A liquid cleaning agent based on hydrogen peroxide and a preparation method thereof

Abstract

The invention relates to the field of medical device cleaning agents, and in particular to a liquid cleaning agent based on hydrogen peroxide and a preparation method thereof. The liquid cleaning agent comprises an agent A and an agent B; the mass ratio of the agent A to the agent B is 60-70:30-40; the agent A of the invention introduces a double-chain quaternary ammonium salt and PCA zinc, thereby accelerating the contact between hydrogen peroxide molecules and pathogens, and achieving pathogen killing in a relatively short time; 4-formylphenylboric acid in the agent B can effectively inhibit protease activity during storage, prevent protease hydrolysis, and protect the enzyme; the hydrogen peroxide in the compounded agent A can slowly restore the enzyme activity during use, so that the enzyme can play a role to the maximum extent; the added composite stabilizer and glycerol have a stabilizing effect on the protease, and prevent the enzyme activity from being reduced; the composite corrosion inhibitor and the corrosion inhibitor can effectively prevent the medical device from being corroded by the cleaning agent, and therefore has a wide application prospect.

Description

Technical Field

[0001] The invention relates to the field of medical device cleaning agents, and in particular to a hydrogen peroxide-based liquid cleaning agent and a preparation method thereof. Background Art

[0002] Cleaning, disinfection and sterilization of medical devices are the key to preventing and controlling hospital-acquired infections and ensuring medical quality. Good cleaning is the prerequisite for disinfection and sterilization. If they are not adequately cleaned, disinfection and sterilization will also fail. Therefore, the quality of cleaning will directly affect the quality of medical care and the safety of patients. As more complex medical devices are gradually used in hospitals, the difficulty of cleaning during the processing process continues to increase. The use of cleaning agents plays a decisive role in effective cleaning.

[0003] After use, medical devices often have organic pollutants such as blood, oil and tissue fluid remaining on their surfaces. In addition, many medical devices will cause the accumulation of biofilms during repeated use. Biofilms are very harmful, and after the formation of biofilms, 2,400 times the amount of antibiotics is required, with a mortality rate of up to 50%-60%. It is far from enough to rely solely on disinfection / sterilization to kill the bacteria in the biofilm. Only by thoroughly removing the biofilm can the risk of infection be effectively reduced. Without high-quality cleaning, there will be no qualified sterilization. However, the main problem with existing cleaning agents is that the cleaning is not thorough, which causes pollutants to remain on the surface of the device, greatly damaging the device and shortening its service life.

[0004] Existing conventional high-efficiency disinfection cleaning agents often contain chloride ions, which are easy to remain and cause corrosion of medical devices, while ultraviolet disinfection cannot enter the dead corners of medical devices, making it difficult to ensure the effect. Hydrogen peroxide disinfectant is a highly oxidizing and high-efficiency disinfectant with the characteristics of no residue after use, green and pollution-free. Hydrogen peroxide molecules release hydroxyl free radicals and new ecological atoms with strong oxidizing ability in water. Once these active particles come into contact with microorganisms, they will destroy the microbial protein structure, causing the death of microorganisms, and achieving the effect of inactivating microorganisms. However, hydrogen peroxide molecules themselves are not stable enough. Therefore, in order to ensure the content of hydrogen peroxide and reduce decomposition during storage, some stabilizers are usually added. Traditional stabilizers inhibit the decomposition of hydrogen peroxide while also reducing its reaction activity. Therefore, the concentration of hydrogen peroxide disinfectants on the market is generally high (generally 3%), which has certain safety hazards during storage and use. At the same time, high concentrations of hydrogen peroxide cause waste of raw materials, which is not in line with the concept of green chemistry.

[0005] Therefore, according to the above-mentioned related technologies, it is urgent to develop a liquid cleaning agent based on hydrogen peroxide and a preparation method thereof. Summary of the invention

[0006] In view of this, the purpose of the present invention is to provide a liquid cleaning agent based on hydrogen peroxide and a preparation method thereof, so as to solve the problems of weak sterilization ability and poor storage stability of disinfection cleaning agents for medical devices in the prior art.

[0007] Based on the above objectives, the present invention provides a hydrogen peroxide-based liquid cleaning agent and a preparation method thereof.

[0008] A hydrogen peroxide-based liquid cleaning agent, the liquid cleaning agent comprising agent A and agent B;

[0009] The mass ratio of the A agent to the B agent is 60-70:30-40;

[0010] The agent A is prepared from the following raw materials: hydrogen peroxide, double-chain quaternary ammonium salt, PCA zinc, composite stabilizer, composite corrosion inhibitor and pH regulator;

[0011] The agent B is prepared from the following raw materials: triethanolamine oleic acid soap, benzotriazole, propylene glycol monomethyl ether, glycerol, additives, surfactants, corrosion inhibitors and deionized water;

[0012] The additive is obtained by mixing a multi-enzyme additive and an auxiliary enzyme additive in a mass ratio of 3-4:1-2;

[0013] The multi-enzyme additive is prepared from the following raw materials: calcium chloride, 4-formylphenylboric acid, propylene glycol monomethyl ether, glycerol and composite enzyme.

[0014] Preferably, the preparation process of agent A is as follows:

[0015] Step A1. Add hydrogen peroxide to deionized water and stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A1;

[0016] Step A2. Add the composite stabilizer to the mixed solution A1, and stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A2;

[0017] Step A3. Add the double-chain quaternary ammonium salt to the mixed solution A2, and stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A3;

[0018] Step A4. Add PCA zinc to the mixed solution A3, and stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A4;

[0019] Step A5. Add the composite corrosion inhibitor to the mixed solution A4, stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A5;

[0020] Step A6. Add the pH adjuster to the mixed solution A5, stir at a speed of 60-90 r / min for 14-16 min to obtain Agent A.

[0021] Preferably, the mass ratio of hydrogen peroxide to deionized water in step A1 is 0.875-1.15:80-85;

[0022] The mass ratio of the composite stabilizer to the mixed solution A1 in step A2 is 0.05-0.15:80-85;

[0023] The composite stabilizer in step A2 is obtained by mixing sodium pyrophosphate and hydroxyethylidene diphosphonic acid in a mass ratio of 1.8-2.2:0.8-1.2.

[0024] Preferably, the mass ratio of the double-chain quaternary ammonium salt and the mixed solution A2 in step A3 is 0.18-0.22:80-85;

[0025] The double-chain quaternary ammonium salt in step A3 is any one of didecyldimethylammonium chloride, didecyldimethylammonium bromide, octyldecyldimethylammonium chloride, octyldecyldimethylammonium bromide, dioctyldimethylammonium chloride and dioctyldimethylammonium bromide;

[0026] The mass ratio of PCA zinc to mixed solution A3 in step A4 is 0.6-1.5:80-85.

[0027] Preferably, the mass ratio of the composite corrosion inhibitor to the mixed solution A4 in step A5 is 0.08-0.2:80-85;

[0028] The composite corrosion inhibitor in step A5 is obtained by mixing thiourea and benzotriazole in a mass ratio of 0.8-1.2:1.8-2.2;

[0029] The mass ratio of the pH adjuster to the mixed solution A5 in step A6 is 0.1-0.2:80-85;

[0030] The pH adjuster in step A6 is any one of phosphoric acid and sodium phosphate.

[0031] Preferably, the preparation process of agent B is as follows:

[0032] Step B1. Evenly mixing the multi-enzyme additive and the auxiliary enzyme additive to obtain an additive;

[0033] Step B2. Disperse triethanolamine oleate soap, benzotriazole, propylene glycol monomethyl ether, glycerol and additives in deionized water and mix well to obtain a mixed solution B1;

[0034] Step B3. Add a surfactant and a corrosion inhibitor to the mixed solution B1, mix well, and obtain agent B.

[0035] Preferably, the preparation process of the multi-enzyme additive in step B1 is as follows: calcium chloride, 4-formylphenylboric acid, propylene glycol monomethyl ether, glycerol and composite enzyme are mixed, and stirred at 25-30° C. for 30-35 minutes to obtain the multi-enzyme additive;

[0036] The mass ratio of calcium chloride, 4-formylphenylboric acid, propylene glycol monomethyl ether, glycerol and complex enzyme in step B1 is 4-5: 0.6-0.8: 2-3: 6-8: 3-5;

[0037] The complex enzyme is obtained by mixing protease, lipase, xylitolase, cellulase and amylase in a mass ratio of 10-12:4-6:1-3:0.8-1.2:0.8-1.2;

[0038] The auxiliary enzyme additive in step B1 is obtained by mixing lysozyme, amylase and lipase in a mass ratio of 6-9:2-5:2-6.

[0039] Preferably, the mass ratio of triethanolamine oleate soap, benzotriazole, propylene glycol monomethyl ether, glycerol, additives and deionized water in step B2 is 8-12:0.4-0.6:1-2:1-1.5:3-5:54.9-67.6;

[0040] The mass ratio of the mixed solution B1, the surfactant and the corrosion inhibitor in step B3 is 66.9-86.6:16-20:2.5-4.

[0041] Preferably, the surfactant in step B3 is obtained by mixing triethanolamine oleic acid soap and AEO-9 in a mass ratio of 8-12:38-42;

[0042] The corrosion inhibitor in step B3 is sodium gluconate.

[0043] A method for preparing a liquid cleaning agent based on hydrogen peroxide comprises the following steps:

[0044] Mix Agent A and Agent B evenly to obtain a hydrogen peroxide-based liquid cleaning agent.

[0045] Beneficial effects of the present invention:

[0046] The invention provides a liquid cleaning agent based on hydrogen peroxide and a preparation method thereof. In the agent A of the invention, double-chain quaternary ammonium salt and PCA zinc are introduced. During the cleaning process, the double-chain quaternary ammonium salt can neutralize the charges on the surfaces of bacteria and viruses, promote the aggregation of pathogens, and thus accelerate the contact between hydrogen peroxide molecules and pathogens. At the same time, when the double-chain quaternary ammonium salt is adsorbed and consumed, the PCA zinc in the system will be partially hydrolyzed to produce nano zinc oxide, which can activate the decomposition of hydrogen peroxide to produce hydroxyl free radicals with stronger oxidizing ability, thereby achieving the killing of pathogens in a relatively short time.

[0047] The invention provides a liquid cleaning agent based on hydrogen peroxide and a preparation method thereof. The 4-formylphenylboric acid introduced in the agent B of the invention can effectively inhibit the activity of a protease during storage, prevent the protease from being hydrolyzed by itself or being hydrolyzed by other enzymes, and protect the enzyme. The hydrogen peroxide in the compound agent A can slowly restore the activity of the enzyme during use, so that the enzyme can function to the maximum extent. The propylene glycol anisole therein can assist the dissolution of additives. The added composite stabilizer and glycerol have a smaller hydrogen bond capacity than water, and have less destructive power on the hydrogen bond structure of protein molecules. The liquid cleaning agent can replace water as a solvent to have a stabilizing effect on the protease, form a coordinated effect to reduce damage to the enzyme, and prevent the enzyme activity from being reduced. The introduced composite corrosion inhibitor and corrosion inhibitor can effectively prevent the medical device from being corroded by the cleaning agent while cleaning, disinfecting and sterilizing the medical device, and therefore has a wide application prospect. DETAILED DESCRIPTION

[0048] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments.

[0049] Embodiment 1: A method for preparing a liquid cleaning agent based on hydrogen peroxide, comprising the following steps:

[0050] S1. 0.875 g of hydrogen peroxide was added to 80 g of deionized water and stirred at 60 r / min for 5 min to obtain a mixed solution A1;

[0051] S2. 1.8 g of sodium pyrophosphate and 0.8 g of hydroxyethylidene diphosphonic acid were mixed to obtain a composite stabilizer;

[0052] S3. 0.05 g of the composite stabilizer was added to 80 g of the mixed solution A1 and stirred at a speed of 60 r / min for 5 min to obtain a mixed solution A2;

[0053] S3. 0.18 g of didecyl dimethyl ammonium chloride was added to 80 g of the mixed solution A2 and stirred at a speed of 60 r / min for 5 min to obtain a mixed solution A3;

[0054] S4. 0.6 g PCA zinc was added to 80 g of the mixed solution A3 and stirred at a speed of 60 r / min for 5 min to obtain a mixed solution A4;

[0055] S5. 0.8 g of thiourea and 1.8 g of benzotriazole were mixed to obtain a composite corrosion inhibitor;

[0056] S6. 0.08 g of the composite corrosion inhibitor was added to 80 g of the mixed solution A4 and stirred at a speed of 60 r / min for 5 min to obtain a mixed solution A5;

[0057] S7. 0.1 g of phosphoric acid was added to 80 g of the mixed solution A5 and stirred at a speed of 60 r / min for 14 min to obtain agent A;

[0058] S8. 10 g of protease, 4 g of lipase, 1 g of xylitolase, 0.8 g of cellulase and 0.8 g of amylase were mixed to obtain a composite enzyme;

[0059] S9. 4 g of calcium chloride, 0.6 g of 4-formylphenylboric acid, 2 g of propylene glycol monomethyl ether, 6 g of glycerol and 3 g of complex enzyme were mixed and stirred at 25 ° C for 30 min to obtain a multi-enzyme additive;

[0060] S10. 6 g of lysozyme, 2 g of amylase and 2 g of lipase were mixed to obtain an auxiliary enzyme additive;

[0061] S11. The 3g multi-enzyme additive and 1g auxiliary enzyme additive were mixed to obtain an additive;

[0062] S12. 8 g of triethanolamine oleate soap, 0.4 g of benzotriazole, 1 g of propylene glycol monomethyl ether, 1 g of glycerol and 3 g of additives were dispersed in 54.9 g of deionized water and mixed to obtain a mixed solution B1;

[0063] S13. 8 g of triethanolamine oleic acid soap and 38 g of AEO-9 were mixed to obtain a surfactant;

[0064] S14. To 66.9 g of the mixed solution B1, 16 g of a surfactant and 2.5 g of sodium gluconate were added and mixed to obtain agent B;

[0065] S15. Mix 60 g of agent A and 30 g of agent B evenly to obtain a hydrogen peroxide-based liquid cleaning agent.

[0066] Embodiment 2: A method for preparing a liquid cleaning agent based on hydrogen peroxide, comprising the following steps:

[0067] S1. 0.9 g of hydrogen peroxide was added to 81 g of deionized water and stirred at 65 r / min for 6 min to obtain a mixed solution A1;

[0068] S2. 1.85 g of sodium pyrophosphate and 0.9 g of hydroxyethylidene diphosphonic acid were mixed to obtain a composite stabilizer;

[0069] S3. 0.07 g of the composite stabilizer was added to 81 g of the mixed solution A1 and stirred at a speed of 65 r / min for 6 min to obtain a mixed solution A2;

[0070] S3. 0.19 g of didecyl dimethyl ammonium bromide was added to 81 g of the mixed solution A2 and stirred at a speed of 65 r / min for 6 min to obtain a mixed solution A3;

[0071] S4. 0.8 g PCA zinc was added to 81 g of the mixed solution A3 and stirred at a speed of 65 r / min for 6 min to obtain a mixed solution A4;

[0072] S5. 0.9 g of thiourea and 1.9 g of benzotriazole were mixed to obtain a composite corrosion inhibitor;

[0073] S6. 0.12 g of the composite corrosion inhibitor was added to 81 g of the mixed solution A4 and stirred at a speed of 65 r / min for 6 min to obtain a mixed solution A5;

[0074] S7. 0.12 g of sodium phosphate was added to 81 g of the mixed solution A5, and stirred at a speed of 65 r / min for 15 min to obtain agent A;

[0075] S8. 11 g of protease, 4.5 g of lipase, 1.5 g of xylitolase, 0.9 g of cellulase and 0.9 g of amylase were mixed to obtain a composite enzyme;

[0076] S9. 4.2 g of calcium chloride, 0.7 g of 4-formylphenylboric acid, 2.2 g of propylene glycol monomethyl ether, 6.5 g of glycerol and 3.5 g of complex enzyme were mixed and stirred at 26 ° C for 31 min to obtain a multi-enzyme additive;

[0077] S10. 7 g of lysozyme, 3 g of amylase and 3 g of lipase were mixed to obtain an auxiliary enzyme additive;

[0078] S11. 3.2g of multi-enzyme additive and 1.2g of auxiliary enzyme additive were mixed to obtain an additive;

[0079] S12. 9 g of triethanolamine oleate soap, 0.44 g of benzotriazole, 1.2 g of propylene glycol monomethyl ether, 1.1 g of glycerol and 3.4 g of additives were dispersed in 56 g of deionized water and mixed to obtain a mixed solution B1;

[0080] S13. 9 g of triethanolamine oleic acid soap and 39 g of AEO-9 were mixed to obtain a surfactant;

[0081] S14. To 72 g of the mixed solution B1, 17 g of a surfactant and 3 g of sodium gluconate were added and mixed to obtain agent B;

[0082] S15. Mix 62 g of agent A and 32 g of agent B evenly to obtain a hydrogen peroxide-based liquid cleaning agent.

[0083] Embodiment 3: A method for preparing a liquid cleaning agent based on hydrogen peroxide, comprising the following steps:

[0084] S1. 1 g of hydrogen peroxide was added to 82 g of deionized water and stirred at 70 r / min for 7 min to obtain a mixed solution A1;

[0085] S2. 1.9 g of sodium pyrophosphate and 1 g of hydroxyethylidene diphosphonic acid were mixed to obtain a composite stabilizer;

[0086] S3. 0.09 g of the composite stabilizer was added to 82 g of the mixed solution A1 and stirred at a speed of 70 r / min for 7 min to obtain a mixed solution A2;

[0087] S3. 0.2 g of octyldecyl dimethyl ammonium chloride was added to 82 g of the mixed solution A2 and stirred at a speed of 70 r / min for 7 min to obtain a mixed solution A3;

[0088] S4. 1 g PCA zinc was added to 82 g of the mixed solution A3 and stirred at a speed of 70 r / min for 7 min to obtain a mixed solution A4;

[0089] S5. Mix 1 g of thiourea and 2 g of benzotriazole to obtain a composite corrosion inhibitor;

[0090] S6. 0.15 g of the composite corrosion inhibitor was added to 82 g of the mixed solution A4 and stirred at a speed of 70 r / min for 7 min to obtain a mixed solution A5;

[0091] S7. 0.14 g of phosphoric acid was added to 82 g of the mixed solution A5, and stirred at a speed of 70 r / min for 16 min to obtain agent A;

[0092] S8. 12g protease, 5g lipase, 2g xylitolase, 1g cellulase and 1g amylase were mixed to obtain a composite enzyme;

[0093] S9. 4.4 g of calcium chloride, 0.8 g of 4-formylphenylboric acid, 2.4 g of propylene glycol monomethyl ether, 6.8 g of glycerol and 3.8 g of complex enzyme were mixed and stirred at 27 ° C for 32 min to obtain a multi-enzyme additive;

[0094] S10. 8 g of lysozyme, 4 g of amylase and 4 g of lipase were mixed to obtain an auxiliary enzyme additive;

[0095] S11. 3.4 g of multi-enzyme additive and 1.4 g of auxiliary enzyme additive were mixed to obtain an additive;

[0096] S12. 10 g of triethanolamine oleic acid soap, 0.48 g of benzotriazole, 1.4 g of propylene glycol monomethyl ether, 1.2 g of glycerol and 3.8 g of additives were dispersed in 60 g of deionized water and mixed to obtain a mixed solution B1;

[0097] S13. 10 g of triethanolamine oleic acid soap and 40 g of AEO-9 were mixed to obtain a surfactant;

[0098] S14. To 78 g of the mixed solution B1, 18 g of a surfactant and 3.2 g of sodium gluconate were added and mixed to obtain agent B;

[0099] S15. Mix 64 g of agent A and 34 g of agent B evenly to obtain a hydrogen peroxide-based liquid cleaning agent.

[0100] Embodiment 4: A method for preparing a liquid cleaning agent based on hydrogen peroxide, comprising the following steps:

[0101] S1. 1.05 g of hydrogen peroxide was added to 83 g of deionized water and stirred at a speed of 80 r / min for 5 min to obtain a mixed solution A1;

[0102] S2. 2 g of sodium pyrophosphate and 1.1 g of hydroxyethylidene diphosphonic acid were mixed to obtain a composite stabilizer;

[0103] S3. 0.11 g of the composite stabilizer was added to 83 g of the mixed solution A1 and stirred at a speed of 80 r / min for 5 min to obtain a mixed solution A2;

[0104] S3. 0.21 g of octyldecyl dimethyl ammonium bromide was added to 80-81-82-83-84-85 g of the mixed solution A2, and stirred at a speed of 80 r / min for 5 min to obtain a mixed solution A3;

[0105] S4. 1.2 g PCA zinc was added to 83 g of the mixed solution A3 and stirred at a speed of 80 r / min for 5 min to obtain a mixed solution A4;

[0106] S5. 1.1 g of thiourea and 2.1 g of benzotriazole were mixed to obtain a composite corrosion inhibitor;

[0107] S6. 0.16 g of the composite corrosion inhibitor was added to 83 g of the mixed solution A4 and stirred at a speed of 80 r / min for 5 min to obtain a mixed solution A5;

[0108] S7. 0.16 g of sodium phosphate was added to 83 g of the mixed solution A5, and stirred at a speed of 80 r / min for 14 min to obtain agent A;

[0109] S8. 10.5 g of protease, 5.5 g of lipase, 2.5 g of xylitolase, 1.1 g of cellulase and 1.1 g of amylase were mixed to obtain a composite enzyme;

[0110] S9. 4.6 g of calcium chloride, 0.65 g of 4-formylphenylboric acid, 2.6 g of propylene glycol monomethyl ether, 7 g of glycerol and 4 g of complex enzyme were mixed and stirred at 28 ° C for 33 min to obtain a multi-enzyme additive;

[0111] S10. 9 g of lysozyme, 5 g of amylase and 5 g of lipase were mixed to obtain an auxiliary enzyme additive;

[0112] S11. 3.6 g of multi-enzyme additive and 1.6 g of auxiliary enzyme additive were mixed to obtain an additive;

[0113] S12. 11 g of triethanolamine oleate soap, 0.52 g of benzotriazole, 1.6 g of propylene glycol monomethyl ether, 1.3 g of glycerol and 4.2 g of additives were dispersed in 62 g of deionized water and mixed to obtain a mixed solution B1;

[0114] S13. 11 g of triethanolamine oleic acid soap and 41 g of AEO-9 were mixed to obtain a surfactant;

[0115] S14. To 82 g of the mixed solution B1, 19 g of a surfactant and 3.4 g of sodium gluconate were added and mixed to obtain agent B;

[0116] S15. Mix 66 g of agent A and 36 g of agent B evenly to obtain a hydrogen peroxide-based liquid cleaning agent.

[0117] Example 5: A method for preparing a liquid cleaning agent based on hydrogen peroxide, comprising the following steps:

[0118] S1. 1.1 g of hydrogen peroxide was added to 84 g of deionized water and stirred at a speed of 85 r / min for 6 min to obtain a mixed solution A1;

[0119] S2. 2.1g of sodium pyrophosphate and 1.2g of hydroxyethylidene diphosphonic acid were mixed to obtain a composite stabilizer;

[0120] S3. 0.13 g of the composite stabilizer was added to 84 g of the mixed solution A1 and stirred at a speed of 85 r / min for 6 min to obtain a mixed solution A2;

[0121] S3. 0.22 g of dioctyl dimethyl ammonium chloride was added to 84 g of the mixed solution A2 and stirred at a speed of 85 r / min for 6 min to obtain a mixed solution A3;

[0122] S4. 1.3 g PCA zinc was added to 84 g of the mixed solution A3 and stirred at a speed of 85 r / min for 6 min to obtain a mixed solution A4;

[0123] S5. 1.2 g of thiourea and 2.2 g of benzotriazole were mixed to obtain a composite corrosion inhibitor;

[0124] S6. 0.18 g of the composite corrosion inhibitor was added to 84 g of the mixed solution A4 and stirred at a speed of 85 r / min for 6 min to obtain a mixed solution A5;

[0125] S7. 0.18 g of phosphoric acid was added to 84 g of the mixed solution A5, and stirred at a speed of 85 r / min for 15 min to obtain agent A;

[0126] S8. 11.5 g of protease, 6 g of lipase, 2.8 g of xylitolase, 1.2 g of cellulase and 1.2 g of amylase were mixed to obtain a composite enzyme;

[0127] S9. 4.8 g of calcium chloride, 0.75 g of 4-formylphenylboric acid, 2.8 g of propylene glycol monomethyl ether, 7.5 g of glycerol and 4.5 g of complex enzyme were mixed and stirred at 29 ° C for 34 min to obtain a multi-enzyme additive;

[0128] S10. 7.5 g of lysozyme, 3.8 g of amylase and 6 g of lipase were mixed to obtain an auxiliary enzyme additive;

[0129] S11. 3.8 g of multi-enzyme additive and 1.8 g of auxiliary enzyme additive were mixed to obtain an additive;

[0130] S12. 12 g of triethanolamine oleate soap, 0.56 g of benzotriazole, 1.8 g of propylene glycol monomethyl ether, 1.4 g of glycerol and 4.6 g of additives were dispersed in 64 g of deionized water and mixed to obtain a mixed solution B1;

[0131] S13. 12 g of triethanolamine oleic acid soap and 42 g of AEO-9 were mixed to obtain a surfactant;

[0132] S14. Add 20 g of surfactant and 3.6 g of sodium gluconate to 85 g of the mixed solution B1 and mix well to obtain agent B;

[0133] S15. Mix 68 g of agent A and 38 g of agent B evenly to obtain a hydrogen peroxide-based liquid cleaning agent.

[0134] Embodiment 6: A method for preparing a liquid cleaning agent based on hydrogen peroxide, comprising the following steps:

[0135] S1. 1.15 g of hydrogen peroxide was added to 85 g of deionized water and stirred at a speed of 90 r / min for 7 min to obtain a mixed solution A1;

[0136] S2. 2.2g of sodium pyrophosphate and 1g of hydroxyethylidene diphosphonic acid were mixed to obtain a composite stabilizer;

[0137] S3. 0.15 g of the composite stabilizer was added to 85 g of the mixed solution A1 and stirred at a speed of 90 r / min for 7 min to obtain a mixed solution A2;

[0138] S3. 0.21 g of dioctyl dimethyl ammonium bromide was added to 85 g of the mixed solution A2 and stirred at a speed of 90 r / min for 7 min to obtain a mixed solution A3;

[0139] S4. 1.5 g PCA zinc was added to 85 g of the mixed solution A3 and stirred at a speed of 90 r / min for 7 min to obtain a mixed solution A4;

[0140] S5. Mix 1 g of thiourea and 2 g of benzotriazole to obtain a composite corrosion inhibitor;

[0141] S6. 0.2 g of the composite corrosion inhibitor was added to 85 g of the mixed solution A4 and stirred at a speed of 90 r / min for 7 min to obtain a mixed solution A5;

[0142] S7. 0.2 g of sodium phosphate was added to 85 g of the mixed solution A5, and stirred at a speed of 90 r / min for 16 min to obtain agent A;

[0143] S8. 11.8 g of protease, 5.8 g of lipase, 3 g of xylitolase, 1 g of cellulase and 1 g of amylase were mixed to obtain a composite enzyme;

[0144] S9. 5 g of calcium chloride, 0.78 g of 4-formylphenylboric acid, 3 g of propylene glycol monomethyl ether, 8 g of glycerol and 5 g of complex enzyme were mixed and stirred at 30 ° C for 35 min to obtain a multi-enzyme additive;

[0145] S10. 8.5 g of lysozyme, 4.5 g of amylase and 4.5 g of lipase were mixed to obtain an auxiliary enzyme additive;

[0146] S11. 4g of multi-enzyme additive and 2g of auxiliary enzyme additive were mixed to obtain an additive;

[0147] S12. 8.8 g of triethanolamine oleate soap, 0.6 g of benzotriazole, 2 g of propylene glycol monomethyl ether, 1.5 g of glycerol and 5 g of additives were dispersed in 67.6 g of deionized water and mixed to obtain a mixed solution B1;

[0148] S13. 10.6 g of triethanolamine oleic acid soap and 40.8 g of AEO-9 were mixed to obtain a surfactant;

[0149] S14. To 86.6 g of the mixed solution B1, 18.8 g of a surfactant and 4 g of sodium gluconate were added and mixed to obtain agent B;

[0150] S15. Evenly mix 70 g of agent A and 40 g of agent B to obtain a hydrogen peroxide-based liquid cleaning agent.

[0151] Comparative Example 1:

[0152] Compared with Example 1, PCA zinc was not added in the preparation process of the hydrogen peroxide-based liquid cleaning agent in this comparative example, and the remaining steps and parameters were the same, which will not be repeated in this comparative example. Finally, a hydrogen peroxide-based liquid cleaning agent was obtained.

[0153] Comparative Example 2:

[0154] Compared with Example 1, didecyldimethylammonium chloride was not added during the preparation of the hydrogen peroxide-based liquid cleaning agent in this comparative example, and the remaining steps and parameters were the same, which will not be repeated in this comparative example. Finally, a hydrogen peroxide-based liquid cleaning agent was obtained.

[0155] Comparative Example 3:

[0156] Compared with Example 1, the amount of hydrogen peroxide in this comparative example is only adjusted from "0.875g" to "0.5g", and the remaining steps and parameters are the same, which will not be repeated in this comparative example. Finally, a liquid cleaning agent based on hydrogen peroxide is obtained.

[0157] Comparative Example 4:

[0158] Compared with Example 1, this comparative example only adjusts the amount of the composite stabilizer from "0.05g" to "0.02g", and the remaining steps and parameters are the same, which will not be repeated in this comparative example. Finally, a liquid cleaning agent based on hydrogen peroxide is obtained.

[0159] Comparative Example 5:

[0160] Compared with Example 1, the amount of Agent A in this comparative example is adjusted from "60 g" to "30 g", and the remaining steps and parameters are the same, which will not be repeated in this comparative example. Finally, a liquid cleaning agent based on hydrogen peroxide is obtained.

[0161] Comparative Example 6:

[0162] Compared with Example 1, this comparative example only adjusts the amount of the auxiliary enzyme additive from "1g" to "3g", and the remaining steps and parameters are the same, which will not be repeated in this comparative example. Finally, a liquid cleaning agent based on hydrogen peroxide is obtained.

[0163] Performance Test:

[0164] Stability test:

[0165] The hydrogen peroxide content of the liquid cleaning agents prepared in Examples 1 to 6 and Comparative Examples 1 to 6 under room temperature storage conditions was tested, and the retention rates at 1 week, 4 weeks and 8 weeks were calculated;

[0166] Disinfection effect test:

[0167] The sterilization efficiency was tested by the standard suspension method in 2.2.1.4 of the "Standard for Disinfection and Hygiene (2002 Edition)", using the liquid cleaning agents prepared in Examples 1 to 6 and Comparative Examples 1 to 6 and stored for 2 weeks, and the sterilization logarithm values ​​were recorded;

[0168] Cleaning effect test:

[0169] The cleaning effect of the liquid cleaning agents prepared in Examples 1 to 6 and Comparative Examples 1 to 6 was tested with reference to QB / T1803-1993 and GB15979-2002 standards;

[0170] Corrosion resistance test:

[0171] According to Item 2.2.1.2.3 and Item 2.2.1.4 of the Technical Specification for Disinfection (2002 Edition), and with reference to 5.1, 5.2 and 5.4 of GB / T38498-2020 "Method for Evaluation of Metal Corrosion of Disinfectants", the liquid cleaning agents prepared in Examples 1 to 6 and Comparative Examples 1 to 6 of the present invention were tested for their corrosiveness to stainless steel;

[0172] The results are shown in Tables 1 to 3 below:

[0173] Table 1 Summary of stability test results of samples in Examples 1 to 6 and Comparative Examples 1 to 6

[0174]

[0175] Table 2 Summary of experimental results of disinfection effects of samples in Examples 1 to 6 and Comparative Examples 1 to 6

[0176]

[0177] Table 3 Summary of cleaning effect and corrosion resistance test results of samples in Examples 1 to 6 and Comparative Examples 1 to 6

[0178] project Decontamination rate / % Protein residual rate% Corrosion degree / R (mm / a) Example 1 99.5 0.2 0.004637 / basically no corrosion Example 2 98.9 0.4 0.004725 / basically no corrosion Example 3 98.6 0.2 0.004653 / basically no corrosion Example 4 99.2 0.3 0.004815 / basically no corrosion Example 5 99.3 0.3 0.004782 / basically no corrosion Example 6 99.5 0.4 0.004826 / basically no corrosion Comparative Example 1 90.5 2.2 0.005239 / Basically no corrosion Comparative Example 2 92.9 2.4 0.005613 / Basically no corrosion Comparative Example 3 91.6 2.2 0.005239 / Basically no corrosion Comparative Example 4 90.2 2.3 0.005613 / Basically no corrosion Comparative Example 5 92.3 2.3 0.005239 / Basically no corrosion Comparative Example 6 93.5 2.4 0.005613 / Basically no corrosion

[0179] Data Analysis:

[0180] It can be seen from Table 1 that the liquid cleaning agent prepared by the present invention exhibits higher storage stability of hydrogen peroxide; it can be seen from Table 2 that the liquid cleaning agent prepared by the present invention has better disinfection and sterilization effect; it can be seen from Table 3 that the liquid cleaning agent prepared by the present invention has a stronger decontamination rate, a lower protein residual rate and good corrosion resistance;

[0181] This may be because the double-chain quaternary ammonium salt and PCA zinc are introduced into the present invention. During the cleaning process, the double-chain quaternary ammonium salt can neutralize the charges on the surface of bacteria and viruses, promote the aggregation of pathogens, and then accelerate the contact between hydrogen peroxide molecules and pathogens. At the same time, when the double-chain quaternary ammonium salt is adsorbed and consumed, the PCA zinc in the system will be partially hydrolyzed to produce nano zinc oxide, which can activate hydrogen peroxide to decompose and produce hydroxyl free radicals with stronger oxidizing ability, thereby achieving the killing of pathogens in a shorter time.

[0182] In addition, the 4-formylphenylboronic acid introduced into the system can effectively inhibit the activity of the protease during storage, prevent the protease from hydrolyzing itself or being hydrolyzed by other enzymes, and protect the enzyme. The hydrogen peroxide in the compound A agent can slowly restore the enzyme activity during use, so that the enzyme can play its role to the maximum extent, and the propylene glycol anisole therein can assist the dissolution of the additive. The added composite stabilizer and propylene glycol have less ability to form hydrogen bonds than water, and have less destructive power on the hydrogen bond structure of protein molecules. They can replace water as a solvent to stabilize the protease, form a coordination effect to reduce damage to the enzyme, and prevent the reduction of enzyme activity. The introduced composite corrosion inhibitor and corrosion inhibitor can effectively prevent the medical device from being corroded by the cleaning agent while cleaning, disinfecting and sterilizing the medical device, so it has broad application prospects.

[0183] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention is limited to these examples. Under the concept of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0184] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A liquid cleaning agent based on hydrogen peroxide, characterized in that The liquid cleaning agent includes agent A and agent B; The mass ratio of the A agent to the B agent is 60-70:30-40; The agent A is prepared from the following raw materials: hydrogen peroxide, double-chain quaternary ammonium salt, PCA zinc, composite stabilizer, composite corrosion inhibitor and pH regulator; The preparation process of the A agent is as follows: Step A1. Add hydrogen peroxide to deionized water and stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A1; Step A2. Add the composite stabilizer to the mixed solution A1, and stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A2; Step A3. Add the double-chain quaternary ammonium salt to the mixed solution A2, and stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A3; Step A4. Add PCA zinc to the mixed solution A3, and stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A4; Step A5. Add the composite corrosion inhibitor to the mixed solution A4, stir at a speed of 60-90 r / min for 5-7 min to obtain a mixed solution A5; Step A6. Add the pH adjuster to the mixed solution A5, and stir at a speed of 60-90 r / min for 14-16 min to obtain agent A; The composite corrosion inhibitor in step A5 is obtained by mixing thiourea and benzotriazole in a mass ratio of 0.8-1.2:1.8-2.2; The agent B is prepared from the following raw materials: triethanolamine oleic acid soap, benzotriazole, propylene glycol monomethyl ether, glycerol, additives, surfactants, corrosion inhibitors and deionized water; The additive is obtained by mixing a multi-enzyme additive and an auxiliary enzyme additive in a mass ratio of 3-4:1-2; The multi-enzyme additive is prepared from the following raw materials: calcium chloride, 4-formylphenylboric acid, propylene glycol monomethyl ether, glycerol and composite enzyme; The mass ratio of hydrogen peroxide to deionized water in step A1 is 0.875-1.15:80-85; The mass ratio of the composite stabilizer to the mixed solution A1 in step A2 is 0.05-0.15:80-85; The composite stabilizer in step A2 is obtained by mixing sodium pyrophosphate and hydroxyethylidene diphosphonic acid in a mass ratio of 1.8-2.2:0.8-1.2; The mass ratio of the double-chain quaternary ammonium salt to the mixed solution A2 in step A3 is 0.18-0.22:80-85; The double-chain quaternary ammonium salt in step A3 is any one of didecyldimethylammonium chloride, didecyldimethylammonium bromide, octyldecyldimethylammonium chloride, octyldecyldimethylammonium bromide, dioctyldimethylammonium chloride and dioctyldimethylammonium bromide; The mass ratio of PCA zinc to mixed solution A3 in step A4 is 0.6-1.5:80-85.

2. The hydrogen peroxide-based liquid cleaning agent according to claim 1, characterized in that The mass ratio of the composite corrosion inhibitor to the mixed solution A4 in step A5 is 0.08-0.2:80-85; The mass ratio of the pH adjuster to the mixed solution A5 in step A6 is 0.1-0.2:80-85; The pH adjuster in step A6 is any one of phosphoric acid and sodium phosphate.

3. The hydrogen peroxide-based liquid cleaning agent according to claim 1, characterized in that The preparation process of the B agent is as follows: Step B1. Evenly mixing the multi-enzyme additive and the auxiliary enzyme additive to obtain an additive; Step B2. Disperse triethanolamine oleate soap, benzotriazole, propylene glycol monomethyl ether, glycerol and additives in deionized water and mix well to obtain a mixed solution B1; Step B3. Add a surfactant and a corrosion inhibitor to the mixed solution B1, mix well, and obtain agent B.

4. The hydrogen peroxide-based liquid cleaning agent according to claim 3, characterized in that The preparation process of the multi-enzyme additive in step B1 is as follows: calcium chloride, 4-formylphenylboric acid, propylene glycol monomethyl ether, glycerol and composite enzyme are mixed, and stirred at 25-30° C. for 30-35 minutes to obtain the multi-enzyme additive; The complex enzyme is obtained by mixing protease, lipase, xylitolase, cellulase and amylase in a mass ratio of 10-12:4-6:1-3:0.8-1.2:0.8-1.2; The auxiliary enzyme additive in step B1 is obtained by mixing lysozyme, amylase and lipase in a mass ratio of 6-9:2-5:2-6.

5. The hydrogen peroxide-based liquid cleaning agent according to claim 3, characterized in that The mass ratio of triethanolamine oleate soap, benzotriazole, propylene glycol monomethyl ether, glycerol, additives and deionized water in step B2 is 8-12:0.4-0.6:1-2:1-1.5:3-5:54.9-67.6; The mass ratio of the mixed solution B1, the surfactant and the corrosion inhibitor in step B3 is 66.9-86.6:16-20:2.5-4.

6. The hydrogen peroxide-based liquid cleaning agent according to claim 3, characterized in that The surfactant in step B3 is obtained by mixing triethanolamine oleic acid soap and AEO-9 in a mass ratio of 8-12:38-42; The corrosion inhibitor in step B3 is sodium gluconate.

7. A method for preparing a hydrogen peroxide-based liquid cleaning agent according to any one of claims 1 to 6, characterized in that: The following steps are involved: Mix Agent A and Agent B evenly to obtain a hydrogen peroxide-based liquid cleaning agent.

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