Burkholderia cepacia and method for leaching rare earth elements in waste by using burkholderia cepacia

Abstract

The invention relates to the technical field of environment-friendly biological rare earth recovery, in particular to burkholderia cepacia and a method for leaching rare earth elements in waste by using the burkholderia cepacia. Specifically, the invention provides a method for leaching rare earth elements in waste by using microorganisms and metabolites thereof, and solves the problems of large dosage of chemical reagents, high production cost, serious environmental pollution and the like in the chemical leaching process of rare earth waste. The strain provided by the invention is low in culture cost, high in growth speed and simple in leaching condition, has the capability of leaching rare earth ions in various wastes, and has a relatively good application prospect in the aspects of rare earth waste microorganism recovery and reutilization and the like.

Description

Technical Field

[0001] The invention relates to the technical field of environmentally friendly biological rare earth recovery, in particular to Burkholderia cepacia and a method for leaching rare earth elements in waste materials. Background Art

[0002] Rare earth elements include fifteen lanthanide elements and two metallic elements, scandium and yttrium. As an important strategic resource, they are widely used in cutting-edge research and high-tech industries and are known as "industrial gold". However, due to their uneven distribution and limited reserves, with the widespread application of rare earth elements in permanent magnetic materials, industrial catalysis and other fields, it has become necessary to extract rare earths from such secondary rare earth resources.

[0003] Traditional methods for recovering REEs from urban minerals mainly use physical and chemical leaching methods using strong acids and organic solvents. However, these methods are complex, energy-intensive, and cause significant environmental pollution. Therefore, there is an urgent need to develop a new clean and efficient leaching technology to ensure the leaching rate of rare earth elements while minimizing environmental pollution.

[0004] Bioleaching using microorganisms is an environmentally friendly alternative. This method mainly dissolves rare earth elements from solid matrices through organic acids (such as citric acid and lactic acid) produced by heterotrophic bacteria or fungi. However, commercial microorganisms such as yeast and Gluconobacter oxydans are inefficient and time-consuming to recover rare earth elements from discarded FCC catalysts and NdFeB magnets. This is mainly because such microorganisms have poor tolerance to high concentrations of rare earth ions. Therefore, screening and cultivating powerful microorganisms from the microbial flora associated with rare earth mines and rare earth wastes is crucial to improving the bioenrichment efficiency of rare earth elements. However, there are few reports on bioleaching based on such microorganisms. Therefore, providing a new Burkholderia cepacia strain that can efficiently leach rare earth ions from waste materials provides new options and approaches for biometallurgy, which is of great practical significance. Summary of the invention

[0005] In view of this, the present invention provides a new Burkholderia cepacia strain that can efficiently leach rare earth ions from waste materials, providing a new option and approach for biometallurgy. The present invention also provides a method for leaching rare earth elements from waste materials using microorganisms and their metabolites, solving the problems of large amounts of chemical reagents, high production costs, and serious environmental pollution in the process of chemical leaching of rare earth waste.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides Burkholderia contaminans AHA-5, whose deposit number is CGMCC No.30396.

[0008] In a second aspect, the present invention also provides a method for culturing the Burkholderia contaminans AHA-5, wherein the Burkholderia contaminans AHA-5 is selected and cultured in a Luria-Bertani liquid culture medium supplemented with 60 g / L glucose or a nutrient-poor Luria-Bertani liquid culture medium supplemented with 60 g / L glucose at 30° C. and 200 rpm for 2 to 4 days;

[0009] In the nutrient-deficient Luria-Bertani liquid culture medium, the content of the original components of the LB culture medium is 0.05-50%.

[0010] In a third aspect, the present invention also provides one or more of the inactivated bacteria, exosomes or metabolites of Burkholderia contaminans AHA-5.

[0011] In a fourth aspect, the present invention also provides a method for preparing the metabolites of Burkholderia contaminans AHA-5, picking Burkholderia contaminans AHA-5, culturing in a Luria-Bertani liquid culture medium supplemented with 60 g / L glucose or a nutrient-poor Luria-Bertani liquid culture medium supplemented with 60 g / L glucose at 30° C. and 200 rpm for 2-4 days, collecting the culture solution and centrifuging it at 8000 rpm for 5 min, and collecting the supernatant;

[0012] In the nutrient-deficient Luria-Bertani liquid culture medium, the content of the original components of the LB culture medium is 0.05-50%.

[0013] In a fifth aspect, the present invention also provides a metabolite obtained by the preparation method.

[0014] In a sixth aspect, the present invention further provides the use of any one of the following in leaching rare earth elements in waste materials;

[0015] (I), the Burkholderia contaminans AHA-5;

[0016] (II), one or more of the inactivated bacteria, exosomes or metabolites;

[0017] (III) the metabolites.

[0018] In some specific embodiments of the present invention, the waste material includes one or more of waste FCC catalyst, waste NdFeB sludge or waste NdFeB powder.

[0019] In some specific embodiments of the present invention, the main framework of the spent FCC catalyst is aluminosilicate, containing rare earth elements and / or transition elements, the content of the rare earth elements and / or transition elements is 0.01% to 10%; the rare earth elements include but are not limited to lanthanum and / or cerium; the transition elements include but are not limited to nickel and / or vanadium;

[0020] The main component of the waste NdFeB sludge is NdFeB (Nd2Fe 14 B) magnet, containing 1% to 30% lubricating oil;

[0021] The main component of the waste NdFeB powder is Nd2Fe 14 B), produced in various links of industrial production of NdFeB magnets, including cutting, compression molding or sintering processes.

[0022] In a seventh aspect, the present invention further provides a product, comprising any of the following:

[0023] (I), the Burkholderia contaminans AHA-5;

[0024] (II), one or more of the inactivated bacteria, exosomes or metabolites;

[0025] (III), the metabolites;

[0026] The products include microecological products, probiotic products, synbiotic products and / or postbiotic products.

[0027] In an eighth aspect, the present invention also provides a method for leaching rare earth elements from waste materials, comprising mixing any one of the following with the waste materials and incubating;

[0028] (I), the Burkholderia contaminans AHA-5;

[0029] (II), one or more of the inactivated bacteria, exosomes or metabolites;

[0030] (III), the metabolites;

[0031] (IV) the product.

[0032] In some specific embodiments of the present invention, the waste material comprises one or more of waste FCC catalyst, waste NdFeB sludge or waste NdFeB powder;

[0033] Preferably, the main framework of the spent FCC catalyst is aluminosilicate, containing rare earth elements and / or transition elements, the content of the rare earth elements and / or transition elements is 0.01% to 10%; the rare earth elements include but are not limited to lanthanum and / or cerium; the transition elements include but are not limited to nickel and / or vanadium;

[0034] The main component of the waste NdFeB sludge is NdFeB (Nd2Fe 14 B) magnet, containing 1% to 30% lubricating oil;

[0035] The main component of the waste NdFeB powder is Nd2Fe 14 B), produced in various links of industrial production of NdFeB magnets, including cutting, compression molding or sintering processes;

[0036] Preferably, the liquid-solid ratio of the Burkholderia contaminans AHA-5 to the waste FCC catalyst is 1:(2-500);

[0037] The liquid-solid ratio of the Burkholderia contaminans AHA-5 to the waste NdFeB oil sludge is 1:(4-500);

[0038] The liquid-to-solid ratio of the Burkholderia contaminans AHA-5 to the waste NdFeB powder is 1:(4-500);

[0039] Preferably, the liquid-to-solid ratio of the metabolites to the spent FCC catalyst is 1:(2-500);

[0040] The liquid-to-solid ratio of the metabolites to the waste NdFeB sludge is 1:50;

[0041] The liquid-to-solid ratio of the metabolite to the waste NdFeB powder is 1:50;

[0042] Preferably, the incubation condition is 30° C. and 200 rpm for 2 to 8 days.

[0043] The present invention provides a method for leaching rare earth elements in waste materials using microorganisms and their metabolites, which solves the problems of large amount of chemical reagents, high production cost, serious environmental pollution, etc. in the process of chemical leaching of rare earth waste. The strain provided by the present invention has low cultivation cost, fast growth rate, simple leaching conditions, and has the ability to leach rare earth ions in a variety of waste materials. It has good application prospects in the recovery and reuse of rare earth waste microorganisms.

[0044] Biological Deposit Description

[0045] Strain: AHA-5; the deposit date is April 22, 2024, the deposit number is CGMCC No.30396, the classification name is Burkholderia contaminans, the name of the depository is: General Microbiology Center of China Microbiological Culture Collection Administration, and the address of the depository is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0047] Figure 1 The figure shows the leaching amount of rare earth elements from spent FCC catalyst by strain AHA-5;

[0048] Figure 2 The bar graph shows the leaching amount of rare earth elements in waste NdFeB sludge by AHA-5 strain;

[0049] Figure 3 A bar graph showing the leaching amount of rare earth elements from waste NdFeB powder by strain AHA-5. DETAILED DESCRIPTION

[0050] The present invention discloses Burkholderia cepacia and a method for leaching rare earth elements from waste materials. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve the desired effect. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in the present invention. The methods and applications of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0051] The present invention adopts the following technical solution:

[0052] (1) Screening of leaching strains: The strains were screened using silica-dissolving medium solid plates. Burkholderia contaminans AHA-5 (CGMCC NO.: 30396), which can produce an obvious silica-dissolving transparent ring on silica-dissolving medium solid plates, was selected for subsequent research.

[0053] (2) Cultivation of microorganisms: Burkholderia cepacia AHA-5 was cultured in a Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose or a nutrient-poor Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original component content of the LB medium was 0.05-50%) at 30° C. and 200 rpm for 2-4 days.

[0054] (3) Co-cultivation to leach rare earth elements from spent FCC catalysts (the main framework is aluminosilicate, containing rare earth elements such as lanthanum and cerium and transition elements such as nickel and vanadium, with a content of 0.01%-10%): Burkholderia cepacia AHA-5 is co-incubated with spent FCC catalysts at a liquid-to-solid ratio of 1:2-1:500 in a Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose or a nutrient-poor Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original component content of the LB medium is 0.05-50%), and cultured at 30°C and 200 rpm for 2-4 days.

[0055] (4) Co-cultivation leaching of waste NdFeB sludge (main component is NdFeB (Nd2Fe 14 B) magnet, containing 1%-30% rare earth elements in lubricating oil): Burkholderia cepacia AHA-5 is co-incubated with waste NdFeB sludge at a liquid-to-solid ratio of 1:4-1:500 in Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose or in nutrient-poor Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original component content of the LB medium is 0.05-50%), and cultured at 30°C, 200 rpm for 2-8 days.

[0056] (5) Co-cultivation leaching of waste NdFeB powder (main component is NdFeB (Nd2Fe 14B), rare earth elements produced in various links of industrial production of NdFeB magnets, such as cutting, pressing, sintering process, etc.): Burkholderia cepacia AHA-5 is co-incubated with waste NdFeB sludge at a liquid-solid ratio of 1:4-1:500 in Luria-Bertani (LB) liquid culture medium supplemented with 60g / L glucose or nutrient-poor Luria-Bertani (LB) liquid culture medium supplemented with 60g / L glucose (the original component content of LB culture medium is 0.05-50%), and cultured at 30°C, 200rpm for 2-8 days.

[0057] (6) Preparation of microbial culture metabolites: The microbial culture solution in step (2) was centrifuged at 8000 rpm for 5 min to collect the supernatant.

[0058] (7) Leaching rare earth elements from waste FCC catalyst (the main framework is aluminosilicate, containing rare earth elements such as lanthanum and cerium and transition elements such as nickel and vanadium, with a content of 0.01%-10%) using microbial culture metabolites: After the supernatant of the leaching solution in step (5) is diluted with ultrapure water (minimum supernatant content is 2%), the original solution or the diluted solution is mixed with the waste FCC catalyst at a liquid-to-solid ratio of 1:2-1:500, and leaching is carried out at 30° C. and 200 rpm to obtain a leaching solution after 2-4 days.

[0059] (8) Leaching of waste NdFeB sludge (main component is NdFeB (Nd2Fe 14 B) magnet, containing 1%-30% rare earth elements in lubricating oil): after the supernatant of the leaching solution in step (5) is diluted with ultrapure water (minimum supernatant content 2%), the original solution or the diluted solution is mixed with the waste NdFeB sludge in a liquid-to-solid ratio of 1:50, and leaching is carried out at 30°C and 200rpm to obtain a leaching solution after 2 days.

[0060] (9) Leaching of waste NdFeB powder (main component is NdFeB (Nd2Fe 14 B), rare earth elements produced in various links of industrial production of NdFeB magnets, such as cutting, compression molding, sintering process, etc.): after the supernatant of the leaching solution in step (5) is diluted with ultrapure water (minimum supernatant content is 2%), the original solution or the diluted solution is mixed with the waste NdFeB sintering or compression molding powder in a liquid-to-solid ratio of 1:50, and leaching is carried out at 30°C and 200rpm to obtain a leachate after 2 days.

[0061] (10) The leaching capacity of AHA-5 was identified by detecting the content and type of rare earth elements in steps (3)(4)(5)(7)(8) through ICP-OES and ICP-MS.

[0062] The strain provided by the present invention has low culture cost, fast growth rate, simple leaching conditions, and the ability to leach rare earth ions in various wastes. It has good application prospects in the recovery and reuse of rare earth waste microorganisms.

[0063] The raw materials and reagents used in the Burkholderia cepacia and the method for leaching rare earth elements from waste materials provided by the invention can all be purchased from the market.

[0064] The present invention will be further described below in conjunction with embodiments:

[0065] Example 1 Screening of rare earth leaching strains and determination of their ability to dissolve insoluble silicates

[0066] A single colony of the purified Burkholderia contaminans AHA-5 was picked from the LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was carried out overnight at 30°C and 200 rpm in a constant temperature shaking incubator. 2 μL of the bacterial liquid was added dropwise to a silica-dissolving medium solid culture plate. After ten days of culture, the results were observed and recorded. The appearance of a transparent ring was recorded as positive, indicating that the strain had the ability to dissolve insoluble silicates. The strain was biologically preserved and the preservation number was CGMCC No. 30396.

[0067] Example 2 Leaching of rare earth elements from spent FCC catalysts by co-cultivation of AHA-5 microorganisms

[0068] A single colony of purified AHA-5 (deposited as CGMCC No. 30396) was picked from a LB solid medium containing 1.5-3% agar and placed in a 4 mL LB liquid medium. The culture was carried out overnight at 30°C and 200 rpm in a constant temperature shaking incubator until the OD value reached 0.8-1.2 as a seed solution. A 1% volume ratio of the seed solution (final OD value 0.008-0.012) was inoculated into a LB liquid medium containing 60 g / L of glucose, which contained a waste FCC catalyst (the main framework was aluminosilicate, containing rare earth elements such as lanthanum and cerium, and transition elements such as nickel and vanadium, with a content of 0.01%-10%) at a solid-liquid ratio of 1:2-1:500. The culture was carried out at 30°C and 200 rpm in a constant temperature shaking incubator for 2-4 days. The supernatant was collected by centrifugation at 8000 rpm for 5 min.

[0069] After the solution becomes clear and transparent, dilute and fix the volume to a target element content of 1ppb-10ppm, and conduct quantitative analysis of the rare earth elements in it by ICP-OES and ICP-MS (refer to the national standard GB / T18115-2021 Chemical analysis method for rare earth impurities in rare earth metals and their oxides for determination).

[0070] Table 1

[0071] Solid-liquid ratio Leaching rate 1:2 21.34％ 1:5 42.84％ 1:10 95.49％ 1:50 97.03％ 1:100 99.78％ 1:500 99.98％

[0072] Example 3 Leaching of rare earth elements from spent FCC catalysts by co-cultivation of AHA-5 microorganisms

[0073] A single colony of purified AHA-5 (deposit number CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and placed in 4 mL LB liquid medium, and cultured overnight at 30°C, 200 rpm constant temperature shaking incubator until the OD value reached 0.8-1.2 as seed liquid. A 1% volume ratio of seed liquid (final OD value 0.008-0.012) was inoculated into a nutrient-poor Luria-Bertani (LB) liquid medium (the original component content of the LB medium was 0.05-50%) with 60 g / L glucose added, and the medium contained a waste FCC catalyst (the main framework was aluminosilicate, containing rare earth elements such as lanthanum and cerium, and transition elements such as nickel and vanadium, with a content of 0.01%-10%) with a liquid-solid ratio of 1:2-1:500. Cultured in a constant temperature shaking incubator at 30°C, 200 rpm for 2-4 days. Centrifuged at 8000 rpm for 5 min to collect the supernatant.

[0074] Example 4 AHA-5 co-cultivation leaching of rare earth elements from waste NdFeB oil sludge

[0075] A single colony of purified AHA-5 (deposit number CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and placed in 4 mL LB liquid medium. The culture was incubated overnight at 30°C and 200 rpm in a constant temperature shaking incubator until the OD value reached 0.8-1.2 as seed liquid. A 1% volume ratio of seed liquid (final OD value 0.008-0.012) was inoculated into LB liquid medium containing 60 g / L of glucose, which contained waste NdFeB oil sludge (the main component of which is NdFeB (Nd2Fe 14 B) magnet, containing 1%-30% lubricating oil). Cultivate in a constant temperature shaking incubator at 30°C and 200 rpm for 2-8 days. Centrifuge at 8000 rpm for 5 min to collect the supernatant.

[0076] Example 5 AHA-5 co-cultivation leaching of rare earth elements from waste NdFeB oil sludge

[0077] A single colony of purified AHA-5 (deposit number CGMCC No. 30396) was picked from the LB solid medium containing 1.5-3% agar and placed in 4 mL LB liquid medium, and cultured overnight at 30°C, 200 rpm in a constant temperature shaking incubator until the OD value reached 0.8-1.2 as a seed solution. A 1% volume ratio of the seed solution (final OD value 0.008-0.012) was inoculated into a nutrient-poor Luria-Bertani (LB) liquid medium (the original component content of the LB medium was 0.05-50%) supplemented with 60 g / L glucose, the medium containing waste NdFeB oil sludge (the main component of which is NdFeB (Nd2Fe 14 B) magnet, containing 1%-30% lubricating oil). Cultivate in a constant temperature shaking incubator at 30°C and 200 rpm for 2-8 days. Centrifuge at 8000 rpm for 5 min to collect the supernatant.

[0078] Example 6 AHA-5 co-cultivation leaching of rare earth elements from waste NdFeB powder

[0079] A single colony of purified AHA-5 (deposit number CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and placed in 4 mL LB liquid medium. The culture was continued overnight at 30°C and 200 rpm in a constant temperature shaking incubator until the OD value reached 0.8-1.2 as seed liquid. A 1% volume ratio of seed liquid (final OD value 0.008-0.012) was inoculated into LB liquid medium containing 60 g / L glucose, which contained waste NdFeB powder (the main component of which is NdFeB (Nd2Fe 14 B), produced in various links of industrial production of NdFeB magnets, such as cutting, pressing, sintering, etc.) Cultivate in a constant temperature shaking shaker at 30°C and 200rpm for 2-8 days. Centrifuge at 8000rpm for 5min to collect the supernatant.

[0080] Example 7 AHA-5 co-cultivation leaching of rare earth elements from waste NdFeB powder

[0081] A single colony of purified AHA-5 (deposited as CGMCC No. 30396) was picked from the LB solid medium containing 1.5-3% agar and placed in 4 mL LB liquid medium, and cultured overnight at 30°C, 200 rpm in a constant temperature shaking incubator until the OD value reached 0.8-1.2 as a seed solution. A 1% volume ratio of the seed solution (final OD value 0.008-0.012) was inoculated into a nutrient-poor Luria-Bertani (LB) liquid medium (the original component content of the LB medium was 0.05-50%) supplemented with 60 g / L glucose, the medium containing waste NdFeB powder (the main component of which is NdFeB (Nd2Fe14 B), produced in various links of industrial production of NdFeB magnets, such as cutting, pressing, sintering, etc.) Cultivate in a constant temperature shaking shaker at 30°C and 200rpm for 2-8 days. Centrifuge at 8000rpm for 5min to collect the supernatant.

[0082] Example 8 Preparation of AHA-5 microbial extract

[0083] From the LB solid medium containing 1.5-3% agar, a single colony of purified AHA-5 (deposited as CGMCC No. 30396) was picked and placed in 4 mL LB liquid medium, and cultured overnight at 30°C, 200 rpm in a constant temperature shaking incubator until the OD value reached 0.8-1.2 as seed liquid. A 1% volume ratio of the seed liquid (final OD value 0.008-0.012) was inoculated into Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose, and cultured at 30°C, 200 rpm in a constant temperature shaking incubator until the logarithmic phase. The supernatant was collected by centrifugation at 8000 rpm for 5 min.

[0084] Example 9 Preparation of AHA-5 microbial extract

[0085] From the LB solid medium containing 1.5-3% agar, a single colony of purified AHA-5 (deposit number CGMCC No. 30396) was picked and placed in 4 mL LB liquid medium, and cultured overnight at 30°C, 200 rpm in a constant temperature shaking incubator until the OD value reached 0.8-1.2 as seed liquid. A 1% volume ratio of seed liquid (final OD value 0.008-0.012) was inoculated into a nutrient-poor Luria-Bertani (LB) liquid medium (the original component content of the LB medium was 0.05-50%), and cultured at 30°C, 200 rpm in a constant temperature shaking incubator until the logarithmic phase. The supernatant was collected by centrifugation at 8000 rpm for 5 minutes.

[0086] Example 10 Leaching of rare earth elements from FCC using supernatant of AHA-5 microbial culture

[0087] The microbial culture supernatant stock solution or diluted solution (diluted with ultrapure water, with a minimum supernatant content of 2%) in Example 8 is added to the spent FCC catalyst (the main framework is aluminosilicate, containing rare earth elements such as lanthanum and cerium and transition elements such as nickel and vanadium, with a content of 0.01%-10%), mixed at a liquid-to-solid ratio of 1:2-1:500, placed in a constant temperature shaking table at 30°C and 200rpm, and a leachate is obtained after 2 days.

[0088] Example 11 Leaching of rare earth elements from waste NdFeB sludge using AHA-5 microbial culture supernatant

[0089] The microbial culture supernatant in Example 8 or the diluted solution (diluted with ultrapure water, the minimum supernatant content is 2%) is added to the waste NdFeB oil sludge (the main component is NdFeB (Nd2Fe 14 B) magnet, containing 1%-30% lubricating oil), mixed according to the liquid-solid ratio of 1:2-1:500, placed in a constant temperature shaking shaker at 30°C and 200rpm, and an leaching solution was obtained after 2 days.

[0090] Example 12 Leaching of rare earth elements from waste NdFeB powder using AHA-5 microbial culture supernatant

[0091] The microbial culture supernatant in Example 8 or the diluted solution (diluted with ultrapure water, the minimum supernatant content is 2%) is added to the waste NdFeB powder (the main component is NdFeB (Nd2Fe 14 B), produced in various links of industrial production of NdFeB magnets, such as cutting, compression molding, sintering process, etc.), mixed according to the liquid-solid ratio of 1:2-1:500, placed in a constant temperature shaking table at 30°C and 200rpm, and the leaching solution was obtained after 2 days.

[0092] Example 13: Leaching of rare earth elements from FCC using AHA-5 microbial culture supernatant

[0093] The microbial culture supernatant stock solution or diluted solution (diluted with ultrapure water, with a minimum supernatant content of 2%) in Example 9 is added to the spent FCC catalyst (the main framework is aluminosilicate, containing rare earth elements such as lanthanum and cerium and transition elements such as nickel and vanadium, with a content of 0.01%-10%), mixed at a liquid-solid ratio of 1:2-1:500, placed in a constant temperature shaking shaker at 30°C and 200rpm, and a leachate is obtained after 2 days.

[0094] Example 14: Leaching of rare earth elements from waste NdFeB oil sludge using AHA-5 microbial culture supernatant

[0095] The microbial culture supernatant in Example 9 or the diluted solution (diluted with ultrapure water, the minimum supernatant content is 2%) is added to the waste NdFeB oil sludge (the main component is NdFeB (Nd2Fe 14 B) magnet, containing 1%-30% lubricating oil), mixed according to the liquid-solid ratio of 1:2-1:500, placed in a constant temperature shaking shaker at 30°C and 200rpm, and an leaching solution was obtained after 2 days.

[0096] Example 15 Leaching of rare earth elements from waste NdFeB powder using AHA-5 microbial culture supernatant

[0097] The microbial culture supernatant in Example 9 or the diluted solution (diluted with ultrapure water, the minimum supernatant content is 2%) is added to the waste NdFeB powder (the main component is NdFeB (Nd2Fe 14B), produced in various links of industrial production of NdFeB magnets, such as cutting, compression molding, sintering process, etc.), mixed according to the liquid-solid ratio of 1:2-1:500, placed in a constant temperature shaking table at 30°C and 200rpm, and the leaching solution was obtained after 2 days.

[0098] Example 16 Detection of the leaching ability of AHA-5 by ICP-MS

[0099] The leachate in Examples 2 to 7, 10 to 15 was filtered with a 0.22 μm needle filter membrane, and the filtrate was collected. A nitric acid-perchloric acid (10:1) mixed acid system was used for digestion on a 210-degree hot plate. After the solution became clear and transparent, it was diluted to a constant volume until the target element content was 1 ppb-10 ppm, and the rare earth elements therein were quantitatively analyzed by ICP-OES and ICP-MS (refer to the national standard GB / T 18115-2021 Chemical analysis method for rare earth impurities in rare earth metals and their oxides for determination). The experimental results are shown in Figures 1 to 3 , Tables 2 to 4.

[0100] Table 2 Leaching amount of rare earth elements from spent FCC catalyst by strain AHA-5

[0101]

[0102] Normal co-culture conditions vs. microbial metabolites (Example 10) p = 0.005;

[0103] Normal co-culture conditions vs. microbial metabolites (Example 13) p = 0.001;

[0104] Normal co-culture conditions vs. nutrient-deficient co-culture conditions p<0.001;

[0105] Microbial metabolites (Example 10) vs. co-culture under poor nutrient conditions p = 0.450;

[0106] Microbial metabolites (Example 10) vs. microbial metabolites (Example 13) p = 0.959;

[0107] Microbial metabolites (Example 13) vs. co-cultivation under poor nutrient conditions p=0.114.

[0108] As shown in Table 2:

[0109] The leaching rate of the co-culture group under normal conditions provided in Example 2 was 59.08%;

[0110] The leaching rate of the microbial metabolite group provided in Example 10 was 91.96%. The microbial metabolite group provided in Example 10 was significantly different from the normal condition co-culture group provided in Example 2 (P<0.01).

[0111] The leaching rate of the microbial metabolite group provided in Example 13 was 93.72%, which was significantly different from the normal condition co-culture group provided in Example 2 (P<0.01).

[0112] The leaching rate of the nutrient-deficient co-culture group provided in Example 3 was 97.03%. The nutrient-deficient co-culture group provided in Example 3 was significantly different from the normal co-culture group provided in Example 2 (P<0.01).

[0113] There was no significant difference between the nutrient-deficient co-culture group provided in Example 3 and the microbial metabolite group provided in Example 10 (P>0.05).

[0114] There was no significant difference between the nutrient-deficient co-culture group provided in Example 3 and the microbial metabolite group provided in Example 13 (P>0.05).

[0115] The leaching rate of the microbial metabolite group provided in Example 10 was 91.96%, and the leaching rate of the microbial metabolite group provided in Example 13 was 93.72%. There was no significant difference between the microbial metabolite group provided in Example 10 and the microbial metabolite group provided in Example 13 (P>0.05).

[0116] Table 3 Bar graph of the leaching amount of rare earth elements from waste NdFeB sludge by AHA-5 strain

[0117]

[0118] Normal co-culture conditions vs. microbial metabolites (Example 11) p = 0.032;

[0119] Normal co-culture conditions vs. microbial metabolites (Example 14) p = 0.569;

[0120] Normal co-culture conditions vs. nutrient-deficient co-culture conditions p = 0.041;

[0121] Microbial metabolites (Example 11) vs. co-culture under nutrient-deficient conditions p<0.001;

[0122] Microbial metabolites (Example 11) vs. microbial metabolites (Example 14) p = 0.015;

[0123] Microbial metabolites (Example 14) vs. co-cultivation under poor nutrient conditions p=0.007.

[0124] As shown in Table 3:

[0125] The leaching rate of the co-culture group under normal conditions provided in Example 4 was 83.14%;

[0126] The leaching rate of the microbial metabolite group provided in Example 11 was 66.33%. The microbial metabolite group provided in Example 11 was significantly different from the normal condition co-culture group provided in Example 4 (P<0.05).

[0127] The leaching rate of the microbial metabolite group provided in Example 14 was 80.35%. There was no significant difference between the microbial metabolite group provided in Example 14 and the normal condition co-culture group provided in Example 4 (P>0.05).

[0128] The leaching rate of the nutrient-deficient co-culture group provided in Example 5 was 96.00%. The nutrient-deficient co-culture group provided in Example 5 was significantly different from the normal co-culture group provided in Example 4 (P<0.05).

[0129] Compared with the microbial metabolite group provided in Example 11, the co-culture group under the nutrient-deficient condition provided in Example 5 had a very significant difference (P<0.01).

[0130] Compared with the microbial metabolite group provided in Example 14, the co-culture group under the nutrient-deficient condition provided in Example 5 had a very significant difference (P<0.01).

[0131] The leaching rate of the microbial metabolite group provided in Example 11 is 66.33%, and the leaching rate of the microbial metabolite group provided in Example 14 is 80.35%. There is a significant difference between the microbial metabolite group provided in Example 11 and the microbial metabolite group provided in Example 14 (P<0.05).

[0132] Table 4 Bar graph of the leaching amount of rare earth elements from waste NdFeB powder by AHA-5 strain

[0133]

[0134] Normal co-culture conditions vs. microbial metabolites (Example 12) p = 0.01;

[0135] Normal co-culture conditions vs. microbial metabolites (Example 15) p<0.001;

[0136] Normal co-culture conditions vs. nutrient-deficient co-culture conditions p<0.001;

[0137] Microbial metabolites (Example 12) vs. co-culture under poor nutrient conditions p = 0.003;

[0138] Microbial metabolites (Example 12) vs. microbial metabolites (Example 15) p<0.001;

[0139] Microbial metabolites (Example 14) vs. co-cultivation under poor nutrient conditions p=0.15.

[0140] As shown in Table 4:

[0141] The leaching rate of the co-culture group under normal conditions provided in Example 6 was 56.80%;

[0142] The leaching rate of the microbial metabolite group provided in Example 12 was 69.44%. The microbial metabolite group provided in Example 12 was significantly different from the normal condition co-culture group provided in Example 6 (P<0.05).

[0143] The leaching rate of the microbial metabolite group provided in Example 15 was 96.76%. The microbial metabolite group provided in Example 15 was significantly different from the normal condition co-culture group provided in Example 6 (P<0.01).

[0144] The leaching rate of the nutrient-deficient co-culture group provided in Example 7 was 93.84%, which was significantly different from the nutrient-deficient co-culture group provided in Example 7 and the normal co-culture group provided in Example 6 (P<0.01).

[0145] Compared with the microbial metabolite group provided in Example 12, the co-culture group under the nutrient-deficient condition provided in Example 7 had a very significant difference (P<0.01).

[0146] Compared with the microbial metabolite group provided in Example 15, the co-culture group under the nutrient-deficient condition provided in Example 7 had a very significant difference (P<0.01).

[0147] The leaching rate of the microbial metabolite group provided in Example 12 is 69.44%, and the leaching rate of the microbial metabolite group provided in Example 15 is 96.76%. The microbial metabolite group provided in Example 12 is significantly different from the microbial metabolite group provided in Example 15 (P < 0.01).

[0148] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Burkholderia contaminans AHA-5, characterized in that Its deposit number is CGMCC No.30396.

2. The method for culturing Burkholderia contaminans AHA-5 according to claim 1, characterized in that: Burkholderia cepacia AHA-5 was selected and cultured in a Luria-Bertani liquid medium supplemented with 60 g / L glucose or a nutrient-poor Luria-Bertani liquid medium supplemented with 60 g / L glucose at 30°C and 200 rpm for 2-4 days; In the nutrient-deficient Luria-Bertani liquid culture medium, the content of the original components of the LB culture medium is 0.05-50%.

3. One or more of the inactivated bacteria, exosomes or metabolites of Burkholderia contaminans AHA-5 as claimed in claim 1.

4. The method for preparing the metabolite of Burkholderia contaminans AHA-5 according to claim 1, characterized in that: Burkholderia cepacia AHA-5 was selected, cultured in a Luria-Bertani liquid medium supplemented with 60 g / L glucose or a nutrient-poor Luria-Bertani liquid medium supplemented with 60 g / L glucose at 30° C. and 200 rpm for 2 to 4 days, the culture solution was collected and centrifuged at 8000 rpm for 5 minutes, and the supernatant was collected; In the nutrient-deficient Luria-Bertani liquid culture medium, the content of the original components of the LB culture medium is 0.05-50%.

5. The metabolite obtained by the preparation method according to claim 4.

6. Use of any of the following in leaching rare earth elements from waste materials; (I) Burkholderia contaminans AHA-5 as described in claim 1; (II) one or more of the inactivated bacteria, exosomes or metabolites according to claim 3; (III) The metabolite according to claim 5.

7. The use according to claim 6, characterized in that The waste material includes one or more of waste FCC catalyst, waste NdFeB sludge or waste NdFeB powder.

8. The use according to claim 7, characterized in that The main framework of the spent FCC catalyst is aluminosilicate, containing rare earth elements and / or transition elements, the content of the rare earth elements and / or transition elements is 0.01% to 10%; the rare earth elements include but are not limited to lanthanum and / or cerium; the transition elements include but are not limited to nickel and / or vanadium; The main component of the waste NdFeB sludge is NdFeB (Nd2Fe 14 B) magnet, containing 1% to 30% lubricating oil; The main component of the waste NdFeB powder is Nd2Fe 14 B), produced in various links of industrial production of NdFeB magnets, including cutting, compression molding or sintering processes.

9. A product, characterized in that Includes any of the following: (I) Burkholderia contaminans AHA-5 as described in claim 1; (II) one or more of the inactivated bacteria, exosomes or metabolites according to claim 3; (III) The metabolite according to claim 5; The products include microecological products, probiotic products, synbiotic products and / or postbiotic products.

10. A method for leaching rare earth elements from waste materials, characterized in that: Take any of the following and mix with waste material and incubate; (I) Burkholderia contaminans AHA-5 as described in claim 1; (II) one or more of the inactivated bacteria, exosomes or metabolites according to claim 3; (III) The metabolite according to claim 5; (IV) The product as claimed in claim 9; Preferably, the waste material comprises one or more of waste FCC catalyst, waste NdFeB sludge or waste NdFeB powder; Preferably, the main framework of the spent FCC catalyst is aluminosilicate, containing rare earth elements and / or transition elements, the content of the rare earth elements and / or transition elements is 0.01% to 10%; the rare earth elements include but are not limited to lanthanum and / or cerium; the transition elements include but are not limited to nickel and / or vanadium; The main component of the waste NdFeB sludge is NdFeB (Nd2Fe 14 B) magnet, containing 1% to 30% lubricating oil; The main component of the waste NdFeB powder is Nd2Fe 14 B), produced in various links of industrial production of NdFeB magnets, including cutting, compression molding or sintering processes; Preferably, the liquid-solid ratio of the Burkholderia contaminans AHA-5 to the waste FCC catalyst is 1:(2-500); The liquid-solid ratio of the Burkholderia contaminans AHA-5 to the waste NdFeB oil sludge is 1:(4-500); The liquid-to-solid ratio of the Burkholderia contaminans AHA-5 to the waste NdFeB powder is 1:(4-500); Preferably, the liquid-to-solid ratio of the metabolites to the spent FCC catalyst is 1:(2-500); The liquid-to-solid ratio of the metabolites to the waste NdFeB sludge is 1:50; The liquid-to-solid ratio of the metabolite to the waste NdFeB powder is 1:50; Preferably, the incubation condition is 30° C. and 200 rpm for 2 to 8 days.

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