Synergistic composition for providing thousand grain weight of wheat

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a synergistic composition for providing thousand grain weight of wheat. The bactericidal composition is characterized in that effective components of the bactericidal composition are formed by compounding murraya paniculata ketone and prothioconazole, and the mass ratio of the murraya paniculata ketone to the prothioconazole is (1-20): (8-1). When the murrayone in the bactericidal composition is compounded with other effective components, the bactericidal composition has a synergistic effect on wheat powdery mildew pathogenic bacteria within a certain mass ratio range, the prevention and treatment effect on wheat powdery mildew can be improved, the influence of wheat powdery mildew on wheat thousand seed weight is reduced, and further the thousand seed weight of wheat can be increased. And secondly, when the murrayone in the bactericidal composition is compounded with other effective components, the development of drug resistance of pathogenic bacteria can be effectively delayed, so that the drug resistance risk caused by application of a single effective component can be reduced. In addition, the bactericidal composition can reduce the application amount of the effective components, reduce the prevention and treatment cost, and provide support for development of novel medicaments.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular to a synergistic composition for providing thousand-grain weight of wheat. Background Art

[0002] Wheat powdery mildew is a worldwide disease caused by Blumeria graminis f.sp.Tritici, which parasitizes and infects wheat. Small yellow spots can be seen on the diseased part at the early stage of the disease. As the disease worsens, the spots gradually develop into lesions with a layer of powdery mildew on the surface. When the disease is mild, the mildew spots are scattered; as the disease worsens, the mildew spots gradually expand into pieces and eventually cover the entire leaf; the lower part of the lesion and the surrounding tissues turn green, the diseased leaves turn yellow and wither early, and the involvement of the stems and leaf sheaths will cause the whole plant to fall over, the plant will be short and weak, the ears will be small and the grains will be few, and the thousand-grain weight will drop significantly, which will eventually affect the wheat yield.

[0003] In agricultural production, planting disease-resistant varieties is the most economical means of preventing and controlling wheat powdery mildew. However, due to the rapid reproduction and mutation of wheat powdery mildew fungi, disease-resistant varieties lose their disease resistance after a few years of large-scale planting. At present, the most important measure for preventing and controlling wheat powdery mildew is chemical control. Commonly used chemical agents include triazole agents such as triadimefon. However, due to the long-term irrational use of single-active ingredient chemical agents, powdery mildew has developed varying degrees of resistance to a variety of existing chemical agents. In order to improve the prevention and control effect of powdery mildew, reduce the impact of powdery mildew on wheat thousand-grain weight, and reduce the development speed of pathogen resistance, it is necessary to develop new agents.

[0004] Murraya ketone, CAS number: 19668-69-0, molecular formula: C 15 H 14 O4, comes from Rutaceae plants. Its molecular structure is as follows:

[0005]

[0006] CN201510815853.5 discloses the use of a natural compound, murrayon, as an agricultural fungicide. Its specification discloses that murrayon has preventive and therapeutic effects on a variety of diseases such as rapeseed sclerotinia rot, rice sheath blight, strawberry gray mold, wheat fusarium rust, pepper anthracnose and tomato early blight.

[0007] Compounding the active ingredients of different pesticides is a convenient way to develop new agents. The inventors found through indoor biological activity tests that compounding murrayon with metconazole, tebuconazole or prothioconazole has a synergistic effect on the prevention and control of wheat powdery mildew within a certain mass ratio range. At present, there are no reports on compounding murrayon with metconazole, tebuconazole or prothioconazole.

[0008] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0009] The object of the present invention is to provide a synergistic composition for improving the thousand-grain weight of wheat. Compared with a single active ingredient, the composition can improve the control effect of wheat powdery mildew, thereby helping to reduce the impact of powdery mildew on the thousand-grain weight of wheat, and at the same time can delay the development of pathogen resistance, thereby providing support for the development of new agents.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] The first object of the present invention is to provide a fungicide composition, wherein the active ingredients of the fungicide composition are compounded with murrayone and metconazole, tebuconazole or prothioconazole.

[0012] Preferably, the mass ratio of murrayone to metconazole is 1-5:20-1.

[0013] Preferably, the mass ratio of murrayolazone to tebuconazole is 1-40:15-1.

[0014] Preferably, the mass ratio of murrayone to prothioconazole is 1-20:8-1.

[0015] The second object of the present invention is to provide a fungicide, which comprises the fungicide composition and other auxiliary ingredients.

[0016] The third object of the present invention is to provide the use of the fungicidal composition in preventing and controlling wheat fungal diseases.

[0017] The fourth object of the present invention is to provide the use of the fungicide composition in preventing and controlling wheat powdery mildew.

[0018] The fifth object of the present invention is to provide the use of the bactericidal composition in increasing the thousand-grain weight of wheat.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) When murraya ketone is compounded with other effective ingredients in the fungicide combination of the present invention, it has a synergistic effect on wheat powdery mildew pathogens within a certain mass ratio range, can improve the control effect of wheat powdery mildew, reduce the impact of wheat powdery mildew on wheat thousand-grain weight, and thus help to increase wheat thousand-grain weight.

[0021] (2) When the murrayolazone in the fungicide composition of the present invention is compounded with other effective ingredients, the development of drug resistance of pathogens can be effectively delayed, thereby reducing the risk of drug resistance caused by the use of a single effective ingredient.

[0022] (3) The fungicidal composition of the present invention can reduce the application amount of the active ingredient, reduce the cost of prevention and treatment, and provide support for the development of new agents. DETAILED DESCRIPTION

[0023] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0024] Example :Indoor biological activity test of murraya ketone after compounding

[0025] 1. Test crops: "Zhengmai 9023" wheat seeds, potted and cultured to the 2-3 true leaf stage, 10 plants per pot, numbered and reserved.

[0026] 2. Test reagents

[0027] 98% Murraya ketone technical (Shanghai Yuanye Biotechnology Co., Ltd.)

[0028] 95% metconazole technical (Shanghai Heteng Fine Chemical Co., Ltd.)

[0029] 97% Tebuconazole technical (Shenyang Kechuang Chemicals Co., Ltd.)

[0030] 95% Prothioconazole technical (Ningxia Yifan Biotechnology Co., Ltd.)

[0031] 3. Test method (refer to NY / T 1156.4-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 4: Pot Plant Method for Control of Wheat Powdery Mildew")

[0032] 3.1 Preparation of the drug: dissolve the original drug with dimethyl sulfoxide, dilute it with a 0.1% Tween-80 solution in water, prepare the single-dose stock solution, set multiple groups of proportions, and set 5 series of mass concentration gradients for each single dose and each group of mixed doses;

[0033] 3.2 Inoculation treatment: The fresh spores of powdery mildew (Blumeria graminis f. sp. Tritici) produced on the diseased wheat leaves within 24 hours were evenly shaken off and inoculated on the test wheat seedlings;

[0034] 3.3 Chemical treatment: After 24 hours, spray the solution evenly on the leaves of the test wheat seedlings until they are completely wet. Three pots are used for each treatment, and a treatment containing only 0.1% Tween-80 aqueous solution is set as a blank control; after natural air drying, move to a constant temperature room and continue to cultivate at a temperature of 22°C and light:darkness = 16h:8h.

[0035] 3.4 Investigation and calculation: When the diseased leaf rate of the blank control reaches more than 80%, investigate the disease situation of each treatment according to the grading standard in Table 1. Calculate the disease index and control effect of each treatment based on the investigation data. The grading standard is shown in Table 1.

[0036] Table 1 Disease grade assessment criteria

[0037]

[0038]

[0039]

[0040] 3.5 Data analysis: DPS software was used for data statistical analysis. The logarithmic value of fungicide concentration was taken as x and the corresponding probability value of prevention effect was taken as y for linear regression. The toxicity regression equation and the toxicity EC value of the agent to the target pathogen were obtained. 50 The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

[0041] 3.6. Pharmacological evaluation: The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient. CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 2-4.

[0042] Table 2 Indoor biological activity test results of the combination of murrayaceae and metconazole against wheat powdery mildew

[0043]

[0044] As shown in Table 2, within the mass ratio of 1-5:20-1, the co-toxicity coefficient of the combination of murrayolazone and metconazole against wheat powdery mildew was greater than 120, showing a synergistic effect.

[0045] Table 3 Indoor biological activity test results of the combination of styraconazole and tebuconazole against wheat powdery mildew

[0046]

[0047] As shown in Table 3, within the mass ratio of 1-40:15-1, the co-toxicity coefficient of the combination of murrayolazone and tebuconazole against wheat powdery mildew was greater than 120, showing a synergistic effect.

[0048] Table 4 Indoor biological activity test results of the combination of murrayaceae and prothioconazole against wheat powdery mildew

[0049]

[0050] As shown in Table 4, within the mass ratio of 1-20:8-1, the co-toxicity coefficient of the combination of murrayone and prothioconazole against wheat powdery mildew was greater than 120, showing a synergistic effect.

[0051] In summary, when murraykonium ketone in the fungicide combination of the present invention is compounded with metconazole, tebuconazole or prothioconazole, it has a synergistic effect on wheat powdery mildew pathogens within a certain mass ratio range, can improve the control effect on wheat powdery mildew, reduce the influence of wheat powdery mildew on the thousand-grain weight of wheat, and thus help to increase the thousand-grain weight of wheat.

[0052] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A bactericidal composition, characterized in that: The effective ingredient of the bactericidal composition is prepared by compounding murrayone and prothioconazole, and the mass ratio of murrayone to prothioconazole is 1-20:8-1.

2. A bactericide, characterized in that: The bactericide comprises the bactericidal composition according to claim 1 and other auxiliary ingredients.

3. Use of the fungicide composition according to claim 1 in preventing and controlling wheat powdery mildew.

4. Use of the bactericidal composition according to claim 1 in increasing the thousand-grain weight of wheat.

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