Fat cutting filter, fat cutting filtering method and fat cutting filtering device

Abstract

The invention relates to a fat cutting filter, a fat cutting filtering method and a fat cutting filtering device.The fat cutting filter comprises a shell, the interior of the shell is a treatment cavity, and the shell is provided with a first connector and a second connector which communicate with the treatment cavity; the cutting part is located in the treatment cavity and located between the first connector and the second connector, the cutting part comprises a rigid silk screen used for cutting fat, and the silk diameter of the rigid silk screen ranges from 0.05 mm to 1 mm. The mesh wires of the rigid silk screen are used for fat cutting, the structure is simple, operation is easy, convenient and efficient, the machining cost is low, the mesh diameter can be customized according to needs, the porosity can reach 90% or above, the cutting efficiency is high, and the problem of filtering blockage during fat cutting can be effectively solved.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a fat cutting filter, a fat cutting filtering method, and a fat cutting filtering device. Background Art

[0002] Vascular stromal fraction (SVF) is derived from mature adipose tissue and is a heterogeneous group of cells, including adipose-derived stem cells (ADSCs), endothelial progenitor cells (EPCs), hematopoietic stem cells (HPCs), etc. Studies have reported that ADSCs obtained by SVF adherent culture in vitro can differentiate into osteocytes, chondrocytes, adipocytes, etc. under different induction environments; and literature shows that SVF can secrete a large amount of angiogenic growth factors and can differentiate into endothelial cells to participate in vascular sprouting.

[0003] Autologous fat transplantation refers to a tissue transplantation technique in which autologous fat tissue obtained from the donor area through surgical excision or negative pressure aspiration is transplanted to the recipient area after certain processing in vitro (including rinsing, cutting, centrifugation, purification, adding autologous stem cells or allogeneic cytokines, drugs, etc.) in order to increase tissue volume, promote tissue regeneration or improve tissue texture.

[0004] Adipose tissue has been considered an endocrine organ due to its role in energy regulation, inflammatory response, and immune response. In addition, it also has multipotent cells with multidirectional differentiation potential, which are present in the stromal vascular fraction (SVF) of adipose tissue.

[0005] The stromal vascular fraction (SVF) in adipose tissue is a group of multiphase cell populations obtained after the mature adipocytes are removed by filtration and centrifugation. Because SVF is easy to extract from adipose tissue and contains rich, highly plastic, and multidirectionally differentiated adipose tissue-derived mesenchymal stem cells (ADSCs), SVF can be directly used to treat inflammation and pain in osteoarthritis, and can also be used to improve facial fat atrophy. Currently, many clinical trials are actively recruiting volunteers to evaluate the effects of SVF in treating related diseases, so revealing the cellular composition of SVF and clarifying its corresponding biological functions is of great significance.

[0006] SVF cells represent a heterogeneous group of cells that surround adipocytes in adipose tissue. SVF obtained from human breast and visceral adipose tissue contains endothelial cells, uncharacterized stromal cells, blood cells, and tissue macrophages. In addition, SVF is also a source of hematopoietic progenitor cells and endothelial stem cells.

[0007] SVF is usually prepared by enzymatic decomposition, mechanical separation and other methods. Traditional enzymatic digestion is mainly performed manually in an open environment using centrifuge tubes and pipetting equipment. The operation steps are complicated, and the qualifications of the operators are high. The operation process causes large cell loss and poses a risk of contamination. The mechanical method has gained clinical favor due to its simple operation and rapid preparation. The current mechanical separation and preparation method on the market is mainly to extract adipose tissue from patients or animals, remove the fat cells by cutting, filtering and centrifuging, and separate the SVF of the adipose tissue.

[0008] For example, adipose tissue is obtained by suction using a liposuction needle or liposuction equipment, and then transferred to a cutting and crushing device to crush the adipose tissue. After crushing, it is transferred to a centrifuge tube for centrifugal separation to obtain the effective substances that need to be collected in the end, which are mainly cell clusters other than fat cells - SVF (adipose-derived stem cells, endothelial progenitor cells, etc.) and extracellular matrix, which play an important role in tissue regeneration.

[0009] When the artificial cutting and filtration method is selected, a cutting filter containing one or more cutting filters is required. Two syringes are connected to both ends of the filter for repeated pushing and pulling. In the process of fat tissue passing through the pores on the cutting filter, larger mature fat cells are broken by squeezing and shearing, thereby completing the cutting and filtration without causing damage to the smaller SVF cells.

[0010] This mechanical cutting and centrifugation removes most of the lipids and other useless components (such as swelling fluid) in the fat. This preparation method is simple and easy, and does not require the use of any chemicals or animal-derived preparations, thereby obtaining SVF through this mechanical force treatment.

[0011] However, in actual applications, the cutting filter is often blocked due to problems such as excessive fascia in fat, large fat particles, or small filter aperture, irregular hole shape and low porosity, which makes the cutting process time-consuming and laborious, with low cutting efficiency and poor effect, and even leads to processing failure; or because the pore shape is difficult to process, the pore shape is not ideal, which affects the use, and the defective rate is high, and the processing cost is high. Although the large-aperture filter is labor-saving to operate, it also sacrifices the quality of fat cutting and crushing.

[0012] On the other hand, affected by the cutting resistance, the target fat particle size is difficult to obtain in one cutting, so it needs to be cut through filters of different apertures once or multiple times. The operation is complicated and difficult. In addition, due to the large cavity volume of the cutter, more fat will remain in the cutter, resulting in a low fat yield after cutting.

[0013] Currently, the cutting nets on the market mainly adopt an irregular mesh design, the purpose of which is to improve the cutting performance. However, the irregular shape design of the cutting net will lead to greater processing difficulty (chemical etching, laser processing, etc.), high difficulty in processing quality control (the shape of the processed holes is unstable), unstable filter quality, affecting the cutting efficiency, and the cutting device is complex to process and has high processing costs. Summary of the invention

[0014] The present application provides a fat cutting filter with a simple structure and high fat cutting utilization rate.

[0015] The present application discloses a fat cutting filter, comprising:

[0016] A shell, the interior of the shell is a processing chamber, and the shell has a first interface and a second interface communicating with the processing chamber;

[0017] A cutting piece is located in the processing chamber and between the first interface and the second interface. The cutting piece includes a rigid wire mesh for cutting fat. The wire diameter of the rigid wire mesh is 0.05 mm to 1 mm.

[0018] Several optional methods are also provided below, but they are not intended to be additional limitations on the above-mentioned overall solution, but are merely further supplements or preferences. Under the premise that there are no technical or logical contradictions, each optional method can be combined with the above-mentioned overall solution separately, and multiple optional methods can also be combined.

[0019] In one embodiment, the cutting member further comprises a supporting member, and the rigid wire mesh is tautly fixed to the supporting member.

[0020] In one embodiment, the porosity of the rigid mesh is greater than or equal to 80%. The porosity = (s1 / s) x 100%, where s1 is the opening area of ​​the rigid mesh, and s is the total area of ​​the rigid mesh.

[0021] In one embodiment, the porosity of the rigid mesh is greater than or equal to 90%.

[0022] In one embodiment, the diameter of the rigid wire mesh is 0.05 mm to 0.5 mm.

[0023] In one embodiment, the ratio of the hole diameter to the wire diameter of the rigid wire mesh is (8-1):1.

[0024] In one embodiment, the wire diameter of the rigid wire mesh is 0.15 mm to 0.50 mm, and the ratio of the hole diameter to the wire diameter of the rigid wire mesh is (5 to 1):1.

[0025] In one embodiment, the rigid wire mesh is a woven mesh, and the woven mesh is woven in a plain weave or a twisted weave.

[0026] In one embodiment, the pore size of the rigid wire mesh is 300 μm to 3000 μm.

[0027] In one embodiment, the pore size of the rigid mesh is 300 μm to 2500 μm.

[0028] In one embodiment, the rigid wire mesh has a wire diameter of 0.15 mm to 0.30 mm and a pore size of 500 μm to 1500 μm.

[0029] In one embodiment, the mesh shape of the rigid wire mesh is polygonal.

[0030] In one embodiment, the polygon is a triangle, a quadrilateral or a hexagon.

[0031] In one embodiment, the rigid wire mesh is formed by weaving metal wires, and the cross-sectional shape of the metal wires is circular, elliptical or rectangular.

[0032] In one embodiment, the cutting element includes a layer of rigid wire mesh or multiple layers of rigid wire mesh arranged at intervals, and the apertures of each layer of the multiple layers of rigid wire mesh are arranged in sequence.

[0033] In one embodiment, the cutting element comprises multiple layers of rigid wire mesh arranged at intervals along a first direction, and two adjacent layers of the multiple layers of rigid wire mesh are configured in at least one of the following ways:

[0034] a) Different mesh shapes;

[0035] b) Different apertures;

[0036] c) Different wire diameters;

[0037] d) From a viewing angle along the first direction, the mesh wires have different extending directions.

[0038] In one embodiment, one of two adjacent layers of mesh has an aperture D1, and the other has an aperture D2. The distance between the two adjacent layers of mesh is L, and L is 0.5 to 3 times the sum of D1 and D2.

[0039] In one embodiment, L is 0.6 to 2.5 times the sum of D1 and D2.

[0040] In one embodiment, L is 0.8 to 1.5 times the sum of D1 and D2.

[0041] The present application also provides a fat cutting filter, comprising:

[0042] A shell, the interior of the shell is a processing chamber, and the shell has a first interface and a second interface communicating with the processing chamber;

[0043] A cutting piece is located in the processing chamber and between the first interface and the second interface. At least a portion of the cutting piece is a working area for cutting and filtering fat. The porosity of the working area is greater than or equal to 80%.

[0044] In one embodiment, the cutting element comprises:

[0045] A support ring, which is configured separately or is a part of the shell;

[0046] A rigid wire mesh is fixed in the supporting ring and provides the working area. The wire diameter of the rigid wire mesh is 0.15 mm to 0.30 mm, and the pore size is 500 μm to 1500 μm.

[0047] In one embodiment, there is one working area.

[0048] In one embodiment, the working areas are multiple and arranged at intervals, and between adjacent working areas is a reinforcement portion fixed to the support ring.

[0049] The present application also provides a method for processing a fat cutting filter, comprising:

[0050] Providing metal wires, and pre-weaving the metal wires to obtain a metal wire mesh;

[0051] Providing a support ring, cutting the metal wire mesh into a predetermined shape, and tightening and fixing the cut metal wire mesh to the support ring;

[0052] A shell is provided, wherein the shell has a first interface and a second interface, the cutting piece is encapsulated in the shell, and the cutting piece is located between the first interface and the second interface.

[0053] The present application also provides a fat cutting and filtering method, comprising:

[0054] squeezing the fat tissue to be processed through a predetermined channel;

[0055] Pre-arranging a rigid wire mesh in the predetermined channel, and cutting the fat tissue through the wires of the rigid wire mesh, wherein the wire diameter of the rigid wire mesh is 0.05 mm to 1 mm;

[0056] Along the flow direction of the fat tissue, the rigid wire mesh is a single layer or multiple layers arranged at intervals, and two adjacent layers of the multi-layer rigid wire mesh adopt at least one of the following configurations:

[0057] a) Different mesh shapes;

[0058] b) Different apertures;

[0059] c) Different wire diameters;

[0060] d) From a viewing angle along the first direction, the mesh wires have different extending directions.

[0061] In one embodiment, the fat tissue passes through the rigid mesh in a single flow direction or in a bidirectional reciprocating manner.

[0062] The present application also provides a fat cutting and filtering device, comprising:

[0063] A fat cutting filter, using the fat cutting filter described in the present application;

[0064] A driving device is connected to the first interface and the second interface, and drives the fat tissue to be processed to pass through the cutting member.

[0065] Compared with the prior art, this application has the following beneficial effects:

[0066] 1) The fat cutting filter of the present application has a cutting filter mesh that is a rigid wire mesh woven with ultra-fine wires. The aperture of the cutting filter mesh can be customized as needed, and its porosity can reach more than 90%. The mesh of the cutting filter mesh is more regular, the aperture is more uniform, and the cutting efficiency is higher, which can effectively solve the problem of fat cutting filter blockage. At the same time, the weaving process is simpler than the traditional chemical etching and laser processing process, and has more processing cost advantages. In addition, the fat cutting filter with a combination of multiple layers of rigid wire mesh can make fat cutting easier and more efficient, and improve the utilization rate of fat cutting and the total amount of cutting products.

[0067] 2) The SVF product cut by the fat cutting filter of the present application has better physical properties and biological indicators: lower free oil and free water content; higher total adipose tissue cell amount and cell survival rate, higher adipose stem cell content, and better potential for promoting tissue repair and regeneration. BRIEF DESCRIPTION OF THE DRAWINGS

[0068] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the conventional technology, the drawings required for use in the embodiments or the conventional technology descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0069] Figure 1 This is a schematic diagram of the structure of a fat cutting filter in one embodiment of the present application;

[0070] Figure 2 for Figure 1 A schematic diagram of the structure of the medium fat cutting filter from another angle;

[0071] Figure 3 This is a schematic diagram of the structure of a fat cutting filter (single-layer cutting element) in one embodiment of the present application;

[0072] Figure 4 for Figure 3 Enlarged view of part A;

[0073] Figure 5 This is a schematic structural diagram of a fat cutting filter (multi-layer cutting element) in another embodiment of the present application;

[0074] Figure 6 for Figure 5 Exploded view of the middle multi-layer cutting part;

[0075] Figure 7 for Figure 5 Cross-sectional view of the medium fat cutting filter;

[0076] Figure 8 is a schematic diagram of the arrangement of multiple layers of metal wire mesh;

[0077] Fig. 9 A schematic diagram of multiple layers of metal wire mesh spaced apart from each other;

[0078] Fig.10 This is a schematic diagram of the principle of a fat cutting and filtering device in one embodiment of the present application;

[0079] Fig.11 This is a schematic diagram of the principle of a fat cutting and filtering device in another embodiment of the present application;

[0080] Fig.12 Comparison chart of fat content after cutting in Experimental Example 1 of this application;

[0081] Fig.13 Comparison chart of free oil and free water after cutting in Experimental Example 2 of this application;

[0082] Fig.14 Comparison chart of SVF volume after cutting in Experimental Example 3 of this application.

[0083] The reference numerals of the components are as follows:

[0084] 100, housing; 110, first half housing; 111, first interface; 120, second half housing; 121, second interface; 122, positioning member;

[0085] 200, cutting piece; 200a, first cutting piece; 200b, second cutting piece; 200c, third cutting piece; 210, wire mesh; 211, wire; 212, mesh; 220, support ring;

[0086] 300, cage;

[0087] 400, driving device; 410, pipeline; 420, first pump body; 430, second pump body. DETAILED DESCRIPTION

[0088] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0089] It should be noted that when a component is referred to as being "fixed to" or "disposed on" another component, it may be directly on the other component or there may also be a central component. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may be a central component at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in the specification of this application are for illustrative purposes only and do not represent the only implementation method.

[0090] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0091] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first feature is directly in contact with the second feature, or the first feature and the second feature are indirectly in contact through an intermediate medium. Moreover, a first feature being “above”, “above”, or “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level (or in a state of use, or from a certain perspective of the drawing) than the second feature. A first feature being “below”, “below”, or “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level (or in a state of use, or from a certain perspective of the drawing) than the second feature.

[0092] Unless otherwise defined, all technical and scientific terms used in the specification of this application have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used in the specification of this application includes any and all combinations of one or more related listed items.

[0093] See also Figure 1 to Figure 7 In one embodiment of the present application, a fat cutting filter is provided, including a housing 100 and a cutting member 200. In order to facilitate processing and assembly, the housing 100 can be fixed by splitting and snapping, for example, the housing 100 includes a first half shell 110 and a second half shell 120, which can be fixed by ultrasonic welding to form a processing chamber inside the housing 100 for cutting and filtering fat tissue.

[0094] In order to communicate with external pipelines or equipment, the housing 100 has a first interface 111 and a second interface 121 that are communicated with the processing chamber, and each interface is respectively arranged on a corresponding half shell.

[0095] The shell 100 has a middle part and two opposite ends. The middle part extends with equal diameter, and the shapes of the two ends gradually converge until a tubular interface is formed, namely a first interface 111 and a second interface 121 respectively. The specific structure can adopt a Luer connector or the like to facilitate quick assembly.

[0096] The cutting member 200 is located in the processing chamber, roughly in the middle of the housing 100. The cutting member 200 is located between the first interface 111 and the second interface 121, that is, the fat tissue entering and exiting through each interface flows through the cutting member 200 to be cut and filtered. As one of the key points of improvement of the present application, the cutting member 200 adopts a rigid wire mesh, where "rigidity" does not strictly limit the elastic modulus of the material. As a mesh structure, it at least ensures that the mesh surface is not easy to produce concave and convex deformation during use, and the mesh shape and size remain stable to ensure the cutting and filtering effect. The following embodiments take the metal wire mesh 210 as an example. In order to ensure "rigidity" and cutting effect, the metal wire can be made of stainless steel and other materials. After being tightened, the mesh structure as a whole has a higher strength and is not easy to deform under normal use conditions. The metal wire mesh 210 is woven by metal wires 211. From the perspective of the local action position, the metal wire 211 performs wire cutting on the fat tissue, that is, the wire is used as a blade. As for the cutting member 200 as a whole, a larger porosity can be obtained, and the fat is not easy to be blocked during cutting and filtering, and the operation is labor-saving.

[0097] In one embodiment, in order to maintain the shape and spatial posture of the metal wire 211, the cutting member 200 further includes a support member, and the metal wire 211 is tightened and fixed to the support member. The support member can be, for example, a support ring 220, of course, the support ring 220 is not strictly limited to a circular ring or a circumferentially closed ring, and the metal wire 211 is tightened and fixed in the support ring 220. This embodiment takes a circumferentially closed circular ring as an example, and can obtain circumferentially uniform mechanical properties and a larger area / circumference ratio.

[0098] The support ring 220 can be a separate component installed in the housing 100, or a part of the housing 100 can be directly used as the support ring 220. For the same cutting piece, at least a part is a working area for cutting and filtering fat, that is, the metal wire 211 is exposed to the working area. The working area can be a continuous distribution, that is, the working area is all metal wires 211, or the working area is arranged at intervals. Multiple, that is, the metal wires are distributed in multiple areas, and between adjacent working areas is a reinforcement part fixed to the support ring 220, which can not only ensure the structural strength, but also design different working areas to be in different flow channels, that is, the fat tissue passes through different working areas at different stages. The outer periphery of the support ring 220 is sealed with the inner wall of the housing 100 to ensure that all fat tissue flows through the working area.

[0099] As for the weaving principle of the metal mesh 210, it can be combined with the existing technology. In some embodiments, the weaving method adopted is plain weaving or twisted weaving. Plain weaving is a weaving method in which the warp and weft are interlaced. After being tightened, the warp and weft overlap each other to limit the position of the intersection of the warp and weft, and maintain a stable mesh shape and size. Twisted weaving also includes common warp weaving, in which adjacent warp threads are woven around each other to form a grid structure. The metal mesh 210 can obtain a larger porosity and a more uniform pore size, so that the size of the fat tissue after cutting is more uniform. The weaving method is relatively simple to process, with low cost, and can ensure the cutting effect, so as to implement efficient fat cutting.

[0100] Due to the use of wire cutting, the overall porosity of the metal mesh 210 can be much higher than that of the cut filter component in the prior art. In the present application, the porosity of the metal mesh 210 can be greater than or equal to 80%, and further can be greater than or equal to 90%.

[0101] In one embodiment, in order to take into account both the structural strength of the metal mesh 210 and the cutting and filtering effect on fat tissue, the wire diameter of the metal mesh 210, that is, the diameter of the metal wire 211, is 0.05 mm to 1 mm, and more preferably 0.05 mm to 0.5 mm.

[0102] Related to this is the aperture of the metal mesh 210 , that is, the size of the mesh 212 . In one embodiment, the size of the mesh 212 is 300 μm to 3000 μm, and more preferably 300 μm to 2500 μm.

[0103] The cross-sectional shape of the metal wire 211 can be circular, elliptical, or rectangular. When the cross-sectional shape is non-circular, the wire diameter mentioned above can be understood as the diameter of a circle of equal area.

[0104] The mesh 212 is in a polygonal shape, such as a triangle, a quadrilateral (such as a parallelogram, or a rectangle or a rhombus), a hexagon, etc. The aperture of the mesh 212 mentioned above can be understood as the side length of a square of equal area.

[0105] In some of the embodiments below, the diameter of the metal wire 211 and the aperture of the mesh 212 are further optimized to take into account both cutting and filtering effects and reduce clogging. For example, the ratio of the aperture of the metal wire mesh 210 to the wire diameter is (8-1):1. As a further preferred embodiment, the wire diameter of the metal wire mesh 210 is 0.15mm-0.50mm and the ratio of the aperture of the metal wire mesh 210 to the wire diameter is (5-1):1.

[0106] For another example, the diameter of the metal wire 211 is 0.15 mm to 0.30 mm, and the aperture of the mesh 212 is 500 μm to 1500 μm. The physical properties and cell biological properties of the product SVF are optimal.

[0107] See also Figure 3 In one embodiment, the cutting element 200 is a layer, which can be clamped and positioned in the housing 100 by the positioning element 122 in the housing 100. The positioning element 122 can be a plurality of positioning columns, etc. If necessary, a positioning hole for the positioning element 122 to pass through can be opened on the support ring 220 to facilitate mutual cooperation.

[0108] See also Figure 5 to Figure 7 In one embodiment, the cutting element 200 is a multi-layered metal mesh arranged at intervals, which can further improve the fat utilization rate, increase the product volume, reduce the free oil and free water content, and achieve better target size performance after cutting. The multi-layer cutting element 200 can maintain a relative position relationship through the retaining frame 300 and the positioning element 122 in the housing 100, and can also be directly processed into a pre-assembled body, that is, the multi-layer cutting elements are pre-fixed to each other through their respective support rings 220, and then the whole is encapsulated in the housing 100.

[0109] When a multi-layer (e.g., 2 to 6 layers) structure is used, the apertures of each metal mesh 210 are arranged in sequence, which can make the product's biological index performance more excellent, the simulated clinical performance better, the fat stem cell content higher, and the total cell count and cell survival rate higher. For example, the three layers of metal mesh arranged at intervals can be seen in the figure, namely the first cutting piece 200a, the second cutting piece 200b, and the third cutting piece 200c, the apertures of the three are successively smaller, and the wire diameters can also be successively smaller. When in use, the fat tissue can be cut and filtered in sequence through the meshes from large to small to obtain the desired cutting product.

[0110] When using multiple layers of metal wire mesh, in order to improve the diversion and cutting effects, the mesh shapes of two adjacent metal wire meshes can be different, for example, one is square and the other is hexagonal, and on this basis, different apertures and / or wire diameters can be further combined.

[0111] See also Figure 8 Taking two adjacent metal wire meshes as an example, the aperture and wire diameter of the first cutting piece 200a are both larger than those of the second cutting piece 200b. Moreover, the metal wires of the two adjacent metal wire meshes may have different extension trends. For example, the extension trends of the metal wires of the first cutting piece 200a and the second cutting piece 200b are approximately at an angle of 45°. From the perspective of the figure, the metal wires of the two are mutually obliquely crossed to form a complex spatial network.

[0112] Taking the vertical interweaving of warp and weft of each metal wire mesh as an example, the extension trend of the warp (or weft) of two adjacent metal wire meshes has an angle of 30° to 60°. In a system of multiple metal wire meshes, the mesh holes are staggered, which can further change the fluidity of the fat tissue to be cut, and can cut the fat more efficiently.

[0113] Research has shown that, combined with the application scenario of this application, the distance between two adjacent layers of mesh has a certain influence on the cutting effect and cutting filtration resistance. Fig. 9 , taking the first cutting piece 200a and the second cutting piece 200b as an example, one has an aperture D1, and the other has an aperture D2, and the distance between the two is L, and L is 0.5 to 6 times the sum of D1 and D2. In the figure, L is about 1 to 1.5 times (D1 + D2). The appropriate distance can not only obtain better fluid distribution, but also reduce blockage.

[0114] Based on the above embodiments, an embodiment of the present application further provides a method for processing a fat cutting filter, comprising:

[0115] Providing metal wires, and pre-weaving the metal wires to obtain a metal wire mesh;

[0116] Providing a support ring, cutting the metal wire mesh into a predetermined shape, and tightening and fixing the cut metal wire mesh on the support ring. For example, the support rings can be distributed in pairs, and the same pair of support rings clamp and fix the edge of the metal wire mesh;

[0117] A shell is provided, wherein the shell has a first interface and a second interface, and a cutting piece is encapsulated in the shell, and the cutting piece is located between the first interface and the second interface.

[0118] See also Fig.10 In one embodiment of the present application, a fat cutting filter device is also provided, including the fat cutting filter of the above embodiments and a driving device 400. The driving device 400 is connected to the first interface and the second interface on the shell 100 through a pipeline 410 to drive the fat tissue to be processed through the cutting piece.

[0119] The driving device 400 can be a manual pump or an electric pump. In this embodiment, the driving device 400 is a set that drives the fat tissue to circulate along a single flow direction through the fat cutting filter. Of course, fluid reversal can be performed depending on the type of the driving device 400.

[0120] See also Fig.11 In one embodiment of the present application, a fat cutting and filtering device is provided. The difference from the above embodiment is that the driving device 400 is composed of two sets, namely the first pump body 420 and the second pump body 430. For example, a manual syringe is used. The two sets of driving devices 400 push alternately to make the fat tissue reciprocate through the fat cutting filter in both directions. After pushing back and forth multiple times (odd number of times), the fat can be cut into another syringe.

[0121] Based on the above embodiments, an embodiment of the present application further provides a fat cutting and filtering method, comprising:

[0122] Squeeze the fat tissue to be processed through a predetermined channel, where the predetermined channel can be understood as the interior of the housing and connected between the first interface and the second interface;

[0123] A metal wire is pre-arranged in a predetermined channel, and the fat tissue is cut by the metal wire. The metal wire can be arranged in the predetermined channel in a variety of combinations, such as the metal wire mesh used in the above embodiments;

[0124] Along the flow direction of the fat tissue, the metal wire includes one or more units. In the same unit (i.e. corresponding to a layer of rigid wire mesh), the metal wires are staggered and form mesh holes that allow fat tissue to pass through. The wire diameter and hole diameter of the metal wire can be combined with the previous embodiments.

[0125] After passing through the fat cutting filter, the fat tissue is cut into uniform fat particles, and the free oil can be controlled within 10%. According to a single cutting filter with different pore sizes or a combination thereof, the free oil and free water can be controlled at different levels to meet different clinical demand scenarios (the treatment of osteoarthritis needs to be controlled below 5%, and medical and aesthetic filling can be appropriately relaxed to below 10%).

[0126] The total number of cells in the adipose tissue prepared by the fat cutting filter of the present application can reach 1×10 6 cells / mL, the cell survival rate can reach over 60%, and the adipose stem cell content can reach over 40%.

[0127] In addition, free oil, free water, total cell amount, cell survival rate, and adipose stem cell content can be regulated by the arrangement and combination of cut filters with different pore sizes, such as the combination of 1500μm and 1000μm or the combination of 1500μm, 1000μm and 500μm.

[0128] The following further provides a number of embodiments and test examples to illustrate the use of the fat cutting filter of the present application and the corresponding effects.

[0129] Example 1

[0130] The fat tissue is passed through a fat cutting filter with only one cutting element (such as Figure 3 As shown) for cutting, use Fig. 9 In the manner shown, the fat tissue is repeatedly pushed through a syringe for cutting and filtering.

[0131] Example 2

[0132] The fat tissue is cut through multiple fat cutting filters in sequence. For a single fat cutting filter, only one cutting element (such as Figure 3 As shown), but the wire mesh apertures in the plurality of fat cutting filters are arranged in sequence from large to small.

[0133] Example 3

[0134] The fat tissue is passed through a fat cutting filter with multiple cutting elements (such as Figure 5 The mesh is cut as shown in the figure, the mesh apertures are arranged from large to small, and the fat tissue is repeatedly pushed through a syringe for cutting and filtering.

[0135] Example 4

[0136] The fat cutting filter of Example 1 was cut and compared by changing the wire diameter and pore size of the metal wire mesh, and the free oil and free water contents in the cut tissue were tested respectively. The test results are as follows: Fig.10 , Fig.11 shown.

[0137] Test Example 1: Testing the fat particle size of different samples

[0138] The fat particles were dispersed in phosphate buffered saline (PBS) and then placed in a sample tank for particle size analysis. The particle size distribution results were obtained by laser scattering particle size distribution instrument. Fig.12 .

[0139] In the figure:

[0140] Example 1: The fat cutting filter described in Example 1 is used to cut fat, wherein the wire diameter of the metal mesh is 0.3-0.5 mm and the pore size is 1500 μm;

[0141] Example 2: The fat is cut using the plurality of fat cutting filters described in Example 2 above, wherein the pore sizes of the metal meshes of the fat cutting filters are 1500 μm, 1000 μm and 500 μm, respectively, and the wire diameters are 0.3-0.5 mm, 0.2-0.3 mm and 0.05-0.2 mm, respectively;

[0142] Example 3: The fat cutting filter described in Example 3 is used to cut fat, the pore sizes of the metal mesh layers are 1500 μm, 1000 μm and 500 μm, respectively, and the wire diameters are 0.3-0.5 mm, 0.2-0.3 mm and 0.05-0.2 mm, respectively;

[0143] Non-mesh: Use commercially available non-mesh cut filters (pore size 500-600 μm).

[0144] Test Example 2: Testing the free oil, free water content and SVF yield in different samples

[0145] Take the fat cutting filters of Examples 1 to 3 above and cut the fat respectively. The test results of free oil and free water content in each sample after cutting are shown in Fig.13 , SVF yield test results are shown in Fig.14 ,in:

[0146] The cut filter screen (i.e., metal wire screen) of Example 1 has a pore size of 500 μm;

[0147] Example 2 uses three types of single-layer fat cutting filters, and the pore sizes of the cutting filters are 1500 μm, 1000 μm, and 500 μm respectively;

[0148] Example 3 uses the same fat cutting filter to configure three layers of cutting filter screens, wherein the pore sizes of the three layers of cutting filter screens are 1500 μm, 1000 μm, and 500 μm, respectively.

[0149] The above wire diameters are all controlled at 0.15mm.

[0150] Experimental Example 3: Total cell number per unit volume of SVF product, cell survival rate, and adipose stem cells

[0151] Trypan blue staining: Living cells will not be stained blue by trypan blue, while dead cells will be stained light blue. After trypan blue staining, the cell survival rate can be more accurately quantified by counting directly under a microscope or by taking a photo under a microscope.

[0152] Taking the fat cutting filter with only one cutting piece as an example, the total number of cells per unit volume and the cell survival rate in the SVF product obtained after cutting were tested. The results are shown in Table 1.

[0153] Table 1

[0154]

[0155] Table 2 shows the cell biology test results of the SVF product prepared using the fat cutting filter described in the above embodiments (wherein the wire diameter of the metal mesh is 0.15 mm).

[0156] Table 2

[0157]

[0158] The technical features of the above-mentioned embodiments can be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification. When the technical features in different embodiments are embodied in the same figure, it can be regarded that the figure also discloses the combination examples of the various embodiments involved.

[0159] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the scope of patent protection of the present application shall be subject to the attached claims.

Claims

1. A fat cutting filter, characterized in that: include: A shell, the interior of the shell is a processing chamber, and the shell has a first interface and a second interface communicating with the processing chamber; A cutting piece is located in the processing chamber and between the first interface and the second interface; the cutting piece comprises a rigid wire mesh for cutting fat, and the wire diameter of the rigid wire mesh is 0.05 mm to 1 mm.

2. The fat cutting filter according to claim 1, characterized in that: The ratio of the aperture diameter to the wire diameter of the rigid wire mesh is (8-1):

1.

3. The fat cutting filter according to claim 1, characterized in that: The wire diameter of the rigid wire mesh is 0.15 mm to 0.50 mm, and the ratio of the hole diameter to the wire diameter of the rigid wire mesh is (5 to 1):

1.

4. The fat cutting filter according to claim 1, characterized in that: The rigid wire mesh is a woven mesh, the woven mesh is woven in a flat weave or a twisted weave, and the mesh shape of the rigid wire mesh is a polygon.

5. The fat cutting filter according to any one of claims 1 to 4, characterized in that: The cutting piece includes a layer of rigid wire mesh or multiple layers of rigid wire mesh arranged at intervals, and the apertures of each layer of the multiple layers of rigid wire mesh are arranged in sequence.

6. The fat cutting filter according to claim 1, characterized in that: The cutting element comprises multiple layers of rigid wire mesh arranged at intervals along a first direction, wherein two adjacent layers of the multiple layers of rigid wire mesh adopt at least one of the following configurations: a) Different mesh shapes; b) Different apertures; c) Different wire diameters; d) From a viewing angle along the first direction, the mesh wires have different extending directions.

7. A fat cutting filter, characterized in that: include: A shell, the interior of the shell is a processing chamber, and the shell has a first interface and a second interface communicating with the processing chamber; The cutting piece is located in the processing chamber and between the first interface and the second interface. At least a part of the cutting piece is a working area for cutting and filtering fat. The porosity of the working area is greater than or equal to 80%.

8. The fat cutting filter according to claim 7, characterized in that: The cutting element comprises: A support ring, which is configured separately or is a part of the shell; A rigid wire mesh is fixed in the supporting ring and provides the working area. The wire diameter of the rigid wire mesh is 0.15 mm to 0.30 mm, and the pore size is 500 μm to 1500 μm.

9. A fat cutting and filtering device, characterized in that: include: A fat cutting filter, comprising the fat cutting filter according to any one of claims 1 to 8; A driving device is connected to the first interface and the second interface, and drives the fat tissue to be processed to pass through the cutting member.

10. A fat cutting and filtering method, characterized in that: include: squeezing the fat tissue to be processed through a predetermined channel; Pre-arranging a rigid wire mesh in the predetermined channel, and cutting the fat tissue through the wires of the rigid wire mesh, wherein the wire diameter of the rigid wire mesh is 0.05 mm to 1 mm; Along the flow direction of the fat tissue, the rigid wire mesh is one or multiple rigid wire meshes arranged at intervals, and the adjacent two layers of the rigid wire meshes are arranged in at least one of the following ways: a) Different mesh shapes; b) Different apertures; c) Different wire diameters; d) From a viewing angle along the first direction, the mesh wires have different extending directions.

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