An immunochromatographic test strip using metal polyphenol network fluorescent microspheres as markers, and preparation method and application thereof
By using metal polyphenol network fluorescent microspheres (MPNFM) as a marker and using electrostatic adsorption of coupled antibodies, the problem of poor biocompatibility of traditional fluorescent microspheres is solved, and the sensitivity and stability of immunochromatography detection are improved.
CN118010985BActive Publication Date: 2025-04-22INSTITUTE OF MICROBIOLOGY JIANGXI ACADEMY OF SCIENCES (JIANGXI INSTITUTE OF WATERSHED ECOLOGY)
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Patent Information
- Application Number
- CN202410187562.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-02-20
- Publication Date
- 2025-04-22
- Estimated Expiration
- 2044-02-20
AI Technical Summary
Technical Problem
The poor biocompatibility of traditional fluorescent microspheres leads to low antibody coupling efficiency and insufficient sensitivity and stability in immunochromatography detection.
Method used
Metal polyphenol network fluorescent microspheres (MPNFM) were used as markers, and core-shell MPNFM was formed by mixing metal ions with aqueous polyphenol solution and adding them to fluorescent dye solution to react. This method improves the efficiency of antibody coupling by electrostatic adsorption.
Benefits of technology
It significantly improves the antibody coupling efficiency of fluorescent microspheres, enhances the sensitivity and stability of immunochromatography detection, and solves the problem of poor biocompatibility in traditional methods.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention discloses an immunochromatographic test strip with metal polyphenol network fluorescent microspheres as a marker, and a preparation method and application thereof. The method uses MPNFM as a marker material, not only has a fast labeling speed, but also has a high protein coupling rate, which is very beneficial to improving the detection sensitivity of the immunochromatographic method. By overlapping and pasting a sample pad, a glass fiber pad sprayed with an MPNFM test object antibody probe, a nitrocellulose membrane sprayed with an artificial coupled antigen of the test object and sprayed with anti-mouse antibody or anti-rabbit antibody as a quality control line, and absorbent paper on a bottom plate in sequence, and then cutting it into a 4 mm wide test strip with a strip cutter, it can be used for actual sample detection. Compared with the traditional immunochromatographic detection method using colloidal gold as a marker, this method has higher qualitative and quantitative sensitivity and is more stable.
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