Storage method and application of cymbidium seeds

Abstract

The invention discloses a storage method and application of cymbidium seeds, and belongs to the technical field of agricultural product storage. According to the method, mixed and sterilized sandy soil and cymbidium orchid seeds are alternately treated at two temperature intervals of 2-6 DEG C and 20-30 DEG C, natural environment temperature change is simulated, seed aging is delayed, and seed vitality, storage stability and germination rate are improved. Meanwhile, mature cymbidium capsules are collected and dried in the shade till the water content of the seeds is smaller than 10%, river sand and matrix soil are mixed and sterilized to serve as a storage medium, the air permeability and the water-retaining property are guaranteed, and the infection risk is reduced; a proper humidity environment is kept by spraying a mixed sterilizing solution, so that the storage effect is further improved; and the effectiveness and the controllability of the storage process are ensured by monitoring the temperature and the humidity in real time. The method is applied to artificial breeding of cymbidium orchid and storage of orchid seeds, the germination rate and the seedling success rate are increased, the genetic stability is kept, and powerful support is provided for production, research and application of cymbidium orchid and orchid.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural product storage, and in particular relates to a storage method and application of cymbidium orchid seeds. Background Art

[0002] Cymbidium faberi, also known as Chinese orchid, Jiuzi orchid, Xialan, Jiuhua orchid, Jiujie orchid, etc., was often called "Hui" in ancient times. It is a terrestrial herbaceous plant belonging to the genus Cymbidium of the Orchidaceae family. It is mainly distributed in Shaanxi, East China, Central South, and Southwest China. As an orchid plant with profound cultural heritage and ornamental value, Cymbidium faberi enjoys a high reputation in China and even in the world. However, the natural reproduction ability of Cymbidium faberi is relatively weak, especially the germination problem of its seeds, which has always been a key factor limiting its artificial reproduction efficiency.

[0003] The seeds of Cymbidium orchid grow in the form of capsules, each of which contains tens of thousands of seeds. These seeds are extremely small, without endosperm, and are not fully developed, with each seed only about 200 microns long. Under natural conditions, Cymbidium orchid seeds are extremely difficult to germinate, and special techniques such as aseptic germination or symbiotic germination are required to achieve this. However, in actual production, Cymbidium orchid seeds need to be stored for 4 to 5 months from ripening and picking in autumn to sowing in the following spring. During this period, the vitality of the seeds is easily affected by storage conditions, resulting in a significant reduction in germination rate and survival rate. At present, conventional storage methods are difficult to effectively maintain the vitality of Cymbidium orchid seeds. For example, simple dry or wet storage methods often cannot accurately control the humidity, temperature and oxygen content of the storage environment, which are crucial for the long-term storage of Cymbidium orchid seeds. In addition, some storage methods may also introduce bacteria or pests, further damaging the quality of the seeds. Given the importance and complexity of Cymbidium orchid seed storage, an effective storage method is urgently needed to ensure the vitality of Cymbidium orchid seeds during storage and improve their germination rate and survival rate during sowing. This storage method should be able to accurately control various parameters of the storage environment, prevent the invasion of bacteria and pests, and maintain the physiological stability of the seeds. Summary of the invention

[0004] In view of the technical problem that traditional storage methods are easily affected by environmental factors during storage, resulting in low seed germination rate and survival rate and short storage period during storage, the present invention aims to provide a storage method and application of Cymbidium orchid seeds.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is: The present invention provides a storage method for cymbidium orchid seeds, comprising the following steps: Step 1: Mix the sterilized sandy soil with the Cymbidium orchid seeds to be stored to obtain a mixture of sandy soil and Cymbidium orchid seeds; Step 2: Place the mixture of sand and Cymbidium orchid seeds in a storage box, sterilize and moisturize, adjust the storage temperature, and store it until before sowing to obtain the stored Cymbidium orchid seeds; The storage temperature is regulated as follows: treating alternately in two temperature ranges of 2°C-6°C and 20-30°C, changing the temperature range every 5-9 days, treating for 60 days in total, and then storing at 2°C-6°C until before sowing.

[0006] In step 1, the specific processing process of the Cymbidium orchid seeds to be stored is: collecting mature Cymbidium orchid pods, drying them in the shade until the moisture content of the seeds is less than 10%, and sieving them.

[0007] The time for collecting mature Cymbidium orchid pods is any period among the Autumnal Equinox period (September 20-September 25), the Cold Dew period (October 5-October 10), the Frost Descent period (October 20-October 25) and the Light Snow period (November 20-November 25).

[0008] Furthermore, the time to collect mature Cymbidium orchid pods is during the autumnal equinox.

[0009] Furthermore, the sieving is through a 40-60 mesh sieve.

[0010] In step 1, the sterilized sandy soil is obtained by mixing river sand and matrix soil and then sterilizing them; the volume ratio of the river sand and matrix soil is 1:1-3, the river sand is river sand that has passed through a 6-10 mesh fine sieve, and the matrix soil is matrix soil that has passed through a 6-10 mesh fine sieve.

[0011] Furthermore, the volume ratio of the river sand to the matrix soil is 1:2.

[0012] The sterilization is specifically carried out by spraying a 1500-fold diluted solution of carbendazim with a mass concentration of 30%.

[0013] In step 1, the volume ratio of the sterilized sand to the Cymbidium seeds to be stored is 3 to 5:1.

[0014] Furthermore, in step 1, the volume ratio of the sterilized sand to the Cymbidium seeds to be stored is 4:1.

[0015] In step 2, the sterilization and humidity maintenance are specifically as follows: spraying a mixed sterilization liquid to make the humidity of the mixture of sand and Cymbidium seeds 70% to 75%, wherein the mixed sterilization liquid is a 1500-fold diluted solution of carbendazim and an 800-fold diluted solution of chloramphenicol in a volume ratio of 1:1.

[0016] Furthermore, the mass concentration of the carbendazim is 30%, and the mass concentration of the chlorpyrifos is 2.5%.

[0017] The storage method further includes monitoring the temperature and humidity of the storage environment, specifically: inserting a thermometer and a hygrometer to a position 3 cm above the upper surface of the bottom layer of sand for monitoring.

[0018] Furthermore, the storage temperature is specifically regulated as follows: 4°C and 25°C are treated alternately, the temperature is exchanged every 7 days, and after a total of 60 days of treatment, the crops are stored at 4°C until before sowing.

[0019] The above-mentioned storage method of Cymbidium orchid seeds is applied in the artificial cultivation of Cymbidium orchid seedlings.

[0020] The above-mentioned storage method of cymbidium orchid seeds is used in the storage of orchid seeds.

[0021] Compared with the prior art, the present invention achieves the following technical effects: The present invention provides a storage method for cymbidium orchid seeds. The storage method comprises mixing sterilized sand and cymbidium orchid seeds and treating them alternately in a specific temperature range, thereby helping to ensure seed vitality, breaking the dormancy of the seeds, promoting the physiological activity of the seeds, and helping to improve the germination rate of the seeds. The strategy of alternating treatment in two temperature ranges of 2℃~6℃ and 20~30℃ simulates the temperature changes in the natural environment, so that the physiological activities inside the seeds maintain a certain fluctuation and balance, thereby delaying the aging process of the seeds and improving the vitality, storage stability and germination rate of the seeds; temperature alternation treatment helps to inhibit the breeding and reproduction of pests and diseases, maintain the moisture balance inside the seeds, maintain the genetic stability of the seeds, and improve the adaptability of the seeds to the environment, thereby avoiding the adverse effects of being in a single temperature range for a long time; after 60 days of temperature alternation treatment, the seeds are stored at 2℃~6℃ until before sowing. The low-temperature storage stage effectively slows down the metabolic rate of the seeds, prolongs the storage life of the seeds, and maintains the vitality of the seeds before sowing at more than 50%. It can also restore the seeds whose vitality has been reduced to less than 10% under traditional storage methods to more than 50%.

[0022] Furthermore, by collecting mature Cymbidium orchid pods and drying them in the shade until the moisture content of the seeds is less than 10%, the seeds are obtained by sieving, thereby ensuring the maturity and dryness of the seeds, reducing the risk of rot due to excessive moisture during storage, and improving the storage stability of the seeds; the best time to collect Cymbidium orchid pods is clarified, and the seeds collected during these periods have higher maturity and quality, thereby improving the vitality of the seeds after storage.

[0023] Furthermore, the seed vigor of Cymbidium orchid pods collected during the autumnal equinox (September 20-September 25) was the highest, reaching 97.8±0.9%, providing a scientific basis for the efficient collection and storage of Cymbidium orchid seeds.

[0024] Furthermore, a suitable storage medium was obtained by mixing river sand with matrix soil and sterilizing it. The mixing ratio of river sand and matrix soil and the screening of matrix soil ensured the air permeability and water retention of the storage medium, which was beneficial to the breathing and moisture retention of seeds; the use of a 1500-fold dilution of 30% metalaxyl for sterilization effectively killed harmful microorganisms in the sand and reduced the risk of infection of seeds during storage.

[0025] Furthermore, by spraying a mixed sterilization liquid, the humidity of the sand and seed mixture is maintained at 70% to 75%, which not only provides the appropriate humidity environment required by the seeds, but also further kills possible microorganisms and improves the storage effect.

[0026] Furthermore, monitoring of temperature and humidity during the storage process is the key to storage; real-time monitoring of the temperature and humidity of the bottom layer of sand and the middle layer of sand can not only effectively reflect the overall temperature and humidity conditions, but also ensure the effectiveness and controllability of the entire storage process.

[0027] The application of the storage method of cymbidium seeds provided by the present invention in the artificial cultivation of cymbidium seedlings provides strong support for the production, research and application of cymbidium and orchid plants by improving the seed germination rate, shortening the seedling cultivation period, improving the success rate of seedling cultivation, ensuring the quality of seedlings, expanding the storage range, improving the storage efficiency, maintaining genetic diversity and other advantages. The cymbidium seed storage method helps to improve the germination rate of cymbidium seeds in artificial cultivation of seedlings and provide a more reliable seed source for seedling cultivation; shortens the period of artificial cultivation of cymbidium seedlings and improves the efficiency of seedling cultivation; optimizes the storage conditions and reduces the loss of seeds during storage and germination, thereby improving the success rate of artificial cultivation of cymbidium seedlings, reducing the cost of seedling cultivation and improving the economic benefits; and maintains the genetic stability of seeds during storage, which helps to ensure the quality of seedlings.

[0028] The storage method of cymbidium seeds provided by the present invention is applied in the storage of seeds of orchid plants. The method of the present invention is not only suitable for the storage of cymbidium seeds, but can also be extended to the storage of seeds of other orchid plants. It not only improves the storage quality and life of seeds, but also improves the germination rate and survival rate of seeds after sowing, provides strong support for the protection and research of orchid plants, and generates significant economic and social benefits. It is of great significance for the long-term storage and genetic improvement of orchid plant germplasm resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a comparison of seed activity at different sampling periods of the present invention, wherein a is seeds sampled during the Autumnal Equinox; b is seeds sampled during the Cold Dew period; c is seeds sampled during the Frost Descent period; d is seeds sampled during the Beginning of Winter; e is seeds sampled during the Light Snow period; f is statistics of seed activity at different periods.

[0030] Figure 2 The results of comparing the vitality of Cymbidium orchid seeds stored by the method of the present invention and the traditional storage method without any treatment are shown in Figure 1. a shows the vitality change of Cymbidium orchid seeds stored by the method of the present invention, and b shows the vitality change of seeds stored by the traditional method without any treatment at room temperature.

[0031] Figure 3 The figures are the results of restoring the vitality of Cymbidium seeds stored according to the method of the present invention; a is the TTC staining result of fresh seeds collected during the autumnal equinox; b is the TTC staining result of seeds stored for 120 days using the traditional method at room temperature without any treatment; c is the TTC staining result of seeds stored for 56 days using the method provided in Example 1; d is the statistical result of seed vitality. DETAILED DESCRIPTION

[0032] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0033] The present invention is described in further detail below: 1. Specific embodiments Example 1 This embodiment provides a storage method for Cymbidium orchid seeds, and the specific preparation steps are as follows: Picking ripe Cymbidium pods: According to local climatic conditions, during the autumnal equinox period (September 20th to September 25th) every year, choose healthy Cymbidium pods and pick them as a whole from the base of the Cymbidium pods. Be careful to avoid damaging the pods during the picking process, and remove shriveled, blackened, and worm-eyed Cymbidium pods.

[0034] Shade drying of Cymbidium orchid pods: Place the picked Cymbidium orchid pods on a drying tray in a dry and ventilated area, and dry them in the shade at room temperature until the moisture content of the seeds is less than 10%. Avoid direct sunlight during the drying process.

[0035] Removing the cymbidium fruit pods to obtain the cymbidium seeds to be stored: After the cymbidium fruit pods are dried, the skin is mechanically broken, the dried fruit pods are peeled open, the seeds are taken out, and the seeds are sieved out with a 40-mesh sieve to obtain the cymbidium seeds to be stored.

[0036] Sand and sterilization treatment: Take river sand that has passed through a 10-mesh fine sieve and mix it with substrate soil in a volume ratio of 2:1. During the mixing process, spray a 1500-fold diluted solution of 30% carbendazim with a mass concentration for sterilization and insecticide treatment to obtain sterilized sand.

[0037] Mixing of sterilized sand and Cymbidium seeds to be stored: Take the Cymbidium seeds to be stored, mix them evenly with the sterilized sand in a volume ratio of 1:4, and place them in a storage box. At the same time, use a 1:1 volume ratio of 30% carbendazim 1500 times diluted solution and a 2.5% mass concentration of chlorpyrifos 800 times diluted solution to mix the sterilization liquid spray, and keep the humidity of the mixture of sand and Cymbidium seeds at 70% to 75%. Regulate the storage temperature: Alternate between 4℃ and 25℃, exchange every 7 days, and after a total of 60 days of treatment, store at 4℃ until sowing. Management of the storage process: Insert the thermometer and hygrometer 3cm above the upper surface of the bottom layer of sand to monitor the temperature and humidity.

[0038] The cymbidium pods were picked during the autumnal equinox, and the vitality of the cymbidium seeds was tested to be 96.28±1.80%. The vitality of the cymbidium seeds to be stored after drying and sieving was 85.44±2.81%. The storage method provided in Example 1 was used to store the cymbidium seeds for 120 days, and the vitality of the cymbidium seeds after storage was tested to be 70.71±1.90%.

[0039] Example 2 This embodiment provides a storage method for Cymbidium orchid seeds, and the specific preparation steps are as follows: Picking ripe Cymbidium pods: According to local climatic conditions, during the autumnal equinox period (September 20th to September 25th) every year, choose healthy Cymbidium pods and pick them as a whole from the base of the pods. Be careful to avoid damaging the pods during the picking process, and remove shriveled, blackened, and worm-eyed Cymbidium pods.

[0040] Shade drying of Cymbidium orchid pods: Place the picked Cymbidium orchid pods on a drying tray in a dry and ventilated area, and dry them in the shade at room temperature until the moisture content of the seeds is less than 10%. Avoid direct sunlight during the drying process.

[0041] Removing the cymbidium fruit pods to obtain the cymbidium seeds to be stored: After the cymbidium fruit pods are dried, the skin is mechanically broken, the dried fruit pods are peeled open, the seeds are taken out, and the seeds are sieved out with a 40-mesh sieve to obtain the cymbidium seeds to be stored.

[0042] Sand and sterilization treatment: Take river sand that has passed through a 10-mesh fine sieve and mix it with substrate soil in a volume ratio of 2:1. During the mixing process, spray a 1500-fold diluted solution of 30% carbendazim with a mass concentration for sterilization and insecticide treatment to obtain sterilized sand.

[0043] Mix the sterilized sand and the Cymbidium seeds to be stored: Take the Cymbidium seeds to be stored, mix them evenly with the sterilized sand in a volume ratio of 1:4, and then place the mixture of sand and Cymbidium seeds in a storage box. At the same time, use a 1:1 volume ratio of 30% carbendazim 1500 times diluted solution and a 2.5% mass concentration of chlorpyrifos 800 times diluted solution to mix the sterilization liquid spray, and keep the humidity of the mixture of sand and Cymbidium seeds at 70% to 75%. Regulate the storage temperature: Alternate between 2℃ and 20℃, exchange every 7 days, and store at 2℃ until sowing after a total of 60 days of treatment. Management of the storage process: Insert the thermometer and hygrometer 3cm above the upper surface of the bottom layer of sand to monitor the temperature and humidity.

[0044] The cymbidium pods were picked during the autumnal equinox, and the vitality of the cymbidium seeds was tested to be 96.28±1.80%. The vitality of the cymbidium seeds to be stored after drying and sieving was 85.44±2.81%. The storage method provided in Example 2 was used to store the cymbidium seeds for 120 days, and the vitality of the cymbidium seeds after storage was tested to be 58.65±1.86%.

[0045] Example 3 This embodiment provides a storage method for Cymbidium orchid seeds, and the specific preparation steps are as follows: Picking ripe Cymbidium pods: According to local climatic conditions, during the autumnal equinox period (September 20th to September 25th) every year, choose healthy Cymbidium pods and pick them as a whole from the base of the pods. Be careful to avoid damaging the pods during the picking process, and remove shriveled, blackened, and worm-eyed Cymbidium pods.

[0046] Shade drying of Cymbidium orchid pods: Place the picked Cymbidium orchid pods on a drying tray in a dry and ventilated area, and dry them in the shade at room temperature until the moisture content of the seeds is less than 10%. Avoid direct sunlight during the drying process.

[0047] Removing the cymbidium fruit pods to obtain the cymbidium seeds to be stored: After the cymbidium fruit pods are dried, the skin is mechanically broken, the dried fruit pods are peeled open, the seeds are taken out, and the seeds are sieved out with a 40-mesh sieve to obtain the cymbidium seeds to be stored.

[0048] Sand and sterilization treatment: Take river sand that has passed through a 10-mesh fine sieve and mix it with substrate soil in a volume ratio of 2:1. During the mixing process, spray a 1500-fold diluted solution of 30% carbendazim with a mass concentration for sterilization and insecticide treatment to obtain sterilized sand.

[0049] Mix sterilized sand and Cymbidium seeds to be stored: Take the Cymbidium seeds to be stored, mix them evenly with the sterilized sand in a volume ratio of 1:4, place the mixture of sand and Cymbidium seeds in a storage box, and spray with a sterilization solution mixed with a 1500-fold dilution of 30% carbendazim and an 800-fold dilution of 2.5% chlorpyrifos in a volume ratio of 1:1, and keep the humidity of the mixture of sand and Cymbidium seeds at 70% to 75%. Regulate the storage temperature: Alternate between 6°C and 30°C, exchange every 9 days, and store at 6°C until sowing after a total of 60 days of treatment. Management of the storage process: Insert the thermometer and hygrometer 3 cm above the upper surface of the bottom layer of sand to monitor the temperature and humidity.

[0050] The cymbidium pods were picked during the autumnal equinox, and the vitality of the cymbidium seeds was tested to be 96.28±1.80%. The vitality of the cymbidium seeds to be stored after drying and sieving was 85.44±2.81%. The storage method provided in Example 3 was used to store the cymbidium seeds for 120 days, and the vitality of the cymbidium seeds after storage was tested to be 50.81±2.73%.

[0051] Example 4 This embodiment provides a storage method for Cymbidium orchid seeds, and the specific preparation steps are as follows: Picking ripe Cymbidium pods: According to local climatic conditions, during the autumnal equinox period (September 20th to September 25th) every year, choose healthy Cymbidium pods and pick them as a whole from the base of the pods. Be careful to avoid damaging the pods during the picking process, and remove shriveled, blackened, and worm-eyed pods.

[0052] Shade drying of Cymbidium orchid pods: Place the picked Cymbidium orchid pods on a drying tray in a dry and ventilated area, and dry them in the shade at room temperature until the moisture content of the seeds is less than 10%. Avoid direct sunlight during the drying process.

[0053] Removing the cymbidium fruit pods to obtain the cymbidium seeds to be stored: After the cymbidium fruit pods are dried, the skin is mechanically broken, the dried fruit pods are peeled open, the seeds are taken out, and the seeds are sieved out with a 40-mesh sieve to obtain the cymbidium seeds to be stored.

[0054] Sand and sterilization treatment: Take river sand that has passed through a 10-mesh fine sieve and mix it with substrate soil in a volume ratio of 2:1. During the mixing process, spray a 1500-fold diluted solution of 30% carbendazim with a mass concentration for sterilization and insecticide treatment to obtain sterilized sand.

[0055] Mix the sterilized sand and the Cymbidium seeds to be stored: Take the Cymbidium seeds to be stored, mix them evenly with the sterilized sand in a volume ratio of 1:4, and then place the mixture of sand and Cymbidium seeds in a storage box. At the same time, use a 1:1 volume ratio of 30% carbendazim 1500 times diluted solution and a 2.5% mass concentration of chlorpyrifos 800 times diluted solution to mix the sterilization liquid spray, and keep the humidity of the mixture of sand and Cymbidium seeds at 70% to 75%. Regulate the storage temperature: Alternate between 5℃ and 26℃, exchange every 5 days, and store at 4℃ until sowing after a total of 60 days of treatment. Management of the storage process: Insert the thermometer and hygrometer 3cm above the upper surface of the bottom layer of sand to monitor the temperature and humidity.

[0056] The cymbidium pods were picked during the autumnal equinox, and the vitality of the cymbidium seeds was tested to be 96.28±1.80%. The vitality of the cymbidium seeds to be stored after drying and sieving was 85.44±2.81%. The storage method provided in Example 4 was used to store the cymbidium seeds for 120 days, and the vitality of the cymbidium seeds after storage was tested to be 66.47±2.99%.

[0057] Example 5 Picking ripe Cymbidium pods: According to local climatic conditions, during the Cold Dew period (October 5th to October 10th) every year, choose healthy Cymbidium pods and pick them as a whole from the base of the pods. Be careful to avoid damaging the pods during the picking process, and remove shriveled, blackened, and worm-eyed Cymbidium pods.

[0058] Shade drying of Cymbidium orchid pods: Place the picked Cymbidium orchid pods on a drying tray in a dry and ventilated area, and dry them in the shade at room temperature until the moisture content of the seeds is less than 10%. Avoid direct sunlight during the drying process.

[0059] Removing the cymbidium fruit pods to obtain the cymbidium seeds to be stored: After the cymbidium fruit pods are dried, the skin is mechanically broken, the dried fruit pods are peeled open, the seeds are taken out, and the seeds are sieved out with a 40-mesh sieve to obtain the cymbidium seeds to be stored.

[0060] Sterilization and insecticide treatment of sandy soil: Take river sand that has passed through a 10-mesh fine sieve and mix it with substrate soil in a volume ratio of 2:1. During the mixing process, spray a 1500-fold diluted solution of 30% carbendazim with a mass concentration to sterilize and insecticide the sandy soil to obtain sterilized sandy soil.

[0061] Mixing of sand and seeds: Take the stored Cymbidium orchid seeds and mix them evenly with the sterilized sand in a volume ratio of 1:3, then place the mixture of sand and Cymbidium orchid seeds in a storage box, and spray with a sterilization solution mixed with a 1500-fold dilution of 30% carbendazim and an 800-fold dilution of 2.5% chlorpyrifos in a volume ratio of 1:1, and keep the humidity of the mixture of sand and Cymbidium orchid seeds at 70% to 75%. Regulate the storage temperature: Alternate between 4°C and 25°C, exchange every 7 days, and store at 4°C until sowing after a total of 60 days of treatment. Management of the storage process: Insert the thermometer and hygrometer 3 cm above the upper surface of the bottom layer of sand to monitor the temperature and humidity.

[0062] The cymbidium fruit pods were picked during the Cold Dew period, and the vitality of the cymbidium seeds was tested to be 84.3±1.5%. The vitality of the cymbidium seeds to be stored after drying and sieving was 69.07±3.31%. The storage method provided in Example 5 was used to store the cymbidium seeds for 120 days, and the vitality of the cymbidium seeds after storage was tested to be 53.51±2.45%.

[0063] Example 6 Picking ripe Cymbidium pods: According to local climatic conditions, during the Frost Descent period (October 20th to October 25th) each year, choose healthy Cymbidium pods and pick them as a whole from the base of the pods. Be careful to avoid damaging the pods during the picking process, and remove shriveled, blackened, and worm-eyed Cymbidium pods.

[0064] Shade drying of Cymbidium orchid pods: Place the picked Cymbidium orchid pods on a drying tray in a dry and ventilated area, and dry them in the shade at room temperature until the moisture content of the seeds is less than 10%. Avoid direct sunlight during the drying process.

[0065] Removing the cymbidium fruit pods to obtain the cymbidium seeds to be stored: After the cymbidium fruit pods are dried, the skin is mechanically broken, the dried fruit pods are peeled open, the seeds are taken out, and the seeds are sieved out with a 40-mesh sieve to obtain the cymbidium seeds to be stored.

[0066] Sand and sterilization treatment: Take river sand that has passed through a 10-mesh fine sieve and mix it with substrate soil in a volume ratio of 2:1. During the mixing process, spray a 1500-fold diluted solution of 30% carbendazim with a mass concentration for sterilization and insecticide treatment to obtain sterilized sand.

[0067] Mix the sterilized sand and the Cymbidium seeds to be stored: Take the Cymbidium seeds to be stored, mix them evenly with the sterilized sand in a volume ratio of 1:5, and then place the mixture of sand and Cymbidium seeds in a storage box. At the same time, use a 1:1 volume ratio of 30% carbendazim 1500 times diluted solution and a 2.5% mass concentration of chlorpyrifos 800 times diluted solution to mix the sterilization liquid spray, and keep the humidity of the mixture of sand and Cymbidium seeds at 70% to 75%. Regulate the storage temperature: Alternate between 4℃ and 25℃, exchange every 7 days, and after a total of 60 days of treatment, store at 4℃ until sowing. Management of the storage process: Insert the thermometer and hygrometer 3cm above the upper surface of the bottom layer of sand to monitor the temperature and humidity.

[0068] The cymbidium pods were picked during the frost period, and the vitality of the cymbidium seeds was tested to be 77.4±1.2%. The vitality of the cymbidium seeds to be stored after drying in the shade and sieving was 63.25±3.07%. The storage method provided in Example 6 was used to store the cymbidium seeds for 120 days, and the vitality of the cymbidium seeds after storage was tested to be 52.70±3.08%.

[0069] 2. Experimental Test On the basis of Examples 1 to 7, the activity test of the seeds stored by the method provided by the present invention was carried out by TTC (2,3,5-triphenyltetrazolium chloride) staining. The specific method is: take an appropriate amount of seeds to be tested and put them into a centrifuge tube, add a calcium hypochlorite solution with a mass concentration of 5% and a Tween 80 with a volume concentration of 0.8%, treat for 30 minutes to soften the seed coat; rinse with distilled water, soak in water at room temperature for 24 hours; discard the distilled water, add a TTC solution with a mass concentration of 1% to soak, and culture in the dark at 30°C for 24 hours; discard the TTC solution, wash with distilled water to make a seed suspension, and examine it under a stereo microscope. Seeds whose embryos turn orange-red to red are viable seeds.

[0070] See attached Figure 1 This experiment investigates the comparison of seed vitality when collecting Cymbidium orchid seeds at different times. Figure 1 The data shows that Figure 1In the figure, ae are the TTC staining results of fresh seeds picked during the Autumnal Equinox, Cold Dew, Frost Descent, Beginning of Winter and Light Snow periods, respectively; f is the statistical result. The vitality of the seeds sampled during the Autumnal Equinox is 97.8±0.9%, the vitality of the seeds sampled during the Cold Dew period is 84.3±1.5%, the vitality of the seeds sampled during the Frost Descent period is 77.4±1.2%, the vitality of the seeds sampled during the Beginning of Winter is 43.5±1.0%, and the vitality of the seeds sampled during the Light Snow period is 38.1±1.5%. It can be seen that the vitality of the seeds sampled during the Autumnal Equinox is the highest. As the period goes by, the seed vitality gradually decreases. The seed vitality during the Cold Dew period, Frost Descent period, Beginning of Winter period and Light Snow period decreases in sequence. Therefore, the present invention determines that the best harvesting time of Cymbidium seeds is the Autumnal Equinox period.

[0071] See attached Figure 2 , the results of seed vigor detection during the process of storing Cymbidium orchid seeds by the method of the present invention. The vigor of fresh seeds just harvested during the autumnal equinox is 96.28±1.80%, and the vigor of Cymbidium orchid seeds after drying in the shade gradually decreases to 85.44±2.81%. The seed vigor after 30 days of storage according to the storage method of Cymbidium orchid seeds provided in Example 1 of the present invention is 78.25±2.69%, the seed vigor after 60 days of storage is 73.65±2.66%, the seed vigor after 90 days of storage is 72.19±1.76%, and the seed vigor after 120 days of storage is 70.71±1.90%; these data show that the storage method of Cymbidium orchid seeds provided by the present invention can be used to store Cymbidium orchid seeds. The stored Cymbidium seeds can effectively maintain the vitality of the seeds for a long period of time (at least 120 days); the control group: at room temperature, without any treatment, the seed vitality after 30 days was 69.81±2.96%, the seed vitality after 60 days was 48.31±1.55%, the seed vitality after 90 days was 23.89±1.07%, and the seed vitality after 120 days was 8.70±1.35%. It can be seen that with the existing traditional storage method of Cymbidium without treatment, the vitality of Cymbidium seeds decreases rapidly, especially after 90 days and 120 days, the seed vitality is almost lost.

[0072] In summary, through comparison, it can be seen that the storage method of Cymbidium orchid seeds provided by the present invention can effectively maintain the vitality of the seeds, especially after storage for a long time. Compared with the seeds without any treatment at room temperature, the vitality of the seeds stored by the method of the present invention is significantly higher.

[0073] Comparative Example: The vitality of fresh Cymbidium orchid seeds was tested to be 96.28±1.80% when picked during the autumnal equinox. After 120 days of storage at room temperature using the traditional storage method without any treatment, the seed vitality dropped significantly to 8.99±4.03%. The storage method of Cymbidium orchid seeds provided in Example 1 was used to store the seeds for different storage times to test the seed vitality. For details, see the attached Figure 3 shown.

[0074] By the attached Figure 3 The data shows that Figure 3 In the figure, a is the TTC staining result of fresh seeds, and the seed vitality is 96.28±1.80%. The seeds are stored at room temperature using the traditional storage method without any treatment. b is the TTC staining result of seeds after 120 days of storage, and the seed vitality significantly decreases to 8.99±4.03%. The seeds are stored using the storage method of Cymbidium orchid seeds provided in Example 1. After 14 days of storage, the seed vitality significantly recovers to 18.16±2.29%, after 28 days of storage, the seed vitality significantly recovers to 33.43±3.11%, and after 42 days of storage, the seed vitality significantly recovers to 51.56±2.55%; c is the TTC staining result of seeds after 56 days of storage, and the vitality recovers to 53.60±2.77%.

[0075] The above contents are only for explaining the technical idea of ​​the present invention and cannot be used to limit the protection scope of the present invention. Any changes made on the basis of the technical solution in accordance with the technical idea proposed by the present invention shall fall within the protection scope of the claims of the present invention.

Claims

1. A method for storing cymbidium orchid seeds, characterized in that: The steps include: Step 1: Mix the sterilized sandy soil with the Cymbidium orchid seeds to be stored to obtain a mixture of sandy soil and Cymbidium orchid seeds; Step 2: Place the mixture of sand and Cymbidium orchid seeds in a storage box, sterilize and moisturize, adjust the storage temperature, and store it until before sowing to obtain the stored Cymbidium orchid seeds; The storage temperature is regulated as follows: treating alternately in two temperature ranges of 2°C-6°C and 20-30°C, changing the temperature range every 5-9 days, treating for 60 days in total, and then storing at 2°C-6°C until before sowing.

2. The storage method of cymbidium orchid seeds according to claim 1, characterized in that: In step 1, the Cymbidium orchid seeds to be stored are obtained by collecting mature Cymbidium orchid pods, drying them in the shade until the moisture content of the seeds is less than 10%, and sieving them.

3. The storage method of cymbidium orchid seeds according to claim 2, characterized in that: The time for collecting mature Cymbidium orchid pods is any period among the Autumnal Equinox period, Cold Dew period, Frost Descent period and Light Snow period.

4. The storage method of Cymbidium orchid seeds according to claim 1, characterized in that: In step 1, the sterilized sandy soil is obtained by mixing river sand and matrix soil and then sterilizing them; the volume ratio of the river sand and matrix soil is 1:1-3, the river sand is river sand that has passed through a 6-10 mesh fine sieve, and the matrix soil is matrix soil that has passed through a 6-10 mesh fine sieve.

5. The storage method of Cymbidium orchid seeds according to claim 4, characterized in that: The sterilization is specifically carried out by spraying a 1500-fold diluted solution of carbendazim with a mass concentration of 30%.

6. The storage method of Cymbidium orchid seeds according to claim 1, characterized in that: In step 1, the volume ratio of the sterilized sand to the Cymbidium seeds to be stored is 3 to 5:

1.

7. The storage method of Cymbidium orchid seeds according to claim 1, characterized in that: In step 2, the sterilization and humidity maintenance are specifically as follows: spraying a mixed sterilization liquid to make the humidity of the mixture of sand and Cymbidium seeds 70% to 75%, wherein the mixed sterilization liquid is a 1500-fold diluted solution of carbendazim and an 800-fold diluted solution of chloramphenicol in a volume ratio of 1:

1.

8. The storage method of Cymbidium orchid seeds according to claim 1, characterized in that: The storage method further includes monitoring the temperature and humidity of the storage environment, specifically: inserting a thermometer and a hygrometer to a position 3 cm above the upper surface of the bottom layer of sand for monitoring.

9. Application of the storage method for cymbidium seeds as described in any one of claims 1 to 8 in artificial cultivation of cymbidium seedlings.

10. Use of the storage method for cymbidium orchid seeds according to any one of claims 1 to 8 in the storage of orchidaceae plant seeds.

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