Antibacterial gel containing flavonoids and preparation method thereof

Abstract

The invention relates to the technical field of antibacterial gels, and discloses a flavonoid-containing antibacterial gel and a preparation method thereof.The preparation method comprises the steps that carboxymethyl chitosan cross-linked nano sodium hyaluronate, quaternary ammonium salt modified sodium alginate, polyvinyl alcohol, flavonol glycosides, flavanonol and gelatin are added into a stirrer and stirred, and a mixture is obtained; after the reaction is finished, the antibacterial gel containing the flavonoids is obtained. Quaternary ammonium salt groups in the antibacterial gel can destroy cell membranes, enable cellular contents to leak and cause cell death, so that the antibacterial effect is achieved, sodium alginate has more reaction sites, a large amount of quaternary ammonium salt is grafted through reaction, the substitution degree of the quaternary ammonium salt is increased, the antibacterial effect is further improved, and the antibacterial gel has a good antibacterial effect. The sodium alginate and the cardanol have a certain antibacterial effect, so that an antibacterial system is formed by the sodium alginate and the cardanol together.

Description

Technical Field

[0001] The invention relates to the technical field of antibacterial gel, in particular to an antibacterial gel containing flavonoids and a preparation method thereof. Background Art

[0002] At present, there are many kinds of antibacterial products on the market, most of which are antibacterial washes, which have shortcomings such as short administration time and insufficient drug effect. Therefore, how to avoid this phenomenon is the key to solving the problem. For example, patent CNC101766561B discloses an antibacterial gel and its preparation method. The invention discloses an antibacterial gel with definite efficacy and low toxicity and side effects after matrix configuration and ingredient preparation. The preparation method mainly kills pathogens such as Staphylococcus aureus, Candida albicans, and Escherichia coli that cause gynecological inflammation. However, its bactericidal ingredients are single and its application range is narrow. Summary of the invention

[0003] 1. Technical issues to be resolved

[0004] In view of the deficiencies of the prior art, the present invention provides an antibacterial gel containing flavonoids and a preparation method thereof, which has good antibacterial effect.

[0005] (II) Technical solution

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: an antibacterial gel containing flavonoids and a preparation method thereof, an antibacterial gel containing flavonoids, comprising the following weight components: 16-25 weight parts of carboxymethyl chitosan cross-linked nano hyaluronate sodium, 10-15 weight parts of quaternary ammonium salt modified sodium alginate, 8-15 weight parts of polyvinyl alcohol, 6-10 weight parts of flavonol glycosides, 4-8 weight parts of dihydroflavonol, and 10-16 weight parts of gelatin;

[0007] The preparation method of the carboxymethyl chitosan cross-linked nano sodium hyaluronate is:

[0008] S1. Add carboxymethyl chitosan to N,N-dimethylformamide solvent, add thionyl chloride after stirring evenly, react at 70-90°C for 5-8h, then continue to add sodium hyaluronate dissolved in N,N-dimethylformamide, react for 4-6h, add acetone to precipitate after the reaction, filter the solvent, wash with acetone to obtain carboxymethyl chitosan cross-linked nano sodium hyaluronate; wherein the mass ratio of carboxymethyl chitosan, thionyl chloride and sodium hyaluronate is 1.1-1.3:1:0.9-1.2.

[0009] Preferably, the preparation method of the quaternary ammonium salt modified sodium alginate is:

[0010] (1) adding sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid into a reactor filled with distilled water, stirring to dissolve, stirring and reacting at 30-40° C. for 20-25 hours, transferring to a dialysis bag after the reaction, dialyzing in deionized water for 3-4 days, and drying to obtain phenylboronic acid-modified sodium alginate;

[0011] (2) adding 1,3-bis(dimethylamino)-2-propanol and phenylboric acid-modified sodium alginate to N,N-dimethylformamide solvent, reacting at 60-80° C. for 7-12 hours, and then distilling under reduced pressure, washing and drying to obtain tertiary amine sodium alginate;

[0012] (3) adding 2,2-bis(bromomethyl)-1,3-propylene glycol and cardanol to an acetone solvent, stirring evenly, and then continuing to add a 4-6 mol / L sodium hydroxide aqueous solution, reacting at 65-80° C. for 3-5 hours. After the reaction is completed, neutralizing, concentrating, filtering, washing and drying to obtain dihydroxy cardanol;

[0013] (4) Add dihydroxy cardanol and chloroacetyl chloride to a reactor containing N,N-dimethylformamide solvent, stir and mix, continue to add triethylamine catalyst, react at 70-90° C. for 4-8 hours, and then continue to add tertiary amine sodium alginate to carry out quaternization reaction, react at 80-95° C. for 5-7 hours, and then rotary distill and wash to obtain quaternary ammonium salt modified sodium alginate.

[0014] Preferably, the mass ratio of sodium alginate, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 3-aminophenylboronic acid in (1) is 1:0.01-0.03:0.6-0.8.

[0015] Preferably, the mass ratio of 1,3-bis(dimethylamino)-2-propanol to phenylboronic acid-modified sodium alginate in (2) is 0.8-1.1:1.

[0016] Preferably, the mass ratio of 2,2-bis(bromomethyl)-1,3-propanediol to cardanol in (3) is 1:1.6-2.2.

[0017] Preferably, the mass ratio of dihydroxycardanol, chloroacetyl chloride, triethylamine catalyst and tertiary amino sodium alginate in (4) is 1:1.2-1.6:0.02-0.04:1.1-1.2.

[0018] Preferably, the preparation method of the antibacterial gel containing flavonoids is: add carboxymethyl chitosan cross-linked nano sodium hyaluronate, quaternary ammonium salt modified sodium alginate, polyvinyl alcohol, flavonol glycosides, dihydroflavonol, and gelatin into a stirrer, and stir for 10-16 minutes to obtain the antibacterial gel containing flavonoids.

[0019] (III) Beneficial technical effects

[0020] The invention adds carboxymethyl chitosan cross-linked nano sodium hyaluronate, quaternary ammonium salt modified sodium alginate, polyvinyl alcohol, flavonol glycosides, dihydroflavonol and gelatin into a stirrer, and stirs to obtain the antibacterial gel containing flavonoids.

[0021] Sodium alginate is reacted with 3-aminophenylboronic acid to obtain phenylboronic acid modified sodium alginate, and the hydroxyl group in 1,3-bis(dimethylamino)-2-propanol and the boric acid group in phenylboronic acid modified sodium alginate are reacted to introduce a tertiary amine group, thereby obtaining tertiary amine sodium alginate; the bromine in 2,2-bis(bromomethyl)-1,3-propanediol and the phenolic hydroxyl group in cardanol are subjected to a substitution reaction to introduce a hydroxyl group, thereby obtaining dihydroxy cardanol; dihydroxy cardanol is reacted with chloroacetyl chloride, and then quaternized with tertiary amine sodium alginate to obtain quaternary ammonium salt modified sodium alginate.

[0022] The quaternary ammonium salt groups in the antibacterial gel will destroy the cell membrane, causing the cell contents to leak and leading to cell death, thereby achieving an antibacterial effect. The sodium alginate has many reaction sites, and a large amount of quaternary ammonium salts are grafted through the reaction, which increases the degree of substitution of the quaternary ammonium salts and further improves its antibacterial effect. In addition, sodium alginate and cardanol themselves have certain antibacterial effects, and together they constitute an antibacterial system. The flavonoids therein also have good antibacterial effects. DETAILED DESCRIPTION

[0023] Example 1

[0024] (1) adding sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid into a reactor filled with distilled water, stirring and dissolving, wherein the mass ratio of sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid is 1:0.01:0.6, stirring and reacting at 30° C. for 20 hours, transferring to a dialysis bag after completion, dialyzing in deionized water for 3 days, and drying to obtain phenylboronic acid-modified sodium alginate;

[0025] (2) adding 1,3-bis(dimethylamino)-2-propanol and phenylboric acid-modified sodium alginate to N,N-dimethylformamide solvent, wherein the mass ratio of 1,3-bis(dimethylamino)-2-propanol to phenylboric acid-modified sodium alginate is 0.8:1, reacting at 60° C. for 7 h, and then distilling under reduced pressure, washing and drying to obtain tertiary amine sodium alginate;

[0026] (3) adding 2,2-bis(bromomethyl)-1,3-propylene glycol and cardanol to an acetone solvent, wherein the mass ratio of 2,2-bis(bromomethyl)-1,3-propylene glycol to cardanol is 1:1.6, stirring evenly, and then continuing to add 4 mol / L sodium hydroxide aqueous solution, reacting at 65° C. for 3 h. After the reaction is completed, neutralizing, concentrating, filtering, washing and drying to obtain dihydroxy cardanol;

[0027] (4) adding dihydroxy cardanol and chloroacetyl chloride to a reactor containing N,N-dimethylformamide solvent, stirring and mixing, continuing to add triethylamine catalyst, reacting at 70° C. for 4 hours, and then continuing to add tertiary amine sodium alginate to carry out quaternization reaction, reacting at 80° C. for 5 hours, wherein the mass ratio of dihydroxy cardanol, chloroacetyl chloride, triethylamine catalyst, and tertiary amine sodium alginate is 1:1.2:0.02:1.1, and after completion, rotary distillation and washing are performed to obtain quaternary ammonium salt modified sodium alginate;

[0028] (5) adding carboxymethyl chitosan to N,N-dimethylformamide solvent, adding thionyl chloride after stirring evenly, reacting at 70° C. for 5 h, and then continuing to add sodium hyaluronate dissolved in N,N-dimethylformamide, wherein the mass ratio of carboxymethyl chitosan, thionyl chloride, and sodium hyaluronate is 1.1:1:0.9, reacting for 4 h, adding acetone after the reaction to precipitate, filtering the solvent, washing with acetone, and obtaining carboxymethyl chitosan cross-linked nano sodium hyaluronate;

[0029] (6) 16 parts by weight of carboxymethyl chitosan cross-linked nano-sodium hyaluronate, 10 parts by weight of quaternary ammonium salt modified sodium alginate, 8 parts by weight of polyvinyl alcohol, 6 parts by weight of flavonol glycosides, 4 parts by weight of dihydroflavonol, and 10 parts by weight of gelatin are added into a blender and stirred for 10 minutes to obtain an antibacterial gel containing flavonoids.

[0030] Example 2

[0031] (1) adding sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid into a reactor filled with distilled water, stirring and dissolving, wherein the mass ratio of sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid is 1:0.03:0.8, stirring and reacting at 40° C. for 25 hours, transferring to a dialysis bag after the reaction, dialyzing in deionized water for 4 days, and drying to obtain phenylboronic acid-modified sodium alginate;

[0032] (2) adding 1,3-bis(dimethylamino)-2-propanol and phenylboric acid-modified sodium alginate to N,N-dimethylformamide solvent, wherein the mass ratio of 1,3-bis(dimethylamino)-2-propanol to phenylboric acid-modified sodium alginate is 1.1:1, reacting at 80° C. for 12 h, and then performing reduced pressure distillation after the reaction, washing and drying to obtain tertiary amine sodium alginate;

[0033] (3) adding 2,2-bis(bromomethyl)-1,3-propylene glycol and cardanol to an acetone solvent, wherein the mass ratio of 2,2-bis(bromomethyl)-1,3-propylene glycol to cardanol is 1:2.2, stirring evenly, and then continuing to add a 6 mol / L sodium hydroxide aqueous solution, reacting at 65-80° C. for 5 h. After the reaction is completed, neutralizing, concentrating, filtering, washing and drying to obtain dihydroxy cardanol;

[0034] (4) adding dihydroxy cardanol and chloroacetyl chloride to a reactor containing N,N-dimethylformamide solvent, stirring and mixing, continuing to add triethylamine catalyst, reacting at 90° C. for 8 h, and then continuing to add tertiary amine sodium alginate to carry out quaternization reaction, reacting at 95° C. for 7 h, wherein the mass ratio of dihydroxy cardanol, chloroacetyl chloride, triethylamine catalyst, and tertiary amine sodium alginate is 1:1.6:0.04:1.2, and after completion, rotary distillation and washing are performed to obtain quaternary ammonium salt modified sodium alginate;

[0035] (5) adding carboxymethyl chitosan to N,N-dimethylformamide solvent, adding thionyl chloride after stirring evenly, reacting at 90° C. for 8 h, and then continuing to add sodium hyaluronate dissolved in N,N-dimethylformamide, wherein the mass ratio of carboxymethyl chitosan, thionyl chloride and sodium hyaluronate is 1.3:1:1.2, reacting for 6 h, adding acetone after the reaction to precipitate, filtering the solvent, washing with acetone, and obtaining carboxymethyl chitosan cross-linked nano sodium hyaluronate;

[0036] (6) 25 parts by weight of carboxymethyl chitosan cross-linked nano-sodium hyaluronate, 15 parts by weight of quaternary ammonium salt modified sodium alginate, 15 parts by weight of polyvinyl alcohol, 10 parts by weight of flavonol glycosides, 8 parts by weight of dihydroflavonol, and 16 parts by weight of gelatin are added into a blender and stirred for 16 minutes to obtain an antibacterial gel containing flavonoids.

[0037] Example 3

[0038] (1) adding sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid into a reactor filled with distilled water, stirring and dissolving, wherein the mass ratio of sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid is 1:0.02:0.7, stirring and reacting at 35° C. for 22 hours, transferring to a dialysis bag after completion, dialyzing in deionized water for 3 days, and drying to obtain phenylboronic acid-modified sodium alginate;

[0039] (2) adding 1,3-bis(dimethylamino)-2-propanol and phenylboric acid-modified sodium alginate to N,N-dimethylformamide solvent, wherein the mass ratio of 1,3-bis(dimethylamino)-2-propanol to phenylboric acid-modified sodium alginate is 0.9:1, reacting at 70° C. for 9 hours, and then distilling under reduced pressure, washing and drying to obtain tertiary amine sodium alginate;

[0040] (3) adding 2,2-bis(bromomethyl)-1,3-propylene glycol and cardanol to an acetone solvent, wherein the mass ratio of 2,2-bis(bromomethyl)-1,3-propylene glycol to cardanol is 1:1.9, stirring evenly, and then continuing to add 5 mol / L sodium hydroxide aqueous solution, reacting at 72° C. for 4 h. After the reaction is completed, neutralizing, concentrating, filtering, washing and drying to obtain dihydroxy cardanol;

[0041] (4) adding dihydroxy cardanol and chloroacetyl chloride to a reactor containing N,N-dimethylformamide solvent, stirring and mixing, continuing to add triethylamine catalyst, reacting at 80° C. for 6 h, and then continuing to add tertiary amine sodium alginate to carry out quaternization reaction, reacting at 87° C. for 6 h, wherein the mass ratio of dihydroxy cardanol, chloroacetyl chloride, triethylamine catalyst, and tertiary amine sodium alginate is 1:1.4:0.03:1.15, and after completion, rotary distillation and washing are performed to obtain quaternary ammonium salt modified sodium alginate;

[0042] (5) adding carboxymethyl chitosan to N,N-dimethylformamide solvent, adding thionyl chloride after stirring evenly, reacting at 80° C. for 6 h, and then continuing to add sodium hyaluronate dissolved in N,N-dimethylformamide, wherein the mass ratio of carboxymethyl chitosan, thionyl chloride and sodium hyaluronate is 1.2:1:1.1, reacting for 5 h, adding acetone after the reaction to precipitate, filtering the solvent, washing with acetone, and obtaining carboxymethyl chitosan cross-linked nano sodium hyaluronate;

[0043] (6) 20 parts by weight of carboxymethyl chitosan cross-linked nano-sodium hyaluronate, 12 parts by weight of quaternary ammonium salt modified sodium alginate, 11 parts by weight of polyvinyl alcohol, 8 parts by weight of flavonol glycosides, 6 parts by weight of dihydroflavonol, and 13 parts by weight of gelatin are added into a blender and stirred for 13 minutes to obtain an antibacterial gel containing flavonoids.

[0044] Example 4

[0045] (1) adding sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid into a reactor filled with distilled water, stirring and dissolving, wherein the mass ratio of sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid is 1:0.03:0.8, stirring and reacting at 40° C. for 25 hours, transferring to a dialysis bag after the reaction, dialyzing in deionized water for 4 days, and drying to obtain phenylboronic acid-modified sodium alginate;

[0046] (2) adding 1,3-bis(dimethylamino)-2-propanol and phenylboric acid-modified sodium alginate to N,N-dimethylformamide solvent, wherein the mass ratio of 1,3-bis(dimethylamino)-2-propanol to phenylboric acid-modified sodium alginate is 1.1:1, reacting at 80° C. for 12 h, and then performing reduced pressure distillation after the reaction, washing and drying to obtain tertiary amine sodium alginate;

[0047] (3) adding 2,2-bis(bromomethyl)-1,3-propylene glycol and cardanol to an acetone solvent, wherein the mass ratio of 2,2-bis(bromomethyl)-1,3-propylene glycol to cardanol is 1:1.9, stirring evenly, and then continuing to add 5 mol / L sodium hydroxide aqueous solution, reacting at 72° C. for 4 h. After the reaction is completed, neutralizing, concentrating, filtering, washing and drying to obtain dihydroxy cardanol;

[0048] (4) adding dihydroxy cardanol and chloroacetyl chloride to a reactor containing N,N-dimethylformamide solvent, stirring and mixing, continuing to add triethylamine catalyst, reacting at 80° C. for 6 h, and then continuing to add tertiary amine sodium alginate to carry out quaternization reaction, reacting at 87° C. for 6 h, wherein the mass ratio of dihydroxy cardanol, chloroacetyl chloride, triethylamine catalyst, and tertiary amine sodium alginate is 1:1.4:0.03:1.15, and after completion, rotary distillation and washing are performed to obtain quaternary ammonium salt modified sodium alginate;

[0049] (5) adding carboxymethyl chitosan to N,N-dimethylformamide solvent, adding thionyl chloride after stirring evenly, reacting at 70° C. for 5 h, and then continuing to add sodium hyaluronate dissolved in N,N-dimethylformamide; wherein the mass ratio of carboxymethyl chitosan, thionyl chloride, and sodium hyaluronate is 1.2:1:1.1, reacting for 4 h, adding acetone after the reaction to precipitate, filtering the solvent, washing with acetone, and obtaining carboxymethyl chitosan cross-linked nano sodium hyaluronate;

[0050] (6) 16 parts by weight of carboxymethyl chitosan cross-linked nano-sodium hyaluronate, 10 parts by weight of quaternary ammonium salt modified sodium alginate, 8 parts by weight of polyvinyl alcohol, 6 parts by weight of flavonol glycosides, 4 parts by weight of dihydroflavonol, and 10 parts by weight of gelatin are added into a blender and stirred for 10 minutes to obtain an antibacterial gel containing flavonoids.

[0051] Comparative Example 1

[0052] 16 parts by weight of carboxymethyl chitosan cross-linked nano-sodium hyaluronate, 8 parts by weight of polyvinyl alcohol, 6 parts by weight of flavonol glycosides, 4 parts by weight of dihydroflavonol, and 10 parts by weight of gelatin are added into a blender and stirred for 10 minutes to obtain an antibacterial gel containing flavonoids.

[0053] Comparative Example 2

[0054] 10 parts by weight of quaternary ammonium salt-modified sodium alginate, 8 parts by weight of polyvinyl alcohol, 6 parts by weight of flavonol glycosides, 4 parts by weight of dihydroflavonol, and 10 parts by weight of gelatin are added into a stirrer and stirred for 10 minutes to obtain an antibacterial gel containing flavonoids.

[0055] Using Staphylococcus aureus, Escherichia coli or Candida albicans as the test bacteria, 0.5 mL of the bacterial suspension of the test bacteria (concentration of 1×110 CFU / mL) was added to a sterile culture dish containing MH broth medium, and then the antibacterial gel sample containing flavonoids was attached to the surface of the culture medium, and cultured in a constant temperature incubator at 35°C for 18 h. After culture, the diameter of the inhibition zone was measured.

[0056] Table 1: Antimicrobial testing.

[0057]

[0058]

[0059] It can be seen from Table 1 that Examples 1-4 of the present invention have better antibacterial function than Comparative Examples 1-2.

[0060] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. An antibacterial gel containing flavonoids, characterized in that: The invention comprises the following components by weight: 16-25 parts by weight of carboxymethyl chitosan cross-linked nano sodium hyaluronate, 10-15 parts by weight of quaternary ammonium salt modified sodium alginate, 8-15 parts by weight of polyvinyl alcohol, 6-10 parts by weight of flavonol glycosides, 4-8 parts by weight of dihydroflavonol, and 10-16 parts by weight of gelatin; The preparation method of the carboxymethyl chitosan cross-linked nano sodium hyaluronate is: S1. Add carboxymethyl chitosan to N,N-dimethylformamide solvent, add thionyl chloride after stirring evenly, react at 70-90°C for 5-8h, then continue to add sodium hyaluronate dissolved in N,N-dimethylformamide, react for 4-6h, add acetone to precipitate after the reaction, filter the solvent, wash with acetone to obtain carboxymethyl chitosan cross-linked nano sodium hyaluronate; wherein the mass ratio of carboxymethyl chitosan, thionyl chloride and sodium hyaluronate is 1.1-1.3:1:0.9-1.

2.

2. The antibacterial gel containing flavonoids according to claim 1, characterized in that: The preparation method of the quaternary ammonium salt modified sodium alginate is: (1) adding sodium alginate, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and 3-aminophenylboronic acid into a reactor filled with distilled water, stirring to dissolve, stirring and reacting at 30-40° C. for 20-25 hours, transferring to a dialysis bag after the reaction, dialyzing in deionized water for 3-4 days, and drying to obtain phenylboronic acid-modified sodium alginate; (2) adding 1,3-bis(dimethylamino)-2-propanol and phenylboric acid-modified sodium alginate to N,N-dimethylformamide solvent, reacting at 60-80° C. for 7-12 hours, and then distilling under reduced pressure, washing and drying to obtain tertiary amine sodium alginate; (3) adding 2,2-bis(bromomethyl)-1,3-propylene glycol and cardanol to an acetone solvent, stirring evenly, and then continuing to add a 4-6 mol / L sodium hydroxide aqueous solution, reacting at 65-80° C. for 3-5 hours. After the reaction is completed, neutralizing, concentrating, filtering, washing and drying to obtain dihydroxy cardanol; (4) Add dihydroxy cardanol and chloroacetyl chloride to a reactor containing N,N-dimethylformamide solvent, stir and mix, continue to add triethylamine catalyst, react at 70-90° C. for 4-8 hours, and then continue to add tertiary amine sodium alginate to carry out quaternization reaction, react at 80-95° C. for 5-7 hours, and then rotary distill and wash to obtain quaternary ammonium salt modified sodium alginate.

3. The antibacterial gel containing flavonoids according to claim 2, characterized in that: The mass ratio of sodium alginate, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 3-aminophenylboronic acid in (1) is 1:0.01-0.03:0.6-0.

8.

4. The antibacterial gel containing flavonoids according to claim 2, characterized in that: The mass ratio of 1,3-bis(dimethylamino)-2-propanol to phenylboronic acid-modified sodium alginate in (2) is 0.8-1.1:

1.

5. The antibacterial gel containing flavonoids according to claim 2, characterized in that: The mass ratio of 2,2-bis(bromomethyl)-1,3-propanediol to cardanol in (3) is 1:1.6-2.

2.

6. The antibacterial gel containing flavonoids according to claim 2, characterized in that: The mass ratio of dihydroxy cardanol, chloroacetyl chloride, triethylamine catalyst and tertiary amino sodium alginate in (4) is 1:1.2-1.6:0.02-0.04:1.1-1.

2.

7. A method for preparing the antibacterial gel containing flavonoids according to any one of claims 1 to 6, characterized in that: The preparation method of the antibacterial gel containing flavonoids is as follows: carboxymethyl chitosan cross-linked nano sodium hyaluronate, quaternary ammonium salt modified sodium alginate, polyvinyl alcohol, flavonol glycosides, dihydroflavonol, and gelatin are added into a stirrer, and stirred for 10-16 minutes to obtain the antibacterial gel containing flavonoids.

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