Equipment for establishing mouse hemorrhagic shock resuscitation model

Abstract

The invention discloses equipment for establishing a mouse hemorrhagic shock resuscitation model. The equipment comprises a surgery operation table, a body temperature maintaining device, a puncture module, an in-vitro blood heat preservation device, an artery puncture device, a blood transfusion / bloodletting device, a blood temporary storage device, an arterial blood pressure monitoring device and a three-way device. According to the invention, venipuncture catheterization is not needed in the blood transfusion / letting process, the purposes of blood transfusion / letting and real-time arterial blood pressure monitoring can be achieved only through arterial puncture catheterization, and the retention of veins can not only reduce the difficulty of the operation, but also ensure the backflow of tissue blood after the operation, so that the injury caused by the operation is reduced. According to the in-vitro blood heat preservation device, the in-vitro blood can be kept at a constant temperature, and the death risk of mice caused by body temperature changes can be effectively reduced. According to the blood transfusion / bloodletting device, the blood transfusion / bloodletting speed can be manually controlled, the problem that the blood transfusion / bloodletting speed cannot be controlled by manual bloodletting can be effectively solved, and heart failure caused by rapid and massive blood transfusion is avoided.

Description

Technical Field

[0001] The invention relates to the field of establishing basic experimental animal models, in particular to a device for establishing a mouse hemorrhagic shock resuscitation model. Background Art

[0002] Hemorrhagic shock is a common clinical emergency. When insufficient blood volume exceeds the compensatory function, shock syndrome will appear. Timely reperfusion of ischemic tissues and restoration of normal blood circulation are the key to treatment. However, both ischemia and reperfusion will cause a series of cell and tissue damage. Therefore, the hemorrhagic shock resuscitation model has great research value. However, mice are small in size and have thin blood vessels. They have poor tolerance to more traumatic surgeries such as hemorrhagic shock resuscitation. Therefore, modeling is difficult and the postoperative mortality rate is high. The research results of this model in mice are very scarce compared with rats. However, compared with rats, mice have irreplaceable advantages such as short growth cycle, strong reproductive ability, and can be used for gene knockout. Therefore, inventing a device that can reduce the difficulty of modeling and improve the success rate of modeling to optimize the modeling method has become an urgent problem to promote the research progress of hemorrhagic shock resuscitation injury. Summary of the invention

[0003] The purpose of the present invention is to provide a device for establishing a mouse hemorrhagic shock resuscitation model, so that during the blood transfusion / bloodletting process, there is no need for venous puncture and catheterization, and the purpose of blood transfusion / bloodletting and real-time arterial blood pressure monitoring can be achieved only by arterial puncture and catheterization. The preservation of the vein can not only reduce the difficulty of the operation, but also ensure the return of tissue blood after the operation, so as to reduce the damage caused by the operation. The present invention can maintain the body temperature of the mouse and the temperature of the ex vivo blood, and ensure that the speed of blood transfusion / bloodletting is slow and uniform, so that the difficulty of establishing a mouse hemorrhagic shock resuscitation model and the mortality rate of modeling are greatly reduced.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] A device for establishing a hemorrhagic shock resuscitation model in mice, comprising an operating table, a body temperature maintenance device, an extracorporeal blood insulation device, an arterial puncture device, a blood transfusion / bloodletting device, a blood temporary storage device, an arterial blood pressure monitoring device, and a three-way device. The operating table comprises a mouse gas anesthesia device, a guide rail, and a fixed column. The mouse gas anesthesia device needs to be connected to an external anesthesia machine for use and is used for gas anesthesia of mice during surgery. The guide rail is used to install the puncture module, and the fixed column is used to fix the limbs of the mouse. The body temperature maintenance device comprises a heating blanket, a rectal temperature detection probe, and a temperature controller. The heating blanket is used to heat the mouse when the mouse body temperature is lower than a preset temperature. The rectal temperature detection probe is used to feed back the mouse body temperature to the temperature controller. The temperature controller can preset a temperature. When the mouse body temperature fed back by the rectal temperature detection probe is lower than the preset temperature, the heating blanket starts heating. When the rectal temperature detection probe feeds back the mouse body temperature, the heating blanket starts heating. When the body temperature of the mouse fed back by the detection probe reaches the preset temperature, the heating blanket stops working. The extracorporeal blood insulation device includes a constant temperature heating device and a temperature display panel. The arterial puncture device is used for arterial puncture during the modeling process. The blood transfusion / bloodletting device is used to push / pull the syringe at a uniform speed at an artificially set speed to achieve the purpose of uniform blood transfusion / bloodletting. The blood temporary storage device is used for temporary storage of the released blood. It is pre-filled with heparin for anticoagulation, and its outer side is in close contact with the constant temperature heating device. The arterial blood pressure monitoring device is used for real-time arterial blood pressure monitoring to guide the amount of blood transfusion / bloodletting. The three-way device connects the arterial puncture device, the blood temporary storage device, and the arterial blood pressure monitoring device to achieve the purpose of blood transfusion / bloodletting and real-time arterial blood pressure monitoring without the need for venous puncture and catheterization during the transfusion / bloodletting process. The lower end of the three-way device is fixed to the extracorporeal blood insulation device by a detachable buckle.

[0006] As a further solution of the present invention: the surgical operating table includes a mouse gas anesthesia device, a guide rail, and a fixed column. The mouse gas anesthesia device needs to be connected to an external anesthesia machine for use in gas anesthesia of mice during surgery. The guide rail is used to install the puncture module, and the fixed column is used to fix the mouse limbs.

[0007] As a further solution of the present invention: the body temperature maintaining device includes a heating blanket, a rectal temperature detection probe, and a temperature controller. The heating blanket is used to heat the mouse when the mouse body temperature is lower than a preset temperature. The rectal temperature detection probe is used to feed back the mouse body temperature to the temperature controller. The temperature controller can preset a temperature. When the mouse body temperature fed back by the rectal temperature detection probe is lower than the preset temperature, the heating blanket starts heating. When the mouse body temperature fed back by the rectal temperature detection probe reaches the preset temperature, the heating blanket stops working.

[0008] As a further solution of the present invention: the extracorporeal blood insulation device includes a constant temperature heating device, a temperature control device, and a temperature display panel. The temperature control device can be adjusted by buttons to allow the constant temperature heating device to maintain a set temperature. The temperature display panel can display the real-time temperature of the constant temperature heating device. The temperature can be set in advance through the temperature control device before modeling, and modeling can begin after the temperature display panel reaches the preset temperature.

[0009] As a further solution of the present invention: the arterial puncture device includes a stainless steel puncture guide needle core, a hose, a needle seat, a needle handle, and an infusion tube. Under the guidance of the stainless steel puncture guide needle core, the hose is inserted into the mouse blood vessel. After the stainless steel puncture guide needle core is withdrawn, the hose is retained in the blood vessel for subsequent modeling infusion / bloodletting steps. The needle handle is connected to the stainless steel puncture guide needle core for easy handholding, and the needle seat connects the hose and the infusion tube.

[0010] As a further solution of the present invention: the blood transfusion / bloodletting device includes a card slot, a driving device, and a control panel. The card slot is driven by the driving device. The control panel is manipulated to move the card slot forward and backward at a uniform speed to achieve a uniform blood transfusion / bloodletting process, thereby accurately controlling the mouse arterial blood pressure.

[0011] As a further solution of the present invention: the blood temporary storage device includes a needle and a syringe, the syringe is pre-filled with heparin for temporarily storing mouse blood, and the needle is 0.45mm in size, which can effectively filter larger blood clots and reduce the mortality rate of mice.

[0012] As a further solution of the present invention: the three-way valve connects the arterial puncture device, the blood temporary storage device, and the arterial blood pressure monitoring device, so as to achieve the purpose of blood transfusion / blood letting and real-time arterial blood pressure monitoring by only arterial puncture and catheterization without the need for venous puncture and catheterization during the transfusion / blood letting process, and the lower end thereof is fixed to the extracorporeal blood insulation device by a detachable buckle.

[0013] As a further solution of the present invention: the arterial blood pressure monitoring device uses the Zhongshi Technology Bio-informatization Medical Signal Acquisition and Processing System, and the monitoring software is the Bio-informatization Medical Signal Acquisition and Processing System ZS2.7.

[0014] As a further solution of the present invention: a device for establishing a mouse hemorrhagic shock resuscitation model includes the following use process:

[0015] Step 1: At the beginning of modeling, the puncture module can be removed along the guide rail, the mouse's limbs can be fixed to the fixed column with rubber bands, the rectal temperature probe can be inserted into the mouse's anus, the body temperature maintenance device can be turned on, and the preset temperature can be set by adjusting the temperature controller. After the artery is separated, the puncture module can be installed along the guide rail, and the position can be adjusted and fixed according to the surgical site.

[0016] Step 2: Turn on the power switch of the extracorporeal blood warming device to preheat the device, and wait until the temperature display panel reaches the preset temperature before proceeding with subsequent operations. In addition, the arterial puncture device, the blood temporary storage device, the arterial blood pressure monitoring device, and the three-way device should all be used with heparin saline to exhaust the gas in the circuit before use to prevent air embolism from causing death of mice.

[0017] Step 3: When performing arterial puncture and catheterization, hold the needle handle to perform puncture. After the stainless steel puncture guide needle core and the hose enter the artery, fix the needle seat by hand, withdraw the stainless steel puncture guide needle core, and fix the infusion tube with tape to prevent the hose from falling off due to pulling.

[0018] Step 4: Turn on the arterial blood pressure monitoring device, monitor and record the real-time blood pressure through the bio-informatics medical signal acquisition and processing system ZS2.7 to guide the amount of blood transfusion / blood removal.

[0019] Step 5: Inject heparinized saline into the mouse through the blood transfusion / bleeding device and the blood temporary storage device to make it systemically heparinized, and then operate the control panel to perform bleeding at the set speed until the arterial blood pressure monitoring device shows that the real-time arterial blood pressure drops to the target range (35-45 mmHg), stop bleeding, maintain the blood pressure for 15 minutes (if the blood pressure fluctuates during this period, the control panel can be operated at any time to adjust it), and continue to perform blood transfusion at the set speed until the arterial blood pressure monitoring device shows that the real-time arterial blood pressure returns to normal blood pressure, then operate the control panel to stop blood transfusion, maintain normal blood pressure for 15 minutes, ligate the artery, withdraw the hose, and turn off all equipment.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The multiple tools and equipment required for establishing the mouse hemorrhagic shock resuscitation model are integrated into one, which reduces the problem of puncture needle falling off caused by pulling and twisting of the pipeline during operation, greatly reduces the difficulty of modeling, and improves the success rate of modeling.

[0022] 2. The present invention can achieve the purpose of blood transfusion / bloodletting and real-time arterial blood pressure monitoring by only arterial puncture and catheterization without the need for venous puncture and catheterization during the transfusion / bloodletting process. The preservation of veins can not only reduce the difficulty of surgery, but also ensure the return of tissue blood after surgery, reducing the damage caused by surgery.

[0023] 3. The body temperature maintenance device and extracorporeal blood insulation device of the present invention can maintain the mouse body temperature and extracorporeal blood at a set temperature. Since the model mice require a large amount of blood transfusion, the device can effectively reduce the risk of death of mice due to changes in body temperature.

[0024] 4. The blood transfusion / bloodletting device of the present invention can transfuse / bleed at a uniform speed, which can effectively solve the problem of inability to control the bloodletting speed during manual bloodletting, and can effectively avoid heart failure caused by rapid and massive blood transfusion during the blood transfusion stage.

[0025] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The figure is a schematic diagram of the structure of a device for establishing a mouse hemorrhagic shock resuscitation model;

[0027] Figure 2 The figure is a schematic diagram of the structure of an extracorporeal blood warming device in a device for establishing a mouse hemorrhagic shock resuscitation model;

[0028] Figure 3 The figure is a schematic diagram of the structure of an arterial puncture device in a device for establishing a mouse hemorrhagic shock resuscitation model;

[0029] Figure 4 The figure is a schematic diagram of the structure of a blood transfusion / blood-letting device in a device for establishing a mouse hemorrhagic shock resuscitation model;

[0030] Figure 5 The figure is a schematic diagram of the structure of a blood temporary storage device in an apparatus for establishing a mouse hemorrhagic shock resuscitation model.

[0031] In the figure: 1. operating table; 11. mouse gas anesthesia device; 12. guide rail; 13. fixed column; 2. body temperature maintenance device; 21. heating blanket; 22. rectal temperature detection probe; 23. temperature controller; 3. puncture module; 4. extracorporeal blood insulation device; 41. constant temperature heating device; 42. temperature display panel; 43. temperature regulating device; 5. arterial puncture device; 51. stainless steel puncture guide needle core; 52. hose; 53. needle seat; 54. needle handle; 55. infusion tube; 6. blood transfusion / blood letting device; 61. card slot; 62 track; 63. control panel; 7. blood temporary storage device; 71. needle; 72. syringe; 8. arterial blood pressure monitoring device; 9. three-way device. DETAILED DESCRIPTION

[0032] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the present invention will be described in detail below with reference to the accompanying drawings.

[0033] Embodiment 1:

[0034] See also Figure 1-Figure 5In an embodiment of the present invention, a device for establishing a mouse hemorrhagic shock resuscitation model comprises a surgical operating table (1), a body temperature maintenance device (2), a puncture module (3), an extracorporeal blood insulation device (4), an arterial puncture device (5), a blood transfusion / bloodletting device (6), a blood temporary storage device (7), an arterial blood pressure monitoring device (8), and a three-way device (9). The surgical operating table (1) comprises a mouse gas anesthesia device (11) and a guide rail (12). The mouse gas anesthesia device (11) needs to be connected to an external anesthesia machine and is used for mouse gas anesthesia during surgery. The guide rail (12) is used to install the puncture module (3). The body temperature maintenance device (2) comprises a heating blanket (21), a rectal temperature detection probe (22), and a temperature controller (23). The blanket (21) is used to heat the mouse when the mouse body temperature is lower than a preset temperature. The rectal temperature detection probe (22) is used to feed back the mouse body temperature to the temperature controller (23). The temperature controller (23) can preset a temperature. When the mouse body temperature fed back by the rectal temperature detection probe (22) is lower than the preset temperature, the heating blanket (21) starts heating. When the mouse body temperature fed back by the rectal temperature detection probe (22) reaches the preset temperature, the heating blanket (21) stops working. The puncture module (3) can be fixed at any position of the guide rail (12). The puncture module (3) can be installed on the surgical operating table (1) through the guide rail (12). According to the size of the mouse, the puncture device can be fixed at any position of the guide rail (12). The puncture device integrates an extracorporeal blood insulation device (4), an anatomic blood circulation device (5), and a blood circulation device (6). The arterial puncture device (5), the blood transfusion / bloodletting device (6), the blood temporary storage device (7), the arterial blood pressure monitoring device (8), and the three-way device (9). The extracorporeal blood insulation device (4) includes a constant temperature heating device (41), a temperature display panel (42), and a temperature regulating device (43). The required temperature can be set by the temperature regulating device (43). The temperature display panel (42) can display the temperature of the constant temperature heating device (41) in real time. Before modeling, the temperature can be set in advance by the temperature regulating device (43). After the temperature display panel (42) reaches the preset temperature, the modeling starts. The arterial puncture device (5) includes a stainless steel puncture guide needle core (51), a hose (52), a needle seat (53), a needle handle (54), and an infusion tube (55). Under the guidance of the stainless steel puncture guide needle core (51), the soft tube (52) is inserted into the blood vessel of the mouse. After the stainless steel puncture guide needle core (51) is withdrawn, the soft tube (52) is left in the blood vessel for the subsequent blood transfusion / bloodletting step of modeling. The needle handle (54) is connected to the stainless steel puncture guide needle core (51) for easy handholding. The needle seat (53) connects the soft tube (52) and the infusion tube (55). The blood transfusion / bloodletting device (6) includes a card slot (61), a track (62), and a control panel (63). The card slot (61) is used to clamp the syringe handle of the blood temporary storage device (7). By operating the control panel (63), the card slot (61) is moved forward and backward at a uniform speed along the track (62) to achieve a uniform blood transfusion / bloodletting process, thereby accurately controlling the arterial blood pressure of the mouse.The blood temporary storage device (7) includes a needle (71) and a syringe (72). The syringe (72) is pre-filled with heparin for temporarily storing mouse blood. The outer side of the syringe (72) is in close contact with the constant temperature heating device (41). The needle (71) is 0.45 mm in size and can effectively filter larger blood clots and reduce the mortality rate of mice. The arterial blood pressure monitoring device (8) uses the Bio-informatics Medical Signal Acquisition and Processing System of Zhongshi Technology. The monitoring software is the Bio-informatics Medical Signal Acquisition and Processing System ZS2.7. The three-way device (9) connects the arterial puncture device (5), the blood temporary storage device (7), and the arterial blood pressure monitoring device (8) to achieve the purpose of blood transfusion / bloodletting and real-time arterial blood pressure monitoring without the need for venous puncture and catheterization during the blood transfusion / bloodletting process. The lower end of the three-way device (9) is fixed to the extracorporeal blood insulation device (41) by a detachable buckle.

[0035] like Figure 1-Figure 3 The present invention also provides a use process of a device for establishing a mouse hemorrhagic shock resuscitation model, and the specific steps are as follows:

[0036] Step 1: At the beginning of modeling, the puncture module can be removed along the guide rail, the mouse's limbs can be fixed to the fixed column with rubber bands, the rectal temperature probe can be inserted into the mouse's anus, the body temperature maintenance device can be turned on, and the preset temperature can be set by adjusting the temperature controller. After the artery is separated, the puncture module can be installed along the guide rail, and the position can be adjusted and fixed according to the surgical site.

[0037] Step 2: Turn on the power switch of the extracorporeal blood warming device to preheat the device, and wait until the temperature display panel reaches the preset temperature before proceeding with subsequent operations. In addition, the arterial puncture device, the blood temporary storage device, the arterial blood pressure monitoring device, and the three-way device should all be used with heparin saline to exhaust the gas in the circuit before use to prevent air embolism from causing death of mice.

[0038] Step 3: When performing arterial puncture and catheterization, hold the needle handle to perform puncture. After the stainless steel puncture guide needle core and the hose enter the artery, fix the needle seat by hand, withdraw the stainless steel puncture guide needle core, and fix the infusion tube with tape to prevent the hose from falling off due to pulling.

[0039] Step 4: Turn on the arterial blood pressure monitoring device, monitor and record the real-time blood pressure through the bio-informatics medical signal acquisition and processing system ZS2.7 to guide the amount of blood transfusion / blood removal.

[0040] Step 5: Inject heparinized saline into the mouse through the blood transfusion / bleeding device and the blood temporary storage device to make it systemically heparinized, and then operate the control panel to perform bleeding at the set speed until the arterial blood pressure monitoring device shows that the real-time arterial blood pressure drops to the target range (35-45 mmHg), stop bleeding, maintain the blood pressure for 15 minutes (if the blood pressure fluctuates during this period, the control panel can be operated at any time to adjust it), and continue to perform blood transfusion at the set speed until the arterial blood pressure monitoring device shows that the real-time arterial blood pressure returns to normal blood pressure, then operate the control panel to stop blood transfusion, maintain normal blood pressure for 15 minutes, ligate the artery, withdraw the hose, and turn off all equipment.

[0041] In summary, the present invention integrates a variety of tools and equipment required for establishing a mouse hemorrhagic shock resuscitation model, reduces the problem of puncture needle falling off caused by pulling and twisting the pipeline during operation, greatly reduces the difficulty of modeling, and improves the success rate of modeling. The present invention can achieve the purpose of blood transfusion / bloodletting and real-time arterial blood pressure monitoring without the need for venous puncture and catheterization during the transfusion / bloodletting process, and only through arterial puncture and catheterization. The retention of veins can not only reduce the difficulty of surgery, but also ensure the return of tissue blood after surgery, reducing the damage caused by surgery. The body temperature maintenance device and the extracorporeal blood insulation device of the present invention can maintain the mouse body temperature and extracorporeal blood at a set temperature. Since the model mouse has a large blood transfusion volume, the device can effectively reduce the risk of death of mice caused by changes in body temperature. The blood transfusion / bloodletting device in the present invention can transfuse / bleed at a uniform speed, which can effectively solve the problem that the bloodletting speed cannot be controlled by artificial bloodletting during the bloodletting stage, and can effectively avoid heart failure caused by rapid and large-scale blood transfusion during the blood transfusion stage.

[0042] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. According to the content of this specification, many modifications and changes can be made. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A device for establishing a mouse hemorrhagic shock resuscitation model, characterized in that: It comprises a surgical operating table (1), a body temperature maintaining device (2), a puncture module (3), an extracorporeal blood warming device (4), an arterial puncture device (5), a blood transfusion / blood-letting device (6), a blood temporary storage device (7), an arterial blood pressure monitoring device (8), and a three-way device (9); The surgical operating table (1) comprises a mouse gas anesthesia device (11), a guide rail (12), and a fixing column (13). The mouse gas anesthesia device (11) needs to be connected to an external anesthesia machine for use in mouse gas anesthesia during surgery. The guide rail (12) is used to install the puncture module (3). The body temperature maintenance device (2) comprises a heating blanket (21), a rectal temperature detection probe (22), and a temperature controller (23). The heating blanket (21) is used to heat the mouse when the mouse body temperature is lower than a preset temperature. The rectal temperature detection probe (22) is used to feed back the mouse body temperature to the temperature controller (23). The temperature controller (23) can preset a temperature. When the mouse body temperature fed back by the rectal temperature detection probe (22) is lower than the preset temperature, the temperature controller (23) is set to a preset temperature. When the temperature is set, the heating blanket (21) starts heating. When the body temperature of the mouse fed back by the rectal temperature detection probe (22) reaches the preset temperature, the heating blanket (21) stops working. The puncture module (3) can be installed on the surgical operating table (1) through the guide rail (12), and according to the body size of the mouse, the puncture module (3) can be fixed at any position of the guide rail (12). The puncture module (3) can be installed on the surgical operating table (1) through the guide rail (12), and according to the body size of the mouse, the puncture device can be fixed at any position of the guide rail (12). The device integrates an extracorporeal blood insulation device (4), an arterial puncture device (5), a blood transfusion / bloodletting device (6), and a blood temporary storage device. (7), an arterial blood pressure monitoring device, (8) a three-way device (9), the extracorporeal blood insulation device (4) comprises a constant temperature heating device (41), a temperature display panel (42), and a temperature regulating device (43), the desired temperature can be set by the temperature regulating device (43), the temperature display panel (42) can display the temperature of the constant temperature heating device (41) in real time, the arterial puncture device (5) comprises a stainless steel puncture guide needle core (51), a hose (52), a needle seat (53), a needle handle (54), and an infusion tube (55), under the guidance of the stainless steel puncture guide needle core (51), the hose (52) is inserted into the mouse blood vessel, and after the stainless steel puncture guide needle core (51) is withdrawn, the hose (54) is inserted into the mouse blood vessel. 2) is retained in the blood vessel for the subsequent blood transfusion / bloodletting step of modeling. The needle handle (54) is connected to the stainless steel puncture guide needle core (51) for easy hand-holding. The needle seat (53) connects the hose (52) and the infusion tube (55). The blood transfusion / bloodletting device (6) includes a card slot (61), a track (62), and a control panel (63). The card slot (61) is used to clamp the syringe handle of the blood temporary storage device (7). By manipulating the control panel (63), the card slot (61) is moved forward and backward along the track (62) at a uniform speed to achieve a uniform blood transfusion / bloodletting process, thereby accurately controlling the arterial blood pressure of mice. The blood temporary storage device (7) includes a needle (71) and a syringe (72).The syringe (72) is pre-filled with heparin for temporarily storing mouse blood. Its outer side is in close contact with the constant temperature heating device (41). The needle (71) is 0.45 mm in size and can effectively filter larger blood clots to reduce the mortality rate of mice. The arterial blood pressure monitoring device (8) is used for real-time arterial blood pressure monitoring to guide the amount of blood transfusion / bloodletting. The three-way device (9) connects the arterial puncture device (5), the blood temporary storage device (7), and the arterial blood pressure monitoring device (8) to achieve the purpose of blood transfusion / bloodletting and real-time arterial blood pressure monitoring without the need for intravenous puncture and catheterization during the blood transfusion / bloodletting process. The lower end of the syringe is fixed to the extracorporeal blood insulation device (41) by a detachable buckle.

2. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The surgical operating table (1) comprises a mouse gas anesthesia device (11) and a guide rail (12). The mouse gas anesthesia device (11) needs to be connected to an external anesthesia machine and is used for gas anesthesia of mice during surgery. The guide rail (12) is used to install the puncture module (3).

3. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The body temperature maintaining device (2) comprises a heating blanket (21), a rectal temperature detection probe (22), and a temperature controller (23). The heating blanket (21) is used to heat the mouse when the body temperature of the mouse is lower than a preset temperature. The rectal temperature detection probe (22) is used to feed back the body temperature of the mouse to the temperature controller (23). The temperature controller (23) can be set to a preset temperature. When the body temperature of the mouse fed back by the rectal temperature detection probe (22) is lower than the preset temperature, the heating blanket (21) starts heating. When the body temperature of the mouse fed back by the rectal temperature detection probe (22) reaches the preset temperature, the heating blanket (21) stops working.

4. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The puncture module (3) can be fixed at any position of the guide rail (12), and the puncture module (3) can be installed on the operating table (1) through the guide rail (12). In addition, according to the size of the mouse, the puncture device can be fixed at any position of the guide rail (12), and it integrates an extracorporeal blood insulation device (4), an arterial puncture device (5), a blood transfusion / bloodletting device (6), a blood temporary storage device (7), an arterial blood pressure monitoring device, (8) and a three-way device (9).

5. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The extracorporeal blood warming device (4) comprises a constant temperature heating device (41), a temperature display panel (42), and a temperature regulating device (43). The desired temperature can be set by means of the temperature regulating device (43), and the temperature display panel (42) can display the temperature of the constant temperature heating device (41) in real time. Before modeling, the temperature can be set in advance by means of the temperature regulating device (43), and modeling can begin after the temperature display panel (42) reaches the preset temperature.

6. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The arterial puncture device (5) comprises a stainless steel puncture guide needle core (51), a soft tube (52), a needle seat (53), a needle handle (54), and an infusion tube (55). Under the guidance of the stainless steel puncture guide needle core (51), the soft tube (52) is inserted into the mouse blood vessel. After the stainless steel puncture guide needle core (51) is withdrawn, the soft tube (52) is left in the blood vessel for the subsequent infusion / bloodletting steps of modeling. The needle handle (54) is connected to the stainless steel puncture guide needle core (51) for easy handholding. The needle seat (53) connects the soft tube (52) and the infusion tube (55).

7. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The blood transfusion / bloodletting device (6) comprises a card slot (61), a track (62), and a control panel (63); the card slot (61) is used to clamp the syringe handle of the blood temporary storage device (7); the card slot (61) is moved forward and backward along the track (62) at a uniform speed by controlling the control panel (63), thereby achieving a uniform blood transfusion / bloodletting process, thereby accurately controlling the arterial blood pressure of mice.

8. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The blood temporary storage device (7) comprises a needle (71) and a syringe (72). The syringe (72) is pre-filled with heparin for temporarily storing mouse blood, and its outer side is in close contact with the constant temperature heating device (41). The needle (71) is 0.45 mm in size and can effectively filter larger blood clots, thereby reducing the mortality rate of mice.

9. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The arterial blood pressure monitoring device (8) uses the Bio-informatics Medical Signal Acquisition and Processing System of Zhongshi Technology, and the monitoring software is the Bio-informatics Medical Signal Acquisition and Processing System ZS2.

7.

10. The device for establishing a mouse hemorrhagic shock resuscitation model according to claim 1, characterized in that: The three-way device (9) connects the arterial puncture device (5), the blood temporary storage device (7), and the arterial blood pressure monitoring device (8) to achieve the purpose of blood transfusion / bloodletting and real-time arterial blood pressure monitoring by only arterial puncture and cannulation without the need for venous puncture and cannulation during the blood transfusion / bloodletting process. The lower end of the three-way device (9) is fixed to the extracorporeal blood insulation device (41) by a detachable buckle.

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