Radio stimulation surgical suture for promoting peripheral nerve healing and preparation method and application thereof

Abstract

The invention relates to the technical field of peripheral nerve injury suture, and particularly discloses a radio stimulation surgical suture for promoting peripheral nerve healing and a preparation method and application thereof, the radio stimulation surgical suture comprises a Mo core layer and a PLGA coating; the preparation method of the radio stimulation operation suture comprises the following steps: preparing a core layer by using metal Mo, spraying out a PLGA spinning solution through a spinning nozzle, curing in a coagulating bath to form a film, and forming a uniformly coated PLGA coating on the Mo core layer through a wet spinning technology to obtain the radio stimulation operation suture for promoting peripheral nerve healing, according to the prepared radio stimulation surgical suture, energy is obtained from an external electromagnetic field in an electromagnetic induction and capacitance coupling mode, and a stable electric field is formed on the suture and the local part of a wound, so that the electric field acts on damaged tissue. The suture can promote neuronal axon growth, Schwann cell migration and the like, and the nerve tissue regeneration efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of peripheral nerve injury suturing, and more specifically, to a radio-stimulation surgical suture for promoting peripheral nerve healing, and a preparation method and application thereof. Background Art

[0002] Peripheral nerve injury (PNI) is one of the common clinical problems worldwide. Every year, more than 1 million people suffer peripheral nerve damage. This injury can be caused by a variety of factors, including traumatic accidents, tumor damage, surgical injuries, etc. Although a large number of studies have proposed different treatment options, clinical treatment of large lesions and long-segment peripheral nerve injuries still faces huge challenges. How to effectively repair these injuries and restore their functions remains a major problem that needs to be solved in the global medical field.

[0003] In the human body, bioelectricity is one of the basic components of organisms. Cell membrane potential plays a vital role in the regulation of cell cycle, migration, proliferation and differentiation. Action potential determines the excitability of cells, especially in nerve cells. Specifically, bioelectricity regulates a variety of biological processes, such as information exchange between synapses and neurons and the function of nerve tissue cells. With the in-depth study of natural bioelectricity and biopiezoelectric phenomena, electroactive biomaterials, as an interdisciplinary subject of molecular chemistry and physics, engineering materials, biology and medicine, have gradually become a research hotspot. These materials include biocompatible conductive materials and piezoelectric materials, which have made significant progress in the design and preparation of biomaterial scaffolds. Studies have shown that low-frequency pulse electrical stimulation can significantly promote the regeneration process of peripheral nerve injury. Providing electrical stimulation through these electroactive biomaterials to promote nerve repair has great application potential.

[0004] However, due to the diversity of PNI sites and the long functional reconstruction cycle, existing electrical stimulation devices and electrodes face many limitations in clinical applications and may cause serious complications, including inflammation, immune rejection, and pain. In addition, most existing electrical stimulation schemes rely on percutaneous electrode wires to transmit electrical stimulation signals to the injured area. This method not only has a high risk of infection, but also requires a second surgery to remove the implanted electrodes after tissue repair is completed, which greatly limits its clinical application.

[0005] In response to the above problems, researchers have proposed many innovative solutions, especially in the development of radio stimulation systems. Currently, exploring radio stimulation systems that do not require external power sources and percutaneous electrode wires has become a current research hotspot. However, current wearable or implantable electrical stimulation systems still face various technical limitations. For example, most systems can only provide simple electrical pulse stimulation, and the stimulation intensity and frequency are fixed, lacking the ability to change synchronously with the physiological state, thus affecting the repair effect. In addition, most of these electrical stimulation systems rely on specific power supply devices to provide energy. For example, a type of absorbable patch electrode reported by Lee et al. requires an external power supply. To improve this defect, Kim et al. adopted a non-invasive method, that is, they developed a patch electrode that uses ultrasonic transmission of vibration to generate electrical stimulation through the principle of triboelectricity, but still needs to provide energy for the device at the wound through an ultrasonic probe during treatment, so this type of problem has not been fundamentally solved. These problems limit their application in nerve function reconstruction.

[0006] The patent with the publication number CN110170068A discloses a multi-functional medical suture and its preparation method, including a suture body, multiple adhesion coatings, and drug-loaded nanoparticles. This medical suture can enhance the healing effect of tissues such as tendon tissue, muscle tissue, and epidermal tissue through sustained-release nanoparticles loaded with different drugs, and can also effectively inhibit the early inflammatory reaction of the injury and relieve the pain in the later stage. However, for suturing nerve tissue, this suture cannot provide a stable drug release concentration for tissue healing, nor can it provide the electrical cues necessary for the regeneration process of nerve tissue to guide the nerve regeneration process, so it is difficult to increase the regeneration speed after peripheral nerve injury. Summary of the Invention

[0007] To solve the shortcomings that existing medical sutures for suturing nerve tissue can neither provide a stable drug release concentration for tissue healing nor provide the electrical cues necessary for the regeneration process of nerve tissue to stimulate, and it is difficult to increase the regeneration speed after peripheral nerve injury, the present invention provides a radio stimulation surgical suture for promoting peripheral nerve healing, its preparation method, and application. This suture includes a Mo core layer and a PLGA coating, which can not only provide electrical stimulation to promote tissue healing but also gradually degrade in the body without the need for additional removal. The core working principle of the suture of the present invention is that, due to the difference in the dielectric constants of the suture and human tissue, a potential difference is formed between the suture and human tissue under the action of an external electromagnetic field (such as the electric field released by electronic devices such as mobile phones and computers), thereby generating controllable electrical stimulation to promote nerve tissue healing.

[0008] The present invention provides a radio stimulation surgical suture for promoting peripheral nerve healing, adopting the following technical solutions: A radiofrequency-stimulating surgical suture for promoting peripheral nerve healing, comprising a Mo core layer and a PLGA coating.

[0009] Preferably, the radiofrequency stimulation principle is the human body coupling principle of an external electromagnetic field.

[0010] Preferably, the Mo core layer is used to provide electrical stimulation and improve the mechanical strength and conductivity of the suture.

[0011] Preferably, the PLGA coating is used to enhance biocompatibility, reduce the inflammatory reaction caused by metal ion release, and regulate the degradation rate of the suture.

[0012] A preparation method of a radiofrequency-stimulating surgical suture for promoting peripheral nerve healing, comprising the following steps: Using a metal Mo filament as the Mo core layer, spraying the PLGA spinning solution through a spinneret, and curing and forming a film in a coagulation bath, and forming a uniformly coated PLGA coating on the Mo core layer through wet spinning technology to obtain a radiofrequency-stimulating surgical suture for promoting peripheral nerve healing.

[0013] Preferably, the PLGA spinning solution is made by mixing PLGA and hexafluoroisopropanol.

[0014] Preferably, the mass fraction of the PLGA spinning solution is 4-20%.

[0015] Preferably, the mass fraction of the PLGA spinning solution is 12%.

[0016] Preferably, the diameter of the radiofrequency-stimulating surgical suture for promoting peripheral nerve healing is 50-200 μm, suitable for microsurgical suture procedures.

[0017] Preferably, the diameter of the Mo core layer is 30-70 μm.

[0018] Preferably, the diameter of the Mo core layer is 50 μm.

[0019] Preferably, the process parameters of the wet spinning are as follows: a single-hole spinneret, the pore diameter is 140-160 μm, and the drawing speed is 5-8 r / min.

[0020] Preferably, the process parameters of the wet spinning are as follows: a single-hole spinneret, the pore diameter is 150 μm, and the drawing speed is 6 r / min.

[0021] Preferably, the coagulation bath is 90-98% ethanol, and the draw ratio is 1-1.2.

[0022] Preferably, the coagulation bath is 95% ethanol, and the draw ratio is 1.

[0023] Application of a radio - stimulated surgical suture for promoting peripheral nerve healing in the repair of peripheral nerve injuries.

[0024] Preferably, the application method is as follows: Use the radio - stimulated surgical suture for promoting peripheral nerve healing to suture the peripheral nerve injury site, and then provide electrical stimulation under the action of an external electromagnetic field to promote the recovery and regeneration of nerve tissue.

[0025] Preferably, the peripheral nerve injuries include facial nerve, brachial plexus nerve, sciatic nerve, etc.

[0026] Preferably, in the process of realizing radio - stimulation of the radio - stimulated surgical suture for promoting peripheral nerve healing in vivo, an electrical stimulation of 2 - 8V can be generated under the action of an external electromagnetic field to promote axon growth, myelin regeneration, and neurovascular regeneration.

[0027] In summary, the present invention has the following beneficial effects: The radio - stimulated surgical suture for promoting peripheral nerve healing prepared by the present invention obtains energy from an external electromagnetic field through electromagnetic induction and capacitive coupling, and forms a stable electric field at the suture and the wound local area, thereby acting on the damaged tissue. The suture of the present invention can promote neuron axon growth, Schwann cell migration, etc., and improve the efficiency of nerve tissue regeneration. Compared with traditional implantable electrical stimulation devices, the human - coupled radio - stimulated surgical suture of the present invention does not require an additional implanted power source, and can provide energy only through an external electromagnetic field (such as the electric field released by electronic devices such as mobile phones and computers), simplifying the clinical application process and having broad application prospects in peripheral nerve repair.

[0028] The present invention utilizes the excellent electrical conductivity, good mechanical properties, and reliable biosecurity of Mo to improve the transmission efficiency of electrical stimulation and enhance the electrical regulation effect on nerves or other tissues; Mo has high strength and toughness. Compared with other degradable metals (such as magnesium), the mechanical properties of Mo are more stable, not easily broken or damaged, which helps to maintain the structural integrity and long - term stability of the radio - stimulated surgical suture; a low dose of Mo element has good biocompatibility.

[0029] The present invention uses a PLGA coating to improve biocompatibility, reduce the inflammatory reaction that may be brought by metal ions, regulate the degradation rate of Mo, reduce the potential toxicity brought by metal release, enhance mechanical strength, reduce the risk of suture breakage, improve the stability of electrical stimulation, and optimize the function of the radio - stimulated surgical suture. The surgical suture of the present invention can naturally degrade after completing the repair task, avoiding secondary surgery for removal. Description of the Drawings

[0030] Figure 1Schematic diagram of the principle of the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing to generate electrical stimulation on human tissues under the action of an external electromagnetic field.

[0031] Figure 2 Circuit diagram of the principle of the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing to generate electrical stimulation on human tissues under the action of an external electromagnetic field.

[0032] Figure 3 Schematic diagram of the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing.

[0033] Figure 4 Surgical procedure diagram of the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing used for peripheral nerve suture.

[0034] Figure 5 Diagram of the results of tissue sampling, HE staining, and toluidine blue staining of the rat facial nerves sutured with the surgical sutures prepared in Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 of the present invention.

[0035] Figure 6 Immunofluorescence diagram of the structural protein expression of the rat facial nerves sutured with the surgical sutures prepared in Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 of the present invention.

[0036] Figure 7 Diagram of the effect of the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing on the survival rate of in - vitro cultured cells.

[0037] Figure 8 Comparison diagram of HE staining of the main organs of rats and healthy rats after the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing was applied in rats.

[0038] Figure 9 Diagram of the variation range of the voltage magnitude generated by the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing in vitro with the distance from the electromagnetic field emission source.

[0039] Figure 10 Diagram of the change trend of the voltage magnitude generated by the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing with the degradation time during in - vivo degradation.

[0040] Figure 11 Comparison diagram of the maximum load - bearing capacity of the radio - stimulation surgical suture prepared in Example 1 of the present invention for promoting peripheral nerve healing and commercially available surgical sutures under static stretching conditions.

[0041] Figure 12 Stiffness comparison diagram of the radiofrequency stimulation surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention and a commercially available surgical suture. Detailed implementation mode

[0042] Refer to the attached Figures 1-12 , and the present invention will be further described in detail below in conjunction with embodiments.

[0043] The sources of the raw materials used in the embodiments of the present invention are as follows: The metal Mo is molybdenum wire, purchased from Wuxi Shengshida New Material Co., Ltd.

[0044] Poly(lactic-co-glycolic acid) (PLGA), purchased from Nature Works, USA.

[0045] Hexafluoroisopropanol, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0046] Ethanol, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0047] Commercially available surgical suture (suture with a needle; model: O3 / 8, 9-0), purchased from Ningbo Medical Sewing Needle Co., Ltd.

[0048] Commercially available nylon suture (9-0), purchased from Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.

[0049] Commercially available absorbable suture sample (9-0), purchased from Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.

[0050] Example 1 A radiofrequency stimulation surgical suture for promoting peripheral nerve healing, comprising a Mo core layer and a PLGA coating; A preparation method of a radiofrequency stimulation surgical suture for promoting peripheral nerve healing, comprising the following steps: At room temperature, PLGA and hexafluoroisopropanol are mixed to prepare a PLGA spinning solution with a mass fraction of 12%; Using molybdenum wire as the Mo core layer, the PLGA spinning solution is ejected through a spinneret and solidified into a film in a coagulation bath, and a uniformly coated PLGA coating is formed on the Mo core layer by wet spinning technology to obtain a radiofrequency stimulation surgical suture with a diameter of 120 μm for promoting peripheral nerve healing; Among them, the process parameters of wet spinning are as follows: draw wet spinning, use a glass cavity with a replaceable spinneret as a container, pour the biodegradable polymer PLGA spinning solution into the glass cavity and fix it on the spinning table, the Mo core layer enters from the upper end and is drawn out from the metal spinning needle head at the bottom of the cavity, a single-hole spinneret, pore diameter 150 μm, room temperature, drawing speed 6 r / min.

[0051] The coagulation bath is 95% ethanol for coagulation. The drawn molybdenum wire is treated in the coagulation bath and then washed in a room temperature water bath. The draw ratio is 1.

[0052] A method for applying a radio-stimulated surgical suture for promoting peripheral nerve healing is as follows: The main trunk of the facial nerve of a rat is transected to make a rat facial nerve transection model. A radio-stimulated surgical suture is used to suture the transected facial nerve of the rat. The rat is exposed to an adjustable electromagnetic field for 2 h every day, so that the suture performs electrostimulation treatment on the transected nerve of the rat to promote nerve tissue growth.

[0053] Example 2 A radio-stimulated surgical suture for promoting peripheral nerve healing includes a Mo core layer and a PLGA coating; A method for preparing a radio-stimulated surgical suture for promoting peripheral nerve healing includes the following steps: At room temperature, PLGA and hexafluoroisopropanol are mixed to make a PLGA spinning solution with a mass fraction of 4%; Using molybdenum wire as the Mo core layer, the PLGA spinning solution is ejected through a spinneret and solidified into a film in a coagulation bath. A uniformly coated PLGA coating is formed on the Mo core layer by wet spinning technology to obtain a radio-stimulated surgical suture with a diameter of 100 μm for promoting peripheral nerve healing; Among them, the process parameters of wet spinning are as follows: draw wet spinning, use a glass cavity with a replaceable spinneret as a container, pour the biodegradable polymer PLGA spinning solution into the glass cavity and fix it on the spinning table. The Mo core layer enters from the upper end and is drawn out from the metal spinning needle at the bottom of the cavity. Single-hole spinneret, aperture 150 μm, room temperature, draw speed 8 r / min.

[0054] The coagulation bath is 90% ethanol for coagulation. The drawn molybdenum wire is treated in the coagulation bath and then washed in a room temperature water bath. The draw ratio is 1.2.

[0055] Example 3 A radio-stimulated surgical suture for promoting peripheral nerve healing includes a Mo core layer and a PLGA coating; A method for preparing a radio-stimulated surgical suture for promoting peripheral nerve healing includes the following steps: At room temperature, PLGA and hexafluoroisopropanol are mixed to make a PLGA spinning solution with a mass fraction of 20%; Using molybdenum wire as the Mo core layer, the PLGA spinning solution is ejected through a spinneret and solidified into a film in a coagulation bath. A uniformly coated PLGA coating is formed on the Mo core layer through wet spinning technology to obtain a radio-stimulated surgical suture with a diameter of 150 μm for promoting peripheral nerve healing; Among them, the process parameters of wet spinning are as follows: Tension wet spinning, using a glass cavity with a replaceable spinneret as the container, pouring the biodegradable polymer PLGA spinning solution into the glass cavity and fixing it on the spinning table. The Mo core layer enters from the upper end and is drawn out from the metal spinning needle at the bottom of the cavity. Single-hole nozzle, aperture 150 μm, room temperature, drawing speed 5 r / min.

[0056] The coagulation bath is 98% ethanol coagulation. The drawn molybdenum wire is treated in the coagulation bath and then washed in a room temperature water bath. The draw ratio is 1.1.

[0057] Comparative Example 1 An application method of a radio-stimulated surgical suture for promoting peripheral nerve healing is as follows: Use the radio-stimulated surgical suture for promoting peripheral nerve healing in Example 1 to suture the transected facial nerve of rats. The rats are raised under normal conditions without being exposed to electromagnetic fields.

[0058] Comparative Example 2 An application method of a commercially available surgical suture is as follows: Use a commercially available surgical suture (suture with a needle; model: O3 / 8, 9-0; purchased from Ningbo Medical Suture Co., Ltd.) to suture the transected facial nerve of rats. The rats are raised under normal conditions without being exposed to electromagnetic fields.

[0059] Comparative Example 3 An application method of a commercially available surgical suture is as follows: Use a commercially available surgical suture (suture with a needle; model: O3 / 8, 9-0; purchased from Ningbo Medical Suture Co., Ltd.) to suture the transected facial nerve of rats. The rats are exposed to an adjustable electromagnetic field for 2 h every day for electromagnetic field exposure treatment.

[0060] Experiment 1. Healing effect of radio-stimulated surgical suture on rat cross-sectional nerve tissue One month later, observe the healing of the cross-sectional nerve tissue of rats sutured with the sutures in Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3, and the expression of main structure and function proteins. The specific results are shown in Table 1 below and Appendix Figures 5-6 。

[0061] Table 1:

[0062] From Table 1 and Appendix Figures 5-6 The displayed data show that: after regular exposure to electromagnetic fields for electrical stimulation treatment, the transected facial nerve of rats was sutured using the radio-frequency stimulation surgical sutures for promoting peripheral nerve healing in Example 1 of the present invention. The nerve tissue recovery speed was significantly accelerated, the regenerated nerve tissue maturity was higher, and the facial nerve injury effect of the treated rats was better. In Comparative Example 1, the transected facial nerve of rats was sutured using the radio-frequency stimulation surgical sutures for promoting peripheral nerve healing in Example 1. However, since the rats were not exposed to electromagnetic fields, the effect of electrical stimulation treatment on the wound was not produced, the nerve maturity was poor, and the final effect was poor. In Comparative Example 2, commercially available surgical sutures (suture needles with thread; models: O3 / 8, 9-0; purchased from Ningbo Medical Suture Needle Co., Ltd.) were used to suture the transected facial nerve of rats. In Comparative Example 3, commercially available surgical sutures (suture needles with thread; models: O3 / 8, 9-0; purchased from Ningbo Medical Suture Needle Co., Ltd.) were used to suture the transected facial nerve of rats and an electric field was applied. The experimental results showed that the tissue recovery effect and neuron maturity of Comparative Examples 2 and 3 were not as good as those of Example 1, indicating that Comparative Examples 2 and 3 could not generate electrical stimulation treatment at the wound, so the nerve tissue regeneration was slow and the therapeutic effect was not good.

[0063] Attached Figure 5 HE staining of nerve tissue sections showed that compared with the different treatment groups, Example 1 had less inflammation in the nerve tissue, a higher proportion of nerve tissue area, and more orderly arrangement of nerve fibers, while the other treatment groups had obvious inflammation at the nerve transection site, a smaller proportion of nerve tissue area, and disordered arrangement of nerve fibers.

[0064] Experiment 2: Effect of radio frequency stimulation of surgical sutures on the survival rate of cells cultured in vitro PC12 cells were cultured in vitro, and the radio-frequency stimulation surgical suture for promoting peripheral nerve healing in Example 1 was added to the cell culture medium, and the mass ratio of the radio-frequency stimulation surgical suture to the culture medium was controlled to be 1:9. After culturing for 24 hours, the culture medium was discarded, and the live cells and dead cells were stained using a cell viability kit, and the live / dead cell ratio was observed using a fluorescence microscope. The specific results are shown in the attached Figure 7 .

[0065] By the attached Figure 7 It can be seen that after co-culturing cells with radio-stimulated surgical sutures, the number of dead cells (red) is very low compared to the number of living cells (green).

[0066] Experiment 3: Comparison of HE staining images of the main organs of rats treated with radio-stimulated surgical sutures and healthy rats without any treatment Two months after using radio-stimulated surgical sutures to suture the transected facial nerve of rats, the rats were sacrificed and their hearts, livers, spleens, lungs and kidneys were removed. After fixation with paraformaldehyde, paraffin sections were prepared, stained with HE, and compared with the HE-stained sections of the main organs of healthy rats without any treatment, in order to observe whether the metabolites of radio-stimulated surgical sutures in vivo would be toxic to the main organs of rats. The specific results are shown in the appendix Figure 8 .

[0067] As shown in the appendix Figure 8 It can be seen that there were no inflammations or other lesions in the main organs of rats treated with radio-stimulated surgery for transected facial nerves, and the organ structures were basically the same as those of healthy rats

[0068] Experiment 4. Influence of the voltage generated by radio-stimulated surgical sutures in vitro on the distance from the electromagnetic field emission source Prepare several samples of radio-stimulated surgical sutures. Use an electromagnetic field generator with a stable output frequency as the magnetic field source. Suture the suture samples into the rats' bodies, leaving a section of the suture for connecting to the oscilloscope. Place the rats at distances of 10 cm, 20 cm, 30 cm, 40 cm, and 50 cm from the emission source respectively, and record the peak voltage induced on the suture at each distance through the oscilloscope. Each group of experiments was repeated 3 times and the average value was taken. The specific results are shown in the appendix Figure 9 .

[0069] As shown in the appendix Figure 9 It can be seen that the voltage induced by the radio-stimulated suture gradually decreases as the distance from the electromagnetic field source increases. The appendix Figure 9 shows that when the distance from the emission source is 10 cm, the maximum voltage (about 7.8 V) can be induced on the suture; when the distance reaches 50 cm, the induced voltage drops to the lowest (about 1.6 V). This result indicates that the radio-stimulated suture has a relatively significant induction response ability within 50 cm and is suitable for clinical tissue suture environments at a certain depth

[0070] Experiment 5. Influence of the voltage generated by radio-stimulated surgical sutures during degradation in vivo on the degradation time Implant radio-stimulated sutures into the subcutaneous tissue of rats. At different time points within 70 days, anesthetize the animals and expose the remaining suture sites. Use an external electromagnetic field to stimulate the sutures, connect electrodes at both ends of the sutures, and record the voltage generated by the sutures through the oscilloscope. Record the peak value of the induced voltage and compare it with the initial value. The specific results are shown in the appendix Figure 10 .

[0071] As shown in the appendix Figure 10 It can be seen that as the implantation time prolongs, the induced voltage of the suture does not change significantly compared with the initial value, indicating that its metabolic rate is slower than the nerve healing rate, which can ensure a stable therapeutic effect during nerve recovery

[0072] Experiment 6: Comparison of the mechanical properties between the radio-stimulating surgical suture and commercially available surgical sutures Prepare several samples of radio-stimulating surgical sutures, commercially available nylon sutures (Model: O3 / 8, 9-0, Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.), and commercially available absorbable sutures (Model: O3 / 8, 9-0, Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.). Conduct tensile tests using an electronic tensile tester. Record indicators such as the maximum tensile strength, elongation at break, and elastic modulus of each suture. The specific results are shown in the appendix Figures 11-12 .

[0073] As can be seen from the appendix Figures 11-12 It can be known that the appendix Figure 11 shows that the maximum tensile strength of the radio-stimulating surgical suture is significantly higher than that of the two commercially available sutures, indicating that it has good mechanical support during the suturing process. The appendix Figure 12 further shows that its elastic modulus and elongation at break are comparable to those of the two commercially available sutures, and it has good toughness and tensile adaptability. The overall results show that although the suture is embedded with conductive components, it will not affect its basic mechanical properties and is suitable for clinical suturing use

[0074] This specific embodiment is only an explanation of the present invention and is not a limitation thereof. Those skilled in the art can make modifications to this embodiment without creative contributions according to their needs after reading this specification, but as long as they are within the scope of the claims of the present invention, they are protected by the patent law

Claims

1. A radio-stimulation surgical suture for promoting peripheral nerve healing, characterized in that: It includes a Mo core layer and a PLGA coating layer.

2. A method for preparing a radio-stimulation surgical suture for promoting peripheral nerve healing according to claim 1, characterized in that: The following steps are involved: Metal Mo is used as the Mo core layer, the PLGA spinning solution is ejected through a spinneret and solidified into a film in a coagulation bath, and a uniformly coated PLGA coating is formed on the Mo core layer by wet spinning technology to obtain a radio-frequency stimulation surgical suture for promoting peripheral nerve healing.

3. The method for preparing a radio-frequency stimulation surgical suture for promoting peripheral nerve healing according to claim 2, characterized in that: The PLGA spinning solution is prepared by mixing PLGA and hexafluoroisopropanol; the mass fraction of the PLGA spinning solution is 4-20%.

4. The method for preparing a radio-stimulation surgical suture for promoting peripheral nerve healing according to claim 2, characterized in that: The diameter of the radio frequency stimulation surgical suture for promoting peripheral nerve healing is 50-200 μm.

5. The method for preparing a radio-frequency stimulation surgical suture for promoting peripheral nerve healing according to claim 2, characterized in that: The Mo core layer has a diameter of 30-70 μm.

6. The method for preparing a radio-frequency stimulation surgical suture for promoting peripheral nerve healing according to claim 2, characterized in that: The process parameters of the wet spinning are as follows: single-hole nozzle, hole diameter 140-160 μm, and drafting speed 5-8 r / min.

7. The method for preparing a radio-frequency stimulation surgical suture for promoting peripheral nerve healing according to claim 2, characterized in that: The coagulation bath is 90-98% ethanol, and the draft ratio is 1-1.

2.

8. An application of the radio stimulation surgical suture for promoting peripheral nerve healing as claimed in claim 1, characterized in that: Used for repair of peripheral nerve damage.

9. The use of a radio-stimulation surgical suture for promoting peripheral nerve healing according to claim 8, characterized in that: The peripheral nerve injuries include facial nerve, brachial plexus and sciatic nerve.

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