Anti-aging liquid dressing composition containing umbilical cord source cell exosome and preparation method

Abstract

An anti-aging liquid dressing composition containing umbilical cord source cell exosome belongs to the technical field of cosmetics, and is prepared from botryine, lucid ganoderma extract, 17-type collagen, retinol, two-row yeast, butanediol, glycerol, hydrolyzed sodium hyaluronate, EDTA (Ethylene Diamine Tetraacetic Acid) disodium, water, mannitol, trehalose and umbilical cord source cell exosome. The preparation method of the anti-aging liquid dressing composition containing the umbilical cord source cell exosome comprises three steps. The finished product obtained through various raw materials and the corresponding preparation method is suitable for the skin aging problem caused by various reasons, the composition has an innovative multi-dimensional stereoscopic mechanism, and the skin aging problem is fundamentally solved from inside to outside; and by utilizing a fresh technology for storage, higher activity is ensured, the composition is suitable for skin aging caused by various reasons, the skin aging problem is improved, meanwhile, the skin barrier can be repaired, the problems of pigmentation and the like can be improved, and the skin can be kept moist and healthy.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetic preparation, and in particular to an anti-aging liquid dressing composition containing umbilical cord cell exosomes and a preparation method thereof. Background Art

[0002] Skin is the largest organ in the human body and a physical barrier against the external environment. It plays an extremely important role in protecting various organs of the body. Like other body tissues, skin aging is mainly divided into intrinsic aging and extrinsic aging. Intrinsic aging is caused by changes in time, which leads to the accumulation of cell damage, characterized by thinning of the skin and the formation of fine lines; in extrinsic aging, the reduction in skin thickness is largely due to the reduction in cell proliferation and the significant changes in the dermis components caused by aging.

[0003] Specifically, the components of the extracellular matrix (ECM) of human skin (collagen, elastin, glycosaminoglycans, etc.) will significantly decrease with the intrinsic aging of the skin; in addition, with aging, the reactive oxygen species produced by various factors such as mitochondrial damage and inflammatory response increase, and at the same time, the age-related cell repair ability decreases, which increases oxidative stress, leading to the intrinsic aging of the skin. Extrinsic aging is caused by environmental factors, such as air pollution, smoking, malnutrition and ultraviolet (UV) rays. Skin aging will reduce the skin's ability to retain water, causing dryness and itching; the reduction of collagen and elastin will cause the skin to sag and wrinkle; when exposed to ultraviolet rays, it is more likely to have problems such as pigmentation and spots; the skin's ability to repair and regenerate will decrease with aging, and the skin will be more prone to various problems if it is not protected, and in severe cases, it will be infected by bacteria and viruses. Therefore, timely combating skin aging is the top priority of skin care. Summary of the invention

[0004] In order to overcome the drawbacks of the prior art as described in the background technology due to the lack of an effective cosmetic that can slow down skin aging, the present invention provides an anti-aging liquid dressing composition containing umbilical cord cell exosomes, bosera, ganoderma lucidum extract, type 17 collagen, retinol, and saccharomyces cerevisiae as raw materials, and a finished product prepared in combination with corresponding processes. The finished product is suitable for skin aging caused by various reasons, and can repair the skin barrier, improve pigmentation and other problems while improving skin aging problems, so that the skin remains moisturized and healthy, and a preparation method thereof.

[0005] The technical solution adopted by the present invention to solve its technical problem is: An anti-aging liquid dressing composition containing umbilical cord cell exosomes, comprising bosin, ganoderma lucidum extract, type 17 collagen, retinol, saccharomyces cerevisiae, butylene glycol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, trehalose, and umbilical cord cell exosomes; the bosin ratio is 4%-6%, the ganoderma lucidum extract is 2%-4%, the type 17 collagen ratio is 2%-4%, the retinol ratio is 2%-4%, the saccharomyces cerevisiae ratio is 7%-9%, the butylene glycol ratio is 5%-7%, the glycerol ratio is 2%-4%, the hydrolyzed sodium hyaluronate ratio is 0.5%-1.5%, the disodium EDTA ratio is 0.15%-0.4%, the water ratio is 50%-70%, the mannitol ratio is 5%-7%, and the trehalose ratio is 0.5%-1.5%. , the proportion of umbilical cord cell exosomes is 2%-4%; the preparation method of the anti-aging liquid dressing composition containing umbilical cord cell exosomes comprises the following steps: S1: adding botoxine, ganoderma lucidum extract, type 17 collagen, retinol, and saccharomyces cerevisiae into a beaker in proportion, placing the beaker in a water bath, and then stirring the raw materials in the beaker with an electric stirring device until they are dissolved and transparent to prepare a phase A finished product; S2: mixing butanediol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, trehalose, and umbilical cord cell exosomes in proportion and dispensing them into 5 ml ampoules, and placing the ampoules in a freeze dryer to prepare freeze-dried powder to obtain a phase B finished product; S3: packaging the phase A finished product and the phase B finished product separately. In application, the phase A finished product and the phase B finished product are mixed in a ratio of 10-20:0.5-1.5, respectively, and then applied to the skin.

[0006] Furthermore, the ratio of bosacein is 5%, the ratio of ganoderma lucidum extract is 3%, the ratio of type 17 collagen is 3%, the ratio of retinol is 3%, the ratio of saccharomyces cerevisiae is 8%, the ratio of butylene glycol is 6%, the ratio of glycerol is 3%, the ratio of hydrolyzed sodium hyaluronate is 1%, the ratio of disodium EDTA is 0.3%, the ratio of water is 57.7%, the ratio of mannitol is 6%, the ratio of trehalose is 1%, and the ratio of umbilical cord cell exosomes is 3%.

[0007] Furthermore, the temperature of the water bath is 75-85°C, preferably 80°C.

[0008] Furthermore, the stirring rod of the electric stirring device has a rotation speed of 500-600 revolutions per minute, preferably 520 revolutions per minute.

[0009] Compared with the prior art, the present invention has the following beneficial effects: the finished product obtained by the present invention through multiple raw materials and corresponding preparation methods is suitable for skin aging problems caused by various reasons, and the composition has an innovative multi-dimensional three-dimensional mechanism, which fundamentally solves the problem of skin aging from the inside out; it is stored by fresh technology to ensure higher activity, and is suitable for skin aging caused by various reasons. While improving skin aging problems, it can also repair the skin barrier, improve pigmentation and other problems, and keep the skin hydrated and healthy.

[0010] Figure content Figure 1 It is a schematic diagram of the process of preparing the anti-aging liquid dressing composition containing umbilical cord-derived cell exosomes of the present invention. DETAILED DESCRIPTION

[0011] Figure 1 As shown, the anti-aging liquid dressing composition containing umbilical cord cell exosomes includes bosaiin, ganoderma lucidum extract, type 17 collagen, retinol, bismuth yeast, butylene glycol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, trehalose, and umbilical cord cell exosomes. In Example 1, the proportion of bosaiin is 6%, the proportion of ganoderma lucidum extract is 2%, the proportion of type 17 collagen is 4%, the proportion of retinol is 2%, the proportion of bismuth yeast is 9%, the proportion of butylene glycol is 5%, the proportion of glycerol is 4%, the proportion of hydrolyzed sodium hyaluronate is 0.5%, the proportion of disodium EDTA is 0.4%, the proportion of water is 54.6%, the proportion of mannitol is 7%, the proportion of trehalose is 1.5%, and the proportion of umbilical cord cell exosomes is 4%. In Example 2, the ratio of Bossain is 4%, the ratio of Ganoderma lucidum extract is 4%, the ratio of type 17 collagen is 2%, the ratio of retinol is 4%, the ratio of bisect yeast is 7%, the ratio of butylene glycol is 7%, the ratio of glycerol is 2%, the ratio of hydrolyzed sodium hyaluronate is 1.5%, the ratio of disodium EDTA is 0.15%, the ratio of water is 60.85%, the ratio of mannitol is 5%, the ratio of trehalose is 0.5%, and the ratio of umbilical cord cell exosomes is 2%. In Example 3, the ratio of Bossain is 5%, the ratio of Ganoderma lucidum extract is 3%, the ratio of type 17 collagen is 3%, the ratio of retinol is 3%, the ratio of bisect yeast is 8%, the ratio of butylene glycol is 6%, the ratio of glycerol is 3%, the ratio of hydrolyzed sodium hyaluronate is 1%, the ratio of disodium EDTA is 0.2%, the ratio of water is 57.8%, the ratio of mannitol is 6%, the ratio of trehalose is 1%, and the ratio of umbilical cord cell exosomes is 3%.

[0012] Figure 1As shown, the preparation method of the anti-aging liquid dressing composition containing umbilical cord cell exosomes comprises the following steps: S1: adding bosaicin, ganoderma lucidum extract, type 17 collagen, retinol, and saccharomyces cerevisiae to a beaker in proportion, placing the beaker in a water bath, and then stirring the raw materials in the beaker by an electric stirring device until they are dissolved and transparent to prepare a phase A finished product; the temperature of the water bath is 75-85°C, preferably 80°C. S2: mixing butanediol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, trehalose, and umbilical cord cell exosomes in proportion and dispensing them into 5 ml ampoules, and placing the ampoules in a freeze dryer to prepare freeze-dried powder to obtain a phase B finished product; the stirring rod of the electric stirring device rotates at a speed of 500-600 revolutions per minute, preferably 520 revolutions. S3: Package the finished products of phase A and phase B separately. During application, mix the finished products of phase A and phase B in a ratio of 10-20:0.5-1.5 respectively, and then apply them on the skin.

[0013] Figure 1As shown, in the present invention, the functions of each raw material are as follows. (1) Umbilical cord cell exosomes: Umbilical cord cell exosomes are bilayer phospholipid vesicles with a diameter of 30-150 nm secreted by umbilical cord stem cells, which contain a variety of miRNAs, amino acids, lipids and cell regulatory factors; studies have found that exosomes can transmit biological information within and between cells, and play an important role in regulating receptor cells. Stem cell exosomes can significantly reduce the expression level of β-galactosidase related to aging and upregulate the expression of collagen in HDFs; exosomes have better transdermal properties, so the nutrients they carry can be absorbed faster and better. can be absorbed and utilized by the skin; Exosomes can promote the regeneration of fibroblasts and collagen synthesis, repair cell aging problems from the bottom of the skin, reduce the formation of wrinkles, maintain skin elasticity, and make the skin have a natural plumpness; Exosomes can also regulate the activity of their own original stem cells, and fundamentally care for and repair the skin from the inside out; Exosomes can regulate the dynamic balance of the skin, improve facial circulation, promote skin metabolism, reduce pigmentation, make the skin more radiant, comprehensively condition the skin, improve damaged skin quality, and make the skin reach a younger and healthier state. (2) Bosogenin: Bosogenin is a natural glycoprotein mixture derived from beech. It can promote the conversion and construction of protein sugar content in proteoglycans. This molecular structure can absorb water and make the culture matrix gel-like, thereby improving the firmness of cells and skin, and promoting the synthesis of collagen and elastic fibers in the skin. Collagen has a good affinity with the tissues around the skin, which helps the skin obtain more nutrients, promotes the repair of the skin barrier, and then enhances the skin barrier function, increases the elasticity and firmness of the skin, and achieves the purpose of delaying skin aging. Bosogenin can also stimulate the activity in the extracellular matrix, activate aging cells, improve uneven skin tone, dullness and other problems, make the skin smoother and more delicate, present a healthy luster, and achieve anti-aging effects. (3) Ganoderma: Ganoderma has been used for thousands of years and is known as a "magic medicine" that can prolong life and keep the body young. Ganoderma extracts also contain a variety of bioactive ingredients that can penetrate deep into the skin, such as Ganoderma polysaccharides, Ganoderma triterpenes and Ganoderma peptides. Ganoderma polysaccharides have a good moisturizing effect, which can promote the skin's ability to retain water, so that the skin remains hydrated and plump for a long time, and says goodbye to roughness and dryness; Ganoderma triterpenes have powerful antioxidant and anti-inflammatory effects, which can effectively resist the damage of free radicals to the skin, enhance the skin's barrier function, thereby reducing the occurrence of skin inflammation and making the skin free from sensitivity; Ganoderma peptides can promote the synthesis of skin collagen, thereby enhancing skin elasticity and firmness, and can effectively delay the aging process of the skin, fundamentally reducing the formation of fine lines and wrinkles, and keeping the skin young; Ganoderma extracts can also effectively inhibit the production of melanin, prevent pigmentation, fade spots, brighten the skin tone and other effects.(4) Type 17 collagen: Type 17 collagen is a transmembrane protein that is mainly found in the basement membrane zone of the skin. It plays an important role in maintaining the integrity and health of the skin structure. Type 17 collagen interacts with intracellular keratin and extracellular basement membrane proteins such as laminin-332 and type IV collagen to establish a strong adhesion between epithelial cells and the basement membrane. This adhesion is not only a physical connection, but also a regulator of processes such as cell proliferation, differentiation and migration, thereby maintaining the integrity of the skin structure. By supplementing type 17 collagen, problems such as skin sagging, fine lines and wrinkles can be greatly improved, making the skin smoother, firmer and more elastic. It can also promote skin repair and regeneration, and improve skin damage caused by various external factors. (5) Retinol: Retinol is a derivative of vitamin A, also known as vitamin A1. It is the only drug approved by the US FDA for reversing photoaging. Retinol helps promote cell metabolism, regulate skin oil secretion, reduce the possibility of inflammation, promote the regeneration of skin cells, and is beneficial for removing dead skin cells on the epidermis and improving the condition of pores. Retinol can promote the metabolism of keratinocytes, while inhibiting the activity of melanin catalyzing enzymes, inhibiting the formation of melanin, allowing keratinocytes to fall off with melanin, achieving the effect of whitening and brightening. Retinol can stimulate the skin's own immune function, regulate immune function, increase the regeneration of skin collagen and elastin, strengthen the skin's natural barrier, help improve skin firmness and elasticity, and make the skin glow with health. Retinol can also prevent abnormalities in dermal connective tissue, stimulate the synthesis of skin extracellular matrix proteins, accelerate the formation of new connective tissue, promote the proliferation of skin cells and collagen synthesis, make the skin firmer, remove wrinkles, and inhibit the activity of matrix metalloproteinases, reduce damage to collagen fibers, and prevent photoaging. (6) Diffusion yeast: Diffusion yeast is a fermentation product of bifid yeast. Bifid yeast can enhance the skin's moisturizing ability. It contains a variety of natural moisturizing factors such as lactic acid and hyaluronic acid, which can reduce the loss of water through the skin, increase the water content of the skin, and repair skin damage, thereby reducing the redness, swelling, allergies and other discomforts caused by external environmental stimulation; Bifid yeast also has a strong antioxidant effect, which can inhibit the generation of free radicals and slow down the aging of the skin, thereby preventing the formation of wrinkles and fine lines; Bifid yeast also has a whitening effect, helping to capture free radicals and prevent skin aging; Bifid yeast can also repair DNA damage and enhance the skin's self-repair ability. It can also form a protective film on the skin surface, replenish nutrients to the skin in a timely manner, effectively prevent ultraviolet damage, thereby reducing pigmentation, and achieving the effect of fading spots and brightening; Bifid yeast is also rich in a variety of antioxidants, which can effectively inhibit the generation of free radicals and slow down the aging of the skin.Butylene glycol, glycerin, hydrolyzed sodium hyaluronate, etc. can moisturize, enhance the skin's own defense capabilities, and improve the skin barrier function. Disodium EDTA can stabilize various active ingredients to prevent them from becoming ineffective due to interference from metal ions, thereby ensuring the effectiveness and safety of skin care products. Mannitol has certain antioxidant properties, which can prevent excessive keratinization and inflammatory reactions of the skin, and help reduce skin sensitivity, redness, and swelling caused by external stimuli. Trehalose has excellent cell protection properties and can help repair damaged skin barriers.

[0014] The testing and evaluation methods involved in the present invention are as follows: 1. Experimental subjects Experimental group (the present invention): 30 cases, 15 males and 15 females, aged 25-45 years; Control group (placebo-ordinary essence): 30 cases, 15 males and 15 females, aged 25-45 years old; The gender, age and other data of the two groups were statistically processed and showed no significant difference (P> 0.05), indicating that the two groups were comparable.

[0015] 2. Treatment method: the present invention was applied to the wrinkles of the experimental group, and the common anti-aging essence was applied to the wrinkles of the control group. The skin wrinkle repair time was recorded and the following evaluation was performed, and the occurrence of discomfort reactions was recorded.

[0016] 3. Evaluation criteria: Evaluation was performed based on whether skin wrinkles were improved.

[0017] Recovery: Symptoms and signs disappear by more than 95%.

[0018] Markedly effective: Clinical symptoms were alleviated by 80%-94%.

[0019] Improvement: Clinical symptoms are alleviated by 50%-79%.

[0020] Ineffective: No improvement in clinical manifestations.

[0021] 4. Results: The results are shown in the following table.

[0022]

[0023] The difference analysis between the experimental group and the control group (P < 0.05) showed significant difference, which was statistically significant. The effective rate of the experimental group was significantly higher than that of the control group, indicating that the experimental group had obvious improvement in skin fine lines, and no discomfort occurred in the experimental group, indicating that the present invention had a good effect.

[0024] Figure 1As shown, through the above technical scheme, the present invention combines umbilical cord cell exosomes, bosera, ganoderma lucidum extract, type 17 collagen, retinol, and bisporus yeast, and the finished product obtained by multiple raw materials and corresponding preparation methods is suitable for skin aging problems caused by various reasons. The composition has an innovative multi-dimensional three-dimensional mechanism, which fundamentally solves the problem of skin aging from the inside to the outside; it uses fresh technology for storage to ensure higher activity, and is suitable for skin aging caused by various reasons. While improving skin aging problems, it can also repair the skin barrier, improve pigmentation and other problems, and keep the skin hydrated and healthy.

[0025] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0026] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. An anti-aging liquid dressing composition containing umbilical cord-derived cell exosomes, characterized in that: Including Bossain, Ganoderma lucidum extract, type 17 collagen, retinol, yeast, butylene glycol, glycerin, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, trehalose, and umbilical cord cell exosomes; the Bossain ratio is 4%-6%, the Ganoderma lucidum extract is 2%-4%, the type 17 collagen ratio is 2%-4%, the retinol ratio is 2%-4%, the yeast ratio is 7%-9%, the butylene glycol ratio is 5%-7%, the glycerin ratio is 2%-4%, the hydrolyzed sodium hyaluronate ratio is 0.5%-1.5%, the disodium EDTA ratio is 0.15%-0.4%, the water ratio is 50%-70%, the mannitol ratio is 5%-7%, and the trehalose ratio is 0.5%-1.5% , the proportion of umbilical cord cell exosomes is 2%-4%; the preparation method of the anti-aging liquid dressing composition containing umbilical cord cell exosomes comprises the following steps: S1: adding botoxine, ganoderma lucidum extract, type 17 collagen, retinol, and saccharomyces cerevisiae into a beaker in proportion, placing the beaker in a water bath, and then stirring the raw materials in the beaker with an electric stirring device until they are dissolved and transparent to prepare a phase A finished product; S2: mixing butanediol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, trehalose, and umbilical cord cell exosomes in proportion and dispensing them into 5 ml ampoules, and placing the ampoules in a freeze dryer to prepare freeze-dried powder to obtain a phase B finished product; S3: packaging the phase A finished product and the phase B finished product separately. In application, the phase A finished product and the phase B finished product are mixed in a ratio of 10-20:0.5-1.5, respectively, and then applied to the skin.

2. The anti-aging liquid dressing composition containing umbilical cord cell exosomes according to claim 1, characterized in that: The ratio of bosogenin is 5%, the ratio of ganoderma lucidum extract is 3%, the ratio of type 17 collagen is 3%, the ratio of retinol is 3%, the ratio of saccharomyces cerevisiae is 8%, the ratio of butylene glycol is 6%, the ratio of glycerin is 3%, the ratio of hydrolyzed sodium hyaluronate is 1%, the ratio of disodium EDTA is 0.3%, the ratio of water is 57.7%, the ratio of mannitol is 6%, the ratio of trehalose is 1%, and the ratio of umbilical cord cell exosomes is 3%.

3. The method for preparing the anti-aging liquid dressing composition containing umbilical cord-derived cell exosomes according to claim 1, characterized in that: The temperature of the water bath is 75-85°C, preferably 80°C.

4. The method for preparing the anti-aging liquid dressing composition containing umbilical cord-derived cell exosomes according to claim 1, characterized in that: The stirring rod of the electric stirring device rotates at a speed of 500-600 revolutions per minute, preferably 520 revolutions per minute.

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