Preparation method and application of biological composite carbon source

Abstract

The invention belongs to the field of sewage treatment, and specifically relates to a preparation method and application of a biological composite carbon source, wherein the preparation method comprises the following steps: mixing waste beer yeast liquid and biodiesel byproducts, adding enzyme preparations, mixing and reacting, and separating supernatant to obtain the biological composite carbon source. The biological composite carbon source of the invention has low production cost, rich nutrients, strong adaptability, long-term effectiveness, high denitrification rate and denitrification effect.

Description

Technical Field

[0001] The invention belongs to the field of sewage treatment, and specifically relates to a preparation method and application of a biological composite carbon source. Background Art

[0002] Composite carbon sources composed of chemical products can also achieve good results due to their strong customization and good stability. They are now widely used, but some chemical composite carbon sources may produce harmful substances during production and use, posing environmental risks. At the same time, if improperly handled, discarded chemical composite carbon sources may also pollute the environment. Chemical composite carbon sources can quickly provide carbon sources for microorganisms, but their components may not fully match the metabolic pathways of certain specific microorganisms, resulting in restrictions on the growth and reproduction of microorganisms. Biological composite carbon sources are mainly derived from natural organic matter and have good environmental protection. Its use will not cause secondary pollution to the environment and meets the requirements of sustainable development. Biological composite carbon sources usually contain a variety of organic substances and trace elements, which can provide comprehensive nutritional support for microorganisms in sewage treatment systems and promote the growth and reproduction of microorganisms. The composition of biological composite carbon sources is closer to organic matter in nature, so they are more easily adapted and utilized by microorganisms in sewage treatment systems. The preparation of existing biological composite carbon sources, in addition to basic raw materials and enzyme preparations or bacterial agents, also needs to add some other nutrients, such as glucose, peptone, biotin, amino acids, etc., to achieve the purpose of activating activated sludge. CN117923659A prepares biologically active composite carbon sources by compounding glycerol, biological enzyme molecular chaperones, biotin, growth factors and amino acids. Although there are many types of carbon sources at present, the commonly used carbon sources still have deficiencies in terms of cost, denitrification rate, unit consumption, mud production, safety, etc., and it is urgent to develop efficient, economical, safe and convenient organic carbon source products suitable for use in sewage treatment plants in my country. Summary of the invention

[0003] In order to solve the problems in the prior art, such as high carbon source cost, insufficient denitrification rate, unit consumption, sludge production, safety, etc., the present invention mainly provides a preparation method and application of a biological composite carbon source with low production cost, rich nutrients, strong adaptability, long-term effectiveness, high denitrification rate and denitrification effect. The technical scheme is as follows:

[0004] A preparation method and application of a biocomposite carbon source comprises the following steps: mixing waste beer yeast liquid and biodiesel byproducts, adding enzyme preparations, mixing and reacting, and separating supernatant to obtain the biocomposite carbon source.

[0005] Furthermore, the mass ratio of the waste beer yeast liquid to the biodiesel by-product is 10:1-10; and the dosage of the enzyme preparation is 0.5-5 wt %.

[0006] Furthermore, the enzyme preparation is one or more of yeast wall-breaking enzyme, cellulase, xylanase, acid protease, β-glucanase, alkaline protease, lipase, neutral protease and papain.

[0007] Furthermore, the temperature of the mixed reaction is 10-50°C; and the time of the mixed reaction is 20-80 h.

[0008] Furthermore, before adding the enzyme preparation, the pH of the system is adjusted to 6-9.

[0009] Furthermore, before adding the enzyme preparation, a carrier treated with waste beer yeast and biodiesel byproducts is added; the preparation of the carrier comprises the following steps:

[0010] a. Mix metakaolin with sodium hydroxide solution to form granules, then immerse them in biodiesel byproducts for 1-3 hours, continue to heat to 60-80 °C, and stand for 20-30 hours; take out the granules and calcine them at 400-550 °C for 2-4 hours to obtain precursor granules;

[0011] b. Place the precursor particles in the biodiesel byproduct, first heat treat, then react at 220-250℃ for 3-5 h in a nitrogen atmosphere; then cool to 20-40℃, add waste beer yeast solution to the system to double the volume of the system, ultrasonicate for 0.1-1 h, and then take out the particles to obtain the carrier.

[0012] Furthermore, the mass ratio of the carrier to the biodiesel byproduct is 1:1-100; and the mass ratio of the sodium hydroxide to the metakaolin in step a is 1:3-4.

[0013] Furthermore, the heat treatment in step b is ultrasonic mixing at 50-70° C. for 10-30 min.

[0014] A biological composite carbon source prepared by the above preparation method, COD Cr The value is 100000~1000000 mg / L.

[0015] An application of the above-mentioned biological composite carbon source in denitrification treatment of sewage, wherein the sewage includes one or more of domestic sewage, industrial sewage or garbage leachate.

[0016] By adopting the above scheme, the method of the present invention has the following advantages:

[0017] The raw materials of the biocomposite carbon source of the present invention mainly come from brewer's yeast liquid and biodiesel byproducts. The efficient biocomposite carbon source is obtained through the action of enzyme preparations. The biocomposite carbon source has low production cost, rich nutrients, strong adaptability, long-term effectiveness, high denitrification rate and denitrification effect.

[0018] The unit consumption ΔCOD / ΔTN value of the biocomposite carbon source of the present invention is lower than that of carbon sources such as sodium acetate, acetic acid, and glucose, but under the same conditions, the dosage of the biocomposite carbon source of the present invention is about 20% lower than that of the sodium acetate carbon source for removing unit TN or nitrate. Therefore, the use of the carbon source of the present invention can save operating costs and excess sludge generation.

[0019] COD of the biological composite carbon source of the present invention Cr It is mainly composed of waste by-products from the fermentation industry's production process, which greatly reduces the production cost of the carbon source. It has cost advantages over common carbon sources on the market such as sodium acetate, acetic acid, ethylene glycol, propylene glycol and glucose.

[0020] The present invention uses metakaolin as the matrix of carrier particles and utilizes biodiesel byproducts as pore-forming agents to increase the porosity of the carrier particles, facilitate the attachment of bacteria in the denitrification process, promote bacterial reproduction and fixation, improve sewage treatment efficiency, reduce the bacterial content in effluent water, and prevent secondary increases in water indicators.

[0021] The invention utilizes the amphiphilicity of soap groups in biodiesel byproducts to improve the affinity of the metakaolin matrix with the biodiesel byproducts, beer yeast liquid and wastewater to be treated, thereby ensuring the sewage treatment efficiency.

[0022] The present invention polymerizes glycerol in the biodiesel byproduct on carrier particles to improve the strength of the carrier particles, and the polymerized glycerol can be blocked in pores with smaller pore sizes, while glycerol or polymerized glycerol in larger pores is replaced by brewer's yeast liquid, so that a richer nutrient environment is formed in the carrier particles, which is convenient for the reproduction of bacteria.

[0023] The raw materials used in the present invention are safe and non-toxic. After meeting the carbon source demand in the denitrification process of the anoxic pool, the remaining carbon source can also be quickly degraded by heterotrophic bacteria in the aerobic pool, and the effluent COD Cr It complies with national emission standards, the product is safe and non-toxic, and will not cause environmental ecological harm or human poisoning. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a comparison chart of unit consumption of sodium acetate, acetic acid, Example 1, Examples 4-6 and Examples 9-10;

[0025] Figure 2 It is a comparison chart of the unit consumption of sodium acetate, Example 2, Example 7-8 and Example 11;

[0026] Figure 3 It is a comparison chart of the unit consumption of glucose and Example 3. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Example 1: By mass, 10 parts of waste yeast liquid from brewer, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into a reactor and mixed evenly, and reacted at 30°C (stirring speed 100 r / min). After reacting for 48 h, centrifugal solid-liquid separation was performed (speed 5000 r / min, separation 10 min) to obtain a separated liquid, which was the biocomposite carbon source.

[0029] Example 2: By mass, 10 parts of waste yeast liquid from brewer, 2 parts of biodiesel by-product, 0.1 parts of yeast wall-breaking enzyme and 0.1 parts of cellulase were added into a reactor and mixed evenly, and reacted at 30°C (stirring speed 100 r / min). After reacting for 48 hours, centrifugal solid-liquid separation was performed (speed 5000 r / min, separation 10 min) to obtain a separated liquid, which is the biocomposite carbon source.

[0030] Example 3: By mass, 10 parts of waste yeast liquid from brewer, 4 parts of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into a reactor and mixed evenly, and reacted at 30°C (stirring speed 100 r / min). After the reaction for 48 h, centrifugal solid-liquid separation was performed (speed 5000 r / min, separation 10 min) to obtain a separated liquid, which is the biocomposite carbon source.

[0031] Example 4: (1) 1 g of sodium hydroxide was used to prepare a saturated solution, 3 g of metakaolin was placed in the saturated sodium hydroxide solution and mixed to form granules, and then the obtained granules were immersed in biodiesel byproducts for 2 h, the system was further heated to 70°C and allowed to stand for 24 h; the granules were taken out and calcined at 500°C for 3 h to obtain precursor granules;

[0032] (2) The precursor particles are placed in the biodiesel byproduct and first ultrasonically mixed at 70°C for 30 minutes; then the precursor particles are taken out and reacted at 230°C for 3 hours under a nitrogen atmosphere; then the temperature is lowered to 40°C, and waste beer yeast liquid is added to the system to double the volume of the system. After ultrasonication for 0.5 hours, the particles are taken out to obtain the carrier;

[0033] (3) By weight, 1 part of carrier, 10 parts of waste yeast liquid from brewer, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into a reactor and mixed evenly. The mixture was reacted at 30°C (stirring speed: 100 r / min). After reacting for 48 h, centrifugal solid-liquid separation was performed (speed: 5000 r / min, separation time: 10 min) to obtain a separated liquid, which was the biocomposite carbon source.

[0034] Example 5: (1) 1 g of sodium hydroxide was used to prepare a saturated solution, 3 g of metakaolin was placed in the saturated sodium hydroxide solution and mixed to form granules, and then the obtained granules were immersed in biodiesel byproducts for 2 h, the system was further heated to 70 °C and allowed to stand for 24 h; the granules were taken out and calcined at 500 °C for 3 h to obtain precursor granules;

[0035] (2) The precursor particles were placed in the biodiesel byproducts and first ultrasonically mixed at 50 °C for 30 min; then the precursor particles were taken out and reacted at 230 °C for 3 h under a nitrogen atmosphere; then the temperature was lowered to 40 °C, and waste brewer's yeast liquid was added to the system to double the volume of the system. After ultrasonication for 0.5 h, the particles were taken out to obtain the carrier;

[0036] (3) By mass, 1 part of carrier, 10 parts of waste yeast liquid, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into the reactor and mixed evenly. The mixture was reacted at 30 °C (stirring speed 100 r / min). After the reaction time of 48 h, the solid-liquid separation was carried out by centrifugation (speed 5000 r / min, separation time 10 min) to obtain the separated liquid, which was the biocomposite carbon source.

[0037] Example 6: (1) 1 g of sodium hydroxide was used to prepare a saturated solution, 3 g of metakaolin was placed in the saturated sodium hydroxide solution and mixed to form granules, and then the obtained granules were immersed in biodiesel byproducts for 2 h, the system was further heated to 70 °C and allowed to stand for 24 h; the granules were taken out and calcined at 500 °C for 3 h to obtain precursor granules;

[0038] (2) The precursor particles were placed in the biodiesel byproducts and first ultrasonically mixed at 70 °C for 10 min. The precursor particles were then taken out and reacted at 230 °C for 3 h in a nitrogen atmosphere. The temperature was then lowered to 40 °C, and waste brewer's yeast liquid was added to the system to double the volume of the system. After ultrasonication for 0.5 h, the particles were taken out to obtain the carrier.

[0039] (3) By mass, 1 part of carrier, 10 parts of waste yeast liquid, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into the reactor and mixed evenly. The mixture was reacted at 30 °C (stirring speed 100 r / min). After the reaction time of 48 h, the solid-liquid separation was carried out by centrifugation (speed 5000 r / min, separation time 10 min) to obtain the separated liquid, which was the biocomposite carbon source.

[0040] Example 7: (1) 1 g of sodium hydroxide was used to prepare a saturated solution, 3 g of metakaolin was placed in the saturated sodium hydroxide solution and mixed to form granules, and then the obtained granules were immersed in biodiesel byproducts for 2 h, the system was further heated to 70°C and allowed to stand for 24 h; the granules were taken out and calcined at 500°C for 3 h to obtain precursor granules;

[0041] (2) The precursor particles are placed in the biodiesel byproduct and first ultrasonically mixed at 70°C for 30 minutes; then the precursor particles are taken out and reacted at 230°C for 3 hours under a nitrogen atmosphere; then the temperature is lowered to 20°C, and waste brewer's yeast liquid is added to the system to double the volume of the system. After ultrasonication for 0.5 hours, the particles are taken out to obtain the carrier;

[0042] (3) By mass, 1 part of carrier, 10 parts of waste yeast liquid, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into the reactor and mixed evenly. The mixture was reacted at 30 °C (stirring speed 100 r / min). After the reaction time of 48 h, the solid-liquid separation was carried out by centrifugation (speed 5000 r / min, separation time 10 min) to obtain the separated liquid, which was the biocomposite carbon source.

[0043] Example 8: (1) 1 g of sodium hydroxide was used to prepare a saturated solution, 3 g of metakaolin was placed in the saturated sodium hydroxide solution and mixed to form granules, and then the obtained granules were immersed in biodiesel byproducts for 2 h, the system was further heated to 70 °C and allowed to stand for 24 h; the granules were taken out and calcined at 500 °C for 3 h to obtain precursor granules;

[0044] (2) The precursor particles were placed in the biodiesel byproduct and first ultrasonically mixed at 70 °C for 30 min. The precursor particles were then taken out and reacted at 230 °C for 3 h under a nitrogen atmosphere. The temperature was then lowered to 40 °C, and waste brewer's yeast liquid was added to the system to double the volume of the system. After ultrasonication for 0.1 h, the particles were taken out to obtain the carrier.

[0045] (3) By mass, 1 part of carrier, 10 parts of waste yeast liquid from brewer, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into the reactor and mixed evenly. The mixture was reacted at 30 °C (stirring speed 100 r / min). After 48 h of reaction, the mixture was centrifuged for solid-liquid separation (speed 5000 r / min, separation 10 min) to obtain the separated liquid, which was the biocomposite carbon source.

[0046] Example 9: (1) 1 g of sodium hydroxide was used to prepare a saturated solution, 3 g of metakaolin was placed in the saturated sodium hydroxide solution and mixed to form granules, and then the obtained granules were immersed in biodiesel byproducts for 2 h, the system was further heated to 70 °C and allowed to stand for 24 h; the granules were taken out and calcined at 500 °C for 3 h to obtain a carrier;

[0047] (2) By mass, 1 part of carrier, 10 parts of waste yeast liquid from brewer, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into the reactor and mixed evenly. The mixture was reacted at 30 °C (stirring speed 100 r / min). After 48 h of reaction, the mixture was centrifuged for solid-liquid separation (speed 5000 r / min, separation 10 min) to obtain the separated liquid, which was the biocomposite carbon source.

[0048] Example 10: (1) 1 g of sodium hydroxide was used to prepare a saturated solution, 3 g of metakaolin was placed in the saturated sodium hydroxide solution, and the mixture was granulated. The granules were calcined at 500 °C for 3 h to obtain precursor granules.

[0049] (2) The precursor particles were placed in the biodiesel byproduct and first ultrasonically mixed at 70 °C for 30 min. The precursor particles were then taken out and reacted at 230 °C for 3 h under a nitrogen atmosphere. The temperature was then lowered to 40 °C, and waste brewer's yeast liquid was added to the system to double the volume of the system. After ultrasonication for 0.5 h, the particles were taken out to obtain the carrier.

[0050] (3) By mass, 1 part of carrier, 10 parts of waste yeast liquid, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into the reactor and mixed evenly. The mixture was reacted at 30 °C (stirring speed 100 r / min). After the reaction time of 48 h, the solid-liquid separation was carried out by centrifugation (speed 5000 r / min, separation time 10 min) to obtain the separated liquid, which was the biocomposite carbon source.

[0051] Example 11: (1) 1 g of sodium hydroxide was used to prepare a saturated solution, 3 g of metakaolin was placed in the saturated sodium hydroxide solution and mixed to form granules, and then the obtained granules were immersed in biodiesel byproducts for 2 h, the system was further heated to 70 °C and allowed to stand for 24 h; the granules were taken out and calcined at 500 °C for 3 h to obtain precursor granules;

[0052] (2) The precursor particles were placed in the biodiesel byproduct and firstly ultrasonically mixed at 70 °C for 30 min; then the precursor particles were taken out and reacted at 230 °C for 3 h under a nitrogen atmosphere, and the particles were taken out to obtain the carrier;

[0053] (3) By mass, 1 part of carrier, 10 parts of waste yeast liquid, 1 part of biodiesel by-product, 0.1 part of yeast wall-breaking enzyme and 0.1 part of cellulase were added into the reactor and mixed evenly. The mixture was reacted at 30 °C (stirring speed 100 r / min). After the reaction time of 48 h, the solid-liquid separation was carried out by centrifugation (speed 5000 r / min, separation time 10 min) to obtain the separated liquid, which was the biocomposite carbon source.

[0054] Example sample test:

[0055] The mixed liquid at the end of the aerobic pool of the sewage treatment plant was taken and a beaker test was carried out using sodium acetate, acetic acid, Example 1, Examples 4-6 and Examples 9-10 as carbon sources. Cr The concentration of Y was 15 mg / L, TN was 13.32 mg / L, ammonia nitrogen was 4.74 mg / L, nitrate nitrogen was 6.93 mg / L, and sludge concentration SS was 6243 mg / L. The stirring was carried out by a magnetic stirrer. The stirring speed was controlled to maintain the DO of the mixed solution in the beaker below 0.5 mg / L and ensure that the sludge could be evenly mixed. The test was carried out for 4 to 6 hours. N is the denitrification / denitrification rate (mg TN / g SS.h); Y C is the carbon source consumption rate of sludge (mg COD / g SS.h); SS is the sludge concentration (g / L); ΔCOD / ΔTN is the carbon source consumption per unit of denitrification, that is, the carbon source required to remove unit TN. Figure 1 As shown in Table 1:

[0056] Table 1:

[0057]

[0058] from Figure 1 As shown in Table 1, acetic acid and sodium acetate have similar denitrification rates Y N , Carbon source consumption rate Y C and carbon source unit consumption ΔCOD / ΔTN value. At the same time, the denitrification rate Y of each embodiment NAll of them are better than acetic acid and sodium acetate, indicating that the biological composite carbon source of the present invention can be quickly applied to the denitrification of activated sludge denitrifying bacteria, and the denitrification rate can reach or even exceed that of acetic acid and sodium acetate. The unit consumption ΔCOD / ΔTN values ​​of each embodiment are lower than those of acetic acid and sodium acetate, and the unit consumption of embodiments 4 to 6 and embodiments 9 to 10 using carriers is significantly reduced, indicating that the consumption rate of the carbon source can be balanced after using the carrier. Among them, the unit consumption of embodiments 9 and 10 that are not treated with waste beer yeast liquid and biodiesel by-products is close to that of embodiment 1 that is not treated with a carrier, indicating that the untreated carrier has no significant effect on the denitrification process.

[0059] The mixed liquid at the end of the biological reaction aerobic tank was taken and a beaker test was carried out using sodium acetate, Example 2, Example 7-8 and Example 11 as carbon sources. Cr The concentration of nitrogen was 30 mg / L, TN was 17.09 mg / L, nitrate nitrogen was 15.43 mg / L, and sludge concentration SS was 8082 mg / L. The stirring was carried out by a magnetic stirrer. The stirring speed was controlled to maintain the DO of the mixed solution in the beaker below 0.5 mg / L and ensure that the sludge could be evenly mixed. The test lasted for 4 to 6 hours. Figure 2 As shown in Table 2:

[0060] Table 2:

[0061]

[0062] from Figure 2 From Table 2, it can be seen that the denitrification rate of Example 2 is slightly lower than that of sodium acetate carbon source, but the denitrification level is close, and its corresponding Y N 0.61 and 0.75 mg TN / g SS.h respectively, indicating that the composite carbon source of the present invention has practical application value. The denitrification rates of Examples 7, 8, and 11 are higher than that of the sodium acetate carbon source, indicating that the carrier of the present invention can promote the growth and reproduction of denitrifying bacteria. The unit consumption ΔCOD / ΔTN values ​​of Examples 2 and 7, 8, and 11 are lower than those of sodium acetate, indicating that the use of the biological composite carbon source of the present invention can reduce the amount of carbon source added and save the denitrification operating cost. Among them, the unit consumption of Example 11, which is not treated with brewer's yeast waste liquid, is slightly increased compared with Examples 7 and 8, indicating that the step of using brewer's yeast waste liquid to replace part of the biodiesel by-products in the carrier can improve the denitrification capacity of the biological composite carbon source of the present invention.

[0063] The mixed sludge liquid at the end of the biochemical reaction section of a landfill leachate treatment plant in Hangzhou was taken and a beaker test was carried out using glucose and Example 3 as carbon sources. COD of the mixed liquid after nitrogen source supplementation CrThe concentration of nitrate nitrogen was 396 mg / L, TN was 139 mg / L, nitrate nitrogen was 134.35 mg / L, and sludge concentration SS was 5650 mg / L. The stirring was carried out with a magnetic stirrer. The stirring speed was controlled to maintain the DO of the mixed solution in the beaker below 0.5 mg / L and ensure that the sludge could be evenly mixed. The test lasted for 4 to 6 hours. The results are shown in Figure 3 As shown in Table 3:

[0064] Table 3:

[0065]

[0066] From Table 3, the denitrification rate Y N From the above, the denitrification rate of Example 3 is higher than that of glucose, and the unit consumption ΔCOD / ΔTN value is also lower than that of glucose, which indicates that the biocomposite carbon source of the present invention has a high denitrification rate, strong adaptability, and lower cost than that of glucose.

[0067] For those skilled in the art, various other corresponding changes and deformations can be made according to the technical solutions and concepts described above, and all of these changes and deformations should fall within the protection scope of the claims of the present invention.

Claims

1. A method for preparing a biological composite carbon source, characterized in that: The method comprises the following steps: mixing waste beer yeast liquid and biodiesel byproducts, adding a carrier treated with the waste beer yeast liquid and the biodiesel byproducts, adding an enzyme preparation, mixing and reacting, and separating a supernatant to obtain a biocomposite carbon source; The preparation of the carrier comprises the following steps: a. Mix metakaolin with sodium hydroxide solution to form granules, then immerse them in biodiesel byproducts for 1-3 hours, continue to heat to 60-80 °C, and stand for 20-30 hours; take out the granules and calcine them at 400-550 °C for 2-4 hours to obtain precursor granules; b. Place the precursor particles in the biodiesel byproduct, first heat treat, and then react at 220-250 °C for 3-5 h in a nitrogen atmosphere; then cool to 20-40 °C, add beer waste yeast solution to the system to double the volume of the system, and after ultrasonic treatment for 0.1-1 h, take out the particles to obtain the carrier.

2. The method for preparing the biocomposite carbon source according to claim 1, characterized in that: The mass ratio of the waste beer yeast liquid to the biodiesel byproduct is 10:1-10; the dosage of the enzyme preparation is 0.5-5 wt%.

3. The method for preparing the biocomposite carbon source according to claim 1, characterized in that: The enzyme preparation is one or more of yeast wall-breaking enzyme, cellulase, xylanase, acid protease, beta-glucanase, alkaline protease, lipase, neutral protease and papain.

4. The method for preparing the biocomposite carbon source according to claim 1, characterized in that: The temperature of the mixed reaction is 10-50°C; the time of the mixed reaction is 20-80 hours.

5. The method for preparing the biocomposite carbon source according to claim 1, characterized in that: Before adding the enzyme preparation, adjust the pH of the system to 6~9.

6. The method for preparing a biocomposite carbon source according to claim 1, characterized in that: The mass ratio of the carrier to the biodiesel byproduct is 1:1-100; the mass ratio of the sodium hydroxide to the metakaolin in step a is 1:3-4.

7. The method for preparing a biocomposite carbon source according to claim 1, characterized in that: The heat treatment in step b is ultrasonic mixing at 50-70°C for 10-30 min.

8. A biocomposite carbon source prepared by the preparation method according to any one of claims 1 to 7, characterized in that: COD Cr The value is 100000~1000000 mg / L.

9. Use of the biological composite carbon source according to claim 8 in sewage denitrification treatment, characterized in that: The sewage includes one or more of domestic sewage, industrial sewage or garbage leachate.

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