Single molecule immunoassay analyzer

Abstract

The invention provides a single molecule immunity analyzer, and relates to the technical field of single molecule analys.The single molecule immunity analyzer comprises a shell assembly and a bottom plate assembly, the upper end of the bottom plate assembly is sleeved with the shell assembly, and the upper end of the shell assembly is provided with a movement assembly, a mixing movement assembly, a movable pipette assembly, a sampling liquid path assembly and an optical detection assembly; the moving assembly is located at the side end part of the bottom plate assembly, the uniform mixing moving assembly is located at the upper end part of the moving assembly, the moving row gun assembly is located at the upper end part of the uniform mixing moving assembly, and the sampling liquid path assembly is located at the upper end part of the bottom plate assembly. According to the invention, the structure of each system is simplified, the function integration is realized, the detection efficiency is improved, meanwhile, instruments are reasonably arranged, the height space is fully utilized, the overall occupied volume is reduced, sampling, washing and detection systems are optimized, and the precision, safety and reliability of the detection structure are greatly improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of single molecule analysis, and more specifically, particularly relates to a single molecule immunoassay analyzer. Background Art

[0002] The single-molecule immunoassay method restricts the molecules to be detected to an extremely small spatial range (below nanoliters) and performs absolute counting of the detection signals. It can detect proteins in body fluids such as serum, plasma, and tears with ultra-high sensitivity (up to 10-18 mol / L), and has a wide range of applications in clinical and scientific research.

[0003] Existing single-molecule immunoassay devices have the following disadvantages:

[0004] 1. The existing testing platforms have the disadvantages of high processing precision and high production costs. In addition, the existing testing platforms take more than one hour for a single test, which is quite time-consuming;

[0005] 2. Existing optical inspection modules are mostly built with multiple scattered parts. Since there may be errors in the assembly of parts and there may be debugging differences during each debugging process, it is not conducive to system debugging and modular production.

[0006] Therefore, in view of this, the existing structure and defects are studied and improved, and a single-molecule immunoassay analyzer is provided, in order to achieve a more practical purpose. Summary of the invention

[0007] In order to solve the above technical problems, the present invention provides a single molecule immunoassay analyzer to solve the above problems.

[0008] A single-molecule immunoassay analyzer comprises a shell assembly and a bottom plate assembly, wherein the shell assembly is sleeved on the upper end of the bottom plate assembly, and the upper end of the shell assembly is provided with a motion assembly, a mixing and moving assembly, a mobile gun assembly, a sampling liquid path assembly and an optical detection assembly, wherein the motion assembly is located at the side end of the bottom plate assembly, the mixing and moving assembly is located at the upper end of the motion assembly, the mobile gun assembly is located at the upper end of the mixing and moving assembly, the sampling liquid path assembly is located at the upper end of the bottom plate assembly, and the optical detection assembly is located at the upper end of the sampling liquid path assembly, and the bottom plate assembly is integrated with a motion platform driving screw, a first bottom plate, a power supply assembly and a power supply interface, the motion platform driving screw is fixedly mounted on the upper end of the first bottom plate, the power supply assembly is fixedly mounted on the upper end of the first bottom plate, and the power supply interface is fixedly mounted on the upper end of the first bottom plate, and the motion assembly is integrated with a reagent box rack assembly and a magnetic rack assembly , a moving plate assembly and a Tip head assembly, the magnetic frame assembly is fixedly mounted on the lower end of the test kit rack assembly, the moving plate assembly is fixedly mounted on the lower end of the magnetic frame assembly, the Tip head assembly is fixedly mounted on the side end of the test kit rack assembly, the mixing moving assembly is integrated with a supporting platform, a first stepper motor, a slide rail and a mixing module, the first stepper motor is fixedly mounted on the upper end of the supporting platform, the slide rail is fixedly mounted on the upper end of the supporting platform, the mixing module is slidably mounted on the slide rail, the mobile gun assembly is integrated with a gun horizontal plate, a gun bracket and a second stepper motor, the gun bracket is fixedly mounted on the side end of the gun horizontal plate, the second stepper motor is fixedly mounted on the upper end of the gun horizontal plate, the suction and discharge assembly is fixedly mounted on the upper end of the gun horizontal plate, the second stepper motor is located at both side ends of the suction and discharge assembly, and a plurality of Ti The tip head ejection plate is a tip head ejection plate, and the lower end of each tip head ejection plate is fixedly installed with a tip head body, the sampling liquid path component is integrated with a second base plate, a horizontal movable slide rail and a third stepper motor, the horizontal movable slide rail is fixedly installed on the upper end of the second base plate, and the third stepper motor is fixedly installed on the lower end of the second base plate, the sampling needle vertical moving component is slidably installed on the horizontal movable slide rail, and the lower end of the sampling needle vertical moving component is fixedly installed with a sampling needle, and the optical detection component is integrated with an optical path base plate, a laser, a PMT, an APD fixing plate, a laser driver and an optical path control board.

[0009] Preferably, the laser is fixedly mounted on the upper end of the optical path base plate, an attenuation plate is fixedly mounted on the side end of the laser, a first objective lens is fixedly mounted on the side end of the attenuation plate, a sheath flow cell is fixedly mounted on the side end of the first objective lens, a second objective lens is fixedly mounted on the side end of the sheath flow cell, and the PMT is fixedly mounted on the upper end of the optical path base plate.

[0010] Preferably, a lens assembly is fixedly installed on the side end of the PMT, the APD fixing plate is fixedly installed on the side end of the optical path base plate, a heat sink is fixedly installed on the upper end of the PMT, a fan is fixedly installed on the upper end of the heat sink, a PMT high-voltage module is fixedly installed on the upper end of the optical path base plate, the laser driver is fixedly installed on the upper end of the optical path base plate, the optical path control board is fixedly installed on the upper end of the optical path base plate, a power adapter is fixedly installed on the upper end of the optical path base plate, and a transformer is also fixedly installed on the upper end of the optical path base plate.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] In the present invention, by providing a bottom plate assembly, a motion assembly, a mixing and moving assembly, a moving discharge gun assembly and a sampling liquid path assembly, the motion platform drives the lead screw and the third stepping motor to jointly drive the precise movement of the sampling needle and the liquid transfer discharge gun, thereby achieving precise sampling, liquid distribution and mixing operations. Such high-precision sample processing and liquid operation are crucial in single-molecule immunoassay, helping to reduce experimental errors and improve the reproducibility of test results.

[0013] In the present invention, an optical detection component, a laser, a PMT, and a laser driver are provided. The optical detection component emits a light beam through a high-precision laser and focuses the light through an objective lens, and the PMT amplifies the weak light signal. This design improves the sensitivity of the detection system, enabling it to detect and amplify signals at the single molecule level, thereby achieving extremely high sensitivity detection.

[0014] In the present invention, by streamlining the structure of each system, functional integration is achieved, detection efficiency is improved, and at the same time, the instruments are reasonably arranged, the height space is fully utilized, the overall occupied volume is reduced, and the sample addition, washing and detection systems are optimized, thereby greatly improving the accuracy of the detection structure and safety and reliability.

[0015] In the present invention, by limiting the molecules to be detected within an extremely small spatial range (below nanoliters) and performing absolute counting of the detection signals, ultra-high sensitivity detection of proteins in body fluids such as serum, plasma, and tears can be performed, thereby achieving high-sensitivity detection of low-abundance samples.

[0016] In the present invention, a gun row horizontal plate and a gun row bracket are provided to allow multi-channel parallel pipetting operations, and the magnetic frame assembly and the Tip head assembly further enhance the ability to process multiple samples simultaneously. This multi-channel design greatly improves the efficiency of sample processing and shortens the total experimental time of single molecule immunoassay.

[0017] In the present invention, the modular design makes the system configuration flexible and easy to maintain. Both the adjustment of the optical detection part and the maintenance of the liquid processing part can be completed quickly, which reduces downtime and improves the working efficiency and adaptability of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of the housing assembly of the present invention;

[0019] Figure 2 It is a schematic diagram of the structure of the bottom plate assembly of the present invention;

[0020] Figure 3 It is a schematic diagram of the structure of the power supply assembly of the present invention;

[0021] Figure 4 It is a schematic diagram of the structure of the motion component of the present invention;

[0022] Figure 5 It is a schematic diagram of the structure of the mixing and moving assembly of the present invention;

[0023] Figure 6 It is a schematic diagram of the Tip head structure of the present invention;

[0024] Figure 7 It is a schematic diagram of the structure of the sampling liquid path component of the present invention;

[0025] Figure 8 It is a schematic diagram of the structure of the optical path base plate of the present invention.

[0026] In the figure, the corresponding relationship between the component names and the figure numbers is: 100, shell assembly; 200, bottom plate assembly; 300, motion assembly; 400, mixing and moving assembly; 500, mobile row gun assembly; 600, sampling liquid path assembly; 700, optical detection assembly; 201, motion platform driving screw; 202, first bottom plate; 203, power supply assembly; 204, power supply interface; 310, reagent box rack assembly; 320, magnetic rack assembly; 330, motion plate assembly; 340, Tip head assembly; 401, support platform; 402, first stepper motor; 403, slide rail; 404, mixing module; 501, row gun horizontal plate; 502, row gun bracket; 503, Tip head body; 504, Tip head body; 505, Tip head body; 506, Tip head body; 507, Tip head body; 508, Tip head body; 509, Tip head body; 510, Tip head body; 511, Tip head body; 512, Tip head body; 513, Tip head body; 514, Tip head body; 515, Tip head body; 516, Tip head body; 517, Tip head body; 518, Tip head body; 519, Tip head body; 520, Tip head body; 521, Tip head body; 522, Tip head body; 523, Tip head body; 524, Tip head body; 525, Tip head body; 526, Tip head body; 527, Tip head body; 528, Tip head body; 529, Tip head body; 530, Tip head body; 531 IP head ejection plate; 505, suction and discharge assembly; 506, second stepper motor; 601, second base plate; 602, horizontal moving slide rail; 603, third stepper motor; 620, sampling needle vertical moving assembly; 621, sampling needle; 701, optical path base plate; 702, laser; 703, attenuation plate; 704, first objective lens; 705, second objective lens; 706, lens assembly; 707, PMT; 708, APD fixing plate; 709, fan; 710, heat sink; 711, PMT high voltage module; 712, laser driver; 713, optical path control board; 714, power adapter; 715, sheath flow pool; 716, transformer. DETAILED DESCRIPTION

[0027] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0028] See also Figure 1 - Figure 8The present invention provides a single molecule immunoassay analyzer, comprising a housing component 100 and a bottom plate component 200, wherein the housing component 100 is sleeved on the upper end of the bottom plate component 200, and the upper end of the housing component 100 is provided with a motion component 300, a mixing and moving component 400, a mobile discharge gun component 500, a sampling liquid path component 600 and an optical detection component 700, wherein the motion component 300 is located at the side end of the bottom plate component 200, the mixing and moving component 400 is located at the upper end of the motion component 300, the mobile discharge gun component 500 is located at the upper end of the mixing and moving component 400, the sampling liquid path component 600 is located at the upper end of the bottom plate component 200, and the optical detection component 700 is located at the upper end of the sampling liquid path component 600, and the bottom plate component 200 is integrated with a motion platform driving screw 201, a first Base plate 202, power supply assembly 203 and power supply interface 204, the motion platform driving screw 201 is fixedly installed on the upper end of the first base plate 202, the power supply assembly 203 is fixedly installed on the upper end of the first base plate 202, and the power supply interface 204 is fixedly installed on the upper end of the first base plate 202. In the base plate assembly 200, the first base plate 202 is the basic structure of the entire base plate assembly, and its main function is to provide stable installation and support for other components. The motion platform driving screw 201 is a linear transmission device for driving and controlling the motion platform. The power supply assembly 203 provides power for the entire analyzer, and is responsible for converting the external power supply into the working voltage and current required by each component, and providing a stable power supply. The power supply interface 204 is the access port for connecting the analyzer with the external power supply;

[0029] The motion component 300 is integrated with a reagent box rack component 310, a magnetic rack component 320, a motion plate component 330 and a Tip head component 340. The magnetic rack component 320 is fixedly mounted on the lower end of the reagent box rack component 310, the motion plate component 330 is fixedly mounted on the lower end of the magnetic rack component 320, and the Tip head component 340 is fixedly mounted on the side end of the reagent box rack component 310. The reagent box rack component 310 is used to fix and store the reagent box used in the analysis process. It ensures that the reagent box is accurately positioned inside the instrument, which facilitates the motion component to perform precise pipetting operations and sample processing. The magnetic rack component 320 is used to separate magnetic particles in the sample, which is usually used for sample purification or magnetic marker processing. The magnetic rack provides a stable magnetic field to absorb magnetic particles, thereby separating them from the liquid part. The motion plate component 330 is the core part of the entire motion system, responsible for carrying and moving different sample or reagent positions. The Tip head component 340 is a key component in the pipetting operation, responsible for installing and replacing the Tip head body 503, and performing the absorption and distribution of samples or reagents. The high-precision motion plate assembly 330 ensures the accuracy and repeatability of the pipetting process;

[0030] The mixing moving component 400 is integrated with a support platform 401, a first stepper motor 402, a slide rail 403 and a mixing module 404. The first stepper motor 402 is fixedly installed on the upper end of the support platform 401, the slide rail 403 is fixedly installed on the upper end of the support platform 401, and the mixing module 404 is slidably installed on the slide rail 403. The support platform 401 provides a stable foundation for the mixing moving component 400 for installing and supporting other components, such as the mixing module 404 and the first stepper motor 402. It ensures the smooth operation of the entire mixing process and effectively absorbs and reduces the vibration generated during mixing. The first stepper motor 402 is the driving core of the mixing moving component 400. The center is responsible for controlling the precise movement and position adjustment of the mixing module 404. It realizes precise control of the slide rail 403 and the mixing module 404 through step-by-step rotation to ensure the accuracy and consistency of the mixing operation. The slide rail 403 provides a linear motion path for the mixing module 404 to ensure that the mixing module 404 can move smoothly during the mixing process. The accuracy and lubrication condition of the slide rail 403 directly affect the stability and efficiency of the mixing operation. The mixing module 404 is the core component that actually performs the mixing operation. It mixes the sample or reagent through vibration or rotation. The mixing module 404 is usually optimized according to the required mixing mode to ensure that the sample is evenly mixed and improve the effectiveness of the reaction;

[0031] The mobile gun assembly 500 integrates a gun horizontal plate 501, a gun bracket 502 and a second stepper motor 506. The gun bracket 502 is fixedly mounted on the side end of the gun horizontal plate 501, and the second stepper motor 506 is fixedly mounted on the upper end of the gun horizontal plate 501. The suction and discharge assembly 505 is fixedly mounted on the upper end of the gun horizontal plate 501. The second stepper motor 506 is located at the two side ends of the suction and discharge assembly 505. A plurality of tip head ejection plates 504 are fixedly mounted on the lower end of the gun bracket 502. Each tip head ejection plate 504 is fixedly mounted on the lower end of the gun bracket 502. The lower end of the ip head ejection plate 504 is fixedly installed with a Tip head body 503. The gun cross plate 501 is the main structural support of the mobile gun assembly 500, which is used to connect and fix multiple pipette guns. It ensures that the pipette guns maintain a fixed spacing and stability during operation, thereby achieving precise operation of multi-channel simultaneous pipetting. The gun bracket 502 is used to fix the pipette gun and ensure its stability during operation. It provides a safe connection point so that the pipette gun can accurately perform aspiration and discharge operations, and the gun can be replaced or adjusted when necessary. The Tip head body 503 is the actual operating end of the pipette gun, which is used to absorb and dispense liquids. Each Tip head body 503 is connected to the corresponding channel of the pipette gun, which can accurately control the volume and speed of each pipetting. The Tip head push plate 504 is used to safely and quickly remove the Tip head body 503 after the pipetting operation is completed. It pushes the used Tip head body 503 from the gun through an electric pushing mechanism, which is convenient for replacing a new Tip head body 503, ensuring the continuity and operation efficiency of the pipetting process. The suction and discharge component 505 is the core operating component of the pipette gun, which is responsible for controlling the suction and discharge of liquid. It usually includes a pneumatic or electric driving mechanism, and realizes accurate adjustment of the liquid volume through precise pressure control. The second stepper motor 506 is responsible for driving the movement of the gun cross plate 501 and controlling the working position of the suction and discharge component 505. Through precise stepping control, the second stepper motor 506 can ensure that the pipette gun is accurately positioned during operation, thereby improving the accuracy and consistency of the operation;

[0032] The sampling liquid path assembly 600 integrates a second base plate 601, a horizontal movable slide rail 602 and a third stepper motor 603. The horizontal movable slide rail 602 is fixedly mounted on the upper end of the second base plate 601, and the third stepper motor 603 is fixedly mounted on the lower end of the second base plate 601. The sampling needle vertical movement assembly 620 is slidably mounted on the horizontal movable slide rail 602. The lower end of the sampling needle vertical movement assembly 620 is fixedly mounted with a sampling needle 621. The second base plate 601 is the basic structure of the entire sampling liquid path assembly 600, which is responsible for supporting and fixing all other components. The horizontal movable slide rail 602 provides a path for the horizontal movement of the sampling needle 621, so that the sampling needle 621 can move between multiple samples. The third stepper motor 603 is responsible for driving the horizontal movement of the sampling needle 621 and the lifting and lowering of the vertical movement assembly. The sampling needle vertical movement assembly 62 0 is responsible for controlling the up and down movement of the sampling needle 621 so that it can be accurately inserted into the sample container for sampling or liquid separation. The sampling needle 621 is a component that directly contacts the sample and is used to absorb or distribute liquid from the sample container. The second bottom plate 601 is the basic structure of the entire sampling liquid path assembly 600 and is responsible for supporting and fixing all other components. The horizontal moving slide rail 602 provides a path for the horizontal movement of the sampling needle 621 so that the sampling needle 621 can move between multiple samples. The third stepper motor 603 is responsible for driving the horizontal movement of the sampling needle 621 and the lifting and lowering of the vertical moving assembly. The sampling needle vertical moving assembly 620 is responsible for controlling the up and down movement of the sampling needle 621 so that it can be accurately inserted into the sample container for sampling or liquid separation. The sampling needle 621 is a component that directly contacts the sample and is used to absorb or distribute liquid from the sample container.

[0033] The optical detection component 700 integrates an optical path base plate 701, a laser 702, a PMT 707, an APD fixing plate 708, a laser driver 712 and an optical path control plate 713. The laser 702 is fixedly mounted on the upper end of the optical path base plate 701, an attenuation plate 703 is fixedly mounted on the side end of the laser 702, a first objective lens 704 is fixedly mounted on the side end of the attenuation plate 703, a sheath flow cell 715 is fixedly mounted on the side end of the first objective lens 704, and a second The objective lens 705 and the PMT 707 are fixedly mounted on the upper end of the optical path bottom plate 701. The side end of the PMT 707 is fixedly mounted with a lens assembly 706. The APD fixing plate 708 is fixedly mounted on the side end of the optical path bottom plate 701. The upper end of the PMT 707 is fixedly mounted with a heat sink 710. The upper end of the heat sink 710 is fixedly mounted with a fan 709. The upper end of the optical path bottom plate 701 is fixedly mounted with a PMT high-voltage module 711. The laser driver 712 is fixedly mounted on the upper end of the optical path bottom plate 701. , the optical path control board 713 is fixedly mounted on the upper end of the optical path base plate 701, a power adapter 714 is fixedly mounted on the upper end of the optical path base plate 701, and a transformer 716 is also fixedly mounted on the upper end of the optical path base plate 701. The optical path base plate 701 is the basis of the entire optical detection assembly 700, providing stable support and fixing all optical components. The laser 702 is the light source for optical detection, emitting a high-intensity and precise laser beam for irradiating samples or passing through optical systems. The attenuation plate 703 is used to adjust the intensity of the laser beam, and protects sensitive detection devices or adjusts the intensity of optical signals by reducing the intensity of the light passing through. The first objective lens 704 and the second objective lens 705 are used to focus the light beam onto the sample or detector to enhance the resolution and sensitivity of the optical system. The lens assembly 706 is used to focus or image the light beam, and usually includes a group of lenses for optimizing the light beam path and improving the resolution of the optical system. The PMT707 is a core device for detecting weak light signals, converting light signals into electrical signals through the photoelectric effect, and amplifying the signals through the multiplication process.It plays a key role in high-sensitivity detection. The APD fixing plate 708 is used to fix the avalanche photodiode (APD) to ensure that it maintains a stable optical path position during the detection process. The fan 709 is used to dissipate heat for the electronic equipment in the optical detection component to prevent overheating from affecting the performance and life of the equipment. The heat sink 710 works together with the fan 709 to further increase the heat dissipation area of ​​the equipment and dissipate heat into the air through heat conduction. The PMT high-voltage module 711 provides the required high-voltage power supply for the PMT707 to ensure its normal operation. The laser driver 712 provides power and control signals for the laser 702 to control the laser The switch, power and mode of the optical detection system, the optical path control board 713 is responsible for controlling and adjusting various optical components in the optical detection system, such as the laser 702, the attenuation plate 703, the lens assembly 706, etc., to ensure the precise adjustment and switching of the optical path, the power adapter 714 provides a stable power supply for various electronic devices in the optical detection assembly 700 to ensure the normal operation of the equipment, the sheath flow cell 715 is used to guide the sample to flow through the detection optical path to ensure the stability and uniformity of the sample flow, and the transformer 716 is used to convert the input voltage into a voltage suitable for the requirements of various components of the optical detection system to ensure that different devices can operate safely and stably.

[0034] Working principle:

[0035] In the first step, when the device is in use, a reagent kit sample area is provided for placing the motion component 300. The area is divided into a TIP area for a discharge gun, a sample storage area, a magnetic bead reagent storage area, a sheath fluid storage area, a reagent reaction area, etc. The single arm, the TIP for a discharge gun and the puncture TIP are pre-packaged in the consumable box. The single arm can sequentially transfer the TIP IP adapter, move the reaction position to get the liquid to the detection pool, the sheath flow reagent is stored in the reagent bottle and is taken by the pump station injection pump. The mobile gun assembly 500 area mainly realizes liquid transfer through the gun bracket 502 and the suction and discharge assembly 505, which mainly includes samples, magnetic beads, fluorescent microspheres, cleaning liquid, transfer liquid, etc. The main function of the single-arm pipetting is to place the transfer liquid in the detection circulation pool. The sampling liquid path assembly 600 area is the liquid processing area for the reagent reaction and detection implementation process, including single-arm pipetting, cleaning waste liquid, and sheath liquid. The optical detection assembly 700 area is the sheath flow pool 715 and the optical detection area. The sheath flow pool 715 separates the magnetic beads, and the number of microfluidic magnetic beads and the intensity of the fluorescent signal are detected through the microfluidic optical detection.

[0036] The second step, in the base plate assembly 200, the first base plate 202 is the basic structure of the entire base plate assembly, and its main function is to provide stable installation and support for other components. The motion platform drive screw 201 is a linear transmission device for driving and controlling the motion platform. The power supply assembly 203 provides power for the entire analyzer, responsible for converting the external power supply into the working voltage and current required by each component, and providing a stable power supply. The power supply interface 204 is the access port for connecting the analyzer with the external power supply, responsible for the transmission of power input, and the reagent box rack assembly 310 is used to fix and store the reagent box used in the analysis process. It ensures that the reagent box is accurately positioned inside the instrument, which facilitates the motion assembly to perform precise pipetting operations and sample processing. The magnetic rack assembly 320 is used to separate magnetic particles in the sample, which is usually used for sample purification or the processing of magnetic markers. The magnetic rack provides a stable magnetic field to absorb magnetic particles, thereby separating them from the liquid part. The moving plate assembly 330 is the core part of the entire motion system, responsible for carrying and moving different samples or reagent positions. The tip head assembly 340 is a key component in the pipetting operation, responsible for installing and replacing the tip head body 503, and performing the aspiration and distribution of samples or reagents. The high-precision moving plate assembly 330 ensures the accuracy and repeatability of the pipetting process.

[0037] The third step is that the support platform 401 provides a stable foundation for the mixing moving component 400 for installing and supporting other components, such as the mixing module 404 and the first stepper motor 402, which ensures the smooth operation of the entire mixing process and effectively absorbs and reduces the vibration generated during mixing. The first stepper motor 402 is the driving core of the mixing moving component 400, responsible for controlling the precise movement and position adjustment of the mixing module 404. It realizes precise control of the slide rail 403 and the mixing module 404 through step-by-step rotation to ensure the accuracy and consistency of the mixing operation. The slide rail 403 provides a linear motion path for the mixing module 404 to ensure that the mixing module 404 can move smoothly during the mixing process. The accuracy and lubrication condition of the slide rail 403 directly affect the stability and efficiency of the mixing operation. The mixing module 404 is the core component for actually performing the mixing operation. It mixes the sample or reagent through vibration or rotation. The mixing module 404 is usually optimized according to the required mixing mode to ensure that the sample is evenly mixed and improve the effectiveness of the reaction.

[0038] The fourth step, the gun cross plate 501 is the main structural support of the mobile gun assembly 500, which is used to connect and fix multiple pipette guns. It ensures that the pipette guns maintain a fixed spacing and stability during operation, thereby achieving precise operation of multi-channel simultaneous pipetting. The gun bracket 502 is used to fix the pipette gun and ensure its stability during operation. It provides a safe connection point so that the pipette gun can accurately perform aspiration and discharge operations, and the gun can be replaced or adjusted when necessary. The Tip head 503 is the actual operating end of the pipette gun, which is used to aspirate and dispense liquids. Each Tip head 503 is connected to the corresponding channel of the pipette gun, which can accurately control the volume and speed of each pipetting. The Tip head push plate 504 is used to safely and quickly remove the Tip head 503 after the pipetting operation is completed. It pushes the used Tip head 503 from the gun through an electric pushing mechanism, which is convenient for replacing a new Tip head 503, ensuring the continuity and operation efficiency of the pipetting process. The suction and discharge component 505 is the core operating component of the pipette gun, which is responsible for controlling the suction and discharge of liquid. It usually includes a pneumatic or electric driving mechanism, and realizes accurate adjustment of the liquid volume through precise pressure control. The second stepper motor 506 is responsible for driving the movement of the gun cross plate 501 and controlling the working position of the suction and discharge component 505. Through precise stepping control, the second stepper motor 506 can ensure that the pipette gun is accurately positioned during operation, thereby improving the accuracy and consistency of the operation.

[0039] Step 5. The second base plate 601 is the basic structure of the entire sampling liquid path assembly 600, responsible for supporting and fixing all other components. The horizontal moving slide rail 602 provides a path for the horizontal movement of the sampling needle 621, so that the sampling needle 621 can move between multiple samples. The third stepper motor 603 is responsible for driving the horizontal movement of the sampling needle 621 and the lifting and lowering of the vertical moving assembly. The sampling needle vertical moving assembly 620 is responsible for controlling the up and down movement of the sampling needle 621, so that it can be accurately inserted into the sample container for sampling or liquid separation. The sampling needle 621 is a component that directly contacts the sample and is used to draw liquid from the sample container or distribute liquid. The optical path base plate 701 is the foundation of the entire optical detection assembly 700 and provides stable support. and fix all optical components. The laser 702 is the light source for optical detection. It emits a high-intensity and precise laser beam for irradiating samples or passing through optical systems. The attenuation plate 703 is used to adjust the intensity of the laser beam. It protects sensitive detection devices or adjusts the intensity of optical signals by reducing the intensity of light passing through. The first objective lens 704 and the second objective lens 705 are used to focus the light beam onto the sample or detector to enhance the resolution and sensitivity of the optical system. The lens assembly 706 is used to focus or image the light beam, and usually includes a group of lenses for optimizing the beam path and improving the resolution of the optical system. The PMT707 is the core device for detecting weak light signals. It converts light signals into electrical signals through the photoelectric effect and amplifies the signals through the multiplication process. It plays a key role in high-sensitivity detection. The APD fixing plate 708 is used to fix the avalanche photodiode (APD) to ensure that it maintains a stable optical path position during the detection process. The fan 709 is used to dissipate heat for the electronic equipment in the optical detection component to prevent overheating from affecting the performance and life of the equipment. The heat sink 710 works together with the fan 709 to further increase the heat dissipation area of ​​the equipment and dissipate heat into the air through heat conduction. The PMT high-voltage module 711 provides the required high-voltage power supply for the PMT707 to ensure its normal operation. The laser driver 712 provides power and control signals for the laser 702 to control the laser The switch, power and mode of the optical detection system, the optical path control board 713 is responsible for controlling and adjusting various optical components in the optical detection system, such as the laser 702, the attenuation plate 703, the lens assembly 706, etc., to ensure the precise adjustment and switching of the optical path, the power adapter 714 provides a stable power supply for various electronic devices in the optical detection assembly 700 to ensure the normal operation of the equipment, the sheath flow cell 715 is used to guide the sample to flow through the detection optical path to ensure the stability and uniformity of the sample flow, and the transformer 716 is used to convert the input voltage into a voltage suitable for the requirements of various components of the optical detection system to ensure that different devices can operate safely and stably.

[0040] The embodiments of the present invention are given for the purpose of illustration and description, and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

Claims

1. A single molecule immunoassay analyzer, comprising a housing assembly (100) and a base assembly (200), characterized in that: The housing component (100) is sleeved on the upper end of the bottom plate component (200), and the upper end of the housing component (100) is provided with a motion component (300), a mixing and moving component (400), a moving discharge gun component (500), a sampling liquid path component (600) and an optical detection component (700). The motion component (300) is located at the side end of the base plate component (200), the mixing and moving component (400) is located at the upper end of the motion component (300), the mobile discharge gun component (500) is located at the upper end of the mixing and moving component (400), the sampling liquid path component (600) is located at the upper end of the base plate component (200), and the optical detection component (700) is located at the upper end of the sampling liquid path component (600).

2. The single molecule immunoassay analyzer according to claim 1, characterized in that: The base plate assembly (200) is integrated with a motion platform driving screw (201), a first base plate (202), a power supply assembly (203) and a power supply interface (204); the motion platform driving screw (201) is fixedly mounted on the upper end of the first base plate (202); the power supply assembly (203) is fixedly mounted on the upper end of the first base plate (202); and the power supply interface (204) is fixedly mounted on the upper end of the first base plate (202).

3. The single molecule immunoassay analyzer according to claim 2, characterized in that: The moving component (300) is integrated with a reagent box rack component (310), a magnetic rack component (320), a moving plate component (330) and a tip head component (340); the magnetic rack component (320) is fixedly mounted on the lower end of the reagent box rack component (310); the moving plate component (330) is fixedly mounted on the lower end of the magnetic rack component (320); and the tip head component (340) is fixedly mounted on the side end of the reagent box rack component (310).

4. The single molecule immunoassay analyzer according to claim 3, characterized in that: The mixing moving assembly (400) is integrated with a support platform (401), a first stepper motor (402), a slide rail (403) and a mixing module (404), wherein the first stepper motor (402) is fixedly mounted on the upper end of the support platform (401), and the slide rail (403) is fixedly mounted on the upper end of the support platform (401); Wherein, the mixing module (404) is slidably mounted on the slide rail (403).

5. The single molecule immunoassay analyzer according to claim 4, characterized in that: The mobile gun assembly (500) integrates a gun row horizontal plate (501), a gun row bracket (502), a suction and discharge assembly (505) and a second stepper motor (506); the gun row bracket (502) is fixedly mounted on the side end of the gun row horizontal plate (501); the second stepper motor (506) is fixedly mounted on the upper end of the gun row horizontal plate (501); and the suction and discharge assembly (505) is fixedly mounted on the upper end of the gun row horizontal plate (501).

6. The single molecule immunoassay analyzer according to claim 5, characterized in that: The second stepper motor (506) is located at the two side ends of the suction and discharge assembly (505), and a plurality of tip head ejection plates (504) are fixedly installed at the lower end of the ejection gun bracket (502), and a tip head body (503) is fixedly installed at the lower end of each tip head ejection plate (504).

7. The single molecule immunoassay analyzer according to claim 6, characterized in that: The sampling liquid path assembly (600) is integrated with a second base plate (601), a horizontal movable slide rail (602), a sampling needle vertical moving assembly (620) and a third stepper motor (603), wherein the horizontal movable slide rail (602) is fixedly mounted on the upper end of the second base plate (601), and the third stepper motor (603) is fixedly mounted on the lower end of the second base plate (601).

8. The single molecule immunoassay analyzer according to claim 7, characterized in that: The sampling needle vertical moving assembly (620) is slidably mounted on a horizontal moving slide rail (602); a sampling needle (621) is fixedly mounted on the lower end of the sampling needle vertical moving assembly (620); and an optical path base plate (701), a laser (702), a PMT (707), an APD fixing plate (708), a laser driver (712) and an optical path control plate (713) are integrated on the optical detection assembly (700).

9. The single molecule immunoassay analyzer according to claim 8, characterized in that: The laser (702) is fixedly mounted on the upper end of the optical path bottom plate (701), an attenuation plate (703) is fixedly mounted on the side end of the laser (702), and a first objective lens (704) is fixedly mounted on the side end of the attenuation plate (703); A sheath flow cell (715) is fixedly mounted on the side end of the first objective lens (704), a second objective lens (705) is fixedly mounted on the side end of the sheath flow cell (715), and the PMT (707) is fixedly mounted on the upper end of the optical path base plate (701).

10. The single molecule immunoassay analyzer according to claim 9, characterized in that: A lens assembly (706) is fixedly mounted on a side end of the PMT (707), the APD fixing plate (708) is fixedly mounted on a side end of the optical path base plate (701), a heat sink (710) is fixedly mounted on an upper end of the PMT (707), a fan (709) is fixedly mounted on an upper end of the heat sink (710), and a PMT high-voltage module (711) is fixedly mounted on an upper end of the optical path base plate (701); The laser driver (712) is fixedly mounted on the upper end of the optical path base plate (701), the optical path control board (713) is fixedly mounted on the upper end of the optical path base plate (701), a power adapter (714) is fixedly mounted on the upper end of the optical path base plate (701), and a transformer (716) is also fixedly mounted on the upper end of the optical path base plate (701).

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