Eluting device for automatic nucleic acid extraction

Abstract

The invention relates to an elution device for automatic nucleic acid extraction, which comprises an elution turntable, a rotating gear capable of rotating is arranged on the elution turntable, a fixed gear is annularly arranged on the periphery of the rotating gear, and a plurality of site gears are arranged between the rotating gear and the fixed gear; teeth on the plurality of site gears are respectively meshed with teeth on the rotating gear and teeth on the fixed gear, so that the site gears are driven by the rotating gear to rotate along the circumferential direction of the rotating gear and simultaneously rotate along the central axis of the site gears; a containing groove hole is formed in the site gear, and a sample tube containing a substance to be eluted is placed in the containing groove hole; the liquid injection mechanism comprises a liquid injection pipe used for outputting eluent, and the eluent can be added into the sample pipe located below the liquid injection pipe through the liquid injection pipe. The invention solves the technical problems of complex structure, tedious elution process, slow elution speed and high cost of an elution device in nucleic acid extraction.

Description

Technical Field

[0001] The invention relates to the technical field of nucleic acid extraction, and in particular to an elution device for automatic nucleic acid extraction. Background Art

[0002] Nucleic acids are divided into deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is mainly concentrated in the cell nucleus (mitochondria and chloroplasts), while RNA is mainly distributed in the cytoplasm. Nucleic acids, as the material basis of gene expression, are the main object of molecular biology research. Whether it is the structure or function of nucleic acids, it is first necessary to release the nucleic acids in the sample to be tested from the cells, and further separate the released nucleic acids from other impurities in the cells to obtain purer nucleic acids. This process is called nucleic acid extraction and purification. Nucleic acid extraction provides the basis for a large number of extensive research and applications.

[0003] There are three main types of nucleic acid extraction methods: solution extraction, column extraction, and magnetic bead purification. The most commonly used method is magnetic bead purification. Figure 1 As shown, a lysis solution is added to the sample tube containing the sample to release the nucleic acid in the cell nucleus; then magnetic beads are added to the sample tube so that the nucleic acid is adsorbed on the magnetic beads, and the magnetic beads adsorbed with the nucleic acid are enriched on one side of the sample tube through a magnet; then, the waste liquid in the sample tube is removed and a cleaning solution is added to wash the nucleic acid; then, the waste liquid after washing the nucleic acid is removed and an elution solution is added to separate the nucleic acid from the magnetic beads; finally, the magnetic beads are removed to obtain purified nucleic acid. The whole process can be simplified as: lysis, washing, and elution.

[0004] At present, many types of nucleic acid extractors have been produced based on the principle of magnetic bead purification, which are used to extract and purify nucleic acids from samples to be tested. Among them, the types of nucleic acid extractors include multi-sample simultaneous extraction type, single sample separate extraction type and multi-sample on-demand extraction type. Nucleic acid extraction instruments that can extract nucleic acids from multiple samples simultaneously can complete the extraction of nucleic acids from multiple samples at one time. However, they need to collect a sufficient number of samples before they can start running, and cannot be used for on-demand testing. Although nucleic acid extraction instruments that can extract single samples do not need to collect a sufficient number of samples, their processing capacity is limited when faced with a situation where the sample volume is too large, and they cannot quickly complete the nucleic acid extraction. Nucleic acid extraction instruments that can extract nucleic acids from multiple samples on a "come and go" basis can, on the one hand, realize the on-demand extraction of samples without waiting for the collection of a sufficient number of samples, and on the other hand, can also be used in situations where the sample volume is too large, and have good development prospects. However, the existing technology of on-demand nucleic acid extraction instruments is not yet mature, and the development of nucleic acid extraction instruments is still in its early stages. There are many problems. For example, when eluting nucleic acids from the samples to be tested that have completed lysis and cleaning treatments, the elution device is complicated, which leads to a cumbersome elution process and a slow elution speed. In addition, the elution device is bulky and the cost is too high.

[0005] In the related technology, there is no effective solution to the problems of complex structure of elution device, cumbersome elution process, slow elution speed and high cost in nucleic acid extraction.

[0006] Therefore, the present invention proposes an elution device for automated nucleic acid extraction to overcome the defects of the prior art. Summary of the invention

[0007] The object of the present invention is to provide an elution device for automated nucleic acid extraction, which can realize the on-demand collection of samples to be tested and accelerate the elution rate of nucleic acid extraction. At the same time, when applied to scenarios with huge sample volumes, the samples to be tested will not be unable to be processed in time. In addition, the design of the device is more streamlined, the volume is reduced, the cost is lower, and because of its streamlined structure, the operation process is further simplified, the elution rate is improved, and it has a better use effect.

[0008] The purpose of the present invention can be achieved by adopting the following scheme:

[0009] The present invention provides an elution device for automated nucleic acid extraction, the elution device for automated nucleic acid extraction comprising:

[0010] An elution turntable is provided with a rotatable rotating gear, an annular fixed gear is provided around the outer periphery of the rotating gear along its circumference, a plurality of position gears are provided between the rotating gear and the fixed gear, the plurality of position gears are spaced apart and the teeth located on the annular outer edges of the position gears are respectively meshed with the teeth located on the annular outer edges of the rotating gear and the teeth located on the annular inner edges of the fixed gear, so that the plurality of position gears are driven by the rotating gear to rotate along the circumference of the rotating gear while the plurality of position gears rotate along their own central axes;

[0011] The site gear is provided with a receiving slot hole, and the receiving slot hole is used to place a sample tube containing the substance to be eluted;

[0012] The liquid injection mechanism includes an injection tube for outputting the eluent, the injection tube is located above the elution turntable, and when the rotating gear stops rotating, the bottom injection port of the injection tube is vertically opposite to the top opening of the sample tube located on the position gear, so as to add the eluent into the sample tube located below it through the injection tube.

[0013] In a preferred embodiment of the present invention, the elution turntable comprises an elution bottom plate with an opening at the top and a sealing at the bottom, wherein the elution bottom plate has a receiving space intersecting with the opening at the top of the elution bottom plate, and the rotating gear, the fixed gear and the plurality of position gears are all located in the receiving space;

[0014] The fixed gear is connected to the inner wall of the elution turntable to fix the fixed gear;

[0015] A first driving motor is disposed below the elution turntable, and a driving shaft of the first driving motor vertically passes through the bottom wall of the elution turntable from bottom to top and is connected to the central axis of the rotating gear.

[0016] In a preferred embodiment of the present invention, the elution turntable further comprises an elution turntable cover, the elution turntable cover is arranged on the top of the elution bottom plate, and the elution turntable cover is provided with at least one liquid injection operation hole, at least one sample loading operation hole and at least one sample output operation hole, and the distances between the liquid injection operation hole, the sample loading operation hole and the sample output operation hole and the central axis of the rotating gear are all equal to the distance between the accommodating slot and the central axis of the rotating gear;

[0017] The bottom injection port of the injection tube is vertically opposite to the injection operation hole, and the injection operation hole is used to add the eluent into the sample tube in the accommodating slot when the accommodating slot is rotated to be vertically opposite to the injection operation hole;

[0018] The sample loading operation hole is used to place the sample tube containing the substance to be eluted into the accommodating slot when the accommodating slot is rotated to be vertically opposite to the sample loading operation hole;

[0019] The sample discharging operation hole is used to take out the sample tube from the accommodating slot when the accommodating slot is rotated to be vertically opposite to the sample discharging operation hole.

[0020] In a preferred embodiment of the present invention, along the rotation direction of the rotating gear, the liquid injection operation hole, the sample discharge operation hole and the sample loading operation hole are sequentially arranged at intervals along the circumference of the elution disk cover.

[0021] In a preferred embodiment of the present invention, the liquid injection operation hole and the sample output operation hole are centrally symmetrically arranged, and the sample loading operation hole is located between the liquid injection operation hole and the sample output operation hole.

[0022] In a preferred embodiment of the present invention,

[0023] The fixed gear is an internal gear;

[0024] The rotating gear is an external gear;

[0025] The position gear is an external gear or an internal and external gear.

[0026] In a preferred embodiment of the present invention, the injection mechanism also includes a liquid storage container, the liquid outlet of the liquid storage container is connected to the injection tube via an infusion pipeline, and a pumping motor is provided on the infusion pipeline, and the pumping motor is used to pump the eluent stored in the liquid storage container to the injection tube.

[0027] In a preferred embodiment of the present invention, the injection mechanism further includes a robotic arm, a fixed end of the robotic arm is connected to the elution turntable, and the injection tube is disposed at a movable end of the robotic arm to adjust the position of the injection tube.

[0028] In a preferred embodiment of the present invention, the robotic arm includes a mounting base, the liquid storage container is arranged on the mounting base, and the mounting base is provided with a vertical pole, a lifting arm that can move vertically is provided on the vertical pole, a cross arm is provided on the top of the lifting arm, and the pumping motor and the injection pipe are both provided on the cross arm.

[0029] In a preferred embodiment of the present invention, a guide rail extending in the vertical direction is provided on the vertical pole, and the lifting arm is slidably connected to the guide rail;

[0030] A second drive motor is arranged on the mounting base, a drive shaft of the second drive motor is vertically upward and connected to the bottom end of a vertically arranged screw rod, the cross arm is connected to the top end of the lifting arm, a connecting block is arranged at the bottom end of the lifting arm, the connecting block has a threaded hole, and the connecting block is screwed to the screw rod through the threaded hole.

[0031] As described above, the characteristics and advantages of the elution device for automated nucleic acid extraction of the present invention are:

[0032] A rotatable rotating gear is arranged on the elution turntable, a fixed gear is arranged on the outer peripheral ring of the rotating gear, and a plurality of position gears are arranged between the rotating gear and the fixed gear. A sample tube containing a substance to be eluted can be placed in a receiving slot on each of the plurality of position gears. The rotating gear can drive the plurality of position gears to rotate along the circumferential direction of the rotating gear, and the plurality of position gears can also rotate at high speed along their own central axis, so that after the injection mechanism adds the eluent to the sample tube below it, the substance to be eluted in the sample tube can be fully contacted with the eluent, so that the nucleic acid and the adsorbent in the substance to be eluted are quickly and fully separated to obtain purified nucleic acid, which can realize the on-demand extraction of the substance to be eluted and accelerate the elution rate of nucleic acid extraction. When applied to a scene with a huge amount of samples, the sample to be tested will not be unable to be processed in time. In addition, the structure of the present invention is more streamlined, the volume is reduced, the cost is lower, and the simplicity of the structure further simplifies the operation process of nucleic acid extraction, improves the elution rate, and has a better use effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The following drawings are only intended to illustrate and explain the present invention, and are not intended to limit the scope of the present invention.

[0034] in:

[0035] Figure 1 : It is the operation flow chart of the magnetic bead purification method in the prior art.

[0036] Figure 2 : One of the stereoscopic views of the elution device for automated nucleic acid extraction of the present invention.

[0037] Figure 3 : A top view of the internal structure of the elution turntable in the elution device for automated nucleic acid extraction of the present invention.

[0038] Figure 4 : is a schematic cross-sectional view of the elution turntable in the elution device for automated nucleic acid extraction of the present invention.

[0039] Figure 5 : This is the second stereoscopic diagram of the elution device for automated nucleic acid extraction of the present invention.

[0040] Figure 6 : It is a structural schematic diagram of the liquid injection mechanism in the elution device for automated nucleic acid extraction of the present invention.

[0041] The accompanying drawings in the present invention are:

[0042] 1. Liquid injection mechanism; 101. Mounting base;

[0043] 102. A second driving motor; 103. A liquid storage container;

[0044] 104, pole; 1041, guide rail;

[0045] 105. lifting arm; 1051. connecting block;

[0046] 106. Screw rod; 107. Cross arm;

[0047] 108. Pumping motor; 109. Liquid injection pipe;

[0048] 2. Elution turntable; 201. Elution bottom tray;

[0049] 202, elution plate cover; 2021, liquid injection operation hole;

[0050] 2022, sample loading operation hole; 2023, sample discharging operation hole;

[0051] 203, a first driving motor; 2031, a driving shaft;

[0052] 204, driving shaft connecting piece; 205, rotating gear;

[0053] 206, fixed gear; 207, position gear;

[0054] 3. Sample tube. DETAILED DESCRIPTION

[0055] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described with reference to the accompanying drawings.

[0056] The present invention aims to provide an elution device for automated nucleic acid extraction, which is easy to operate, has a fast elution rate, is integrated, streamlined, and has a low cost. The device is used to extract nucleic acid from the nucleic acid after it has been completed. Figure 1 The sample to be tested after lysis and washing treatment is eluted to separate the nucleic acid from the adsorbent (magnetic beads) to obtain purified nucleic acid.

[0057] The elution device for automated nucleic acid extraction of the present invention can, on the one hand, realize the on-demand collection of samples to be tested, thereby accelerating the elution rate of nucleic acid extraction, and at the same time, when a huge amount of samples is used, there will be no situation where the samples to be tested cannot be processed in time; on the other hand, the structural design of the elution device is simpler, the volume is reduced, and the cost is reduced. At the same time, due to its streamlined structure, the operation process is further simplified, and the elution rate is effectively improved.

[0058] like Figures 2 to 6 As shown, the present invention provides an elution device for automatic nucleic acid extraction, the elution device for automatic nucleic acid extraction comprises: an elution turntable 2, a rotatable rotating gear 205 is arranged on the elution turntable 2, a circular ring-shaped fixed gear 206 is arranged around the outer periphery of the rotating gear 205 along its circumference, a plurality of position gears 207 are arranged between the rotating gear 205 and the fixed gear 206, the plurality of position gears 207 are arranged at intervals, and the teeth located on the annular outer edges of the position gears 207 are respectively meshed with the teeth located on the annular outer edges of the rotating gear 205 and the teeth located on the annular inner edges of the fixed gear 206, so as to drive the plurality of position gears 207 to rotate. While the spot gear 207 rotates along the circumference of the rotating gear 205, multiple site gears 207 rotate along their own central axes; a receiving slot hole (not shown) is provided on the site gear 207, and the receiving slot hole is used to place the sample tube 3 containing the substance to be eluted; the injection mechanism 1, the injection mechanism 1 includes an injection tube 109 for outputting the eluent, the injection tube 109 is located above the elution turntable 2, and when the rotating gear 205 stops rotating, the bottom injection port of the injection tube 109 is vertically opposite to the top opening of a sample tube 3 located on the site gear 207, so as to add the eluent to the sample tube 3 located below it through the injection tube 109.

[0059] In the present invention, a rotatable rotating gear 205 is provided on the elution turntable 2, and a fixed gear 206 is provided on the outer peripheral ring of the rotating gear 205. A plurality of site gears 207 are provided between the rotating gear 205 and the fixed gear 206. A sample tube 3 containing a substance to be eluted can be placed in a receiving slot on each of the plurality of site gears 207. Since the teeth on the plurality of site gears 207 are respectively meshed with the teeth on the rotating gear 205 and the teeth on the fixed gear 206, the rotation of the rotating gear 205 can drive the plurality of site gears 207 to rotate along the circumferential direction of the rotating gear 205. At the same time, the plurality of site gears 207 will rotate at a high speed along their own central axis. After the injection mechanism 1 adds the eluent to the sample tube 3 rotating thereunder, the substance to be eluted in the sample tube 3 can be fully contacted with the eluent, so that the nucleic acid in the substance to be eluted and the adsorbent (magnetic beads) are quickly and fully separated to obtain purified nucleic acid. The elution device for automated nucleic acid extraction of the present invention can realize the on-demand extraction of materials to be eluted, thereby accelerating the elution rate of nucleic acid extraction. When applied to scenarios with a huge amount of samples, the samples to be tested will not be unable to be processed in time. In addition, the structure of the present invention is more streamlined, the volume is reduced, the cost is lower, and the simplicity of the structure further simplifies the operation process of nucleic acid extraction, improves the elution rate, and has a better use effect.

[0060] In the present invention, the substance to be eluted contained in the sample tube 3 is nucleic acid adsorbed on the magnetic beads, and the elution liquid used can be but is not limited to TE (Tris-EDTA) buffer.

[0061] In an optional embodiment of the present invention, Figures 2 to 5 As shown, the elution turntable 2 includes a circular elution chassis 201 with an opening at the top and a sealed bottom. The elution chassis 201 has an accommodating space that is connected to the top opening of the elution chassis 201, and a rotating gear 205, a fixed gear 206 and a plurality of position gears 207 are all located in the accommodating space; the fixed gear 206 is connected to the inner wall of the elution turntable 2 or other positions of the elution turntable 2 to fix the fixed gear 206, so that the fixed gear 206 is always in a stationary state relative to the elution turntable 2; a first driving motor 203 is arranged below the elution turntable 2, and a driving shaft 2031 of the first driving motor 203 vertically passes through the bottom wall of the elution turntable 2 from bottom to top and is connected to the central axis of the rotating gear 205, and the driving shaft 2031 of the first driving motor 203 can drive the rotating gear 205 to rotate around its central axis (that is, the rotating gear 205 can be in a rotating state relative to the elution turntable 2).

[0062] Further, the driving shaft 2031 of the first driving motor 203 may be connected to the bottom wall of the elution turntable 2 via a driving shaft connector 204. The driving shaft connector 204 may be, but is not limited to, a bearing rotatably disposed on the bottom wall of the elution turntable 2, and the driving shaft 2031 of the first driving motor 203 rotatably passes through the bottom wall of the elution turntable 2 via the bearing.

[0063] In this embodiment, if Figure 3 , Figure 4 As shown, the fixed gear 206 is an internal gear; the rotating gear 205 is an external gear; and the position gear 207 is an external gear or an internal and external gear. In the specific working process, the driving shaft 2031 of the first driving motor 203 can drive the rotating gear 205 to rotate clockwise, while the fixed gear 206 does not rotate, so that the multiple position gears 207 rotate clockwise around the circumference of the rotating gear 205, and the multiple position gears 207 also rotate counterclockwise, so as to achieve the purpose that the material to be eluted can be fully contacted with the eluent, so that the nucleic acid in the material to be eluted and the adsorbent (magnetic beads) are quickly and fully separated. Among them, the type of the rotating gear 205 must be an external gear and must rotate, the type of the fixed gear 206 must be an internal gear and must not move, and the type of the position gear 207 can be an external gear or an internal and external gear, and the position gear 207 is located between the rotating gear 205 and the fixed gear 206, and can be driven to rotate.

[0064] In an optional embodiment of the present invention, Figure 2 , Figure 5 As shown, the elution turntable 2 also includes a circular elution disk cover 202, which is detachably covered on the top of the elution base 201. The elution disk cover 202 is provided with at least one liquid injection operation hole 2021, at least one sample loading operation hole 2022 and at least one sample discharge operation hole 2023. The distances between the liquid injection operation hole 2021, the sample loading operation hole 2022 and the sample discharge operation hole 2023 and the central axis of the rotating gear 205 are all equal to the distance between the accommodating slot hole and the central axis of the rotating gear 205, so that under the drive of the rotating gear 205, any accommodating slot hole can be rotated to a position vertically opposite to the liquid injection operation hole 2021, the sample loading operation hole 2022 and the sample discharge operation hole 2023, respectively, so as to facilitate the injection, sample loading, sample discharge and other operations on the sample tube 3 placed in the accommodating slot hole.

[0065] Specifically, Figure 2 , Figure 5As shown, the bottom injection port of the injection tube 109 is vertically opposite to the injection operation hole 2021. The injection operation hole 2021 can be used to add eluent to the sample tube 3 in the accommodating slot when a accommodating slot is rotated to be vertically opposite to the injection operation hole 2021; the loading operation hole 2022 is used to place the sample tube 3 containing the material to be eluted in the accommodating slot when a accommodating slot is rotated to be vertically opposite to the loading operation hole 2022; the sample outlet operation hole 2023 is used to take out the sample tube 3 from the accommodating slot when a accommodating slot is rotated to be vertically opposite to the sample outlet operation hole 2023.

[0066] In a specific embodiment of the present invention, Figure 2 , Figure 5 As shown, along the rotation direction of the rotating gear 205, the liquid injection operation hole 2021, the sample output operation hole 2023 and the sample loading operation hole 2022 are sequentially arranged at intervals along the circumference of the elution disk cover 202. Among them, the liquid injection operation hole 2021 and the sample output operation hole 2023 are arranged in a central symmetric manner (that is, in the circumference of the elution disk cover 202, the angle between the liquid injection operation hole 2021 and the sample output operation hole 2023 is 180°), and the sample loading operation hole 2022 is located between the liquid injection operation hole 2021 and the sample output operation hole 2023.

[0067] In a specific embodiment of the present invention, Figure 2 , Figure 5 , Figure 6 As shown, the injection mechanism 1 also includes a liquid storage container 103, the liquid outlet of the liquid storage container 103 is connected to the injection tube 109 through an infusion pipeline, and a pumping motor 108 is provided on the infusion pipeline. The pumping motor 108 is used to pump the eluent stored in the liquid storage container 103 to the injection tube 109, so as to add the eluent to the corresponding sample tube 3. By controlling the working state of the pumping motor 108, the amount of eluent added to the sample tube 3 can be controlled to improve the accuracy of adding the eluent, ensure that the nucleic acid in the eluted substance can be quickly and fully separated from the adsorbent (magnetic beads), and obtain purified nucleic acid.

[0068] In an optional embodiment of the present invention, Figure 2 , Figure 5 , Figure 6 As shown, the injection mechanism 1 also includes a robotic arm having a fixed end and a movable end, the fixed end of the robotic arm is connected to the elution turntable 2, and the injection tube 109 is arranged at the movable end of the robotic arm. The robotic arm can drive the injection tube 109 to adjust its position to ensure that the bottom injection port of the injection tube 109 can be located at a position vertically opposite to the injection operation hole 2021, so as to accurately add the eluent.

[0069] Specifically, Figure 2 , Figure 5 , Figure 6As shown, the robotic arm includes a mounting base 101, which is fixedly arranged on the top surface of the elution plate cover 202, and a liquid storage container 103 is arranged on the mounting base 101, and a vertically extending vertical pole 104 is arranged on the mounting base 101, and a vertically movable lifting arm 105 is arranged on the vertical pole 104, and a horizontal arm 107 extending in the horizontal direction is arranged on the top of the lifting arm 105, and a pumping motor 108 and an injection pipe 109 are both arranged on the horizontal arm 107. By adjusting the lifting height of the lifting arm 105 on the vertical pole 104, the height of the horizontal arm 107 can be adjusted to achieve the purpose of adjusting the height of the injection pipe 109.

[0070] Further, such as Figure 2 , Figure 5 , Figure 6 As shown, a guide rail 1041 extending vertically is provided on the vertical pole 104, and the lifting arm 105 can be slidably connected to the guide rail 1041; a second drive motor 102 is provided on the mounting base 101, and the driving shaft of the second drive motor 102 is vertically upward and connected to the bottom end of the vertically arranged lead screw 106, the cross arm 107 is connected to the top end of the lifting arm 105, and a connecting block 1051 is provided at the bottom end of the lifting arm 105 (the lifting arm 105 and the connecting block 1051 can be integrally formed in an "L" shape), and a threaded hole is provided on the connecting block 1051, and the connecting block 1051 is screwed to the lead screw 106 through the threaded hole. During actual operation, the second drive motor 102 drives the screw rod 106 to rotate. Since the lifting arm 105 is screwed to the screw rod 106 through the connecting block 1051, and the lifting arm 105 is limited in the circumferential direction, when the screw rod 106 rotates, the lifting arm 105 can only rise or fall along the screw rod 106 according to the rotation direction of the screw rod 106, so as to achieve the purpose of adjusting the height of the injection tube 109.

[0071] The working process of the elution device for automated nucleic acid extraction of the present invention is as follows:

[0072] During the entire working process, the first drive motor 203 is always in working state and does not shut down. Figures 3 to 5As shown, the sample tube 3 containing the material to be eluted (the material to be eluted has been subjected to lysis treatment and cleaning treatment) is placed in the receiving slot on the vertically corresponding site gear 207 at the sample loading operation hole 2022; with the rotation of the rotating gear 205, multiple site gears 207 rotate along the circumferential rotation of the rotating gear 205 while rotating on their own. When the next site gear 207 rotates to a position vertically opposite to the sample loading operation hole 2022, the next sample tube 3 containing the material to be eluted is placed in the receiving slot on the corresponding site gear 207, and the sample loading operation is similar; with the continuous rotation of the rotating gear 205, when the sample tube 3 reaches a position vertically opposite to the injection operation hole 2021 from the position vertically opposite to the sample loading operation hole 2022 as the site gear 207 rotates, the injection mechanism 1 is controlled by The injection tube 109 injects the eluent into the sample tube 3; thereafter, as the rotating gear 205 continues to rotate, when the sample tube 3 leaves the position vertically opposite to the injection operation hole 2021 as the site gear 207 rotates and before reaching the position vertically opposite to the sample output operation hole 2023, because the sample tube 3 always rotates along with the site gear 207, the substance to be eluted in the sample tube 3 can fully contact with the eluent, and the nucleic acid adsorbed on the magnetic beads can be eluted, thereby improving the separation rate of the nucleic acid and the magnetic beads; as the rotating gear 205 continues to rotate, when the sample tube 3 reaches the position vertically opposite to the sample output operation hole 2023 as the site gear 207 rotates, the sample tube 3 opposite to the sample output operation hole 2023 can be taken out manually by controlling the existing manipulator or the operator to complete the nucleic acid elution operation.

[0073] The characteristics and advantages of the elution device for automated nucleic acid extraction of the present invention are:

[0074] 1. The elution device for automated nucleic acid extraction can not only realize the on-demand extraction of the material to be eluted, but also accelerate the elution rate of nucleic acid extraction. When applied to scenarios with huge sample volumes, the sample to be tested will not be unable to be processed in time.

[0075] Second, the elution device for automated nucleic acid extraction has a more streamlined structure, reduced volume, and lower cost. In addition, its streamlined structure further simplifies the operation process of nucleic acid extraction, improves the elution rate, and has a better use effect.

[0076] The above description is only an illustrative embodiment of the present invention and is not intended to limit the scope of the present invention. Any equivalent changes and modifications made by any person skilled in the art without departing from the concept and principle of the present invention shall fall within the scope of protection of the present invention.

Claims

1. An elution device for automated nucleic acid extraction, characterized in that: The elution device for automated nucleic acid extraction comprises: An elution turntable is provided with a rotatable rotating gear, an annular fixed gear is provided around the outer periphery of the rotating gear along its circumference, a plurality of position gears are provided between the rotating gear and the fixed gear, the plurality of position gears are spaced apart and the teeth located on the annular outer edges of the position gears are respectively meshed with the teeth located on the annular outer edges of the rotating gear and the teeth located on the annular inner edges of the fixed gear, so that the plurality of position gears are driven by the rotating gear to rotate along the circumference of the rotating gear while the plurality of position gears rotate along their own central axes; The site gear is provided with a receiving slot hole, and the receiving slot hole is used to place a sample tube containing the substance to be eluted; The liquid injection mechanism includes an injection tube for outputting the eluent, the injection tube is located above the elution turntable, and when the rotating gear stops rotating, the bottom injection port of the injection tube is vertically opposite to the top opening of the sample tube located on the position gear, so as to add the eluent into the sample tube located below it through the injection tube.

2. The elution device for automated nucleic acid extraction according to claim 1, characterized in that: The elution turntable comprises an elution bottom plate with an opening at the top and a sealing at the bottom, wherein the elution bottom plate has a receiving space intersecting with the opening at the top of the elution bottom plate, and the rotating gear, the fixed gear and the plurality of position gears are all located in the receiving space; The fixed gear is connected to the inner wall of the elution turntable to fix the fixed gear; A first driving motor is disposed below the elution turntable, and a driving shaft of the first driving motor vertically passes through the bottom wall of the elution turntable from bottom to top and is connected to the central axis of the rotating gear.

3. The elution device for automated nucleic acid extraction according to claim 2, characterized in that: The elution turntable further comprises an elution turntable cover, the elution turntable cover is arranged on the top of the elution bottom plate, the elution turntable cover is provided with at least one liquid injection operation hole, at least one sample loading operation hole and at least one sample output operation hole, and the distances between the liquid injection operation hole, the sample loading operation hole and the sample output operation hole and the central axis of the rotating gear are all equal to the distance between the accommodating slot and the central axis of the rotating gear; The bottom injection port of the injection tube is vertically opposite to the injection operation hole, and the injection operation hole is used to add the eluent into the sample tube in the accommodating slot when the accommodating slot is rotated to be vertically opposite to the injection operation hole; The sample loading operation hole is used to place the sample tube containing the substance to be eluted into the accommodating slot when the accommodating slot is rotated to be vertically opposite to the sample loading operation hole; The sample discharging operation hole is used to take out the sample tube from the accommodating slot when the accommodating slot is rotated to be vertically opposite to the sample discharging operation hole.

4. The elution device for automated nucleic acid extraction according to claim 3, characterized in that: Along the rotation direction of the rotating gear, the liquid injection operation hole, the sample discharge operation hole and the sample loading operation hole are sequentially arranged at intervals along the circumference of the elution disk cover.

5. The elution device for automated nucleic acid extraction according to claim 4, characterized in that: The liquid injection operation hole and the sample output operation hole are centrally symmetrically arranged, and the sample loading operation hole is located between the liquid injection operation hole and the sample output operation hole.

6. The elution device for automated nucleic acid extraction according to any one of claims 1 to 5, characterized in that: The fixed gear is an internal gear; The rotating gear is an external gear; The position gear is an external gear or an internal and external gear.

7. The elution device for automated nucleic acid extraction according to claim 1, characterized in that: The injection mechanism also includes a liquid storage container, the liquid outlet of the liquid storage container is connected to the injection tube via an infusion pipeline, and a pumping motor is provided on the infusion pipeline. The pumping motor is used to pump the eluent stored in the liquid storage container to the injection tube.

8. The elution device for automated nucleic acid extraction according to claim 7, characterized in that: The injection mechanism also includes a mechanical arm, a fixed end of the mechanical arm is connected to the elution turntable, and the injection tube is arranged at the movable end of the mechanical arm to adjust the position of the injection tube.

9. The elution device for automated nucleic acid extraction according to claim 8, characterized in that: The robotic arm includes a mounting base, the liquid storage container is arranged on the mounting base, and the mounting base is provided with a vertical pole, a lifting arm that can move vertically is provided on the vertical pole, a cross arm is provided on the top of the lifting arm, and the pumping motor and the injection pipe are both provided on the cross arm.

10. The elution device for automated nucleic acid extraction according to claim 9, characterized in that: The vertical rod is provided with a guide rail extending in the vertical direction, and the lifting arm is slidably connected to the guide rail; A second drive motor is arranged on the mounting base, a drive shaft of the second drive motor is vertically upward and connected to the bottom end of a vertically arranged screw rod, the cross arm is connected to the top end of the lifting arm, a connecting block is arranged at the bottom end of the lifting arm, the connecting block has a threaded hole, and the connecting block is screwed to the screw rod through the threaded hole.

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