Pesticide composition for preventing and treating cucumber bacterial angular leaf spot

Abstract

The invention belongs to the technical field of prevention and treatment of cucumber bacterial angular leaf spot, and particularly relates to a pesticide composition for preventing and treating cucumber bacterial angular leaf spot. The invention relates to application of a pesticide composition to prevention and treatment of cucumber bacterial angular leaf spot. The pesticide composition is formed by compounding cytosintepeptidamycin and zinc thiazole or bromothalonil. According to the bactericidal composition disclosed by the invention, cytosintepeptidamycin is compounded with zinc thiazole or bromothalonil, and a compounding test finds that the combined action type of a compounding formula on cucumber bacterial angular leaf spot germs is a synergistic effect, and compared with a single agent, the bactericidal composition can improve the prevention and treatment effect on the cucumber bacterial angular leaf spot germs.

Description

Technical Field

[0001] The invention belongs to the technical field of preventing and treating bacterial angular spot of cucumber, and particularly relates to a pesticide composition for preventing and treating bacterial angular spot of cucumber. Background Art

[0002] Cucumber is one of the main edible vegetables with the largest planting area in my country. Its bacterial angular leaf spot is a bacterial disease caused by Pseudomonas syringae pv. lachrymans. This pathogen can occur almost throughout the entire growth period of cucumber, mainly infecting cucumber leaves, but also stems, petioles and tendrils, and sometimes also harming fruits. The disease occurs at a moderate or above level all year round. In severe cases, the disease rate can reach 100%, seriously affecting the yield and economic benefits of cucumber.

[0003] Although my country has carried out the screening of disease-resistant germplasm resources for cucumber bacterial angular leaf spot, the application of disease-resistant germplasm resources in cucumber breeding is still relatively limited, and highly resistant cucumber varieties are still extremely lacking. At present, chemical control is still the most effective and quickest way to control cucumber bacterial angular leaf spot, but there are few drugs on the market for the prevention and control of bacterial diseases, mainly concentrated in copper preparations and agricultural antibiotics, with a single variety, and with the long-term application of drugs, the prevention effect gradually decreases. Faced with the increasingly severe prevention and control pressure, some producers have adopted inappropriate means such as double the amount of use, which has aggravated the development of pathogen resistance, caused excessive pesticide residues, and also increased environmental pressure. Therefore, conducting pesticide combination experiments is of great significance for preventing and controlling cucumber bacterial angular leaf spot, delaying pathogen resistance, and overcoming the problems of excessive pesticide residues and environmental pressure.

[0004] Cytosinpeptidemycin is a cytosine nucleoside agricultural antibiotic and fungicide, which can inhibit the proliferation of viruses in plants, inhibit the ability of viruses to absorb proteins, and inhibit the synthesis of viral nucleic acids; at the same time, it can induce plants to produce disease resistance, induce plants to produce PR proteins, and induce the activity of defense enzymes in plants to increase. Prior art has reported that cytosinpeptidemycin has a high preventive effect on diseases caused by fungi, bacteria and viruses on a variety of crops.

[0005] So far, there are no reports on the combination of pyrimidine antibiotics with zinc thiazole or bromothionil.

[0006] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0007] The purpose of the present invention is to provide a pesticide composition for preventing and treating bacterial angular leaf spot of cucumber, so as to solve the problem that there are few agents for preventing and treating bacterial diseases on the market, which are mainly copper preparations and agricultural antibiotics, with a single variety, and the prevention effect gradually decreases with the long-term application of the agents.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] The invention discloses an application of a pesticide composition for preventing and treating bacterial angular leaf spot of cucumber. The pesticide composition is prepared by compounding pyrimidine antibiotics with zinc thiazole or bromothionil.

[0010] Preferably, the mass ratio of pyrimidinemycin to zinc thiazole is 1-9:18-1.

[0011] Preferably, the mass ratio of pyrimidinemycin to bromothionil is 1-36:12-1.

[0012] The present invention also provides a pesticide preparation, which includes auxiliary components and the pesticide composition. A person skilled in the art can select different auxiliary components according to the dosage form of the pesticide preparation, and the auxiliary components can be selected from one or more of the dispersants, wetting agents, solvents, cosolvents, emulsifiers, inert fillers, defoamers, antifreeze agents, penetrants, thickeners, synergists and pigments in the prior art.

[0013] Preferably, the content of the pesticide composition is 3-52% by mass.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The present invention compounds pyrimidine antibiotics with zinc thiazole or bromothionil, and through compounding experiments, it is found that the combined action type of the compound formula on cucumber bacterial angular spot pathogen is a synergistic effect, and compared with a single agent, the control effect of cucumber bacterial angular spot disease can be improved. Based on this, a medicament for preventing and treating bacterial diseases can be developed, thereby enriching the types of medicaments; and the medicament contains two active ingredients, which can delay the drug resistance of pathogens and reduce the risk of drug resistance. DETAILED DESCRIPTION

[0016] The following is a clear and complete description of the technical solution of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Pesticide combination combined action test

[0018] 1. Test pathogens

[0019] Pseudomonas syringae pv. lachrymans was isolated from infected cucumber leaf samples in a cucumber planting area.

[0020] Pick a single colony of the test pathogen in 30 mL NB medium and culture it at 20°C and 200 r / min until the logarithmic growth phase. Then adjust the concentration of the bacterial suspension to 1×10 7 cfu / mL, for future use.

[0021] 2. Culture medium

[0022] NA medium: 5 g peptone, 3 g beef extract, 5 g sucrose, 15 g agar powder, distilled water to 1000 mL, heat to melt, adjust to pH 7.0 with 10 mol / L NaOH, and sterilize at 121°C for 20 min.

[0023] NB medium: 5 g peptone, 3 g beef extract, 5 g sucrose, distilled water to 1000 mL, heat to melt, adjust to pH 7.0 with 10 mol / L NaOH, and sterilize at 121°C for 20 min.

[0024] 3. Test reagents

[0025] 30% pyrimidine mycin technical (Dalian Guanfa Pharmaceutical Co., Ltd.), 95% thiazole zinc technical (Zhejiang Xinnong Chemical Co., Ltd.), 95% bromothionil technical (Jiangsu Tuoqiu Agrochemical Co., Ltd.)

[0026] The pyrimidine mycin stock drug is diluted with a 0.1% (v / v) Tween-80 aqueous solution to form a single-dose stock solution; the thiazole zinc stock drug and the bromothionil stock drug are dissolved and diluted with a 0.1% (v / v) Tween-80 aqueous solution to form a single-dose stock solution. Multiple groups of proportions are set, and each single dose and each group of mixed drugs are set with 5 series of mass concentration gradients according to the equal ratio method for standby use.

[0027] 4. Test methods

[0028] The plate count method was used to determine the toxicity of single and mixed agents to the test pathogens. Under sterile conditions, 1 mL of the drug solution and 8 mL of NA medium cooled to 40°C were taken, mixed thoroughly in a 9 cm diameter culture dish, cooled, and made into a drug-containing NA plate. A drug-free NA plate made of 1 mL of 0.1% (v / v) Tween-80 aqueous solution and 8 mL of NA medium was used as a control. Take 50 μL of the prepared bacterial suspension and inoculate it on the drug-containing NA plate and the drug-free NA plate respectively. After smearing evenly, place each plate at 28°C for culture, observe regularly, and mark the single colonies visible to the naked eye with a marker. Each treatment was repeated 4 times; after 48 hours, the number of colonies in each treatment was counted, and the average of the 4 replicates was taken as the measured colony number of the treatment, and the antibacterial rate was calculated.

[0029]

[0030] 4. Data Analysis

[0031] The least square method was used to calculate the toxicity regression equation (y = ax + b) of the probability value of the inhibition rate and the logarithmic value of the test agent concentration, and the inhibition median concentration EC of each treatment agent was obtained. 50 The co-toxicity coefficient (CTC value) of each mixture was calculated using Sun Yunpei co-toxicity coefficient method.

[0032] The synergistic effect of drug mixing was evaluated based on the co-toxicity coefficient (CTC), that is, CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0033] Table 1 The combined effect of pyrimidine antibiotics and zinc thiazole on cucumber bacterial angular leaf spot pathogen

[0034] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Pyrimidine 52.9604 100.000 -- -- Zinc Thiazole 26.5281 199.6389 -- -- Pyrimidine 1: Thiazole zinc 18 18.2631 289.9858 194.3947 149.1737 Pyrimidine 1: Thiazole zinc 12 20.4724 258.6917 191.9743 134.7533 Pyrimidine 1: Thiazole zinc 9 16.0372 330.2347 189.6750 174.1056 Pyrimidine 1: Thiazole zinc 3 24.3959 217.0873 174.7292 124.2422 Pyrimidine 1: Thiazole zinc 1 27.9990 189.1510 149.8194 126.2527 Pyrimidine 3: Thiazole zinc 1 32.5442 162.7338 124.9097 130.2811 Pyrimidine 9: Thiazole zinc 1 35.6113 148.7180 109.9639 135.2425

[0035] As shown in Table 1, the co-toxicity coefficients of pyrimidine antibiotics and zinc thiazole in the mass ratio range of 1-9:18-1 are all greater than 120, that is, the combined effect of pyrimidine antibiotics and zinc thiazole on cucumber bacterial angular leaf spot fungus is a synergistic effect.

[0036] Table 2 The combined effect of pyrimidine antibiotics and bromothionil on cucumber bacterial angular leaf spot pathogen

[0037] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Pyrimidine 52.9604 100.000 -- -- Bromothionil 16.8239 314.7926 -- -- Pyrimidine 1: bromothionil 12 9.6061 551.3205 298.2701 184.8393 Pyrimidine 1: bromothionil 9 5.4120 978.5735 293.3134 333.6273 Pyrimidine 1: bromothionil 3 12.7399 415.7050 261.0945 159.2163 Pyrimidine 1: Bromothionil 1 14.0396 377.2216 207.3963 181.8844 Pyrimidine 3: bromothionil 1 8.1495 649.8607 153.6982 422.8162 Pyrimidine 9: Bromothionil 1 13.5903 389.6926 121.4793 320.7894 Pyrimidine 12: Bromothionil 1 21.6005 245.1814 116.5225 210.4154 Pyrimidine 18: Bromothionil 1 24.1524 219.2759 111.3049 197.0048 Pyrimidine 24: Bromothionil 1 38.4033 137.9059 108.5917 126.9948 Pyrimidine 36: Bromothionil 1 29.3207 180.6246 105.8052 170.7143

[0038] As shown in Table 2, the co-toxicity coefficients of pyrimidine antibiotics and bromothionil in the mass ratio range of 1-36:12-1 are all greater than 120, that is, the combined effect of pyrimidine antibiotics and bromothionil on cucumber bacterial angular leaf spot is a synergistic effect.

[0039] In summary, when the pyrimidine antibiotics of the present invention are compounded with zinc thiazole or bromothionil within a certain mass ratio range, the co-toxicity coefficient is higher than 120, and the combined action type on cucumber bacterial angular leaf spot is a synergistic effect. Compared with a single agent, the control effect on cucumber bacterial angular leaf spot can be improved.

Claims

1. An application of a pesticide composition for preventing and treating bacterial angular spot of cucumber, characterized in that: The pesticide composition is prepared by compounding pyrimidine mycin with zinc thiazole or bromothionil.

2. The use according to claim 1, characterized in that: The mass ratio of pyrimidine mycin to zinc thiazole is 1-9:18-1.

3. The use according to claim 1, characterized in that: The mass ratio of pyrimidine mycin to bromothionil is 1-36:12-1.

4. A pesticide preparation, characterized in that: The pesticide preparation comprises auxiliary components and the pesticide composition according to claim 1.

5. The pesticide formulation according to claim 4, characterized in that: Calculated by mass percentage, the content of the pesticide composition is 3-52%.

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