Equipment for detecting genes of odor substances in water on site

Abstract

The invention relates to the technical field of detection equipment, in particular to equipment for detecting genes of odor substances in water on site. The device comprises a sampling system and a suction filtration system, the sampling system comprises a mechanical arm, the suction filtration system comprises a suction filtration platform, the mechanical arm is installed on the suction filtration platform, a sampling device is installed at the bottom of the mechanical arm, at least four first through holes are symmetrically formed in the suction filtration platform, and suction filtration devices are installed in the first through holes; according to the device disclosed by the invention, accurate collection of samples and uploading of sample information are completed through the sampling system and the suction filtration system of the device, so that information such as sampling positions is more accurate, the labor cost is greatly saved, and the timeliness of the samples is improved; through the mechanical arm of the fluorescent quantitative PCR system of the device, accurate preparation of the quantitative PCR system is realized, and proper primers are selected, so that manual errors are reduced, and powerful guarantee is provided for in-situ recognition of olfactory genes and early warning of odor risks.

Description

Technical Field

[0001] The present invention relates to the technical field of detection equipment, and in particular to a device for on-site detection of genes of odor substances in water. Background Art

[0002] The water flow rate in lake-reservoir water sources is slow, the self-purification capacity of the water body is weak, there are many types of odor-producing algae, and some algae species are similar in morphology. At present, the identification of odor-producing algae mainly adopts the traditional microscopic examination method, and the traditional detection methods for odor substances are sensory analysis and joint analysis. Sensory analysis methods include flavor rating scale (FRS), threshold odor number (TON) and flavor profile analysis (FPA). The advantage of sensory analysis is that it can comprehensively perceive substances in water. Commonly used instrumental analysis methods include electronic nose measurement, gas chromatography, and gas chromatography-mass spectrometry, among which gas chromatography-mass spectrometry is the most widely used. With the development of high-sensitivity and high-accuracy chromatographic analysis methods, GC-MS is usually used to identify and detect odor substances.

[0003] Since the concentration of odor substances in the environment is extremely low, pretreatment technology is usually used before instrumental analysis to improve the accuracy and sensitivity of qualitative and quantitative analysis. The main pretreatment technologies include closed-circuit gas stripping analysis (CLSA), resin adsorption (RA), liquid-liquid extraction (LLE), solid phase extraction (SPE), solid phase microextraction (SPME), stir bar adsorption extraction (SBSE), liquid phase microextraction (LPME), purge and trap (P&T) and distillation extraction (SDE). Since the pretreatment of solid phase microextraction (SPME) is simple to operate, does not consume organic reagents, and requires a small amount of sample, it is currently the most commonly used pretreatment method for odor substance analysis. The SPME pretreatment method combined with GC-MS instrumental analysis can meet the requirements of qualitative and quantitative analysis of odor substances.

[0004] At present, water samples are mainly collected manually. The time interval between sample collection and use is long, the properties of the samples may change, and the timeliness is poor. At present, the identification of odor-producing algae mainly adopts microscopic examination, which is time-consuming and labor-intensive, has low accuracy, has certain errors, and cannot distinguish between odor-producing and non-odor-producing cyanobacteria in terms of morphology. From the perspective of traditional detection methods for odorous substances, sensory analysis methods are highly subjective, and there are large errors in odor evaluation, and different people have large differences in odor evaluation. Instrumental analysis methods also have problems such as complex operation, low concentration of environmental samples, and difficulty in determining structural analysis. Therefore, it is difficult to carry out on-site detection and traceability of odor-causing substances.

[0005] The prior art provides some solutions, such as patent EP282332B1, which provides a method for detecting the smell of substances or other odors, wherein the method includes adsorbing the sample into a device with a double-layer membrane, and detecting the trace odor substance genes contained in the sample by measuring the change in membrane potential or resistance caused by the adsorption. This invention has made significant progress in the rapid detection of trace odor substance genes contained in samples, but it still has some shortcomings in the tracing of odor substances. Here, the inventor believes that there is still a lot of room for improvement. Summary of the invention

[0006] In order to realize the collection of water samples through equipment, reduce the cost of manual collection, and improve the timeliness of algae samples in the water; provide a simple and effective way to extract DNA on site, use real-time fluorescence quantitative PCR method to detect the olfactory potential of algae in the water, warn of odor outbreaks, and accurately quantify the technical effect of odor-producing algae.

[0007] The present invention provides a device for on-site detection of genes of odorous substances in water, the device comprises a sampling system and a filtration system, the sampling system comprises a mechanical arm, the filtration system comprises a filtration platform, the mechanical arm is installed on the filtration platform, a sampling device is installed at the bottom of the mechanical arm, at least four first through holes are symmetrically opened on the filtration platform, a filtration device is installed in the first through holes, and a filtration tube is connected to the bottom of the filtration device.

[0008] Furthermore, the present invention can collect accurate volume water samples on site through a sampling system, filter the collected water samples, and complete DNA enrichment. The filtered filter membrane is marked with a QR code to record the sampling time, longitude and latitude, etc., which improves the accuracy of sample collection and pre-processing.

[0009] In the present invention, the mechanical arm is arranged on the symmetric midline of the first through hole, a nozzle is arranged at the end of the mechanical arm, and the mechanical arm can be rotated to align the nozzle with the first through hole.

[0010] Furthermore, the sampling device connected to the bottom of the robotic arm can quantitatively collect raw water from lakes or reservoirs, and discharge the collected raw water through a nozzle arranged at the end to a filtration device in the first through hole. The filtration device filters the collected water samples and enriches the raw water DNA on the filter membrane of the first filter disk. Finally, the filtered filter membrane is packaged and marked with QR code information for storage through a QR code information marking device.

[0011] It is worth mentioning here that the main steps of the entire detection process include: enriching the algae in the actual water body through a membrane; eluting the enriched sample; extracting the nucleic acid of the sample DNA; setting up a fluorescent quantitative PCR system equipped with equipment to conduct quantitative detection; outputting the detection data and calculating the concentration of the olfactory gene to draw the final conclusion.

[0012] The specific structure of the fluorescence quantitative PCR system equipment device is as follows: the fluorescence quantitative PCR system equipment device is rectangular in shape, including a sample area, a sample area to be tested, a gun tip placement area, a 96-well plate area, a standard sample area, and a multi-linked robotic arm. The sample area is in the upper left corner of the device, used to place DNA samples to be processed; the gun tip placement area is in the upper right corner of the device, where pipette tips of different specifications are placed; the 96-well plate area is in the lower right corner of the device, responsible for equipping the fluorescence quantitative PCR system and mixing the system.

[0013] In addition, the specific operation steps can also be detailed as follows: firstly, collect a water sample of the water body to be evaluated, filter the water sample and collect the sample on the filter membrane after filtration to obtain the sample to be evaluated; Then, a DNA extraction kit is used to extract the DNA of the sample to be evaluated to obtain the DNA of the sample to be evaluated; Then, the DNA of the sample to be evaluated was used as a template to perform real-time fluorescence quantitative PCR to obtain the Cq value of the water body to be evaluated; Then, the mic gene concentration N in the water body to be evaluated is calculated according to the standard curve A of the mic gene copy number concentration and the Cq value of real-time fluorescence quantitative PCR; The experimental calculations here will not be described in detail in the present invention, and reference may be made to patent CN113943782A for details.

[0014] In the present invention, the filtration device includes a filtration matrix, which is a hollow structure, and a first filter sleeve with an inclined wall extending downward from its upper end, a through hole is opened in the center of the first filter sleeve and a first filter disc is arranged.

[0015] Furthermore, the filtration device used in the present invention mainly uses vacuum filtration technology, which can enrich the raw water DNA in the collected water sample on the filter membrane of the first filter disc. In addition, the first filter sleeve in the filtration matrix is ​​arranged inside the filtration matrix, and the first filter sleeve is inclined, so that the water sprayed by the upper mechanical arm can directly enter the first filter sleeve and be filtered through the first filter disc. Since the side wall surface of the first filter sleeve is inclined, the probability of the sprayed water falling to the outside of the upper end of the filtration matrix can be reduced, and the inclined wall surface can make the water body be discharged downward in a more concentrated manner, thereby improving the overall filtration effect.

[0016] In the present invention, a plurality of filter holes are provided on the first filter disc, a first spring is installed at the bottom of the first filter disc, an auxiliary filter assembly is connected to the other end of the first spring, a second filter sleeve is sleeved on the outside of the auxiliary filter assembly, and the side of the second filter sleeve does not abut against the inner wall of the filtration base.

[0017] Furthermore, the wall structure of the second filter sleeve is similar to that of the first filter sleeve and is also inclined, which can also enable the water body to be gathered in the middle of the filter sleeve, so that the water body can be discharged downward in a more concentrated manner, thereby improving the filtration effect.

[0018] In the present invention, the side of the second filter sleeve is sleeved with an extension ring, the lower end surface of the extension ring is provided with a downwardly extending insertion rod, the outer side of the insertion rod is sleeved with a second spring, the other end of the second spring is connected to an annular block, and the annular block is fixedly connected to the inner wall surface of the filtration matrix. Furthermore, the first filter sleeve and the second filter sleeve can realize multi-stage filtration, thereby increasing the amount of sample DNA obtained by a single filtration.

[0019] Furthermore, by designing components such as the plug rod and the second spring, the impact force of the water on the second filter disc during the filtration process can be adjusted. Since the water falls downward into the second filter sleeve after passing through the first filter disc, the water has an impact force on the second filter sleeve. If the impact force is too large, the second filter sleeve is compressed so that the extension ring on its side is synchronously displaced downward, thereby causing the second spring to deform. When the impact pressure of the water becomes smaller, the second spring is reset to control the upward displacement of the second filter sleeve. Through the action of the spring, the impact effect of the water on the second filter sleeve is relatively reduced, thereby reducing the probability of the filter membrane coated on the outer side of the second filter disc on the auxiliary filter assembly installed at the bottom of the second filter sleeve being deformed by the impact, thereby ensuring the effective filtering aperture of the filter membrane and the overall deformation range of the second filter sleeve. In addition, the design of the second spring and the extension ring can also adjust the speed at which the water discharged from the first filter sleeve enters the second filter sleeve and is discharged downward from the second filter sleeve, that is, regulating the speed of discharge from the filtration tube during the entire filtration process. On the other hand, by driving the second filter sleeve to move up and down by the second spring, the angle of action of the water discharged from the first filter sleeve impacting the second filter sleeve can be changed. Under the impact of the water, the second spring is deformed, and there is a gap between the extension ring and the wall of the filtration matrix. In this way, the second filter sleeve can produce horizontal displacement or axial tilt under the impact, so as to change the angle and range of the water flow impacting downward from the first filter sleeve on the second filter sleeve, avoid excessive concentration of samples on the filter membrane due to continuous impact on one position, and avoid the occurrence of problems such as squeezing of samples obtained by filtration and enrichment and reduced activity.

[0020] In the present invention, the auxiliary filter assembly includes an annular connecting sleeve, the upper and lower inner end surfaces of the connecting sleeve are fixedly connected to at least three support bars, the other ends of the support bars are commonly connected to a circular plate, and one end of the circular plate is fixedly connected to the first spring.

[0021] In the present invention, the second filter disc is connected to the inner wall surface formed by the fixed connection between the support bar and the circular plate, and a protrusion is arranged on the outer side of the connecting sleeve, and the protrusion can be installed in cooperation with the inner wall of the second filter sleeve.

[0022] Furthermore, the auxiliary filter assembly can realize secondary filtration and improve the enrichment efficiency of a single water sample. At the same time, since the support bar is made of memory alloy, when water flows into the auxiliary filter assembly through the first filter sleeve, if the water has a certain impact force, the support bar is deformed downward by force, thereby changing the angle and range of the water flow from the first filter sleeve to the second filter sleeve, thereby avoiding continuous impact on the same filter position, resulting in damage to the enriched sample. The auxiliary filter assembly includes a circular plate connected to the first filter disc through a first spring, and a protrusion installed in cooperation with the inner wall of the second filter sleeve, so that the auxiliary filter assembly can slide relative to the second filter sleeve when it is impacted by water, thereby reducing the possibility of damage to the auxiliary filter assembly and improving the service life of the auxiliary filter assembly.

[0023] Compared with the prior art, the significant progress of the present invention is that the present invention can provide a device for in-situ sampling and DNA enrichment and automatic quantitative PCR, which can solve the technical problems that the existing reservoir sampling and DNA enrichment have high labor costs, the detection process of odor-causing substances MIB and geosmin requires large instruments, and the instrument analysis and detection process is long, the sensitivity is low, the detection limit is poor, and the quantitative PCR system has large manual errors. The present invention uses the sampling system and the filtration system of the device to complete the accurate collection of samples and the uploading of sample information, so that information such as the sampling location is more accurate, greatly saving labor costs and improving the timeliness of samples; the mechanical arm of the fluorescence quantitative PCR system of the device is used to realize the precise configuration of the quantitative PCR system, and select appropriate primers to reduce manual errors, providing a strong guarantee for in-situ identification of olfactory genes and odor risk warning. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the structure of a device for on-site detection of genes of odor substances in water involved in the present invention; Figure 2 This is a schematic structural diagram from another perspective of a device for on-site detection of genes of odor substances in water involved in the present invention; Figure 3 It is a schematic diagram of the structure of the suction filtration device involved in the present invention; Figure 4 This is a schematic diagram of the structure of the auxiliary filtering assembly involved in the present invention; Figure 5 This is a schematic diagram of the structure of the filtration device after the collection assembly is installed according to the present invention; Figure 6 This is a schematic diagram of the structure of the collection component involved in the present invention; Figure 7 It is a schematic diagram of the connection structure between the collecting plate and the collecting cover involved in the present invention.

[0025] Explanation of the reference numerals: 1-sampling system; 11-mechanical arm; 12-sampling device; 13-nozzle; 2-filtration system; 21-filtration platform; 22-first through hole; 23-filtration device; 231-filtration matrix; 232-first filter sleeve; 233-first filter disc; 234-first spring; 235-second filter sleeve; 236-extension ring; 237-insertion rod; 238-second spring; 239-annular block; 3-auxiliary filter assembly; 31-connecting set; 32-support bar; 33-circular plate; 34-second filter disc; 35-protrusion; 4-collecting assembly; 41-collecting cover; 42-connecting plate; 43-swinging plate; 44-third spring; 45-collecting plate. DETAILED DESCRIPTION

[0026] Embodiment 1: Reference Figure 1 , Figure 2 As shown, the present invention provides a device for on-site detection of genes of odorous substances in water, the device includes a sampling system 1 and a filtration system 2, the sampling system 1 includes a mechanical arm 11, the filtration system 2 includes a filtration platform 21, the mechanical arm 11 is installed on the filtration platform 21, a sampling device 12 is installed at the bottom of the mechanical arm 11, at least four first through holes 22 are symmetrically opened on the filtration platform 21, a filtration device 23 is installed in the first through hole 22, and a filtration tube 24 is connected to the bottom of the filtration device 23.

[0027] Furthermore, the present invention can collect accurate volume water samples on site through the sampling system 1, and filter the collected water samples to enrich DNA. The filtered filter membrane is marked with a QR code to record the sampling time, longitude and latitude, etc., thereby improving the accuracy of sample collection and pre-processing.

[0028] In the present invention, the mechanical arm 11 is disposed on the symmetrical midline of the first through hole 22 , and a nozzle 13 is disposed at the end of the mechanical arm 11 . The mechanical arm 11 can be rotated to align the nozzle 13 with the first through hole 22 .

[0029] Furthermore, the sampling device 12 connected to the bottom of the robotic arm 11 can quantitatively collect raw water from lakes or reservoirs, and discharge the collected raw water through the nozzle 13 arranged at the end to the suction filtration device 23 in the first through hole 22. The suction filtration device filters the collected water sample and enriches the raw water DNA on the filter membrane of the first filter disk 233. Finally, the filtered filter membrane is packaged and marked with QR code information through the QR code information labeling device for storage.

[0030] It is worth mentioning here that the main steps of the entire detection process include: enriching the algae in the actual water body through a membrane; eluting the enriched sample; extracting the nucleic acid of the sample DNA; setting up a fluorescent quantitative PCR system equipped with equipment to conduct quantitative detection; outputting the detection data and calculating the concentration of the olfactory gene to draw the final conclusion.

[0031] The specific structure of the fluorescence quantitative PCR system equipment device is as follows: the fluorescence quantitative PCR system equipment device is rectangular in shape, including a sample area, a sample area to be tested, a gun tip placement area, a 96-well plate area, a standard sample area, and a multi-linked robotic arm. The sample area is in the upper left corner of the device, used to place DNA samples to be processed; the gun tip placement area is in the upper right corner of the device, where pipette tips of different specifications are placed; the 96-well plate area is in the lower right corner of the device, responsible for equipping the fluorescence quantitative PCR system and mixing the system.

[0032] In addition, the specific operation steps can also be detailed as follows: firstly, collect a water sample of the water body to be evaluated, filter the water sample and collect the sample on the filter membrane after filtration to obtain the sample to be evaluated; Then, a DNA extraction kit is used to extract the DNA of the sample to be evaluated to obtain the DNA of the sample to be evaluated; Then, the DNA of the sample to be evaluated was used as a template to perform real-time fluorescence quantitative PCR to obtain the Cq value of the water body to be evaluated; Then, the mic gene concentration N in the water body to be evaluated is calculated according to the standard curve A of the mic gene copy number concentration and the Cq value of real-time fluorescence quantitative PCR; The experimental calculations here will not be described in detail in the present invention, and reference may be made to patent CN113943782A for details.

[0033] In the present invention, the filtration device 23 includes a filtration base 231, which is a hollow structure, and a first filter sleeve 232 with an inclined wall extends downward from its upper end. A through hole is opened in the center of the first filter sleeve 232 and a first filter disc 233 is provided.

[0034] Furthermore, the filtration device 23 used in the present invention mainly uses vacuum filtration technology, which can enrich the raw water DNA in the collected water sample on the filter membrane of the first filter disc 233. In addition, the first filter sleeve 232 in the filtration matrix 231 is arranged inside the filtration matrix 231, and the first filter sleeve 232 is inclined, so that the water sprayed by the upper mechanical arm 11 can directly enter the first filter sleeve 232 and be filtered through the first filter disc 233. Since the side wall surface of the first filter sleeve 232 is inclined, the probability of the sprayed water falling to the outside of the upper end of the filtration matrix 231 can be reduced, and the inclined wall design can make the water body be discharged downward in a more concentrated manner, thereby improving the overall filtration effect.

[0035] Embodiment 2: Reference Figure 1 , Figure 2 , Figure 3 , Figure 4 As shown, in the present invention, a plurality of filter holes are provided on the first filter disc 233, a first spring 234 is installed at the bottom of the first filter disc 233, the other end of the first spring 234 is connected to the auxiliary filter assembly 3, a second filter sleeve 235 is sleeved on the outside of the auxiliary filter assembly 3, and the side of the second filter sleeve 235 does not abut against the inner wall of the filtration base 231.

[0036] Furthermore, the wall structure of the second filter sleeve 235 is similar to that of the first filter sleeve 232 and is also inclined, which can also enable the water body to be gathered in the middle of the filter sleeve, so that the water body can be discharged downward in a more concentrated manner, thereby improving the filtration effect.

[0037] In the present invention, the side of the second filter sleeve 235 is sleeved with an extension ring 236, the lower end surface of the extension ring 236 is provided with a downwardly extending plug rod 237, the outer side of the plug rod 237 is sleeved with a second spring 238, the other end of the second spring 238 is connected to an annular block 239, and the annular block 239 is fixedly connected to the inner wall surface of the filtration matrix 231. Furthermore, the first filter sleeve 232 and the second filter sleeve 235 can realize multi-stage filtration, thereby increasing the amount of sample DNA obtained by a single filtration.

[0038] Furthermore, by designing the insert rod 237 and the second spring 238 and other components, the impact force of the water on the second filter disc 34 during the filtration process can be adjusted. Since the water falls downward into the second filter sleeve 235 after passing through the first filter disc 233, the water has an impact force on the second filter sleeve 235. If the impact force is too large, the second filter sleeve 235 is compressed so that the extension ring 236 on its side is synchronously displaced downward, thereby causing the second spring 238 to deform. When the impact pressure of the water becomes smaller, the second spring 238 is reset to control the second filter sleeve 235 to move upward, so that the spring can make the second filter sleeve 235 move upward. The impact effect of water on the second filter sleeve 235 is relatively reduced, which can reduce the probability of the filter membrane coated on the outside of the second filter disc 34 on the auxiliary filter assembly 3 installed at the bottom of the second filter sleeve 235 being deformed by impact, thereby ensuring the effective filtering aperture of the filter membrane and the overall deformation range of the second filter sleeve 235; in addition, the design of the second spring 238 and the extension ring 236 can also adjust the speed at which the water discharged from the first filter sleeve 232 enters the second filter sleeve 235 and is discharged downward from the second filter sleeve 235, that is, regulate the speed of water discharged from the suction tube during the entire filtration process. On the other hand, by driving the second filter sleeve 235 to move up and down by the second spring 238, the angle of action of the water discharged from the first filter sleeve 232 impacting the second filter sleeve 235 can be changed. Under the impact of the water, the second spring 238 is deformed, and there is a gap between the extension ring 236 and the wall of the filtration matrix 231, so that the second filter sleeve 235 can produce horizontal displacement or axial tilt under the impact, so as to change the angle and range of the water flow impacting downward from the first filter sleeve 232 acting on the second filter sleeve 235, avoid excessive concentration of samples on the filter membrane due to continuous impact on one position, and avoid the occurrence of problems such as squeezing of samples obtained by filtration and enrichment and reduced activity.

[0039] In the present invention, the auxiliary filter assembly 3 includes an annular connecting sleeve 31, and the upper and lower inner end surfaces of the connecting sleeve 31 are fixedly connected to at least three support bars 32, and the other ends of the support bars 32 are commonly connected to a circular plate 33, and one end of the circular plate 33 is fixedly connected to the first spring 234.

[0040] In the present invention, the second filter disc 34 is connected to the inner wall surface formed by the fixed connection between the support bar 32 and the circular plate 33, and a protrusion 35 is provided on the outer side of the connecting sleeve 31, and the protrusion 35 can be installed in cooperation with the inner wall of the second filter sleeve 235.

[0041] Furthermore, the auxiliary filter assembly 3 can realize secondary filtration and improve the enrichment efficiency of a single water sample. At the same time, since the support bar 32 is made of memory alloy, when water flows into the auxiliary filter assembly 3 through the first filter sleeve 232, if the water has a certain impact force, the support bar 32 is forced to deform downward, thereby changing the angle and range of the water flow of the first filter sleeve 232 impacting downward on the second filter sleeve 235, avoiding continuous impact on the same filtering position to cause the enriched sample to be damaged, etc. The auxiliary filter assembly 3 includes a circular plate 33 connected to the first filter disc 233 through the first spring 234 and a protrusion 35 installed in cooperation with the inner wall of the second filter sleeve 235, so that the auxiliary filter assembly 3 can slide relative to the second filter sleeve 235 when it is impacted by water, reducing the possibility of damage to the auxiliary filter assembly 3 and improving the service life of the auxiliary filter assembly 3.

[0042] Embodiment 3: Reference Figure 5 , Figure 6 , Figure 7 As shown, a collecting assembly 4 is sleeved on the upper end of the filtration base 231, and the collecting assembly includes a conical collecting cover 41, and a plurality of connecting plates 42 are arranged around the inner surface of the collecting cover 41. A swing plate 43 is hinged at the end of the connecting plate 42, and the other end of the swing plate 43 is connected to the surface of the collecting cover 41 through a third spring 44. At the same time, a collecting plate 45 is fixedly connected to the surface of the swing plate 43, and the collecting plate 45 is parallel to the swing plate 43.

[0043] Furthermore, since the nozzle of the robot arm 11 sprays water downward, there is a possibility that the water will splash onto the outside of the suction filter device 23, so the collecting assembly 4 is provided to collect the splashed samples and guide them to flow back into the suction filter device 23. Since the third spring 44 is provided on the rear side of the swing plate 43 and the collecting plate 45, if the robot arm 11 is not completely aligned with the center of the suction filter device 23, the water ejected from the nozzle 13 will impact the collecting assembly 4. After the splashed water contacts the collecting plate 45, the collecting plate 45 will first deform and push back, and then the water will return to the center range of the collecting cover 41 and further flow into the first filter sleeve 232. In this way, the water after the push back has a probability of contacting the splashed water, so as to reduce the splashing range of the fluid when the robot arm 11 sprays.

[0044] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

Claims

1. A device for on-site detection of odorous substance genes in water, characterized in that: The device comprises a sampling system (1) and a filtration system (2), wherein the sampling system (1) comprises a mechanical arm (11), and the filtration system (2) comprises a filtration platform (21). The mechanical arm (11) is mounted on the filtration platform (21), and a sampling device (12) is mounted at the bottom of the mechanical arm (11). At least four first through holes (22) are symmetrically provided on the filtration platform (21), and a filtration device (23) is mounted in each of the first through holes (22). The bottom of the filtration device (23) is connected to a filtration pipe (24).

2. The device for on-site detection of odorous substance genes in water according to claim 1, characterized in that: The mechanical arm (11) is arranged on the symmetrical midline of the first through hole (22); a nozzle (13) is arranged at the end of the mechanical arm (11); and the mechanical arm (11) can be rotated so that the nozzle (13) is aligned with the first through hole (22).

3. The device for on-site detection of odorous substance genes in water according to claim 1, characterized in that: The suction filtration device (23) comprises a suction filtration base (231), the suction filtration base (231) being a hollow structure, a first filter sleeve (232) with an inclined wall extending downward from an upper end thereof, a through hole being provided at the center of the first filter sleeve (232) and being provided with a first filter disc (233).

4. The device for on-site detection of odorous substance genes in water according to claim 3, characterized in that: The first filter disc (233) is provided with a plurality of filter holes, a first spring (234) is installed at the bottom of the first filter disc (233), the other end of the first spring (234) is connected to an auxiliary filter assembly (3), a second filter sleeve (235) is sleeved on the outside of the auxiliary filter assembly (3), and the side surface of the second filter sleeve (235) does not abut against the inner wall of the filtration base (231).

5. The device for on-site detection of odorous substance genes in water according to claim 4, characterized in that: An extension ring (236) is sleeved on the side surface of the second filter sleeve (235); a downwardly extending insertion rod (237) is provided on the lower end surface of the extension ring (236); a second spring (238) is sleeved on the outer side of the insertion rod (237); the other end of the second spring (238) is connected to an annular block (239); and the annular block (239) is fixedly connected to the inner wall surface of the filtration base (231).

6. The device for on-site detection of odorous substance genes in water according to claim 4, characterized in that: The auxiliary filter assembly (3) comprises an annular connection sleeve (31), the upper and lower inner end surfaces of the connection sleeve (31) being fixedly connected to at least three support bars (32), the other ends of the support bars (32) being commonly connected to a circular plate (33), and one end of the circular plate (33) being fixedly connected to the first spring (234).

7. The device for on-site detection of odorous substance genes in water according to claim 6, characterized in that: A second filter disc (34) is connected to an inner wall surface formed by the fixed connection between the support bar (32) and the circular plate (33), and a protrusion (35) is provided on the outer side of the connecting sleeve (31), and the protrusion (35) can be mounted in cooperation with the inner wall of the second filter sleeve (235).

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