Application of native DHA raw milk in improving variety and content of phospholipid DHA of cow milk and cow milk rich in phospholipid DHA

Abstract

The invention provides an application of native DHA raw milk in improving the variety and content of phospholipid DHA in cow milk and cow milk rich in phospholipid DHA. The adopted native DHA raw milk is natural raw milk containing DHA, the phospholipid type DHA of the raw milk is various, the raw milk contains special phosphatidylinositol type DHA (PI-DHA) and cardiolipin type DHA (CLs-DHA), the proportion of phosphatidylserine type DHA (PS-DHA) is obviously higher than that of fish oil and algae oil source DHA milk, the proportion of total phospholipid type DHA in total DHA is obviously higher than that of other fish oil and algae oil DHA milk, and the DHA milk is rich in DHA, rich in DHA and rich in DHA. By adopting the original DHA raw milk to prepare cow milk, the variety and content of phospholipid type DHA of the cow milk can be increased.

Description

Technical Field

[0001] The invention relates to application of native DHA raw milk in increasing the type and content of phospholipid DHA in cow's milk and cow's milk rich in phospholipid DHA, belonging to the technical field of milk preparation. Background Art

[0002] DHA stands for docosahexaenoic acid, which is a ω-3 long-chain polyunsaturated fatty acid. It is mainly enriched in fish oil and algae in nature. DHA is one of the most abundant unsaturated fatty acids in the brain and retinal photoreceptors, and is a nutrient that is very necessary for the development of the brain and optic nerves. DHA is the most abundant essential fatty acid in the brain, and 97% of the ω-3 fatty acids in the brain are DHA. Compared with other fatty acids, the brain will choose to take in DHA first, so the iteration rate of DHA in the brain is very fast. There is a pathway in the human body that uses α-linolenic acid as a precursor to synthesize DHA, but the amount of DHA synthesized through this pathway is extremely small and cannot meet normal physiological needs. Therefore, the human body needs to supplement DHA regularly through diet or DHA supplements. Supplementing enough DHA is very important for the development and maintenance of brain function.

[0003] As one of the main components of cell membranes, phospholipids exist in large quantities in human tissue cells and the nervous system. They are important raw materials for human cells, especially brain cells and nerve cells. In a healthy human body, phospholipids account for about 30% of the weight of the brain stem and 73% of fat and liver. At the same time, phospholipids also protect the entire blood circulation system of the human body and have the functional effect of clearing fat and cholesterol from blood vessels. In addition, phospholipids are also an important biologically active substance that plays a very important role in human health.

[0004] Phospholipid DHA refers to DHA contained in the fatty acids that make up phospholipids, also known as DHA-type phospholipids. In addition to the physiological functions of phospholipids, phospholipid DHA has a special fatty acid composition. On the basis of the functional characteristics of phospholipids and DHA, it also has a variety of biological activities and has the dual effects of DHA and phospholipids. The physiological functions of phospholipid DHA include improving the oxidative stability of DHA, facilitating the absorption of DHA, enhancing cell membrane permeability, enhancing the stress resistance of organisms, and anti-inflammatory effects. Summary of the invention

[0005] In order to solve the above technical problems, the purpose of the present invention is to provide a method for increasing the phospholipid DHA in cow's milk, which uses native DHA raw milk as a raw material to prepare cow's milk to increase the type and content of phospholipid DHA.

[0006] In order to achieve the above-mentioned object, the present invention firstly provides the use of native DHA raw milk in increasing the type and content of phospholipid DHA in cow's milk.

[0007] Compared with the common technical solutions in the prior art that use fish oil, algae oil, etc. as raw materials to increase the DHA content of cow's milk by adding them externally, the present invention can significantly increase the type and content of phospholipid DHA in the prepared cow's milk products by using native DHA raw milk.

[0008] According to a specific embodiment of the present invention, preferably, the native DHA raw milk is prepared according to the feeding and management method of dairy cows in CN 105660520B.

[0009] According to a specific embodiment of the present invention, preferably, the native DHA raw milk is used as the raw material for cow's milk.

[0010] According to a specific embodiment of the present invention, preferably, the DHA content of the native DHA raw milk is above 10 mg / 100 ml.

[0011] According to a specific embodiment of the present invention, preferably, the native DHA raw milk contains phospholipid DHA. Compared with the common technical solutions in the prior art that use fish oil, algae oil, etc. as raw materials to increase the DHA content of cow's milk by adding them externally, the present invention can significantly increase the type and content of phospholipid DHA in the prepared cow's milk product by using native DHA raw milk.

[0012] The present invention can provide rich phospholipid DHA to cow's milk by adopting native DHA raw milk, which far exceeds the content that can be provided by fish oil and algae oil.

[0013] According to a specific embodiment of the present invention, preferably, based on the total DHA content of the cow's milk, the content of the phospholipid DHA is 8%-35%; more preferably, it is 20.95%±0.65%.

[0014] According to a specific embodiment of the present invention, preferably, the bovine milk contains one or a combination of two or more of PI-type DHA, CLs-type DHA and PS-type DHA.

[0015] According to a specific embodiment of the present invention, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the PI DHA is 1%-10%; more preferably 3.56%±0.07%.

[0016] According to a specific embodiment of the present invention, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the CLs DHA is 10%-20%; more preferably, 14.17%±0.10%.

[0017] According to a specific embodiment of the present invention, preferably, based on the total phospholipid DHA content of the milk, the content of the PS DHA is 10%-20%; more preferably, 15.43%±0.02%.

[0018] According to a specific embodiment of the present invention, preferably, the bovine milk contains one or a combination of two or more of PE-type DHA, PC-type DHA and LPLs-type DHA.

[0019] According to a specific embodiment of the present invention, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the PE DHA is 40%-50%; more preferably, 46.79%±0.18%.

[0020] According to a specific embodiment of the present invention, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the PC-type DHA is 5%-20%; more preferably, it is 12.86%±0.03%.

[0021] According to a specific embodiment of the present invention, preferably, based on the total phospholipid DHA content of the bovine milk, the content of the LPLs DHA is 5.5%-7.5%; more preferably 7.19%±0.07%.

[0022] According to a specific embodiment of the present invention, preferably, based on the total phospholipid DHA content of the milk, the milk contains: PI (18:0 / 22:6) 3.56%, PE (14:0 / 22:6) 3.92%, PE (15:0 / 22:6) 3.37%, PE (18:1e / 22:6) 3.73%, PE (18:2e / 22:6) 3.61%, PS (16:0 / 2 2:6)3.59%, PS(18:0 / 22:6)4.45%, PS(20:3 / 22:6)3.76%, LPE(22:6)3.63%, CL(21:0 / 18 :0 / 18:0 / 22:6)3.74%, CL(22:6 / 15:0 / 18:0 / 20:0)3.96%, MLCL(14:2 / 22:6 / 22:6)6.47%.

[0023] The present invention also provides cow's milk rich in phospholipid DHA, wherein the cow's milk is prepared using native DHA raw milk as a raw material; the native DHA raw milk is prepared according to the cow feeding and management method in CN 105660520 B.

[0024] In the above-mentioned cow's milk, preferably, the DHA content of the cow's milk is above 10 mg / 100 ml.

[0025] In the above-mentioned cow's milk, preferably, the native DHA raw milk used contains phospholipid DHA.

[0026] In the above-mentioned cow's milk, preferably, based on the total DHA content of the cow's milk, the content of the phospholipid DHA is 8%-35%; more preferably, 20.95%±0.65%.

[0027] In the above-mentioned cow's milk, preferably, the cow's milk contains one or a combination of two or more of PI-type DHA, CLs-type DHA and PS-type DHA.

[0028] In the above-mentioned cow's milk, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the PI DHA is 1%-10%; more preferably, 3.56%±0.07%.

[0029] In the above-mentioned cow's milk, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the CLs-type DHA is 10%-20%; more preferably, 14.17%±0.10%.

[0030] In the above-mentioned cow's milk, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the PS-type DHA is 10%-20%; more preferably, 15.43%±0.02%.

[0031] In the above-mentioned cow's milk, preferably, the cow's milk contains one or a combination of two or more of PE-type DHA, PC-type DHA and LPLs-type DHA.

[0032] In the above-mentioned cow's milk, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the PE DHA is 40%-50%; more preferably, 46.79%±0.18%.

[0033] In the above-mentioned cow's milk, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the PC-type DHA is 5%-20%; more preferably, 12.86%±0.03%.

[0034] In the above-mentioned cow's milk, preferably, based on the total phospholipid DHA content of the cow's milk, the content of the LPLs DHA is 5.5%-7.5%; more preferably, 7.19%±0.07%.

[0035] In the above-mentioned milk, preferably, based on the total phospholipid DHA content of the milk, the milk contains: PI (18:0 / 22:6) 3.56%, PE (14:0 / 22:6) 3.92%, PE (15:0 / 22:6) 3.37%, PE (18:1e / 22:6) 3.73%, PE (18:2e / 22:6) 3.61%, PS (16:0 / 22:6 )3.59%, PS(18:0 / 22:6)4.45%, PS(20:3 / 22:6)3.76%, LPE(22:6)3.63%, CL(21:0 / 18:0 / 18:0 / 22:6)3.74%, CL(22:6 / 15:0 / 18:0 / 20:0)3.96%, MLCL(14:2 / 22:6 / 22:6)6.47%.

[0036] The cow's milk rich in phospholipid DHA provided by the present invention can be prepared by using a pure milk preparation process commonly used in the art, for example, according to the following process steps:

[0037] 1) Collection and storage of DHA-rich raw milk: Collect milk according to raw milk standards, with a collection temperature of 1-8°C. After collecting the milk, filter it with a 60-80 mesh single or double filter, and then cool it to 1-6°C for storage.

[0038] 2) Milk separation: The milk in step 1) is sterilized and impurities removed by a high-speed centrifuge, with a separation temperature of 50-65° C. and a separation speed of 4500-5000 rpm / min.

[0039] 3) Pre-sterilization: homogenize and pre-sterilize the sterilized and impurity-removed milk in step 2) at a homogenization temperature of 50-65°C and a homogenization pressure of 160-200 bar for primary homogenization and 20-40 bar for secondary homogenization; pre-sterilization requires heating the milk to 75-85°C, keeping it warm for 10-25s (preferably 15s), and then cooling it to 1-8°C for storage.

[0040] 4) Ultra-high temperature sterilization: vacuum degas the milk in step 3), then heat the milk to 70-75° C. and homogenize it at a homogenization pressure of 200-300 bar for primary homogenization and 40-60 bar for secondary homogenization; heat the homogenized milk to 137-140° C. and keep it for 3-8 seconds (preferably 4 seconds), then cool it to 20-30° C. to obtain sterilized milk, i.e., native DHA milk.

[0041] 5) Filling: The sterilized milk is filled and stored in an ultra-clean or aseptic environment.

[0042] The native DHA raw milk used in the present invention is raw milk that naturally contains DHA. The raw milk contains various types of phospholipid DHA, such as phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylcholine (PC), lysophosphatide (LPL), and cardiolipin (CL). In addition, phosphatidylinositol-type DHA and cardiolipin-type DHA are only detected in native DHA milk and are not detected in exogenously fortified DHA milk. Using the native DHA raw milk to prepare cow's milk can increase the types and content of phospholipid DHA in cow's milk. DETAILED DESCRIPTION

[0043] In order to have a clearer understanding of the technical features, purposes and beneficial effects of the present invention, the technical solution of the present invention is now described in detail below, but it should not be construed as limiting the applicable scope of the present invention.

[0044] Example 1 Native DHA milk

[0045] The present embodiment provides a native DHA milk, the raw material of which contains natural DHA-rich raw milk obtained through feed regulation technology. The preparation method of the DHA-rich raw milk refers to Example 2 of patent CN201410673240.8. The raw milk naturally and stably contains DHA, and the content is above 10 mg / 100 ml.

[0046] The process of producing native DHA milk using DHA-rich raw milk as raw material is as follows:

[0047] 1) Collection and storage of DHA-rich raw milk: Collect milk according to raw milk standards, with a collection temperature of 1-8°C. After collecting the milk, filter it with a 60-80 mesh single or double filter, and then cool it to 1-6°C for storage.

[0048] 2) Milk separation: The milk in step 1) is sterilized and impurities removed by a high-speed centrifuge, with a separation temperature of 50-65° C. and a separation speed of 4500-5000 rpm / min.

[0049] 3) Pre-sterilization: homogenize and pre-sterilize the sterilized and impurity-free milk in step 2) at a homogenization temperature of 50-65°C and a two-stage homogenization of 30 / 180±5bar (first adjust the second stage so that the pressure gauge indicates 30bar, and then adjust the first stage so that the pressure gauge indicates 180±5bar); pre-sterilization requires heating the milk to 80°C, keeping it warm for 15s, and then cooling it to 1-8°C for storage.

[0050] 4) Ultra-high temperature sterilization: vacuum degas the milk in step 3), then heat the milk to 70-75°C and homogenize it at a homogenization pressure of 250±5 bar for the first homogenization and 50 bar for the second homogenization; heat the homogenized milk to 137-140°C and keep it for 4 seconds, then cool it to 20-30°C to obtain sterilized milk, i.e. native DHA milk.

[0051] 5) Filling: The sterilized milk is filled and stored in an ultra-clean or aseptic environment.

[0052] Comparative Example 1 Algae Oil DHA Emulsion

[0053] This comparative example provides an algae oil DHA milk, which is prepared by adding algae oil raw material to pure milk without DHA and processing through a conventional homogenization process, and the process is as follows:

[0054] 1) Ordinary raw milk collection and storage: Collect milk according to raw milk standards, the collection temperature is 1-8℃, after collecting the milk, use a 60-80 mesh single or double filter to filter, and then cool to 1-6℃ for storage.

[0055] 2) Milk separation: The milk in step 1) is sterilized and impurities removed by a high-speed centrifuge, with a separation temperature of 50-65° C. and a separation speed of 4500-5000 rpm / min.

[0056] 3) Mixing and pre-sterilization: The sterilized and impurity-free milk in step 2) is homogenized and pre-sterilized at a homogenization temperature of 55°C and a secondary homogenizer of 30 / 180 bar. The milk is then heated to 75°C, kept warm for 15 seconds, and then cooled to 1-8°C for storage.

[0057] 4) Ingredients: Heat the milk in step 3) accounting for 10-15% of the ingredients to 50°C, add 0.057% algae oil (DHA ≥ 350 mg / g), use a mixing, dispersing and shearing equipment to mix the ingredients for 10-15 minutes, and then homogenize at a homogenization pressure of 30 / 180 bar. After homogenization, use the remaining milk in step 3) to make the volume 100% and cool to 1-8°C.

[0058] 5) Ultra-high temperature sterilization: vacuum degas the milk in step 4), then heat the milk to 70-75°C and homogenize it at a pressure of 50 / 250±5bar; heat the homogenized milk to 137-140°C and maintain for 4 seconds, then cool it to 20-30°C to obtain sterilized milk, i.e., algae oil DHA milk.

[0059] 6) Filling: The sterilized milk is filled and stored in an ultra-clean or aseptic environment.

[0060] Comparative Example 2 Fish Oil DHA Emulsion

[0061] This comparative example provides a fish oil DHA milk, which is prepared by adding fish oil raw materials to pure milk without DHA and processing through a conventional homogenization process, and the process is as follows:

[0062] 1) Ordinary raw milk collection and storage: Collect milk according to raw milk standards, the collection temperature is 1-8℃, after collecting the milk, use a 60-80 mesh single or double filter to filter, and then cool to 1-6℃ for storage.

[0063] 2) Milk separation: The milk in step 1) is sterilized and impurities removed by a high-speed centrifuge, with a separation temperature of 50-65° C. and a separation speed of 4500-5000 rpm / min.

[0064] 3) Mixing and pre-sterilization: homogenize and pre-sterilize the sterilized and impurity-free milk in step 2) at a homogenization temperature of 55°C and a secondary homogenizer of 30 / 180 bar; then heat the milk to 75°C, keep it warm for 15 seconds, and then cool it to 1-8°C for storage.

[0065] 4) Ingredients: Heat the milk in step 3) accounting for 10-15% of the ingredients to 50°C, add 0.16% of fish oil (DHA ≥ 125 mg / g), use a mixing, dispersing and shearing device to mix the ingredients for 10-15 minutes, and then homogenize at a homogenization pressure of 30 / 180 bar. After homogenization, use the remaining milk in step 3) to make the volume 100%, and cool to 1-8°C.

[0066] 5) Ultra-high temperature sterilization: vacuum degas the milk in step 4), then heat the milk to 70-75°C and homogenize it at a pressure of 50 / 250±5bar; heat the homogenized milk to 137-140°C and keep it for 4s, then cool it to 20-30°C to obtain sterilized milk, i.e., fish oil DHA milk.

[0067] 5) Filling: The sterilized milk is filled and stored in an ultra-clean or aseptic environment.

[0068] Experimental test:

[0069] 1. Total DHA content in the sample

[0070] The test results of total DHA content in different samples are shown in Table 1.

[0071] Table 1 Total DHA content in samples

[0072] Sample name Total DHA content Native DHA milk 0.214mg / mL Algae Oil DHA Milk 0.170mg / mL Fish Oil DHA Milk 0.211mg / mL

[0073] 2. Sample lipidomics test

[0074] After freeze-drying the milk sample, weigh about 0.4g of freeze-dried powder in a 15mL centrifuge tube, add 8mL of dichloromethane-methanol solution, vortex and centrifuge at 9000r / min for 5min, and take the supernatant. Add 4mL of dichloromethane-petroleum ether solution to the precipitate, vortex and centrifuge at 9000r / min for 5min, take the supernatant, repeat twice, combine the supernatants, blow dry with nitrogen, and obtain the lipid components in milk.

[0075] The prepared lipid solution was injected into the LC-MS analysis, and the analysis conditions were as follows: chromatographic column (BEH-C18, 1.6μm, 2.1×100mm), the mobile phase was a solution prepared by mixing water, acetonitrile and isopropanol, wherein phase A: water: acetonitrile: isopropanol = 5:3:2, containing 10mM ammonium formate, phase B: water: acetonitrile: isopropanol = 1:9:90, containing 10mM ammonium formate. The initial mobile phase ratio was 10% phase B, at 1min, the phase B ratio increased to 40%, at 3min, the phase B ratio increased to 75%, to 6min, the phase B ratio increased to 100%, maintained for 1min; then the phase B ratio was gradually reduced, at 7min, the phase B was restored to 10%, and the total running time was 10min. The mass spectrometry ion source was an electrospray ion source, positive ion mode, capillary voltage 4.2KV, and ion source temperature 350℃. The mass spectrometry acquisition adopted the dd-MS2 mode, and the loop count was set to 5.

[0076] 3. Data statistics

[0077] Lipidomics analysis software was used to process the collected data, identify the lipid types in each sample, screen out DHA-related lipids for analysis, and use the area normalization method to calculate the proportion of each component in a specific lipid classification.

[0078] 4. Experimental results

[0079] 4.1 Analysis of the proportion of different forms of DHA in samples

[0080] The proportion (%) of different types of DHA in the samples is shown in Table 2.

[0081] Table 2 Proportion of different types of DHA in samples (%)

[0082]

[0083] Note: Data are expressed as mean ± standard deviation (Mean ± SD) (n = 3 for each sample group). English abbreviations: PI-phosphatidylinositol, PE-phosphatidylethanolamine, PG-phosphatidylglycerol, PS-phosphatidylserine, PC-phosphatidylcholine, PA-phosphatidic acid, LPLs-lysophospholipids, CLs-cardiolipins, TG-triglyceride, DG-diaceryl, MG-monoglyceride.

[0084] 4.2. Percentage of phospholipid DHA in samples

[0085] The specific composition of phospholipid DHA in different dairy products is recorded in Table 3.

[0086] Table 3 Composition and relative percentage of phospholipid DHA in different milks (%)

[0087]

[0088]

[0089] Note: Data are expressed as mean ± standard deviation (Mean ± SD) (n = 3 for each sample group). English abbreviations: PI-phosphatidylinositol, PE-phosphatidylethanolamine, PS-phosphatidylserine, PC-phosphatidylcholine, LPL-lysophospholipid, CL-cardiolipin.

[0090] According to the experimental results in Table 1, Table 2 and Table 3, it can be seen that:

[0091] Example 1 and Comparative Examples 1 and 2 of the present invention respectively describe the differences in phospholipid DHA in different DHA source products. The three milk samples only contain phospholipid DHA and glyceride DHA. Among them:

[0092] Glyceride-type DHA is mainly triglyceride-type (accounting for more than 90%), and the rest is diglyceride-type. There is no monoglyceride-type DHA.

[0093] The phospholipid DHA in milk samples mainly exists in the form of phosphatidylethanolamine and phosphatidylcholine.

[0094] Compared with exogenously added DHA fortified milk, the proportion of phosphatidylethanolamine DHA and phosphatidylcholine DHA in native DHA milk has decreased, and the proportion of phosphatidylserine DHA and lysophospholipid DHA has increased instead; in addition, a large amount of phosphatidylinositol DHA and cardiolipin DHA that do not exist in exogenously added DHA fortified milk are also detected in native DHA milk. In native DHA milk, the proportion of phosphatidylserine DHA and cardiolipin DHA is comparable to that of phosphatidylcholine DHA.

[0095] The difference between Example 1 and Comparative Example 1 and Comparative Example 2 is that the sources of DHA are different. The sample phospholipid DHA is detected and analyzed, and it is found that the proportion of phospholipid DHA in the native DHA milk is 20.95%, which is significantly higher than that of algae oil DHA milk (5.76%) and fish oil DHA milk (4.67%). The different types of phospholipid DHA in the sample are analyzed, and it is found that the native DHA milk contains phosphatidylinositol DHA and cardiolipin DHA alone, accounting for 3.56% and 14.17% of the total phospholipid DHA, respectively. These two phospholipid DHAs are not detected in algae oil DHA milk and fish oil DHA milk. The proportion of phosphatidylserine DHA in the native DHA milk is 15.43%, which is 1.8 times that of algae oil DHA milk and 3.3 times that of fish oil DHA milk.

[0096] Comparison of the differences in the subdivision of phospholipid DHA in the samples of Example 1, Comparative Example 1 and Comparative Example 2 revealed that some phospholipid DHA was only detected in native DHA milk, and its proportion of total phospholipid DHA in native DHA milk was PI (18:0 / 22:6) 3.56%, PE (14:0 / 22:6) 3.92%, PE (15:0 / 22:6) 3.37%, PE (18:1e / 22:6) 3.73%, PE (18:2e / 22:6) 3.89%. :6) 3.61%, PS (16:0 / 22:6) 3.59%, PS (18:0 / 22:6) 4.45%, PS (20:3 / 22:6) 3.76%, LPE (22:6) 3.63%, CL (21:0 / 18:0 / 18:0 / 22:6) 3.74%, CL (22:6 / 15:0 / 18:0 / 20:0) 3.96%, MLCL (14:2 / 22:6 / 22:6) 6.47%. PC (18:1 / 22:6) accounts for 4.43% of native DHA milk, which is 4.7 times that of algae oil DHA milk and 5.9 times that of fish oil DHA milk.

[0097] Based on the above content, it can be seen that using raw milk rich in DHA can enable cow's milk to obtain more types and contents of phospholipid DHA.

Claims

1. The application of native DHA raw milk in increasing the types and content of phospholipid DHA in cow's milk.

2. The use according to claim 1, wherein: The native DHA raw milk is prepared according to the feeding and management method of dairy cows in CN 105660520B; The native DHA raw milk is used as the raw material for making cow's milk.

3. The use according to claim 1 or 2, wherein: The DHA content of the native DHA raw milk is above 10 mg / 100 ml.

4. The use according to claim 1, wherein: The native DHA raw milk contains phospholipid DHA; Preferably, based on the total DHA content of the cow's milk, the content of the phospholipid DHA is 8%-35%; more preferably, it is 20.95%±0.65%.

5. The use according to claim 4, wherein: The cow's milk contains one or a combination of two or more of PI-type DHA, CLs-type DHA and PS-type DHA; Preferably, based on the total phospholipid DHA content of the milk, the content of PI DHA is 1%-10%; more preferably 3.56%±0.07%; Preferably, based on the total phospholipid DHA content of the milk, the content of the CLs DHA is 10%-20%; more preferably 14.17%±0.10%; Preferably, based on the total phospholipid DHA content of the milk, the content of the PS DHA is 10%-20%; more preferably, 15.43%±0.02%.

6. The use according to claim 4, wherein: The cow's milk contains one or a combination of two or more of PE-type DHA, PC-type DHA and LPLs-type DHA; Preferably, based on the total phospholipid DHA content of the milk, the content of the PE DHA is 40%-50%; more preferably 46.79%±0.18%; Preferably, based on the total phospholipid DHA content of the milk, the content of the PC DHA is 5%-20%; more preferably 12.86%±0.03%; Preferably, based on the total phospholipid DHA content of the milk, the content of LPLs DHA is 5.5%-7.5%; more preferably, 7.19%±0.07%.

7. The use according to claim 1, wherein: Based on the total phospholipid DHA content of the milk, the milk contains: PI (18:0 / 22:6) 3.56%, PE (14:0 / 22:6) 3.92%, PE (15:0 / 22:6) 3.37%, PE (18:1e / 22:6) 3.73%, PE (18:2e / 22:6) 3.61%, PS (16:0 / 22:6) 3.59 %, PS(18:0 / 22:6)4.45%, PS(20:3 / 22:6)3.76%, LPE(22:6)3.63%, CL(21:0 / 18:0 / 1 8:0 / 22:6)3.74%, CL(22:6 / 15:0 / 18:0 / 20:0)3.96%, MLCL(14:2 / 22:6 / 22:6)6.47%.

8. A cow's milk rich in phospholipid DHA, wherein: The cow's milk is prepared using native DHA raw milk as a raw material; the native DHA raw milk is prepared according to the feeding and management method of dairy cows in CN 105660520 B.

9. The cow's milk according to claim 8, wherein The DHA content of the milk is above 10 mg / 100 ml.

10. The cow's milk according to claim 8, wherein The native DHA raw milk contains phospholipid DHA; Preferably, based on the total DHA content of the cow's milk, the content of the phospholipid DHA is 8%-35%; more preferably, it is 20.95%±0.65%.

11. The cow's milk according to claim 8, wherein The cow's milk contains one or a combination of two or more of PI-type DHA, CLs-type DHA and PS-type DHA; Preferably, based on the total phospholipid DHA content of the milk, the content of PI DHA is 1%-10%; more preferably 3.56%±0.07%; Preferably, based on the total phospholipid DHA content of the milk, the content of the CLs DHA is 10%-20%; more preferably 14.17%±0.10%; Preferably, based on the total phospholipid DHA content of the milk, the content of the PS DHA is 10%-20%; more preferably, 15.43%±0.02%.

12. The cow's milk according to claim 8, wherein The cow's milk contains one or a combination of two or more of PE-type DHA, PC-type DHA and LPLs-type DHA; Preferably, based on the total phospholipid DHA content of the milk, the content of the PE DHA is 40%-50%; more preferably 46.79%±0.18%; Preferably, based on the total phospholipid DHA content of the milk, the content of the PC DHA is 5%-20%; more preferably 12.86%±0.03%; Preferably, based on the total phospholipid DHA content of the milk, the content of LPLs DHA is 5.5%-7.5%; more preferably, 7.19%±0.07%.

13. The cow's milk according to claim 8, wherein Based on the total phospholipid DHA content of the milk, the milk contains: PI (18:0 / 22:6) 3.56%, PE (14:0 / 22:6) 3.92%, PE (15:0 / 22:6) 3.37%, PE (18:1e / 22:6) 3.73%, PE (18:2e / 22:6) 3.61%, PS (16:0 / 22:6) 3.59 %, PS(18:0 / 22:6)4.45%, PS(20:3 / 22:6)3.76%, LPE(22:6)3.63%, CL(21:0 / 18:0 / 1 8:0 / 22:6)3.74%, CL(22:6 / 15:0 / 18:0 / 20:0)3.96%, MLCL(14:2 / 22:6 / 22:6)6.47%.

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