Sample analysis system and sample analysis method

Abstract

According to the sample analysis system and the sample analysis method provided by the embodiment of the invention, the image acquisition device can obtain the sample image of the to-be-detected sample, the optical detection device can obtain the photoelectric data of the reaction liquid prepared by the to-be-detected sample, and the control device obtains the state information of the to-be-detected sample by analyzing the sample image; photoelectric data of the reaction liquid are analyzed to obtain state information of the reaction liquid, and specific interferent information is determined by integrating the state information of the two aspects, so that a basis is provided for interference elimination, and the accuracy of a sample detection result is improved.

Description

[0001] This application claims the priority of a Chinese patent application titled "Sample Analysis System, Sample Analysis Method" with the application number 202311833928.3, which was filed with the Chinese Patent Office on December 27, 2023. The entire content of this Chinese patent application is incorporated herein by reference. Technical Field

[0002] This application relates to the technical field of medical devices, and more specifically, to a sample analysis system and a sample analysis method. Background Art

[0003] Sample analysis systems such as biochemical analyzers are instruments used to analyze and determine samples. Generally, a reagent is added to the sample to obtain a reaction solution, and the detection results of the reaction solution under various detection items are measured by a certain method. However, in clinical tests, interference caused by the reaction process and the sample to be tested is very common, and these abnormal interferences will affect the accuracy of the detection results. Summary of the Invention

[0004] In view of this, this application provides a sample analysis system and a sample analysis method to determine the information of interfering substances during the sample detection process, provide a basis for interference elimination, and thereby improve the accuracy of the sample detection results.

[0005] In a first aspect, an embodiment of this application provides a sample analysis system, including:

[0006] A sample preparation device configured to prepare a reaction solution using at least a part of the sample to be tested and a reaction reagent;

[0007] A detection device including a light source assembly and an optical signal detector; the light source assembly is configured to generate irradiation light, the irradiation light forms first outgoing light after irradiating the reaction solution, and the optical signal detector collects the first outgoing light to obtain optoelectronic data;

[0008] An image acquisition device configured to acquire a sample image of the sample to be tested;

[0009] A control device configured to determine first state information of the sample to be tested according to the sample image of the sample to be tested, obtain second state information of the reaction solution and the detection result of the detection item of the sample to be tested according to the optoelectronic data, and determine the interference substance information of the reaction solution by combining the first state information and the second state information.

[0010] In a second aspect, an embodiment of this application provides a sample analysis system, including:

[0011] A sample preparation device configured to prepare a reaction solution using at least a part of the sample to be tested and a reaction reagent;

[0012] A detection device, the detection device includes a light source assembly and an optical signal detector; the light source assembly is used to generate irradiation light, the irradiation light forms first outgoing light after irradiating the reaction solution, and the optical signal detector collects the first outgoing light to obtain optoelectronic data;

[0013] An image acquisition device for obtaining a sample image of a sample to be tested in the sample container;

[0014] A control device configured to determine first state information of the sample to be tested according to the sample image of the sample to be tested, obtain second state information of the reaction solution according to the optoelectronic data, correct the optoelectronic data by combining the first state information and the second state information, and obtain a detection result of a detection item of the sample to be tested based on the corrected optoelectronic data.

[0015] In a third aspect, an embodiment of the present application provides a sample analysis system, including:

[0016] A sample preparation device for preparing a reaction solution by using at least part of the sample to be tested and a reaction reagent;

[0017] A detection device for detecting the sample to be tested to obtain a first detection parameter, and detecting the reaction solution to obtain a second detection parameter, where the first detection parameter is different from the second detection parameter;

[0018] A control device configured to obtain first state information of the sample to be tested according to the first detection parameter; obtain second state information of the reaction solution according to the second detection parameter; and determine interference substance information of the reaction solution by combining the first state information and the second state information.

[0019] In a fourth aspect, an embodiment of the present application provides a sample analysis system, including:

[0020] A sample preparation device for preparing a reaction solution by using at least part of the sample to be tested in a sample tube and a reaction reagent;

[0021] A detection device, the detection device includes a light source assembly and an optical signal detector; the light source assembly is used to generate irradiation light, the irradiation light forms first outgoing light after irradiating the reaction solution, and the optical signal detector collects the first outgoing light to obtain optoelectronic data;

[0022] An image acquisition device for acquiring an image of the sample tube of the sample to be tested;

[0023] A control device is configured to obtain first interferent information caused by the sample tube based on an image of the sample tube, obtain second interferent information of the reaction solution and a detection result of a detection item of the sample to be tested based on the optoelectronic data, and determine a source of interferents for the detection item by combining the first interferent information and the second interferent information.

[0024] In a fifth aspect, an embodiment of the present application provides a sample analysis method, including:

[0025] Obtain first status information of the sample to be tested;

[0026] Obtain optoelectronic data of a reaction solution prepared from the sample to be tested and a reagent, and obtain second status information of the reaction solution based on the optoelectronic data, where the optoelectronic data is obtained by collecting light emitted from the reaction solution irradiated by a collection light source;

[0027] Determine interferent information of the reaction solution based on the first status information and the second status information; or, correct the optoelectronic data of the reaction solution based on the first status information and the second status information to obtain a detection result of a detection item.

[0028] In a sixth aspect, an embodiment of the present application provides a sample analysis method, including:

[0029] Obtain a sample image of the sample to be tested;

[0030] When it is determined based on the sample image of the sample to be tested that there are interferents in the sample to be tested, process the sample to be tested to remove the interferents in the sample to be tested;

[0031] Prepare a reaction solution from the processed sample to be tested and a reagent, and obtain a detection result based on the optoelectronic data of the reaction solution, where the optoelectronic data is obtained by collecting light emitted from the reaction solution irradiated by a collection light source.

[0032] Based on the above various technical solutions, in the sample analysis system and the sample analysis method provided by the embodiments of the present application, on the one hand, status information of the sample to be tested is obtained for the sample to be tested, and on the other hand, a reaction solution can be prepared from the sample to be tested and status information of the reaction solution is obtained based on the optoelectronic data of the reaction solution. Thus, interferent information of the reaction solution is comprehensively determined through the status information of the sample to be tested and the status information of the reaction solution. Compared with the interference determination method of manually checking the sample status, the embodiments of the present application can eliminate the influence of subjective factors and the information used for judging interference is more diverse and comprehensive, thereby providing a more reliable basis for interference elimination and further improving the accuracy of the sample detection result. Description of the Drawings

[0033] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0034] Figure 1 A structural schematic diagram of a sample analysis system provided by an embodiment of the present application;

[0035] Figures 2A - 2B Two example diagrams of the optical signal pulse waveform provided by an embodiment of the present application;

[0036] Figure 3A A structural schematic diagram of a single sample analysis device provided by an embodiment of the present application

[0037] Figure 3B A structural schematic diagram of a pipeline system provided by an embodiment of the present application;

[0038] Figure 4 A flowchart of the self-update of the second machine learning model provided by an embodiment of the present application;

[0039] Figure 5 A flowchart of a sample analysis method provided by an embodiment of the present application;

[0040] Figures 6A - 6B Two flowchart diagrams for correcting the detection results of items provided by an embodiment of the present application;

[0041] Figure 7A An example spectrogram of the CK item without interference provided by an embodiment of the present application;

[0042] Figure 7B An example spectrogram of the CK item under the interference of lipemia provided by an embodiment of the present application;

[0043] Figures 8A - 8B Two flowchart diagrams for retesting samples provided by an embodiment of the present application;

[0044] Figure 9 A flowchart of a sample analysis method provided by an embodiment of the present application;

[0045] Figure 10 A flowchart of a sample analysis method provided by an embodiment of the present application. Detailed implementation manners

[0046] In the clinical testing of samples, there are often interferences in the samples themselves or during the reaction process, which affect the accuracy of the test items. Interferences can come from various aspects, including but not limited to: high-concentration environmental CO2 changing the alkaline environment of the reaction, physical collision during blood collection causing physical hemolysis, and improper blood collection by patients resulting in lipemia, etc. Currently, the method of manually observing samples is usually used to exclude interferences, but this method of interference exclusion is affected by subjective factors or due to judgment based on single-factor aspects, resulting in inaccurate determination of interferences. If the information of the interfering substances cannot be determined, corresponding treatment measures cannot be taken to exclude interferences. However, improper abnormal treatment methods not only cannot eliminate interferences but may also cause reagent waste, affect the sample testing throughput and overall efficiency, etc.

[0047] The embodiment of the present application provides a sample analysis system, which comprehensively utilizes the image of the sample to be tested and the optoelectronic data of the reaction solution prepared from the sample to be tested to determine the information of the interfering substances, providing an accurate basis for interference exclusion.

[0048] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0049] The embodiment of the present application provides a sample analysis system, which may include, but is not limited to, any one of the following: a biochemical analysis system, a coagulation analysis system. The sample analysis system includes a sample preparation device, a detection device, an image acquisition device, and a control device.

[0050] The sample preparation device is used to prepare a reaction solution by using at least part of the sample to be tested and a reaction reagent. See Figure 1 As shown in the structural example of a sample analysis system provided, the sample preparation device may include a sample dispensing mechanism 10, a reagent dispensing mechanism 20, and a reaction device 30.

[0051] The sample dispensing mechanism 10 is used to dispense part of the sample to be tested in the sample container into the reaction container. The sample to be tested may include, but is not limited to, any one of a blood sample, a body fluid sample, and a urine sample. In one embodiment, the sample dispensing mechanism at least includes a sampling needle to aspirate the sample to be tested from the sample container and dispense the aspirated sample to be tested into the reaction container of the reaction device. For example, Figure 1The sampling needle of the middle sample dispensing mechanism 10 aspirates a part of the sample to be tested from the sample tube A and dispenses it into the reaction vessel 301. In one embodiment, the sampling needle of the sample dispensing mechanism 10 moves in two or three dimensions spatially through a two-dimensional or three-dimensional driving mechanism to achieve: moving to aspirate the sample to be tested in the sample container and moving to the reaction vessel 301 to be loaded with the sample and discharging the sample into the reaction vessel 301.

[0052] The reagent dispensing mechanism 20 is used to dispense the reaction reagent into the reaction vessel. In one embodiment, the reagent dispensing mechanism 20 may include a reagent needle, and the reagent needle moves in two or three dimensions spatially through a two-dimensional or three-dimensional driving mechanism, which can achieve: moving to aspirate the reagent carried by the reagent component and moving to the reaction vessel 301 to be loaded with the reagent, and discharging the reagent into the reaction vessel 301.

[0053] It should be noted that when preparing the reaction solution in each embodiment of the present application, the dispensing order of the reagent and the sample to be tested into the reaction vessel is not limited, that is: the reagent can be dispensed into the reaction vessel first and then the sample to be tested, or of course, the sample to be tested can be dispensed into the reaction vessel first and then the reagent.

[0054] The reaction device 30 is provided with at least one reaction position for placing the reaction vessel 301 carrying the reaction solution, and the reaction solution is prepared from the sample to be tested and the reagent. Reaction vessels such as reaction vessels, reaction tanks, etc. In one embodiment, the reaction device 30 can be a reaction disk, which is arranged in a disk-shaped structure and has one or more reaction positions for placing reaction vessels. The reaction disk can rotate and drive the reaction vessels in its reaction positions to rotate, for scheduling the reaction vessels in the reaction disk and incubating the reaction solution in the reaction vessels. In one embodiment, the sample analysis system further includes a mixing mechanism for mixing the reaction solution that needs to be mixed in the reaction vessel to make the substances in the reaction solution evenly distributed.

[0055] The detection device includes a light source component and a light signal detector; the light source component is used to generate irradiation light, and the irradiation light forms a first outgoing light after irradiating the reaction solution, and the light signal detector collects the first outgoing light to obtain optoelectronic data. For a structure of the detection device, see Figure 1 As shown, the detection device 40 includes a light source component 401 and a light signal detector 402; the light source component 401 is used to generate irradiation light, and the irradiation light forms an outgoing light after irradiating the reaction solution in the reaction vessel 301, and the light signal detector 402 collects the outgoing light to obtain optoelectronic data. For the convenience of distinguishing from the outgoing light of the reaction reagent, this outgoing light can be called the first outgoing light. In one embodiment, the light signal detector 402 can be a photometer.

[0056] After the reagent in the reaction vessel 301 is mixed with the sample to be tested, a reaction solution can be prepared, and the optoelectronic data obtained in this case is the optoelectronic data of the reaction solution. It should be noted that, in some embodiments, when the sample to be tested is injected into the reaction vessel 301 but the reagent is not injected, the detection device 40 can collect the optoelectronic data of the sample to be tested. In other embodiments, when the reaction reagent is injected into the reaction vessel 301 but the sample to be tested is not injected, the detection device 40 can collect the optoelectronic data of the reaction reagent, and this optoelectronic data can be referred to as the second optoelectronic data.

[0057] There are various specific implementation manners for the detection device 40 to obtain optoelectronic data.

[0058] One implementation manner is to collect optoelectronic data globally. When the reaction vessel and the light source assembly remain relatively stationary, the emitted light formed after the irradiation light generated by the light source assembly 401 irradiates the same position of the reaction solution in the reaction vessel includes at least two light signal of different wavelengths, and the optical signal detector 402 converts the at least two light signals of different wavelengths into optoelectronic data. For example, light signals of multiple wavelengths are collected in parallel at the same time, the detection positions and time periods of the multiple wavelengths are the same, and the optoelectronic data between the multiple wavelengths has reference significance. For example, the state information of the reaction solution can be obtained.

[0059] Another implementation manner is to collect optoelectronic data all the time. The reaction vessel 301 moves relative to the light source assembly 401, and optoelectronic data is collected during the process that the light source assembly 401 continuously irradiates the reaction vessel 402. The optoelectronic data is a signal sequence formed by the relationship between the intensities of at least two light signals and the acquisition time. In some embodiments, the signal sequence can also be referred to as the optical signal pulse waveform. The signal sequence can be a graph including the light signal intensity and time information formed by one pulse, or a data array including the corresponding relationship between the light signal intensity and time information.

[0060] During the process that the light source assembly 401 continuously irradiates the same reaction vessel 402, optoelectronic data of wavelengths is collected in one or more detection cycles. The optoelectronic data of wavelengths includes signal sequences of one or more detection cycles, and the signal sequence of each detection cycle includes at least two light intensity data of this wavelength. Figure 2A For example, the optoelectronic data includes signal sequences of two detection cycles, and the signal sequence of each detection cycle is the corresponding relationship between at least two light intensity signals and the acquisition time.

[0061] Another specific implementation manner is to collect optoelectronic data globally all the time, that is, the combination of the above two collection methods. Light signals of at least two wavelengths are collected all the time to obtain optoelectronic data. In this case, the optoelectronic data obtained can be a signal sequence formed by the relationship between the intensities of light signals of at least two wavelengths and the acquisition time.

[0062] Specifically, during the process of the light source assembly continuously irradiating the same reaction vessel, photoelectric data of n wavelengths are collected in parallel during one or more detection cycles. The photoelectric data of each wavelength includes a signal sequence of one or more detection cycles, and the signal sequence of each detection cycle includes at least two light intensity data of that wavelength. For Figure 2B example, the detection device collects all-time photoelectric data of multiple wavelengths in two detection cycles of the reaction vessel in parallel. The photoelectric data includes signal sequences of multiple wavelengths from wavelength 1 to wavelength n, and each signal sequence includes at least two correspondences between the light signal intensity and the acquisition time. It can also be considered that Figure 2B what is obtained is a multi-wavelength optical signal pulse waveform.

[0063] Another specific implementation method is to use the panoramic dynamic optical measurement technology (Panorama, Dynamic, Recognization, PDR) to collect photoelectric data, that is, to dynamically collect optical signals in the whole time period, the whole wavelength band, and the whole reflection area to obtain the photoelectric data of the reaction solution. When the reaction vessel and the light source assembly remain relatively stationary, the first outgoing light formed by the irradiation light irradiating the same position of the reaction solution in the reaction vessel includes at least two optical signal of different wavelengths. The optical signal detector converts the at least two optical signals of different wavelengths into photoelectric data, and the photoelectric data includes a signal sequence formed by the relationship between the light signal intensities of at least two different wavelengths and the acquisition time. And, when the reaction vessel and the light source assembly rotate relative to each other, the first outgoing light formed by the irradiation light continuously irradiating the reaction solution in the reaction vessel includes at least two optical signals of different wavelengths. The optical signal detector converts the at least two optical signals of different wavelengths into photoelectric data, and the photoelectric data includes a signal sequence formed by the relationship between the light signal intensities of at least two different wavelengths and the acquisition time.

[0064] The image acquisition device 50 is used to acquire a sample image of the sample to be measured in the sample container. In actual application, the sample container can be understood as a transparent or semi-transparent container for loading the sample to be measured. The image obtained by photographing the sample container can reflect the appearance of the sample to be measured. Therefore, this image can be called a sample image. For example, Figure 1 in the image acquisition device 50 can acquire the sample image of the sample to be measured in the sample tube A.

[0065] In one embodiment, the image acquisition device 50 can be a communication interface for receiving a sample image captured by a photographing device such as a camera. In another embodiment, the image acquisition device can include an image acquisition device such as a camera for capturing a sample image. Specifically, the image acquisition device can include a sample holder, a rotating device, and a camera. Among them, the sample holder is used to carry the sample container to keep the sample container in a vertical state, the rotating device is used to drive the sample holder to rotate to drive the sample container carried by the sample holder to rotate around the vertical central axis, and the camera is used to capture the sample image during the rotation of the sample container. In actual application, the duration for the camera to take pictures of the sample container needs to be greater than or equal to the duration required for the sample container to rotate one week or 360 degrees, so as to ensure that during the process of the sample container rotating one week, the camera can obtain images of the sample in the sample container from different shooting angles or perspectives. Of course, in some cases, the image acquisition device does not include a rotating device, and the camera can capture a sample image of a relatively stationary sample container.

[0066] As Figure 3A shown, when the image acquisition device includes an image acquisition device, the sample analysis system can include a single sample analysis device, and the image acquisition device is integrated into the sample analysis device. That is to say, a single sample analysis device can include a sample dispensing mechanism, a reagent dispensing mechanism, a reaction device, a detection device, an image acquisition device, and a control device.

[0067] Or,

[0068] As Figure 3B shown, when the image acquisition device includes an image acquisition device, the sample analysis system includes an assembly line system composed of multiple sample analysis devices connected. The image acquisition device is arranged in the sample loading module of the assembly line system, and the sample loading module is used for the user to place the sample container carrying the sample to be tested. Specifically, the connection of multiple sample analyzers does not necessarily mean that there is a connection between the sample analysis devices. For example, the sample container is transported between each other through a sample transfer unit, or it may also be that the multiple sample analysis devices are independent of each other. The sample transport track of the assembly line system can distribute the sample containers to different sample analysis devices in sequence to represent the connection of multiple sample analysis devices. It should be noted that compared with the sample analysis device shown above Figure 2A shown, the sample analysis device in the assembly line system can include other unit modules (as shown by the dotted box) in addition to the image acquisition device.

[0069] The control device 60 is configured to determine the first state information of the sample to be tested according to the sample image of the sample to be tested, and determine the second state information of the reaction solution according to the optoelectronic data, and jointly determine the interference information of the reaction solution, such as interference substance information, based on the first state information and the second state information. In addition, the control device 60 can at least obtain the test result of the test item of the sample to be tested according to the optoelectronic data.

[0070] The image acquisition device 50 can acquire the sample image of the sample to be tested, the detection device 40 can obtain the optoelectronic data of the reaction solution prepared from the sample to be tested, and the control device 60 can be communicatively connected to the image acquisition device 50 and the detection device 40 to obtain the sample image and the optoelectronic data. On the one hand, the control device 60 analyzes the sample image to obtain the state information of the sample to be tested, and on the other hand, it can analyze the optoelectronic data to obtain the state information of the reaction solution. For the sake of easy distinction, they are respectively referred to as the first state information and the second state information. Among them, the first state information is the information of the interfering substance that causes the sample to be tested to show abnormal traits. The traits include precipitation, color, floccules, lumps, etc. The first state information is used to characterize the interference situation of the sample to be tested (such as whether the sample to be tested is turbid, whether it is hemolyzed, whether it is jaundiced, whether there is a certain type of interfering substance), and the second state information is used to characterize the interference situation of the reaction solution (such as whether the sample to be tested is turbid, whether it is hemolyzed, whether it is jaundiced, whether there is a certain type of interfering substance).

[0071] It can be understood that the reaction solution is prepared from the sample to be tested and the reagent. Therefore, the interference substance information can be determined by analyzing the state difference of the sample to be tested before and after adding the reagent. For example, determine the source of the interfering substance (such as whether it comes from the sample to be tested, whether it comes from the reagent, whether it comes from the reaction process of the reaction after the sample to be tested and the reagent are mixed), and determine the type of the interfering substance (such as the interfering substance type is lipemia interference, hemolysis interference, jaundice interference or other types of interfering substances).

[0072] Since the test results of the test items are obtained based on the optoelectronic data of the reaction solution, but the optoelectronic data is sensitive to some interferences in the reaction solution. For example, abnormalities in the physical properties of the reaction solution such as color, floccules, lumps, precipitates, etc. will cause the optoelectronic data of the reaction solution to be inaccurate, and thus the test results obtained based on the optoelectronic data of the reaction solution are also inaccurate. Therefore, in order to more accurately determine the interference in the reaction solution, the embodiments of the present application comprehensively determine the interference substance information of the reaction solution according to the two aspects of state information, namely the first state information and the second state information. This method can be called joint judgment. Compared with the method of only using the second state information to determine the interference substance information of the reaction solution, the information sources used to judge interference in this joint judgment method are richer, and the judgment results of interference are more accurate. It can be understood that the joint judgment method in the embodiments of the present application does not use only the first state information or only the second state information, but combines two types of state information to obtain the interference substance information of the reaction solution. In addition, from the perspective of the interference substance information, it is the comprehensive judgment result of the two types of state information, namely the first state information and the second state information, rather than being obtained only from one of the types of state information.

[0073] The joint judgment can include various specific implementation methods, which will be described separately below.

[0074] One specific implementation method is to input the first state information and the second state information into a pre-trained artificial intelligence detection model, and the artificial intelligence detection model jointly judges and comprehensively determines the state information of the reaction solution by combining the first state information and the second state information.

[0075] Another specific method is to compare the first state information with the second state information, and determine the type or source of the interfering substance in the reaction solution according to the comparison result. Among them, the first state information is used to reflect the interference related to the sample to be tested, and the second state information is used to reflect the interference related to the reaction solution. By comparing the first state information and the second state information, the differences or similarities between the interferences reflected by the two types of state information can be found, so that the source or type of the interfering substance can be determined.

[0076] In yet another specific comparison method, the first state information and the second state information are signal waveforms (referred to as the first signal waveform and the second signal waveform respectively), and the source of the interfering substance can be determined by comparing the differences between the first signal waveform and the second signal waveform. Specifically, if the first signal waveform is an abnormal signal waveform and the second signal waveform is an abnormal signal waveform, it indicates that the interfering substance is likely to originate from the sample to be tested; if the first signal waveform is a normal signal waveform and the second signal waveform is an abnormal signal waveform, it indicates that the interfering substance is likely to originate from the reaction reagent added to the sample to be tested or from the reaction process; if the first signal waveform is an abnormal signal waveform and the second signal waveform is a normal signal waveform, it indicates that the detection is abnormal or the interfering substance is likely to originate from the sample to be tested; if the first signal waveform is a normal signal waveform and the second signal waveform is a normal signal waveform, it indicates that there is no interfering substance.

[0077] In yet another specific comparison method, the first state information and the second state information are the types of interfering substances (referred to as the first type of interfering substance and the second type of interfering substance respectively), and the type of interfering substance in the reaction solution can be determined by comparing the commonalities and differences between the first type of interfering substance and the second type of interfering substance. For example, the common type of interfering substance in the first type of interfering substance and the second type of interfering substance is determined as the type of interfering substance in the reaction solution; or, if the first type of interfering substance does not include a specific type of interfering substance and the second type of interfering substance includes the specific type of interfering substance, the specific type of interfering substance is excluded from the second type of interfering substance and the remaining second type of interfering substance is used as the type of interfering substance in the reaction solution.

[0078] It can be seen from the above technical solutions that in the sample analysis system provided by the embodiments of the present application, the image acquisition device can obtain a sample image of the sample to be tested, the detection device can obtain the optoelectronic data of the reaction solution prepared from the sample to be tested, the control device analyzes the sample image to obtain the state information of the sample to be tested, analyzes the optoelectronic data of the reaction solution to obtain the state information of the reaction solution, and determines the specific interfering substance information by comprehensively considering the state information of both aspects, thereby providing a basis for interference elimination and improving the accuracy of the sample detection result.

[0079] In some embodiments, when determining the interfering substance information of the reaction solution, the state information of the reaction reagent can be further combined for determination. Specifically, after the reaction reagent is dispensed into the reaction container and before the sample to be tested is dispensed, the irradiation light of the light source assembly irradiates the reaction reagent to form outgoing light. To distinguish it from the outgoing light of the above-mentioned reaction solution, the outgoing light here can be called the second outgoing light. The optical signal detector is also used to collect the second outgoing light and convert the second outgoing light into second optoelectronic data; the control device is also configured to obtain the third state information of the reaction reagent according to the second optoelectronic data.

[0080] Furthermore, the interference information of the reaction solution can be determined by combining the first state information, the second state information, and the third state information. In some embodiments, the common interference types in the first state information, the second state information, and the third state information can be used as the interference information. In some embodiments, if the third state information indicates that the interference source is the sample to be tested or the reaction reagent, the second state information indicates that there is interference in the reaction reagent, but the first state information indicates that there is no interference in the sample to be tested, it can be determined that the interference source is the reaction reagent.

[0081] It can be seen that in this embodiment, the interference information can be determined by combining the state information of the sample to be tested, the state information of the reaction solution, and the state information of the reagent, and the interference determination result is more accurate.

[0082] The following separately describes how to determine the state information (the first state information) of the sample to be tested using the sample image and how to determine the state information (the second state information) of the reaction solution using the optoelectronic data.

[0083] First, the determination method of the first state information is described.

[0084] Method 1: Traditional image analysis algorithm. Use a traditional image feature extraction algorithm to extract at least one of the following types of image features from the sample image: edge features, gray-scale distribution features, color features, texture features, shape features, and spatial relationship features, and obtain the state information of the sample to be tested through the extracted image features. Similarly, the state information of the reaction solution can be obtained. In one case, the state information of the sample to be tested can be directly obtained through at least one type of image feature; in one case, the serum index of the sample to be tested can be first obtained through at least one type of image feature, and then the state information of the sample to be tested can be obtained from the serum index.

[0085] Method 2: Machine learning model analysis. The machine learning model can be pre-trained. Input the sample image into the pre-trained machine learning model, and the machine learning model can automatically output the state information of the sample to be tested. For example:

[0086] 1) Input the sample image into a preset first machine learning model to obtain the first state information of the sample to be tested output by the first machine learning model. 2) Input the sample image into a preset second machine learning model to obtain the serum index of the sample to be tested output by the second machine learning model. For the convenience of distinguishing from the serum index output by the detection device, this serum index can be called the image serum index; and based on the image serum index, the first state information of the sample to be tested is obtained.

[0087] The structure of the first machine learning model and the structure of the second machine learning model may be different or the same. The embodiments of the present application do not limit the structures of the first machine learning model and the second machine learning model. For example, both the first machine learning model and the second machine learning model are neural network models, and may be composed of convolutional neural networks (CNNs, Convolutional Neural Networks).

[0088] Any of the above machine learning models can extract at least one type of image feature from the sample image, such as edge feature, gray scale distribution feature, color feature, texture feature, shape feature, and spatial relationship feature. The edge feature is used to characterize that there are step changes (ramp-type edges) or roof-shaped changes (roof-type edges) in the pixel gray scale, pixel brightness, or pixel color of the image; the gray scale distribution feature characterizes the distribution of the gray scale values of the image. Both the color feature and the texture feature are global features that describe the surface properties of the sample to be measured corresponding to the image or image region. The shape feature includes contour features, which mainly describe the contour or boundary of the label and / or the sample container. The spatial relationship feature refers to the mutual spatial position or relative direction relationship between multiple objects segmented from the image, and these relationships can be divided into connection or adjacency relationships, overlapping or overlapping relationships, inclusion or independent relationships, etc.

[0089] The first machine learning model can use the above at least one type of image to determine the status information of the sample to be measured. When the sample to be measured is a blood sample, the second machine learning model can use the above at least one type of image to determine the serum index of the sample to be measured, and can output the serum index of the sample to be measured. The control device determines the status information of the sample to be measured based on the serum index, or the second machine learning model determines the status information of the sample to be measured using the serum index determined by itself, and thus outputs the status information of the sample to be measured.

[0090] The serum index (HIL) can reflect the status information of the blood sample. Specifically, the blood samples can be divided into the following four types: hemolysis (H, Hemolysis) sample, icterus (I, Icterus) sample, lipemia (L, Lipemia) sample and normal (Normal) sample. Hemolysis, icterus and lipemia (also known as chylomicronemia) are collectively referred to as HIL in the embodiments of the present application. Normal samples can be understood as the presence of an acceptable small amount of hemolysis, icterus and lipemia in serum or plasma, and the color presented by normal samples is translucent yellow or light yellow. Lipemia samples can be understood as the presence of abnormally high concentrations of emulsified fat in blood samples, and the color presented by lipemia samples is dark yellow, gray, white or milky white; the color presented by blood samples is different due to different lipemia indexes. For example, when the lipemia index is low, the blood sample presents a dark yellow color, and when the lipemia index is high, the blood sample presents a gray, white or milky white color. Hemolysis samples can be understood as blood samples in which red blood cells rupture and hemoglobin escapes from red blood cells. The color of hemolysis samples is light red, dark red, or light red to dark red. The higher the hemolysis index, the darker the color of the blood sample. Jaundice samples can be understood as blood samples that are yellowed due to elevated bilirubin. It is precisely because abnormal blood samples will show corresponding color and other characteristics that the status information of the blood sample can be obtained based on the image features. The status information can reflect whether the blood sample is abnormal.

[0091] In order to improve the accuracy of the machine learning model in recognizing the serum index, the serum index of the historical sample can be used to update the machine learning model, specifically, the serum index obtained by comparing the historical samples in two ways (machine learning model recognition and non-machine learning model recognition), and the comparison results are used to update the machine learning model. The specific implementation method is: for each historical sample in multiple historical samples, the first serum index of the historical sample is compared with the second serum index of the historical sample to obtain a serum index comparison result, wherein the first serum index is obtained according to the photoelectric data collected by the optical signal detector after the reaction liquid formed by the historical sample is irradiated by the light source component, and the second serum index is obtained by inputting the sample image of the historical sample into the second machine learning model; the second machine learning model is updated using the serum index comparison results of multiple historical samples.

[0092] like Figure 4As shown, it shows a specific way of self-learning of the second machine learning model. For the same historical sample, on the one hand, an optical detection device can be used to measure its serum index (the light source component irradiates the reaction solution prepared from the historical sample, the optical signal detector collects optoelectronic data, and the optoelectronic data is analyzed to obtain the detection result of the serum index). For the convenience of distinction, this serum index can be called the optically measured serum index. On the other hand, its sample image can be input into the second machine learning model, and after the second machine learning model performs image feature analysis on the sample image, its serum index is output, and this serum index can be called the image serum index. The serum indices obtained by the two methods are compared, and the comparison result is used by the self-learning algorithm to adjust the second machine learning model. It should be noted that the historical sample can be the current sample to be tested, or any sample tested before the sample to be tested. After the second machine learning model is trained, using the historical sample to continuously iterate and optimize the second machine learning model can make the second machine learning model more accurate in identifying the serum index.

[0093] The following describes the method for determining the second state information (the state information of the reaction solution).

[0094] The optoelectronic data of the reaction solution may include the light signal intensities of multiple different wavelengths, and / or include the signal sequence formed by the light signal intensity of the same wavelength and time. By comparing and analyzing the light signal intensities of different wavelengths, and / or analyzing each light signal intensity in the signal sequence of the same wavelength, the state information of the reaction solution is obtained. The state information is used to characterize the interference situation of the reaction solution, and can also be considered to be used to characterize whether the reaction solution is abnormal. For example, it characterizes whether there are foreign substances in the reaction solution, whether the reaction solution is unevenly mixed, whether the reaction solution is clear or turbid, and the specific interfering substances present in the reaction solution (such as lipid particles).

[0095] Method 1: When the control device 60 obtains the second state information of the reaction solution according to the optoelectronic data, it specifically performs the following steps: extracting at least one characteristic interference quantity from the optoelectronic data, and obtaining the second state information of the reaction solution according to the at least one characteristic interference quantity. The characteristic interference quantity is a signal feature used to identify abnormalities.

[0096] Specifically, if the optoelectronic data includes the light signal intensities of multiple wavelengths, the light signal intensities at the same position in the reaction solution at the same time period of multiple wavelengths can be obtained. Since the optoelectronic data between these multiple wavelengths is of reference significance, the state information of the reaction solution can be obtained by comparing the light signal intensities at the same position in the same time period of different wavelengths. The characteristic interference quantities extracted from the light signal intensities of multiple wavelengths include but are not limited to the range of the interference wavelength band.

[0097] If the optoelectronic data includes a signal sequence of the correspondence between the light intensity signal and time at the same wavelength, the signal sequence can be represented as a pulse waveform (or called a signal curve). At least one of the following characteristic interference quantities can be extracted from the optoelectronic data of each wavelength, such as the peak value, valley value, pulse start point, pulse end point, pulse width, full width at half maximum, specific width, area, slope, rise time, and fall time of the signal curve corresponding to the signal sequence. At least one characteristic interference quantity is used to determine the state information of the reaction solution. For example, whether the reaction solution is abnormal can be determined by the number of peaks or valleys of the signal sequence of the same reaction solution in multiple detection cycles.

[0098] The optoelectronic data of the reaction solution can be plotted to obtain a reaction curve (reaction spectrum). Whether the reaction solution is affected by a certain interference can be judged by the reaction curve of the reaction solution and multiple interference characteristic quantities. For example, the interference in the detection item (the detection item does not have a reaction characteristic in the presence of this interference) is identified through a three-dimensional reaction curve. Specifically, if the biochemical reaction of the detection item has a reaction characteristic at wavelengths 1-5, and a certain interference has a reaction characteristic at wavelength 7, then wavelength 7 is considered as the interference characteristic quantity of this interference. When the interference characteristic quantity exceeds the normal threshold, it is considered that the detection item of this sample is affected by this interference.

[0099] Method 2: When the control device 60 obtains the second state information of the reaction solution according to the optoelectronic data, the following steps are specifically executed: The optoelectronic data is input into a preset third machine learning model, and the second state information of the reaction solution output by the third machine learning model for the optoelectronic data is obtained. For example, the absorbance or reactivity can be calculated using the light intensity signal in the optoelectronic data. The absorbance or reactivity is input into the third machine learning model, and the third machine learning model can output whether the reaction solution is abnormal and / or the type of interfering substances in the reaction solution.

[0100] To facilitate the distinction from the above-mentioned first machine learning model and second machine learning model, the machine learning model here can be called the third machine learning model. Similarly to the above-mentioned machine learning models, the third machine learning model can be a neural network model, and its structure can be the same as or different from that of the above-mentioned first machine learning model and second machine learning model. The third machine learning model can be a machine learning model trained with optoelectronic data sample data, so that the third machine learning model has the ability to identify the state information of the reaction solution. Thus, the optoelectronic data can be input into the pre-trained third machine learning model to obtain the state information of the reaction solution. The neural network model can be a network model of any existing structure, including but not limited to a convolutional neural network.

[0101] The interfering substance information of the reaction solution determined by using the first state information and the second state information includes the source of the interfering substance or the type of the interfering substance.

[0102] In some embodiments, the status information of the sample to be tested and the reaction solution may include turbidity values. The source of the interfering substances in the reaction solution can be determined by using the turbidity values between the sample to be tested and the reaction solution. Specifically, the first status information recognized based on the sample image of the sample to be tested includes the turbidity value of the sample to be tested (for the convenience of distinction, referred to as the first turbidity value), and the second status information recognized based on the optoelectronic data of the reaction solution includes the turbidity value of the reaction solution. The turbidity value is used to represent the turbidity degree of the liquid (the sample to be tested or the reaction solution). By comparing the turbidity values of the sample to be tested and the reaction solution (the mixture of the sample to be tested and the reagent), the source of the interfering substances in the reaction solution can be determined.

[0103] In some embodiments, when determining the interfering substance information by using the first status information and the second status information, the control device 60 may specifically be configured to: determine the source of the interfering substances according to the deviation between the first turbidity value and the second turbidity value, such as the deviation between the two turbidity values. Since a normal sample to be tested is usually in a clear state, such as a normal blood / serum sample is in a clear state, and may become turbid if interfered, therefore, by comparing the turbidity value of the reaction solution with the turbidity value of the sample to be tested, if the deviation between the two is large, the source of the interfering substances can be determined. In some embodiments, the status information of the sample to be tested and the reaction solution may include the clear / turbid state, that is, the first status information includes the clear / turbid state of the sample to be tested, and the second status information includes the clear / turbid state of the reaction solution. The source of the interfering substances in the reaction solution can be determined based on the clear / turbid state between the sample to be tested and the reaction solution. The clear / turbid state can be obtained by comparing the turbidity value with a threshold.

[0104] If the turbidity value of the sample to be tested is high and the turbidity value of the reaction solution is also high, it can be determined that the interfering substances come from the sample to be tested; if the turbidity value of the sample to be tested is low but the turbidity value of the reaction solution is high, it can be determined that the interfering substances do not come from the sample to be tested and may come from other interfering factors outside the sample to be tested, such as reagents, sample containers, or the reaction process. The turbidity value can be used to represent the sample characteristics. If the sample characteristics deviate too much, it can be considered that the interfering substances come from the reagents.

[0105] Taking reagents as an example, assume that in the case of a normal and non-interfered blood sample, an abnormal pretreatment solution (such as physiological saline with insufficient salinity) is added to the sample to be tested. Due to the change in the isopotential of the solvent, the proteins originally uniformly distributed in the serum coagulate and precipitate to form chylomicrons, resulting in a certain turbidity of the mixture. The reaction solution further prepared from this mixture will also become turbid. The pretreatment solution includes but is not limited to physiological saline, buffer solution, diluent, etc.

[0106] Taking the reaction process as an example, specific substance components in the sample to be tested may react with specific substance components in the reagent to generate interfering substances. For example, globulin in a blood sample can react with specific substance components in a creatinine reagent to generate substances that cause turbidity, and heparin in a blood sample can react with specific substance components in a creatine kinase reagent to generate substances that cause turbidity.

[0107] Taking the sample container as an example, assuming that there are particulate interferences in the sample container or there are specific substance components that react with the sample to be tested to generate interferences, then the sample container can also be considered as a source of interfering substances.

[0108] In some embodiments, the first status information includes the clarity status of the sample to be tested. The clarity status is a kind of status information used to qualitatively represent the turbidity degree of the sample to be tested, and specifically may include clear or turbid. The second status information includes the clarity status of the reaction solution, and specifically may include clear or turbid. When determining the interfering substance information using the first status information and the second status information, the control device 60 can be used to determine the source of the interfering substance according to the clarity status of the sample to be tested and the clarity status of the reaction solution. The specific process can refer to the related method of the turbidity value above and will not be elaborated here.

[0109] In some embodiments, the first status information includes the clarity status of the sample to be tested. The clarity status is a kind of status information used to qualitatively represent the turbidity degree of the sample to be tested, and specifically may include clear or turbid. The second status information includes the type of interfering substances in the reaction solution. It should be noted that the type of interfering substances included in the second status information can be obtained by any method for detecting interfering substances, including but not limited to spectral detection. Thus, when determining the interfering substance information of the reaction solution according to the first status information and the second status information, the control device 60 is used to: if the clarity status of the sample to be tested is turbid and at least one type of interfering substances in the reaction solution includes lipidemia interference, determine that the type of interfering substance is lipidemia interference. Or, if the clarity status of the sample to be tested is clear, determine the type of interfering substances in the reaction solution according to the type of interfering substances corresponding to the test item of the sample to be tested. For example, taking the type of interfering substances corresponding to the test item as the type of interfering substances in the reaction solution, or excluding lipidemia interference from the type of interfering substances corresponding to the test item to obtain the type of interfering substances in the reaction solution.

[0110] The first status information may be a kind of status identifier. For example, identifier 1 represents turbid and identifier 0 represents clear, or the first status information is status text such as clear or turbid. The second status information may be an identifier of the type of interfering substances. For example, identifier 01 represents interfering substance A, identifier 02 represents interfering substance B, identifier 03 represents interfering substance C, and so on. Or the second status information may also be text of the type of interfering substances.

[0111] Taking a blood sample as an example, if the blood sample is in a turbid state, it indicates that there is lipemia interference in the sample to be tested. At the same time, if the types of interfering substances identified from the reaction solution at least include lipemia interference, then it can be determined that the type of interfering substance is lipemia interference. If the blood sample is in a clear state, it indicates that there is no lipemia interference in the sample to be tested. If the at least one type of interfering substance detected from the reaction solution includes lipemia interference and other types of interfering substances, then lipemia interference can be excluded from the at least one type of interfering substance, so as to obtain an accurate determination result of the type of interfering substance.

[0112] In some embodiments, the optoelectronic data of the reaction solution is obtained during the detection of the item. For example, during the process of the sample analysis system performing a detection item on the sample to be tested, the optoelectronic data of the reaction solution is collected, and the detection result of the item is obtained by analyzing the optoelectronic data. The detection item generally refers to a biochemical detection item.

[0113] Analyzing the optoelectronic data during the detection of the item can, on the one hand, obtain the detection result of the item, and on the other hand, can also obtain the second status information used to indicate whether there is interference in the reaction solution. The second status information includes the types of interfering substances that may exist in the detection item. For example, in the creatinine item, the types of interfering substances that may exist in the analysis of the reaction solution include globulin interference and lipemia interference. In the creatine kinase item, the types of interfering substances that may exist in the analysis of the reaction solution include heparin interference and lipemia interference.

[0114] Combining the representation of the clarity and turbidity state of the sample to be tested in the first status information, and the types of interfering substances that may be included in the second status information, the type of interfering substance in the reaction solution can be accurately determined. Specifically, when determining the interfering substance information of the reaction solution according to the first status information and the second status information, the control device 60 is specifically configured to: combine the clarity and turbidity state of the sample to be tested and the types of interfering substances corresponding to the detection item of the sample to be tested to determine the type of interfering substance in the reaction solution.

[0115] If the clarity and turbidity state of the sample to be tested is clear, then lipemia interference can be excluded from the types of interfering substances corresponding to the detection item. If the clarity and turbidity state of the sample to be tested is turbid and the types of interfering substances corresponding to the detection item of the sample to be tested include lipemia interference, then it can be determined that the type of interfering substance in the reaction solution is lipemia interference.

[0116] Taking the creatinine item as an example, the types of interfering substances included in the second status information include globulin interference and lipemia interference. If the sample to be tested is in a clear state, then it is determined that the type of interfering substance in the reaction solution is globulin interference. If the sample to be tested is in a turbid state, then it is determined that the type of interfering substance in the reaction solution is lipemia interference. Taking the creatine kinase item as an example, the types of interfering substances included in the second status information include heparin interference and lipemia interference. If the sample to be tested is in a clear state, then it is determined that the type of interfering substance in the reaction solution is heparin interference. If the sample to be tested is in a turbid state, then it is determined that the type of interfering substance in the reaction solution is lipemia interference.

[0117] The first status information may include the types of interferents in the sample to be tested. Interferent types of the same nature may be included in the same interferent type group, and the same nature may specifically correspond to the same interference cause, that is, interferent types pointing to the same interference cause may be included in the same interferent type group. Similarly, the second status information may include the interferent type group of the reaction solution. For example, the interference causes of precipitation interference may include lipemia interference or other precipitates. For the convenience of distinction, the two interferent type groups are respectively referred to as the first interferent type group and the second interferent type group.

[0118] When determining the interferent information of the reaction solution according to the first status information and the second status information, the control device 60 is specifically configured to: determine the common interferent types in the first interferent type group and the second interferent type group as the interferent types in the reaction solution. For example, if the first interferent type group includes lipemia interference and the second interferent type group includes lipemia interference and globulin interference, then the common interference type of lipemia interference can be used as the interferent type of the reaction solution.

[0119] See Figure 5 which provides an interference detection process executed by the sample analysis system according to the embodiments of the present application. The sample to be tested is moved to a specified position by the sample transport module (SDM), and the sample to be tested is photographed by the image capturing device to obtain a sample image, and the first status information is identified from the sample image. The reagent dispensing device dispenses the reagent into the reaction vessel, and the sample dispensing device dispenses the sample to be tested into the reaction vessel, and the reaction solution is prepared. The detection device performs optical detection on the reaction solution to obtain optoelectronic data, and the second status information is identified from the optoelectronic data. The first status information and the second status information are compared, and the interferent information is determined according to the comparison result. In the case where the status information is the sample property, if there are significant differences in the sample properties, it can be considered that the interferent comes from the reagent. It should be noted that the reagents added to the reaction vessel may include multiple types, such as pretreatment solution, reaction reagent, etc.

[0120] In some embodiments, the optoelectronic data may be obtained during the detection of the item. Therefore, the control device 60 may also be used to obtain the detection result of the sample to be tested for the detection item according to the optoelectronic data. In a specific implementation manner, the optoelectronic data of the detection item may be used to generate a spectral curve of the detection item, where the abscissa of the spectral curve is the acquisition time and the ordinate is the absorbance or reactivity. The detection result of the detection item can be obtained by analyzing the spectral curve. It should be noted that the spectral curve may be a two-dimensional reaction curve or a three-dimensional spectrogram. In the three-dimensional spectrogram, the optoelectronic data collected by the detection device includes signal sequences of multiple wavelengths. See Figure 7AThe provided normal three-dimensional spectrogram of the creatine kinase (CK) project, where the X-axis in this three-dimensional spectrogram is the acquisition time, the Y-axis is the absorbance, and the Z-axis is the wavelength range of different wavelengths. By analyzing the absorbance at multiple wavelengths in this three-dimensional spectrogram, the detection concentration of creatine kinase in the blood sample can be obtained.

[0121] Since the test sample or the reaction solution prepared from the test sample may have interference, resulting in inaccurate test results obtained from the optoelectronic data of the reaction solution. To improve the accuracy of the test results, the optoelectronic data corresponding to the test item can be corrected to obtain more accurate test results. Therefore, when obtaining the test result of the test sample for the test item based on the optoelectronic data, the control device 60 can specifically be used to: correct the optoelectronic data according to the interference substance information to obtain the test result of the test item. That is to say, the test result obtained by the control device is the corrected test result.

[0122] One correction method can include first using the optoelectronic data of the reaction solution to obtain the initial test result of the test item of the test sample, and then using the interference substance information to correct the initial test result to obtain the test result of the test item of the test item.

[0123] See Figure 6A , in some embodiments, to correct the influence of interference on the test result, the control device 60 is specifically used to execute steps A1 - A2. A1: Determine the initial test result of the test item according to the optoelectronic data corresponding to the detection wavelength; determine the interference amount caused by the interference substance information according to the optoelectronic data corresponding to the interference wavelength; A2: Correct the initial test result with the interference amount to obtain the test result of the test item.

[0124] In this implementation manner, the control device 60 first determines the optoelectronic data corresponding to the detection wavelength of the test item and the optoelectronic data corresponding to the interference wavelength of the interference substance (the interference substance is determined by the interference substance information) in the optoelectronic data. Then, determine the initial test result according to the optoelectronic data corresponding to the detection wavelength. For example, use the optoelectronic data of the detection wavelength to analyze and obtain the test result of the test item (this test result has a deviation from the accurate result due to interference. For the convenience of distinguishing from the corrected test result below, it can be called the initial test result). It can be understood that the test result can represent the proportion of the detected substance corresponding to the test item in the test sample. Similarly, determine the interference amount caused by the interference substance according to the optoelectronic data corresponding to the interference wavelength, that is, use the optoelectronic data of the interference wavelength to determine the test result of the interference substance, that is, the proportion in the test sample. This part can be considered as the proportion of the influence of the interference substance on the test result, so it can be called the interference amount. Finally, correct the initial test result with the interference amount to obtain the test result of the test item, that is, use the interference amount to correct the initial test result, such as subtracting the interference amount from the initial test result to obtain the corrected test result.

[0125] In practical applications, interference cancellation can be performed using an interference calculation formula. The interference calculation formula combines the characteristics of interference optoelectronic data at different time points in different bands. For example, according to the change in the three-dimensional absorbance curve caused by the interfering substance, the influence of interference on the detection result can be eliminated. If the types of interfering substances affecting the detection item are different, different interference calculation formulas corresponding to different types of interfering substances are used for interference cancellation. The interference calculation formula can include spectral information at different wavelengths. For example, one interference calculation formula is x*[a]+y*[b]-z*[c], where x, y, and z are preset coefficients, and a, b, and c are the absorbances at the corresponding wavelengths.

[0126] Taking the creatine kinase (CK) detection item as an example, a reaction solution is prepared using CK reagent. As Figure 7A shown, the normal three-dimensional spectrum of the CK detection item only has spectral peaks below 400 nm, so its main wavelength is 340 nm. Once hemolysis interference exists, see Figure 7B an example diagram of the hemolysis spectrum of the CK detection item provided. Under hemolysis interference, the spectral peak at wavelength 340 nm will be elevated, thus bringing a positive deviation to the detection result. The multi-wavelength interference calculation and processing method is as follows: capture the characteristic peaks brought by interference, especially the characteristic peak at wavelength 412 nm, because normal CK detection does not generate optoelectronic data in this band, and only hemolysis interference has a corresponding strong characteristic peak in this band. Therefore, it is determined whether the hemolysis interference exceeds the acceptance threshold through the spectral information at wavelength 412 nm, and the hemolysis interference is quantified through this wavelength peak. The interference amount brought by hemolysis interference in the spectral information at wavelength 340 nm is calculated through the normalization coefficient formula and eliminated, so as to restore the chemical reaction between the CK detection item and the reagent to the greatest extent and maximize the approximation to the true detection result of the CK detection item. The interference calculation formula corresponding to the hemolysis interference of the CK detection item is x*[absorbance corresponding to 340 nm wavelength]-y*[absorbance corresponding to 412 nm wavelength]. The absorbance after interference cancellation is further converted to obtain the corrected CK detection result. It should be noted that for the CK detection item, other interference characteristic quantities and interference calculation formulas can also be set according to the spectral information.

[0127] Different types of interference have different characteristics and manifestations. The interference calculation formula can quantify the degree of interference of the sample to be measured, so as to realize the correction of the detection result of the detection item.

[0128] Another correction method can include first correcting the optoelectronic data using the interfering substance information of the reaction solution, and then obtaining the detection result of the detection item of the sample to be measured based on the corrected optoelectronic data.

[0129] In some embodiments, when obtaining the detection result of the detection item of the sample to be tested at least based on the optoelectronic data of the reaction solution, the control device 60 is configured to: determine the optoelectronic data corresponding to the detection wavelength of the detection item and the optoelectronic data corresponding to the interference wavelength of the interferent (the interferent is determined by the interferent information) in the optoelectronic data; use the optoelectronic data corresponding to the interference wavelength to correct the optoelectronic data corresponding to the detection wavelength, and obtain the detection result of the detection item based on the corrected optoelectronic data corresponding to the detection wavelength. It can be understood that in the case where the reaction solution is interfered, the optoelectronic data collected by the detection device includes both the optoelectronic data corresponding to the interferent and the optoelectronic data corresponding to the detection item. Therefore, the two types of optoelectronic data are extracted from the optoelectronic data respectively. Specifically, the optoelectronic data includes optoelectronic data of different wavelengths. The wavelengths of the optical signals generated by the interferent and the detection substance corresponding to the detection item may be different. Therefore, the optoelectronic data corresponding to the interferent and the optoelectronic data of the detection item are extracted from the optoelectronic data according to the wavelength band. Furthermore, the optoelectronic data of the detection item is corrected using the optoelectronic data of the interferent to obtain a more accurate detection result.

[0130] See Figure 6B , in some embodiments, to correct the influence of interference on the detection result, the control device 60 is specifically configured to execute steps B1-B2. B1: Correct the optoelectronic data corresponding to the detection wavelength according to the optoelectronic data of the interference wavelength; B2: Obtain the detection result of the detection item according to the corrected optoelectronic data corresponding to the detection wavelength.

[0131] The control device 60 first corrects the optoelectronic data of the detection wavelength, and then analyzes the corrected optoelectronic data of the detection wavelength to obtain the detection result of the detection item, and this detection result is closer to the true result of the sample to be tested. The correction methods of the optoelectronic data of the detection wavelength include but are not limited to: multi-band fitting correction, function correction.

[0132] For example, one way is that if the optoelectronic data of the interference wavelength includes optoelectronic data of multiple wavelengths, the optoelectronic data of the multiple wavelengths are fitted to obtain the interference-fitted optoelectronic data; the interference-fitted optoelectronic data is deducted from the optoelectronic data of the detection wavelength to correct the optoelectronic data of the detection wavelength. Taking the reactivity spectrum as an example, the optoelectronic data of the reaction solution is plotted in a coordinate system to generate the reactivity spectrum. The band data affected by interference in the reactivity spectrum is fitted to obtain the interference spectrum, and the interference spectrum is deducted from the detection wavelength data to obtain a spectrum closer to the interference-free state, and the detection result calculated using this spectrum is more accurate. The absorbance spectrum can be similarly corrected with reference to the above process.

[0133] Suppose the optoelectronic data includes optoelectronic data in bands 1, 2, 3, …, n. According to whether the change in the optical signal intensity of the band is only related to the target interference, part or all of the optoelectronic data in the bands are selected for fitting. For example, the optoelectronic data in bands 1, 2, and m (m ≤ n) are selected, and a specific function is used to fit the responsivity of the selected bands to obtain a fitted spectrum. The optoelectronic data at the detection wavelength is used to generate an original responsivity spectrum. The fitted spectrum is subtracted from the original responsivity spectrum to obtain a corrected responsivity spectrum, and then the corrected responsivity spectrum is used to calculate the detection result of the detection item. The absorbance spectrum can be similarly corrected according to the above process.

[0134] Another method is, in the optoelectronic data at the detection wavelength, N primary-secondary wavelength combinations are selected, and the standard function corresponding to each primary-secondary wavelength combination is determined; the empirical function of the interference caused by the interference substance information to each primary-secondary wavelength combination is selected; for the same primary-secondary wavelength combination, the corresponding standard function and the corresponding empirical function are combined to obtain a superimposed spectral function; the superimposed spectral functions corresponding to the N primary-secondary wavelength combinations are solved to obtain the corrected detection result of the detection item.

[0135] Taking the responsivity spectrum as an example, at least one group of primary-secondary wavelengths is selected. The wavelength combinations of at least one group of primary-secondary wavelengths need to meet the following conditions: the responsivity calculated under wavelength combination 1 is brought into the calibration curve corresponding to this wavelength combination to obtain concentration C1; the responsivity calculated under wavelength combination 2 is brought into the calibration curve corresponding to this wavelength combination to obtain concentration C2; the responsivity calculated under wavelength combination n is brought into the calibration curve corresponding to this wavelength combination to obtain concentration Cn. If multiple concentrations (C1, C2, …, Cn) are close enough or the range is within a small threshold, it means that these multiple wavelength combinations meet the conditions. The absorbance spectrum can be similarly corrected according to the above process.

[0136] It can be understood that the measured responsivity spectrum of each wavelength combination is a linear superposition of the interference spectrum and the target responsivity spectrum. Therefore, after each wavelength combination is selected, a set of equations can be obtained according to the empirical function of the interference spectrum and the calibration curve of the corresponding wavelength combination. The unknowns are the parameters in the empirical function and the concentration. Then, as long as the number of equations in this set of equations is greater than the number of unknowns, the theoretical true concentration can be obtained. The number of equations depends on the number of wavelength combinations, that is, if the number of parameters in the empirical function is N, then the number of required wavelength combinations is N + 1 groups. The absorbance spectrum can be similarly corrected according to the above process.

[0137] To enable the user to know the specific content of the interference information, the control device is further configured to, when the interference information indicates the existence of interference, output interference prompt information according to the interference information, so that the user can eliminate interference based on the interference information. The interference prompt information includes, but is not limited to, whether there is interference, the type of interference source, the source of the interference, the detection items affected by the interference, the impact on the detection results of the detection items (such as positive impact, negative impact, degree of impact), and so on. In some embodiments, the sample analysis system may include a display device, and the control device controls the display device to output the above interference prompt information. The display device may be integrated in a single sample analysis device in the form of a display screen, or may be provided independently of the sample analysis device. For example, in a pipeline system, a pipeline large screen may be set to display the operation status of each sample analysis device. In other embodiments, the sample analysis system may not include a display device, and the control device sends the interference prompt information to the display device for output display. Or the control device is further configured to output the detection results of the detection items, and / or output the interference information of the reaction solution, such as the type of interference source or the source of the interference.

[0138] In some embodiments, if the interference information indicates the existence of an interference source, the sample analysis system can automatically handle the interference: retesting the test sample or retrieving the test sample. The interference handling includes, but is not limited to, handling the interference according to the first status information, or handling the interference according to the interference information obtained from the first status information and the second status information. It should be noted that the interference elimination method performed before retesting is related to the type of interference. For example, if there is lipemia interference, interference can be eliminated by methods such as dilution with a reduced amount. If there is hemolysis interference, blood can be recollected, etc.

[0139] When the interference information indicates the first severity level of the interference degree, eliminate the interference and retest. When the interference information indicates the second severity level of the interference degree, directly retrieve the test sample, such as transporting the sample container to the retrieval position. After obtaining the interference information by combining the first status information and the second status information, using the interference information for interference handling has a higher accuracy of the processing basis than directly using the first status information for automatic processing.

[0140] However, to further save the sample testing time, simplify the repeated steps, and improve the testing efficiency, the test sample can be directly retested or retrieved according to the first status information after obtaining the first status information. The following analyzes two retesting processes by comparison to illustrate the advantages of this retesting method in simplifying the testing process.

[0141] Such as Figure 8A, in a test process, reagent R1, the sample to be tested, and reagent R2 are respectively dispensed into a reaction vessel. After preparing the reaction solution, interference detection is carried out. It is detected that there is lipemia interference in the reaction solution, then the sample to be tested is diluted, and reagent R1, the diluted sample to be tested, and reagent R2 are respectively dispensed into the reaction vessel again. After preparing the reaction solution, item detection is carried out to obtain an interference-free test result.

[0142] For example Figure 8B , in a test process, an image acquisition device obtains a sample image of the sample to be tested, and recognizes the first state information from the sample image. If the first state information indicates the existence of lipemia interference, the sample to be tested is directly diluted, and then reagent R1, the diluted sample to be tested, and reagent R2 are respectively dispensed into the reaction vessel. After preparing the reaction solution, item detection is carried out to obtain an interference-free test result. It can be seen that this test process avoids discovering interference after the test is completed, directly discovers and processes interference before adding reagents, saving test steps and improving test efficiency.

[0143] In specific implementation, if the first state information indicates that the degree of interference of the sample to be tested reaches the first severity level, the sample to be tested is first processed accordingly according to the interference type corresponding to the first sample state, and then the sample dispensing mechanism is controlled to aspirate the sample from the processed sample to be tested and transport it to the reaction vessel, and the reagent dispensing mechanism is controlled to dispense the reagent into the reaction vessel. After preparing the reaction solution in the reaction vessel, the test of the detection item is carried out. If the first state information indicates that the degree of interference of the sample to be tested reaches the second severity level, the sample recovery container is controlled.

[0144] To further improve the recognition accuracy of interference substance information, in some embodiments, interference determination can be combined with the optoelectronic data of the reagent. Specifically, the control device can control the reagent dispensing mechanism to dispense the reagent into the reaction vessel, and obtain the optoelectronic data of the reagent collected by the optical signal detector after the reagent is added to the reaction vessel; control the sample dispensing mechanism to dispense the sample to be tested into the reaction vessel (if the aforementioned reagent is a pretreatment solution, control the reagent dispensing mechanism to inject the reaction reagent into the reaction vessel), and the sample to be tested in the reaction vessel and the reagent are prepared to obtain a reaction solution; determine the interference substance information of the reaction solution according to the first state information of the sample to be tested, the second state information of the reaction solution, and the optoelectronic data of the reagent.

[0145] When there is only reagent in the reaction vessel, the state of the reagent in the reaction vessel such as the pH value of the reagent can be obtained according to the optoelectronic data or the absorbance or transmittance obtained from the optoelectronic data, and whether the reagent is abnormal is determined according to the obtained reagent state, such as the reagent bottle has been opened for too long, the reagent has expired or the reagent has deteriorated, etc.

[0146] The embodiments of the present application further provide a sample analysis system, which can correct the detection results of detection items according to the first state information and the second state information, so that the sample analysis system excludes interference and outputs detection results closer to the true sample situation. The sample analysis system provided by the embodiments of the present application includes: a sample preparation device, a detection device, an image acquisition device, and a control device.

[0147] The sample preparation device is used to prepare a reaction solution by using at least part of the sample to be tested and a reaction reagent. In a specific implementation, the sample preparation device includes a sample dispensing mechanism, a reagent dispensing mechanism, and a reaction device.

[0148] The detection device includes a light source assembly and an optical signal detector; the light source assembly is used to generate irradiation light, and the irradiation light forms a first outgoing light after irradiating the reaction solution, and the optical signal detector collects the first outgoing light to obtain optoelectronic data;

[0149] The image acquisition device is used to obtain a sample image of the sample to be tested in the sample container;

[0150] The control device is configured to determine the first state information of the sample to be tested according to the sample image of the sample to be tested, obtain the second state information of the reaction solution according to the optoelectronic data, jointly correct the optoelectronic data according to the first state information and the second state information, and obtain the detection result of the detection item of the sample to be tested based on the corrected optoelectronic data.

[0151] Only the detection device and the control device are briefly described below. For the descriptions of other parts, reference can be made to the relevant descriptions of the above embodiments, and details are not repeated here.

[0152] It should be noted that the detection device collects optoelectronic data for a specific detection item, that is, for the item to be detected. During the detection process of this item, the light source assembly is used to generate irradiation light, the irradiation light irradiates the reaction container to form outgoing light, and the optical signal detector collects the outgoing light to obtain optoelectronic data.

[0153] The control device is configured to determine the first state information of the sample to be tested according to the sample image of the sample to be tested, and obtain the second state information of the reaction solution according to the optoelectronic data. The first state information and the second state information are jointly used to correct the optoelectronic data of the reaction solution in the detection item, so as to obtain the corrected item detection result. After obtaining the first state information and the second state information, the control device can directly correct the optoelectronic data of the reaction solution in the detection item according to the two state information, so as to obtain the corrected item detection result. In some embodiments, the first state information of the sample to be tested is information about the interfering substance that causes the sample to be tested to have abnormal characteristics.

[0154] In some embodiments, the optoelectronic data of the detection item is corrected according to the interference situation represented by the first status information, and a relatively accurate detection result is obtained by using the corrected optoelectronic data.

[0155] In other embodiments, interference object information is obtained according to the first status information and the second status information; the optoelectronic data is corrected by using the interference object information to obtain the detection result of the detection item. For the specific description of this embodiment, reference can be made to the above relevant description, which will not be elaborated here.

[0156] The embodiment of the present application further provides a sample analysis system, including a sample preparation device, a detection device, an image acquisition device and a control device. Only a brief description of the control device will be given below. For the description of other parts, reference can be made to the relevant descriptions of the above embodiments, which will not be elaborated here.

[0157] The control device is configured to: when it is determined according to the sample image of the sample to be tested that there is an interference object in the sample to be tested, process the sample to be tested to remove the interference object in the sample to be tested; control the sample dispensing mechanism to dispense the processed sample to be tested into the reaction container; and obtain the detection result according to the optoelectronic data of the reaction solution.

[0158] The sample analysis system of this technical solution can first process the sample to be tested to remove the interference object when it is detected that there is an interference object in the sample to be tested. It can be understood that the specific processing process is related to the type of the interference object. After obtaining the sample to be tested with the interference removed, the sample to be tested is then detected to obtain the detection result. One interference processing method is to process the sample to be tested after the interference is found during the project detection, and then re-perform the project detection. Compared with this interference processing method, the sample analysis system of this technical solution can avoid repeated detection, process the interference object before detection, reduce the waste of reagents and other consumables caused by the project detection, and save the detection process.

[0159] The embodiment of the present application further provides a sample analysis system, including a sample preparation device, a detection device and a control device. Compared with the sample analysis systems of the above embodiments, it does not necessarily include an image acquisition device, and the control device can obtain the status information of the sample to be tested sent by other devices. Only a brief description of the control device will be given below. For the description of other parts, reference can be made to the relevant descriptions of the above embodiments, which will not be elaborated here.

[0160] A control device is configured to obtain first state information of a sample to be tested. In some embodiments, the first state information of the sample to be tested is information about an interfering substance that causes an abnormal trait in the sample to be tested, and to obtain second state information of a reaction solution and a test result of a test item of the sample to be tested based on optoelectronic data, and jointly determine interfering substance information of the reaction solution according to the first state information and the second state information. Among them, the first state information can be sent by other devices or obtained by itself.

[0161] The state information of the sample to be tested is used to represent the interference situation of the sample to be tested. In some embodiments, the sample to be tested can be photographed to obtain a sample image, and the state information can be obtained after recognizing the sample image. In some embodiments, the serum index test can be performed on the sample to be tested, and the state information can be obtained through the serum index. In some embodiments, an optical detection device can also be used to obtain optoelectronic data of the sample to be tested, and the state information can be obtained by analyzing the interference characteristic quantity of the optoelectronic data. Of course, other arbitrary interference detection means can also be used to obtain the state information of the sample to be tested.

[0162] An embodiment of the present application also provides a sample analysis system, including: a sample preparation device, a detection device, and a control device.

[0163] The sample preparation device is used to prepare a reaction solution by using at least part of the sample to be tested and a reaction reagent.

[0164] The detection device is used to detect the sample to be tested to obtain a first detection parameter and detect the reaction solution to obtain a second detection parameter.

[0165] Among them, the first detection parameter refers to a parameter obtained by detecting an interfering substance in the sample to be tested, and the first detection parameter is used to obtain the first state information in the sample to be tested.

[0166] For example, the first detection parameter is detected by using image processing technology. Correspondingly, the first detection parameter includes image parameters. The sample to be tested (or a pretreatment solution of the sample to be tested) is photographed to obtain a sample image of the sample to be tested (or a pretreatment solution of the sample to be tested), and image parameters are obtained after analyzing the sample image. The image parameters are used to represent the first state information of the sample to be tested. It can be understood that the image parameters can reflect the physical properties of the sample to be tested (such as color, turbidity, precipitation, lumps, flocs, etc.). The image parameters can include but are not limited to color, pixel depth, resolution, bit depth, hue, saturation, brightness, color channels, etc.

[0167] For another example, the first detection parameter is detected by using an optical analysis technique, and correspondingly, the first detection parameter includes optoelectronic data. The specific process of using the optical analysis technique to detect optoelectronic data can refer to the relevant descriptions of the above light source assembly and optical signal detector, which will not be elaborated here. Further, the optical analysis technique may include PDR technology, and the optoelectronic data includes PDR optoelectronic data.

[0168] Among them, the second detection parameter refers to the parameter detected for the interfering substances in the reaction solution, and the second detection parameter is used to obtain the second state information in the reaction solution.

[0169] For example, the second detection parameter is detected by using an optical analysis technique, then the second detection parameter includes optoelectronic data. In a specific implementation, the detection device includes a light source assembly and an optical signal detector; the light source assembly is used to generate irradiation light, and the irradiation light forms outgoing light after irradiating the reaction solution, and the optical signal detector collects the outgoing light and converts the outgoing light into optoelectronic data; among them, the second detection parameter includes optoelectronic data. In this implementation, the control device is configured to obtain the second state information in the reaction solution according to the optoelectronic data included in the second detection parameter. Further, the optical analysis technique may include PDR technology, and the second optoelectronic data includes PDR optoelectronic data.

[0170] The control device is configured to obtain the first state information in the sample to be tested according to the first detection parameter; obtain the second state information of the reaction solution according to the second detection parameter; combine the first state information and the second state information to determine the interfering substance information of the reaction solution, such as the type of interfering substance or the source of the interfering substance.

[0171] The embodiment of the present application further provides a sample analysis system, including: a sample preparation device, a detection device, an image acquisition device, and a control device. Different from the above embodiments, the image acquisition device is used to acquire an image of the sample tube of the sample to be tested, so that the control device obtains the first interfering substance information of the sample tube according to the image of the sample tube, and the first interfering substance information includes the chemical reagent type of the chemical reagent contained in the sample tube, such as heparin belongs to an anticoagulant, and ethylenediaminetetraacetic acid (EDTA) also belongs to an anticoagulant, etc.

[0172] Specifically, the image acquired by the image acquisition device may include the cap image of the sample tube for carrying the sample to be tested, and / or the label image of the sample tube for carrying the sample to be tested. In practical applications, the sample tube for carrying the sample may include some substances such as chemical reagents, and these chemical reagents may react with the substances in the reaction system such as the sample to be tested and the reaction reagent, thereby interfering with the detection results of the detection items. If it is determined according to the image of the sample tube that it may affect the detection results of certain detection items, then the sample to be tested can be marked and a sample tube alarm can be issued.

[0173] In a specific case, the type of chemical reagent in the sample tube can be determined by the color of the tube cap of the sample tube. For example, the sample tube with a green tube cap is a heparin tube which contains an anticoagulant, and the anticoagulant will cause interference in the test results of items such as α-L-fucosidase (AFU) and creatine kinase (CK). The sample tube with a yellow tube cap contains a clot activator, which may affect the test results of certain test items. In another case, a label is attached to the outer wall of the sample tube and the color of the label is usually the same as that of the tube cap, so the type of chemical reagent in the sample tube can also be determined by the color of the label.

[0174] The control device can also obtain the second state information of the reaction solution based on the optoelectronic data of the reaction solution. The second interferent information includes the types of chemical reagents that may cause abnormal test results of the test items, such as clot activators, anticoagulants, etc. In practical applications, the interference relationship between the test items and the types of chemical reagents can be preset in advance, and through the interference relationship, it can be determined which test items will be affected by which types of chemical reagents.

[0175] Furthermore, by combining the first state information and the second state information, the interferent information of the reaction solution, such as the type of interferent or the source of the interferent, is determined. Specifically, if the first interferent information and the second interferent information include the same type of chemical reagent, it is determined that the source of the interferent of the test item includes the sample tube.

[0176] In a specific embodiment, the test item is the creatine kinase item, and both the first interferent information and the second interferent information include anticoagulants. It is determined that the source of the interferent of the creatine kinase item includes the sample tube. In another specific embodiment, the test item is the α-L-fucosidase item, and both the first interferent information and the second interferent information include anticoagulants. It is determined that the source of the interferent of the α-L-fucosidase item includes the sample tube.

[0177] The embodiment of the present application also provides a sample analysis method, as Figure 9 shown, the sample analysis method includes steps S91-S93.

[0178] S91: Obtain the first state information of the sample to be tested.

[0179] Among them, the state information of the sample to be tested is obtained through various interferent test methods such as sample to be tested image recognition, serum index test, optical detection, etc.

[0180] S92: Obtain the optoelectronic data of the reaction solution prepared from the sample to be tested and the reagent, and obtain the second state information of the reaction solution according to the optoelectronic data. The optoelectronic data is obtained by collecting the outgoing light of the reaction solution irradiated by the light source.

[0181] S93: Determine the interfering substance information of the reaction solution based on the first status information and the second status information; or, correct the optoelectronic data of the reaction solution according to the first status information and the second status information to obtain the test result of the test item.

[0182] In this embodiment, the status information of the test sample and the status information of the reaction solution can be used for interference detection to obtain accurate interfering substance information of the reaction solution. Or, the status information of the test sample and the status information of the reaction solution can also correct the optoelectronic data of the reaction solution, so as to obtain a relatively accurate test result of the item. For specific descriptions, reference can be made to the relevant content above, which will not be elaborated here.

[0183] The embodiment of the present application also provides a sample analysis method, as Figure 10 shown. This sample analysis method includes steps S101 - S103.

[0184] S101: Obtain the sample image of the test sample.

[0185] S102: When it is determined from the sample image of the test sample that there are interfering substances in the test sample, process the test sample to remove the interfering substances in the test sample.

[0186] S103: Prepare a reaction solution by mixing the processed test sample with a reagent, and obtain a test result based on the optoelectronic data of the reaction solution, where the optoelectronic data is obtained by collecting the outgoing light of the reaction solution irradiated by a light source.

[0187] For the sample analysis method of this technical solution, when it is detected that there are interfering substances in the test sample, the test sample can be processed first to remove the interfering substances. It can be understood that the specific processing process is related to the type of interfering substances. After obtaining the test sample with the interfering substances removed, the test sample is then detected to obtain the test result. One interference processing method is to process the test sample after interference is found during the item detection, and then re - perform the item detection. Compared with this interference processing method, the sample analysis method of this technical solution can avoid repeated detection, process the interfering substances before detection, reduce the waste of reagents and other consumables caused by item detection, and save the detection process.

[0188] Regarding the above description of the disclosed embodiments, the features described in each embodiment in this specification can be replaced or combined with each other, enabling those skilled in the art to implement or use this application. This description refers to various exemplary embodiments. However, those skilled in the art will recognize that changes and modifications can be made to the exemplary embodiments without departing from the scope hereof. For example, various operation steps and the components for performing the operation steps can be implemented in different ways according to a specific application or any number of cost functions associated with the operation of the system (e.g., one or more steps can be deleted, modified, or incorporated into other steps).

[0189] In the specification, claims, and the above drawings of this application, the terms "first", "second", etc. are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or units is not limited to the listed steps or units, but optionally further includes steps or units not listed, or optionally further includes other steps or units inherent to these processes, methods, or devices.

[0190] In addition, as understood by those skilled in the art, the principles herein can be embodied in a computer program product on a computer-readable storage medium that is pre-loaded with computer-readable program code. Any tangible, non-transitory computer-readable storage medium can be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-ROMs, DVDs, Blu-ray discs, etc.), flash memories, and / or the like. These computer program instructions can be loaded onto a general-purpose computer, a special-purpose computer, or other programmable data processing devices to form a machine, such that the instructions executed on the computer or other programmable data processing devices can generate a device for implementing the specified functions. These computer program instructions can also be stored in a computer-readable memory, which can direct the computer or other programmable data processing devices to operate in a specific manner, so that the instructions stored in the computer-readable memory can form a manufactured article, including a device for implementing the specified functions. The computer program instructions can also be loaded onto a computer or other programmable data processing devices, thereby performing a series of operation steps on the computer or other programmable devices to generate a computer-implemented process, such that the instructions executed on the computer or other programmable devices can provide steps for implementing the specified functions.

[0191] The foregoing detailed description has been presented with reference to various embodiments. However, those skilled in the art will recognize that various modifications and changes can be made without departing from the scope of this disclosure. Accordingly, the contemplation of this disclosure is illustrative in nature and not restrictive, and all such modifications will be included within its scope. Similarly, the advantages, other advantages, and solutions to problems with respect to the various embodiments have been described above. However, benefits, advantages, solutions to problems, and any elements that produce these, or that make them more explicit, should not be construed as critical, required, or essential. As used herein, the term "comprising" and any other variants thereof are non-exclusive inclusions, such that a process, method, article, or apparatus that includes a list of elements does not include only those elements but also other elements not expressly listed or inherent to the process, method, system, article, or apparatus. Additionally, as used herein, the term "coupled" and any other variants thereof refer to physical connection, electrical connection, magnetic connection, optical connection, communication connection, functional connection, and / or any other connection.

[0192] The above embodiments merely represent several implementation manners, and their descriptions are relatively specific and detailed. However, they should not be construed as limiting the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the invention, several variations and improvements can still be made, and these all fall within the protection scope of the invention. Therefore, the protection scope of the invention patent shall be subject to the appended claims.

Claims

1. A sample analysis system, characterized in that: include: A sample preparation device, the sample preparation device is used to prepare a reaction solution using at least part of the sample to be tested and a reaction reagent; A detection device, the detection device comprising a light source assembly and an optical signal detector; the light source assembly is used to generate irradiation light, the irradiation light forms a first outgoing light after irradiating the reaction liquid, and the optical signal detector collects the first outgoing light to obtain photoelectric data; An image acquisition device, used to acquire a sample image of the sample to be tested; The control device is configured to determine the first state information of the sample to be tested based on the sample image of the sample to be tested, obtain the second state information of the reaction liquid and the detection result of the detection item of the sample to be tested based on the photoelectric data, and determine the interference information of the reaction liquid by combining the first state information and the second state information.

2. The sample analysis system according to claim 1, characterized in that: The sample preparation device comprises: A reaction device, wherein the reaction device is provided with at least one reaction position, and the reaction position is used to place the reaction container; A sample dispensing mechanism, used for dispensing at least a portion of the sample to be tested into the reaction container; A reagent dispensing mechanism, used for dispensing the reaction reagent into the reaction container; The reaction container is used for preparing a reaction solution with the sample to be tested and the reaction reagent; wherein: When the reaction container and the light source assembly remain relatively still, the first emergent light formed by the irradiation light irradiating the same position of the reaction liquid in the reaction container includes at least two light signals of different wavelengths, and / or, when the reaction container and the light source assembly rotate relatively, the first emergent light formed by the irradiation light continuously irradiating the reaction liquid in the reaction container includes at least two light signals of different wavelengths; The optical signal detector is used to convert the at least two optical signals of different wavelengths into the photoelectric data, and the photoelectric data includes a signal sequence formed by the relationship between the intensity of the at least two optical signals of different wavelengths and the acquisition time.

3. The sample analysis system according to claim 1 or 2, characterized in that: The irradiation light forms a second outgoing light after irradiating the reaction reagent, and the optical signal detector is further used to collect the second outgoing light and convert the second outgoing light into second photoelectric data; the control device is also configured to obtain third state information of the reaction reagent according to the second photoelectric data; The combining of the first state information and the second state information to determine the interference information of the reaction liquid includes: combining the first state information, the second state information and the third state information to determine the interference information of the reaction liquid, wherein after dispensing the reaction reagent and before dispensing the sample to be tested, the optical signal detector collects the second output light.

4. The sample analysis system according to claim 1, wherein: The step of determining the first state information of the sample to be tested according to the sample image of the sample to be tested includes: Inputting the sample image into a preset first machine learning model, and obtaining first state information of the sample to be tested output by the first machine learning model; or, The sample image is input into a preset second machine learning model, and the image serum index of the sample to be tested output by the second machine learning model is obtained; and the first state information of the sample to be tested is obtained based on the image serum index.

5. The sample analysis system according to claim 1, wherein: The interference information includes the interference source or the interference type.

6. The sample analysis system according to claim 5, characterized in that: The first state information includes a first turbidity value of the sample to be tested and the second state information includes a second turbidity value of the reaction solution; or, the first state information includes the clarity or turbidity of the sample to be tested and the second state information includes the clarity or turbidity of the reaction solution.

7. The sample analysis system according to claim 5, characterized in that: The first state information includes the clear or turbid state of the sample to be tested, and the second state information includes the type of the interferent in the reaction solution; and the combining of the first state information and the second state information to determine the interferent information of the reaction solution includes: If the turbidity state of the sample to be tested is turbid and at least one type of interference in the reaction solution includes lipemia interference, determining that the type of interference is lipemia interference; or, If the turbidity state of the sample to be tested is clear, the type of the interferent in the reaction solution is determined according to the type of the interferent corresponding to the detection item of the sample to be tested.

8. The sample analysis system according to claim 7, characterized in that: The detection item is a creatinine item, the interference type included in the second state information includes globulin interference and lipemia interference, and the determination of the interference type of the interference in the reaction solution according to the interference type corresponding to the detection item of the sample to be tested includes: determining that the interference type of the reaction solution is globulin interference; or, The detection item is a creatine kinase item, the interference types included in the second state information include heparin interference and lipemia interference, and determining the interference type of the interference in the reaction solution according to the interference type corresponding to the detection item of the sample to be tested includes: determining that the interference type of the reaction solution is heparin interference.

9. The sample analysis system according to claim 5, characterized in that: The first state information includes a first interferent type group of the sample to be tested, and the second state information includes a second interferent type group of the reaction solution; and the combining the first state information and the second state information to determine the interferent information of the reaction solution includes: The common interferent type in the first interferent type group and the second interferent type group is determined as the interferent type of the reaction solution.

10. The sample analysis system according to claim 1, wherein: The obtaining the second state information of the reaction liquid according to the photoelectric data comprises: Extracting at least one characteristic interference quantity from the photoelectric data, and obtaining second state information of the reaction liquid according to the at least one characteristic interference quantity; or, The photoelectric data is input into a preset third machine learning model to obtain second state information of the reaction liquid output by the third machine learning model to the photoelectric data.

11. The sample analysis system according to claim 1, wherein: Obtaining the test result of the test item of the sample to be tested according to the photoelectric data includes: The photoelectric data is corrected according to the interference information of the reaction solution, and the detection result of the detection item of the sample to be detected is obtained based on the corrected photoelectric data.

12. The sample analysis system according to claim 11, characterized in that: The step of correcting the photoelectric data according to the interference information of the reaction solution and obtaining the test result of the test item of the sample to be tested based on the corrected photoelectric data includes: Determine the photoelectric data corresponding to the detection wavelength of the detection item and the photoelectric data corresponding to the interference wavelength of the interference object in the photoelectric data; the interference object is determined by the interference object information; The photoelectric data corresponding to the detection wavelength is corrected according to the photoelectric data corresponding to the interference wavelength, and the detection result of the detection item is obtained based on the corrected photoelectric data corresponding to the detection wavelength.

13. The sample analysis system according to claim 1, wherein: Obtaining the test result of the test item of the sample to be tested according to the photoelectric data includes: An initial detection result of the detection item of the sample to be detected is obtained according to the photoelectric data, and the initial detection result is corrected according to the interference information to obtain the detection result of the detection item of the sample to be detected.

14. The sample analysis system according to claim 13, wherein: The method of using the photoelectric data corresponding to the interference wavelength and the photoelectric data corresponding to the detection wavelength to obtain the detection result after correction of the detection item includes: Determine the photoelectric data corresponding to the detection wavelength of the detection item and the photoelectric data corresponding to the interference wavelength of the interference object in the photoelectric data; the interference object is determined by the interference object information; Determine the initial detection result according to the photoelectric data corresponding to the detection wavelength, and determine the interference amount caused by the interference object according to the photoelectric data corresponding to the interference wavelength; The initial detection result is corrected according to the interference amount to obtain the detection result of the detection item.

15. The sample analysis system according to any one of claims 1 to 14, characterized in that: The control device is further used to: output the detection result, and / or output the interference information.

16. The sample analysis system according to any one of claims 1 to 15, characterized in that: After determining the interference information, the control device is further used to: If the interferer information indicates that an interferer exists, the sample to be tested is retested or the sample to be tested is recovered.

17. The sample analysis system according to any one of claims 1 to 14, characterized in that: The image acquisition device includes an image acquisition device; The sample analysis system includes a sample analysis device, and the image acquisition device is integrated in the sample analysis device; or, the sample analysis system includes an assembly line system composed of a plurality of sample analysis devices connected together, and the image acquisition device is arranged in a sample loading module of the assembly line system, and the sample loading module is used for a user to place a sample container carrying the sample to be tested.

18. The sample analysis system according to any one of claims 1 to 14, characterized in that: The first state information of the sample to be tested is information about an interferent that causes the sample to be tested to have abnormal properties.

19. A sample analysis system, characterized in that: include: A sample preparation device, the sample preparation device is used to prepare a reaction solution using at least part of the sample to be tested and a reaction reagent; A detection device, the detection device comprising a light source assembly and an optical signal detector; the light source assembly is used to generate irradiation light, the irradiation light forms a first outgoing light after irradiating the reaction liquid, and the optical signal detector collects the first outgoing light to obtain photoelectric data; An image acquisition device, used to obtain a sample image of the sample to be tested in the sample container; The control device is configured to determine the first state information of the sample to be tested based on the sample image of the sample to be tested, obtain the second state information of the reaction liquid based on the photoelectric data, correct the photoelectric data in combination with the first state information and the second state information, and obtain the detection result of the detection item of the sample to be tested based on the corrected photoelectric data.

20. The sample analysis system according to claim 19, wherein: The first state information of the sample to be tested is information about an interferent that causes the sample to be tested to have abnormal properties.

21. A sample analysis system, characterized in that: include: A sample preparation device, the sample preparation device is used to prepare a reaction solution using at least part of the sample to be tested and a reaction reagent; A detection device, the detection device is used to detect the sample to be tested to obtain a first detection parameter, and detect the reaction liquid to obtain a second detection parameter, the first detection parameter and the second detection parameter are different; A control device, configured to obtain first state information of the sample to be tested according to the first detection parameter; Obtaining second state information of the reaction liquid according to the second detection parameter; The first state information and the second state information are combined to determine the interferent information of the reaction solution.

22. The sample analysis system according to claim 21, characterized in that: The first state information of the sample to be tested is information about an interferent that causes the sample to be tested to have abnormal properties.

23. A sample analysis system, characterized in that: include: A sample preparation device, the sample preparation device is used to prepare a reaction solution using at least part of the sample to be tested and the reaction reagent in the sample tube; A detection device, the detection device comprising a light source assembly and an optical signal detector; the light source assembly is used to generate irradiation light, the irradiation light forms a first outgoing light after irradiating the reaction liquid, and the optical signal detector collects the first outgoing light to obtain photoelectric data; An image acquisition device, used to acquire an image of the sample tube of the sample to be tested; The control device is configured to obtain first interference information brought by the sample tube according to the image of the sample tube, obtain second interference information of the reaction liquid and the detection result of the detection item of the sample to be tested according to the photoelectric data, and determine the source of the interference of the detection item by combining the first interference information and the second interference information.

24. The sample analysis system according to claim 23, characterized in that: The first interfering substance information includes the chemical reagent type of the chemical reagent contained in the sample tube, and the second interfering substance information includes the chemical reagent type that may cause the test result of the test item to be abnormal; The combining the first interferer information and the second interferer information to determine the interferer source of the detection item includes: If the first interferer information and the second interferer information include the same chemical reagent type, it is determined that the interferer source of the detection item includes a sample tube.

25. The sample analysis system according to claim 24, characterized in that: The image of the sample tube includes an image reflecting the color of the tube cap of the sample tube and / or an image reflecting the color of the label of the sample tube, and the control device determines the type of chemical reagent contained in the sample tube according to the tube cap color and / or the label color.

26. The sample analysis system according to claim 30, characterized in that: The detection item is a creatine kinase item, the first interfering substance information and the second interfering substance information both include anticoagulants, and it is determined that the source of the interfering substance of the creatine kinase item includes a sample tube; The detection project is an α-L-fucosidase project, the first interferor information and the second interferor information both include anticoagulants, and it is determined that the source of the interferor for the α-L-fucosidase project includes a sample tube.

27. A sample analysis method, characterized in that: The sample analysis method comprises: Acquire first state information of the sample to be tested; Acquire photoelectric data of a reaction solution prepared by the sample to be tested and the reagent, and obtain second state information of the reaction solution according to the photoelectric data, wherein the photoelectric data is obtained by collecting the emitted light of a light source irradiating the reaction solution; Determine the interference information of the reaction solution according to the first state information and the second state information; or correct the photoelectric data of the reaction solution according to the first state information and the second state information to obtain the detection result of the detection item.

28. A sample analysis method, characterized in that: include: Acquire a sample image of a sample to be tested; When it is determined according to the sample image of the sample to be tested that the sample to be tested has interference, processing the sample to be tested to remove the interference in the sample to be tested; The processed sample to be tested and the reagent are combined to prepare a reaction solution, and a detection result is obtained according to the photoelectric data of the reaction solution. The photoelectric data is obtained by collecting the emitted light of the reaction solution irradiated by a light source.

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