Green deodorant antibacterial composition and application thereof

Abstract

The invention discloses a green deodorant bacteriostatic composition and application thereof, the green deodorant bacteriostatic composition is composed of a soapberry lactobacillus fermentation broth and a natural plant extract, and the mass ratio of the soapberry lactobacillus fermentation broth to the natural plant extract is (1-4): (4-1); the natural plant extract is composed of a rhamnus rhamnoides extract and a tree moss absolute oil extract; the mass ratio of the rhamnus rhamnoides extract to the tree moss absolute oil extract is (90-98): (2-10). The composition disclosed by the invention has an extremely strong antibacterial synergistic effect, has an inhibiting effect on fungi such as white candida and malassezia, can also inhibit streptococcus pneumoniae, bacillus influenza, catarrhalis and the like, and obviously improves antibacterial and broad-spectrum effects. And the composition has an excellent odor removal effect, and can be applied to personal care and household cleaning products.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and particularly relates to a green deodorizing and antibacterial composition and application thereof. Background Art

[0002] Daily chemical products are susceptible to microbial contamination during production, storage and use, which can lead to bacterial growth, corruption and deterioration of the products, and changes in their smell and color, such as product odor and darkening, which reduces or even makes the products lose their value. In order to prevent daily chemical products from causing harm to human skin and product deterioration and mold due to microbial contamination, the most effective way is to add preservatives, which are chemical agents that can inhibit the growth and reproduction of microorganisms.

[0003] Commonly used preservatives in traditional daily chemical products include alcohols (phenoxyethanol), parabens (methyl, ethyl, propyl, butyl, isopropyl and isobutyl paraben, etc.), formaldehyde releasers (imidazolidinyl urea, diazolidinyl urea, sodium hydroxymethylglycinate, etc.), iodopropynyl butylcarbamate (IPBC), a mixture of methylchloroisothiazolinone and methylisothiazolinone (kason), etc. Most of these preservatives are toxic to the human body and may even cause allergies and cause certain damage to the human body and skin. Therefore, there are strict controls on the amount of preservatives added to daily chemical products.

[0004] As consumers' awareness and requirements for preservatives become higher and higher, the concept of safe "low-additive" or even "no-additive" preservative products is becoming more and more popular among consumers. Green preservatives are also accompanied by the progress of scientific research, and the industry is also conducting more and more in-depth research on the performance of plant-based preservatives. There is still a long way to go before plant-based green preservatives that are non-irritating and highly safe can be fully popularized. Therefore, it is of great significance to develop a combination of plant-based antibacterial agents to meet the needs of products, markets and consumers. Summary of the invention

[0005] The purpose of this section is to summarize some aspects of embodiments of the present invention and to briefly introduce some preferred embodiments.

[0006] As one aspect of the present invention, the present invention provides a green deodorizing and antibacterial composition, wherein: the green deodorizing and antibacterial composition is composed of Lactobacillus sapindus fermentation broth and natural plant extracts, the mass ratio of the Lactobacillus sapindus fermentation broth and the natural plant extracts is (1-4): (4-1); the natural plant extracts are composed of Rhamnus medica extract and tree moss absolute oil extract; the mass ratio of the Rhamnus medica extract and the tree moss absolute oil extract is (90-98): (2-10).

[0007] As a preferred embodiment of the green deodorizing and antibacterial composition of the present invention, the mass ratio of the cascara extract to the moss absolute oil extract is (95-97): (3-5).

[0008] As a preferred embodiment of the green deodorizing and antibacterial composition described in the present invention: the preparation method of the Lactobacillus soapberry fermentation liquid is as follows: the soapberry fruit is extracted with water, then decolorized with a decolorizer to obtain a soapberry fruit extract, lactobacillus is added to the soapberry fruit extract, and after fermentation, the soapberry fruit fermentation liquid is obtained by filtering.

[0009] As a preferred solution of the green deodorizing and antibacterial composition of the present invention: the water extraction temperature is 60-90° C., and the solid-liquid ratio is 1:2-6.

[0010] As a preferred embodiment of the green deodorizing and antibacterial composition of the present invention: the preparation method of the buckthorn extract is to crush the fruit, bark, root or stem of buckthorn, soak it in water, filter it to obtain the buckthorn extract, concentrate it, and dry it to obtain the powdered buckthorn extract.

[0011] As a preferred solution of the green deodorizing and antibacterial composition of the present invention: the water-adding soaking has a solid-liquid ratio of 1:5-20 and the soaking lasts for 20-35 days.

[0012] As a preferred embodiment of the green deodorizing and antibacterial composition described in the present invention: the tree moss pure oil extract is prepared by drying and crushing the tree moss, extracting with ethanol, filtering and concentrating to obtain a tree moss extract, then dissolving and purifying with ethanol, precipitating impurities at low temperature, filtering the impurities and distilling to obtain the tree moss pure oil extract.

[0013] As a preferred solution of the green deodorizing and antibacterial composition of the present invention: the ethanol extraction time is 12 to 36 hours, and the solid-liquid ratio is 1:2 to 5; the low-temperature precipitation of impurities is the precipitation of impurities at -10 to 0°C.

[0014] Beneficial effects of the present invention: The present invention discloses a plant deodorizing composition, wherein the fermentation liquid of Lactobacillus sapindus contains yeast extract and organic acid, the extract of Rhamnus medica includes a variety of anthraquinone compounds, such as rhamnosin, aloe-rhamnosin and rhamnosin, etc., which have the effect of inhibiting bacterial reproduction on a variety of bacteria, and the tree moss absolute oil extract benefits from its rich aromatic hydrocarbons, alcohols, esters and other compounds, which give the tree moss absolute oil natural medicinal properties such as bactericidal, antiseptic and anti-inflammatory properties. Through the compound combination of the three, it is found that there is a strong antibacterial synergistic effect, which has an inhibitory effect on fungi such as white schizonepeta and Malassezia, and can also inhibit Streptococcus pneumoniae, influenza bacillus, catarrhalis, etc., and compared with a single raw material or a compound of two extracts, the antibacterial and broad-spectrum effects can be significantly improved. And the composition has an excellent deodorizing effect, and the plant antibacterial composition of the present invention is applied to personal care and household cleaning products, and has strong antibacterial, antiseptic and deodorizing effects. DETAILED DESCRIPTION

[0015] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with specific embodiments.

[0016] The preparation method of Lactobacillus sapindus fermentation liquid comprises the following steps: crushing the soapberry fruit, adding water to extract for 2N4h at 60-90 DEG C, with a solid-liquid ratio of 1:2-6 (mass ratio), filtering with a 1000-2000 mesh filter bag to obtain a crude extract, adding 30wt% hydrogen peroxide to the crude extract for decolorization, wherein 5-8wt% hydrogen peroxide solution is added to 1wt% of the crude extract, and obtaining a soapberry fruit extract after decolorization, inoculating lactobacillus strains in the extract, fermenting for 48-96h, sterilizing under high pressure, and filtering to obtain the Lactobacillus sapindus fermentation liquid.

[0017] The preparation method of buckthorn extract is as follows: crush the fruit, bark, root or stem of buckthorn, add deionized water, the solid-liquid ratio is 1:5-20, soak at room temperature for 20-35 days, filter with a 1000-2000 mesh filter bag to obtain liquid buckthorn extract, concentrate the extract and then send it into a spray dryer for spray drying into powder, crush the dried powder and mix it evenly to obtain powdered buckthorn extract.

[0018] Preparation method of tree moss absolute oil extract: dry the tree moss raw material, then crush it into powder with a particle size of about 20-60 mesh, add ethanol to extract for 12-36 hours, with a solid-liquid ratio of 1:2-5, and then filter out undissolved impurities with a 200-500 mesh filter bag, heat and concentrate the ethanol extract to remove the ethanol solvent to obtain the tree moss extract. Add 95% ethanol to the tree moss extract for re-dissolution, with a solid-liquid ratio of 1:1-3, so that the active ingredients in the tree moss extract are re-dissolved in the ethanol solvent. The re-dissolved solution is stored at low temperature (-10-0℃) for 24 hours to precipitate and precipitate the insoluble matter or impurities in the solution. Then filter out these insolubles and impurities to obtain a relatively pure solution. The filtered solution is molecularly distilled to obtain the tree moss absolute oil extract.

[0019] Embodiment 1:

[0020] Raw materials of the present invention:

[0021] Raw material 1: Lactobacillus sapindus fermentation broth, INCl name is Sapindus mukossi fruit extract and lactobacillus fermentation product (LACTOBACI LLUSFERMENT).

[0022] Lactobacillus soapberry fermented liquid is rich in a variety of active ingredients, such as yeast extract and organic acids, which give it excellent moisturizing, antioxidant and anti-inflammatory effects. Lactobacillus fermented liquid can also repair the skin barrier and enhance the skin's moisturizing ability, thereby improving the skin's elasticity and radiance. Lactobacillus soapberry fermented liquid has been widely used in cosmetics and skin care products. For example, it can be added to cleansing products to help clean the skin while maintaining the skin's moisture; it can also be used in toners to provide moisture locking and hydration; in creams, it can improve the skin's elasticity and radiance; and in masks, it can soothe the skin and relieve symptoms of dry and sensitive skin.

[0023] The preparation method of Lactobacillus mukorossi fermentation liquid is as follows: after crushing the soapberry fruit, adding deionized water at 70°C to extract for 4 hours, the solid-liquid ratio is 1:4 (mass ratio), and then filtering with a 2000 mesh filter bag to obtain a crude extract, adding 30%wt hydrogen peroxide to the crude extract for decolorization, wherein 1 weight part of the crude extract is added with 6 weight parts of hydrogen peroxide solution, decolorizing for 6 hours to obtain the soapberry fruit extract, and then inoculating the soapberry fruit extract with Lactobacillus plantarum strains, the strain inoculation concentration is about 2x1 0 8 CFU / mL, fermented at 37°C for 96 h and then sterilized by high pressure, and then the residual plant lactobacillus and impurities were filtered out with a 0.22 μm filter membrane to obtain the soapberry lactobacillus fermentation liquid.

[0024] Ingredient 2: Rhamnus rhamnoides extract

[0025] Rhamnus extract can effectively improve gastrointestinal digestion and promote the natural laws of the stomach, thereby achieving the effect of moisturizing the intestines and nourishing the skin. In addition, it also helps to expel acne, expel stool, and make the intestines cleaner. When the intestinal function is good, the appetite will also become better, the skin condition will also improve, and the mood will naturally become better. In clinical practice, Rhamnus extract is also considered to have anti-cancer and anti-tumor effects, and can assist in weight loss, bowel cleansing, detoxification, and tightening the abdomen. In the field of traditional Chinese medicine, Rhamnus extract is also used to treat symptoms such as rheumatism and heat toxins. Its bark or root bark has the effects of clearing heat and detoxifying, cooling blood and killing insects, and treating wind-heat itching. In addition, Rhamnus extract also has certain applications in the cosmetics industry. The active ingredients it contains can be used to make cosmetics with specific effects, such as improving skin conditions and enhancing skin elasticity.

[0026] The preparation method of buckthorn extract is as follows: crush the fruit, bark, root or stem of buckthorn, add deionized water, the solid-liquid ratio is 1:5-20, soak at room temperature for 20-35 days, filter with a 1000-2000 mesh filter bag to obtain liquid buckthorn extract, concentrate the extract and then send it into a spray dryer for spray drying into powder, crush the dried powder and mix it evenly to obtain powdered buckthorn extract.

[0027] Ingredient 3: Evernia Furfuracea absolute extract (INCI name: Evernia Furfuracea extract)

[0028] The main benefits of moss absolute oil extract are its unique aroma and skin care effects. Moss absolute oil extract has the natural green aroma of moss substances and a strong resinous scent, which is long-lasting and unique. In terms of skin care, it can moisturize, hydrate and soothe the skin, helping to improve the health of the skin.

[0029] Preparation method of tree moss absolute oil extract: dry the tree moss raw material, then crush it into powder, add ethanol to extract for 24 hours, the solid-liquid ratio is 1:2 (weight ratio), and then filter out undissolved impurities with a 200-mesh filter bag to obtain an ethanol extract, heat and concentrate the ethanol extract to remove the ethanol solvent to obtain a tree moss extract, add 95% ethanol to the tree moss extract, the solid-liquid ratio is 1:1 (weight ratio), and the effective ingredients in the tree moss extract are redissolved in the ethanol solvent, and the obtained solution is stored at low temperature (0°C) for 24 hours to precipitate and precipitate insoluble matter or impurities in the solution, and filter out these insolubles to obtain a relatively pure solution. The filtered solution is molecularly distilled to obtain a tree moss absolute oil extract.

[0030] Preparation of green deodorizing and antibacterial composition:

[0031] The prepared Rhamnus medica extract and the tree moss absolute oil extract are mixed in a mass ratio of 95:5 to obtain a plant extract composition. The prepared Lactobacillus sapindus fermentation liquid and the plant extract composition are compounded in a certain mass ratio, deionized water is added to 100%, and the mixture is stirred and dissolved uniformly to obtain a green deodorizing and antibacterial composition. The green deodorizing and antibacterial composition is composed of the components in Table 1 in terms of mass percentage, and each component in Table 1 is in mass percentage.

[0032] Table 1 Green deodorizing and antibacterial composition

[0033]

[0034] Inhibition rate test:

[0035] According to "QB / T 2738-2023 Evaluation Method for Antibacterial Effect of Daily Chemical Products 7.3", the antibacterial effect of the composition solutions of experimental groups I to IV in Table 1 was evaluated. The test microorganisms were: Escherichia coli, Staphylococcus aureus, Candida albicans, and Malassezia. The sample concentration was the original solution, the action time was 10 minutes, and the amount of recovered bacteria was about 1×10 5 CFU / mL.

[0036] The data records of the bacteria and fungi rate tests of the samples in Table 1 are shown in Table 2:

[0037] Table 2 Antibacterial rate of the compositions of experimental groups I to IV

[0038] Bacteria Type I II III IV E. coli rate 98.5 92.7 47.6 71.5 Staphylococcus aureus rate 97.8 92.4 45.1 69.7 Candida albicans rate 96.5 91.8 41.8 62.7 Malassezia rate 95.9 91.1 38.4 74.5

[0039] The test results in the above table show that the antibacterial rates of the Lactobacillus sapindus fermentation broth or the plant compound alone are low, while the antibacterial rates of the combination against the above-mentioned multiple bacterial species are all above 90%, producing good antibacterial effects. Therefore, it can be inferred that the antibacterial effect of the combination on bacteria has a synergistic effect.

[0040] Verification of synergistic effect:

[0041] According to the "Technical Specifications for Disinfection" (2002 Edition) 2.1.8.3 Minimum Concentration Determination Test (Agar Dilution Method) for minimum concentration test, take 1 μl (containing about 10 4 cfu / ml) bacterial suspension was inoculated on a plate containing an antibacterial (inhibitory) culture medium. The diameter of the bacterial liquid circle formed after inoculation was about 5mm to 8mm (the amount of bacteria in each spot was about 10 3 cfu), the test samples include individual raw materials and compound composition raw materials, and the minimum concentration (MIC) results are shown in Table 3:

[0042] Table 3 Minimum inhibitory concentration (MIC) test

[0043]

[0044] Note: The numbers in the table represent the minimum inhibitory concentration, and the unit is mass percentage.

[0045] As shown in Table 3, the minimum inhibitory concentration (MIC) test of the individual raw materials and the composite composition raw materials showed that the composite composition of the combination of Lactobacillus sapindus fermentation broth and the plant extract composition can significantly reduce the MIC, and the antibacterial effect of the composition is significantly enhanced.

[0046] The chessboard microdilution method was used to determine the antibacterial effects of Lactobacillus sapindus fermentation broth and a plant extract composition (a combination of Rhamnus medica extract and moss absolute oil extract) on four bacteria. The results were judged by FIC and showed four effects: synergistic, additive, antagonistic and irrelevant.

[0047] FIC=MIC A 组合 / MIC A 单独 +MIC B 组合 / MIC B 单独

[0048] Where, MIC A 单独 and MIC B 单独 MIC and MIC when A and B are used alone respectively. A 组合 and MIC B The combination of Lactobacillus sapindus fermented liquid and plant extract composition achieves the same effect as when the two substances are used alone. When the FIC index is less than 0.5, the two substances are synergistic; when the FIC index is 0.5-1, the two substances are additive; when the FIC index is greater than 1 and less than 2, the two substances are irrelevant; when the FIC index is greater than 2, the two substances are antagonistic. The experimental results are shown in Table 4.

[0049] Table 4

[0050]

[0051]

[0052] As shown in Table 4, the experimental results show that when the fermentation broth of Lactobacillus sapindus and the plant extract are in a ratio of 1:4 to 4:1, FlC is less than 0.5, indicating that the composition of the present invention has a synergistic inhibitory effect on Escherichia coli, Staphylococcus aureus, Candida albicans and Malassezia.

[0053] Deodorization performance test:

[0054] A 1% by mass aqueous solution of ammonia and a 1% by mass aqueous solution of hydrogen sulfide were mixed in a ratio of 1:1 to prepare an odor source composition, 1 mL of the odor source composition was added to 99 mL of the test sample prepared according to Table 6, and allowed to act for 10 minutes, and then 50 testers were selected to evaluate the deodorization effect through olfactory scoring. The evaluation grade standard is shown in Table 5.

[0055] Table 5

[0056] Rating scale Scoring Criteria Completely deodorizes Score ≥ 400 Significantly removes odors 300 < Score ≤ 400 Slight deodorization 100 < score ≤ 300 Unable to remove odor 0≤score≤100

[0057] Table 6

[0058]

[0059] Note: Comparative group 6 is a blank control, i.e. distilled water is used instead of the test sample, and the amount of odor source added is the same as that of the experimental group. The formula data in the table are mass percentages.

[0060] Deodorization test results:

[0061] Table 7

[0062] Experiment number Example 1 Example 2 Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Score 438 427 417 255 296 0 Rating scale Completely deodorizes Completely deodorizes Completely deodorizes Slight deodorization Slight deodorization Unable to remove odor

[0063] The experimental results show that the combination of Lactobacillus soapberry fermentation broth and plant extracts can significantly remove the odors of ammonia and hydrogen sulfide.

[0064] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A green deodorizing and antibacterial composition, characterized in that: The green deodorizing and antibacterial composition is composed of Lactobacillus sapindus fermentation broth and natural plant extracts, the mass ratio of the Lactobacillus sapindus fermentation broth and the natural plant extracts is (1-4):(4-1); the natural plant extracts are composed of Rhamnus medica extract and tree moss absolute oil extract; the mass ratio of the Rhamnus medica extract and the tree moss absolute oil extract is (90-98):(2-10).

2. The green deodorizing and antibacterial composition according to claim 1, characterized in that: The mass ratio of the cascara officinalis extract to the moss absolute oil extract is (95-97): (3-5).

3. The green deodorizing and antibacterial composition according to claim 1 or 2, characterized in that: The preparation method of the Lactobacillus soapberry fermentation liquid is as follows: extracting soapberry fruit with water, decolorizing with a decolorizer to obtain a soapberry fruit extract, adding lactobacillus to the soapberry fruit extract, and filtering after fermentation to obtain the soapberry fruit fermentation liquid.

4. The green deodorizing and antibacterial composition according to claim 3, characterized in that: The water extraction temperature is 60-90° C., and the solid-liquid ratio is 1:2-6.

5. The green deodorizing and antibacterial composition according to claim 1 or 2, characterized in that: The preparation method of the buckthorn extract comprises the following steps: crushing the fruit, bark, root or stem of buckthorn, soaking in water, filtering to obtain buckthorn extract solution, concentrating and drying to obtain powdered buckthorn extract.

6. The green deodorizing and antibacterial composition according to claim 5, characterized in that: The water-adding soaking has a solid-liquid ratio of 1:5-20 and the soaking lasts for 20-35 days.

7. The green deodorizing and antibacterial composition according to claim 1 or 2, characterized in that: The tree moss absolute oil extract is obtained by drying and crushing the tree moss, extracting with ethanol, filtering and concentrating to obtain a tree moss extract, dissolving and purifying with ethanol, precipitating impurities at low temperature, filtering the impurities and distilling to obtain the tree moss absolute oil extract.

8. The green deodorizing and antibacterial composition according to claim 7, characterized in that: The ethanol extraction time is 12 to 36 hours, and the solid-liquid ratio is 1:2 to 5; the low-temperature impurity precipitation is the precipitation of impurities at -10 to 0°C.

9. Use of the natural plant deodorizing and antibacterial composition according to claim 1 in antibacterial daily chemical products.

10. The use according to claim 9, characterized in that: The daily chemical products include hand soap, laundry detergent or dishwashing liquid.

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