Application of water-soluble tomato concentrate in preparation of composition for promoting wound healing and tissue regeneration

Abstract

The invention belongs to the technical field of biological medicine and food, and particularly relates to application of a water-soluble tomato concentrate in preparation of a composition for promoting wound healing and tissue regeneration. The invention finds that the water-soluble tomato concentrate can promote aggregation of macrophages and neutrophils at a wound part, increase the occurrence rate of neovascularization at the wound part, promote cell proliferation and promote tissue regeneration. The water-soluble tomato concentrate can serve as a functional component to be applied to products for promoting wound healing and tissue regeneration, the effect of the water-soluble tomato concentrate is close to or equivalent to that of human recombinant epidermal growth factors and adenosine, and the water-soluble tomato concentrate is mild in stress reaction, high in safety and wide in application prospect.

Description

Technical Field

[0001] The invention belongs to the field of biomedicine and food technology, and particularly relates to the application of a water-soluble tomato concentrate in preparing a composition for promoting wound healing and tissue regeneration. Background Art

[0002] Wound healing consists of four phases: hemostasis, inflammation, proliferation, and remodeling. These four phases interact with each other and occur sequentially and overlappingly. The hemostasis phase is the first stage of wound healing, during which platelets are activated and aggregate to the wound surface to form a blood clot, rapidly stopping bleeding. Simultaneously, platelets release bioactive factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and transforming growth factor-β (TGF-β), further promoting wound healing.

[0003] After wound hemostasis, the inflammatory phase begins, the body's initial response to injury. Inflammatory cells such as neutrophils and macrophages are recruited to the wound site to clear dead tissue and potential pathogens. The release of inflammatory mediators such as cytokines and chemokines promotes wound cleansing and prepares for the subsequent proliferation phase.

[0004] Once the inflammatory response stabilizes, wound healing enters the proliferation phase. During this phase, fibroblasts begin to proliferate and migrate, producing collagen and extracellular matrix to form granulation tissue. Simultaneously, angiogenesis occurs, with new capillaries growing from existing vessels to supply nutrients and oxygen to the wound. Epithelial cells also begin to migrate, covering the wound surface and achieving re-epithelialization.

[0005] The final phase of wound healing, the tissue remodeling phase, primarily involves remodeling and restoring skin integrity. During this phase, collagen and other extracellular matrix components are further modified and reorganized, with changes in the alignment and crosslinking of collagen fibers, leading to the gradual maturation and softening of scar tissue. This process can continue for months or even years, ultimately resulting in a more stable scar.

[0006] Human recombinant epidermal growth factor (rEGF) is a multifunctional growth factor that can promote the division and proliferation of various tissue cells, thereby accelerating wound healing. rEGF is mainly used to treat skin damage caused by burns, trauma and chronic ulcers, but rEGF is mainly used to repair the epidermis. For deeper tissue damage, it usually needs to be used in combination with other treatments. Although rEGF has shown positive effects in promoting wound healing, its production cost is relatively high and it needs to be stored at low temperatures. At the same time, in some cases, rEGF may cause allergic reactions or other side effects.

[0007] Adenosine is an endogenous nucleoside with multiple biological effects including vasodilation and anti-inflammation. 2A Activation of the receptor can promote fibroblasts to produce collagen, which helps reduce inflammation and accelerate wound healing, but if the activation is excessive, it may cause skin fibrosis.

[0008] The water-soluble tomato concentrate (WSTC) developed by Provis Natural Products Co., Ltd. is known as the "natural aspirin". It has a very strong function of inhibiting platelet aggregation and has no adverse reactions such as gastrointestinal bleeding. It is expected to have very good application prospects. In 2009, EFSA (European Food Safety Authority) first approved the research on the health claim of "water-soluble tomato concentrate helps maintain normal platelet aggregation and contributes to healthy blood flow", and this health claim has been approved. The results of multiple tests have confirmed that water-soluble tomato concentrate contains naturally occurring antiplatelet compounds. After ingestion, it can inhibit the activity of healthy people's platelets and reduce platelet aggregation, thereby keeping the blood in a fluid and low-coagulation state, preventing the formation of microaggregates in the circulatory system and preventing platelets from adhering to blood vessel walls or fatty plaques, thereby maintaining healthy blood circulation. At the same time, platelet function is not completely inhibited and is maintained at an appropriate level, allowing platelets to aggregate when blood vessels are damaged, but will not disrupt the coagulation process necessary after injury. Water-soluble tomato concentrate has been used as a nutritional supplement in Europe. Sold under the brand name.

[0009] However, there are no reports on the use of water-soluble tomato concentrate in preparing compositions for promoting wound healing and tissue regeneration. Summary of the Invention

[0010] Adenosine and recombinant human epidermal growth factor (rheGF) have been shown to promote wound healing. However, during further research, the inventors unexpectedly discovered that the effects of adenosine and rheGF alone on wound healing and tissue regeneration were less pronounced than those achieved with water-soluble tomato concentrate. Consequently, the present invention proposes a method for promoting wound healing and tissue regeneration, namely, using a water-soluble tomato concentrate as an active ingredient to prepare a composition to promote wound healing and tissue regeneration.

[0011] The specific technical solutions are as follows:

[0012] In a first aspect, the present invention provides use of a water-soluble tomato concentrate in preparing a composition for promoting wound healing.

[0013] In a first aspect, the present invention provides use of a water-soluble tomato concentrate in preparing a composition for promoting tissue regeneration.

[0014] In the present invention, the promotion of wound healing is that the water-soluble tomato concentrate of the present invention can promote angiogenesis and cell proliferation at the wound site;

[0015] In the present invention, the water-soluble tomato concentrate promotes wound healing, and is particularly capable of promoting skin damage repair, promoting capillary regeneration on the skin wound surface, and stimulating skin cell proliferation.

[0016] In the present invention, the water-soluble tomato concentrate can promote tissue regeneration and improve tissue condition or function through regeneration, for example, in the case of skin wounds, the elasticity and water retention of the skin can be restored through repair and regeneration.

[0017] Generally speaking, those skilled in the art will understand that wound healing and tissue regeneration are two distinct physiological processes. Wound healing restores function by repairing the structure of damaged tissue, often accompanied by scar formation. Tissue regeneration, on the other hand, refers to the body's ability to completely restore the damaged area by producing new cells and tissues, leaving no visible scar. Tissue regeneration typically occurs in certain organs and tissues, such as the liver and skin. The difference between wound healing and tissue regeneration lies in their biological processes and end results.

[0018] Those skilled in the art will appreciate that the water-soluble tomato concentrate in the composition of the present invention is the main active ingredient. In certain embodiments, the water-soluble tomato concentrate of the present invention is the only active ingredient.

[0019] In some embodiments, a composition containing water-soluble tomato concentrate as the main or only active ingredient is used to prepare food, medicine, health food, or cosmetics. Those skilled in the art can prepare the composition into a specific product form according to actual needs.

[0020] In some embodiments, the dosage forms of the present invention include tablets, pills, granules, powders, soft capsules, hard capsules, gels, sustained-release preparations, pastes, or liquid preparations.

[0021] In some embodiments, the present invention further comprises additives and / or excipients acceptable for use in foods, pharmaceuticals, health foods, and cosmetics. Adding additives and / or excipients can improve the commercial properties of the composition product, including improving ease of delivery and ease of use, improving product stability and extending shelf life, and improving product taste and acceptance.

[0022] In some embodiments, the product of the present invention further comprises other ingredients that promote wound healing and / or tissue regeneration, including one or more of cell growth factors, plant and animal extracts, stem cells, vitamins, and minerals.

[0023] In some embodiments, the preparation method of the water-soluble tomato concentrate comprises: separating heat-stable water-soluble components from tomatoes using a physical method, and then concentrating and desugaring the components to obtain the water-soluble tomato concentrate.

[0024] In the present invention, the characteristic components of the water-soluble tomato concentrate include nucleoside compounds, flavonoid compounds and phenolic acid compounds.

[0025] Beneficial effects

[0026] (1) The water-soluble tomato concentrate provided by the present invention can effectively promote angiogenesis and cell proliferation at the site of injury; it can promote tissue regeneration and can be widely used to repair injuries, especially skin damage, and maintain the health of body tissues and organs.

[0027] (2) The water-soluble tomato concentrate provided by the present invention is derived from edible plant materials and is safe to use without any health risks. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a typical image of new blood vessels at the wound site of zebrafish in the embodiment;

[0029] Figure 2 The incidence of neovascularization at the wound site of zebrafish in the examples (mean ± SE), where * indicates P < 0.05, ** indicates P < 0.01, and *** indicates P < 0.001 compared with the model group;

[0030] Figure 3 This is a typical graph of the fluorescence intensity of proliferating cells in the wound site of zebrafish in the embodiment;

[0031] Figure 4 The fluorescence intensity of proliferating cells in the wound of zebrafish in the examples, where * indicates P < 0.05, ** indicates P < 0.01, and *** indicates P < 0.001 compared with the model group;

[0032] Figure 5 This is a typical diagram of the regenerated area of ​​the zebrafish tail fin in the embodiment;

[0033] Figure 6 The mean ± SE of the regenerated area of ​​the zebrafish caudal fin in the examples is shown. Compared with the model group, * indicates P < 0.05, ** indicates P < 0.01, and *** indicates P < 0.001. DETAILED DESCRIPTION

[0034] The embodiments of the present invention will be described in detail below with reference to the examples, but it will be understood by those skilled in the art that the following examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention. Where specific conditions are not specified in the examples, the methods were performed according to conventional conditions or the conditions recommended by the manufacturer. Where the manufacturers of the reagents or instruments are not specified, they are all conventional products that can be obtained commercially.

[0035] The optional scope of the terms "and / or", "or / and", and "and / or" used in this document includes any one of two or more related listed items, and also includes any and all combinations of the related listed items, and the said any and all combinations include any two related listed items, any more related listed items, or a combination of all related listed items.

[0036] As used herein, "one or more" refers to any one, any two, or any two or more of the listed items.

[0037] In this disclosure, terms such as "first aspect" and "second aspect" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or quantity, nor as implicitly indicating the importance or quantity of the technical features indicated. Furthermore, terms such as "first" and "second" serve only as non-exhaustive enumeration and description and should not constitute a closed-ended limitation on quantity.

[0038] In the present invention, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0039] The percentage concentrations mentioned in the present invention, unless otherwise specified, refer to the final concentration, which refers to the percentage of the added component in the system after the addition of the component.

[0040] The temperature parameters in the present invention, unless otherwise specified, allow for either constant temperature treatment or treatment within a certain temperature range. The constant temperature treatment allows for temperature fluctuations within the precision range controlled by the instrument.

[0041] The room temperature in the present invention generally refers to 4°C to 30°C, preferably 20±5°C.

[0042] At present, water-soluble tomato concentrate is managed as ordinary food in Europe, the United States, and my country.

[0043] Water-soluble tomato concentrate (WSTC) is an extract derived from tomatoes. It is primarily obtained by isolating heat-stable, water-soluble components from tomatoes through physical methods (such as centrifugation and filtration), followed by concentration and desugaring. These heat-stable compounds are its main biologically active ingredients. The water-soluble compounds in WSTC include nucleosides, flavonoids, and phenolic acids. Nucleosides can be nucleosides themselves or nucleotides formed by phosphorylation. Nucleosides may be selected from at least one of uridine, cytidine, adenosine, and guanosine. Nucleosides are more preferably adenosine, adenosine-3'-monophosphate, and / or adenosine-5'-monophosphate. Flavonoids can be flavonoids themselves or their glycosylation products. Flavonoids may be selected from at least one of myricetin, luteolin, kaempferol, and naringenin. Flavonoids are more preferably quercetin-3-O-glucoside, naringin, and / or rutin. The phenolic acid compound can be a phenolic acid compound itself or a glycosylated product thereof. The phenolic acid compound can be cinnamic acid or a derivative thereof, such as p-coumaric acid, caffeic acid, coumaric acid, ferulic acid, or sinapic acid. The phenolic acid compound can be benzoic acid or a derivative thereof, such as p-hydroxybenzoic acid, chlorogenic acid, gallic acid, vanillic acid, or syringic acid. It is understood that the bioactive components in WSTC are not limited to one or more of the aforementioned substances and may also contain other substances.

[0044] Additionally, the water-soluble tomato concentrate of the present invention is lycopene-free or substantially lycopene-free.The water-soluble tomato concentrate is substantially free of water-insoluble particulate matter.

[0045] The water-soluble tomato concentrate of the present invention can be purchased from Provis Natural Products Co., Ltd. or its authorized sales agency. WO 99 / 55350, WO 2010 / 049707, and CN102271697 also disclose methods for producing the water-soluble tomato concentrate that can be used in the present invention. Therefore, the water-soluble tomato concentrate used in the present invention can also be prepared according to WO 99 / 55350, WO 2010 / 049707, or CN102271697. If prepared according to the methods described in WO 99 / 55350, WO 2010 / 049707, or CN102271697, it should be ensured that the ingredients of the prepared water-soluble tomato concentrate meet the above requirements. For example, the water-soluble tomato concentrate can be prepared according to the methods of CN102271697. Figure 2 and Figure 4 The process described is a preferred method for producing a water-soluble tomato concentrate. The prepared water-soluble tomato concentrate may be added with one or more auxiliary materials to improve the stability of the product or the convenience of use.

[0046] Without limitation, the water-soluble tomato concentrate of the present invention can be prepared by the following method, which comprises the following steps:

[0047] (a) preparing an initial mixture of tomato homogenate;

[0048] (b) separating the water-soluble component from the initial mixture, wherein the separation temperature is not higher than 60°C;

[0049] (c) filtering and concentrating the water-soluble component, wherein the concentration temperature is not higher than 60°C.

[0050] Preferably, the temperature of the initial mixture in step (a) is not higher than 35°C.

[0051] Preferably, the pH of the initial mixture in step (a) does not exceed 5.5.

[0052] Preferably, the browning index of the initial mixture does not exceed 0.4 AU at 4% solids, wherein the browning index is defined as the absorbance of the soluble fraction at 420 nm.

[0053] Preferably, in step (b), water-insoluble solids with a particle size greater than 500 μm are separated and removed from mixture A to obtain a water-soluble component.

[0054] Preferably, the separation in step (b) comprises at least one of centrifugal separation and natural sedimentation separation, and the water-soluble component is the supernatant obtained after separation.

[0055] Preferably, in step (c), the filtration is performed using a filter membrane. Without limitation, the pore size of the filter membrane used for filtration is no greater than 1 μm, specifically 0.2 μm.

[0056] Preferably, the concentration in step (c) can be carried out by membrane filtration concentration, low-temperature evaporation concentration, freeze-drying concentration or spray drying concentration.

[0057] Preferably, in the step (c), the step of removing free sugars from the water-soluble components before filtering is further included.

[0058] The water-soluble tomato concentrate used in the embodiments of the present invention was purchased from Provis Natural Products Co., Ltd. Sold under the brand name.

[0059] Example 1:

[0060] This example provides research on the efficacy of water-soluble tomato concentrate in promoting wound healing.

[0061] 1. Experimental cell lines and experimental animals

[0062] Zebrafish were reared in fish farming water at 28°C (water quality: 200 mg of instant sea salt was added to 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO3). They were bred and provided by the Huante Biological Fish Farming Center. The experimental animal use license number is: SYXK (Zhejiang) 2022-0004. The breeding and management of zebrafish complies with the requirements of the International AAALAC accreditation (certification number: 001458) and the IACUC ethics review number is: IACUC-2024-8434-01.

[0063] 2. Reagents

[0064] Sample ①: Water-soluble tomato concentrate The powder is diluted with purified water.

[0065] Human recombinant epidermal growth factor: human recombinant epidermal growth factor, white powder, batch number 20231176-1, Shanghai Haohai Biotechnology Co., Ltd., stored at 4°C, solvent is standard dilution water.

[0066] 3. Detection method

[0067] 3.1 Zebrafish model to evaluate the promoting effect of the test substance on wound healing

[0068] 1) 2-day-old transgenic zebrafish expressing green fluorescent neutrophils and macrophages were randomly selected and plated in 6-well plates, with 30 zebrafish treated in each well (experimental group). Samples were administered in water, along with positive controls of 7.00 μg / mL human recombinant epidermal growth factor and 20.0 μg / mL adenosine. A normal control group and a model control group were also established, with the volume per well being 3 mL. All experimental groups, except the normal control group, received glacial acetic acid injected into the trunk to establish a zebrafish wound healing model. After one day of treatment at 28°C, 10 zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. Data were acquired using NIS-Elements D3.20 advanced image processing software, and macrophage and neutrophil fluorescence intensity at the zebrafish wound sites was analyzed. The efficacy of the samples in promoting wound healing was evaluated using statistical analysis of this indicator. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS 26.0 software. P < 0.05 indicated statistical significance.

[0069] 2) 2-day-old transgenic green fluorescent zebrafish (Fli-1) expressing angiogenesis were randomly selected and plated in 6-well plates, with 30 zebrafish treated in each well (experimental group). The samples were administered in water, along with positive controls of 7.00 μg / mL human recombinant epidermal growth factor and 20.0 μg / mL adenosine. A normal control group and a model control group were also established, with the volume per well being 3 mL. All experimental groups, except the normal control group, received glacial acetic acid via trunk injection to establish a zebrafish wound healing model. After 2 days of treatment at 28°C, 10 zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. The images were saved and the angiogenesis observed in each experimental group was analyzed. The incidence rate (%) was calculated and used to evaluate the wound healing efficacy of the samples. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS 26.0 software. P < 0.05 indicated statistical significance.

[0070] 3) Wild-type AB strain zebrafish (3 dpf) were randomly selected and plated in 6-well plates, with 30 zebrafish treated in each well (experimental group). The samples were administered with water-soluble solutions. Positive controls included 7.00 μg / mL human epidermal growth factor and 20.0 μg / mL adenosine. A normal control group and a model control group were also established, with the volume per well being 3 mL. All experimental groups, except the normal control group, received glacial acetic acid injected into the trunk to establish a zebrafish wound healing model. After treatment at 28°C for 5 hours, the zebrafish were stained using the BRDU kit. Ten zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. Data were acquired using NIS-Elements D 3.20 advanced image processing software. The staining intensity (S) of proliferating cells at the wound site was analyzed, and the wound healing efficacy of the samples was evaluated using statistical analysis of this indicator. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS 26.0 software. P < 0.05 indicated statistical significance.

[0071] Test results:

[0072] 1) Referring to Table 1, Sample ① showed no significant difference in improving the fluorescence intensity of macrophages and neutrophils at the wound site compared to the model group, but a trend toward improvement was observed; this improvement was even more pronounced at the lower concentration (1000 μg / mL). Adenosine, human recombinant epidermal growth factor, and adenosine groups showed significant differences relative to the model control group, suggesting that both groups exert their effects by affecting macrophages and neutrophils. Those skilled in the art will appreciate that more macrophages and neutrophils are not necessarily better; the stimulation of fewer immune cells suggests that the water-soluble tomato concentrate of the present invention is relatively mild in its stress response.

[0073] Table 1: Results of the experimental evaluation of the efficacy of samples in promoting wound healing (macrophage & neutrophil fluorescence intensity) (n=10)

[0074]

[0075] Compared with the model group, * indicates P < 0.05, ** indicates P < 0.01, and *** indicates P < 0.001 in sample group ①.

[0076] 2) See Table 2 and Figure 2 In the angiogenesis test, the water-soluble tomato concentrate significantly increased the number (rate) of blood vessels compared to the model control group, indicating that the water-soluble tomato concentrate can promote wound healing by promoting the increase of new blood vessels in the wound site, increasing blood flow and nutrient supply.

[0077] Table 2 Number (rate) of neovascularization in wounds of different groups (n=30)

[0078]

[0079] * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001

[0080] 3) See Table 3 and Figure 4 In the cell proliferation promotion experiment, the increased fluorescence intensity in the model group relative to the normal group is due to the presence of wounds in the model group, which results in the appearance of proliferating cells at the wound site. The increased fluorescence intensity in the sample and positive groups relative to the model group is due to their stronger wound healing properties, resulting in a significant increase in proliferating cells at the wound site, promoting faster wound healing. The water-soluble tomato concentrate significantly increased the fluorescence intensity of proliferating cells in the wound relative to the model control group. This indicates that the water-soluble tomato concentrate exhibited higher cell proliferation intensity than the model control group, suggesting that the water-soluble tomato concentrate promotes wound healing by enhancing cell proliferation. The significantly increased fluorescence intensity of proliferating cells in sample group ① compared to the recombinant human epidermal growth factor and adenosine groups indicates that the water-soluble tomato concentrate exhibits a stronger cell proliferation-promoting ability than recombinant human epidermal growth factor and adenosine, primarily through promoting cell proliferation.

[0081] Table 3 Effects of different groups on cell proliferation in wound area

[0082]

[0083] Compared with the model group, * indicates P < 0.05, ** indicates P < 0.01, and *** indicates P < 0.001 in sample group ①; compared with human recombinant epidermal growth factor, ## Indicates P < 0.01, ### Indicates P < 0.001; compared with adenosine, △△ Indicates P < 0.01, △△△ indicates P < 0.001;

[0084] 3.2 Zebrafish model to evaluate the promoting effect of test substances on tissue regeneration

[0085] Wild-type AB strain zebrafish (3 days post-fertilization) were randomly selected and plated in 6-well plates. Thirty zebrafish were treated in each well (experimental group). Water-soluble samples were administered, along with a positive control of human recombinant epidermal growth factor (rhREGF) at a concentration of 7.00 μg / mL. A normal control group and a model control group were also established. The volume per well was 3 mL. Except for the normal control group, all other experimental groups used a scalpel to cut the caudal fin to establish a zebrafish caudal fin injury model. The treatment was continued at 28°C for 4 hours. Ten zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Data were analyzed and collected using NIS-Elements D3.20 advanced image processing software. The caudal fin regeneration area was analyzed and statistically analyzed to assess the efficacy of the samples in promoting tissue regeneration. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS 26.0 software. P < 0.05 indicated statistical significance.

[0086] See Table 4, Figure 6 Compared with the model control group, water-soluble tomato concentrate can significantly increase the regeneration area of ​​the zebrafish tail fin, and its efficacy is basically no different from that of adenosine and epidermal growth factor, indicating that water-soluble tomato concentrate has a strong ability to promote tissue regeneration, especially at lower concentrations.

[0087] Table 4 Effects of different groups on the regeneration area of ​​zebrafish tail fin

[0088]

[0089] In summary, the water-soluble tomato concentrate of the present invention has a less potent effect on wound healing than recombinant human epidermal growth factor and adenosine in stimulating immune cells and promoting blood vessel proliferation. However, significant differences were observed compared to the model group, indicating a milder effect, avoiding problems such as excessive immune stimulation and fever. Furthermore, it significantly promotes cell proliferation at the wound site, accelerating wound healing, even exceeding the effects of recombinant human epidermal growth factor and adenosine. This also suggests that the water-soluble tomato concentrate of the present invention primarily exerts its effects by promoting cell proliferation, which in turn enhances metabolic recovery at the wound site. The water-soluble tomato concentrate of the present invention has a slightly less potent effect on tissue regeneration than recombinant human epidermal growth factor, but is comparable to adenosine at lower concentrations. Considering the potential side effects of recombinant human epidermal growth factor, the effectiveness of the water-soluble tomato concentrate as a food is both striking and reassuring.

[0090] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. Application of water-soluble tomato concentrate in preparing a composition for promoting wound healing.

2. Application of water-soluble tomato concentrate in preparing a composition for promoting tissue regeneration.

3. The use according to any one of claims 1 or 2, characterized in that: The composition is used for preparing food, medicine, health food or cosmetics.

4. The use according to claim 3, characterized in that: The food, medicine, health food and cosmetics also include acceptable additives and / or auxiliary materials.

5. The use according to claim 4, characterized in that: The dosage forms of the food, medicine, health food or cosmetics include tablets, pills, granules, powders, soft capsules, hard capsules, gels, sustained-release preparations, pastes, or liquid preparations.

6. The use according to claim 3, characterized in that: The composition also includes other effective ingredients, which include one or more of cell growth factors, animal and plant extracts, stem cells, vitamins and minerals.

7. The use according to claim 1 or 2, characterized in that: The characteristic components of the water-soluble tomato extract are nucleoside compounds, flavonoid compounds and phenolic acid compounds.

8. The use according to claim 7, characterized in that: The water-soluble tomato extract is prepared by separating heat-stable water-soluble components from tomatoes by a physical method, and then concentrating and removing sugar.

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