Seawater rotifer cultivation method

Abstract

The seawater rotifer cultivation method comprises the steps that at least one supporting layer and at least one sacrificial layer arranged on the supporting layer are constructed, and the sacrificial layer is configured to be modified through changes of external conditions; cultivating a thin film on the sacrificial layer and growing the thin film; changing external conditions to modify the sacrificial layer and obtain an independent film; and cleaning and drying the obtained single film, and grinding and sieving to obtain the copper nitride powder. According to the method, the independent thin film is obtained, grinding and sieving are carried out on the basis of the thin film, so that the copper nitride powder is obtained, and compared with an existing method, the method has the advantages that the heating temperature is small, pressurization is not needed, energy consumption is small, environment friendliness is better achieved, operation is easy and convenient, practicability is high, and the yield is high, and has very high application prospects.

Description

Technical Field

[0001] The invention relates to the field of aquaculture, and in particular to a method for cultivating marine rotifers. Background Art

[0002] Rotifers are a type of plankton with rich nutritional value. They have many advantages, such as small size, fast reproduction and low cultivation cost. They are widely distributed. Because they contain amino acids and unsaturated fatty acids required by most aquatic animals, they have become the most palatable bait for aquatic animals in their larval stage. Compared with artificial baits, rotifer baits as live baits can naturally induce aquatic animals to eat, increase their feeding rate, and have little pollution to the water environment, reducing the difficulty of breeding management.

[0003] As the output of aquaculture continues to increase, the demand for rotifers is also increasing. Existing rotifer cultivation technology has many problems in large-scale aquaculture, including complex operation, low cultivation efficiency, frequent diseases and difficult water quality management, which cannot meet the needs of modern aquaculture, thus limiting the development of aquaculture. Moreover, most of the existing breeding technologies are freshwater breeding methods, and in the breeding process of marine aquaculture, the use of freshwater rotifers will cause large-scale death of rotifers, thus affecting the breeding of aquatic products. Therefore, how to propose a marine rotifer breeding method has become a technical problem that needs to be solved urgently in the industry. Summary of the invention

[0004] Purpose of the invention: In order to overcome the deficiencies in the prior art, the present invention provides a method for cultivating marine rotifers.

[0005] Technical solution: A method for cultivating marine rotifers, comprising the following steps:

[0006] S10: Disinfect the culture pool for rotifers;

[0007] S20: adding filtered seawater to the cultivation pool;

[0008] S30: inoculating Chlorella in the cultivation pond, so that the Chlorella in the water of the cultivation pond is evenly distributed to 2-4 million cells / ml;

[0009] S40: Inoculating rotifers: Select healthy and highly active rotifer populations and add them into the culture pond, and adjust the initial density to 50-100 rotifers / ml;

[0010] S50: Fertilization: Dissolve urea and potassium dihydrogen phosphate in a small amount of seawater, prepare dilutions, and slowly and evenly add them into the culture pond. 3 The concentration is controlled at 2-3ppm, KH 2 PO 4 The concentration is controlled at 0.2-0.3ppm;

[0011] S60: Feeding management: Feed with yeast and frozen concentrated Chlorella separately, 4-8 times a day, 15-30 grams of yeast powder per 100 million rotifers per day, and 200-400 grams of frozen concentrated Chlorella per 100 million rotifers per day;

[0012] S70: Add photosynthetic bacteria and Bacillus to the pool every day, the concentration of photosynthetic bacteria is controlled at 3-5ppm, and the concentration of Bacillus is controlled at 2-3ppm;

[0013] S80: After 4-8 days of culture, the cultured rotifers are harvested.

[0014] Furthermore, the step S10 further includes the following steps:

[0015] S11: Soak all equipment and ventilation devices in the culture tank in 20-50 mg / L potassium permanganate solution for 2-4 hours;

[0016] S12: Clean all equipment and ventilation devices in the culture tank with clean water;

[0017] S13: Spray the culture tank with 10-20 mg / L bleach solution;

[0018] S14: Soak the culture tank in a 10-20 mg / L bleaching powder solution for 12-14 hours, or soak the culture tank in a 20-50 mg / L potassium permanganate solution for 2-4 hours.

[0019] Furthermore, the step S20 also includes a filtering step for seawater:

[0020] S21: Remove large particles of impurities from seawater through a coarse mesh filter with a pore size of 50-100 μm;

[0021] S22: further filtering using a fiber or sand filter with a pore size of 5-10 μm;

[0022] S23: Add 20ppm bleaching powder and let stand for 8 hours;

[0023] S24: adding sodium thiosulfate to neutralize the residual chlorine to ensure that there is no toxic residue in the water body, and then adding the seawater treated as above into the cultivation pool.

[0024] Furthermore, after inoculation, the water temperature was maintained at 25-30°C and the dissolved oxygen concentration was greater than or equal to 1.2 mg / L.

[0025] Furthermore, the water pH was maintained at 7.5-8.5, and the concentrations of ammonia nitrogen and nitrite were maintained below 0.5 mg / L.

[0026] Furthermore, in step S60, the number of times the yeast is fed is less than or equal to half of the number of times the frozen concentrated Chlorella is fed.

[0027] Furthermore, the cultivation pool comprises: a pool body, a bottom of the pool body is provided with a settling part which decreases in height from the periphery of the pool body toward the center, a sewage outlet is provided at the bottom end of the settling part, and the sewage outlet is connected to an external sewage discharge device;

[0028] A gas output device for outputting gas;

[0029] An inflatable stone, wherein the inflatable stone is connected to the gas output device via an inflatable hose;

[0030] A vortex generating device is arranged at the edges of the bottom of the cultivation pool, and is used to generate a vortex for the water flow in the cultivation pool. The vortex generating device is connected to the gas output device.

[0031] Furthermore, the vortex generating device comprises an output pipe arranged parallel to the side wall of the cultivation tank, and a nanotube is arranged at the end of the output pipe.

[0032] Furthermore, the distance between the bottom end of the inflatable stone and the bottom surface of the sedimentation part is greater than or equal to 30 cm.

[0033] Beneficial effects: The present invention realizes precise control of the rotifer cultivation process through the above method, improves the rotifer cultivation environment, and enables rotifers to be cultivated in seawater, thereby providing a basis for marine aquaculture, and by feeding Chlorella, the mixed cultivation of rotifers and rotifer bait is realized, and the propagation capacity is strong. In particular, by adding photosynthetic bacteria and Bacillus, the water quality of the water body is improved, and Bacillus can produce a variety of substances with antibacterial, antiviral, insecticidal and other biological activities. In addition, Bacillus can also produce some enzymes, such as chitinase, β-1,3-glucanase, etc. These enzymes can decompose chitin and β-1,3-glucan, the main components of fungal cell walls, thereby destroying the cell wall structure of the fungus, causing the death of fungal cells, and achieving the purpose of preventing and controlling fungal diseases of crops. Photosynthetic bacteria can further provide a nitrogen source for Chlorella. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0035] Embodiment 1:

[0036] A method for cultivating marine rotifers comprises the following steps:

[0037] S01: construct a cultivation pool, the cultivation pool comprising: a pool body, a gas output device, an inflatable stone and a vortex generating device. The bottom of the pool body is provided with a settling part which decreases in height from the periphery of the pool body toward the center, and the bottom end of the settling part is provided with a sewage outlet which is connected to an external sewage discharge device.

[0038] A gas output device is used to output gas. The inflatable stone is connected to the gas output device through an inflatable hose. A vortex generating device is arranged at the edges of the bottom of the cultivation pool to create a vortex in the water flow in the cultivation pool, and the vortex generating device is connected to the gas output device. The vortex generating device includes an output tube arranged parallel to the side wall of the cultivation pool, and a nanotube is arranged at the end of the output tube. Because the nanotube itself has a plurality of small holes, when the airflow formed by the gas output device passes through the nanotube, a part of it will pass through the side wall of the nanotube to form bubbles, thereby generating a dissolved oxygen effect in the water body and increasing the oxygen content in the cultivation pool.

[0039] A vortex generating device is used to generate a vortex, and under the guidance of the sedimentation part, waste such as leftover bait and feces is directed to the sewage outlet, thereby maintaining continuous water flow and avoiding dead corners in the water body, thereby improving the distribution of dissolved oxygen and the efficiency of waste removal, thereby improving the quality level of aquaculture.

[0040] The distance between the bottom end of the inflatable stone and the bottom surface of the settlement part is equal to 30 cm.

[0041] S10: Disinfecting the rotifer cultivation pool. Specifically, the steps include:

[0042] S11: Soak all equipment and ventilation devices in the culture tank in 20 mg / L potassium permanganate solution for 4 hours;

[0043] S12: Clean all equipment and ventilation devices in the culture tank with clean water;

[0044] S13: Spray the culture tank with 20mg / L bleach solution;

[0045] S14: Soak the culture pool in a 20 mg / L bleach solution for 12 hours. In this way, the culture pool and related ventilation equipment are disinfected, the sanitary environment of the culture process is improved, and the mortality rate of rotifers during the culture process is reduced.

[0046] S20: adding filtered seawater to the cultivation pool, specifically, comprising the following steps:

[0047] S21: Remove large particles of impurities from seawater through a coarse mesh filter with a pore size of 50 μm;

[0048] S22: further filtering using a fiber or sand filter with a pore size of 5 μm;

[0049] S23: Add 20ppm bleaching powder and let stand for 8 hours;

[0050] S24: adding sodium thiosulfate to neutralize the residual chlorine to ensure that there is no toxic residue in the water body, and then adding the seawater treated as above into the cultivation pool.

[0051] The seawater treated as above reduces other harmful impurities while ensuring the salinity environment of the seawater, thereby improving the survival rate of rotifer culture.

[0052] S30: inoculating Chlorella in the cultivation pond so that the Chlorella in the water of the cultivation pond is evenly distributed to 4 million cells / ml;

[0053] S40: inoculating rotifers: selecting healthy and highly active rotifer populations and adding them into the culture pond, and adjusting the initial density to 100 rotifers / ml;

[0054] S50: Fertilization: Dissolve urea and potassium dihydrogen phosphate in a small amount of seawater, prepare dilutions, and slowly and evenly add them into the culture pond. 3 The concentration is controlled at 2ppm, KH 2 PO 4 The concentration is controlled at 0.2ppm;

[0055] S60: Feeding management: Feed with yeast and frozen concentrated Chlorella separately, 4-8 times a day, 15-30 grams of yeast powder per 100 million rotifers per day, and 200-400 grams of frozen concentrated Chlorella per 100 million rotifers per day; specifically, the yeast is fed twice a day, and the frozen concentrated Chlorella is fed four times a day.

[0056] S70: photosynthetic bacteria and Bacillus were added to the pool every day, the concentration of photosynthetic bacteria was controlled at 3ppm, and the concentration of Bacillus was controlled at 3ppm;

[0057] S80: After 4-8 days of culture, the cultured rotifers are harvested.

[0058] Furthermore, the water pH was maintained at 7.5-8.5, and the concentrations of ammonia nitrogen and nitrite were maintained below 0.5 mg / L.

[0059] Using the above scheme, the final density of rotifers harvested after 6 days of culture was 650 / ml, and the reproduction rate was 6.5 times.

[0060] Embodiment 2:

[0061] In this embodiment, S01: constructing a cultivation pool, which is different from Embodiment 1 in that: the distance between the bottom end of the inflatable stone and the bottom surface of the sedimentation part is equal to 50 cm.

[0062] S10: Disinfecting the rotifer cultivation pool. Specifically, the steps include:

[0063] S11: Soak all equipment and ventilation devices in the culture tank in 35 mg / L potassium permanganate solution for 4 hours;

[0064] S12: Clean all equipment and ventilation devices in the culture tank with clean water;

[0065] S13: Spray the culture tank with 10mg / L bleach solution;

[0066] S14: Soak the culture pool in a 10 mg / L bleach solution for 14 hours. In this way, the culture pool and related ventilation equipment are disinfected, the sanitary environment of the culture process is improved, and the mortality rate of rotifers during the culture process is reduced.

[0067] S20: adding filtered seawater to the cultivation pool, specifically, comprising the following steps:

[0068] S21: Remove large particles of impurities from seawater through a coarse mesh filter with a pore size of 100 μm;

[0069] S22: further filtering using a fiber or sand filter with a pore size of 10 μm;

[0070] S23: Add 20ppm bleaching powder and let stand for 8 hours;

[0071] S24: adding sodium thiosulfate to neutralize the residual chlorine to ensure that there is no toxic residue in the water body, and then adding the seawater treated as above into the cultivation pool.

[0072] The seawater treated as above reduces other harmful impurities while ensuring the salinity environment of the seawater, thereby improving the survival rate of rotifer culture.

[0073] S30: inoculating Chlorella in the cultivation pond so that the Chlorella in the water of the cultivation pond is evenly distributed to 2 million cells / ml;

[0074] S40: Inoculating rotifers: Select healthy and highly active rotifer populations and add them to the culture pond, and adjust the initial density to 500 rotifers / ml; and maintain the water temperature at 25-30°C and the dissolved oxygen concentration at or above 1.2 mg / L after inoculation.

[0075] S50: Fertilization: Dissolve urea and potassium dihydrogen phosphate in a small amount of seawater, prepare dilutions, and slowly and evenly add them into the culture pond. 3 The concentration is controlled at 3ppm, KH 2 PO 4 The concentration is controlled at 0.3ppm;

[0076] S60: Feeding management: Feed with yeast and frozen concentrated Chlorella separately, feed 8 times a day, 30 grams of yeast powder per 100 million rotifers per day, and 400 grams of frozen concentrated Chlorella per 100 million rotifers per day; specifically, the yeast feeding frequency is 3 times a day, and the frozen concentrated Chlorella feeding frequency is 5 times a day.

[0077] S70: Add photosynthetic bacteria and Bacillus to the pool every day, the concentration of photosynthetic bacteria is controlled at 4ppm, and the concentration of Bacillus is controlled at 2ppm;

[0078] S80: After 6 days of culture, the final density of rotifers harvested was 2500 / ml, and the reproduction rate was 5 times.

[0079] Example 3

[0080] S01: Constructing a cultivation pool. The difference between the construction of the cultivation pool and the embodiment 1 is that the distance between the bottom end of the inflatable stone and the bottom surface of the sedimentation part is equal to 40 cm.

[0081] S10: Disinfecting the rotifer cultivation pool. Specifically, the steps include:

[0082] S11: Soak all equipment and ventilation devices in the culture tank in 50 mg / L potassium permanganate solution for 3 hours;

[0083] S12: Clean all equipment and ventilation devices in the culture tank with clean water;

[0084] S13: The culture pool is sprayed with 15 mg / L bleach solution;

[0085] S14: Soak the culture pool in a 15 mg / L bleach solution for 13 hours. In this way, the culture pool and related ventilation equipment are disinfected, the sanitary environment of the culture process is improved, and the mortality rate of rotifers during the culture process is reduced.

[0086] S20: adding filtered seawater to the cultivation pool, specifically, comprising the following steps:

[0087] S21: Remove large particles of impurities from seawater through a coarse mesh filter with a pore size of 75 μm;

[0088] S22: further filtering using a fiber or sand filter with a pore size of 8 μm;

[0089] S23: Add 20ppm bleaching powder and let stand for 8 hours;

[0090] S24: adding sodium thiosulfate to neutralize the residual chlorine to ensure that there is no toxic residue in the water body, and then adding the seawater treated as above into the cultivation pool.

[0091] The seawater treated as above reduces other harmful impurities while ensuring the salinity environment of the seawater, thereby improving the survival rate of rotifer culture.

[0092] S30: inoculating Chlorella in the cultivation pond so that the Chlorella in the water of the cultivation pond is evenly distributed to 3 million cells / ml;

[0093] S40: Inoculating rotifers: Select healthy and highly active rotifer populations and add them into the culture pond, and adjust the initial density to 75 rotifers / ml;

[0094] S50: Fertilization: Dissolve urea and potassium dihydrogen phosphate in a small amount of seawater, prepare dilutions, and slowly and evenly add them into the culture pond. 3 The concentration is controlled at 2.5ppm, KH 2 PO 4 The concentration is controlled at 0.25ppm;

[0095] S60: Feeding management: Feed with yeast and frozen concentrated Chlorella respectively, 4 times a day, 15-30 grams of yeast powder per 100 million rotifers per day, and 200-400 grams of frozen concentrated Chlorella per 100 million rotifers per day; specifically, the yeast is fed once a day, and the frozen concentrated Chlorella is fed 3 times a day.

[0096] S70: photosynthetic bacteria and Bacillus were added to the pool every day, the concentration of photosynthetic bacteria was controlled at 5ppm, and the concentration of Bacillus was controlled at 2.5ppm;

[0097] S80: After 4-8 days of culture, the cultured rotifers are harvested.

[0098] Furthermore, the pH of the water was maintained at 8, and the concentrations of ammonia nitrogen and nitrite were maintained below 0.5 mg / L. After 6 days of cultivation, the final density of the rotifers harvested was 400 per milliliter, and the reproduction rate was 5.3 times.

[0099] Control group:

[0100] No air stone hanging, soak all equipment and ventilation devices with 20mg / L potassium permanganate solution for 4h, then rinse with clean water. Use 20mg / l bleach solution to spray and disinfect the cultivation pool. Add seawater that has been coarsely filtered through 100um and finely filtered through 20um, and then disinfected with 20ppm bleach and neutralized with sodium thiosulfate. Inoculate Chlorella to a density of 4 million cells / ml, and inoculate rotifers to adjust its initial density to 100 cells / ml. Use urea and potassium dihydrogen phosphate as fertilizers, control the NaNO3 concentration at 2ppm, and the KH2PO4 concentration at 0.2ppm. Feed yeast twice a day and frozen concentrated Chlorella 4 times. Feed 15 grams of yeast powder and 200 grams of frozen concentrated Chlorella for every 100 million rotifers. Add photosynthetic bacteria and Bacillus to the pool every day, and control the concentration of photosynthetic bacteria at 3ppm and the concentration of Bacillus at 3ppm. The water pH was maintained at 7.5-8.5, and the concentrations of ammonia nitrogen and nitrite were kept below 0.5 mg / L. After 6 days of culture, the final density of rotifers harvested was 300 / ml, and the reproduction rate was 3 times.

[0101] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for cultivating marine rotifers, characterized in that: The following steps are involved: S10: Disinfect the culture pool for rotifers; S20: adding filtered seawater to the cultivation pool; S30: inoculating Chlorella in the cultivation pond, so that the Chlorella in the water of the cultivation pond is evenly distributed to 2-4 million cells / ml; S40: Inoculating rotifers: Select healthy and highly active rotifer populations and add them into the culture pond, and adjust the initial density to 50-100 rotifers / ml; S50: Fertilization: Dissolve urea and potassium dihydrogen phosphate in a small amount of seawater, prepare diluents, and slowly and evenly add them into the cultivation pond, wherein the concentration of NaNO3 is controlled at 2-3ppm and the concentration of KH2PO4 is controlled at 0.2-0.3ppm; S60: Feeding management: Feed with yeast and frozen concentrated pellets separately, 4-8 times a day in total, 15-30 grams of yeast powder per 100 million rotifers per day, and 200-400 grams of frozen concentrated chlorella per 100 million rotifers per day; S70: Add photosynthetic bacteria and Bacillus to the pool every day, the concentration of photosynthetic bacteria is controlled at 3-5ppm, and the concentration of Bacillus is controlled at 2-3ppm; S80: After 4-8 days of culture, the cultured rotifers are harvested.

2. The method for cultivating marine rotifers according to claim 1, characterized in that: The step S10 further includes the following steps: S11: Soak all equipment and ventilation devices in the culture tank in 20-50 mg / L potassium permanganate solution for 2-4 hours; S12: Clean all equipment and ventilation devices in the culture tank with clean water; S13: Spray the culture tank with 10-20 mg / L bleach solution; S14: Soak the culture tank in a 10-20 mg / L bleaching powder solution for 12-14 hours, or soak the culture tank in a 20-50 mg / L potassium permanganate solution for 2-4 hours.

3. The method for cultivating marine rotifers according to claim 1, characterized in that: The step S20 also includes a filtering step for seawater: S21: Remove large particles of impurities from seawater through a coarse mesh filter with a pore size of 50-100 μm; S22: further filtering using a fiber or sand filter with a pore size of 5-10 μm; S23: Add 20ppm bleaching powder and let stand for 8 hours; S24: adding sodium thiosulfate to neutralize the residual chlorine to ensure that there is no toxic residue in the water body, and then adding the seawater treated as above into the cultivation pool.

4. The method for cultivating marine rotifers according to claim 1, characterized in that: After inoculation, maintain a water temperature of 25-30°C and a dissolved oxygen concentration greater than or equal to 1.2 mg / L.

5. The method for cultivating marine rotifers according to claim 1, characterized in that: Maintain the water pH at 7.5-8.5, and keep the ammonia nitrogen and nitrite concentrations below 0.5 mg / L.

6. The method for cultivating marine rotifers according to claim 1, characterized in that: In step S60, the number of times the yeast is fed is less than or equal to half of the number of times the frozen concentrated Chlorella is fed.

7. The method for cultivating marine rotifers according to any one of claims 1 to 6, characterized in that: The cultivation pool comprises: a pool body, a bottom of the pool body is provided with a settling part which decreases in height from the periphery of the pool body toward the center, a sewage outlet is provided at the bottom end of the settling part, and the sewage outlet is connected to an external sewage discharge device; A gas output device for outputting gas; An inflatable stone, wherein the inflatable stone is connected to the gas output device via an inflatable hose; A vortex generating device is arranged at the edges of the bottom of the cultivation pool, and is used to generate a vortex for the water flow in the cultivation pool. The vortex generating device is connected to the gas output device.

8. The method for cultivating marine rotifers according to claim 6, characterized in that: The vortex generating device comprises an output pipe which is arranged parallel to the side wall of the cultivation tank, and a nanotube is arranged at the end of the output pipe.

9. The method for cultivating marine rotifers according to claim 6, characterized in that: The distance between the bottom end of the inflatable stone and the bottom surface of the settlement part is greater than or equal to 30 cm.