Microorganism culture integrated equipment

Abstract

The invention relates to the technical field of microorganism culture, in particular to microorganism culture integrated equipment which comprises a shell and a cover plate, a stand column is coaxially and fixedly installed in the middle of the shell, the cover plate is detachably and rotatably installed on the stand column, a culture cavity is formed between the shell and the cover plate, and a heating plate used for heating the culture cavity is arranged at the bottom in the shell. A round tray is coaxially and fixedly installed on the stand column, a plurality of round grooves are formed in the tray, a sampling opening used for inserting a rubber head dropper is formed in the cover plate, a sealing assembly used for sealing the sampling opening is arranged on the shell, and a plurality of sampling assemblies used for assisting sampling are further arranged on the tray. The sampling port is formed in the cover plate, the rubber head dropper extends into the culture cavity during sampling, the rubber head dropper is used for sealing the sampling port, temperature loss in the culture cavity in the sampling process is avoided, the structure is simple, and the cover plate can rotate to facilitate sampling of all culture dishes in the culture cavity.

Description

Technical Field

[0001] The invention relates to the technical field of microorganism cultivation devices, in particular to integrated microorganism cultivation equipment. Background Art

[0002] Microbial culture refers to the rapid growth and reproduction of certain microorganisms with the help of artificially prepared culture media and artificially created culture conditions. Microbial culture is often required in food production, pharmaceutical and biological laboratory experiments. The reproduction of microorganisms requires a specific environment. In order to increase the yield of microorganisms and ensure the purity of microorganisms, they are generally cultured in microbial culture equipment, such as an incubator.

[0003] During the microbial cultivation process, in order to ensure the speed of microbial reproduction, the temperature inside the incubator is generally set to the temperature with the highest microbial activity and maintained at a constant temperature. However, during the microbial cultivation process, it is necessary to regularly take out the microbial culture dishes inside the incubator for sampling and observation to ensure the survival of the microorganisms and avoid the occurrence of large-scale deaths of microorganisms. In the process of taking out the microorganisms, the incubator needs to be opened, which can easily cause the temperature inside the incubator to change. After the temperature changes, the activity of the microorganisms will decrease, and it is necessary to wait for the temperature in the box to return to the temperature most suitable for microbial growth. In this way, the efficiency of microbial cultivation will be reduced, and temperature changes may also cause partial or large-scale deaths of microorganisms.

[0004] In order to reduce the temperature change amplitude inside the microbial incubator during the process of microbial sampling and detection, and avoid the reduction of microbial activity or even death. The Chinese invention patent with application number: CN202211440781.7 and the name of "An integrated device for microbial culture and reproduction" proposes a solution, including a box body, a heating plate is fixedly installed inside the box body, and a first box cover is hinged on the front wall of the box body; a support plate is rotatably installed on the inner bottom wall of the box body, and a motor is fixedly installed on the bottom wall of the box body, and the output end of the motor extends into the box body and is fixedly connected to the rotating shaft of the support plate; a device box connected to the box body is fixedly inserted on the side wall of the box body, and a placement port is opened on the front wall of the device box. A second box cover that matches the placement port is hinged on the front wall of the device box. Under the action of the device box, the first heat conduction box, the second telescopic plate, and the second telescopic rod, the heat loss in the box body can be reduced, which plays a role in ensuring the activity of microorganisms.

[0005] The above patent uses components such as motors and telescopic rods to push the microbial culture dish into a separate chamber and then take it out. Although it solves the problem of temperature changes inside the incubator caused by microbial sampling to a certain extent, the control is complex and the manufacturing cost is high. The use of components such as motors and push rods will make the equipment larger and difficult to repair after a failure. It is suitable for large-scale microbial culture, but not suitable for small-batch microbial culture experiments.

[0006] To this end, an integrated microbial cultivation device is proposed. Summary of the invention

[0007] The object of the present invention is to provide an integrated microbial culture device, which can reduce the impact of the temperature of the microbial culture environment during the sampling process through a relatively simple structure, so as to facilitate experimenters to carry out microbial culture experiments.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A microbial culture integrated device includes a shell and a cover plate, wherein the shell is cylindrical, a column is coaxially fixedly installed in the middle of the shell, the cover plate is detachably rotatably installed on the column for closing the opening of the shell, a culture chamber is formed between the shell and the cover plate, a heating plate for heating the culture chamber is provided at the bottom of the shell, a circular tray is coaxially fixedly installed on the column, the tray is located above the heating plate and does not contact the heating plate, a plurality of circular grooves for storing culture dishes are provided on the tray, the plurality of grooves are evenly distributed on the tray with the center of the tray as the reference circumference, and the centers of the plurality of grooves are all located on the same circumference with the center of the tray as the center. A sampling port for inserting a rubber-tipped dropper is provided on the cover plate, the sampling port is a circular port, the size of the sampling port is adapted to the rubber-tipped dropper, the center of the sampling port is on the same circumference as the center of the groove in the vertical direction, and a sealing component for sealing the sampling port is provided on the shell.

[0010] The cover plate and the closing component close the culture chamber to prevent the temperature inside the culture chamber from being lost and to keep the inside of the culture chamber at a constant temperature. When sampling, the closing component opens the sampling port, and a rubber-tipped dropper can be inserted from the sampling port into the culture chamber to absorb the microorganisms in the culture dish, so as to perform sampling and testing and observe the culture conditions of the microorganisms. When sampling, the sampling port is small, and the rubber-tipped dropper occupies the space of the sampling port when it is inserted into the culture chamber. The rubber-tipped dropper closes the sampling port again, preventing the heat inside the culture chamber from being lost during the sampling process. The cover plate is rotatably installed on the outer shell, and the sampling port can be aligned with each groove by rotating the cover plate, so as to facilitate sampling of each microbial culture dish in the culture chamber. The structure is simple and easy to use and maintain.

[0011] Preferably, the tray is also provided with a plurality of sampling components for auxiliary sampling, and the plurality of sampling components correspond to the plurality of grooves one by one. The sampling components include a bracket arranged above each groove, the brackets are all clamped on the tray, the brackets are all across the corresponding grooves, the brackets are all fixed with cylindrical sleeves, the sleeves are all coaxially arranged with the grooves, the center of the sleeves are all coaxially slidably installed with extension tubes, the upper end of the extension tube is used to dock with the rubber-tipped dropper, and the lower side of the extension tube is evenly distributed with multiple branch tubes, and the multiple branch tubes are connected to the extension tube. The branch tubes distributed circumferentially at the bottom of the extension tube enable the rubber-tipped dropper to be inserted into the top of the extension tube, and then the solution at different positions inside the culture dish can be sucked into the culture dish through negative pressure, thereby reducing the limitations of the rubber-tipped dropper when sampling, so that the sampling can better reflect the growth of microorganisms inside the culture dish, thereby improving the accuracy of sampling detection, and helping to make adjustments to the subsequent microbial culture plan according to the actual situation of sampling detection.

[0012] Preferably, a support is provided at the upper end of the extension tube, a spring groove is coaxially provided on the sleeve, a return spring is provided inside the spring groove, one end of the return spring abuts against the bottom of the spring groove, and the other end abuts against the bottom of the support, the return spring is used to keep the extension tube lifted upward, a first threaded portion is provided on the extension tube, a second threaded portion that cooperates with the first threaded portion is provided on the sleeve, the extension tube is meshed with the second threaded portion on the sleeve through the first threaded portion, and the thread lead angles of the first threaded portion and the second threaded portion are both greater than the equivalent friction angle. The meshing between the first thread and the second thread is not self-locking, so that the extension tube can rotate along the second thread while moving axially after being subjected to the pressure of the axial force. When sampling, squeezing the extension tube downward with a rubber-tipped dropper can drive the extension tube to rotate, and the extension tube can drive the branch tube to rotate together after the extension tube rotates, and the rotation of the branch tube can disturb the microorganisms and the culture solution inside the culture dish, so that the microorganisms can be evenly distributed in the culture solution, improve the microorganism's intake efficiency of the nutrient solution, and then improve the growth rate of the microorganism. In addition, after the branch pipe disturbs the nutrient solution inside the culture dish, the sampling is further made more uniform, and the sampling can better reflect the growth of microorganisms inside the culture dish, thereby improving the accuracy of sampling.

[0013] Preferably, stirring blades are fixedly mounted on the branch pipes, and the stirring blades are at an angle of 30° to 60° with the horizontal plane. The provision of the stirring blades can further improve the stirring effect of the culture solution in the culture dish when the branch pipe rotates. The stirring blades are arranged at an angle of 30° to 60° with the horizontal plane. Compared with making the stirring blades vertical, it can avoid the stirring blades from excessively pushing the culture solution inside the culture dish toward the edge of the culture dish when the extension tube rotates, and avoid splashing the culture solution outside the culture dish. In addition, the stirring blades are arranged at an angle of 30° to 60° with the horizontal plane, which can better improve the up and down disturbance effect of the culture solution during the rotation of the stirring blades, help to evenly distribute the microorganisms in the nutrient solution, improve the contact between the microorganisms and the nutrient solution, and thus improve the growth efficiency of the microorganisms.

[0014] Preferably, the sleeve is provided with a slot, a ball is arranged inside the slot, a locking spring is arranged inside the slot, the locking spring is located between the bottom of the slot and the ball and is used to push the ball out of the slot, the center of all the balls is located on the same horizontal circumference with the center of the tray as the reference, the bottom of the cover plate is provided with a locking slot matching the ball, the locking slot is located on the same circumference as the ball in the vertical direction, when the ball is inserted into the locking slot, the sampling port is aligned with the extension tube on the sleeve where the ball is located. The cover plate is rotated, when the locking slot on the cover plate passes the ball, the ball is pushed into the locking slot under the action of the locking spring, and the extension tube on the sleeve where the ball is located is aligned with the sampling port, so that the user can insert the rubber-tipped dropper from the sampling port into the culture chamber and dock with the extension tube. At the same time, it also avoids the rotation of the cover plate when the rubber-tipped dropper docks with the extension tube during sampling, resulting in the dislocation and burst damage of the rubber-tipped dropper and the extension tube, thereby improving the stability and safety of the equipment during use.

[0015] Preferably, the sealing component includes a crossbeam fixedly mounted inside the culture chamber, one end of the crossbeam is fixedly mounted on the inner wall of the shell, and the other end is fixedly mounted on the column, the crossbeam and the groove are staggered in the vertical direction, the crossbeam fits the bottom of the cover plate, and when the sampling port is located directly above the crossbeam, the crossbeam seals the sampling port. The crossbeam seals the sampling port to prevent the temperature inside the culture chamber from being lost from the sampling port to the outside of the culture chamber, ensuring that the temperature of the growth environment of the microorganisms is in the optimal state. In addition, the crossbeam is supported at the bottom of the cover plate to support the cover plate, further improving the stability of the cover plate when it rotates.

[0016] Preferably, the cover is made of a transparent material, and a soft layer is provided on the top of the crossbeam, and the soft layer is bonded to the bottom of the cover. The cover is made of a transparent material, which is convenient for personnel to observe the inside of the culture chamber and the culture dish. The culture chamber is heated at a constant temperature, and the nutrient solution inside the culture dish contains water, which is easily evaporated into water vapor after heating. The water vapor will adhere to the cover and condense into water droplets. The soft layer is bonded to the bottom of the cover, so that the cover can be scraped by the soft layer during the rotation process, and the water vapor at the bottom of the cover is scraped clean, thereby ensuring the permeability of the cover and facilitating personnel to observe the situation inside the culture chamber.

[0017] Preferably, both sides of the crossbeam are provided with guide grooves, which are inclined downward from the column direction toward the shell side wall along the crossbeam side wall, and a water storage chamber is provided on the outer wall of the shell, and the lower end of the collection groove is connected to the water storage chamber. This prevents the soft layer on the top of the crossbeam from scraping off the water drops at the bottom of the cover plate and dripping down along the crossbeam onto the tray, thereby preventing the water drops from flowing into the groove and contacting the culture dish, causing a large change in the local temperature of the culture dish and affecting the growth of microorganisms. This ensures the stability of the microbial growth environment, thereby ensuring the efficiency and survival rate of microbial culture.

[0018] Preferably, the branch pipes are all inclined downward in a direction away from the axis of the extension pipe based on the axis of the extension pipe, so that the microorganisms and culture solution inside the branch pipe can flow back into the culture dish under the action of gravity after the rubber-tipped dropper has finished sampling, thereby avoiding a large amount of microorganisms and nutrient solution remaining inside the branch pipe, reducing the death of microorganisms inside the branch pipe, ensuring the survival rate of microorganisms, and ensuring the cleanliness of the branch pipe, thereby ensuring the accuracy of the next sampling.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The present invention is a microorganism culture integrated equipment, in which a cover is rotatably provided, and the cover seals the culture chamber so that the inside of the culture chamber is kept at a constant temperature. A sampling port is provided on the cover, and a rubber-tipped dropper is inserted into the culture chamber during sampling, and the sampling port is sealed by the rubber-tipped dropper to avoid temperature loss inside the culture chamber during sampling. The structure is simple, and the cover can be rotated to facilitate sampling of all culture dishes inside the culture chamber.

[0021] 2. The integrated microbial culture equipment designed by the present invention is also provided with a sampling assembly inside the culture chamber. A plurality of branch pipes are provided on the lower side of the extension tube of the sampling assembly. The plurality of branch pipes are evenly distributed on the branch pipes. When sampling the microorganisms, as many different positions in the culture dish as possible can be sampled, thereby improving the accuracy of sampling detection and making the taken samples more reflective of the overall situation of the microorganisms inside the culture dish.

[0022] 3. The integrated microbial culture equipment designed by the present invention is also provided with a first threaded portion and a second threaded portion. The first threaded portion and the second threaded portion cooperate with each other so that the extension tube can rotate when subjected to an axial force. When the extension tube rotates, the branch tube can be driven to rotate together and stir the microbial culture solution inside the culture dish, so as to further improve the accuracy of sampling and detection, and also allow the nutrient solution and microorganisms inside the culture dish to be evenly spread throughout the culture dish, allowing the microorganisms to grow and develop better. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0024] Figure 2 For the present invention Figure 1 The enlarged view of point A in the middle;

[0025] Figure 3 It is a front view of the present invention;

[0026] Figure 4 For the present invention Figure 3 Sectional view at the middle BB;

[0027] Figure 5 For the present invention Figure 4 Enlarged view of point C in the middle;

[0028] Figure 6 It is a schematic diagram of the three-dimensional structure of the sampling assembly in the present invention;

[0029] Figure 7 It is a front view of the sampling assembly of the present invention;

[0030] Figure 8 For the present invention Figure 7 Enlarged view of point D in the middle.

[0031] In the figure: 1. shell; 2. cover plate; 3. column; 4. culture chamber; 5. heating plate; 6. tray; 7. groove; 8. sampling port; 9. bracket; 10. sleeve; 11. extension tube; 12. branch pipe; 13. support platform; 14. spring groove; 15. reset spring; 16. first threaded portion; 17. second threaded portion; 18. stirring blade; 19. slot; 20. ball bearing; 21. locking spring; 22. locking groove; 23. crossbeam; 24. soft layer; 25. guide groove; 26. water storage chamber; 27. culture dish. DETAILED DESCRIPTION

[0032] See also Figures 1 to 8 The present invention provides an integrated microbial cultivation device, and the technical solution is as follows:

[0033] A microorganism culture integrated device comprises an outer shell 1 and a cover plate 2, wherein the outer shell 1 is cylindrical, a column 3 is coaxially fixedly installed in the middle of the outer shell 1, the cover plate 2 is made of a transparent material, the cover plate 2 is detachably rotatably installed on the column 3 for closing the opening of the outer shell 1, a culture chamber 4 is formed between the outer shell 1 and the cover plate 2, a heating plate 5 for heating the culture chamber 4 is arranged at the bottom of the outer shell 1, a circular tray 6 is coaxially fixedly installed on the column 3, the tray 6 is located above the heating plate 5 and does not contact the heating plate 5, six circular grooves 7 for storing culture dishes 27 are arranged on the tray 6, the six grooves 7 are evenly distributed on the tray 6 with the center of the tray 6 as the reference circumference, and the centers of the six grooves 7 are all located on the same circumference with the center of the tray 6 as the center, a sampling port 8 for inserting a rubber-tipped dropper is arranged on the cover plate 2, and the center of the sampling port 8 and the center of the groove 7 are located on the same circumference in the vertical direction.

[0034] refer to Figure 1 and Figure 2 A closing component for closing the sampling port 8 is provided on the shell 1, and the closing component includes a beam 23 fixedly installed inside the culture chamber 4, one end of the beam 23 is fixedly installed on the inner wall of the shell 1, and the other end is fixedly installed on the column 3, the beam 23 and the groove 7 are staggered in the vertical direction, and a soft layer 24 is provided on the top of the beam 23, and the soft layer 24 is in contact with the bottom of the cover plate 2.

[0035] refer to Figure 1 and Figure 2 A guide groove 25 is provided on both sides of the cross beam 23. The guide groove 25 is inclined downward from the column 3 toward the side wall of the shell 1 along the side wall of the cross beam 23. A water storage chamber 26 is provided on the outer wall of the shell 1, and the lower end of the collection tank is connected to the water storage chamber 26.

[0036] Figure 1 as well as Figures 4 to 8 , six sampling assemblies for auxiliary sampling are also provided on the tray 6, and the six sampling assemblies correspond to the six grooves 7 one by one. Each sampling assembly includes a bracket 9 arranged above each groove 7, and the bracket 9 is clamped on the tray 6, and the bracket 9 spans the corresponding groove 7. A cylindrical sliding sleeve 10 is fixed on the bracket 9, and the sliding sleeve 10 is coaxially arranged with the groove 7. The center of the sliding sleeve 10 is coaxially slidably installed with an extension tube 11, and the upper end of the extension tube 11 is used to dock with the rubber head dropper. The lower side of the extension tube 11 is evenly distributed with four branch tubes 12 on the circumference, and the four branch tubes 12 are evenly distributed on the outer wall of the extension tube 11. The four branch tubes 12 are connected to the extension tube 11, and each branch tube 12 is inclined downward in a direction away from the axis of the extension tube 11 based on the axis of the extension tube 11. A stirring blade 18 is also fixedly installed on each branch tube 12, and the stirring blade 18 is at an angle of 45° with the horizontal plane.

[0037] refer to Figure 1 , Figure 4 and Figure 5 A card slot 19 is provided on the sliding sleeve 10, and a ball 20 is arranged inside the card slot 19. A locking spring 21 is also arranged inside the card slot 19. The locking spring 21 is located between the bottom of the card slot 19 and the ball 20 and is used to push the ball 20 out of the card slot 19. The centers of all the balls 20 are located on the same horizontal circumference with the center of the tray 6 as the reference. A locking slot 22 matching the ball 20 is provided at the bottom of the cover plate 2, and the locking slot 22 is located on the same circumference as the ball 20 in the vertical direction.

[0038] refer to Figure 5 A support platform 13 is also provided at the upper end of the extension tube 11, a spring groove 14 is coaxially provided on the sliding sleeve 10, a return spring 15 is provided inside the spring groove 14, one end of the return spring 15 abuts against the bottom of the spring groove 14, and the other end abuts against the bottom of the support platform 13, the return spring 15 is used to keep the extension tube 11 pushed up, a first threaded portion 16 is provided on the extension tube 11, and a second threaded portion 17 that cooperates with the first threaded portion 16 is provided on the sliding sleeve 10. The extension tube 11 is meshed with the second threaded portion 17 on the sliding sleeve 10 through the first threaded portion 16, and the thread lead angles of the first threaded portion 16 and the second threaded portion 17 are both greater than the equivalent friction angle, that is, the meshing between the first threaded portion 16 and the second threaded portion 17 will not self-lock.

[0039] When using, refer to Figures 1 to 8 , lift the cover plate 2 upward along the axis direction of the column 3, and remove the cover plate 2 from the column 3. Then, remove the brackets 9 from the tray 6 one by one, then install the culture dish 27 filled with the microbial culture solution into the groove 7 on the tray 6, and finally reinstall the bracket 9 corresponding to the groove 7 on the tray 6. When reinstalling the bracket 9, note that the ball 20 on the top of the sleeve 10 needs to be on the same horizontal circumference based on the axis of the tray 6, and the radius of the circle where the ball 20 is located is equal to the distance from the center of the locking groove 22 to the center of the cover plate 2. Then, rotate the cover plate 2 back onto the column 3, and then start the heating plate 5. The heating plate 5 heats the culture chamber 4 at a constant temperature to provide a constant temperature environment for the growth of microorganisms. Then, the cover plate 2 is rotated so that the sampling port 8 on the cover plate 2 is aligned with the cross beam 23, the soft layer 24 on the top of the cross beam 23 is close to the bottom of the cover plate 2, and the sampling port 8 on the cover plate 2 is closed to prevent the temperature inside the culture chamber 4 from radiating from the sampling port 8 to the outside of the culture chamber 4.

[0040] In the process of microbial cultivation, reference Figure 1 , Figure 4 , Figure 5 and Figure 6When it is necessary to sample, test and observe the microorganisms inside the culture chamber 4, turn the cover plate 2 clockwise. When the sampling port 8 rotates to the top of the culture dish 27 along the rotation direction of the cover plate 2, slowly continue to rotate the cover plate 2 until a "click" sound is heard. At this time, the ball 20 is stuck in the locking groove 22 under the action of the locking spring 21, and the sampling port 8 is opened without the cover of the crossbeam 23, and the axis of the sampling port 8 coincides with the axis of the extension tube 11. At this time, stop rotating the cover plate 2, take out the rubber-tipped dropper used for sampling, hold the tube body of the rubber-tipped dropper with your left hand, and vertically insert the rubber-tipped dropper into the culture chamber 4 from the sampling port 8. The head of the rubber-tipped dropper abuts against the upper end of the extension tube 11 and squeezes the rubber-tipped dropper downward. Under the squeezing of the rubber-tipped dropper, the extension tube 11 moves vertically downward along the axis direction of the extension tube 11, and the reset spring 15 is compressed.

[0041] During the downward movement of the extension tube 11, Figure 4 , Figure 5 and Figure 6 , under the meshing guidance of the first threaded portion 16 and the second threaded portion 17, the extension tube 11 rotates and moves downward. The rotation of the extension tube 11 will also drive the branch tube 12 on the lower side of the extension tube 11 to rotate together, and the rotation of the branch tube 12 will stir the culture solution inside the culture dish 27, so that the microorganisms in the culture solution can be evenly distributed in the culture solution, allowing the microorganisms to better absorb the nutrients in the culture solution, and avoid the microorganisms in the culture solution from clumping and affecting growth. The stirring piece 18 on the branch tube 12 can better disturb the culture solution, allowing the culture solution to flow better in the vertical direction, and then make the distribution of microorganisms in the culture solution more even. When the extension tube 11 moves downward to the extreme position, the bottom of the extension tube 11 has not yet contacted the bottom of the culture dish 27. Then, pinch the rubber tip of the rubber dropper with your right hand and release it again. Under the action of negative pressure, the rubber dropper sucks the culture solution inside the culture dish 27 from the bottom of the extension tube 11 and the ports of the four branch pipes 12 into the extension tube 11, and continues to absorb the culture solution inside the extension tube 11 upward under the action of negative pressure, and finally enters the rubber dropper that abuts against the top of the extension tube 11. Finally, lift the tube body of the rubber dropper upward along the axis direction of the rubber dropper with your left hand, take the rubber dropper out of the sampling port 8, and then take the culture solution inside the culture dish 27 out of the culture chamber 4. And because the branch pipes 12 are distributed in a star shape on the extension tube 11, the sample sucked out by the rubber dropper can better reflect the overall growth of microorganisms inside the culture dish 27.

[0042] After the rubber-tipped dropper is taken out from the sampling port 8, refer to Figures 4 to 8After the extension tube 11 loses the squeezing force of the rubber dropper, the reset spring 15 resets upward under its own elastic force and drives the extension tube 11 to move upward. When the extension tube 11 moves upward, it rotates under the action of the first threaded portion 16 and the second threaded portion 17, and the branch pipe 12 on the extension tube 11 stirs the culture solution again, so that the microorganisms are evenly distributed in the culture solution.

[0043] refer to Figure 1 After taking out the sample of a culture dish 27 for inspection, continue to rotate the cover plate 2 clockwise to the top of the next culture dish 27. During the rotation of the cover plate 2, the ball 20 is squeezed. After being squeezed, the ball 20 will be retracted into the slot 19 without interfering with the movement of the cover plate 2. Then repeat the above operation to take out the sample of the culture dish 27 for inspection until the samples of all the culture dishes 27 in the culture chamber 4 are taken out for inspection. After all the sampling is completed, the cover plate 2 is rotated back to its original position to align the crossbeam 23 with the sampling port 8 again to avoid temperature leakage inside the culture chamber 4.

[0044] Among them, reference Figure 2 During the rotation of the cover plate 2, the soft layer 24 on the top of the beam 23 will scrape the bottom of the cover plate 2. If there is water vapor or water droplets on the bottom of the cover plate 2, they will be scraped off by the soft layer 24. Finally, the water droplets will flow downward along the side wall of the beam 23 into the guide groove 25, and finally flow to the inside of the water storage chamber 26 under the guidance of the guide groove 25 and be collected.

[0045] In addition, refer to Figures 1 to 8 When it is necessary to supplement the culture solution in the culture dish 27 with nutrients, the cover plate 2 is rotated in the above manner to align the sampling port 8 with the axis of the extension tube 11, and the nutrient solution is sucked with a rubber-tipped dropper, and then the rubber-tipped dropper is inserted into the culture chamber 4 from the sampling port 8, and the rubber-tipped dropper is docked with the top of the extension tube 11. Then, the rubber tip of the rubber-tipped dropper is squeezed to allow the nutrient solution in the rubber-tipped dropper to flow along the extension tube 11 into the culture dish 27, and then the extension tube 11 is pressed downward along the axis of the extension tube 11 with the rubber-tipped dropper. After the extension tube 11 is pressed, the branch tube 12 is driven to rotate, and the branch tube 12 and the stirring blade 18 on the branch tube 12 stir the culture solution in the culture dish 27 and evenly distribute the newly added nutrient solution in the culture solution, which helps the microorganisms to better absorb nutrients and thus improve the growth rate of the microorganisms. Finally, the rubber-tipped dropper is taken out from the sampling port 8 , and after the nutrient solution is added to all the culture dishes 27 , the sampling port 8 is rotated to be aligned with the crossbeam 23 , and the sampling port 8 is re-sealed.

[0046] A specific embodiment of the present invention is described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above described embodiment. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments without departing from the principles and ideas of the present invention should still fall within the scope of protection of the present invention.

Claims

1. A microorganism culture integrated device, comprising a shell (1) and a cover plate (2), wherein the shell (1) is cylindrical, a column (3) is coaxially fixedly installed in the middle of the shell (1), the cover plate (2) is detachably rotatably installed on the column (3), the cover plate (2) is used to close the opening of the shell (1), a culture chamber (4) is formed between the shell (1) and the cover plate (2), a heating plate (5) for heating the temperature of the culture chamber (4) is arranged at the bottom of the shell (1), and the characteristics are as follows: A circular tray (6) is coaxially fixedly mounted on the column (3), the tray (6) is located above the heating plate (5) and does not contact the heating plate (5), a plurality of circular grooves (7) for storing culture dishes (27) are provided on the tray (6), the plurality of grooves (7) are evenly distributed on the tray (6) with the center of the tray (6) as the reference circumference, a sampling port (8) for inserting a rubber-tipped dropper is provided on the cover plate (2), the center of the sampling port (8) and the center of the groove (7) are located on the same circumference in the vertical direction, a closing component for closing the sampling port (8) is provided on the housing (1), and a plurality of sampling components for auxiliary sampling are also provided on the tray (6), and the plurality of sampling components correspond one to one to the plurality of grooves (7).

2. The integrated microbial cultivation device according to claim 1, characterized in that: The sampling assembly comprises a bracket (9) arranged above each groove (7), the bracket (9) is clamped on the tray (6), the bracket (9) spans the corresponding groove (7), a cylindrical sliding sleeve (10) is fixed on the bracket (9), the sliding sleeve (10) is coaxially arranged with the groove (7), an extension tube (11) is coaxially slidably installed at the center of the sliding sleeve (10), the upper end of the extension tube (11) is used to connect with a rubber-tipped dropper, and the lower side of the extension tube (11) has a plurality of branch tubes (12) in a circumferential array, and the plurality of branch tubes (12) are connected to the extension tube (11).

3. The integrated microbial cultivation device according to claim 2, characterized in that: The upper end of the extension tube (11) is provided with a support platform (13), the sliding sleeve (10) is coaxially provided with a spring groove (14), a return spring (15) is provided inside the spring groove (14), one end of the return spring (15) abuts against the bottom of the spring groove (14), and the other end abuts against the bottom of the support platform (13), the return spring (15) is used to keep the extension tube (11) lifted upward, the extension tube (11) is provided with a first threaded portion (16), the sliding sleeve (10) is provided with a second threaded portion (17) that cooperates with the first threaded portion (16), the extension tube (11) is meshed with the second threaded portion (17) on the sliding sleeve (10) through the first threaded portion (16), and the thread lead angles of the first threaded portion (16) and the second threaded portion (17) are both greater than the equivalent friction angle.

4. The integrated microbial cultivation device according to claim 3, characterized in that: The branch pipes (12) are all fixedly mounted with stirring blades (18), and the stirring blades (18) are all at an angle of 30° to 60° with the horizontal plane.

5. The integrated microbial cultivation device according to claim 2, characterized in that: The sliding sleeve (10) is provided with a slot (19), and a ball (20) is arranged inside the slot (19). A locking spring (21) is also arranged inside the slot (19). The locking spring (21) is located between the bottom of the slot (19) and the ball (20) and is used to push the ball (20) out of the slot (19). The centers of all the balls (20) are located on the same horizontal circumference with the center of the tray (6) as a reference. The bottom of the cover plate (2) is provided with a locking slot (22) that matches the ball (20). The locking slot (22) is located on the same circumference as the ball (20) in the vertical direction. When the ball (20) is inserted into the locking slot (22), the sampling port (8) is aligned with the extension tube (11) on the sliding sleeve (10) where the ball (20) is located.

6. The integrated microbial cultivation device according to claim 1, characterized in that: The sealing component comprises a crossbeam (23) fixedly mounted inside the culture chamber (4), one end of the crossbeam (23) being fixedly mounted on the inner wall of the outer shell (1), and the other end being fixedly mounted on the column (3), the crossbeam (23) being staggered with the groove (7) in the vertical direction, the crossbeam (23) being in contact with the bottom of the cover plate (2), and when the sampling port (8) is located directly above the crossbeam (23), the crossbeam (23) seals the sampling port (8).

7. The integrated microbial cultivation device according to claim 6, characterized in that: The cover plate (2) is made of a transparent material, a soft layer (24) is provided on the top of the cross beam (23), and the soft layer (24) is in contact with the bottom of the cover plate (2).

8. The integrated microbial cultivation device according to claim 1, characterized in that: Both sides of the cross beam (23) are provided with guide grooves (25), and the guide grooves (25) are inclined downward from the direction of the column (3) toward the side wall of the shell (1) along the side wall of the cross beam (23). A water storage chamber (26) is provided on the outer side wall of the shell (1), and the lower end of the collection groove is connected to the water storage chamber (26).

9. The integrated microbial cultivation device according to claim 2, characterized in that: Each branch pipe (12) is inclined downward in a direction away from the axis of the extension pipe (11) based on the axis of the extension pipe (11) where it is located.

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