Nano donkey-hide gelatin oral liquid and preparation method thereof

Abstract

The invention belongs to the technical field of health-care products, and provides nano donkey-hide gelatin oral liquid and a preparation method thereof. The preparation method comprises the following steps: S1, crushing colla corii asini, mixing the crushed colla corii asini with a surfactant solution, and performing ultrasonic treatment to obtain coated colla corii asini particles; s2, mixing jujubes, Chinese wolfberry fruits and haws, crushing to obtain a mixture, mixing the mixture, the coated donkey-hide gelatin particles and water, and extracting to obtain an extracting solution; s3, concentrating the extracting solution to obtain a concentrated solution; s4, mixing the concentrated solution, psicose and astaxanthin, and performing nanoscale homogenization treatment to obtain the nano donkey-hide gelatin oral liquid. A layer of protective film is formed on the surface of the donkey-hide gelatin fine powder through a surface modification technology, so that the agglomeration phenomenon of the donkey-hide gelatin in the extraction process is effectively prevented. The donkey-hide gelatin particles are further refined through high-pressure homogenization treatment, so that the donkey-hide gelatin particles reach the nanoscale, and the solubility and bioavailability of the donkey-hide gelatin are remarkably improved.

Description

Technical Field

[0001] The invention relates to the technical field of health care products, and in particular to a nano donkey-hide gelatin oral liquid and a preparation method thereof. Background Art

[0002] Donkey-hide gelatin is a traditional Chinese medicine made from the skin of donkeys, an animal of the genus Equidae. Donkey-hide gelatin is an important blood tonic and can be used to treat various blood deficiency syndromes, such as sallow complexion, dizziness, palpitations, etc. It can also be used to treat various bleeding syndromes, such as vomiting blood, epistaxis, bloody stools, metrorrhagia, etc.

[0003] In order to improve the absorption rate of donkey-hide gelatin, the specific surface area of ​​donkey-hide gelatin can be increased. When the size of donkey-hide gelatin particles becomes smaller, its specific surface area increases significantly, and the contact area with the gastrointestinal mucosa increases significantly, so that the effective ingredients in donkey-hide gelatin can more fully contact the digestive juice and intestinal absorption site, thereby accelerating the dissolution rate and improving the absorption efficiency. Small-sized donkey-hide gelatin, especially nano-sized, can more easily cross the gastrointestinal barrier. They can enter the blood circulation through intercellular spaces or by means of cellular endocytosis, improve absorption and distribution in the body, and thus improve the absorption rate. However, after the small-sized donkey-hide gelatin particles have a large specific surface area, the surface atoms or molecules are in an unsaturated state and have a high surface energy. According to the principle of intermolecular forces, these particles tend to approach each other to reduce the surface energy, thereby causing agglomeration. Just like nanomaterials, due to their large specific surface area and high activity, they are extremely unstable and prone to agglomeration. Therefore, how to provide a nano donkey-hide gelatin oral liquid with good absorption rate and not prone to agglomeration has become a problem that needs to be solved by those skilled in the art. Summary of the invention

[0004] In view of this, the present invention provides a nano donkey-hide gelatin oral liquid and a preparation method thereof, the purpose of which is to solve the technical problem that small-particle donkey-hide gelatin is easy to agglomerate, resulting in poor absorption rate.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] The present invention provides a nano donkey-hide gelatin oral liquid, comprising the following raw materials in parts by weight:

[0007] 50-150 parts of donkey-hide gelatin, 10-100 parts of jujube, 10-100 parts of wolfberry, 10-50 parts of hawthorn, 10-50 parts of allulose, 1-10 parts of astaxanthin, and 1-10 parts of surfactant.

[0008] Furthermore, the surfactant includes egg yolk lecithin and / or soybean lecithin.

[0009] The present invention provides a method for preparing the nano donkey-hide gelatin oral liquid, comprising the following steps:

[0010] S1, crushing the donkey-hide gelatin, mixing the crushed donkey-hide gelatin with a surfactant solution, and then subjecting the mixture to ultrasonic treatment to obtain coated donkey-hide gelatin particles;

[0011] S2, mixing jujube, wolfberry and hawthorn and then crushing them to obtain a mixture, mixing the mixture, the coated donkey-hide gelatin particles and water and then extracting to obtain an extract;

[0012] S3, concentrating the extract to obtain a concentrated solution;

[0013] S4. The concentrated solution, allulose and astaxanthin are mixed and then subjected to nano-scale homogenization to obtain nano-donkey-hide gelatin oral solution.

[0014] Furthermore, in step S1, the particle size of the crushed donkey-hide gelatin is 100 to 500 μm.

[0015] Furthermore, in step S1, the mass concentration of the surfactant solution is 0.1-1%.

[0016] Furthermore, in step S1, the ultrasonic treatment time is 10 to 20 minutes.

[0017] Furthermore, in step S2, the particle size of the mixture is 100-500 μm.

[0018] Furthermore, in step S2, the mass ratio of the total mass of the mixture and the coated donkey-hide gelatin particles to water is 1:3-6; the extraction temperature is 60-80°C, and the extraction time is 60-120 min.

[0019] Furthermore, in step S3, the volume ratio of the concentrated solution to the extracted solution is 1:3-5.

[0020] Furthermore, in step S4, the pressure of the nano-scale homogenization treatment is 50-100 MPa, and the number of times of the nano-scale homogenization treatment is 2-3 times.

[0021] It can be seen from the above technical solution that compared with the prior art, the beneficial effects of the present invention are as follows:

[0022] 1. Improve the bioavailability of nutrients: By crushing the donkey-hide gelatin and mixing it with a surfactant solution and then subjecting it to ultrasonic treatment, the coated donkey-hide gelatin particles have a smaller particle size and more uniform dispersion. This treatment method significantly improves the solubility and bioavailability of donkey-hide gelatin, allowing the human body to absorb the nutrients in donkey-hide gelatin more efficiently. Similarly, after jujube, wolfberry and hawthorn are mixed and crushed, their active ingredients can be more fully released during the extraction process, further improving the utilization rate of the overall nutrients.

[0023] 2. Enhance the stability and uniformity of the product: The present invention adopts nano-scale homogenization technology in the preparation process to ensure the uniform distribution of allulose and astaxanthin in the concentrate. This uniform distribution not only improves the stability of the product, but also ensures the consistency of the nutrients in each oral liquid, thereby improving the quality of the product. The use of surfactants not only helps to coat the donkey-hide gelatin particles, but also can prevent the aggregation of nanoparticles to a certain extent, further enhancing the stability of the product.

[0024] 3. Improve the health effects of the product: donkey-hide gelatin, jujube, wolfberry and hawthorn are all traditional Chinese medicinal materials with multiple health effects such as blood tonic, beauty care and anti-oxidation. Through the preparation method of the present invention, the effective ingredients of these ingredients can be more fully extracted and utilized, thereby significantly improving the health effects of the product. Allulose, as a low-calorie sweetener, can not only provide sweetness, but also effectively control blood sugar, and is suitable for use by diabetic patients. Astaxanthin has a strong antioxidant capacity, which can effectively resist free radicals and delay aging.

[0025] 4. Optimize the preparation process and improve production efficiency: The multi-step extraction and concentration process adopted by the present invention can efficiently complete the preparation of nano donkey-hide gelatin oral liquid in a short time, significantly improving production efficiency. The steps are closely connected, the operation is simple, and it is easy to industrialize production, reducing production costs.

[0026] 5. Environmentally friendly and reduces resource waste: In the preparation process, the present invention reduces the waste of raw materials and improves the utilization rate of raw materials by optimizing the extraction and concentration process. At the same time, due to the use of efficient ultrasonic treatment and nano-level homogenization technology, energy consumption is reduced, which conforms to the green and environmentally friendly production concept. DETAILED DESCRIPTION

[0027] The present invention provides a nano donkey-hide gelatin oral liquid, comprising the following raw materials in parts by weight:

[0028] 50-150 parts of donkey-hide gelatin, 10-100 parts of jujube, 10-100 parts of wolfberry, 10-50 parts of hawthorn, 10-50 parts of allulose, 1-10 parts of astaxanthin, and 1-10 parts of surfactant.

[0029] In the present invention, the amount of donkey-hide gelatin used is preferably 80 to 140 parts, and more preferably 100 to 120 parts.

[0030] In the present invention, the amount of jujube used is preferably 20 to 80 parts, more preferably 40 to 60 parts.

[0031] In the present invention, the amount of wolfberry used is preferably 20 to 80 parts, more preferably 40 to 60 parts.

[0032] In the present invention, the amount of hawthorn is preferably 20 to 40 parts, more preferably 30 parts.

[0033] In the present invention, the amount of psicose used is preferably 20 to 40 parts, more preferably 30 parts.

[0034] In the present invention, the amount of astaxanthin used is preferably 2 to 8 parts, more preferably 4 to 6 parts.

[0035] In the present invention, the amount of the surfactant used is preferably 2 to 8 parts, more preferably 4 to 6 parts.

[0036] In the present invention, psicose is prepared by a bioenzymatic method.

[0037] In the present invention, the surfactant includes egg yolk lecithin and / or soybean lecithin.

[0038] The present invention provides a method for preparing the nano donkey-hide gelatin oral liquid, comprising the following steps:

[0039] S1, crushing the donkey-hide gelatin, mixing the crushed donkey-hide gelatin with a surfactant solution, and then subjecting the mixture to ultrasonic treatment to obtain coated donkey-hide gelatin particles;

[0040] S2, mixing jujube, wolfberry and hawthorn and then crushing them to obtain a mixture, mixing the mixture, the coated donkey-hide gelatin particles and water and then extracting to obtain an extract;

[0041] S3, concentrating the extract to obtain a concentrated solution;

[0042] S4. The concentrated solution, allulose and astaxanthin are mixed and then subjected to nano-scale homogenization to obtain nano-donkey-hide gelatin oral solution.

[0043] In the present invention, in step S1, the particle size of the crushed donkey-hide gelatin is 100-500 μm, preferably 200-400 μm, and more preferably 300 μm.

[0044] In the present invention, in step S1, the mass concentration of the surfactant solution is 0.1-1%, preferably 0.2-0.8%, and more preferably 0.4-0.6%.

[0045] In the present invention, in step S1, the ultrasonic treatment time is 10 to 20 minutes, preferably 15 minutes.

[0046] In the present invention, in step S2, the particle size of the mixture is 100 to 500 μm, preferably 200 to 400 μm, and more preferably 300 μm.

[0047] In the present invention, in step S2, the mass ratio of the total mass of the mixture and the coated donkey-hide gelatin particles to water is 1:3-6, preferably 1:4-5; the extraction temperature is 60-80°C, preferably 65-75°C, and more preferably 70°C; the extraction time is 60-120 min, preferably 70-110 min, and more preferably 80-100 min.

[0048] In the present invention, in step S3, the volume ratio of the concentrated solution to the extracted solution is 1:3-5, preferably 1:4.

[0049] In the present invention, in step S4, the pressure of the nano-scale homogenization treatment is 50 to 100 MPa, preferably 60 to 90 MPa, and more preferably 70 to 80 MPa; the number of times of the nano-scale homogenization treatment is 2 to 3 times.

[0050] In the present invention, after the nano-level homogenization treatment, the particle size of the material is 10-100 nm.

[0051] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0052] Example 1

[0053] The raw materials used in this embodiment are as follows:

[0054] 100 parts of donkey-hide gelatin, 70 parts of jujube, 30 parts of wolfberry, 20 parts of hawthorn, 20 parts of allulose, 5 parts of astaxanthin, and 5 parts of soy lecithin.

[0055] The donkey-hide gelatin is crushed into fine powder with a particle size controlled between 100 and 500 μm. The crushed donkey-hide gelatin powder is dispersed in an aqueous solution containing soybean lecithin (mass concentration is 0.5%) and ultrasonically treated for 15 minutes to allow the surfactant to evenly coat the surface of the donkey-hide gelatin particles to form a protective film to prevent agglomeration.

[0056] Jujube, wolfberry and hawthorn are mixed in proportion and crushed into a mixture, and the particle size is controlled between 100 and 500 μm. The mixed powder and the surface-modified donkey-hide gelatin particles are added to the extraction tank, and water 5 times the total mass of the material is added. The extraction is carried out at a constant temperature of 70°C for 100 minutes. After the extraction is completed, the residue is filtered to obtain an extract. The extract is concentrated to 1 / 4 of the original volume, allulose and astaxanthin are added, and stirred evenly. Then, the mixed solution is homogenized twice at 80 MPa by a nano homogenizer to ensure uniform dispersion of nano-scale particles. After homogenization, it is aseptically filled by an aseptic filling machine to obtain a nano donkey-hide gelatin oral solution.

[0057] Example 2

[0058] The raw materials used in this embodiment are as follows:

[0059] 120 parts of donkey-hide gelatin, 50 parts of jujube, 40 parts of wolfberry, 30 parts of hawthorn, 25 parts of allulose, 4 parts of astaxanthin, and 6 parts of soy lecithin.

[0060] The donkey-hide gelatin is crushed into fine powder with a particle size controlled between 100 and 500 μm. The crushed donkey-hide gelatin powder is dispersed in an aqueous solution containing soybean lecithin (mass concentration is 0.6%) and ultrasonically treated for 20 minutes to allow the surfactant to evenly coat the surface of the donkey-hide gelatin particles to form a protective film to prevent agglomeration.

[0061] Jujube, wolfberry and hawthorn are mixed in proportion and crushed into a mixture, and the particle size is controlled between 100 and 500 μm. The mixed powder and the surface-modified donkey-hide gelatin particles are added to an extraction tank, and water 5 times the total mass of the material is added. The extraction is carried out at a constant temperature of 60°C for 120 minutes. After the extraction is completed, the residue is filtered to obtain an extract. The extract is concentrated to 1 / 4 of the original volume, allulose and astaxanthin are added, and stirred evenly. Then, the mixed solution is homogenized 3 times at 70 MPa by a nano homogenizer to ensure uniform dispersion of nano-scale particles. After homogenization, the mixture is aseptically filled by an aseptic filling machine to obtain a nano donkey-hide gelatin oral solution.

[0062] Example 3

[0063] The raw materials used in this embodiment are as follows:

[0064] 150 parts of donkey-hide gelatin, 30 parts of jujube, 30 parts of wolfberry, 30 parts of hawthorn, 30 parts of allulose, 7 parts of astaxanthin, and 3 parts of egg yolk lecithin.

[0065] The donkey-hide gelatin is crushed into fine powder with a particle size controlled between 100 and 500 μm. The crushed donkey-hide gelatin powder is dispersed in an aqueous solution containing soybean lecithin (mass concentration is 0.8%) and ultrasonically treated for 20 minutes to allow the surfactant to evenly coat the surface of the donkey-hide gelatin particles to form a protective film to prevent agglomeration.

[0066] The jujube, wolfberry and hawthorn are mixed in proportion and crushed into a mixture, and the particle size is controlled between 100 and 500 μm. The mixed powder and the surface-modified donkey-hide gelatin particles are added to an extraction tank, and water 5 times the total mass of the material is added. The extraction is carried out at a constant temperature of 80°C for 60 minutes. After the extraction is completed, the residue is removed by filtration to obtain an extract. The extract is concentrated to 1 / 4 of the original volume, allulose and astaxanthin are added, and stirred evenly. Then, the mixed solution is homogenized twice at 100 MPa by a nano homogenizer to ensure uniform dispersion of nano-scale particles. After homogenization, the mixture is aseptically filled by an aseptic filling machine to obtain a nano donkey-hide gelatin oral solution.

[0067] Comparative Example 1

[0068] The same as Example 1, except that no surfactant is used to coat the donkey-hide gelatin.

[0069] Performance Testing

[0070] The blood-tonifying effect of the nano-egg-hide gelatin oral liquid prepared in Examples 1 to 3 and Comparative Example 1 was tested, and the test process was as follows:

[0071] 60 male mice weighing 18-22g and in good health were selected and divided into 6 groups on average, of which 4 groups were drug-administered groups, and the other 2 groups were blank groups and model groups. The mice in the drug-administered groups and the model groups were bled 0.8mL from the retroorbital venous plexus to create an acute hemorrhagic blood deficiency model. The nano-egg-hide gelatin oral solution in each embodiment and comparative example was administered to the mice in the drug-administered groups by gavage, with a dosage of 0.5mL, once a day, and continued for 7 days; the blank group and the model group were given an equal amount of physiological saline, with a dosage of 0.5mL, once a day, and continued for 7 days. On the 1st, 3rd, and 7th days, the tails of the mice were respectively bled and the physical signs of each group of mice were recorded. The contents of red blood cells (RBC), hemoglobin (HGB) and platelet count (PLT) in the blood were determined using a blood cell analyzer, and the average value was taken for each group. The results of the determination are shown in Table 1.

[0072] Table 1 The contents of RBC, HGB and PLT in the blood of mice in each group

[0073]

[0074] As shown in Table 1, since acute massive bleeding generally leads to blood deficiency symptoms, in the blood deficiency mouse model, RBC, HGB and PLT in the mouse blood will decrease, so the RBC, HGB and PLT in the blood of the model group, the mice in Examples 1 to 3 and Comparative Example 1 will decrease after bleeding. However, after taking the oral liquid of Examples 1 to 3, the RBC, HGB and PLT in the blood of the mouse groups in Examples 1 to 3 and Comparative Example 1 gradually increased, and after 7 days of administration, the RBC, HGB and PLT in the blood basically returned to the level before bleeding, which shows that the nano donkey-hide gelatin oral liquid prepared in Examples 1 to 3 has a blood-tonifying effect on mice. It can be seen from the comparison of Examples 1 to 3 and Comparative Example 1 that the nano donkey-hide gelatin oral liquid prepared in Examples 1 to 3 has a better blood-tonifying effect on mice, which shows that the nano donkey-hide gelatin oral liquid prepared in the present invention has an excellent blood-tonifying effect.

[0075] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A nano donkey-hide gelatin oral liquid, characterized in that: The invention comprises the following raw materials in parts by weight: 50-150 parts of donkey-hide gelatin, 10-100 parts of jujube, 10-100 parts of wolfberry, 10-50 parts of hawthorn, 10-50 parts of allulose, 1-10 parts of astaxanthin, and 1-10 parts of surfactant.

2. The nano donkey-hide gelatin oral liquid according to claim 1, characterized in that: The surfactant includes egg yolk lecithin and / or soybean lecithin.

3. The method for preparing the nano donkey-hide gelatin oral liquid according to claim 1 or 2, characterized in that: The following steps are involved: S1, crushing the donkey-hide gelatin, mixing the crushed donkey-hide gelatin with a surfactant solution, and then subjecting the mixture to ultrasonic treatment to obtain coated donkey-hide gelatin particles; S2, mixing jujube, wolfberry and hawthorn and then crushing them to obtain a mixture, mixing the mixture, the coated donkey-hide gelatin particles and water and then extracting to obtain an extract; S3, concentrating the extract to obtain a concentrated solution; S4. The concentrated solution, allulose and astaxanthin are mixed and then subjected to nano-scale homogenization to obtain nano-donkey-hide gelatin oral solution.

4. The preparation method according to claim 3, characterized in that: In the step S1, the particle size of the crushed donkey-hide gelatin is 100 to 500 μm.

5. The preparation method according to claim 4, characterized in that: In the step S1, the mass concentration of the surfactant solution is 0.1-1%.

6. The preparation method according to claim 3 or 5, characterized in that: In the step S1, the ultrasonic treatment time is 10 to 20 minutes.

7. The preparation method according to claim 6, characterized in that: In the step S2, the particle size of the mixture is 100-500 μm.

8. The preparation method according to claim 3 or 7, characterized in that: In step S2, the mass ratio of the total mass of the mixture and the coated donkey-hide gelatin particles to water is 1:3-6; the extraction temperature is 60-80° C., and the extraction time is 60-120 min.

9. The preparation method according to claim 8, characterized in that: In step S3, the volume ratio of the concentrated solution to the extracted solution is 1:3-5.

10. The preparation method according to claim 9, characterized in that: In the step S4, the pressure of the nano-scale homogenization treatment is 50-100 MPa, and the number of times of the nano-scale homogenization treatment is 2-3 times.

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