Spathiphyllum hydroponic moss inhibitor and preparation method and detection method thereof

Abstract

The invention provides a spathiphyllum hydroponic moss inhibitor, which comprises a natural plant extract, sodium chloride, acetic acid, biological enzyme and one of copper sulfate or bromocyanoacetamide, the use concentration of the natural plant extract is 2-3.2 ml / L, the use concentration of the copper sulfate is 4-16 mg / L, the purity of the bromocyanoacetamide is 99%, the use concentration is 5 mg / L, and the use concentration of the biological enzyme is 1-2 mg / L. The natural plant extract is cinnamon oil, acetic acid or tea tree oil. CuSO4, LDBNPA (99%) and EM bacteria are finally and successfully proportioned and mixed, so that moss breeding can be inhibited on the premise that normal growth and development of spathiphyllum are guaranteed. The nutrient solution can be used for inhibiting moss breeding when spathiphyllum is hydroponically cultured at home and a large number of spathiphyllum commercial seedlings are hydroponically cultured in factories, so that the time cost can be better saved, and the interval time for replacing the nutrient solution is prolonged.

Description

Technical Field

[0001] The present invention relates to the technical field of hydroponics of plants, and particularly to a moss inhibitor for hydroponics of Spathiphyllum, a preparation method thereof, and a detection method thereof. Background Art

[0002] Hydroponics is a Spathiphyllum cultivation technology that has been studied and applied more in recent years and is currently approaching perfection. However, since hydroponics cultivates plants in an exposed environment and the nutrient solution used contains various nutrient elements, under light conditions, the nutrient solution is prone to produce some moss, which greatly reduces the ornamental value of the flowers. The large reproduction of moss will also deteriorate the growth environment (nutrient solution) of the flower roots, affect the growth of the plants, and compete with hydroponic flowers for nutrients, reducing the oxygen content in the water, resulting in root rot and death of hydroponic flowers, affecting the hydroponic planting industry. And achieving the purpose of removing moss by replacing the nutrient solution often consumes more manpower and material resources. Therefore, a moss inhibitor for hydroponics of Spathiphyllum, a preparation method thereof, and a detection method thereof are proposed to solve the above problems. Summary of the Invention

[0003] In view of the deficiencies of the prior art, the present invention provides a moss inhibitor for hydroponics of Spathiphyllum, a preparation method thereof, and a detection method thereof, which has the advantage of good use effect.

[0004] To achieve the above object, the present invention provides the following technical solution: A moss inhibitor for hydroponics of Spathiphyllum, comprising a natural plant extract, sodium chloride, acetic acid, biological enzyme, and one of copper sulfate or bromoacetonitrile.

[0005] Further, the use concentration of the natural plant extract is 2 - 3.2 ml / L, the use concentration of copper sulfate is 4 mg / L - 16 mg / L, the purity of bromoacetonitrile is 99%, and the use concentration is 5 mg / L.

[0006] Further, the natural plant extract is cinnamon oil, acetic acid or tea tree oil.

[0007] Further, the biological enzyme is nitrifying bacteria or EM bacteria.

[0008] The present invention also provides a preparation method of a moss inhibitor for hydroponics of Spathiphyllum:

[0009] S1. Add 0.05% of the natural plant extract to 1 liter of water, and add a few drops of dishwashing liquid as an emulsifier to make it evenly mixed, and add 1 - 2 ml of acetic acid to each liter of the solution;

[0010] S2. Select a suitable carrier material, mix the biological enzyme suspension with the carrier material to make the biological enzyme adsorb on the surface of the carrier, and centrifuge after mixing the biological enzyme cultured to the logarithmic growth phase with the solution obtained in step 1;

[0011] S3, adding activated carbon powder at a ratio of 0.5-1% to the solution obtained in step 2, stirring and mixing, and then adding 2-2.5% sodium chloride solution dropwise;

[0012] S4. The obtained mixed solution is placed in a 40 kHz ultrasonic processor for 10 minutes to obtain a moss inhibitor.

[0013] The present invention also provides a method for detecting moss grown in hydroponics of Spathiphyllum:

[0014] S1. Stir the fixed volume liquid to be tested evenly with a glass rod;

[0015] S2. Use a pipette to draw a certain volume of nutrient solution and add it to a 1 cm quartz cuvette. Use pure water as a reference and measure its absorbance A value at a wavelength of 672 nm using a T6 New Century UV-Vis spectrophotometer. After measuring the same sample three times, take the average of the three times to obtain a value of one.

[0016] S3, stir the other sample solution evenly with a glass rod and place 10 ml of the solution in a centrifuge tube for later use;

[0017] S4. Place a 0.45 μm water-based microporous filter membrane (mixed fiber) on the Buchner funnel of the filtration device, pour the taken nutrient solution into the Buchner funnel for filtration, and try to drain the water;

[0018] S5. Cut the dried filter membrane into pieces with scissors and put it into a mortar. Add about 5 ml of 90% acetone and grind it thoroughly. Then put the obtained extract into a refrigerator at 4°C and let it stand for 24 hours.

[0019] S6. After standing, use 90% acetone to make the volume 10 ml, put it into a centrifuge and centrifuge it at 3000-4000 r / min for 10 minutes;

[0020] S7. After centrifugation, use a pipette to draw the supernatant into a 1cm optical path quartz cuvette, and read the absorbance at 750nm, 663nm, 645nm, and 630nm on a UV-visible spectrophotometer. Take the average value after measuring the same sample 5 times, and use 90% acetone as a reference. Substitute the measured results into the chlorophyll a calculation formula to calculate its content and obtain value 2, which is:

[0021]

[0022] Where: D—absorbance

[0023] V1—extraction volume (ml)

[0024] V—sample volume (ml)

[0025] Q—the optical path of the cuvette (cm);

[0026] S8. Stir the remaining aliquot of the sample solution to be determined after volume fixation evenly with a glass rod. Use a pipette gun to aspirate one drop and place it on a hemocytometer (25*26 grid network), cover it with a cover glass, place it under a microscope for observation. After recording the number of mosses in 80 grids, substitute it into the calculation formula to obtain Value Three. The formula is:

[0027]

[0028] where: a—the number of mosses in 80 grids;

[0029] S9. Add the three values and take the average to obtain the accurate algal biomass.

[0030] Compared with the prior art, the technical solution of the present application has the following beneficial effects:

[0031] 1. The moss inhibitor for hydroponic Spathiphyllum has the effect of inhibiting moss growth while ensuring the normal growth and development of Spathiphyllum. It can inhibit moss growth in the nutrient solution when hydroponically cultivating Spathiphyllum at home and when mass-producing commercial seedlings of Spathiphyllum in factories, which can better save time costs and increase the interval time for replacing the nutrient solution.

[0032] 2. The preparation method of the moss inhibitor improves the dispersion effect by ultrasonic processor to increase the van der Waals force between additive molecules, making the components mix more evenly.

[0033] 3. The moss detection method detects moss in three directions respectively and takes the average value to obtain a more accurate result for detecting algae. Detailed Embodiments

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below.

[0035] A moss inhibitor for hydroponic Spathiphyllum in the present application includes natural plant extracts, sodium chloride, acetic acid, biological enzymes, and one of copper sulfate or bromoacetonitrile amide.

[0036] Acetic acid is an effective moss inhibitor with less impact on hydroponic plants and will not seriously damage hydroponic plants while inhibiting moss.

[0037] Sodium chloride (table salt) can inhibit moss growth in appropriate amounts and has no negative impact on most hydroponic plants.

[0038] Biological enzymes (such as nitrifying bacteria or EM bacteria): Adding a certain amount of beneficial microorganism solution to the hydroponic system can consume the nutrients required by algae.

[0039] Natural plant extracts: Certain plant extracts have antifungal and antibacterial properties or can change the pH of water, which has an inhibitory effect on moss. At the same time, they are also friendly to hydroponic plants.

[0040] Dilute the above components with water to ensure that they will not cause serious damage to hydroponic plants.

[0041] Furthermore, the concentration of the natural plant extract used is 2 - 3.2 ml / L, the concentration of copper sulfate used is 4 mg / L - 16 mg / L, the purity of bromocyanacetamide is 99%, and the concentration of use is 5 mg / L.

[0042] Furthermore, the natural plant extract is cinnamon oil, acetic acid or tea tree oil.

[0043] Furthermore, the bioenzyme is nitrifying bacteria or EM bacteria.

[0044] The present invention also provides a preparation method of a moss inhibitor for hydroponic Spathiphyllum:

[0045] S1. Add 0.05% of the natural plant extract to 1 liter of water, and add a few drops of dishwashing liquid as an emulsifier to make it evenly mixed. Add 1 - 2 ml of acetic acid to each liter of the solution;

[0046] S2. Select a suitable carrier material, mix the bioenzyme suspension with the carrier material so that the bioenzyme is adsorbed on the surface of the carrier. Mix the bioenzyme cultured to the logarithmic growth phase with the solution obtained in step 1 and then centrifuge;

[0047] S3. Add activated carbon powder to the solution obtained in step 2 in a ratio of 0.5 - 1%, stir well and then add 2 - 2.5% sodium chloride solution drop by drop;

[0048] S4. Place the obtained mixed solution in a 40 kHz ultrasonic processor and process it for 10 minutes to obtain the moss inhibitor.

[0049] The present invention also provides a moss detection method for hydroponic Spathiphyllum:

[0050] S1. Stir the fixed - volume test solution evenly with a glass rod;

[0051] S2. Use a pipette to suck a certain volume of nutrient solution into a 1 cm quartz cuvette. Use pure water as a reference, and measure its absorbance A value at a wavelength of 672 nm with a T6 New Century ultraviolet - visible spectrophotometer. After measuring the same sample 3 times, take the average of the three times to obtain value one;

[0052] S3. Stir the other static and fixed - volume test solution evenly with a glass rod, take out 10 ml and place it in a centrifuge tube for standby;

[0053] S4. Place a 0.45 μm aqueous microporous filter membrane (mixed fiber) on the Buchner funnel of the suction filtration device, then pour the taken nutrient solution into the Buchner funnel for suction filtration, and try to suck the water dry;

[0054] S5. Cut the filter membrane with dried water into pieces with scissors and put them into a mortar. Add about 5 ml of 90% acetone, grind thoroughly, and then put the obtained extract into the refrigerator and let it stand at 4°C for 24 hours;

[0055] S6. After standing, make up the volume to 10 ml with 90% acetone, and centrifuge it in a centrifuge at 3000 - 4000 r / min for 10 minutes;

[0056] S7. After centrifugation, use a pipette to aspirate the supernatant into a 1 cm optical path quartz cuvette, and read the absorbance at wavelengths of 750 nm, 663 nm, 645 nm, and 630 nm on an ultraviolet - visible spectrophotometer. Take the average value after measuring the same sample 5 times, and use 90% acetone as the reference. Substitute the measured results into the chlorophyll a calculation formula to calculate its content, and obtain value two. The formula is:

[0057]

[0058] Where: D - absorbance

[0059] V1 - the volume of the extract after volume - making (ml)

[0060] V - the volume of the sample (ml)

[0061] Q - the optical path of the cuvette (cm);

[0062] S8. Stir the other aliquot of the volume - made test solution evenly with a glass rod. Use a pipette to aspirate a drop and place it in a hemocytometer (25*26 grid network), cover it with a cover glass, observe it under a microscope, record the number of moss in 80 grids, and then substitute it into the calculation formula to obtain value three. The formula is:

[0063]

[0064] Where: a - the number of moss in 80 grids;

[0065] S9. Add the three values and take their average to obtain the accurate algal biomass.

[0066] Comparison with the mortality rate after treatment with common antibacterial and herbicidal drugs

[0067]

[0068] Comparison with the detection results of moss growth after treatment with common antibacterial and herbicidal drugs

[0069]

[0070]

[0071] Among the 5 commonly used antibacterial and herbicidal drugs other than this application, 3 have achieved the effect of inhibiting the growth of moss. The inhibition effects of CK and activated carbon are not good. Among them, prometryn and trifluralin have better inhibition effects than streptomycin sulfate, but the mortality rate of spathiphyllum treated with prometryn is the highest, reaching 50%. The leaves of the surviving plants are severely yellowed and there is root rot. The mortality rate of spathiphyllum treated with trifluralin is 22%. There is also yellowing of the leaves and wilting of the leaf tips. As can be seen from the above, prometryn has a toxic effect on the plants, seriously affecting the survival rate of spathiphyllum seedlings. Trifluralin also has deficiencies. Using the inhibitor of this application can achieve the inhibition of moss growth on the premise of ensuring the normal growth and development of spathiphyllum, is easy to use, can better save time costs, and increase the interval time for replacing nutrient solution.

[0072] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A moss inhibitor for hydroponic cultivation of white palm, characterized by: It includes natural plant extracts, sodium chloride, acetic acid, biological enzymes and one of copper sulfate or bromocyanoacetamide.

2. The moss inhibitor for hydroponic cultivation of white palm according to claim 1, characterized in that: The natural plant extract is used at a concentration of 2-3.2 ml / L, the copper sulfate is used at a concentration of 4 mg / L-16 mg / L, and the purity of bromocyanoacetamide is 99% and the concentration is used at 5 mg / L.

3. The moss inhibitor for hydroponic cultivation of white palm according to claim 1, characterized in that: The natural plant extract is cinnamon oil, acetic acid or tea tree oil.

4. The moss inhibitor for hydroponic cultivation of white palm according to claim 1, characterized in that: The biological enzyme is nitrifying bacteria or EM bacteria.

5. A method for preparing a moss inhibitor for hydroponic cultivation of white palm, characterized in that: S1. Add 0.05% natural plant extract to 1 liter of water, add a few drops of dishwashing liquid as an emulsifier, mix well, and add 1-2 ml of acetic acid per liter of solution; S2, selecting a suitable carrier material, mixing the biological enzyme suspension with the carrier material, allowing the biological enzyme to be adsorbed on the surface of the carrier, mixing the biological enzyme cultured to the logarithmic growth phase with the solution obtained in step 1 and centrifuging; S3, adding activated carbon powder at a ratio of 0.5-1% to the solution obtained in step 2, stirring and mixing, and then adding 2-2.5% sodium chloride solution dropwise; S4. The obtained mixed solution is placed in a 40 kHz ultrasonic processor for 10 minutes to obtain a moss inhibitor.

6. A method for detecting moss grown in hydroponics of Spathiphyllum spathiphyllum, characterized in that: S1. Stir the fixed volume liquid to be tested evenly with a glass rod; S2. Use a pipette to draw a certain volume of nutrient solution and add it to a 1 cm quartz cuvette. Use pure water as a reference and measure its absorbance A value at a wavelength of 672 nm using a T6 New Century UV-Vis spectrophotometer. After measuring the same sample three times, take the average of the three times to obtain a value of one. S3, stir the other sample solution evenly with a glass rod and place 10 ml of the solution in a centrifuge tube for later use; S4. Place a 0.45 μm water-based microporous filter membrane (mixed fiber) on the Buchner funnel of the filtration device, pour the taken nutrient solution into the Buchner funnel for filtration, and try to drain the water; S5. Cut the dried filter membrane into pieces with scissors and put it into a mortar. Add about 5 ml of 90% acetone and grind it thoroughly. Then put the obtained extract into a refrigerator at 4°C and let it stand for 24 hours. S6. After standing, use 90% acetone to make the volume 10 ml, put it into a centrifuge and centrifuge it at 3000-4000 r / min for 10 minutes; S7. After centrifugation, use a pipette to draw the supernatant into a 1cm optical path quartz cuvette, and read the absorbance at 750nm, 663nm, 645nm, and 630nm on a UV-visible spectrophotometer. Take the average value after measuring the same sample 5 times, and use 90% acetone as a reference. Substitute the measured results into the chlorophyll a calculation formula to calculate its content and obtain value 2, which is: Where: D—absorbance V1—extraction volume (ml) V—sample volume (ml) Q—optical path of cuvette (cm); S8. Stir the remaining volume of the test solution with a glass rod, use a pipette to take a drop and place it in a blood cell counting plate (25*26 square grids), cover it with a cover glass, and observe it under a microscope. After recording the number of mosses in 80 squares, substitute it into the calculation formula to obtain the value three, which is: Among them: a—the number of mosses in 80 squares; S9. Add the three values ​​and take the average to get the accurate algae biomass.

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