Seed soaking agent and application thereof in promoting early maturing of oilseed rape and increasing yield of oilseed rape

Abstract

The invention discloses a seed soaking agent and application thereof in promoting early maturing of rape and increasing the yield of the rape. The seed soaking agent provided by the invention contains kaurenoic acid, zearalenone, phosphatidyl ethanolamine and dimethyl allyl triammonium diphosphate. Before the rape seeds are sown, the whole growth period of the rape can be effectively shortened by soaking the rape seeds with the seed soaking agent, early maturing of the rape is achieved, and the requirement for planting the rape in a temperature and light resource tight balance area and a constraint area is met. In addition, by utilizing the seed soaking agent, the seedling rate and the effective plant number of the oilseed rape can be effectively increased, and the yield of the oilseed rape is increased. Based on the seed soaking agent, the invention also provides a method for promoting the early maturing of the rape and / or increasing the yield of the rape, and compared with a method for promoting the early maturing of the rape through a cultivation means, the method for promoting the early maturing of the rape is simple to operate and does not need to consume excessive labor cost. The oilseed rape planting area can be enlarged, and the oilseed rape yield is increased.

Description

Technical Field

[0001] The invention belongs to the technical field of crop cultivation, and more specifically, relates to a seed soaking agent and its application in promoting early maturity of rapeseed and increasing rapeseed yield. Background Art

[0002] At present, my country's edible vegetable oil imports are as high as 70%, and the import sources are highly concentrated, and the supply security faces huge risks. Rapeseed (Brassica napus L.) is my country's largest oil crop. The Yangtze River Basin and the southern double-season rice areas are rich in light and temperature resources. Planting short-growing rapeseed during the winter idle period of rice fields and developing a "rice-rice-oil" three-crop production model are important ways to increase grain production, expand oil production and ensure supply on the basis of ensuring grain production.

[0003] The growth period of existing rapeseed varieties can basically meet the requirements of areas with loose temperature and light resources, but in areas with tight temperature and light resource balance and constraint areas, the growth period of rapeseed is still relatively long. In addition, the application of large-scale double-season rice high-yield cultivation technology has generally extended the growth period of rice, further compressing the growth period of rapeseed. Therefore, it is urgent to develop new technologies for promoting early maturity of rapeseed during the winter idle period of rice fields.

[0004] At present, the methods for promoting the early maturity of rapeseed are mostly by improving the cultivation means or combining the ripening agent. The operation of such methods is complicated and requires more time and energy. In contrast, the growth regulators that promote plant germination, growth and flowering and fruiting, such as gibberellins, are applied alone to promote the early maturity of rapeseed, which has the advantages of simple operation and easy operation, but the effect of gibberellins in shortening the whole growth period of rapeseed is still not ideal, and it is not possible to well meet the needs of planting rapeseed in the tight balance area of ​​temperature and light resources and the constraint area. In addition, how to ensure or further improve the yield of rapeseed while shortening the whole growth period of rapeseed is also a problem that needs attention. That is, a product that is easy to use and can promote the early maturity of rapeseed and increase its yield is now needed. Summary of the invention

[0005] In view of the deficiencies in the above-mentioned prior art, the present invention provides a seed soaking agent, which can improve the seedling rate of rapeseed, promote early maturity of rapeseed and increase its yield, and meet the needs of planting rapeseed in areas with tight balance of temperature and light resources and constrained areas.

[0006] The first object of the present invention is to provide a seed soaking agent.

[0007] The second object of the present invention is to provide the use of the seed soaking agent in improving the rapeseed seedling rate or in preparing a product for improving the rapeseed seedling rate.

[0008] The third object of the present invention is to provide the use of the seed soaking agent in increasing the effective number of rapeseed plants or in preparing a product for increasing the effective number of rapeseed plants.

[0009] The fourth object of the present invention is to provide the use of the seed soaking agent in shortening the whole growth period of rapeseed or in preparing a product for shortening the whole growth period of rapeseed.

[0010] The fifth object of the present invention is to provide the use of the seed soaking agent in increasing rapeseed yield or in preparing a product for increasing rapeseed yield.

[0011] A sixth object of the present invention is to provide a method for promoting early maturity of rapeseed and / or increasing rapeseed yield.

[0012] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0013] The present invention obtains a seed soaking agent that can promote the early maturity of rapeseed and increase its yield through continuous testing and adjustment, wherein the agent contains kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diphosphate triammonium salt. The seed soaking agent of the present invention and water are prepared into a solution in a certain proportion, and the rapeseed seeds are soaked in the prepared solution before germination and sowing, which can effectively shorten the full growth period of rapeseed (the number of days required for the crop from sowing to maturity) and achieve the early maturity of rapeseed. In addition, the seed soaking agent of the present invention can also effectively improve the seedling rate and effective plant number of rapeseed, thereby increasing the yield of rapeseed. Therefore, the present invention claims protection for the seed soaking agent.

[0014] The invention provides a seed soaking agent, which contains kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diphosphate triammonium salt.

[0015] Specifically, the seed soaking agent consists of kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diphosphate triammonium salt.

[0016] Specifically, in the seed soaking agent, the weight percentage of kaurenic acid is 27.5% to 29.7%, the weight percentage of zearalenone is 24.4% to 25.1%, the weight percentage of phosphatidylethanolamine is 23.6% to 24.3%, and the weight percentage of dimethylallyl diphosphate triammonium salt is 22.3% to 23.1%.

[0017] Preferably, in the seed soaking agent, the weight percentage of kaurenic acid is 28.7%; the weight percentage of zearalenone is 24.7%; the weight percentage of phosphatidylethanolamine is 24.0%; and the weight percentage of dimethylallyl diammonium phosphate is 22.6%; or in the seed soaking agent, the weight percentage of kaurenic acid is 27.5%; the weight percentage of zearalenone is 25.1%; the weight percentage of phosphatidylethanolamine is 24.3%; and the weight percentage of dimethylallyl diammonium phosphate is 23.1%.

[0018] When rapeseed seeds are soaked in this ratio of seed soaking agent and then sown, the data on yield components such as the number of siliques per plant of rapeseed are relatively higher, and the overall effect is better.

[0019] The use of the seed soaking agent of the present invention can not only shorten the whole growth period of rapeseed, but also effectively increase the seedling rate and effective number of rapeseed, thereby increasing the yield of rapeseed. Therefore, the present invention also requests the following applications of the seed soaking agent:

[0020] The present invention claims to protect the use of the seed soaking agent in improving the rapeseed seedling rate or in preparing a product for improving the rapeseed seedling rate.

[0021] The present invention also claims to protect the use of the seed soaking agent in increasing the effective number of rapeseed plants or in preparing a product for increasing the effective number of rapeseed plants.

[0022] The present invention also claims to protect the use of the seed soaking agent in shortening the whole growth period of rapeseed or in preparing a product for shortening the whole growth period of rapeseed.

[0023] The present invention also claims to protect the use of the seed soaking agent in increasing rapeseed yield or in preparing a product for increasing rapeseed yield.

[0024] In a specific embodiment of the present invention, the variety of rapeseed (Brassica napus L.) is Qingza 4.

[0025] Based on the seed soaking agent of the present invention, the present invention also provides a method for promoting early maturity of rapeseed and / or increasing rapeseed yield, the method comprising: preparing a solution with the seed soaking agent of the present invention and water in a weight ratio of 1:75 to 85, soaking rapeseed seeds in the prepared solution for 8 to 12 hours, and then germinating and sowing.

[0026] Specifically, after soaking the rapeseed seeds for 8 to 12 hours, wash them with water and then soak them to germinate until they turn white, dry the moisture on the surface of the seeds that turn white, place them at -4°C to -1°C for 12 to 16 days, and then carry out normal rapeseed sowing and cultivation management.

[0027] Specifically, the seed soaking agent and water are prepared into a solution in a weight ratio of 1:80, and the rapeseed seeds are soaked in the prepared solution for 10 hours before germination and sowing.

[0028] Specifically, 1 gram of the solution prepared by the seed soaking agent can soak 45 to 55 grams of rapeseed seeds.

[0029] More specifically, 1 g of the solution prepared with the seed soaking agent can soak 50 g of rapeseed seeds.

[0030] Specifically, the rapeseed variety is Qingza No. 4.

[0031] Specifically, the rapeseed seeds are dry rapeseed seeds.

[0032] The present invention has the following beneficial effects:

[0033] The present invention provides a seed soaking agent capable of promoting the early maturity of rapeseed and increasing the yield of rapeseed, which contains kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diphosphate triammonium salt. Before sowing rapeseed seeds, soaking the rapeseed seeds with the seed soaking agent can effectively shorten the whole growth period of rapeseed, achieve the early maturity of rapeseed, and meet the needs of planting rapeseed in temperature and light resource tight balance areas and constraint areas. In addition, the seed soaking agent of the present invention can also effectively improve the seedling rate and effective plant number of rapeseed, and increase the yield of rapeseed. Based on the seed soaking agent of the present invention, the present invention also provides a method for promoting the early maturity of rapeseed and / or increasing the yield of rapeseed. Compared with the method for promoting the early maturity of rapeseed by cultivation means, the method of the present invention is simple to operate, uses less seed soaking agent, and does not need to consume too much labor cost. The present invention is conducive to expanding the planting area of ​​rapeseed and increasing the yield of rapeseed. DETAILED DESCRIPTION

[0034] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0035] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0036] The rapeseed variety Qingza 4 used in the embodiments of the present invention was provided by the College of Agriculture of South China Agricultural University.

[0037] The kaurenoic acid used in the embodiments of the present invention was purchased from Yuanye Company with the product number T98885 and the CAS number 6730-83-2; the zearalenone used was purchased from Aladdin Company with the product number Z100960 and the CAS number 17924-92-4; the phosphatidylethanolamine used was purchased from Maclean Company with the product number P856654 and the CAS number 39382-08-6; the dimethylallyl diphosphate triammonium salt used was purchased from Aladdin Company with the product number D353736 and the CAS number 1186-30-7.

[0038] Example 1 Preparation of Seed Soaking Agent 1 and Its Use Method

[0039] This embodiment provides a seed soaking agent, which is recorded as seed soaking agent 1, and is composed of kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diammonium phosphate. In the seed soaking agent, the weight percentage of kaurenic acid is 27.5%; the weight percentage of zearalenone is 25.1%; the weight percentage of phosphatidylethanolamine is 24.3%; and the weight percentage of dimethylallyl diammonium phosphate is 23.1%.

[0040] The method for using the seed soaking agent is as follows: prepare a solution by mixing the prepared seed soaking agent with water at a weight ratio of 1:75-85, and soak rapeseed seeds in the prepared solution for 8-12 hours at room temperature (25°C±5°C) before germination and sowing; wherein, 45-55g of rapeseed seeds can be soaked in the solution prepared with 1g of the seed soaking agent.

[0041] Example 2 Preparation of Seed Soaking Agent 2 and Its Use Method

[0042] This embodiment provides a seed soaking agent, which is recorded as seed soaking agent 2, and is composed of kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diammonium phosphate. In the seed soaking agent, the weight percentage of kaurenic acid is 29.7%; the weight percentage of zearalenone is 24.4%; the weight percentage of phosphatidylethanolamine is 23.6%; and the weight percentage of dimethylallyl diammonium phosphate is 22.3%.

[0043] The method for using the seed soaking agent is as follows: prepare a solution by mixing the prepared seed soaking agent with water at a weight ratio of 1:75-85, and soak rapeseed seeds in the prepared solution for 8-12 hours at room temperature (25°C±5°C) before germination and sowing; wherein, 45-55g of rapeseed seeds can be soaked in the solution prepared with 1g of the seed soaking agent.

[0044] Example 3 Preparation of Seed Soaking Agent 3 and Its Use Method

[0045] This embodiment provides a seed soaking agent, which is recorded as seed soaking agent 3, and is composed of kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diammonium phosphate. In the seed soaking agent, the weight percentage of kaurenic acid is 28.7%; the weight percentage of zearalenone is 24.7%; the weight percentage of phosphatidylethanolamine is 24.0%; and the weight percentage of dimethylallyl diammonium phosphate is 22.6%.

[0046] The method for using the seed soaking agent is as follows: prepare a solution by mixing the prepared seed soaking agent with water at a weight ratio of 1:75-85, and soak rapeseed seeds in the prepared solution for 8-12 hours at room temperature (25°C±5°C) before germination and sowing; wherein, 45-55g of rapeseed seeds can be soaked in the solution prepared with 1g of the seed soaking agent.

[0047] Example 4 Preparation of Seed Soaking Agent 4 and Its Use Method

[0048] This embodiment provides a seed soaking agent, which is recorded as seed soaking agent 4, and is composed of kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diammonium phosphate. In the seed soaking agent, the weight percentage of kaurenic acid is 28.2%; the weight percentage of zearalenone is 25.1%; the weight percentage of phosphatidylethanolamine is 23.6%; and the weight percentage of dimethylallyl diammonium phosphate is 23.1%.

[0049] The method for using the seed soaking agent is as follows: prepare a solution by mixing the prepared seed soaking agent with water at a weight ratio of 1:75-85, and soak rapeseed seeds in the prepared solution for 8-12 hours at room temperature (25°C±5°C) before germination and sowing; wherein, 45-55g of rapeseed seeds can be soaked in the solution prepared with 1g of the seed soaking agent.

[0050] Example 5 A method for promoting early maturity of rapeseed and increasing rapeseed yield

[0051] Based on the seed soaking agent described in Examples 1 to 4 of the present invention, the present invention also provides a method for promoting early maturity of rapeseed and increasing rapeseed yield. The method is: take 200g of the prepared seed soaking agent, add 16kg of water to prepare a solution, soak 10kg of rapeseed seeds in the prepared solution for 10 hours at room temperature (25℃±5℃), wash with water after soaking, soak the seeds and germinate until they turn white, dry the water on the surface of the seeds, put them in a refrigerator (low-temperature freezer) at -4℃ to -1℃ for 14 days, and then carry out normal rapeseed sowing and cultivation management.

[0052] The present invention uses the seed soaking agent described in Examples 1 to 4 to perform the same soaking treatment on the same batch of rapeseed seeds (recorded as treatments 1 to 4 in sequence), and then performs germination and sowing according to the above method. At the same time, the present invention sets a water group (recorded as CK1) and a gibberellin group (recorded as CK2) as controls, which differ from the treatment group in that the solution used for the seed soaking treatment is a solution prepared by water / gibberellin of equal mass.

[0053] During the cultivation period, the whole growth period (the time from sowing to maturity of rapeseed), seedling rate and yield as well as yield components (number of effective plants, number of siliques per plant, number of siliques per plant and 1000-grain weight) of rapeseed in each treatment group and the control group were measured and recorded. The measurement method was based on Experiments in Crop Cultivation (2014), edited by Chen Xinhong and published by Nanjing University Press.

[0054] The effects of the treatment groups and the control group on the whole growth period, seedling rate and yield of rapeseed and its components are shown in Table 1.

[0055] Table 1 Effects of treatment and control group on the whole growth period, seedling rate and yield of rapeseed and its components

[0056]

[0057] In the table, a and b represent the results of biostatistical analysis; if the letters are the same, such as a, it means there is no difference between the control and treatment groups; if the letters are different, such as b for the control and a for the treatment, it means there is a significant difference between the control and treatment groups (p < 0.05).

[0058] As shown in Table 1, compared with the control, the use of the seed soaking agent of the present invention to soak rapeseed seeds and then germinate and sow can significantly shorten the entire growth period of rapeseed and promote early maturity of rapeseed. The effect of shortening the entire growth period of rapeseed is better than gibberellin. In addition, the seed soaking agent of the present invention can also significantly increase the seedling rate of rapeseed, increase its effective plant number and yield (p < 0.05).

[0059] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A seed soaking agent, characterized in that Contains kaurenic acid, zearalenone, phosphatidylethanolamine and triammonium dimethylallyl diphosphate.

2. The seed soaking agent according to claim 1, characterized in that The seed soaking agent consists of kaurenic acid, zearalenone, phosphatidylethanolamine and dimethylallyl diphosphate triammonium salt.

3. The seed soaking agent according to claim 2, characterized in that The weight percentage of kaurenic acid is 27.5% to 29.7%, the weight percentage of zearalenone is 24.4% to 25.1%, the weight percentage of phosphatidylethanolamine is 23.6% to 24.3%, and the weight percentage of dimethylallyl diphosphate triammonium salt is 22.3% to 23.1%.

4. Use of the seed soaking agent according to any one of claims 1 to 3 in improving the rapeseed seedling rate or in preparing a product for improving the rapeseed seedling rate.

5. Use of the seed soaking agent according to any one of claims 1 to 3 in increasing the number of effective rapeseed plants or in preparing a product for increasing the number of effective rapeseed plants.

6. Use of the seed soaking agent according to any one of claims 1 to 3 in shortening the entire growth period of rapeseed or in preparing a product for shortening the entire growth period of rapeseed.

7. Use of the seed soaking agent according to any one of claims 1 to 3 in increasing rapeseed yield or in preparing a product for increasing rapeseed yield.

8. A method for promoting early maturity of rapeseed and / or increasing rapeseed yield, characterized in that: The seed soaking agent according to any one of claims 1 to 3 and water are prepared into a solution at a weight ratio of 1:75 to 85, and rapeseed seeds are soaked in the prepared solution for 8 to 12 hours before germination and sowing.

9. The method according to claim 8, characterized in that: The solution prepared with 1g of seed soaking agent can soak 45-55g of rapeseed seeds.

10. The method according to claim 8, characterized in that: After germination, dry the surface moisture of the seeds and place them at -4℃~-1℃ for 12~16 days.

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