Aromatic essential oil composition as well as preparation method and application thereof

Abstract

The invention provides an aromatic essential oil composition as well as a preparation method and application thereof. The aromatic essential oil composition comprises the following raw materials in percentage by weight: 8-12% of wormwood essential oil, 0.5-2% of cedar essential oil, 0.5-2% of tea tree essential oil, 1-2.5% of lavender essential oil, 1-2.5% of sweet orange essential oil, 75-90% of base oil and 1-5% of a modifier which is tea cellulose nanocrystals. The tea cellulose nanocrystal is used as a modifier, so that the stability of the aromatic essential oil is enhanced, the volatilization of the aromatic essential oil is delayed, the utilization efficiency is improved, and the service time is prolonged, thereby widening the application range of the aromatic essential oil composition containing wormwood essential oil in aromatherapy.

Description

Technical Field

[0001] The invention belongs to the field of essential oil production, and particularly relates to a fragrance essential oil composition and a preparation method and application thereof. Background Art

[0002] Aromatherapy is an auxiliary treatment method that uses natural plant fragrances or their extracted essential oils to act on the body through inhalation, massage, bathing, etc. to achieve the effects of mood regulation, stress relief, and sleep improvement. Specifically, aromatherapy using specific plant essential oils can relieve people's depression and anxiety, reduce anxiety and pain in postpartum women, and improve the cognitive ability of Alzheimer's patients. As a key factor in aromatherapy, the research on essential oils is crucial.

[0003] The advantages of aromatic essential oils are mainly good permeability, fast metabolism, no retention, low toxicity, etc., and at the same time, it is easy to pass through the blood-brain barrier, so as to achieve the therapeutic purposes of opening the mind and removing turbidity, promoting blood circulation and removing blood stasis, dispelling wind and cold, relieving muscles and releasing the exterior. At present, the aromatic essential oils commonly used in aromatherapy include lavender essential oil, tea tree essential oil and rose essential oil, etc., while wormwood essential oil is an oily aromatic liquid extracted from the stems and leaves of Artemisia argyi, which has the effects of regulating qi and blood, warming meridians, removing cold and dampness, relieving pain and antibacterial, relieving cough and reducing phlegm, and is also an aromatic essential oil that is easily absorbed by skin penetration. However, it is worth noting that aromatherapy based on wormwood essential oil is not common at present. Because wormwood essential oil is easily oxidized by factors such as air, temperature, and light, and wormwood essential oil is hydrophobic, it has many limitations in its application.

[0004] In order to solve the above problems, people in the prior art often prepare microcapsules with wormwood essential oil as the core material to improve the stability of such essential oils. However, such microcapsule materials containing essential oils have problems such as poor thermal stability, small wall material selectivity, complex preparation process and high cost. Therefore, it is urgent to develop a preparation method for essential oils, which can effectively solve the problems of strong volatility, short use time and poor chemical stability of essential oils, prolong the use effect of essential oils, and broaden their application in aromatherapy. Summary of the invention

[0005] The object of the present invention is to provide a fragrance essential oil composition and a preparation method and application thereof, so as to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: On the one hand, the present invention provides a aromatic essential oil composition, comprising the following raw materials in weight percentage, 8-12% of wormwood essential oil, 0.5-2% of cedar essential oil, 0.5-2% of tea tree essential oil, 1-2.5% of lavender essential oil, 1-2.5% of sweet orange essential oil, 75-90% of base oil and 1-5% of modifier, wherein the modifier is tea cellulose nanocrystals.

[0007] As a further improvement, the raw materials for preparing the tea cellulose nanocrystals include tea leaves, distilled water and sodium chlorite, and the mass ratio of the tea leaves, distilled water and sodium chlorite is 1:(1-10):(0.5-2).

[0008] As a further improvement, the method for preparing the cellulose nanocrystals comprises the following steps:

[0009] S3-1, after the tea leaves are crushed, distilled water is added, and the tea leaves are boiled in a water bath for 10-30 minutes, the tea leaves are fished out, placed in an oven at 50-65°C for 12-48 hours, and then passed through an 80-100 mesh sieve to obtain tea residues, a 2-3 mol / L sodium hydroxide aqueous solution is added to the tea residues, and after the tea residues are bathed in a water bath at 70-80°C for 2-5 hours, a sodium chlorite aqueous solution is added, and the pH value is adjusted to within the range of 3-4. After the tea residues are bathed in a water bath at 70-80°C for 2-5 hours, the supernatant is removed, and the resulting precipitate is post-treated to obtain tea cellulose;

[0010] S3-2, weigh the tea cellulose obtained in step S3-1, add sulfuric acid solution, stir in a water bath, and after the reaction is completed, centrifuge and dialyze until the pH of the external liquid reaches neutral to obtain a suspension intermediate, then ultrasonically disperse the suspension intermediate for 10-30 minutes, and freeze-dry to obtain tea cellulose nanocrystals.

[0011] As a further improvement, the tea leaves in step S3-1 include one or more of jasmine tea, white tea and black tea.

[0012] As a further improvement, the mass concentration of the sulfuric acid solution in step S3-2 is 60-65%, the water bath temperature is 35-65° C., and the water bath reaction time is 20-120 min.

[0013] As a further improvement, the raw materials for preparing the wormwood essential oil include Qichun wormwood and an extractant, and the extractant includes one of propane, butane and dimethyl ether.

[0014] As a further improvement, the preparation step of the wormwood essential oil comprises the following steps:

[0015] S7-1: Take dried Qichun Artemisia argyi leaves and grind them into 20-30 meshes, put them into an extraction tank, add an extractant and evaporate them under reduced pressure for 20-30 minutes to obtain an Artemisia argyi extract;

[0016] S7-2, subjecting the obtained wormwood extract to molecular distillation at a distillation temperature of 50-80° C., allowing the extract to stand for 1-3 hours after the distillation is completed, and enriching the wormwood essential oil.

[0017] As a further improvement, in step S7-1, the extraction temperature is 20-50°C, the extraction pressure is 0.3-0.5 MPa, and the extraction time is 10-30 min.

[0018] On the other hand, the present invention provides a method for preparing a fragrance essential oil composition, comprising the following steps:

[0019] Tea cellulose nanocrystals are dispersed in distilled water, the pH value is adjusted to 6-8, wormwood essential oil, cedar essential oil, tea tree essential oil, lavender essential oil, sweet orange essential oil and base oil are slowly added, a high shear dispersing emulsifier is used to disperse for 40-60 seconds to obtain a crude emulsion, and an ultrasonic cell disruptor is used to ultrasonicate for 15-20 minutes under ice bath conditions to obtain a fragrance essential oil composition.

[0020] The invention provides a fragrance essential oil composition which can be used in aromatherapy.

[0021] Compared with the prior art, the invention has the following beneficial effects: using tea cellulose nanocrystals as a modifier not only improves the stability of the aromatic essential oil composition, delays the volatilization of the aromatic essential oil, improves the utilization efficiency, and prolongs the use time, but also has abundant preparation raw materials, a simple method, and can be adapted to large-scale production. The auxiliary oils and base oils such as wormwood essential oil, cedar essential oil, tea tree essential oil, lavender essential oil, and sweet orange essential oil are used in combination, which broadens the application of the aromatic essential oil composition containing wormwood essential oil in aromatherapy. DETAILED DESCRIPTION

[0022] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following embodiments are examples of the present invention and are only used to illustrate the present invention, but not to limit the present invention. Other combinations and various modifications within the concept of the present invention may be performed without departing from the spirit or scope of the present invention.

[0023] In the following examples, the compound monomers and related reagents used can be purchased from the market, among which commercial cellulose nanocrystals were purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; commercial wormwood essential oil was purchased from Jiangxi Yisenyuan Plant Fragrance Co., Ltd.; white tea was purchased from Fujian Taimei Tea Co., Ltd.; black tea was purchased from Wuyishan Beipao Tea Co., Ltd.; jasmine tea was purchased from Guangxi Hengzhou Century Haosheng Tea Co., Ltd. High shear dispersing emulsifier was purchased from Shanghai Fluke Technology Development Co., Ltd.; ultrasonic cell disruptor was purchased from Ningbo Xinzhi Biotechnology Co., Ltd.

[0024] The following examples and comparative examples all comprise the following steps:

[0025] Preparation of modifier (tea cellulose nanocrystal AC):

[0026] S3-1, add distilled water to the tea leaves, boil the tea leaves in a boiling water bath for 30 minutes, remove the tea leaves, place them in a 65°C oven for 24 hours, pass them through a 90-mesh sieve to obtain tea residues, add 10 mL of a 3 mol / L sodium hydroxide aqueous solution to the tea residues, boil them in a 75°C water bath for 3 hours, add sodium chlorite, and adjust the pH value to 3.8. Continue to boil them in a 75°C water bath for 3 hours, remove the supernatant, and decolorize, wash, and freeze-dry the resulting precipitate to obtain tea cellulose;

[0027] S3-2, add 65% sulfuric acid solution by mass to the tea cellulose obtained in step S3-1, after the reaction is completed by stirring in a water bath at 50°C, centrifuge at 9500r / min for 10min at 25°C, collect the supernatant and put it into a 13kDa dialysis bag, dialyze until the pH of the external liquid reaches neutral to obtain a suspension intermediate, then ultrasonically disperse the suspension intermediate for 20min, and freeze-dry to obtain tea cellulose nanocrystals.

[0028] Table 1

[0029]

[0030]

[0031] Preparation of wormwood essential oil A:

[0032] S7-1: Take 500g of dried Qichun wormwood leaves, grind them into 25 mesh, put them into an extraction tank, add 1L of butane for extraction, the extraction temperature is 40°C, the extraction pressure is 0.45MPa, the extraction time is 30min, and a crude intermediate is obtained. The crude intermediate is then poured into a separation tank for reduced pressure evaporation, the evaporation time is 30min, and the wormwood liquid is separated from the bottom of the separation tank. The obtained liquid is placed at room temperature for 2h to obtain wormwood extract;

[0033] S7-2, subjecting the wormwood extract obtained in step S7-1 to molecular distillation, and after distillation for 1 hour, standing for 2 hours to enrich and obtain a yellow liquid, which is wormwood essential oil A, wherein the conditions for molecular distillation are: evacuating to 100 Pa and setting the distillation temperature to 65°C.

[0034] Preparation of aromatic essential oil compositions

[0035] Tea cellulose nanocrystals are ultrasonically dispersed in 10 ml of distilled water, the pH value is adjusted to 7, wormwood essential oil, cedar essential oil, tea tree essential oil, lavender essential oil, sweet orange essential oil and base oil are slowly added, and a crude emulsion is obtained by dispersing with a FM200A high shear dispersing emulsifier for 60 seconds. The crude emulsion is ultrasonically treated with a JY92-ⅡA ultrasonic cell disruptor in an ice bath for 10 minutes, 5 minutes, and 2 seconds in interval to obtain a fragrance essential oil composition.

[0036] Example 1 provides a fragrance essential oil composition, comprising the following raw materials in weight percentage: 8% of wormwood essential oil A, 1% of cedar essential oil, 1.5% of tea tree essential oil, 1% of lavender essential oil, 2.5% of sweet orange essential oil, 85% of jojoba oil, and 3% of tea cellulose nanocrystals A.

[0037] Example 2 provides a fragrance essential oil composition, comprising 10% wormwood essential oil B, 1% cedar essential oil, 2% tea tree essential oil, 1% lavender essential oil, 1% sweet orange essential oil, 80% jojoba oil, and 5% tea cellulose nanocrystals B.

[0038] Example 3 provides a fragrant essential oil composition, comprising the following raw materials in weight percentage: 11% wormwood essential oil, 1% cedar essential oil, 0.5% tea tree essential oil, 1.5% lavender essential oil, 2% sweet orange essential oil, 83% jojoba oil, and 1% tea cellulose nanocrystals.

[0039] The components and mass of a fragrance essential oil composition provided in Comparative Example 1 are substantially the same as those in Example 1, except that 3% of tea cellulose nanocrystals A are replaced with 3% of commercial cellulose nanocrystals.

[0040] The components and mass of a fragrance essential oil composition provided in Comparative Example 2 are substantially the same as those in Example 1, except that 8% of the wormwood essential oil A is replaced with 8% of commercial wormwood essential oil.

[0041] The test method is as follows:

[0042] Stability test of aromatic essential oils: Accurately weigh 5 g of the aromatic essential oil compositions provided in Examples 1-3 and Comparative Examples 1-2 and 5 g of commercial wormwood essential oil, place them in an oven at 75°C for evaporation for 8 h, 16 h and 24 h, take out and weigh the mass m, calculate the loss rate H of the aromatic essential oil compositions of Examples 1-3 and Comparative Examples 1-2 and the commercial wormwood essential oil,

[0043]

[0044] The test results are shown in Table 2.

[0045] Table 2

[0046]

[0047]

[0048] It can be seen from Examples 1-3 that a fragrance essential oil composition prepared by the raw materials, components and methods provided by the present invention uses wormwood essential oil, cedar essential oil, tea tree essential oil, lavender essential oil and sweet orange essential oil as auxiliary oils, and uses tea cellulose nanocrystals as a modifier, which improves the miscibility between the components and enhances the stability of the fragrance essential oil composition.

[0049] By comparing Comparative Examples 1-2 with Example 1, it can be seen that when the tea cellulose nanocrystals provided by the present invention are used as a modifier and the wormwood essential oil provided by the present invention is used as an auxiliary oil, the prepared aromatic essential oil composition has a 24-hour loss rate of 3%, which is 72% lower than the 24-hour loss rate of 11% of the commercial wormwood essential oil under the same conditions. This shows that this type of aromatic essential oil composition has a certain stability, can prolong the volatilization time of the essential oil, and increase the contact opportunity between the aromatic essential oil composition and the human body, which is more conducive to the application of this type of essential oil in aromatherapy.

[0050] Application test of the aromatic essential oil composition of Examples 1-3 and Comparative Examples 1-2 for aromatherapy: The depression self-rating scale is a simple tool for measuring depression, which usually includes 20 declarative questions. Individuals need to choose an appropriate answer according to the statement of each question. The scale adds the scores of each question to obtain a rough score, multiplies the rough score by 1.25, and rounds off to an integer to obtain a standard score. The higher the score, the more obvious the depression tendency. A score of ≥53 points indicates depression, 53-62 points indicates mild depression, 63-72 points indicates moderate depression, and 72 points or more indicates severe depression. The depression self-rating scale was distributed on the university campus, and 60 college students with depression tendencies were selected from the college students who voluntarily filled in the scale for aromatherapy. The aromatherapy experiment cycle is six consecutive weeks, once a week, each time at the same time (18:30-21:30) and the same place (classroom, room temperature controlled at about 24°C, relative air humidity of 45%), basic data collection and grouping in the first week, statistics of the subjects' gender, age, initial depression self-rating scale, and random division of the subjects into six groups; from the second to the fifth week, the six groups smelled the aromatic essential oil composition and distilled water prepared by Examples 1-3 and Comparative Examples 1-2, respectively, and distilled water was used as the control group. The sniffing time was 3 hours; in the last week, no sniffing experiment was performed, and only the subjects were asked to fill in the final depression self-rating scale. The scores of each subject's initial depression self-rating scale and the final depression self-rating scale were collected, and the average score of each group of subjects was calculated. The specific results are shown in Table 3.

[0051] Table 3

[0052]

[0053] It can be seen from Examples 1-3 that a kind of aromatic essential oil composition provided by the present invention can be used for aromatherapy to relieve the symptoms of anxiety and depression in college students. Among them, when wormwood essential oil, cedar essential oil, tea tree essential oil, lavender essential oil and sweet orange essential oil are used as auxiliary oils, jojoba oil is used as base oil, and tea cellulose nanocrystals are used as modifiers, by smelling this kind of aromatic essential oil composition, the symptoms of depression and anxiety in college students are greatly relieved. It can be seen from the comparison of Example 1 and Comparative Examples 1-2 that when wormwood essential oil and tea cellulose nanocrystals prepared by the method provided by the present invention are used, this kind of aromatic essential oil composition has the greatest degree of relief for the symptoms of depression and anxiety in college students through aromatherapy, and the difference in the score of the depression self-rating scale before and after aromatherapy can reach up to 8.1.

[0054] In summary, the aromatic essential oil composition provided by the present invention adopts tea cellulose nanocrystals as a modifier, thereby improving the stability of the aromatic essential oil combination and increasing the use time; the wormwood essential oil provided by the present invention is used in combination with cedar essential oil, tea tree essential oil, lavender essential oil and sweet orange essential oil, and through aromatherapy, the therapeutic effects of various essential oils are fully exerted, the symptoms of anxiety and depression in college students are effectively alleviated, and the application of the aromatic essential oil composition is further broadened.

[0055] The above implementation modes are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with this technology to understand the content of the present invention and implement it, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A fragrance essential oil composition, characterized in that The aromatic essential oil composition comprises the following raw materials in weight percentage: 8-12% of wormwood essential oil, 0.5-2% of cedar essential oil, 0.5-2% of tea tree essential oil, 1-2.5% of lavender essential oil, 1-2.5% of sweet orange essential oil, 75-90% of base oil and 1-5% of modifier, wherein the modifier is tea cellulose nanocrystal.

2. A fragrance essential oil composition according to claim 1, characterized in that: The raw materials for preparing the tea cellulose nanocrystals include tea leaves, distilled water and sodium chlorite, and the mass ratio of the tea leaves, distilled water and sodium chlorite is 1:(1-10):(0.5-2).

3. A fragrance essential oil composition according to claim 2, characterized in that, The preparation method of tea cellulose nanocrystals comprises the following steps: S3-1, after the tea leaves are crushed, distilled water is added, and the tea leaves are boiled in a water bath for 10-30 minutes, the tea leaves are fished out, placed in an oven at 50-65°C for 12-48 hours, and then passed through an 80-100 mesh sieve to obtain tea residues, a 2-3 mol / L sodium hydroxide aqueous solution is added to the tea residues, and after the tea residues are bathed in a water bath at 70-80°C for 2-5 hours, a sodium chlorite aqueous solution is added, and the pH value is adjusted to within the range of 3-4. After the tea residues are bathed in a water bath at 70-80°C for 2-5 hours, the supernatant is removed, and the resulting precipitate is post-treated to obtain tea cellulose; S3-2, weigh the tea cellulose obtained in step S3-1, add sulfuric acid solution, stir in a water bath, and after the reaction is completed, centrifuge and dialyze until the pH of the external liquid reaches neutral to obtain a suspension intermediate, then ultrasonically disperse the suspension intermediate for 10-30 minutes, and freeze-dry to obtain tea cellulose nanocrystals.

4. A fragrance essential oil composition according to claim 3, characterized in that: The tea leaves in step S3-1 include one or more of jasmine tea, white tea and black tea.

5. A fragrance essential oil composition according to claim 3, characterized in that: The mass concentration of the sulfuric acid solution in step S3-2 is 60-65%, the water bath temperature is 35-65° C., and the water bath reaction time is 20-120 min.

6. A fragrance essential oil composition according to claim 1, characterized in that: The raw materials for preparing the wormwood essential oil include Qichun wormwood leaf and an extractant, and the extractant includes one of propane, butane and dimethyl ether.

7. A fragrance essential oil composition according to claim 6, characterized in that: The preparation steps of the wormwood essential oil comprise the following steps: S7-1: Take dried Qichun Artemisia argyi leaves and grind them into 20-30 meshes, put them into an extraction tank, add an extractant to extract and evaporate under reduced pressure, the reduced pressure evaporation time is 20-30 minutes, and obtain wormwood extract; S7-2, subjecting the prepared wormwood extract to molecular distillation at a distillation temperature of 50-80° C., allowing the extract to stand for 1-3 hours after the distillation is completed, and enriching the wormwood essential oil.

8. A fragrance essential oil composition according to claim 7, characterized in that: In step S7-1, the extraction temperature is 20-50°C, the extraction pressure is 0.3-0.5 MPa, and the extraction time is 10-30 min.

9. A method for preparing a fragrance essential oil composition according to claims 1-8, characterized in that: The following steps are involved: Tea cellulose nanocrystals are dispersed in distilled water, the pH value is adjusted to 6-8, wormwood essential oil, cedar essential oil, tea tree essential oil, lavender essential oil, sweet orange essential oil and base oil are slowly added, a high shear dispersing emulsifier is used to disperse for 40-60 seconds to obtain a crude emulsion, and an ultrasonic cell disruptor is used to ultrasonicate for 15-20 minutes under ice bath conditions to obtain a fragrance essential oil composition.

10. Use of a fragrance essential oil composition according to any one of claims 1 to 8 in aromatherapy.

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