Fluorescence detection device and microfluidic chip detection system

Abstract

The present application relates to a kind of fluorescence detection device and microfluidic chip detection system.The fluorescence detection device includes: light source providing device, configured to provide full spectrum light source;At least two optical channel boxes, configured to alternatively receive the full spectrum light source provided by light source providing device, and generate and send excitation light source;Optical channel box is also configured to receive the fluorescence generated by excitation light source excitation, and complete fluorescence detection;And switching mechanism, configured to make at least two optical channel boxes movement, to make one of at least two optical channel boxes receive the full spectrum light source provided by light source providing device.Switching mechanism makes at least two optical channel boxes movement, to make one of at least two optical channel boxes receive the full spectrum light source provided by light source providing device, convenient to operate, can realize the switching of multiple fluorescence detection channels, complete the fluorescence detection of multiple fluorescence detection channels, meet the demand of the detection target quantity required by different diseases.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fluorescence detection technology, and in particular to a fluorescence detection device and a microfluidic chip detection system. BACKGROUND

[0002] Nucleic acid detection is a molecular diagnostic technique that makes relevant diagnosis by detecting the structure or expression level of genetic material. It has very wide applications in molecular biology, forensic science, clinical diagnostics and biomedical science. Due to the low initial sample concentration, nucleic acid detection usually needs to be preceded by nucleic acid amplification. Real-time quantitative PCR is one of the most important technologies that can exponentially amplify specific DNA fragments. Real-time quantitative PCR is widely used in nucleic acid screening and diagnosis of these diseases due to its advantages of accuracy, sensitivity, etc.

[0003] Real-time fluorescence quantitative PCR is crucial for clinical applications. Simple and rapid PCR strategies cannot provide reliable nucleic acid quantitative results and gene copy number differences, which are closely related to the evaluation of patient conditions, monitoring of disease processes and evaluation of prognosis. And because different diseases require different amounts of detection targets, single detection channels lack detection scalability. In some related technologies, PCR instruments detect by setting multiple fluorescence detection channels, but the detection channel switching operation is complex, the device is large in size, the structure is complex, the cost is high, professional operators and professional environments are needed. SUMMARY

[0004] Some embodiments of the present application propose a fluorescence detection device and a microfluidic chip detection system to alleviate the problem of complex multi-channel fluorescence detection operation.

[0005] In one aspect of the present application, a fluorescence detection device is provided, comprising:

[0006] A light source providing device configured to provide a full-spectrum light source;

[0007] At least two optical channel boxes configured to selectively receive the full-spectrum light source provided by the light source providing device, and generate and emit excitation light sources; the optical channel boxes are further configured to receive fluorescence generated by the excitation light sources, and complete fluorescence detection; and

[0008] A switching mechanism configured to move the at least two optical channel boxes so that one of the at least two optical channel boxes receives the full spectrum light source provided by the light source providing device.

[0009] In some embodiments, the fluorescence detection device further comprises:

[0010] A temperature control device comprising a receiving portion for mounting the chip, the temperature control device being configured to control the temperature in the reaction cavity on the chip; and

[0011] An optical transmission pipeline connecting the optical channel box and the temperature control device, the optical transmission pipeline being configured to transmit the excitation light source to the reaction cavity and transmit the fluorescence generated by the excitation light source to the optical channel box.

[0012] In some embodiments, the temperature control device comprises:

[0013] A first heating member configured to heat the reaction cavity of the chip; and

[0014] A first heat conducting member disposed between the first heating member and the receiving portion, the first heat conducting member comprising a side surface opposite to the port of the optical transmission pipeline, the side surface being provided with a reflective curved surface, the reflective curved surface being configured to reflect the excitation light source passing through the reaction cavity to the reaction cavity.

[0015] In some embodiments, the temperature control device further comprises:

[0016] A second heating member disposed on a side of the receiving portion opposite to the first heat conducting member, the second heating member being configured to heat the reaction cavity of the chip, the second heating member being provided with a through hole allowing the optical transmission pipeline to pass through; and

[0017] A second heat conducting member disposed between the receiving portion and the second heating member, the second heat conducting member being provided with a through hole allowing the optical transmission pipeline to pass through.

[0018] In some embodiments, the light source providing device comprises:

[0019] A first light transmission hole configured to transmit the full spectrum light source; and

[0020] At least two light source providing members configured to provide the full spectrum light source to the first light transmission hole at least alternatively.

[0021] In some embodiments, the at least two light source providing members include a first light source providing member and a second light source providing member, the first light source providing member emits full spectrum light source in a transmission direction consistent with the extension direction of the first light passing hole, the second light source providing member emits full spectrum light source in a transmission direction having an included angle greater than zero with the extension direction of the first light passing hole; the light source providing device further includes a first beam splitter, the first beam splitter is obliquely arranged in the first light passing hole, the first beam splitter is configured to allow the full spectrum light source provided by the first light source providing member to pass through, and is configured to reflect the full spectrum light source provided by the second light source providing member, so that the transmission direction is consistent with the extension direction of the first light passing hole.

[0022] In some embodiments, the light source providing device further includes at least two first converging lenses, the at least two first converging lenses are respectively and one by one arranged at the emission end of the at least two light source providing members, the first converging lens is configured to converge the full spectrum light source provided by the light source providing member and then transmit it to the first light passing hole.

[0023] In some embodiments, the optical channel box includes:

[0024] a fluorescence detection member configured to perform fluorescence detection;

[0025] a second light passing hole configured to communicate with the first light passing hole;

[0026] a third light passing hole in communication with the second light passing hole, the third light passing hole is configured to transmit excitation light source to the outside of the optical channel box, and receive fluorescence excited by the excitation light source returned from the outside of the optical channel box; and

[0027] a fourth light passing hole in communication with the third light passing hole, the fourth light passing hole is configured to guide fluorescence to the fluorescence detection member.

[0028] In some embodiments, the communication part of the third light passing hole respectively communicates with the second light passing hole and the fourth light passing hole, the optical channel box further includes a second beam splitter, the second beam splitter is obliquely arranged in the communication part, the second beam splitter is configured to make the excitation light source transmitted by the second light passing hole enter the third light passing hole, and make the fluorescence transmitted by the third light passing hole enter the fourth light passing hole.

[0029] In some embodiments, the third light passing hole and the fourth light passing hole are located in the same extension direction, and the extension direction of the second light passing hole and the extension direction of the third light passing hole have an included angle greater than zero.

[0030] In some embodiments, the optical channel box further includes:

[0031] a first filter disposed in the second light transmission hole; and

[0032] a second filter disposed in the fourth light transmission hole.

[0033] In some embodiments, the optical channel cartridge further comprises:

[0034] a second converging lens disposed in the third light transmission hole; and / or

[0035] a third converging lens disposed in the fourth light transmission hole.

[0036] In some embodiments, the number of the second filters is two, and the third converging lens is disposed between the two second filters.

[0037] In some embodiments, the fluorescence detection device further comprises a light transmission pipeline configured to communicate with the third light transmission hole to transmit the excitation light source provided by the optical channel cartridge or to transmit the fluorescence excited by the excitation light source into the optical channel cartridge.

[0038] In some embodiments, the at least two optical channel cartridges are arranged around a rotation axis.

[0039] In some embodiments, the light source providing device comprises a first light transmission hole transmitting a full spectrum light source, the optical channel cartridge comprises a second light transmission hole and a third light transmission hole, the second light transmission hole is disposed at a side of the optical channel cartridge, the second light transmission hole is configured to communicate with the first light transmission hole, the third light transmission hole is disposed at a top of the optical channel cartridge, and the third light transmission hole is configured to transmit the excitation light source outwardly or to transmit the fluorescence excited by the excitation light source inwardly.

[0040] In some embodiments, the switching mechanism comprises a carrier and a rotating shaft, the rotating shaft is disposed in the carrier and configured to drive the carrier to rotate, and the at least two optical channel cartridges are disposed in the carrier and arranged around the rotating shaft.

[0041] In some embodiments, the switching mechanism further comprises:

[0042] a power member;

[0043] a chassis, the power member and the carrier are disposed above the chassis;

[0044] a first wheel and a second wheel disposed below the chassis, the first wheel is connected to the power member, and the second wheel is connected to the rotating shaft; and

[0045] a belt connecting the first wheel and the second wheel.

[0046] In another aspect of the present application, a microfluidic chip detection system is provided, comprising a chip and the fluorescence detection device as described above.

[0047] Based on the above technical solution, the present application has at least the following beneficial effects:

[0048] In some embodiments, the light source providing device provides full spectrum excitation light source which is collimated and incident into the optical channel box in parallel; the optical channel box comprises corresponding optical elements for realizing accurate control of excitation light source and fluorescence emission; the switching mechanism moves at least two optical channel boxes so that one of the at least two optical channel boxes receives the full spectrum light source provided by the light source providing device, which is convenient to operate and can realize switching of multiple fluorescence detection channels and complete fluorescence detection of the multiple fluorescence detection channels, thereby meeting the requirement of different diseases for the number of detection targets. BRIEF DESCRIPTION OF DRAWINGS

[0049] The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiments of the application and serve to explain the principles of the application, and do not limit the application. In the drawings:

[0050] Figure 1 A schematic view of the fluorescence detection device according to some embodiments of the present application;

[0051] Figure 2 An exploded schematic view of the fluorescence detection device according to some embodiments of the present application;

[0052] Figure 3 A schematic view of the structure of the fluorescence detection device after the shell is opened according to some embodiments of the present application;

[0053] Figure 4 A top view schematic view of the fluorescence detection device after the shell is removed according to some embodiments of the present application;

[0054] Figure 5 An exploded schematic view of the temperature control device according to some embodiments of the present application;

[0055] Figure 6 A schematic view of the side surface of the first heat conducting member provided with a reflecting curved surface according to some embodiments of the present application;

[0056] Figure 7 A schematic view of the internal structure of the light source providing device according to some embodiments of the present application;

[0057] Figure 8a A schematic view of the internal structure of the optical channel box according to some embodiments of the present application;

[0058] Figure 8bFig. 1 is a schematic view of the internal components of an optical channel box according to some embodiments of the present application;

[0059] Figure 9 Fig. 2 is an exploded structural schematic view of a chip according to some embodiments of the present application;

[0060] Figure 10 Fig. 3 is a schematic view of a light source providing device providing a light source according to some embodiments of the present application;

[0061] Figure 11 Fig. 4 is a schematic view of an optical channel box according to some embodiments of the present application performing light source transmission;

[0062] Figures 12a to 12d Fig. 5 is a schematic view of four reflective curved surfaces provided on the side of a first heat conducting member according to some embodiments of the present application performing light source reflection.

[0063] The reference signs in the drawings are explained as follows:

[0064] 1 - light source providing device; 11 - first light transmission hole; 12 - light source providing member; 121 - first light source providing member; 122 - second light source providing member; 123 - third light source providing member; 13 - first beam splitter; 14 - first converging lens; 15 - third beam splitter;

[0065] 2 - optical channel box; 21 - fluorescence detection member; 22 - second light transmission hole; 23 - third light transmission hole; 24 - fourth light transmission hole; 25 - second beam splitter; 26 - first filter; 27 - second filter; 28 - second converging lens; 29 - third converging lens;

[0066] 3 - switching mechanism; 31 - bearing member; 32 - rotating shaft; 33 - power member; 34 - base frame; 35 - first wheel; 36 - second wheel; 37 - belt; 38 - top plate; 39 - housing; 391 - outer cover; 392 - bottom shell; 393 - top through hole; 394 - avoiding slot;

[0067] 4 - temperature control device; 41 - first heating member; 42 - second heating member; 43 - first heat conducting member; 431 - side surface; 4311 - reflective curved surface; 44 - second heat conducting member; 45 - first heat insulation member; 46 - second heat insulation member; 47 - fixing member;

[0068] 5 - light transmission pipeline;

[0069] 6 - chip; 61 - reaction cavity; 62 - base plate; 63 - cover plate; 64 - sample inlet; 65 - exhaust port; 66 - liquid inlet channel; 67 - exhaust channel; 68 - first plug; 69 - second plug.

[0070] It should be understood that the dimensions of the various parts shown in the drawings are not necessarily to scale. Also, like reference numerals are used to indicate like parts throughout the several views. DETAILED DESCRIPTION

[0071] Various exemplary embodiments of the present application will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is only meant to be illustrative and not limiting of the present application. The present application can be carried out in many different ways with many different embodiments, not just the ones described here. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the application to those skilled in the art. It should be noted that the relative arrangement of components and steps, the numerical expressions, and the numerical values set forth in these embodiments are to be interpreted as merely exemplary and not limiting.

[0072] The terms "first", "second", and similar terms in the present application do not denote any order, quantity, or importance, but are used to distinguish different parts. The terms "include", "comprise", and similar terms mean that the elements before the term encompass the elements listed after the term, and do not exclude the possibility of also encompassing other elements. The terms "upper", "lower", "left", "right", and the like are used only to indicate relative positional relationships, and when the absolute positions of the described objects are changed, the relative positional relationships can also be changed accordingly.

[0073] In the present application, when it is described that a particular device is located between a first device and a second device, there can be an intervening device between the particular device and the first device or the second device, or there can be no intervening device. When it is described that a particular device is connected to other devices, the particular device can be directly connected to the other devices without an intervening device, or can not be directly connected to the other devices with an intervening device.

[0074] All terms used in the present application, including technical or scientific terms, have the same meanings as those generally understood by those skilled in the art to which the present application pertains, unless otherwise defined in the present application. It should also be understood that the terms, such as those defined in a generally used dictionary, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant technology and not in an idealized or overly formal sense unless explicitly so defined herein.

[0075] Techniques, methods, and devices known to those of ordinary skill in the relevant art can not be discussed in detail herein, but should be considered as if the discussion were in place for the sake of brevity.

[0076] Figures 1 to 3 is a structural schematic diagram of some embodiments of the fluorescence detection device according to the present application. Referring to Figures 1 to 3In some embodiments, the fluorescence detection device comprises a light source providing apparatus 1, at least two optical channel boxes 2, and a switching mechanism 3.

[0077] The light source providing apparatus 1 is configured to provide a full-spectrum light source.

[0078] The at least two optical channel boxes 2 are configured to receive the full-spectrum light source provided by the light source providing apparatus 1 alternatively, and generate and emit an excitation light source; the optical channel boxes 2 are further configured to receive the fluorescence generated by the excitation light source, and complete fluorescence detection.

[0079] The switching mechanism 3 is configured to move the at least two optical channel boxes 2, so that one of the at least two optical channel boxes 2 receives the full-spectrum light source provided by the light source providing apparatus 1.

[0080] The light source providing apparatus 1 can provide a full-spectrum excitation light source, which is collimated and incident on the optical channel boxes 2 in parallel. The optical channel boxes 2 include corresponding optical elements therein, for realizing precise control of the excitation light source and the emitted fluorescence. The switching mechanism 3 moves the at least two optical channel boxes 2, so that one of the at least two optical channel boxes 2 receives the full-spectrum light source provided by the light source providing apparatus 1, which is convenient to operate, can complete switching of at least two fluorescence detection channels, complete fluorescence detection of multiple fluorescence detection channels, and meet the requirement of the number of detection targets required by different diseases.

[0081] In some embodiments, the at least two optical channel boxes 2 can include two optical channel boxes 2, three optical channel boxes 2, four optical channel boxes 2, five optical channel boxes 2, six optical channel boxes 2, or more than six optical channel boxes 2, which can complete multi-channel fluorescence detection and have great detection potential.

[0082] Reference Figures 1 to 4 In some embodiments, the fluorescence detection device further comprises a temperature control apparatus 4. The temperature control apparatus 4 comprises a containing portion for mounting a microfluidic chip 6, and the temperature control apparatus 4 is configured to control the temperature in a reaction cavity 61 on the chip 6. Optionally, the reaction cavity 61 comprises an amplification cavity for performing a nucleic acid amplification reaction for nucleic acid detection.

[0083] Reference Figures 1 to 4 In some embodiments, the fluorescence detection device further comprises a light transmission pipeline 5. The light transmission pipeline 5 connects the optical channel boxes 2 and the temperature control apparatus 4, and the light transmission pipeline 5 is configured to transmit the excitation light source to the reaction cavity 61, and transmit the fluorescence generated by the excitation light source in the reaction cavity 61 to the optical channel boxes 2.

[0084] In the above embodiments, by providing the temperature control apparatus 4, real-time multi-channel fluorescence detection can be completed under the limitation of ultra-fast temperature rising and falling conditions for optical detection.

[0085] In some embodiments, the light transmission pipeline 5 comprises an optical fiber.

[0086] With reference to Figure 5 In some embodiments, the temperature control device 4 comprises a first heating member 41 and a first heat conduction member 43.

[0087] The first heating member 41 is configured to heat the reaction cavity 61 of the chip 6.

[0088] The first heat conduction member 43 is arranged between the first heating member 41 and the accommodating portion.

[0089] With reference to Figure 6 The first heat conduction member 43 comprises a side surface 431 arranged opposite to the port of the light transmission pipeline 5 towards the reaction cavity 61, and a reflective curved surface 4311 arranged on the side surface 431, the reflective curved surface 4311 being configured to reflect the excitation light source passing through the reaction cavity 61 to the reaction cavity 61.

[0090] The light transmission pipeline 5 connects the optical channel box 2 and the temperature control device 4, and transmits the excitation light source to the reaction cavity 61, part of the excitation light source passes through the reaction cavity 61 and is incident on the first heat conduction member 43, the reflective curved surface 4311 on the first heat conduction member 43 reflects the excitation light source back to the reaction cavity 61, which can uniformly reflect the excitation light source on the reaction solution in the reaction cavity 61 without loss of temperature, and re-excite the fluorescent substance in the reaction cavity 61, thereby improving the fluorescent excitation efficiency and signal-to-noise ratio.

[0091] In some embodiments, through precise control of the light path and in combination with the special optical reflective curved surface 4311 arranged on the first heat conduction member 43, efficient and sensitive multi-channel real-time fluorescence detection can be achieved, which realizes more efficient fluorescence excitation and real-time fluorescence collection, and greatly improves the system detection performance.

[0092] In the embodiments of the present application, the fluorescence collection part is integrated into the temperature control part for optical design, and an optical reflective curved surface is designed on the first heat conduction member 43, which improves the overall performance of optical detection without affecting the rapid change of temperature.

[0093] In some embodiments, the temperature control device 4 further comprises a second heating member 42 and a second heat conduction member 44.

[0094] The second heating member 42 is arranged on the side of the accommodating portion opposite to the first heat conduction member 43, and the second heating member 42 is configured to heat the reaction cavity 61 of the chip 6, and the second heating member 42 is provided with a through hole allowing the light transmission pipeline 5 to pass through.

[0095] The second heat conduction member 44 is arranged between the accommodating portion and the second heating member 42, and the second heat conduction member 44 is provided with a through hole allowing the light transmission pipeline 5 to pass through.

[0096] In the above embodiment, the temperature control device 4 as a whole presents a "sandwich" heating structure clamping the chip 6 from top to bottom, the optical reflection curved surface 4311 is arranged on the first heat conduction member 43, so that the excitation light source can uniformly irradiate the reaction solution in the reaction cavity 61 without temperature loss, the fluorescent substance in the reaction cavity 61 is excited again, and the fluorescent excitation efficiency and signal-to-noise ratio are improved.

[0097] In some embodiments, the chip 6 is a flat sheet.

[0098] In some embodiments, the first heat conduction member 43 and the second heat conduction member 44 are both flat, the first heat conduction member 43 includes a side surface 431 abutting against the chip 6, the second heat conduction member 44 includes a side surface abutting against the chip 6, the first heat conduction member 43 is provided with a reflection curved surface 4311 on the side surface 431 abutting against the chip 6, and the position of the reflection curved surface 4311 corresponds to the position of the reaction cavity 61 on the chip 6.

[0099] In some embodiments, the side surface of the first heat conduction member 43 away from the chip 6 is provided with a containing groove containing the first heating member 41. The side surface of the second heat conduction member 44 away from the chip 6 is provided with a containing groove containing the second heating member 42.

[0100] In some embodiments, the temperature control device 4 further includes a first heat insulation member 45 arranged on the side of the first heating member 41 away from the first heat conduction member 43. The temperature control device 4 further includes a second heat insulation member 46 arranged on the side of the second heating member 42 away from the second heat conduction member 44.

[0101] In some embodiments, the first heating member 41 is located between the first heat insulation member 45 and the first heat conduction member 43, the second heating member 42 is located between the second heat insulation member 47 and the second heat conduction member 44, and the first heat conduction member 43 and the second heat conduction member 44 have a containing portion containing the chip 6. The chip 6 is arranged in the containing portion between the first heat conduction member 43 and the second heat conduction member 44.

[0102] Since the chip 6 is a flat sheet, the first heating member 41 and the second heating member 42 are configured as sheets, and the first heat conduction member 43 and the second heat conduction member 44 are both configured as flat, so that the reaction cavity 61 of the chip 6 can be rapidly changed in temperature.

[0103] In some embodiments, the first heat insulation member 45 and the second heat insulation member 46 are also configured as flat.

[0104] In some embodiments, the temperature control device 4 further includes a fixing member 47 configured to fix the light transmission pipeline 5. The light transmission pipeline 5 first passes through the fixing member 47, and then passes through the second heat insulation member 46, the second heating member 42 and the second heat conduction member 44 in sequence to align with the reaction cavity 61 on the chip 6.

[0105] In some embodiments, the first heat-conducting member 43 is made of aluminum material. The second heat-conducting member 44 is made of aluminum material.

[0106] With reference to Figure 7 In some embodiments, the light source providing device 1 comprises the first light transmission hole 11 and at least two light source providing members 12.

[0107] The first light transmission hole 11 is configured to transmit full-spectrum light source.

[0108] The at least two light source providing members 12 are configured to provide the full-spectrum light source to the first light transmission hole 11 at least alternatively.

[0109] During the detection process, at least one of the at least two light source providing members 12 provides the full-spectrum light source. The at least two light source providing members 12 can provide at least two colors of full-spectrum light source.

[0110] The light source providing device 1 integrates the at least two light source providing members 12, which can reduce the overall volume of the system.

[0111] The light source providing member 12 is used to provide full-spectrum excitation light source. In some embodiments, the light source providing member 12 comprises LED lamp, LD lamp or halogen lamp, etc.

[0112] In some embodiments, the at least two light source providing members 12 comprise a first light source providing member 121 and a second light source providing member 122, the first light source providing member 121 emits full-spectrum light source in the same direction as the extension direction of the first light transmission hole 11, and the second light source providing member 122 emits full-spectrum light source in the direction having an included angle greater than zero with the extension direction of the first light transmission hole 11; the light source providing device 1 further comprises a first beam splitter 13, which is obliquely arranged in the first light transmission hole 11, and the first beam splitter 13 is configured to allow the full-spectrum light source provided by the first light source providing member 121 to pass through, and the first beam splitter 13 is configured to reflect the full-spectrum light source provided by the second light source providing member 122, so that the transmission direction of the full-spectrum light source provided by the second light source providing member 122 is consistent with the extension direction of the first light transmission hole 11.

[0113] In some embodiments, the at least two light source providing members 12 further comprise a third light source providing member 123 and a third beam splitter 15, which is obliquely arranged in the first light transmission hole 11. In the transmission direction of the light source in the first light transmission hole 11, the third beam splitter 15 is located downstream of the first beam splitter 13, the third beam splitter 15 is configured to allow the full-spectrum light source provided by the first light source providing member 121 and the third light source providing member 123 to pass through, and the third beam splitter 15 is configured to reflect the full-spectrum light source provided by the third light source providing member 123, so that the transmission direction of the full-spectrum light source provided by the third light source providing member 123 is consistent with the extension direction of the first light transmission hole 11.

[0114] Optionally, the light source providing device 1 comprises three light source providing members 12, which are white light LED, blue light LED, and red light LED, respectively.

[0115] Optionally, the first beam splitter 13 and the third beam splitter 15 can both be dichroic mirrors.

[0116] In some embodiments, the first light transmission holes 11 are arranged in parallel, and the light source emitted by the first light source providing member 121 is transmitted in parallel into the first light transmission hole 11. The light source emitted by the second light source providing member 122 is reflected by the first beam splitter 13 and then transmitted in parallel into the first light transmission hole 11, and the light source of the third light source providing member 123 is reflected by the third beam splitter 15 and then transmitted in parallel into the first light transmission hole 11.

[0117] The main function of the light source providing device 1 is to complete the parallel emission of a specific excitation light waveband.

[0118] In some embodiments, the light source providing device 1 further comprises at least two first converging lenses 14, which are arranged one by one at the emission ends of the at least two light source providing members 12, respectively. The first converging lens 14 is configured to converge the full-spectrum light source provided by the light source providing member 12 and then transmit it to the first light transmission hole 11.

[0119] In some embodiments, the first converging lens 14 comprises a plano-convex lens.

[0120] In some embodiments, a first converging lens 14 is arranged between the first light source providing member 121 and the first beam splitter 13, another first converging lens 14 is arranged between the second light source providing member 122 and the first beam splitter 13, and a first converging lens 14 is further arranged between the third light source providing member 123 and the third beam splitter 15. Figure 7 In the embodiment of the light source providing device 1 shown in the figure, three first converging lenses 14 are arranged, that is, a first converging lens 14 is arranged at the emission end of each light source providing member 12.

[0121] In some embodiments, the light source provides that the device 1 in combination with the beam splitter and the converging lens can make the different waveband excitation light source controllable, and finally emit collimated parallel excitation light.

[0122] Reference Figure 8a and Figure 8b In some embodiments, the optical channel box 2 includes a fluorescence detection piece 21, a second light transmission hole 22, a third light transmission hole 23, and a fourth light transmission hole 24.

[0123] The fluorescence detection piece 21 is configured to perform fluorescence detection.

[0124] The second light transmission hole 22 is configured to communicate with the first light transmission hole 11.

[0125] The third light transmission hole 23 communicates with the second light transmission hole 22, and the third light transmission hole 23 is configured to transmit the excitation light source to the outside of the optical channel box 2 and receive the fluorescence excited by the excitation light source returned from the outside of the optical channel box 2.

[0126] The fourth light transmission hole 24 communicates with the third light transmission hole 23, and the fourth light transmission hole 24 is configured to guide the fluorescence to the fluorescence detection piece 21.

[0127] The main function of the optical channel box 2 is to realize the precise control of the excitation light and the emission fluorescence, specifically to converge the excitation light of the corresponding channel at the end of the light transmission pipeline 5, and to finally incident the fluorescence collected through the light transmission pipeline 5 to the fluorescence detection piece 21 through filtering processing for detection by the fluorescence detection piece 21.

[0128] In some embodiments, the fluorescence detection piece 21 includes a photoelectric detection element. Optionally, the photoelectric detection element includes a PMT (photomultiplier tube), a PD (photodiode), or an APD (avalanche photodiode), etc.

[0129] In some embodiments, the communication part of the third light transmission hole 23 respectively communicates with the second light transmission hole 22 and the fourth light transmission hole 24, and the optical channel box 2 further includes a second beam splitter 25, which is obliquely arranged at the communication part, and the second beam splitter 25 is configured to make the excitation light source transmitted by the second light transmission hole 22 enter the third light transmission hole 23, and make the fluorescence transmitted by the third light transmission hole 23 enter the fourth light transmission hole 24.

[0130] The second beam splitter 25 includes a dichroic mirror.

[0131] In some embodiments, the third light transmission hole 23 and the fourth light transmission hole 24 are located in the same extension direction, and the extension direction of the second light transmission hole 22 and the extension direction of the third light transmission hole 23 have an included angle greater than zero. Optionally, the extension direction of the second light transmission hole 22 and the extension direction of the third light transmission hole 23 have an included angle of 90 degrees.

[0132] The second beam splitter 25 is configured to reflect the excitation light source transmitted by the second light transmission hole 22, so that the transmission direction of the excitation light source transmitted by the second light transmission hole 22 is consistent with the extension direction of the third light transmission hole 23.

[0133] In some embodiments, the optical channel box 2 further comprises a first filter 26 and a second filter 27.

[0134] The first filter 26 is arranged at the second light transmission hole 22.

[0135] The second filter 27 is arranged at the fourth light transmission hole 24.

[0136] In some embodiments, the optical channel box 2 further comprises a second converging lens 28. The second converging lens 28 is arranged at the third light transmission hole 23. Optionally, the second converging lens 28 comprises a plane convex mirror.

[0137] The second converging lens 28 is configured to converge the excitation light source transmitted through the third light transmission hole 23. The second converging lens 28 is further configured to converge the reflected fluorescent light and transmit the reflected fluorescent light to the fourth light transmission hole 24 through the third light transmission hole 23.

[0138] In some embodiments, the optical channel box 2 further comprises a third converging lens 29. The third converging lens 29 is arranged at the fourth light transmission hole 24. Optionally, the third converging lens 29 comprises a plane convex mirror.

[0139] The third converging lens 29 is configured to converge the reflected fluorescent light and transmit the reflected fluorescent light to the fluorescent detection member 21.

[0140] In some embodiments, the number of the second filters 27 is two, and the third converging lens 29 is arranged between the two second filters 27.

[0141] In some embodiments, the fluorescent detection device further comprises a light transmission pipeline 5, which is configured to communicate with the third light transmission hole 23 to transmit the excitation light source provided by the optical channel box 2 or transmit the fluorescent light to the optical channel box 2.

[0142] In some embodiments, at least two optical channel boxes 2 are arranged around the rotation axis.

[0143] In some embodiments, referring to Figure 7 , the light source providing device 1 comprises a first light transmission hole 11 for transmitting a full-spectrum light source. Referring to Figures 2 to 4The optical channel box 2 comprises a second light transmission hole 22 and a third light transmission hole 23. The second light transmission hole 22 is arranged on the side of the optical channel box 2 and is configured to communicate with the first light transmission hole 11. The third light transmission hole 23 is arranged on the top of the optical channel box 2 and is configured to communicate with the light transmission pipeline 5 to transmit the excitation light source to the outside of the optical channel box 2 or transmit the fluorescent light generated by the excitation light source to the inside of the optical channel box 2.

[0144] With reference to Figure 2 In some embodiments, the switching mechanism 3 comprises a carrier 31 and a rotating shaft 32. The rotating shaft 32 is arranged through the carrier 31 and configured to drive the carrier 31 to rotate. The at least two optical channel boxes 2 are arranged on the carrier 31 and around the rotating shaft 32.

[0145] With reference to Figure 2 In some embodiments, the switching mechanism 3 further comprises a power member 33 configured to provide power for the rotation of the at least two optical channel boxes 2. Optionally, the power member 33 comprises an electric motor.

[0146] With reference to Figure 2 In some embodiments, the switching mechanism 3 further comprises a chassis 34. The power member 33 and the carrier 31 are arranged above the chassis 34. The arrangement of the power member 33 and the carrier 31 above the chassis 34 can reduce the overall axial height of the device and make rational use of the space on the chassis 34.

[0147] With reference to Figure 2 In some embodiments, the switching mechanism 3 further comprises a belt 37, a first wheel 35 and a second wheel 36. The first wheel 35 and the second wheel 36 are arranged below the chassis 34. The first wheel 35 is connected to the power member 33 and the second wheel 36 is connected to the rotating shaft 32. The belt 37 is connected to the first wheel 35 and the second wheel 36.

[0148] In some embodiments, the switching mechanism 3 further comprises a top plate 38. The at least two optical channel boxes 2 are arranged between the top plate 38 and the carrier 31 and connected by a connecting member. The top plate 38 is provided with through holes. The number of the through holes is the same as the number of the optical channel boxes 2. The third light transmission hole 23 of each optical channel box 2 is aligned with a through hole on the top plate 38. The top plate 38, the carrier 31 and the at least two optical channel boxes 2 arranged therebetween can rotate synchronously as a whole.

[0149] With reference to Figure 3 In some embodiments, the switching mechanism 3 further comprises a housing 39. The housing 39 comprises an outer shell 391 and a bottom shell 392. The bottom shell 392 is arranged on the chassis 34. The outer shell 391 is connected to the bottom shell 392 and used to cover the set assembly composed of the top plate 38, the carrier 31 and the at least two optical channel boxes 2.

[0150] The top of the shell 391 is provided with a top through hole 393 for allowing the light transmission pipe 5 to be inserted, and the side of the shell 39 is provided with a relief groove 394 for allowing the first light transmission hole 11 and the second light transmission hole 22 to communicate. When the assembled set composed of the top plate 38, the carrier 31 and the at least two optical channel boxes 2 is rotated, the second light transmission hole 22 of one of the optical channel boxes 2 is communicated with the first light transmission hole 11 through the relief groove 394 of the side of the shell 39, and the third light transmission hole 23 of the optical channel box 2 is communicated with the light transmission pipe 5 passing through the top through hole 393 of the shell 39.

[0151] Optionally, the shell 39 is a cylindrical shell.

[0152] The following will be described in detail with reference to the accompanying drawings Figures 1 to 1 2. Some specific embodiments of the fluorescence detection device provided by the present application are described in detail.

[0153] Reference Figures 1 to 4 The fluorescence detection device comprises a light source providing device 1, an optical channel box 2, a switching mechanism 3, a temperature control device 4 and a light transmission pipe 5.

[0154] Reference Figure 7 The light source providing device 1 comprises a first light transmission hole 11, a first light source providing part 121, a second light source providing part 122, a third light source providing part 123, a first beam splitter 13, a third beam splitter 15 and three first converging lenses 14.

[0155] The first light source providing member 121 is arranged at the first end of the first light transmission hole 11, and the light source emitted by the first light source providing member 121 is parallel to the extending direction of the first light transmission hole 11. The second light source providing member 122 is arranged between the first end and the second end of the first light transmission hole 11, and the light source emitted by the second light source providing member 122 is perpendicular to the extending direction of the first light transmission hole 11. The first beam splitter 13 is arranged in the first light transmission hole 11, and it allows the light source provided by the first light source providing member 121 to pass through, and reflects the light source provided by the second light source providing member 122 into the first light transmission hole 11, so that the transmission direction of the light source provided by the second light source providing member 122 is consistent with the extending direction of the first light transmission hole 11. The third light source providing member 123 is arranged between the first end and the second end of the first light transmission hole 11, and the third light source providing member 123 is closer to the second end of the first light transmission hole 11 than the second light source providing member 122. The light source emitted by the third light source providing member 123 is perpendicular to the extending direction of the first light transmission hole 11. The third beam splitter 15 is arranged in the first light transmission hole 11, and it allows the light source provided by the first light source providing member 121 and the second light source providing member 122 to pass through, and reflects the light source provided by the third light source providing member 123 into the first light transmission hole 11, so that the transmission direction of the light source provided by the third light source providing member 123 is consistent with the extending direction of the first light transmission hole 11. The second end of the first light transmission hole 11 is the light source transmission end. The first light source providing member 121, a first converging lens 14, the first beam splitter 13, and the third beam splitter 15 are arranged in the first light transmission hole 11 in sequence from the first end to the second end. A first converging lens 14 is arranged between the second light source providing member 122 and the first beam splitter 13. A first converging lens 14 is arranged between the third light source providing member 123 and the third beam splitter 15.

[0156] Optionally, the first light source providing member 121 is a blue LED lamp, the second light source providing member 122 is a white LED lamp, and the third light source providing member 123 is a red LED lamp. The first converging lens 14 is a plano-convex lens. The first beam splitter 13 and the third beam splitter 15 are both dichroic mirrors.

[0157] At least one of the first light source providing member 121, the second light source providing member 122, and the third light source providing member 123 provides a full-spectrum light source. The light source providing device 1 can provide a full-spectrum excitation light, which is collimated and parallel to the optical channel box 2. Figure 10 The schematic diagram of the simulation of the light source provided by the light source providing device 1 is shown in FIG. 2.

[0158] The schematic diagram of the simulation of the light source provided by the light source providing device 1 is shown in FIG. 2. Figure 8a and Figure 8bThe optical channel box 2 comprises a second light transmission hole 22, a third light transmission hole 23, a fourth light transmission hole 24, a fluorescence detection member 21, a first filter 26, a second beam splitter 25, a second converging lens 28, a third converging lens 29 and two second filters 27.

[0159] The first end of the third light transmission hole 23 is in communication with the second light transmission hole 22 and the fourth light transmission hole 24 respectively. The first end of the third light transmission hole 23 is a communication part. The second end of the third light transmission hole 23 is used for communication with the light transmission pipeline 5. The third light transmission hole 23 and the fourth light transmission hole 24 are located in the same extension direction, and the extension direction of the second light transmission hole 22 is perpendicular to the extension direction of the third light transmission hole 23. The second beam splitter 25 is inclinedly arranged at the communication part. The second beam splitter 25 is used for making the excitation light source transmitted by the second light transmission hole 22 enter the third light transmission hole 23, and making the fluorescence transmitted by the third light transmission hole 23 enter the fourth light transmission hole 24. The first filter 26 is arranged in the second light transmission hole 22. The second converging lens 28 is arranged in the third light transmission hole 23. The two second filters 27 and the third converging lens 29 are arranged in the fourth light transmission hole 24, and the third converging lens 29 is arranged between the two second filters 27. The fluorescence detection member 21 is arranged in the fourth light transmission hole 23 and located at the end of the fourth light transmission hole 24 away from the third light transmission hole 23.

[0160] After the parallel light emitted by the light source providing device 1 enters the optical channel box 2, it first enters the second light transmission hole 22, passes through the first filter 26 to filter out stray light, is reflected at the second beam splitter 25 to enter the third light transmission hole 23, and is converged by the second converging lens 28 to finally transmit into the light transmission pipeline 5. After the excitation light is conducted to the microfluidic chip 6 through the light transmission pipeline 5, the substance in the reaction cavity 61 generates fluorescence. The fluorescence is conducted into the third light transmission hole 23 of the optical channel box 2 again through the light transmission pipeline 5. The fluorescence is first shaped by the second converging lens 28, is perpendicularly incident on the second beam splitter 25 and passes through a specific waveband, then passes through a second filter 27 to filter out stray light for the first time, is converged by the third converging lens 29, and a light spot with a size conforming to the size of the light window of the fluorescence detection member 21 is obtained. The light spot passes through another second filter 27 to filter out stray light for the second time, and is detected by the fluorescence detection member 21. See Figure 11 It is a schematic diagram of simulation of light source transmission for the optical channel box 2.

[0161] Referring to Figure 5 The temperature control device 4 comprises a first heating member 41, a second heating member 42, a first heat conduction member 43, a second heat conduction member 44, a first heat insulation member 45, a second heat insulation member 46 and a fixing member 47.

[0162] The first heating member 41 is located between the first heat insulation member 45 and the first heat conduction member 43, and the second heating member 42 is located between the second heat insulation member 46 and the second heat conduction member 44. The first heat conduction member 43 and the second heat conduction member 44 have a receiving part for accommodating the chip 6. The fixing member 47 is used for fixing the light transmission pipeline 5. The light transmission pipeline 5 first passes through the fixing member 47, and then passes through the second heat insulation member 46, the second heating member 42 and the second heat conduction member 44 in sequence, and is aligned with the reaction cavity 61 on the chip 6. The first heat conduction member 43 comprises a side surface 431 opposite to the port of the light transmission pipeline 5, and a reflection curved surface 4311 is arranged on the side surface 431. The reflection curved surface 4311 is configured to reflect the excitation light source passing through the reaction cavity 61 to the reaction cavity 61.

[0163] The excitation light source in the light transmission pipeline 5 passes through the fixing member 47, the second heat insulation member 46, the second heating member 42 and the second heat conduction member 44 in sequence, and is directed to the reaction cavity 61 on the chip 6. Part of the excitation light source passes through the reaction cavity 61 and is incident on the first heat conduction member 43. The reflection curved surface 4311 on the first heat conduction member 43 reflects the excitation light source back to the reaction cavity 61. Through the reflection of the reflection curved surface 4311 of the first heat conduction member 43, the excitation light is twice irradiated in the reaction cavity 61, the fluorescent substance in the reaction cavity 61 is twice excited, and the fluorescent excitation efficiency and the signal-to-noise ratio are improved.

[0164] The optical channel box 2 and the temperature control device 4 are connected through the light transmission pipeline 5. The light transmission pipeline 5 is responsible for the transmission of the excitation light source and the transmission of the fluorescence excited by the substance in the microfluidic chip. Optionally, in the temperature control device 4, a through hole with a diameter of 1.4 mm is punched on the second heat insulation member 46, the second heat conduction member 44 and the second heating member 42 located in the 55° temperature zone, which is used for inserting the light transmission pipeline 5. The excitation light source emitted by the light transmission pipeline 5 enters the reaction cavity 61 of the chip 6 through direct incidence and reflection through the upper first heat conduction member 43, and the generated fluorescence is collected by the light transmission pipeline 5.

[0165] Because the size difference between the light transmission pipeline 5 and the reaction cavity 61 is too large, only a small part of the fluorescence is directly excited. Therefore, exciting more fluorescence has great significance for improving the collection of useful signals and the signal-to-noise ratio.

[0166] Therefore, the surface of the first heat-conducting member 43 is processed into a reflective curved surface (with an accuracy of ±0.01mm), and the curved surface is polished or coated to obtain the final product. Among them, the coating can be a high-reflective film with a reflectivity of more than 95%, and the material can be aluminum or silver. And a layer of SiO2 can be added to the surface of the coating to have an anti-oxidation effect. A temperature sensor is installed on the back of the first heat-conducting member 43. The temperature zone is monitored in real time by a computer and the temperature curve is adjusted to compensate for the heat loss caused by the structural deficiency of the first heat-conducting member 43, thereby avoiding the reduction of the amplification speed. It effectively takes into account the problems of uniformity of excitation light irradiation and slow temperature rise caused by heat loss.

[0167] The reflective curved surface 4311 of the first heat conducting member 43 may have various shapes, including a circular concave surface, an elliptical concave surface, or a strip-shaped concave surface, etc. Figures 12a to 12d , which is a schematic diagram of a simulation of reflection by four types of reflective curved surfaces 4311 arranged on the side of the first heat conducting member 43.

[0168] See also Figure 9 The microfluidic chip 6 includes a substrate 62 , a cover 63 , a sample injection port 64 , an exhaust port 65 , a liquid inlet channel 66 , an exhaust channel 67 , a first plug 68 and a second plug 69 .

[0169] The substrate 62 and the cover plate 63 are integrally connected by ultrasonic bonding. A reaction chamber 61, a liquid inlet channel 66, and an exhaust channel 67 are formed between the substrate 62 and the cover plate 63. The liquid inlet channel 66 and the exhaust channel 67 are respectively connected to the reaction chamber 61. The sample inlet 64 is connected to the liquid inlet channel 66, and the exhaust port 65 is connected to the exhaust channel 67. A first plug 68 is used to block the sample inlet 64. A second plug 69 is used to block the exhaust port 65.

[0170] The microfluidic chip 6 is made of transparent materials such as polycarbonate.

[0171] The reaction chamber 61 may be an amplification chamber. Optionally, the reaction chamber 61 is a square chamber of 4.5 mm*4.5 mm.

[0172] To fully utilize the excitation light without causing excessive heat loss that could affect the overall heating rate, a reflective curved surface 4311 is provided on the side 31 of the first heat conductor 43. The excitation light is emitted through the light transmission conduit 5 and, after being reflected by the reflective curved surface 4311 on the first heat conductor 43, evenly illuminates the reaction solution within the reaction chamber 61, filling the entire reaction chamber 61 and significantly increasing the total amount of fluorescence excited. Because fluorescence itself is a Lambertian light source, more of the excited fluorescence is reflected back into the light transmission conduit 5, increasing the total amount of fluorescence collected and improving the overall signal-to-noise ratio.

[0173] refer to Figures 1 to 4The switching mechanism 3 includes a bearing member 31, a rotating shaft 32, a power member 33, a base frame 34, a first wheel 35, a second wheel 36, a belt 37, a top plate 38 and a housing 39. The housing 39 includes a shell 391 and a chassis 392.

[0174] The functions of the switching mechanism 3 include: first, assembling various components, which is equivalent to the skeleton of the entire system; second, realizing the switching of the optical channel box 2 to complete multi-channel detection; third, it also plays a role in shading and avoiding optical crosstalk.

[0175] exist Figures 1 to 4 In the illustrated embodiment, six optical channel boxes 2 are inserted into corresponding channel slots on a carrier 31. A top plate 38 is placed on top of the optical channel boxes 2. The six holes in the outer ring of the top plate 38 are connected and locked with the six holes in the outer ring of the carrier 31 via threaded connectors, securing the boxes. The six holes in the inner ring of the top plate 38 mate with the third light holes 23 of the six optical channel boxes 2, allowing light to pass through. The six holes in the inner ring of the carrier 31 mate with the fourth light holes 24 of the six optical channel boxes 2, allowing light to pass through. The six fixed optical channel boxes 2, top plate 38, and carrier 31 are then inserted into a chassis 392. The light source device 1 is also placed into the chassis 392 and secured, locking it with the bottom threads. A rotating shaft 32 is inserted between the six optical channel boxes 2, and two bearings are provided on the rotating shaft 32 to facilitate rotation. Then, the outer shell 391 for shielding light is used to cover the entire structure from the outside. There are three threaded holes around the outer shell 391 that can lock the outer shell 391 and the chassis 392. At the same time, there is a small cylindrical protrusion on the upper part of the outer shell 391, and a hole is punched inside to insert and fix the light transmission pipeline 5. The rotating shaft 32 extends out of the carrier 31, the chassis 392 and the base frame 34 in sequence to connect to the second wheel 36. The power member 33 is locked with the base frame 34 by threads. The drive shaft of the power member 33 passes through the base frame 34 to connect to the first wheel 35. The first wheel 35 and the second wheel 36 are connected by a belt 37. The power member 33 includes a motor. The rotation of the motor can drive the first wheel 35, the second wheel 36, and the rotating shaft 32 to rotate, thereby driving the six optical channel boxes 2, the top plate 38 and the carrier 31 to rotate, completing the fluorescence detection of different channels.

[0176] Some embodiments further provide a microfluidic chip detection system, which includes a chip 6 and the above-mentioned fluorescence detection device.

[0177] The fluorescence detection device provided by the embodiment of the present application is a real-time fluorescence detection device under harsh condition ultrafast PCR, excitation light is emitted in parallel and collimated by the light source providing device 1, then the excitation light transmission and reflected fluorescence detection of corresponding channels are completed by at least two optical channel boxes 2. The excitation light is transmitted to the temperature control device 4 through the light transmission pipe 5, the optical system and the thermal system are combined, the excitation light is uniformly reflected twice by the reflecting curved surface, the total amount of excitation of the fluorescent substance is increased, and the signal-to-noise ratio and the detection lower limit of the system are improved. The switching mechanism 3 is used to complete the switching of the optical channel box 2, and the real-time fluorescence detection of multiple channels can be realized.

[0178] In some embodiments, the operation process of the microfluidic chip detection system is as follows:

[0179] The operator injects the reaction solution into the microfluidic chip 6, and blocks the sample inlet 64 and the exhaust port 65 with the first plug 68 and the second plug 69 respectively;

[0180] The light source providing device 1 turns on the light source providing piece 12 of the corresponding channel, and emits collimated parallel excitation light;

[0181] The parallel excitation light enters the optical channel box 2, the excitation light converges on the light transmission pipe 5 through the channel box, and the excitation light is transmitted to the temperature control device 4 through the light transmission pipe 5;

[0182] The excitation light passes through the reaction cavity 61 of the microfluidic chip 6 to complete the first excitation, then passes through the reflecting curved surface 4311 to complete the second excitation of the solution in the reaction cavity 61;

[0183] The fluorescence emitted by the solution is collected by the light transmission pipe 5, transmitted back to the optical channel box 2, and then incident on the fluorescence detection piece 21 after shaping, to complete the detection of this channel;

[0184] The switching mechanism 3 rotates the at least two optical channel boxes 2 to switch the next optical channel box 2, and starts the next round of detection cycle.

[0185] After the detection of the multiple optical channel boxes 2 is sequentially completed, the operator removes the chip 6.

[0186] Based on the above embodiments of the present application, the technical features of one embodiment can be beneficially combined with one or more other embodiments without explicit negation or conflict.

[0187] Although some specific embodiments of the present application have been described in detail by way of example with reference to the accompanying drawings, it is to be understood that the above examples are intended to be illustrative only and are not intended to limit the scope of the present application. It is to be appreciated that variations and modifications to the above embodiments can be made without departing from the scope and spirit of the present application. The scope of the present application is defined by the appended claims.

Claims

1. A fluorescence detection device, characterized in that: include: A light source providing device (1) is configured to provide a full spectrum light source; At least two optical channel boxes (2) are configured to selectively receive the full-spectrum light source provided by the light source providing device (1) and generate and emit an excitation light source; the optical channel box (2) is also configured to receive fluorescence generated by the excitation light source and perform fluorescence detection; a switching mechanism (3) configured to move the at least two optical channel boxes (2) so that one of the at least two optical channel boxes (2) receives the full-spectrum light source provided by the light source providing device (1); A temperature control device (4) comprising a receiving portion for mounting a chip (6), wherein the temperature control device (4) is configured to control the temperature within a reaction chamber (61) on the chip (6); and a light transmission pipeline (5) connecting the optical channel box (2) and the temperature control device (4), wherein the light transmission pipeline (5) is configured to transmit the excitation light source to the reaction chamber (61), and transmit the fluorescence generated by the excitation light source in the reaction chamber (61) to the optical channel box (2); The temperature control device (4) comprises: a first heating element (41) configured to heat the reaction chamber (61) of the chip (6); and A first heat-conducting member (43) is provided between the first heating member (41) and the accommodating portion, and the first heat-conducting member (43) includes a side surface (431) arranged opposite to the port of the light transmission pipeline (5), and a reflective curved surface (4311) is provided on the side surface (431), and the reflective curved surface (4311) is configured to reflect the excitation light source passing through the reaction chamber (61) back to the reaction chamber (61); The switching mechanism (3) comprises a carrier (31) and a rotating shaft (32), wherein the rotating shaft (32) passes through the carrier (31) and is configured to drive the carrier (31) to rotate, and the at least two optical channel boxes (2) are provided on the carrier (31) and arranged around the rotating shaft (32); The switching mechanism (3) further comprises: Power member (33); A bottom frame (34), on which the power member (33) and the bearing member (31) are spaced apart; A first wheel (35) and a second wheel (36) are provided below the base frame (34), wherein the first wheel (35) is connected to the power member (33), and the second wheel (36) is connected to the rotating shaft (32); and A belt (37) connects the first wheel (35) and the second wheel (36).

2. The fluorescence detection device according to claim 1, wherein The temperature control device (4) further comprises: a second heating element (42) disposed on a side of the accommodating portion opposite to the first heat conducting element (43), the second heating element (42) being configured to heat the reaction chamber (61) of the chip (6), the second heating element (42) being provided with a through hole allowing the light transmission pipeline (5) to pass through; and A second heat-conducting member (44) is provided between the accommodating portion and the second heating member (42), and the second heat-conducting member (44) is provided with a through hole allowing the light transmission pipeline (5) to pass through.

3. The fluorescence detection device according to claim 1 or 2, wherein: The light source providing device (1) comprises: A first light aperture (11) configured to transmit a full spectrum light source; and At least two light source providing components (12) are configured to selectively provide a full-spectrum light source to the first light hole (11).

4. The fluorescence detection device according to claim 3, wherein At least two light source providing components (12) include a first light source providing component (121) and a second light source providing component (122), wherein the transmission direction of the full-spectrum light source emitted by the first light source providing component (121) is consistent with the extension direction of the first light through hole (11), and the transmission direction of the full-spectrum light source emitted by the second light source providing component (122) has an angle greater than zero with the extension direction of the first light through hole (11); the light source providing device (1) also includes a first beam splitter (13), the first beam splitter (13) is obliquely arranged in the first light through hole (11), the first beam splitter (13) is configured to allow the full-spectrum light source provided by the first light source providing component (121) to pass through, and is configured to reflect the full-spectrum light source provided by the second light source providing component (122) so that its transmission direction is consistent with the extension direction of the first light through hole (11).

5. The fluorescence detection device according to claim 3, wherein The light source providing device (1) further comprises at least two first converging lenses (14), the at least two first converging lenses (14) being respectively arranged at the emission ends of the at least two light source providing components (12) in a one-to-one correspondence, and the first converging lenses (14) being configured to converge the full-spectrum light source provided by the light source providing components (12) and transmit the converged light to the first light passage (11).

6. The fluorescence detection device according to claim 3, wherein: The optical channel box (2) comprises: a fluorescence detection member (21), configured to perform fluorescence detection; A second light-through hole (22) is configured to communicate with the first light-through hole (11); a third light through hole (23) communicating with the second light through hole (22), the third light through hole (23) being configured to transmit an excitation light source to the outside of the optical channel box (2) and to receive fluorescence generated by the excitation light source and returned from the outside of the optical channel box (2); and A fourth light through hole (24) is connected to the third light through hole (23), and the fourth light through hole (24) is configured to guide the fluorescence to the fluorescence detection element (21).

7. The fluorescence detection device according to claim 6, wherein: The connecting portion of the third light through hole (23) is connected to the second light through hole (22) and the fourth light through hole (24) respectively. The optical channel box (2) further comprises a second beam splitter (25). The second beam splitter (25) is arranged obliquely on the connecting portion. The second beam splitter (25) is configured to allow the excitation light source transmitted by the second light through hole (22) to enter the third light through hole (23), and to allow the fluorescence transmitted by the third light through hole (23) to enter the fourth light through hole (24).

8. The fluorescence detection device according to claim 6, wherein: The third light through hole (23) and the fourth light through hole (24) are located in the same extension direction, and an angle greater than zero is formed between the extension direction of the second light through hole (22) and the extension direction of the third light through hole (23).

9. The fluorescence detection device according to claim 6, wherein: The optical channel box (2) further comprises: a first filter (26) disposed on the second light hole (22); and The second filter (27) is arranged on the fourth light hole (24).

10. The fluorescence detection device according to claim 9, wherein: The optical channel box (2) further comprises: a second converging lens (28), disposed at the third light hole (23); and / or The third converging lens (29) is arranged at the fourth light hole (24).

11. The fluorescence detection device according to claim 10, wherein: There are two second optical filters (27), and the third converging lens (29) is arranged between the two second optical filters (27).

12. The fluorescence detection device according to claim 6, wherein: It also includes a light transmission pipeline (5), which is configured to communicate with the third light-transmitting hole (23) to transmit the excitation light source provided by the optical channel box (2) or to transmit fluorescence to the optical channel box (2).

13. The fluorescence detection device according to claim 1 or 2, characterized in that: The at least two optical channel boxes (2) are arranged around a rotation axis.

14. The fluorescence detection device according to claim 1 or 2, characterized in that: The light source providing device (1) comprises a first light through hole (11) for transmitting a full-spectrum light source, and the optical channel box (2) comprises a second light through hole (22) and a third light through hole (23), wherein the second light through hole (22) is provided on the side of the optical channel box (2), and the second light through hole (22) is configured to communicate with the first light through hole (11), and the third light through hole (23) is provided on the top of the optical channel box (2), and the third light through hole (23) is configured to transmit an excitation light source outward, or transmit fluorescence generated by the excitation light source inward.

15. A microfluidic chip detection system, characterized in that: The device comprises a chip (6) and the fluorescence detection device according to any one of claims 1 to 14.

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