Phytobacterium plantarum KSFY202407 capable of resisting fatigue and improving athletic ability and application of phytobacterium plantarum KSFY202407

Abstract

The invention provides a plant lactobacillus KSFY202407 capable of resisting fatigue and improving athletic ability and application of the plant lactobacillus KSFY202407, and belongs to the technical field of microorganisms. The preservation number of the plant lactobacillus KSFY202407 provided by the invention is CGMCC No.31104. The strain can maintain stable increase of mouse weight, prolong exhaustive running and exhaustive swimming time, reduce blood lactic acid concentration after excessive exercise, improve the content of muscle glycogen and hepatic glycogen in gastrocnemius muscle, reduce serum creatine kinase and blood urea nitrogen concentration, and improve the immunity of mice. The obvious effect of up-regulating the mRNA expression levels of AMPK, PGC1-alpha, SOD2 and GPx1 is achieved. It is shown that the KSFY202407 can improve exercise tolerance, relieve exercise-induced fatigue and promote exercise ability, and a candidate strain is provided for development of exercise-related products.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to Lactobacillus plantarum KSFY202407 for resisting fatigue and improving exercise ability and applications thereof. Background Art

[0002] For a long time, regular and scientific physical activities have been regarded not only as an effective means to prevent different chronic diseases and related complications, but also as a treatment method. In particular, aerobic exercise has been shown to significantly improve physiological adaptability by enhancing cardiovascular capacity and energy metabolites. However, with the acceleration of the pace of life and work, people are often in a state of continuous or high-intensity work and exercise. Energy consumption makes people prone to fatigue, and continuous fatigue in turn prompts people to reduce exercise, which leads to a decline in people's physical fitness and a continuous sub-healthy state of fatigue. In addition, intense or excessive exercise can cause cell and muscle damage, break the balance between oxidative stress and antioxidant systems in the body, lead to the generation of sports fatigue, and have a negative impact on the physical health and career of professional athletes. Therefore, it is of great significance to find effective means of intervention to improve physical fitness, enhance athletic ability, and improve sports fatigue.

[0003] In recent years, the number of studies on probiotics in the field of sports and the types of nutritional supplements containing probiotics have increased significantly. Current literature shows that probiotics that have a positive effect on improving athletic ability mainly include Lactobacillus plantarum, Lactobacillus casei, Lactobacillus acidophilus, Bifidobacterium and Streptococcus thermophilus. At the same time, probiotics have a positive promoting effect on human gastrointestinal function and immune regulation. In addition, animal experiments have confirmed that supplementing with specific probiotics can increase the endurance running time of mice to a certain extent, which provides inspiration for improving human sports endurance performance by supplementing probiotics. The Kashgar area of ​​Xinjiang Uygur Autonomous Region in my country is an area where ethnic minorities live. The integration of multi-ethnic dietary habits makes the local naturally fermented yogurt rich in nutritional value and good health care effects, and the fermented microorganisms in yogurt also have strong resistance and good diversity, which is of great development and utilization value. Therefore, it is of great significance to develop probiotics in the field of sports in traditional fermented products. Summary of the invention

[0004] In view of this, the purpose of the present invention is to provide an anti-fatigue, sports ability-enhancing Lactobacillus plantarum KSFY202407 and its application. The Lactobacillus plantarum KSFY202407 provided by the present invention can improve sports endurance, reduce sports fatigue, promote sports ability, and provide a candidate strain for the development of sports-related products.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: A plant lactobacillus KSFY202407 for resisting fatigue and improving exercise ability, wherein the plant lactobacillus KSFY202407 is classified and named Lactiplantibacillus plantarum The deposit number is CGMCC No.31104, and it was deposited in the General Microbiology Center of China Microbiological Culture Collection Administration on June 27, 2024. The deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0006] The present invention also provides a microbial agent, comprising the Lactobacillus plantarum KSFY202407 described in the above technical solution.

[0007] The present invention also provides an application of the Lactobacillus plantarum KSFY202407 described in the above technical solution or the microbial agent described in the above technical solution in the preparation of an anti-fatigue product.

[0008] The present invention also provides an application of the Lactobacillus plantarum KSFY202407 described in the above technical solution or the microbial agent described in the above technical solution in the preparation of a product for improving sports endurance.

[0009] The present invention also provides an application of the Lactobacillus plantarum KSFY202407 described in the above technical solution or the microbial agent described in the above technical solution in the preparation of a product for improving athletic ability.

[0010] In the present invention, the products preferably include but are not limited to food, health products, and medicines.

[0011] In the present invention, the content of Lactobacillus plantarum KSFY202407 in the product is preferably not less than 1×10 8 CFU / kg.

[0012] Beneficial technical effect: The present invention provides a plant lactobacillus KSFY202407 for resisting fatigue and improving exercise ability and its application. The classification of the plant lactobacillus KSFY202407 is named Lactiplantibacillus plantarum , with the deposit number of CGMCC No.31104, deposited in the General Microbiology Center of China Microbiological Culture Collection Administration on June 27, 2024, and the deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. This strain can maintain the stable weight growth of mice, prolong the exhaustive running and exhaustive swimming time, reduce the blood lactate concentration after excessive exercise, increase the content of muscle glycogen and liver glycogen in gastrocnemius muscle, reduce serum creatine kinase and blood urea nitrogen concentration, and upregulate AMPK , PGC1-α , SOD2 and GPx1The significant effect on mRNA expression level indicates that KSFY202407 can improve exercise endurance, reduce exercise fatigue, promote exercise ability, and provide a candidate strain for the development of sports-related products. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 The effect of KSFY202407 on the body weight of mice; different lowercase letters indicate significant differences ( P ﹤0.05), the same below; Figure 2 The effect of KSFY202407 on exhaustive running and exhaustive swimming time in mice; Figure 2 The left picture shows running to exhaustion, and the right picture shows swimming to exhaustion; Figure 3 The effect of KSFY202407 on the changes of blood lactate concentration after excessive exercise in mice; Figure 4 The effect of KSFY202407 on gastrocnemius muscle fibers in mice; Figure 5 The effect of KSFY202407 on MG and HG in gastrocnemius muscle, serum CK and BUN concentration in mice; Figure 6 KSFY202407 for the gastrocnemius muscle of mice AMPK , PGC1-α , SOD2 and GPx1 The effect of mRNA expression level. DETAILED DESCRIPTION

[0014] In order to better understand the present invention, the content of the present invention is further explained in conjunction with the following examples, but the content of the present invention is not limited to the following examples. The materials, reagents, etc. used in the examples and test examples of the present invention, unless otherwise specified, can be obtained from commercial channels; the methods used in the examples and test examples of the present invention, unless otherwise specified, are conventional methods.

[0015] Example 1 Strain Isolation and Identification 1.1 Materials Naturally fermented yogurt was collected from Kashgar, Xinjiang Uygur Autonomous Region. 40 mL of yogurt was placed in a sterile centrifuge tube, placed in a food sampling box, and stored in a 4°C refrigerator in the laboratory for later use.

[0016] 1.2 Isolation and purification of strains Take 1 mL of yogurt sample and dilute it 10 times with sterile saline to 10 -6 , then take 10 -4 , 10 -5 , 10 -6100 μL of the bacterial solution of 3 gradients was spread on MRS medium and cultured at 37℃ for 24h~48h, and the colony morphology was observed and recorded. The colonies with different morphologies on the plate were picked and streaked on MRS medium. After culturing at 37℃ for 48h, the single colonies with different morphologies on the plate were picked and streaked on MRS medium again. This was repeated 2 to 3 times until a pure single colony with consistent morphology was obtained, which was numbered KSFY202407.

[0017] 1.3 Identification of strains The purified suspected target strain was inoculated into MRS broth and cultured at 37°C for 18h~24h. DNA was extracted using a bacterial genomic DNA extraction kit (item number: K0512, purchased from Thermo Fisher Scientific, USA). The extracted DNA was numbered and stored in a -20°C freezer for later use.

[0018] The extracted DNA was subjected to PCR amplification. The amplification system was as follows: 1 μL of upstream primer 27F (5'-AGA GTT TGA TCCTGGCTC AG-3', SEQ ID NO.1), 1 μL of downstream primer 1495R (5'-CTA CGG CTA CCTTGT TAC GA-3', SEQ ID NO.2), 12.5 μL of 2× Taq plus Buffer, 1 μL of template DNA, and sterile ddH 2 O to make up the system to 25 μL. Sterile ultrapure water was used instead of template DNA as a negative control. Amplification conditions were: 94°C for 5 min; 94°C for 30 s, 55°C for 30 s, 72°C for 1 min, 29 cycles, and 72°C for 5 min.

[0019] Take 5 μL of amplified product for agarose gel electrophoresis detection, the agarose concentration is 1.5%, the electrophoresis conditions are 110V, 45min. The successfully detected PCR product was sent to Beijing Qingke Biotechnology Co., Ltd. for sequencing, and the sequenced sequence was compared and analyzed using the BLAST (Basic Local Alignment Search Tool) program in NCBI.

[0020] The strain numbered KSFY202407 was isolated and purified from a naturally fermented yogurt sample in Kashgar, Xinjiang Uygur Autonomous Region. 16S rDNA species analysis showed that the strain was Lactobacillus plantarum. It was deposited in the General Microbiology Center of China Culture Collection on June 27, 2024, with the deposit number CGMCC No.31104. The deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0021] The nucleotide sequence of 16S rDNA of KSFY202407 strain is shown in SEQ ID NO.3:

[0022] Example 2 2.1 Materials and Equipment As shown in Table 1 and Table 2.

[0023] Table 1 Experimental materials and reagents Table 2 Instruments and equipment 2.2 Experimental methods 2.2.1 Animal Experiment Design Forty 6-week-old (27±1g) SPF male ICR mice were purchased from Hunan Slake Jingda Experimental Animal Co., Ltd. (Animal Qualification License No.: SCXK (Xiang) 2019-0004) and kept in a constant temperature and humidity environment with a temperature of 25±2℃ and a humidity of 50±5%. The mice were allowed to eat and drink freely. All animal experiments were carried out in strict accordance with the laboratory animal use regulations and animal ethics experimental requirements. After 7 days of adaptive feeding, the mice were randomly divided into 4 groups, namely: quiet control group, exercise control group, KSFY202407 quiet group and KSFY202407 exercise group. The whole experiment lasted for 7 weeks, of which the 1st to 6th weeks were the running exercise training period and the 7th week was the behavioral test period. During the experimental period, the quiet control group and the exercise control group were gavaged with 0.2mL of sterile saline at the same time every day, and the KSFY202407 quiet group and KSFY202407 exercise group were gavaged with 0.2mL of 1×10 8 CFU / kg of KSFY202407 bacterial suspension. The exercise control group and the KSFY202407 exercise group underwent 6 weeks of running exercise training. Before the formal training, they ran at a speed of 12m / min for 25min for 1 day to adapt. In the first week, they started at a speed of 12m / min, and the speed increased by 2m / min every week. From the third week, the slope of the treadmill increased by 5°, and the slope was maintained at 10° from the fourth to the sixth week. The daily running time was 35min. In the seventh week, all mice underwent exhaustive swimming test, exhaustive running test and blood lactate index determination.

[0024] 2.2.2 Exhaustive swimming test in mice Before the exhaustive swimming test, mice were subjected to 10 minutes of weighted swimming adaptation training (water temperature 28±1℃, water depth about 40cm), with a weight of about 5% of the mouse's body weight placed at the base of the mouse's tail. Immediately after the end, the mouse's fur was dried with a towel and blown with hot air until completely dry. A formal exhaustive swimming experiment was conducted on the second day after the weighted swimming adaptation training. The experimental conditions were the same as those of the adaptive training experiment. During the experiment, the mouse's limbs were kept in continuous motion. The exhaustion criterion was that the mouse's nostrils were completely submerged in the water for 7 seconds and could not float to the surface. The time taken for the mouse to swim to exhaustion was recorded.

[0025] 2.2.3 Exhaustive running experiment in mice The mice were subjected to exhaustion running test using an 8-channel experimental mouse treadmill. The slope was set to 10°, and the treadmill track speed was gradually increased (10 m / min for 5 min, 16 m / min for 10 min, 21 m / min for 10 min, 26 m / min for 10 min, 31 m / min for 10 min, 36 m / min for 10 min, 41 m / min for 10 min, 46 m / min for 10 min) until the mice were exhausted. The exhaustion criterion for mice was that the number of electric shocks reached 10 times, or they could not continue to run forward for 10 seconds. The time taken for the mice to run to exhaustion was recorded.

[0026] 2.2.4 Determination of blood lactate concentration in mice The lactic acid values ​​of mice were measured at rest before exhaustive running, 5 minutes after exhaustion, and 30 minutes after exhaustion. The blood was collected from the tip of the mouse's tail using a blood collection needle that came with the lactate detector. The first drop of blood was wiped off, and the whole blood was drawn with a test strip to read the data on the detector. Before and after each blood test, the blood collection site on the mouse's tail was disinfected with a cotton ball dipped in iodine.

[0027] 2.2.5 Mouse dissection and sampling After all behavioral tests were completed, the mice were fasted but not watered for 12 hours, and then all mice were euthanized and biological samples were collected. The whole blood of the mice was collected and placed at room temperature for 30 minutes before centrifugation at 4000r / min for 15 minutes at 4°C. The upper serum was separated and aspirated and stored at -80°C for later use.

[0028] 2.2.6 H&E staining analysis of mouse gastrocnemius muscle The gastrocnemius muscle of mice was fixed in paraformaldehyde tissue fixative. After the tissue samples were fixed and dehydrated, they were embedded in paraffin. The paraffin-embedded blocks were cut into 4 μm thin slices by a microtome, and then routinely dewaxed and hydrated with gradient ethanol. Subsequently, they were stained with hematoxylin for 2 minutes, differentiated with 1% hydrochloric acid ethanol, and blued with dilute ammonia water. Finally, they were stained with eosin for 15 seconds, dehydrated with gradient ethanol, and sealed after dehydration. The histological changes of the gastrocnemius muscle of mice were observed and analyzed under an electron microscope.

[0029] 2.2.7 Determination of MG and HG in gastrocnemius muscle, serum CK and BUN concentrations in mice According to the instructions of the enzyme linked immunosorbent assay (ELISA) kit, the concentrations of gastrocnemius muscle glycogen (MG), hepatic glycogen (HG) and serum creatine kinase (CK) were measured. At the same time, the concentration of blood urea nitrogen (BUN) was measured according to the instructions of the mouse urea nitrogen detection kit.

[0030] 2.2.8 Mouse gastrocnemius muscle AMPK / PGC1-α Determination of mRNA expression levels of pathway-related factors Weigh an appropriate amount of mouse gastrocnemius muscle tissue, add TRIzol for homogenization to extract RNA from the mouse tissue, and then use a spectrophotometer to measure the concentration and purity of the extracted total RNA, and reverse transcribe the total RNA into cDNA according to the instructions of the reverse transcription kit. After reverse transcription, prepare the cDNA reaction system in an eight-tube strip, namely 10μL of Master mix, 1μL of upstream and downstream primers, 1μL of template cDNA, and 7μL of sterile enzyme-free water. The prepared reaction system is amplified and detected using real-time fluorescence quantitative PCR (Quantitative Real-time PCR, qPCR) technology. Amplification conditions: pre-denaturation at 95℃ for 5min, then denaturation at 95℃ for 10s, annealing at 60℃ for 30s, and the whole process is repeated 40 times. After the determination, GAPDH As the internal reference gene, the formula Determine the relative expression of the target gene. The primer sequences used in this experiment are shown in Table 3.

[0031] Table 3 Primer sequences 2.2.9 Data processing and statistical analysis All data were expressed as mean ± standard deviation (SD) and analyzed using IBM SPSS 23.0 statistical software. One-way analysis of variance (ANOVA) and Duncan's multiple range test were used for comparison between groups. The same lowercase letters in ae indicate no significant difference between the two groups, and different lowercase letters indicate significant difference between the two groups P <0.05.

[0032] 2.3 Results and Analysis 2.3.1 Effect of KSFY202407 on the body weight of mice To evaluate the effects of long-term exercise training and KSFY202407 on the growth status of mice, the body weight of mice was measured and recorded once a week. Figure 1As shown in the figure, after the first week of adaptation, there was no significant difference in the weight of mice in each group. Throughout the experimental period, the weight of mice in each group showed a steady upward trend without abnormal fluctuations. At the end of the 7th week of the experiment, the weight of mice in the exercise control group, KSFY202407 quiet group and KSFY202407 exercise group was significantly different from that of the quiet control group ( P <0.05). Among them, the mice in the quiet control group weighed the most (41.80±0.85g), and the mice in the KSFY202407 exercise group weighed the lightest (36.51±0.71g). There was no significant difference between the exercise control group and the KSFY202407 quiet group ( P >0.05, but there was a significant difference between the KSFY202407 exercise group ( P <0.05).

[0033] 2.3.2 Effect of KSFY202407 on exhaustive exercise time in mice Exhaustive exercise is a common indicator for evaluating the exercise endurance of mice, including exhaustive running and exhaustive swimming. Figure 2 As shown in the left figure, the time taken by mice in each experimental group to reach exhaustion in the exhaustion running test was significantly different ( P <0.05), the overall trend showed that the mice in the quiet control group took the shortest time to reach exhaustion, followed by the exercise control group, KSFY202407 quiet group, and KSFY202407 exercise group. It is worth mentioning that not only the time to exhaustion of the KSFY202407 exercise group was significantly higher than that of the exercise control group, but also the time to exhaustion of the KSFY202407 quiet group was significantly longer than that of the exercise control group ( P <0.05). At the same time, the exhaustive swimming test was also used to detect changes in the exercise endurance of each group of mice, and the experimental results were consistent with those of the exhaustive running test ( Figure 2 Right), the mice in the KSFY202407 exercise group took the longest time to reach exhaustion, followed by the KSFY202407 quiet group, the exercise control group, and the quiet control group. There were significant differences in the exhaustion time between the groups ( P <0.05). The experimental results showed that KSFY202407 could significantly improve the exercise endurance of mice without exercise training, and could more effectively promote the exercise endurance of mice with daily exercise training.

[0034] 2.3.3 Effect of KSFY202407 on changes in blood lactate concentration after excessive exercise in mice Lactic acid is an important indicator for evaluating fatigue and aerobic metabolism after exercise. Figure 3 As shown, at rest time before exhaustive exercise, there was no significant difference in blood lactate concentration among the mice in each group ( P>0.05), and the overall level was low. However, 5 minutes after exhaustive exercise, the blood lactate content of mice in each group increased, and the differences between the groups were significant ( P <0.05). The blood lactate concentration of mice in the quiet control group was the highest among all groups, followed by the exercise control group, KSFY202407 quiet group, and KSFY202407 exercise group. After 30 minutes of exhaustive exercise, the blood lactate concentration of mice in each group decreased, and there were significant differences between the groups ( P <0.05). The KSFY202407 exercise group had the lowest blood lactate level, followed by the KSFY202407 quiet group and the exercise control group. The quiet control group had the highest blood lactate level. The results showed that regular daily exercise training can help accelerate the conversion of blood lactate after excessive exercise and reduce the accumulation of blood lactate. The intake of KSFY202407 can not only effectively alleviate the increase in blood lactate concentration after excessive exercise in mice, but also accelerate the conversion of blood lactate, reduce blood lactate concentration, and improve exercise fatigue.

[0035] 2.3.4 Effects of KSFY202407 on the histological structure of gastrocnemius muscle in mice from Figure 4 It can be observed that there were no pathological changes such as injury, edema, inflammation, hemorrhage, and necrosis in the gastrocnemius tissue of mice in each group, indicating that the 6-week aerobic exercise training was moderate and did not cause muscle tissue damage due to excessive exercise, and it can be used as an effective experimental subject to study the exercise ability of mice. Among them, the gastrocnemius muscle fibers in the quiet control group were mostly type IIB muscle fibers (white muscle fibers, thick fiber cross-section, light staining), and only a very small number of type I muscle fibers (red muscle fibers, thin fiber cross-section, darker staining) and type IIA muscle fibers (intermediate muscle fibers, thick fiber cross-section, darker staining) were visible. Compared with the quiet control group, the content of type I and type IIA muscle fibers in the exercise control group increased, and the number of these two types of muscle fibers was significantly increased in the KSFY202407 quiet group, while the gastrocnemius in the KSFY202407 exercise group contained more type I and type IIA muscle fibers. The experimental results show that long-term exercise training helps to increase the number of type I and type IIA muscle fibers related to endurance. Daily intake of KSFY202407 can not only increase the number of endurance-related muscle fibers in the gastrocnemius muscle of non-exercise individuals, but also significantly increase the exercise tolerance of the gastrocnemius muscle in combination with exercise training.

[0036] 2.3.5 Effects of KSFY202407 on MG and HG in gastrocnemius muscle, serum CK and BUN in mice Muscle glycogen and liver glycogen are the main energy sources for muscle metabolism, and their high level of reserves can effectively reduce muscle fatigue symptoms. Figure 5As shown in A and 5B, the contents of MG and HG in the gastrocnemius muscle of mice in the quiet control group were the lowest, followed by the exercise control group. Compared with the quiet control group, the contents of MG and HG in the gastrocnemius muscle of the KSFY202407 quiet group and the KSFY202407 exercise group were significantly increased ( P <0.05). Figure 5 C and 5D show the concentrations of serum CK and BUN in each group of mice, respectively. The results show that the quiet control group showed the highest serum CK and BUN content. In contrast, the CK and BUN content of the other three groups were significantly reduced, and the difference was significant. The lowest concentration was in the KSFY202407 exercise group, and the serum CK and BUN content of the KSFY202407 quiet group was also significantly lower than that of the exercise control group. The experimental results show that a six-week aerobic exercise training can significantly reduce the increase in serum CK and BUN concentrations after exhaustive exercise. The intake of KSFY202407 alone can also effectively reduce serum CK and BUN concentrations. KSFY202407 combined with aerobic exercise training can achieve better results.

[0037] 2.3.6 Effect of KSFY202407 on mouse gastrocnemius muscle AMPK, PGC1-α, SOD2 and GPx1 Effects of mRNA expression levels In order to further analyze the effect of KSFY202407 on improving sports fatigue and improving sports ability, AMPK, PGC1-α, SOD2 and GPx1 The mRNA expression level of Figure 6 Compared with the quiet control group without exercise training, the exercise control group and KSFY202407 and exercise group mice that underwent 6 weeks of aerobic exercise training had AMPK, PGC1-α, SOD2 and GPx1 The mRNA expression level of P <0.05), and the KSFY202407 quiet group mice also showed significantly higher AMPK, PGC1-α, SOD2 and GPx1 In this study, the mice in the exercise control group expressed higher levels of mRNA than those in the rest control group. SOD2 and GPx1 , proving that daily aerobic exercise training helps increase the levels of antioxidant enzymes. However, mice that only consumed KSFY202407 without exercise training showed higher levels of antioxidant enzymes than the exercise control group. SOD2 and GPx1, indicating that KSFY202407 has a significant effect on increasing the antioxidant enzymes in mice. In summary, daily exercise training can improve physical fitness, improve the reduced energy metabolism caused by excessive exercise, and alleviate the symptoms of oxidative stress after excessive exercise. The daily intake of KSFY202407 can also achieve the effect of daily exercise training. Daily exercise training combined with KSFY202407 intake can effectively increase exercise endurance and improve exercise ability.

[0038] 2.4 Conclusion KSFY202407 showed significant effects in maintaining stable weight growth of mice, prolonging exhaustive running and exhaustive swimming time, reducing blood lactate concentration after excessive exercise, increasing muscle glycogen and liver glycogen content in gastrocnemius muscle, reducing serum creatine kinase and blood urea nitrogen concentrations, and upregulating AMPK, PGC1-α, SOD2 and GPx1 mRNA expression levels. This indicates that Lactobacillus plantarum KSFY202407 can promote exercise capacity by improving exercise endurance and reducing exercise fatigue, providing a candidate strain for the development of sports-related nutritional supplements.

[0039] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Lactobacillus plantarum KSFY202407 for resisting fatigue and improving exercise ability, characterized in that: The classification of the plant lactobacillus KSFY202407 is named Lactiplantibacillus plantarum The deposit number is CGMCCNo.31104, and it was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on June 27, 2024. The deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

2. A microbial agent, characterized in that: The invention comprises the Lactobacillus plantarum KSFY202407 according to claim 1.

3. Use of the plant lactobacillus KSFY202407 according to claim 1 or the microbial agent according to claim 2 in the preparation of anti-fatigue products.

4. Use of the Lactobacillus plantarum KSFY202407 according to claim 1 or the microbial agent according to claim 2 in the preparation of a product for improving sports endurance.

5. Use of the Lactobacillus plantarum KSFY202407 according to claim 1 or the microbial agent according to claim 2 in preparing a product for improving athletic ability.

6. The use according to any one of claims 3 to 5, characterized in that: The products include but are not limited to food, health products, and medicines.

7. The use according to any one of claims 3 to 5, characterized in that: The content of Lactobacillus plantarum KSFY202407 in the product is not less than 1×10 8 CFU / kg.

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