5h and extract 2-3 times.

11. Use of the oral Chinese veterinary preparation for improving the egg production rate and eggshell quality of laying hens according to any one of claims 1 to 6 in improving the egg production rate and eggshell quality of laying hens.

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