Antibacterial moxibustion stick as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of moxibustion sticks, and particularly relates to an antibacterial moxibustion stick and a preparation method and application thereof.The antibacterial moxibustion stick is formed by rolling antibacterial moxa, antibacterial microcapsules are attached to the surface of the antibacterial moxa, and when the antibacterial moxibustion stick burns and the temperature reaches 400-600 DEG C, the skin of the antibacterial microcapsules is broken, and antibacterial essential oil is released. Compared with a traditional moxibustion stick, the antibacterial moxibustion stick has the advantages that the antibacterial effect of the antibacterial moxibustion stick on escherichia coli, staphylococcus aureus, bacillus subtilis and pseudomonas aeruginosa is more than two times that of the traditional moxibustion stick, and the effect is remarkable.

Description

Technical Field

[0001] The invention belongs to the technical field of moxibustion, and in particular relates to an antibacterial moxibustion strip and a preparation method and use thereof. Background Art

[0002] Moxa is a soft and fine cotton product obtained by repeatedly drying, pounding, crushing, and sifting out impurities and dust. Moxa is the raw material for making moxa sticks and the main material used in moxibustion. Its main effects include promoting blood circulation, warming the meridians and stopping bleeding, dispelling cold and relieving pain, and maintaining health. Mugwort leaves are rich in a variety of bioactive ingredients, among which volatile oil is the main effective ingredient group of mugwort leaves. Mugwort volatile oil has the characteristics of a broad antibacterial spectrum, strong antibacterial activity, good clinical efficacy, and few and mild adverse reactions. The main components of moxa smoke from burning moxa are furans, aromatics, esters and other compounds, which also have antibacterial, anti-aging and lipid metabolism regulating effects.

[0003] The quality of moxa is affected by the different picking time, origin, aging and storage conditions of moxa. Modern research shows that there is no obvious difference in the non-volatile components of moxa samples from different production areas, but there are obvious differences in volatile components. In addition, the process of making moxa from moxa is complicated and has poor stability. The quality of moxa prepared from different batches of moxa will vary greatly. Although the quality of old moxa is good, it is difficult to obtain due to its high time cost. In addition, as the effective ingredient and quality control indicator, the volatile oil of moxa itself has the disadvantages of being volatile and unstable. During the burning and use process, its stability is even more difficult to guarantee, which will seriously affect the performance of pharmacological effects such as antibacterial.

[0004] The present invention prepares an antibacterial moxibustion stick for the first time, which can solve at least one of the above problems. Summary of the invention

[0005] The present invention is proposed to solve at least one of the above problems.

[0006] The first aspect of the present invention provides an antibacterial moxibustion stick, which is rolled from antibacterial moxa, and antibacterial microcapsules are attached to the surface of the antibacterial moxa. When the antibacterial moxibustion stick burns and the temperature reaches 400-600°C, the antibacterial microcapsule skin ruptures to release antibacterial essential oil.

[0007] Preferably, the antibacterial essential oil includes wormwood essential oil, eucalyptus essential oil, tea tree essential oil, thyme essential oil, oregano essential oil, terpineol, camphor, limonene, and caryophyllene, wherein the mass fraction ratio of wormwood essential oil: eucalyptus essential oil: tea tree essential oil: thyme essential oil: oregano essential oil: terpineol: camphor: limonene: caryophyllene is (80-100): (0-4): (0-3): (0-3): (0-3): (0-3): (0-3): (0-2): (0-1): (0-1).

[0008] The second aspect of the present invention provides a method for preparing the antibacterial moxibustion stick according to the first aspect, comprising the following steps:

[0009] (1) adding antibacterial essential oil to a 0.8%-3% gelatin aqueous solution, emulsifying and dispersing at a rotation speed of 8000-12000 rpm for 4-8 minutes to form an emulsion, adding a 0.1%-0.3% sodium carboxymethyl cellulose solution to the emulsion, stirring at a speed of 800±50 rpm for 15 minutes in a water bath at 35±5°C, adding 10%-15% acetic acid dropwise to adjust the pH value to 4.5-5.8, and obtaining a first solution after round spherical microcapsules are formed as observed under a microscope;

[0010] (2) stirring the first solution obtained in step (1) for 30 minutes, adding a curing agent having a gelatin mass fraction of 1% to 2% and continuing to stir for 2 hours, and adding lime milk dropwise to adjust the pH value to 6.0 to 7.0 to obtain an antibacterial essential oil microcapsule aqueous solution;

[0011] (3) allowing the antibacterial essential oil microcapsule aqueous solution obtained in step (2) to stand for stratification, then discarding the upper aqueous solution, and washing the microcapsule precipitate with 50% ethanol solution and water until the surface oil phase is completely eluted, thereby obtaining the antibacterial essential oil microcapsules;

[0012] (4) diluting the antibacterial essential oil microcapsules described in step (3) with water to obtain an antibacterial essential oil microcapsule solution containing 5% of the mass fraction of the antibacterial essential oil, spraying the solution on natural moxa at a rate of 10%-20% of the mass of natural moxa, and then drying the solution, and rolling the solution into moxa sticks to obtain antibacterial moxibustion sticks.

[0013] Preferably, the curing agent in step (2) is one of alanine aminotransferase, formaldehyde and tea polyphenols, and the amount used is 1:4-1:2 of the amount of gelatin.

[0014] Preferably, in step (1), the mass ratio of the antibacterial essential oil to the gelatin aqueous solution is 2:1-1:2.

[0015] The third aspect of the present invention provides a use of the antibacterial moxibustion strip described in the first aspect for moxibustion. The antibacterial effect of the moxibustion of the present invention is more significant than that of ordinary moxibustion.

[0016] The present invention has the following beneficial effects:

[0017] 1. Natural moxa has certain antibacterial and anti-inflammatory effects, but the preparation process of natural moxa is complicated and the quality is uneven, resulting in the moxibustion sticks prepared from natural moxa not having significant antibacterial and anti-inflammatory effects. The present invention is the first to disperse antibacterial essential oil in the form of microcapsules on the surface of natural moxa to prepare moxibustion sticks for moxibustion. Compared with traditional moxibustion sticks, the antibacterial effect of the antibacterial moxibustion sticks on Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa is more than twice that of traditional moxibustion sticks, and the effect is significant.

[0018] 2. The main reason why the antibacterial effect of directly dispersing the antibacterial essential oil into natural moxa is not significant is that the maximum combustion temperature during moxibustion is 400-600°C. If the moxibustion essential oil is directly sprayed, it will be completely volatilized before the smoke is delivered to the skin. However, when the antibacterial moxibustion strip obtained by the present invention starts to burn, the skin pores at the moxibustion site begin to open. When the combustion temperature reaches the maximum temperature of 400-600°C, the microcapsule skin on the surface of the antibacterial moxa is just broken, and the capsule core material is released and then absorbed by the skin, thereby greatly improving the antibacterial effect.

[0019] 3. The specific ratio of wormwood essential oil, eucalyptus essential oil, tea tree essential oil, thyme essential oil, oregano essential oil, terpineol, camphor, limonene and caryophyllene in the present invention can produce synergistic synergy with natural wormwood, significantly increasing the antibacterial effect of the antibacterial moxibustion strip of the present invention.

[0020] 4. The determined addition amount of sodium carboxymethyl cellulose and gelatin combined with the determined process parameters of the present invention can just ensure that the capsule skin of the microcapsule of the present invention is broken in the range of 400-600°C. At the same time, the present invention needs to use 10%-15% acetic acid to adjust the pH to form round spherical microcapsules, and add 1%-2% curing agent to ensure the stability of the microcapsules. Changes in parameters will change the properties of the reaction system and the shape of the microcapsules, which will result in the failure to obtain the capsule skin of the present invention that breaks at 400-600°C.

[0021] 5. The preparation process of natural moxa is complicated and its quality stability is poor, resulting in the moxa products prepared from natural moxa having a single effect and poor stability. The present invention greatly improves the stability of the antibacterial effect of natural moxa by preparing the antibacterial ingredients into microcapsules and then preparing them into moxa sticks with natural moxa. In addition, the present invention can also supplement other functional ingredients in the microcapsule core according to the efficacy requirements to improve the efficacy range of moxa. DETAILED DESCRIPTION

[0022] The content of the present invention is further explained below through specific implementation examples.

[0023] Example 1

[0024] Antibacterial essential oil 1# was obtained by mixing 850 g of wormwood essential oil, 30 g of eucalyptus essential oil, 30 g of tea tree essential oil, 30 g of thyme essential oil, 30 g of oregano essential oil and 30 g of pineneol.

[0025] Example 2

[0026] A method for preparing an antibacterial moxibustion stick comprises the following steps:

[0027] (1) adding 1 kg of antibacterial essential oil to 100 kg of an aqueous solution containing 0.9% gelatin by mass, emulsifying and dispersing the solution at 12000 rpm for 8 min to form an emulsion, adding a 0.1% sodium carboxymethyl cellulose solution to the emulsion, stirring the solution at 800 rpm for 15 min in a water bath at 35° C., adding 10% acetic acid dropwise to adjust the pH value to 4.5, and obtaining a first solution after round spherical microcapsules are formed as observed under a microscope;

[0028] (2) stirring the first solution obtained in step (1) for 30 minutes, adding 2% gelatin alanine aminotransferase and continuing to stir for 2 hours, and adding lime milk to adjust the pH value to 6.0 to obtain an antibacterial essential oil microcapsule aqueous solution;

[0029] (3) allowing the antibacterial essential oil microcapsule aqueous solution obtained in step (2) to stand for stratification, then discarding the upper aqueous solution, and washing the microcapsule precipitate with 50% ethanol solution and water until the surface oil phase is completely eluted, thereby obtaining the antibacterial essential oil microcapsules;

[0030] (4) diluting the antibacterial essential oil microcapsules described in step (3) with water to obtain an antibacterial essential oil microcapsule solution containing 5% of the mass fraction of the antibacterial essential oil, spraying the solution on natural moxa wool at 20% of the mass of natural moxa wool, and then drying the solution in the air, and rolling the solution into moxa sticks to obtain antibacterial moxibustion stick A#.

[0031] Comparative Example 1

[0032] The only difference between the B# sample in this comparative example and Example 2 is that the antibacterial essential oil microcapsules are replaced by wormwood essential oil sprayed on the surface of natural wormwood; the only difference between the C# sample in this comparative example and Example 2 is that the antibacterial essential oil microcapsules are replaced by water sprayed on the surface of natural wormwood, and the rest are the same.

[0033] After lighting A#, B#, and C# moxibustion sticks to collect smoke (any method of collecting smoke in the prior art can be used), the smoke was extracted with methanol to obtain effective components 1#, 2#, and 3# samples respectively. Three Oxford cups were placed at the center of the surface of three culture media respectively, and 1#, 2#, and 3# moxibustion sticks were introduced into the Oxford cups respectively. Then, 0.2 mL of bacterial solution with a concentration of 1x10 7 CFU / mL of Escherichia coli (spores), Staphylococcus aureus (spores), Bacillus subtilis (spores), and Pseudomonas aeruginosa (spores) suspensions were added to the above-mentioned culture dishes, evenly spread with a spreading rod, and cultured in a 37°C biochemical incubator for 24 hours. After the culture was completed, the diameter of the inhibition zone was measured, and the measurement was repeated 3 times in parallel, and the average value was taken as the experimental result. The experimental results are shown in Table 1 below.

[0034] Table 1 Antibacterial effects of three moxibustion sticks on different bacterial species

[0035]

[0036] Note: Antibacterial diameter (N = 10mm)

[0037] Conclusion: From the results of the antibacterial experiment, it can be seen that the antibacterial effect of the antibacterial moxibustion strip prepared by the present invention is more than twice that of the blank control sample.

[0038] Example 3

[0039] Take 500g of wormwood essential oil to obtain antibacterial essential oil 2#.

[0040] Example 4

[0041] A method for preparing an antibacterial moxibustion stick comprises the following steps:

[0042] (1) adding 500 g of antibacterial essential oil to 500 g of an aqueous solution containing 3% gelatin by mass, emulsifying and dispersing at 9000 rpm for 5 min to form an emulsion, adding a 0.3% sodium carboxymethyl cellulose solution to the emulsion, stirring at 800 rpm for 15 min in a water bath at 35° C., adding 12% acetic acid dropwise to adjust the pH value to 5.0, and obtaining a first solution after round spherical microcapsules are formed as observed under a microscope;

[0043] (2) stirring the first solution obtained in step (1) for 30 minutes, adding formaldehyde with a gelatin mass fraction of 1% and continuing to stir for 2 hours, and adding lime milk dropwise to adjust the pH value to 7.0 to obtain an antibacterial essential oil microcapsule aqueous solution;

[0044] (3) allowing the antibacterial essential oil microcapsule aqueous solution obtained in step (2) to stand for stratification, then discarding the upper aqueous solution, and washing the microcapsule precipitate with 50% ethanol solution and water until the surface oil phase is completely eluted, thereby obtaining the antibacterial essential oil microcapsules;

[0045] (4) diluting the antibacterial essential oil microcapsules in step (3) with water to an antibacterial essential oil microcapsule solution with an antibacterial essential oil mass fraction of 5%, spraying the solution on natural moxa wool at a mass fraction of 10% of natural moxa wool, and then drying the solution, and rolling the solution into moxa sticks to obtain antibacterial moxibustion stick A#

[0046] Comparative Example 2

[0047] The only difference between the B# sample in this comparative example and Example 4 is that the antibacterial essential oil microcapsules are replaced by wormwood essential oil sprayed on the surface of natural wormwood; the only difference between the C# sample in this comparative example and Example 4 is that the antibacterial essential oil microcapsules are replaced by water sprayed on the surface of natural wormwood, and the rest are the same.

[0048] After lighting A#, B#, and C# moxibustion sticks to collect smoke (any method of collecting smoke in the prior art can be used), the smoke is extracted with methanol to obtain effective components 1#, 2#, and 3# samples respectively. Three Oxford cups are placed at the center of the surface of three culture media respectively, and 1#, 2#, and 3# samples are introduced into the Oxford cups respectively. Then, 0.2 mL of bacterial solution with a concentration of 1x10 7 CFU / mL of Escherichia coli (spores), Staphylococcus aureus (spores), Bacillus subtilis (spores), and Pseudomonas aeruginosa (spores) suspensions were added to the above-mentioned culture dishes, evenly spread with a coating rod, and cultured in a 37°C biochemical incubator for 24 hours. After the culture was completed, the diameter of the inhibition zone was measured, and the measurement was repeated 3 times in parallel, and the average value was taken as the experimental result. The experimental results are shown in Table 2 below.

[0049] Table 2 Antibacterial effects of three moxibustion sticks on different bacterial species

[0050]

[0051] Note: Antibacterial diameter (N = 10mm)

[0052] Conclusion: From the results of the antibacterial experiment, it can be seen that the antibacterial effect of the antibacterial moxibustion strip prepared by the present invention is more than twice that of the blank control sample.

[0053] Example 5

[0054] Antibacterial essential oil 3# was obtained by mixing 1750 g of wormwood essential oil, 80 g of eucalyptus essential oil, 40 g of tea tree essential oil, 50 g of oregano essential oil, 40 g of terpineol, 20 g of camphor, 10 g of limonene and 10 g of caryophyllene.

[0055] Example 6

[0056] A method for preparing an antibacterial moxibustion stick comprises the following steps:

[0057] (1) adding 6 kg of antibacterial essential oil to 200 kg of an aqueous solution containing 2% gelatin by mass, emulsifying and dispersing at a speed of 10,000 rpm for 8 min to form an emulsion, adding a sodium carboxymethyl cellulose solution containing 0.2% by mass to the emulsion, stirring at a speed of 800 rpm for 15 min in a water bath at 35° C., adding 15% acetic acid dropwise to adjust the pH value to 5.8, and obtaining a first solution after rounded spherical microcapsules are observed under a microscope;

[0058] (2) stirring the first solution obtained in step (1) for 30 minutes, adding tea polyphenols with a gelatin mass fraction of 1.5%, stirring for 2 hours, and adding lime milk to adjust the pH value to 6.5, thereby obtaining an antibacterial essential oil microcapsule aqueous solution; (3) standing the antibacterial essential oil microcapsule aqueous solution obtained in step (2) for stratification, pouring off the upper layer of water, and washing the microcapsule precipitate with 50% ethanol solution and water until the surface oil phase is completely eluted, thereby obtaining antibacterial essential oil microcapsules;

[0059] (4) diluting the antibacterial essential oil microcapsules in step (3) with water to obtain an antibacterial essential oil microcapsule solution containing 5% of the mass fraction of the antibacterial essential oil, spraying the solution on the natural moxa wool at a mass fraction of 15% of the mass of the natural moxa wool, and then drying the solution, and rolling the solution into a moxa stick to obtain an antibacterial moxibustion stick A#

[0060] Comparative Example 3

[0061] The only difference between the B# sample in this comparative example and Example 6 is that the antibacterial essential oil microcapsules are replaced by wormwood essential oil sprayed on the surface of natural wormwood; the only difference between the C# sample in this comparative example and Example 6 is that the antibacterial essential oil microcapsules are replaced by water sprayed on the surface of natural wormwood, and the rest are the same.

[0062] After lighting A#, B#, and C# moxibustion sticks to collect smoke (any method of collecting smoke in the prior art can be used), the smoke is extracted with methanol to obtain effective components 1#, 2#, and 3# samples respectively. Three Oxford cups are placed at the center of the surface of three culture media respectively, and 1#, 2#, and 3# samples are introduced into the Oxford cups respectively. Then, 0.2 mL of bacterial solution with a concentration of 1x10 7 CFU / mL of Escherichia coli (spores), Staphylococcus aureus (spores), Bacillus subtilis (spores), and Pseudomonas aeruginosa (spores) suspensions were added to the above-mentioned culture dishes, evenly spread with a spreading rod, and cultured in a 37°C biochemical incubator for 24 hours. After the culture was completed, the diameter of the inhibition zone was measured, and the measurement was repeated 3 times in parallel. The average value was taken as the experimental result. The experimental results are shown in Table 3 below.

[0063] Table 3 Antibacterial effects of three moxibustion sticks on different bacterial species

[0064]

[0065] Note: Antibacterial diameter (N = 10mm)

[0066] Conclusion: From the results of the antibacterial experiment, it can be seen that the antibacterial effect of the antibacterial moxibustion strip prepared by the present invention is more than twice that of the blank control sample.

[0067] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. An antibacterial moxibustion stick, It is characterized in that The antibacterial moxibustion stick is rolled with antibacterial moxa, and antibacterial microcapsules are attached to the surface of the antibacterial moxa. When the antibacterial moxibustion stick burns and the temperature reaches 400-600° C., the skin of the antibacterial microcapsule ruptures to release the antibacterial essential oil.

2. The antibacterial moxibustion strip according to claim 1, It is characterized in that The antibacterial essential oil includes wormwood essential oil, eucalyptus essential oil, tea tree essential oil, thyme essential oil, oregano essential oil, terpineol, camphor, limonene, and caryophyllene, wherein the mass fraction ratio of wormwood essential oil: eucalyptus essential oil: tea tree essential oil: thyme essential oil: oregano essential oil: terpineol: camphor: limonene: caryophyllene is (80-100): (0-4): (0-3): (0-3): (0-3): (0-3): (0-3): (0-2): (0-1): (0-1).

3. A method for preparing the antibacterial moxibustion strip according to any one of claims 1 to 2, It is characterized in that The following steps are involved: (1) adding antibacterial essential oil to a 0.8%-3% gelatin aqueous solution, emulsifying and dispersing at a rotation speed of 8000-12000 rpm for 4-8 minutes to form an emulsion, adding a 0.1%-0.3% sodium carboxymethyl cellulose solution to the emulsion, stirring at a speed of 800±50 rpm for 15 minutes in a water bath at 35±5°C, adding 10%-15% acetic acid dropwise to adjust the pH value to 4.5-5.8, and obtaining a first solution after round spherical microcapsules are formed as observed under a microscope; (2) stirring the first solution obtained in step (1) for 30 minutes, adding a curing agent having a gelatin mass fraction of 1% to 2% and continuing to stir for 2 hours, and adding lime milk dropwise to adjust the pH value to 6.0 to 7.0 to obtain an antibacterial essential oil microcapsule aqueous solution; (3) allowing the antibacterial essential oil microcapsule aqueous solution obtained in step (2) to stand for stratification, then discarding the upper aqueous solution, and washing the microcapsule precipitate with 50% ethanol solution and water until the surface oil phase is completely eluted, thereby obtaining the antibacterial essential oil microcapsules; (4) diluting the antibacterial essential oil microcapsules described in step (3) with water to obtain an antibacterial essential oil microcapsule solution containing 5% of the mass fraction of the antibacterial essential oil, spraying the solution on natural moxa at a rate of 10%-20% of the mass of natural moxa, and then drying the solution, and rolling the solution into moxa sticks to obtain antibacterial moxibustion sticks.

4. The preparation method according to claim 3, It is characterized in that The curing agent described in step (2) is one of alanine aminotransferase, formaldehyde and tea polyphenols, and the amount used is 1:4-1:2 of the amount of gelatin.

5. The preparation method according to claim 3, It is characterized in that In the step (1), the mass ratio of the antibacterial essential oil to the gelatin aqueous solution is 2:1-1:

2.

6. A use of the antibacterial moxibustion strip according to any one of claims 1 to 2, It is characterized in that The antibacterial moxibustion strip is used for moxibustion.

Images